AlfalfaGEDB | MsG0580028186.01

M. sativa cultivar ZhongmuNo.1 / MsG0580028186.01

KEGG: -
GO: GO:0005515
PFAM: PF00646 PF07734

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028186.01.T01	AES98425.1	81.55	271	50	0	87	357	8	278	2.82E-154	445

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028186.01.T01	Q9LU24	25.342	292	195	11	13	299	2	275	1.88E-17	85.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028186.01.T01	G7KDC3	81.550	271	50	0	87	357	8	278	1.35e-154	445

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0080048282.01	MsG0580028186.01	PPI
MsG0080048282.01	MsG0580028186.01	PPI
MsG0080048282.01	MsG0580028186.01	PPI
MsG0080048294.01	MsG0580028186.01	PPI
MsG0180000839.01	MsG0580028186.01	PPI
MsG0180000841.01	MsG0580028186.01	PPI
MsG0180001897.01	MsG0580028186.01	PPI
MsG0180001900.01	MsG0580028186.01	PPI
MsG0180001901.01	MsG0580028186.01	PPI
MsG0180001954.01	MsG0580028186.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028186.01.T01	MTR_5g068490	81.550	271	50	0	87	357	8	278	3.42e-158	445
MsG0580028186.01.T01	MTR_5g043605	59.409	372	88	9	5	356	11	339	2.97e-136	392
MsG0580028186.01.T01	MTR_3g011120	52.000	375	144	14	1	345	1	369	1.27e-107	320
MsG0580028186.01.T01	MTR_1g098180	49.091	385	134	12	7	357	11	367	1.84e-103	309
MsG0580028186.01.T01	MTR_5g024260	49.096	387	148	14	1	341	7	390	1.59e-95	290
MsG0580028186.01.T01	MTR_8g467670	48.138	376	154	13	15	357	13	380	3.03e-95	289
MsG0580028186.01.T01	MTR_2g066170	45.918	392	172	14	1	357	1	387	8.60e-95	288
MsG0580028186.01.T01	MTR_2g066200	46.076	395	169	15	1	357	1	389	4.55e-94	286
MsG0580028186.01.T01	MTR_8g063440	43.333	390	182	10	1	357	1	384	5.28e-94	285
MsG0580028186.01.T01	MTR_0129s0070	44.584	397	179	11	1	357	2	397	9.03e-94	285

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028186.01.T01	AT3G16210	25.342	292	195	11	13	299	2	275	1.92e-18	85.9
MsG0580028186.01.T01	AT2G38590	26.899	316	188	14	24	301	16	326	7.76e-18	84.7
MsG0580028186.01.T01	AT3G17490	23.473	311	216	10	16	311	6	309	3.29e-17	82.4
MsG0580028186.01.T01	AT3G21410	26.298	289	178	12	24	293	46	318	2.45e-15	77.0
MsG0580028186.01.T01	AT3G22350	26.736	288	176	11	19	286	7	279	3.16e-15	76.6
MsG0580028186.01.T01	AT3G17530	27.966	236	154	10	19	241	9	241	5.41e-15	75.9
MsG0580028186.01.T01	AT3G22650	24.658	292	183	11	19	293	12	283	1.67e-14	74.3
MsG0580028186.01.T01	AT3G22730	24.695	328	202	12	19	331	9	306	2.89e-14	73.6
MsG0580028186.01.T01	AT3G17620	24.839	310	200	9	25	309	14	315	3.97e-14	73.6
MsG0580028186.01.T01	AT4G33290	24.272	309	210	12	19	304	8	315	6.74e-14	72.8

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Find 67 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATACAACTACAGTCTTGC+CGG	0.416642	5:-76289739	None:intergenic
AAATTGGCGTCGAGGAATCA+TGG	0.448593	5:+76290222	MsG0580028186.01.T01:CDS
AACTCTTTCTACTTTCTTTC+TGG	0.073703	5:+76289609	MsG0580028186.01.T01:CDS
AACTGATTCCTGGAGGAAAA+TGG	0.293756	5:+76289935	MsG0580028186.01.T01:CDS
AAGAAAATCTTTCTTCCATT+TGG	0.259886	5:+76290419	MsG0580028186.01.T01:CDS
AAGCGCTTAATAGATTTGAG+AGG	0.611953	5:-76289468	None:intergenic
AATGGCATTCTTTGTATCAC+CGG	0.498939	5:+76289720	MsG0580028186.01.T01:CDS
AATGGGAAGAAGGTTAGTAA+CGG	0.666220	5:+76289396	MsG0580028186.01.T01:CDS
AATGGGATATGTCATTGGTT+GGG	0.549037	5:+76290002	MsG0580028186.01.T01:CDS
AATTGGCGTCGAGGAATCAT+GGG	0.582935	5:+76290223	MsG0580028186.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACTCTTTCTACTTTCTTTC+TGG	+	Chr5:76289609-76289628	MsG0580028186.01.T01:CDS	30.0%
AGACTATATATCTCCCAAAA+AGG	-	Chr5:76289912-76289931	None:intergenic	30.0%
ATATCAATCTTGGGTGAAAT+TGG	+	Chr5:76290206-76290225	MsG0580028186.01.T01:CDS	30.0%
GAAAGAAAGTAGAAAGAGTT+CGG	-	Chr5:76289609-76289628	None:intergenic	30.0%
GACTATATATCTCCCAAAAA+GGG	-	Chr5:76289911-76289930	None:intergenic	30.0%
TCTCTTGTGTTTGTAAATCA+TGG	+	Chr5:76289490-76289509	MsG0580028186.01.T01:CDS	30.0%
TTAACCTGCAAGTGTTAAAT+GGG	+	Chr5:76290138-76290157	MsG0580028186.01.T01:CDS	30.0%
TTATGCATCCTATCATAGTA+TGG	+	Chr5:76290279-76290298	MsG0580028186.01.T01:CDS	30.0%
AAATACAACTACAGTCTTGC+CGG	-	Chr5:76289742-76289761	None:intergenic	35.0%
AAGCGCTTAATAGATTTGAG+AGG	-	Chr5:76289471-76289490	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	76289384	76290457	76289384	ID=MsG0580028186.01;Name=MsG0580028186.01
Chr5	mRNA	76289384	76290457	76289384	ID=MsG0580028186.01.T01;Parent=MsG0580028186.01;Name=MsG0580028186.01.T01;_AED=0.18;_eAED=0.18;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|357
Chr5	exon	76289384	76290457	76289384	ID=MsG0580028186.01.T01:exon:24000;Parent=MsG0580028186.01.T01
Chr5	CDS	76289384	76290457	76289384	ID=MsG0580028186.01.T01:cds;Parent=MsG0580028186.01.T01

Gene Sequence

>MsG0580028186.01.T01

ATGGCTTCAACGAATGGGAAGAAGGTTAGTAACGGTAGTAACTTTGTTCATGATGACATTGCTTTGTGTATTCTTTCTAAACTTCCTCTCAAATCTATTAAGCGCTTCTCTTGTGTTTGTAAATCATGGTCTCTTTTATTTGAAAACCCTAATTTCATCAAAATGTTCATTTCCAATTCTCATCCACTCTATCATGATGCTTTTCTCATTTTAAATCAGTATCCGAACTCTTTCTACTTTCTTTCTGGTCACAAGTTTCAGAATAAACTCAAATTGAAATTTCCACCTCCATTTCTCGATTCACAGCTTTCCATTAGTCGAATTATAGATTCTGATAATGGCATTCTTTGTATCACCGGCAAGACTGTAGTTGTATTTTGGAATCCTGCTACTGAGGAAGTTAAGGTCATTCCTCCTTCTCAACTTGAGTTTTCCTGTGGAGTTATGGATCATGGATTTGGCTATGACCATGTTAGGGATGACTATAAGCTCATCCAATATGTGGATGTTGTGGAGGGTCATGACCCTTTTTGGGAGATATATAGTCTAAAAACTGATTCCTGGAGGAAAATGGATATTGATATGCCAATTTGTGTAAATGATGATAATGTGTGCTTGAATGGGATATGTCATTGGTTGGGTGAAGAAAATGATGACTATGATATGGTTCTTGTGTCATTTCACTTGGGCAATGAGGTGTGCTTTGTTACACCTTTACCCTTTTTAGAAGGCAAGGATTATGATTTTGATGATGTTAACCTGCAAGTGTTAAATGGGTCGGTTGCTGTGATATTTAATCATATAGAGACAAAGTCTTTTCACATATCAATCTTGGGTGAAATTGGCGTCGAGGAATCATGGGTTAGACTCTTTGATATTGGACCTTTGTCTTGCATTATGCATCCTATCATAGTATGGAAGAAGGGCAGTTTAATTGTTAGAAAGTTTGATCAACTAGCTTGTTTGGATCTAACTACCGGAGTGATTGAGGAGATTGGTGTTAAAGCAGAACATTTTTGCGACATTTTCATTTACAAGAAAATCTTTCTTCCATTTGGAGTTATTGACAATTAA

Protein sequence

>MsG0580028186.01.T01

MASTNGKKVSNGSNFVHDDIALCILSKLPLKSIKRFSCVCKSWSLLFENPNFIKMFISNSHPLYHDAFLILNQYPNSFYFLSGHKFQNKLKLKFPPPFLDSQLSISRIIDSDNGILCITGKTVVVFWNPATEEVKVIPPSQLEFSCGVMDHGFGYDHVRDDYKLIQYVDVVEGHDPFWEIYSLKTDSWRKMDIDMPICVNDDNVCLNGICHWLGEENDDYDMVLVSFHLGNEVCFVTPLPFLEGKDYDFDDVNLQVLNGSVAVIFNHIETKSFHISILGEIGVEESWVRLFDIGPLSCIMHPIIVWKKGSLIVRKFDQLACLDLTTGVIEEIGVKAEHFCDIFIYKKIFLPFGVIDN*