Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048856.01 | MsG0580028479.01 | 0.807897 | 4.038559e-50 | 2.276370e-47 |
| MsG0180000145.01 | MsG0580028479.01 | 0.819022 | 1.444740e-52 | 1.099109e-49 |
| MsG0180000210.01 | MsG0580028479.01 | 0.800319 | 1.524164e-48 | 7.075673e-46 |
| MsG0180000335.01 | MsG0580028479.01 | 0.814580 | 1.432822e-51 | 9.651219e-49 |
| MsG0180000470.01 | MsG0580028479.01 | 0.845556 | 3.772937e-59 | 6.311259e-56 |
| MsG0180000550.01 | MsG0580028479.01 | 0.803601 | 3.225530e-49 | 1.627748e-46 |
| MsG0180000828.01 | MsG0580028479.01 | 0.814092 | 1.837442e-51 | 1.221355e-48 |
| MsG0180001063.01 | MsG0580028479.01 | 0.837731 | 4.372050e-57 | 5.731353e-54 |
| MsG0180003669.01 | MsG0580028479.01 | 0.804932 | 1.703466e-49 | 8.896412e-47 |
| MsG0180003673.01 | MsG0580028479.01 | 0.828736 | 7.616060e-55 | 7.635305e-52 |
| MsG0180003686.01 | MsG0580028479.01 | 0.826500 | 2.621921e-54 | 2.463150e-51 |
| MsG0180003773.01 | MsG0580028479.01 | 0.804992 | 1.655210e-49 | 8.657143e-47 |
| MsG0180004465.01 | MsG0580028479.01 | 0.802144 | 6.450096e-49 | 3.135937e-46 |
| MsG0180004765.01 | MsG0580028479.01 | 0.804548 | 2.049047e-49 | 1.059465e-46 |
| MsG0180004926.01 | MsG0580028479.01 | 0.828992 | 6.602859e-55 | 6.669349e-52 |
| MsG0180005050.01 | MsG0580028479.01 | 0.835446 | 1.670598e-56 | 2.043330e-53 |
| MsG0280010722.01 | MsG0580028479.01 | 0.814097 | 1.832659e-51 | 1.218355e-48 |
| MsG0280011101.01 | MsG0580028479.01 | 0.805166 | 1.521731e-49 | 7.994838e-47 |
| MsG0380011609.01 | MsG0580028479.01 | 0.840775 | 7.094452e-58 | 1.021213e-54 |
| MsG0380012067.01 | MsG0580028479.01 | 0.802691 | 4.975028e-49 | 2.452786e-46 |
| MsG0380012460.01 | MsG0580028479.01 | 0.816282 | 5.992080e-52 | 4.228079e-49 |
| MsG0380013834.01 | MsG0580028479.01 | 0.804440 | 2.157633e-49 | 1.112526e-46 |
| MsG0380014251.01 | MsG0580028479.01 | 0.800493 | 1.404812e-48 | 6.550255e-46 |
| MsG0380014468.01 | MsG0580028479.01 | 0.819778 | 9.713663e-53 | 7.546488e-50 |
| MsG0380014537.01 | MsG0580028479.01 | 0.800677 | 1.287993e-48 | 6.033809e-46 |
| MsG0380014573.01 | MsG0580028479.01 | 0.811811 | 5.807315e-51 | 3.630403e-48 |
| MsG0380014706.01 | MsG0580028479.01 | 0.809234 | 2.093191e-50 | 1.222351e-47 |
| MsG0480018465.01 | MsG0580028479.01 | 0.815031 | 1.138250e-51 | 7.760963e-49 |
| MsG0480018653.01 | MsG0580028479.01 | 0.811240 | 7.729248e-51 | 4.759436e-48 |
| MsG0480018883.01 | MsG0580028479.01 | 0.812030 | 5.205113e-51 | 3.272952e-48 |
| MsG0480018964.01 | MsG0580028479.01 | 0.805975 | 1.029585e-49 | 5.522852e-47 |
| MsG0480019206.01 | MsG0580028479.01 | 0.806804 | 6.885801e-50 | 3.772680e-47 |
| MsG0480019297.01 | MsG0580028479.01 | 0.826111 | 3.245130e-54 | 3.015122e-51 |
| MsG0480021215.01 | MsG0580028479.01 | 0.808620 | 2.832316e-50 | 1.627037e-47 |
| MsG0480021465.01 | MsG0580028479.01 | 0.823557 | 1.299110e-53 | 1.122310e-50 |
| MsG0480021491.01 | MsG0580028479.01 | 0.811992 | 5.303787e-51 | 3.331787e-48 |
| MsG0480021534.01 | MsG0580028479.01 | 0.805276 | 1.443106e-49 | 7.602874e-47 |
| MsG0480021536.01 | MsG0580028479.01 | 0.802035 | 6.789177e-49 | 3.291720e-46 |
| MsG0480021796.01 | MsG0580028479.01 | 0.801074 | 1.069040e-48 | 5.058844e-46 |
| MsG0480021853.01 | MsG0580028479.01 | 0.808661 | 2.775650e-50 | 1.596240e-47 |
| MsG0480021925.01 | MsG0580028479.01 | 0.808308 | 3.301207e-50 | 1.881140e-47 |
| MsG0480022068.01 | MsG0580028479.01 | 0.839318 | 1.702356e-57 | 2.342260e-54 |
| MsG0480022213.01 | MsG0580028479.01 | -0.813162 | 2.942805e-51 | 1.907253e-48 |
| MsG0480022222.01 | MsG0580028479.01 | 0.807555 | 4.773865e-50 | 2.667002e-47 |
| MsG0480022985.01 | MsG0580028479.01 | 0.809459 | 1.872217e-50 | 1.099849e-47 |
| MsG0480023463.01 | MsG0580028479.01 | 0.812135 | 4.936624e-51 | 3.113247e-48 |
| MsG0480023488.01 | MsG0580028479.01 | 0.801372 | 9.287870e-49 | 4.428223e-46 |
| MsG0580024038.01 | MsG0580028479.01 | 0.836220 | 1.063408e-56 | 1.331579e-53 |
| MsG0580024552.01 | MsG0580028479.01 | 0.829893 | 3.988472e-55 | 4.136969e-52 |
| MsG0580025758.01 | MsG0580028479.01 | 0.822568 | 2.209621e-53 | 1.856496e-50 |
| MsG0580025987.01 | MsG0580028479.01 | 0.827696 | 1.356440e-54 | 1.319301e-51 |
| MsG0380015332.01 | MsG0580028479.01 | 0.811685 | 6.187213e-51 | 3.854792e-48 |
| MsG0380015555.01 | MsG0580028479.01 | 0.822407 | 2.407934e-53 | 2.013817e-50 |
| MsG0380015566.01 | MsG0580028479.01 | 0.809561 | 1.780200e-50 | 1.048668e-47 |
| MsG0380015720.01 | MsG0580028479.01 | 0.806086 | 9.757961e-50 | 5.249043e-47 |
| MsG0380016064.01 | MsG0580028479.01 | 0.826935 | 2.064924e-54 | 1.964453e-51 |
| MsG0380016517.01 | MsG0580028479.01 | 0.829324 | 5.485673e-55 | 5.596667e-52 |
| MsG0380016910.01 | MsG0580028479.01 | 0.825248 | 5.198809e-54 | 4.713145e-51 |
| MsG0380017048.01 | MsG0580028479.01 | 0.803832 | 2.887746e-49 | 1.465969e-46 |
| MsG0380017244.01 | MsG0580028479.01 | 0.806158 | 9.422909e-50 | 5.078257e-47 |
| MsG0380017461.01 | MsG0580028479.01 | 0.814668 | 1.370588e-51 | 9.253443e-49 |
| MsG0380017543.01 | MsG0580028479.01 | 0.806515 | 7.925366e-50 | 4.310074e-47 |
| MsG0380017584.01 | MsG0580028479.01 | 0.801635 | 8.203211e-49 | 3.937042e-46 |
| MsG0380017589.01 | MsG0580028479.01 | 0.828180 | 1.037606e-54 | 1.023650e-51 |
| MsG0380017680.01 | MsG0580028479.01 | 0.804170 | 2.456684e-49 | 1.257971e-46 |
| MsG0380018075.01 | MsG0580028479.01 | 0.804595 | 2.003553e-49 | 1.037210e-46 |
| MsG0480018163.01 | MsG0580028479.01 | 0.812149 | 4.902253e-51 | 3.092806e-48 |
| MsG0480018183.01 | MsG0580028479.01 | 0.803119 | 4.057774e-49 | 2.022439e-46 |
| MsG0480018236.01 | MsG0580028479.01 | 0.810855 | 9.364944e-51 | 5.708586e-48 |
| MsG0580026086.01 | MsG0580028479.01 | 0.811053 | 8.486519e-51 | 5.200338e-48 |
| MsG0580026131.01 | MsG0580028479.01 | 0.818199 | 2.219880e-52 | 1.650744e-49 |
| MsG0580026726.01 | MsG0580028479.01 | 0.811169 | 8.009399e-51 | 4.922648e-48 |
| MsG0580027031.01 | MsG0580028479.01 | 0.800631 | 1.316378e-48 | 6.159578e-46 |
| MsG0580028479.01 | MsG0580028996.01 | 0.806502 | 7.974206e-50 | 4.335203e-47 |
| MsG0580028479.01 | MsG0580029008.01 | 0.823733 | 1.181496e-53 | 1.026003e-50 |
| MsG0580028479.01 | MsG0580029236.01 | 0.819307 | 1.243916e-52 | 9.536693e-50 |
| MsG0580028479.01 | MsG0580029826.01 | 0.800847 | 1.189181e-48 | 5.595090e-46 |
| MsG0580028479.01 | MsG0580029925.01 | 0.823226 | 1.552259e-53 | 1.328543e-50 |
| MsG0580028479.01 | MsG0680030597.01 | 0.810898 | 9.168673e-51 | 5.595322e-48 |
| MsG0580028479.01 | MsG0680030880.01 | 0.806678 | 7.321170e-50 | 3.997967e-47 |
| MsG0580028479.01 | MsG0680030882.01 | 0.820125 | 8.092756e-53 | 6.348754e-50 |
| MsG0580028479.01 | MsG0680031087.01 | 0.802138 | 6.468670e-49 | 3.144491e-46 |
| MsG0580028479.01 | MsG0680031262.01 | 0.806159 | 9.417133e-50 | 5.075294e-47 |
| MsG0580028479.01 | MsG0680032771.01 | 0.802542 | 5.340447e-49 | 2.622920e-46 |
| MsG0580028479.01 | MsG0680033928.01 | 0.801479 | 8.829947e-49 | 4.221280e-46 |
| MsG0580028479.01 | MsG0680034234.01 | 0.804596 | 2.002072e-49 | 1.036482e-46 |
| MsG0580028479.01 | MsG0680034739.01 | 0.804488 | 2.109365e-49 | 1.088954e-46 |
| MsG0580028479.01 | MsG0680035475.01 | 0.808684 | 2.744737e-50 | 1.579408e-47 |
| MsG0580028479.01 | MsG0680035692.01 | 0.802294 | 6.006144e-49 | 2.931430e-46 |
| MsG0580028479.01 | MsG0780035948.01 | 0.825799 | 3.849806e-54 | 3.545463e-51 |
| MsG0580028479.01 | MsG0780036018.01 | 0.817754 | 2.798162e-52 | 2.055583e-49 |
| MsG0580028479.01 | MsG0780036190.01 | 0.809744 | 1.626105e-50 | 9.626008e-48 |
| MsG0580028479.01 | MsG0780037054.01 | 0.809909 | 1.499032e-50 | 8.911588e-48 |
| MsG0580028479.01 | MsG0780037241.01 | 0.808682 | 2.746445e-50 | 1.580334e-47 |
| MsG0580028479.01 | MsG0780038276.01 | 0.811221 | 7.803951e-51 | 4.803121e-48 |
| MsG0580028479.01 | MsG0780038391.01 | 0.814679 | 1.362908e-51 | 9.204509e-49 |
| MsG0580028479.01 | MsG0780038401.01 | 0.819633 | 1.048578e-52 | 8.112838e-50 |
| MsG0580028479.01 | MsG0780038602.01 | 0.802962 | 4.373658e-49 | 2.171204e-46 |
| MsG0580028479.01 | MsG0780039170.01 | 0.808871 | 2.503130e-50 | 1.447514e-47 |
| MsG0580028479.01 | MsG0780039443.01 | 0.804883 | 1.744615e-49 | 9.100018e-47 |
| MsG0580028479.01 | MsG0780039520.01 | 0.818811 | 1.613403e-52 | 1.220135e-49 |
| MsG0580028479.01 | MsG0780039522.01 | 0.821392 | 4.136384e-53 | 3.360935e-50 |
| MsG0580028479.01 | MsG0780040057.01 | 0.828920 | 6.876273e-55 | 6.930852e-52 |
| MsG0580028479.01 | MsG0780040074.01 | 0.825133 | 5.534915e-54 | 5.001665e-51 |
| MsG0580028479.01 | MsG0780040596.01 | 0.812653 | 3.804340e-51 | 2.432564e-48 |
| MsG0580028479.01 | MsG0780040888.01 | 0.806085 | 9.763942e-50 | 5.252128e-47 |
| MsG0580028479.01 | MsG0780041416.01 | 0.818329 | 2.075271e-52 | 1.548764e-49 |
| MsG0580028479.01 | MsG0780041456.01 | 0.829385 | 5.302604e-55 | 5.419051e-52 |
| MsG0580028479.01 | MsG0780041507.01 | 0.800487 | 1.408549e-48 | 6.566681e-46 |
| MsG0580028479.01 | MsG0880041836.01 | 0.805759 | 1.143315e-49 | 6.099163e-47 |
| MsG0580028479.01 | MsG0880042520.01 | 0.802997 | 4.300562e-49 | 2.136742e-46 |
| MsG0580028479.01 | MsG0880042698.01 | 0.814312 | 1.642794e-51 | 1.098584e-48 |
| MsG0580028479.01 | MsG0880042966.01 | 0.825770 | 3.911364e-54 | 3.599112e-51 |
| MsG0580028479.01 | MsG0880043509.01 | 0.801547 | 8.554071e-49 | 4.096240e-46 |
| MsG0580028479.01 | MsG0880043941.01 | 0.816683 | 4.873097e-52 | 3.476107e-49 |
| MsG0580028479.01 | MsG0880044574.01 | 0.821723 | 3.468725e-53 | 2.844735e-50 |
| MsG0580028479.01 | MsG0880044728.01 | 0.812212 | 4.748979e-51 | 3.001267e-48 |
| MsG0580028479.01 | MsG0880045111.01 | 0.812282 | 4.585843e-51 | 2.903685e-48 |
| MsG0580028479.01 | MsG0880045362.01 | 0.811305 | 7.482519e-51 | 4.615229e-48 |
| MsG0580028479.01 | MsG0880045736.01 | 0.828981 | 6.642701e-55 | 6.707080e-52 |
| MsG0580028479.01 | MsG0880045765.01 | 0.804825 | 1.793485e-49 | 9.340717e-47 |
| MsG0580028479.01 | MsG0880045895.01 | 0.818056 | 2.392231e-52 | 1.771841e-49 |
| MsG0580028479.01 | MsG0880045896.01 | 0.822369 | 2.457290e-53 | 2.052910e-50 |
| MsG0580028479.01 | MsG0880045937.01 | 0.819369 | 1.203943e-52 | 9.246303e-50 |
| MsG0580028479.01 | MsG0880046645.01 | 0.818169 | 2.255348e-52 | 1.675699e-49 |
| MsG0280006601.01 | MsG0580028479.01 | 0.809342 | 1.984491e-50 | 1.162196e-47 |
| MsG0280006770.01 | MsG0580028479.01 | 0.802626 | 5.132239e-49 | 2.526071e-46 |
| MsG0280007064.01 | MsG0580028479.01 | 0.801925 | 7.154818e-49 | 3.459206e-46 |
| MsG0280007066.01 | MsG0580028479.01 | 0.819619 | 1.056274e-52 | 8.169034e-50 |
| MsG0280007547.01 | MsG0580028479.01 | 0.806098 | 9.702938e-50 | 5.220945e-47 |
| MsG0280007955.01 | MsG0580028479.01 | 0.826502 | 2.618730e-54 | 2.460333e-51 |
| MsG0280008130.01 | MsG0580028479.01 | 0.803075 | 4.145470e-49 | 2.063710e-46 |
| MsG0280008377.01 | MsG0580028479.01 | 0.804115 | 2.522558e-49 | 1.289898e-46 |
| MsG0280008378.01 | MsG0580028479.01 | 0.813366 | 2.654250e-51 | 1.730018e-48 |
| MsG0280009748.01 | MsG0580028479.01 | 0.843532 | 1.322188e-58 | 2.074744e-55 |
| MsG0280010184.01 | MsG0580028479.01 | 0.801801 | 7.587089e-49 | 3.656708e-46 |
| MsG0280010349.01 | MsG0580028479.01 | 0.808857 | 2.520620e-50 | 1.457086e-47 |
| MsG0280010443.01 | MsG0580028479.01 | 0.834870 | 2.333652e-56 | 2.805468e-53 |
| MsG0280010517.01 | MsG0580028479.01 | 0.802646 | 5.083750e-49 | 2.503403e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTTGCAGTTTCGCCACTTT+GGG | 0.336638 | 5:+81160454 | None:intergenic |
| TCTGAGTAGCCCATGGAGTT+TGG | 0.368716 | 5:+81160295 | None:intergenic |
| AGCTTGCAGTTTCGCCACTT+TGG | 0.418950 | 5:+81160453 | None:intergenic |
| ACCTTCATCAAGCTTGTTGA+CGG | 0.420657 | 5:-81160246 | None:intergenic |
| TCGTTTGTCTGTAGAGAAAG+TGG | 0.479982 | 5:-81160422 | MsG0580028479.01.T01:CDS |
| TCCGTCAACAAGCTTGATGA+AGG | 0.482026 | 5:+81160245 | None:intergenic |
| CTGGCGGAGACCAAACTCCA+TGG | 0.521300 | 5:-81160305 | MsG0580028479.01.T01:CDS |
| TGGTACAAGCTTGTTTGAGA+AGG | 0.522253 | 5:+81160345 | None:intergenic |
| GACATGCTCATCTTCAAAGT+CGG | 0.556826 | 5:+81160504 | None:intergenic |
| TGGATGTAGTTAAGACAGAG+AGG | 0.583862 | 5:-81160402 | MsG0580028479.01.T01:CDS |
| GAAGATGAGCATGTCAAAGA+CGG | 0.589931 | 5:-81160496 | MsG0580028479.01.T01:CDS |
| TGGCGGAGACCAAACTCCAT+GGG | 0.599923 | 5:-81160304 | MsG0580028479.01.T01:CDS |
| GTGGTTCTCTGAGTAGCCCA+TGG | 0.599947 | 5:+81160288 | None:intergenic |
| TCAGACGTTCGTGCGTGGTG+TGG | 0.602586 | 5:+81160269 | None:intergenic |
| AAGGTTCAGACGTTCGTGCG+TGG | 0.606833 | 5:+81160264 | None:intergenic |
| AAAAGTTTCAACAATTCTGG+CGG | 0.630996 | 5:-81160321 | MsG0580028479.01.T01:CDS |
| TCACTCTTCACATCCCAAAG+TGG | 0.645594 | 5:-81160467 | MsG0580028479.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CAAAAAAGTTTCAACAATTC+TGG | - | Chr5:81160463-81160482 | MsG0580028479.01.T01:CDS | 25.0% |
| !!! | CAGAATTGTTGAAACTTTTT+TGG | + | Chr5:81160465-81160484 | None:intergenic | 25.0% |
| AAAAGTTTCAACAATTCTGG+CGG | - | Chr5:81160466-81160485 | MsG0580028479.01.T01:CDS | 30.0% | |
| GAAGATGAGCATGTCAAAGA+CGG | - | Chr5:81160291-81160310 | MsG0580028479.01.T01:CDS | 40.0% | |
| GACATGCTCATCTTCAAAGT+CGG | + | Chr5:81160286-81160305 | None:intergenic | 40.0% | |
| TCGTTTGTCTGTAGAGAAAG+TGG | - | Chr5:81160365-81160384 | MsG0580028479.01.T01:CDS | 40.0% | |
| TGGATGTAGTTAAGACAGAG+AGG | - | Chr5:81160385-81160404 | MsG0580028479.01.T01:CDS | 40.0% | |
| ! | TGGTACAAGCTTGTTTGAGA+AGG | + | Chr5:81160445-81160464 | None:intergenic | 40.0% |
| TCACTCTTCACATCCCAAAG+TGG | - | Chr5:81160320-81160339 | MsG0580028479.01.T01:CDS | 45.0% | |
| AGCTTGCAGTTTCGCCACTT+TGG | + | Chr5:81160337-81160356 | None:intergenic | 50.0% | |
| TCTGAGTAGCCCATGGAGTT+TGG | + | Chr5:81160495-81160514 | None:intergenic | 50.0% | |
| ! | GCTTGCAGTTTCGCCACTTT+GGG | + | Chr5:81160336-81160355 | None:intergenic | 50.0% |
| AAGGTTCAGACGTTCGTGCG+TGG | + | Chr5:81160526-81160545 | None:intergenic | 55.0% | |
| GTGGTTCTCTGAGTAGCCCA+TGG | + | Chr5:81160502-81160521 | None:intergenic | 55.0% | |
| TGGCGGAGACCAAACTCCAT+GGG | - | Chr5:81160483-81160502 | MsG0580028479.01.T01:CDS | 55.0% | |
| CTGGCGGAGACCAAACTCCA+TGG | - | Chr5:81160482-81160501 | MsG0580028479.01.T01:CDS | 60.0% | |
| TCAGACGTTCGTGCGTGGTG+TGG | + | Chr5:81160521-81160540 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 81160249 | 81160560 | 81160249 | ID=MsG0580028479.01;Name=MsG0580028479.01 |
| Chr5 | mRNA | 81160249 | 81160560 | 81160249 | ID=MsG0580028479.01.T01;Parent=MsG0580028479.01;Name=MsG0580028479.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|103 |
| Chr5 | exon | 81160249 | 81160560 | 81160249 | ID=MsG0580028479.01.T01:exon:5933;Parent=MsG0580028479.01.T01 |
| Chr5 | CDS | 81160249 | 81160560 | 81160249 | ID=MsG0580028479.01.T01:cds;Parent=MsG0580028479.01.T01 |
| Gene Sequence |
| Protein sequence |
