AlfalfaGEDB | MsG0280011342.01

M. sativa cultivar ZhongmuNo.1 / MsG0280011342.01

KEGG: -
GO: GO:0005515
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011342.01.T01	GAU10984.1	57.72	421	158	12	2	411	7	418	4.49E-145	429

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011342.01.T01	Q8GXC7	25.064	391	224	20	35	389	44	401	2.54E-12	71.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011342.01.T01	A0A2Z6M0S7	57.720	421	158	12	2	411	7	418	2.14e-145	429

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0080048282.01	MsG0280011342.01	PPI
MsG0080048282.01	MsG0280011342.01	PPI
MsG0080048294.01	MsG0280011342.01	PPI
MsG0180000839.01	MsG0280011342.01	PPI
MsG0180000841.01	MsG0280011342.01	PPI
MsG0180001954.01	MsG0280011342.01	PPI
MsG0180002194.01	MsG0280011342.01	PPI
MsG0180002194.01	MsG0280011342.01	PPI
MsG0180002513.01	MsG0280011342.01	PPI
MsG0180002513.01	MsG0280011342.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011342.01.T01	MTR_0129s0070	47.170	424	171	13	8	411	7	397	2.84e-109	327
MsG0280011342.01.T01	MTR_5g096630	43.981	432	191	14	10	411	5	415	1.23e-103	313
MsG0280011342.01.T01	MTR_0129s0120	45.631	412	180	12	15	406	5	392	1.91e-102	310
MsG0280011342.01.T01	MTR_8g063440	44.769	411	187	13	14	411	1	384	1.62e-98	299
MsG0280011342.01.T01	MTR_4g117550	46.265	415	158	16	14	411	8	374	1.25e-97	296
MsG0280011342.01.T01	MTR_5g097330	45.060	415	188	15	18	411	9	404	1.75e-95	292
MsG0280011342.01.T01	MTR_4g102410	45.024	422	188	15	10	408	48	448	2.04e-95	293
MsG0280011342.01.T01	MTR_6g086825	44.444	423	190	12	15	411	58	461	5.64e-95	293
MsG0280011342.01.T01	MTR_6g461660	43.541	418	194	13	11	410	7	400	2.07e-92	284
MsG0280011342.01.T01	MTR_5g069390	42.361	432	198	16	11	410	5	417	3.61e-91	284

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011342.01.T01	AT3G06240	25.064	391	224	20	35	389	44	401	2.59e-13	71.6
MsG0280011342.01.T01	AT4G12560	26.354	277	170	11	162	411	108	377	1.88e-11	65.9
MsG0280011342.01.T01	AT4G12560	26.354	277	170	11	162	411	108	377	1.88e-11	65.9
MsG0280011342.01.T01	AT4G12560	26.354	277	170	11	162	411	108	377	1.88e-11	65.9
MsG0280011342.01.T01	AT2G16810	24.503	302	160	13	15	298	29	280	7.21e-11	63.2

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Find 93 sgRNAs with CRISPR-Local

Find 109 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACGCTTCATTGATTTGAT+TGG	0.371215	2:+82665612	None:intergenic
AAAGTGATACCTTGTGTACC+GGG	0.570653	2:+82665158	None:intergenic
AACTTCTGGTCTGATGGATA+AGG	0.432683	2:-82665742	None:intergenic
AACTTGGTGTGAAGGAATCA+TGG	0.509840	2:-82664735	MsG0280011342.01.T01:CDS
AAGAATGCCGTTAACACTAG+TGG	0.592711	2:+82665314	None:intergenic
AATACAAATTGGACCTTATT+TGG	0.050315	2:+82665261	None:intergenic
AATGGAATGTGTCATTTCAT+GGG	0.610428	2:-82664940	MsG0280011342.01.T01:CDS
AATTGAGGATATTGGTATTA+AGG	0.206316	2:-82664587	MsG0280011342.01.T01:CDS
ACACAAGGTATCACTTTAGT+GGG	0.502595	2:-82665152	MsG0280011342.01.T01:CDS
ACACCCGTGGATGAAAATTC+TGG	0.349134	2:-82664859	MsG0280011342.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACCCAAATAATTGAGGATAT+TGG	-	Chr2:82665650-82665669	MsG0280011342.01.T01:CDS	30.0%
ACTATATATCTCAAACAAGG+GGG	+	Chr2:82665198-82665217	None:intergenic	30.0%
ATAAGGTCCAATTTGTATTG+TGG	-	Chr2:82664988-82665007	MsG0280011342.01.T01:CDS	30.0%
ATATCAATTCTAGGTGAACT+TGG	-	Chr2:82665494-82665513	MsG0280011342.01.T01:CDS	30.0%
CAAATGACATCATCAAACAT+TGG	+	Chr2:82665340-82665359	None:intergenic	30.0%
GACTATATATCTCAAACAAG+GGG	+	Chr2:82665199-82665218	None:intergenic	30.0%
TTAATCCTCCTATCAAAAGA+TGG	+	Chr2:82665724-82665743	None:intergenic	30.0%
TTGTCAGATTGGAATTTACA+AGG	-	Chr2:82665694-82665713	MsG0280011342.01.T01:CDS	30.0%
AGGGCCAAAAGAAAATAGTT+TGG	+	Chr2:82665537-82665556	None:intergenic	35.0%
CCAAGTTAGAGATGACTTTA+AGG	-	Chr2:82665127-82665146	MsG0280011342.01.T01:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	82664516	82665751	82664516	ID=MsG0280011342.01;Name=MsG0280011342.01
Chr2	mRNA	82664516	82665751	82664516	ID=MsG0280011342.01.T01;Parent=MsG0280011342.01;Name=MsG0280011342.01.T01;_AED=0.43;_eAED=0.43;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|411
Chr2	exon	82664516	82665751	82664516	ID=MsG0280011342.01.T01:exon:12336;Parent=MsG0280011342.01.T01
Chr2	CDS	82664516	82665751	82664516	ID=MsG0280011342.01.T01:cds;Parent=MsG0280011342.01.T01

Gene Sequence

>MsG0280011342.01.T01

ATGGATAAGGGAAACAAGTCCTCCGTTGTGGCTGTTGCTGTCACAAATGAAAAAAATAAGGTTAGTTGCAACAAATATATACATGATGATCTTGCATTCTATGTTATTTCAAAACTGCCAATCAAATCAATGAAGCGTTTCGGATGCGTACGCAAATCATGGTCCATCTTGTTTGAAAACTCTCATTTCATGACCATGTACCGCGGCCATTTCATATCTAATAACAACCATTCTGATTCTCAAAATGACTATCATACATTTCTCCTCATACATCATCCTGAACTGCCTGTCATGTTTGATCATCGTTACCATGCAGAATTGTGTTTGCTCTCTAATGAGAGGATTGAGAATAGCGTCAAATTAAGTTTTCCACCTCCATTTCAAAAGAATGACGATAATCTTCGTATTTTGGGCTCCACTAGTGTTAACGGCATTCTTTGTCTCGAGCAACATAGACGAGTAGGAGATCCAAATAAGGTCCAATTTGTATTGTGGAATCCGGCTACTGATGAATCTGTGCTCATTCCTCCCGCCCCTGACGAGTTTGGAGCAGGACACCCTGACTGGTACACCCGGTACACAAGGTATCACTTTAGTGGGTTTGGTTATGACCAAGTTAGAGATGACTTTAAGGTGTTTCAGTATGTAACATTTGATCACATAGACAATGTATCTTATGCCCCCTTGTTTGAGATATATAGTCTAAAAAGTAACTCTTGGAGGATACTCGACATCGATATGCCTAACTTGTATTATGGTCGGCCGGACCAAGGTCTCGACGTGTACATGAATGGAATGTGTCATTTCATGGGTACTAGTGACCAATGTTTGATGATGTCATTTGACTTGAGCAATGAGGTGTTCTTTACAACACCCGTGGATGAAAATTCTGGTTTTGAATTCATTTTCAGATATTTGGTGGTGTTAAATGGGTCAATTGCTTTGATCTCAAATCATTACGACAATGCTCCTTTTAACATATCAATTCTAGGTGAACTTGGTGTGAAGGAATCATGGACCAAACTATTTTCTTTTGGCCCTTTACCTTCCATTGAGTGGCCCCTAATTGGAGTTGGGAAGAAGGGATATATTTTCTTTAGAAAAAATGATGAACTAGCATGTGTTGATTTGAGCACCCAAATAATTGAGGATATTGGTATTAAGGGAGAGCAATTTTGTTGTCAGATTGGAATTTACAAGGAAATCCATCTTTTGATAGGAGGATTAAATAATTAG

Protein sequence

>MsG0280011342.01.T01

MDKGNKSSVVAVAVTNEKNKVSCNKYIHDDLAFYVISKLPIKSMKRFGCVRKSWSILFENSHFMTMYRGHFISNNNHSDSQNDYHTFLLIHHPELPVMFDHRYHAELCLLSNERIENSVKLSFPPPFQKNDDNLRILGSTSVNGILCLEQHRRVGDPNKVQFVLWNPATDESVLIPPAPDEFGAGHPDWYTRYTRYHFSGFGYDQVRDDFKVFQYVTFDHIDNVSYAPLFEIYSLKSNSWRILDIDMPNLYYGRPDQGLDVYMNGMCHFMGTSDQCLMMSFDLSNEVFFTTPVDENSGFEFIFRYLVVLNGSIALISNHYDNAPFNISILGELGVKESWTKLFSFGPLPSIEWPLIGVGKKGYIFFRKNDELACVDLSTQIIEDIGIKGEQFCCQIGIYKEIHLLIGGLNN*