AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0079040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0079040 MtrunA17_Chr1g0150191 97.731 617 14 0 1 617 1 617 0.0 1237
Msa0079040 MtrunA17_Chr5g0401341 50.774 646 273 13 1 617 1 630 0.0 593
Msa0079040 MtrunA17_Chr1g0207461 48.523 643 287 15 1 617 1 625 0.0 551
Msa0079040 MtrunA17_Chr8g0367891 41.129 620 303 13 1 582 1 596 2.27e-136 410
Msa0079040 MtrunA17_Chr4g0076521 39.046 566 298 11 1 546 1 539 2.60e-134 405
Msa0079040 MtrunA17_Chr5g0412591 43.667 529 227 11 1 493 1 494 1.26e-132 401
Msa0079040 MtrunA17_Chr2g0279481 38.262 541 273 9 6 492 19 552 4.29e-123 379
Msa0079040 MtrunA17_Chr4g0050021 38.964 521 265 9 22 499 39 549 8.76e-123 378
Msa0079040 MtrunA17_Chr2g0290741 36.086 557 314 9 1 548 1 524 6.61e-119 365
Msa0079040 MtrunA17_Chr2g0285511 40.748 508 279 11 1 492 1 502 2.24e-116 359
Msa0079040 MtrunA17_Chr2g0303541 35.974 606 350 12 1 580 1 594 9.11e-116 359
Msa0079040 MtrunA17_Chr1g0181181 37.984 516 271 11 1 496 1 487 4.73e-104 326
Msa0079040 MtrunA17_Chr8g0382231 33.645 535 272 10 21 517 46 535 5.49e-104 328
Msa0079040 MtrunA17_Chr5g0401451 37.072 526 296 14 23 535 36 539 2.31e-102 323
Msa0079040 MtrunA17_Chr2g0320691 34.927 481 289 6 25 487 21 495 1.17e-97 311
Msa0079040 MtrunA17_Chr8g0374011 32.843 612 358 14 22 585 15 621 3.91e-97 311
Msa0079040 MtrunA17_Chr4g0010091 36.800 500 290 9 18 506 25 509 4.27e-97 310
Msa0079040 MtrunA17_Chr4g0053841 34.991 543 313 13 24 546 10 532 1.71e-95 305
Msa0079040 MtrunA17_Chr7g0245201 33.333 627 351 15 19 617 53 640 1.39e-92 298
Msa0079040 MtrunA17_Chr1g0195911 36.076 474 280 7 25 488 34 494 1.20e-89 290
Msa0079040 MtrunA17_Chr1g0199631 38.889 468 261 13 45 496 8 466 1.84e-87 283
Msa0079040 MtrunA17_Chr7g0268781 34.490 490 294 10 25 502 34 508 1.22e-82 272
Msa0079040 MtrunA17_Chr3g0108331 33.402 488 276 9 12 488 11 460 5.09e-80 263
Msa0079040 MtrunA17_Chr1g0191711 31.677 483 274 8 28 501 59 494 4.34e-79 260
Msa0079040 MtrunA17_Chr5g0432861 29.821 503 297 10 12 495 12 477 5.01e-73 245
Msa0079040 MtrunA17_Chr2g0332281 40.484 289 140 7 213 488 24 293 1.08e-56 197
Msa0079040 MtrunA17_Chr4g0003891 39.792 289 140 8 213 488 46 313 2.68e-53 189
Msa0079040 MtrunA17_Chr4g0037661 26.682 446 281 11 69 492 49 470 1.01e-38 150
Msa0079040 MtrunA17_Chr1g0163311 25.933 509 312 16 30 508 6 479 1.07e-38 149
Msa0079040 MtrunA17_Chr4g0023801 25.420 476 319 10 36 505 14 459 9.68e-38 147
Msa0079040 MtrunA17_Chr1g0206621 34.302 172 100 2 35 204 76 236 4.31e-28 112
Msa0079040 MtrunA17_Chr2g0280541 25.385 390 251 9 93 474 1 358 7.85e-23 101
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0079040 AT4G31820.1 57.966 590 187 13 1 581 1 538 0.0 651
Msa0079040 AT4G31820.2 56.797 537 171 13 54 581 1 485 0.0 572
Msa0079040 AT2G14820.2 52.906 499 223 4 1 493 1 493 1.20e-179 523
Msa0079040 AT2G14820.1 52.906 499 223 4 1 493 1 493 1.20e-179 523
Msa0079040 AT5G67440.3 46.942 605 272 11 1 597 1 564 4.27e-175 509
Msa0079040 AT5G67440.1 46.942 605 272 11 1 597 1 564 4.27e-175 509
Msa0079040 AT4G37590.1 46.387 595 248 16 1 573 1 546 1.00e-167 491
Msa0079040 AT2G23050.1 48.406 502 211 9 1 497 1 459 1.42e-157 461
Msa0079040 AT5G67440.2 45.213 564 260 11 42 597 5 527 2.33e-150 445
Msa0079040 AT1G67900.5 45.333 525 242 9 1 492 1 513 3.46e-142 427
Msa0079040 AT1G67900.1 45.333 525 242 9 1 492 1 513 3.46e-142 427
Msa0079040 AT1G67900.3 45.333 525 242 9 1 492 1 513 3.46e-142 427
Msa0079040 AT1G67900.6 45.333 525 242 9 1 492 1 513 3.46e-142 427
Msa0079040 AT1G67900.2 45.333 525 242 9 1 492 1 513 3.46e-142 427
Msa0079040 AT1G67900.4 45.333 525 242 9 1 492 1 513 3.46e-142 427
Msa0079040 AT3G26490.1 41.426 519 265 12 1 490 1 509 2.79e-124 379
Msa0079040 AT3G26490.3 41.426 519 265 12 1 490 1 509 2.79e-124 379
Msa0079040 AT3G26490.4 41.426 519 265 12 1 490 1 509 2.79e-124 379
Msa0079040 AT5G47800.3 38.067 507 286 6 1 499 1 487 6.07e-123 375
Msa0079040 AT5G47800.1 38.067 507 286 6 1 499 1 487 6.07e-123 375
Msa0079040 AT5G47800.4 38.067 507 286 6 1 499 1 487 6.07e-123 375
Msa0079040 AT5G47800.2 38.067 507 286 6 1 499 114 600 2.01e-121 375
Msa0079040 AT5G03250.1 39.038 520 283 11 1 496 1 510 4.54e-115 356
Msa0079040 AT2G23050.2 43.523 386 175 7 112 497 1 343 6.66e-105 322
Msa0079040 AT1G30440.1 35.922 515 304 8 1 492 1 512 8.93e-104 329
Msa0079040 AT3G26490.2 40.558 466 238 12 1 437 1 456 1.90e-102 320
Msa0079040 AT5G13600.1 37.500 536 294 15 1 512 1 519 4.29e-101 320
Msa0079040 AT5G48800.1 38.316 475 267 7 22 492 36 488 5.23e-97 310
Msa0079040 AT5G67385.1 36.786 473 271 9 24 488 22 474 1.49e-96 308
Msa0079040 AT3G44820.1 33.603 494 297 8 22 487 18 508 4.25e-91 295
Msa0079040 AT1G03010.3 35.863 527 288 14 12 511 18 521 1.84e-89 290
Msa0079040 AT5G66560.1 34.615 546 276 12 22 500 14 545 3.34e-89 290
Msa0079040 AT1G03010.2 35.938 512 280 13 25 511 7 495 7.53e-89 288
Msa0079040 AT1G03010.1 35.938 512 280 13 25 511 34 522 1.28e-88 288
Msa0079040 AT3G08660.1 33.450 571 313 12 19 567 26 551 4.61e-88 285
Msa0079040 AT5G10250.2 33.925 507 250 11 22 488 45 506 7.47e-88 286
Msa0079040 AT2G47860.1 37.344 482 262 11 25 488 35 494 2.52e-87 285
Msa0079040 AT3G08570.1 34.146 574 332 14 19 567 26 578 1.52e-86 282
Msa0079040 AT5G66560.3 34.528 530 266 11 38 500 1 516 5.18e-86 281
Msa0079040 AT5G67385.4 36.526 449 257 9 48 488 1 429 8.33e-86 278
Msa0079040 AT5G67385.5 36.526 449 257 9 48 488 1 429 8.33e-86 278
Msa0079040 AT5G67385.2 36.526 449 257 9 48 488 1 429 8.33e-86 278
Msa0079040 AT5G67385.3 36.526 449 257 9 48 488 1 429 8.33e-86 278
Msa0079040 AT5G10250.1 33.468 496 252 10 22 488 45 491 5.60e-85 278
Msa0079040 AT5G66560.4 37.363 455 248 10 69 500 66 506 3.10e-83 274
Msa0079040 AT3G50840.1 35.000 480 268 8 22 488 11 459 7.98e-83 271
Msa0079040 AT2G47860.3 35.644 505 262 12 25 488 35 517 2.78e-82 272
Msa0079040 AT2G30520.1 32.803 503 278 10 4 488 9 469 1.44e-80 266
Msa0079040 AT3G08660.3 33.080 526 286 11 64 567 10 491 2.18e-77 255
Msa0079040 AT3G49970.1 36.180 445 220 11 38 473 5 394 5.08e-76 251
Msa0079040 AT3G49970.2 36.180 445 220 11 38 473 5 394 5.08e-76 251
Msa0079040 AT3G50840.2 34.361 454 254 8 48 488 1 423 2.86e-73 245
Msa0079040 AT3G50840.3 34.361 454 254 8 48 488 1 423 2.86e-73 245
Msa0079040 AT5G03250.2 36.609 407 227 9 112 496 1 398 4.54e-73 243
Msa0079040 AT2G30520.2 33.043 460 250 11 47 488 2 421 2.19e-71 240
Msa0079040 AT2G30520.3 33.043 460 250 11 47 488 2 421 2.19e-71 240
Msa0079040 AT3G08660.2 34.434 424 239 10 19 437 26 415 1.66e-65 221
Msa0079040 AT2G47860.4 35.499 431 238 11 25 437 35 443 2.83e-65 221
Msa0079040 AT5G66560.2 31.875 480 249 10 22 437 14 479 2.62e-64 219
Msa0079040 AT5G64330.2 39.773 264 143 3 7 256 33 294 2.54e-62 218
Msa0079040 AT5G64330.2 37.959 245 132 5 271 499 373 613 3.76e-43 165
Msa0079040 AT5G64330.1 39.773 264 143 3 7 256 33 294 7.56e-62 219
Msa0079040 AT5G64330.1 37.959 245 132 5 271 499 373 613 3.63e-43 166
Msa0079040 AT2G47860.6 33.700 454 238 12 25 437 35 466 2.13e-60 209
Msa0079040 AT2G47860.5 35.443 395 218 10 112 488 1 376 2.74e-56 199
Msa0079040 AT2G47860.2 35.443 395 218 10 112 488 1 376 2.74e-56 199
Msa0079040 AT1G52770.1 37.288 295 147 6 213 488 28 303 1.95e-54 192
Msa0079040 AT5G64330.3 40.693 231 122 2 40 256 6 235 1.61e-53 193
Msa0079040 AT5G64330.3 37.959 245 132 5 271 499 314 554 1.38e-43 165
Msa0079040 AT5G48130.1 25.407 492 274 13 27 487 39 468 1.31e-52 191
Msa0079040 AT3G15570.1 34.783 299 153 7 213 488 29 308 3.46e-47 172
Msa0079040 AT3G19850.1 27.460 437 275 8 69 487 52 464 1.17e-46 173
Msa0079040 AT5G17580.2 27.484 473 305 10 23 488 16 457 1.21e-41 159
Msa0079040 AT5G17580.1 27.484 473 305 10 23 488 1 442 1.70e-41 158
Msa0079040 AT1G50280.2 27.804 428 277 8 69 482 18 427 1.14e-39 152
Msa0079040 AT1G50280.1 27.804 428 277 8 69 482 51 460 1.41e-39 152
Msa0079040 AT3G03510.1 29.762 420 225 15 77 488 4 361 1.31e-36 142
Msa0079040 AT3G22104.1 24.000 450 287 11 36 473 14 420 6.10e-32 130
Msa0079040 AT3G22104.4 23.117 385 243 9 99 473 4 345 3.12e-22 100
Msa0079040 AT3G22104.2 23.117 385 243 9 99 473 4 345 3.12e-22 100
Msa0079040 AT3G22104.3 23.117 385 243 9 99 473 4 345 3.12e-22 100

Find 150 sgRNAs with CRISPR-Local

Find 292 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGAATTACATAGAATTAAT+TGG 0.181392 1_2:-56727599 Msa0079040:three_prime_UTR
ATTCCTCGTCTCCATTTATT+AGG 0.216595 1_2:+56727935 None:intergenic
TCTGAGGATCTAAAGATAAT+TGG 0.223450 1_2:-56729344 Msa0079040:CDS
CAAAAGACATTCTATCTCTT+AGG 0.246837 1_2:-56727620 Msa0079040:exon
CCTCCACATGCCCTAATAAA+TGG 0.253864 1_2:-56727946 Msa0079040:CDS
ATGTAATGTAATTTGAATTA+AGG 0.275791 1_2:-56727500 Msa0079040:three_prime_UTR
GATATGAAAGATAAAGTATT+TGG 0.280957 1_2:-56728699 Msa0079040:CDS
TGTCAAGGATTTACTGATTC+CGG 0.288586 1_2:-56728823 Msa0079040:CDS
CTGATTCCGGCTAGGTCTCC+TGG 0.293524 1_2:-56728810 Msa0079040:CDS
AATGATAACGGTGAAATCTA+AGG 0.298241 1_2:-56729120 Msa0079040:CDS
TGCACATATTTCAAAGGCCT+TGG 0.314739 1_2:+56729571 None:intergenic
GGAGAATATTTGACAAGTTA+TGG 0.336159 1_2:-56727759 Msa0079040:CDS
TACTACTTATGATATTGATT+TGG 0.339422 1_2:-56728784 Msa0079040:CDS
CTGTACAAACCGTCATGATT+TGG 0.348424 1_2:+56728537 None:intergenic
CGTTGCAGAGAAAGGGTGTT+TGG 0.349618 1_2:-56732464 Msa0079040:five_prime_UTR
ACTATTTAAAGTGGCCATAC+TGG 0.349780 1_2:-56728913 Msa0079040:CDS
CTTGGAGATACAAAATCTTC+TGG 0.359835 1_2:-56727658 Msa0079040:CDS
AATAGGAGTTCAGAGGCTTC+AGG 0.361947 1_2:-56727709 Msa0079040:CDS
TCCAAGATGAGTTAAGATTA+TGG 0.362037 1_2:+56732348 None:intergenic
TTTGTTTCTTTCTGTCTAGT+AGG 0.364013 1_2:-56727421 Msa0079040:three_prime_UTR
CCATCAGATTGAAGTGCATC+AGG 0.365409 1_2:+56732017 None:intergenic
TTCCTCGTCTCCATTTATTA+GGG 0.365614 1_2:+56727936 None:intergenic
GCTGTTCATCAATTCTTCTC+TGG 0.372100 1_2:+56728872 None:intergenic
AAAATGAAGTTTATGAAGTT+GGG 0.381879 1_2:-56732047 Msa0079040:CDS
ACCATAATCTTAACTCATCT+TGG 0.388470 1_2:-56732349 Msa0079040:five_prime_UTR
ATGACAGAGGATATTGATAA+AGG 0.389164 1_2:-56729470 Msa0079040:CDS
GCCTGATTCTGTTGATGCAT+TGG 0.391893 1_2:-56729039 Msa0079040:CDS
ACCAATGCATCAACAGAATC+AGG 0.394719 1_2:+56729038 None:intergenic
AATCTGATGGCAAATTTGTC+AGG 0.409140 1_2:-56732004 Msa0079040:intron
TTGTTTCTTTCTGTCTAGTA+GGG 0.411085 1_2:-56727420 Msa0079040:three_prime_UTR
GTTTCGTAAGAGTGTGAGAT+TGG 0.412767 1_2:-56727839 Msa0079040:CDS
CTGAGGATCTAAAGATAATT+GGG 0.417356 1_2:-56729343 Msa0079040:CDS
TGATTTGGTCTAGCAAAATC+TGG 0.421555 1_2:+56728552 None:intergenic
TTATGGAATGTTGGTAGTAA+AGG 0.422184 1_2:-56727742 Msa0079040:CDS
ACAGATATTATCATAGCTAT+TGG 0.425143 1_2:-56729805 Msa0079040:CDS
TATGGAATGTTGGTAGTAAA+GGG 0.426699 1_2:-56727741 Msa0079040:CDS
TAAGACCAAGTGATGTTTGC+CGG 0.434445 1_2:+56729281 None:intergenic
TTCTTCTCTGGATGATTCAT+CGG 0.435084 1_2:+56728884 None:intergenic
CATCAAGTATCTTTCGTAGC+TGG 0.435226 1_2:-56729415 Msa0079040:CDS
ATCTTAACTCATCTTGGACT+TGG 0.439734 1_2:-56732343 Msa0079040:five_prime_UTR
GATGAGTTAAGATTATGGTT+GGG 0.442387 1_2:+56732353 None:intergenic
CGAGGAATGCGAGAAAACTC+TGG 0.444156 1_2:-56727920 Msa0079040:CDS
GCACATATTTCAAAGGCCTT+GGG 0.445735 1_2:+56729572 None:intergenic
ATGATTAACGTTGCAGAGAA+AGG 0.448086 1_2:-56732472 Msa0079040:five_prime_UTR
TAATTCAGATGAAATTTACT+TGG 0.448094 1_2:-56729612 Msa0079040:CDS
ACGACTTGTTTGTCTGTTGA+GGG 0.448196 1_2:+56727882 None:intergenic
AAAAGAGAGAAAACGTGTTA+AGG 0.451185 1_2:+56732211 None:intergenic
TTCATCGGCTTCAACCAGTA+TGG 0.453351 1_2:+56728899 None:intergenic
GCATGCAGTTGTTGCCGTCT+CGG 0.453505 1_2:-56727786 Msa0079040:CDS
AAGTTGGTTGATAGTTATCT+TGG 0.460673 1_2:-56728636 Msa0079040:CDS
CTTGCTGAAACTATTTAAAG+TGG 0.463625 1_2:-56728922 Msa0079040:CDS
TCCGAAGGATTGGTGGGTTG+AGG 0.463763 1_2:-56729180 Msa0079040:CDS
AAAAGACATTCTATCTCTTA+GGG 0.466446 1_2:-56727619 Msa0079040:exon
TTTGACAAGTTATGGAATGT+TGG 0.466928 1_2:-56727751 Msa0079040:CDS
TCCTCAACCCACCAATCCTT+CGG 0.471360 1_2:+56729179 None:intergenic
TACGAAAGATACTTGATGTA+AGG 0.472293 1_2:+56729420 None:intergenic
AACGACTTGTTTGTCTGTTG+AGG 0.472812 1_2:+56727881 None:intergenic
ATAGGAGTTCAGAGGCTTCA+GGG 0.474284 1_2:-56727708 Msa0079040:CDS
GTTAGCAAATGCTCGGGCAC+TGG 0.475056 1_2:+56727981 None:intergenic
TTGAGTCTGTTCCGAAGGAT+TGG 0.477314 1_2:-56729190 Msa0079040:CDS
CTGCTAAATCTCTTCTCCCT+TGG 0.477644 1_2:-56729367 Msa0079040:CDS
GTAAAGGGCATGGAGAGAAT+AGG 0.477954 1_2:-56727726 Msa0079040:CDS
TGTTTATTGCCGTGTGATAA+CGG 0.480390 1_2:-56728966 Msa0079040:CDS
GAGCAATCGCTTACAGAAGT+TGG 0.481628 1_2:-56729654 Msa0079040:CDS
TCAAAGGCCTTGGGTCCACC+AGG 0.488895 1_2:+56729581 None:intergenic
AGTCCAACTTCTGTAGTCCT+TGG 0.489120 1_2:-56727676 Msa0079040:CDS
TTATAAACGCGTAATGATAA+CGG 0.489211 1_2:-56729132 Msa0079040:CDS
TGCATGGAGGAACAAATCTC+TGG 0.493898 1_2:-56729003 Msa0079040:CDS
TGAAAACCTATGCTGTTAGA+TGG 0.494881 1_2:-56729064 Msa0079040:CDS
ATGAGTTAAGATTATGGTTG+GGG 0.496428 1_2:+56732354 None:intergenic
AAAACGTGTTAAGGAAAGGA+AGG 0.498250 1_2:+56732220 None:intergenic
ATTTACTTGGACGATTTCCC+TGG 0.500638 1_2:-56729599 Msa0079040:CDS
AGATGAGTTAAGATTATGGT+TGG 0.502698 1_2:+56732352 None:intergenic
AGAATGGACGGTGTTGTGAT+TGG 0.502763 1_2:-56729095 Msa0079040:CDS
TGATTCCGGCTAGGTCTCCT+GGG 0.505713 1_2:-56728809 Msa0079040:CDS
GAAATTGACTGCTGATGCAT+CGG 0.507619 1_2:-56728082 Msa0079040:CDS
AGCCATCGATATCTACTTGA+AGG 0.509362 1_2:-56728514 Msa0079040:intron
TCTCCAAGGACTACAGAAGT+TGG 0.509793 1_2:+56727673 None:intergenic
AAACTAGAGAGGCTAAGATT+AGG 0.511940 1_2:+56728597 None:intergenic
AGTCTGTTCCGAAGGATTGG+TGG 0.513817 1_2:-56729187 Msa0079040:CDS
ATCTTTAGATCCTCAGACCA+AGG 0.516375 1_2:+56729350 None:intergenic
TCCACTTCGAGTTGTAGTGC+AGG 0.516877 1_2:-56728034 Msa0079040:CDS
TGGTTTCTGATGCTCATGCA+TGG 0.521841 1_2:-56729019 Msa0079040:CDS
TTGTTTGTCTGTTGAGGGAA+TGG 0.522335 1_2:+56727887 None:intergenic
AGAGAAAACGTGTTAAGGAA+AGG 0.523073 1_2:+56732216 None:intergenic
GGTGAAATCTAAGGGAAGAA+TGG 0.523387 1_2:-56729111 Msa0079040:CDS
AATTAATGTGAAGTGTAAAG+TGG 0.523581 1_2:-56727555 Msa0079040:three_prime_UTR
CTTACAGAAGTTGGTGTCAA+AGG 0.524081 1_2:-56729645 Msa0079040:CDS
TGATTAACGTTGCAGAGAAA+GGG 0.526803 1_2:-56732471 Msa0079040:five_prime_UTR
TTGATCCGGCAAACATCACT+TGG 0.527188 1_2:-56729286 Msa0079040:CDS
CGATTTCCCTGGTGGACCCA+AGG 0.529641 1_2:-56729588 Msa0079040:CDS
ATCTCTTCTCCCTTGGTCTG+AGG 0.535395 1_2:-56729360 Msa0079040:CDS
AGTTGTTGCCGTCTCGGTCG+AGG 0.536571 1_2:-56727780 Msa0079040:CDS
TTGCCGTGTGATAACGGTGT+TGG 0.537879 1_2:-56728960 Msa0079040:CDS
ACCTGCACTACAACTCGAAG+TGG 0.539338 1_2:+56728033 None:intergenic
CCTGATGCACTTCAATCTGA+TGG 0.542206 1_2:-56732017 Msa0079040:CDS
GAACTTTGCACATATTTCAA+AGG 0.543529 1_2:+56729565 None:intergenic
ATATGTGCAAAGTTCTGCTA+TGG 0.544022 1_2:-56729557 Msa0079040:CDS
ATGCAGTTAATAATGTTAGT+TGG 0.546007 1_2:+56732249 None:intergenic
TCTGAAAAGAAGAACATATG+TGG 0.548360 1_2:-56728120 Msa0079040:CDS
GATTGCTCTTTGACAGTAGA+GGG 0.548395 1_2:+56729669 None:intergenic
TACTTGGACGATTTCCCTGG+TGG 0.549505 1_2:-56729596 Msa0079040:CDS
ACCCACGTTTGTTGTGAAGT+AGG 0.550474 1_2:+56732129 None:intergenic
GAAAATTGAGTCTGTTCCGA+AGG 0.556432 1_2:-56729195 Msa0079040:CDS
GATATTGAATCTCGGCAAGT+TGG 0.559092 1_2:-56728652 Msa0079040:CDS
ATGATAACGGTGAAATCTAA+GGG 0.563530 1_2:-56729119 Msa0079040:CDS
GTCTGTTCCGAAGGATTGGT+GGG 0.564671 1_2:-56729186 Msa0079040:CDS
AAGTGAAGAAGAGAGAGTAA+TGG 0.564864 1_2:+56732298 None:intergenic
TCTCCATTTATTAGGGCATG+TGG 0.566345 1_2:+56727943 None:intergenic
GGTTGAGGATATATGTGAGC+TGG 0.567054 1_2:-56729165 Msa0079040:CDS
CAATATCCTCTGTCATCTCA+AGG 0.567324 1_2:+56729477 None:intergenic
ATTGCATGAAGCTTCTGTCA+AGG 0.573895 1_2:-56728838 Msa0079040:CDS
AGGATTTACTGATTCCGGCT+AGG 0.574506 1_2:-56728818 Msa0079040:CDS
AAGTATCTTTCGTAGCTGGA+AGG 0.582873 1_2:-56729411 Msa0079040:CDS
GTGGACTGTTAGCAAATGCT+CGG 0.585051 1_2:+56727974 None:intergenic
AATATTCTCCTCGACCGAGA+CGG 0.585861 1_2:+56727772 None:intergenic
TAGTTTCAGCAAGAAACTAC+AGG 0.589035 1_2:+56728932 None:intergenic
AAGTAAGTGTGAGATAGTTG+AGG 0.589047 1_2:+56732163 None:intergenic
AGGTCTGTACTCTGAGTCTG+TGG 0.594537 1_2:+56732404 None:intergenic
CGATTGCTCTTTGACAGTAG+AGG 0.595990 1_2:+56729668 None:intergenic
GAGGAATGCGAGAAAACTCT+GGG 0.599575 1_2:-56727919 Msa0079040:CDS
TCTTTAGATCCTCAGACCAA+GGG 0.600214 1_2:+56729351 None:intergenic
GTAATCCCAGGAGACCTAGC+CGG 0.600747 1_2:+56728804 None:intergenic
CCATTTATTAGGGCATGTGG+AGG 0.601063 1_2:+56727946 None:intergenic
TCTGAATGCTTACAATGTTG+TGG 0.606892 1_2:-56729522 Msa0079040:CDS
TGGACTGTTAGCAAATGCTC+GGG 0.608053 1_2:+56727975 None:intergenic
AATGTTGGTAGTAAAGGGCA+TGG 0.608411 1_2:-56727736 Msa0079040:CDS
CTCAAAAGAGTGAATGAGAG+AGG 0.609313 1_2:+56732384 None:intergenic
GTCCTACTTCACAACAAACG+TGG 0.610602 1_2:-56732131 Msa0079040:five_prime_UTR
AATAGGGCGATATTGAATCT+CGG 0.610706 1_2:-56728660 Msa0079040:CDS
TACCTTCAAGTAGATATCGA+TGG 0.617470 1_2:+56728512 None:intergenic
ATATCATAAGTAGTAATCCC+AGG 0.618972 1_2:+56728792 None:intergenic
AGGCAACCATCTAACAGCAT+AGG 0.619442 1_2:+56729058 None:intergenic
ACAAATCAACAAAACTAGAG+AGG 0.619836 1_2:+56728586 None:intergenic
TGGAGAGAATAGGAGTTCAG+AGG 0.626635 1_2:-56727716 Msa0079040:CDS
CAACCAACACCGTTATCACA+CGG 0.627146 1_2:+56728957 None:intergenic
TTTGCTAGACCAAATCATGA+CGG 0.635280 1_2:-56728546 Msa0079040:CDS
AAATCTAAGGGAAGAATGGA+CGG 0.638803 1_2:-56729107 Msa0079040:CDS
AATGTGAAGTGTAAAGTGGT+AGG 0.643484 1_2:-56727551 Msa0079040:three_prime_UTR
TGTGGCTGCTCGATGTGCAG+CGG 0.654801 1_2:-56729504 Msa0079040:CDS
ACAGGTCGATAAAGTCGTTG+AGG 0.658797 1_2:-56729237 Msa0079040:CDS
AGGGCATGTGGAGGATTGTG+TGG 0.662550 1_2:+56727955 None:intergenic
TCCTACTTCACAACAAACGT+GGG 0.675999 1_2:-56732130 Msa0079040:five_prime_UTR
TGTAAAGTTTGACCTTCACA+AGG 0.678188 1_2:-56729779 Msa0079040:intron
TGCCCTAATAAATGGAGACG+AGG 0.682274 1_2:-56727938 Msa0079040:CDS
CAAAGGCCTTGGGTCCACCA+GGG 0.683009 1_2:+56729582 None:intergenic
GGACGGTGTTGTGATTGGTG+AGG 0.691521 1_2:-56729090 Msa0079040:CDS
TTACAACCGAAAATTAACAC+AGG 0.700722 1_2:-56729255 Msa0079040:CDS
GGAGTACCTTGAGATGACAG+AGG 0.738740 1_2:-56729483 Msa0079040:CDS
TTTCTGATGCTCATGCATGG+AGG 0.746693 1_2:-56729016 Msa0079040:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGATTATTTCATAAATATTA+TGG - chr1_2:56729089-56729108 Msa0079040:CDS 10.0%
!! TATTAACATAAAAAAATGAT+TGG + chr1_2:56729444-56729463 None:intergenic 10.0%
!!! AATATAAACTGAAATAGTTT+TGG - chr1_2:56728121-56728140 Msa0079040:CDS 15.0%
!! TATGTTATTATTAACTGTAA+AGG - chr1_2:56728763-56728782 Msa0079040:CDS 15.0%
!! ATGTTATTATTAACTGTAAA+GGG - chr1_2:56728764-56728783 Msa0079040:CDS 15.0%
!! GTAAAACTAATGATATATAT+AGG + chr1_2:56729006-56729025 None:intergenic 15.0%
!! TATTTCATAAATATTATGGT+TGG - chr1_2:56729093-56729112 Msa0079040:CDS 15.0%
!!! CTTGATTTTTTAACTTAATT+TGG + chr1_2:56729209-56729228 None:intergenic 15.0%
!! AATTAAGTTAAAAAATCAAG+AGG - chr1_2:56729209-56729228 Msa0079040:CDS 15.0%
!! ATTAAGTTAAAAAATCAAGA+GGG - chr1_2:56729210-56729229 Msa0079040:CDS 15.0%
!! TTAATATCAACAATTATTAG+TGG + chr1_2:56729303-56729322 None:intergenic 15.0%
!!! TGCAACTATTTTATTAAATA+AGG + chr1_2:56729499-56729518 None:intergenic 15.0%
!!! AGTTTTATTAAAAAATGCAA+TGG - chr1_2:56729811-56729830 Msa0079040:CDS 15.0%
!!! TCTTTTTCTGTTATATTTTT+TGG - chr1_2:56731443-56731462 Msa0079040:intron 15.0%
!! ATGTAATGTAATTTGAATTA+AGG - chr1_2:56732402-56732421 Msa0079040:five_prime_UTR 15.0%
!! AAAAATGAAGTTTATGAAGT+TGG - chr1_2:56727854-56727873 Msa0079040:CDS 20.0%
!! AAAATGAAGTTTATGAAGTT+GGG - chr1_2:56727855-56727874 Msa0079040:CDS 20.0%
!!! TGATTTTCTCAAATTATTAG+CGG - chr1_2:56728559-56728578 Msa0079040:CDS 20.0%
!! ATATATTGAAACTATTTCAG+TGG + chr1_2:56728713-56728732 None:intergenic 20.0%
!! GTAGTGTACTATTAAATTAT+TGG - chr1_2:56729172-56729191 Msa0079040:CDS 20.0%
!!! GATTTTTTAACTTAATTTGG+AGG + chr1_2:56729206-56729225 None:intergenic 20.0%
!!! ACTTTAAAAAGCTAGTATTT+TGG - chr1_2:56729332-56729351 Msa0079040:CDS 20.0%
!!! TATTACATTTTTCTATCACT+AGG + chr1_2:56729792-56729811 None:intergenic 20.0%
!! TAATTCAGATGAAATTTACT+TGG - chr1_2:56730290-56730309 Msa0079040:intron 20.0%
!!! TACTACTTATGATATTGATT+TGG - chr1_2:56731118-56731137 Msa0079040:intron 20.0%
!!! GATATGAAAGATAAAGTATT+TGG - chr1_2:56731203-56731222 Msa0079040:intron 20.0%
!!! TATTTGGTGAATCTATTTTA+GGG - chr1_2:56731219-56731238 Msa0079040:intron 20.0%
!!! TTATTTTCTCGAATTATTTC+AGG + chr1_2:56731524-56731543 None:intergenic 20.0%
! ATGCAGTTAATAATGTTAGT+TGG + chr1_2:56727656-56727675 None:intergenic 25.0%
! AAGTTTGTGACAAAAAAACA+AGG + chr1_2:56727802-56727821 None:intergenic 25.0%
! CTAAAGAAACAAGAAAGTAA+AGG + chr1_2:56727984-56728003 None:intergenic 25.0%
!! TTTTTCTTTTTCTGTACTGT+TGG - chr1_2:56728031-56728050 Msa0079040:CDS 25.0%
! AAAAAGATTCAAATGGTTAC+TGG + chr1_2:56728164-56728183 None:intergenic 25.0%
! GCAACATAAAAAGATTCAAA+TGG + chr1_2:56728171-56728190 None:intergenic 25.0%
!! TTTGTTATGACAGTAAATCA+CGG - chr1_2:56728194-56728213 Msa0079040:intron 25.0%
!! AGTTTCTGGAAAATAAATCA+CGG + chr1_2:56728227-56728246 None:intergenic 25.0%
!!! TTTATTTTCCAGAAACTTTG+AGG - chr1_2:56728230-56728249 Msa0079040:intron 25.0%
! ACATTGCTTTGTATATGTTT+CGG + chr1_2:56728291-56728310 None:intergenic 25.0%
!!! AATGTTTTAAAAGCCAAACA+TGG - chr1_2:56728306-56728325 Msa0079040:intron 25.0%
! TGAGAAAATCAAGTAATTCA+AGG + chr1_2:56728550-56728569 None:intergenic 25.0%
! TTGCATACAATGAGTTATAT+GGG + chr1_2:56728686-56728705 None:intergenic 25.0%
! TTTGCATACAATGAGTTATA+TGG + chr1_2:56728687-56728706 None:intergenic 25.0%
!!! ATAGTTTGCAAAGTCTTTTA+TGG - chr1_2:56728926-56728945 Msa0079040:CDS 25.0%
! AAATAGTTGCAAGTATATGA+TGG - chr1_2:56729508-56729527 Msa0079040:CDS 25.0%
! TAACTTATACAAGGAAAGAT+GGG + chr1_2:56730016-56730035 None:intergenic 25.0%
! TACAAAGTGTAACTTATACA+AGG + chr1_2:56730025-56730044 None:intergenic 25.0%
! AATTCATTTCCAATTTATGC+AGG - chr1_2:56730053-56730072 Msa0079040:intron 25.0%
! ACAGATATTATCATAGCTAT+TGG - chr1_2:56730097-56730116 Msa0079040:intron 25.0%
! TTTAACAGGAAACTAATACA+AGG + chr1_2:56730167-56730186 None:intergenic 25.0%
! AGAAGAATTGCTTATTTAAC+AGG + chr1_2:56730181-56730200 None:intergenic 25.0%
! ACATGAGAAGTAAAAATCAA+TGG + chr1_2:56730204-56730223 None:intergenic 25.0%
! TTATAAACGCGTAATGATAA+CGG - chr1_2:56730770-56730789 Msa0079040:intron 25.0%
!!! GTATTTGGTGAATCTATTTT+AGG - chr1_2:56731218-56731237 Msa0079040:intron 25.0%
!!! TGAATCTATTTTAGGGAATA+GGG - chr1_2:56731226-56731245 Msa0079040:intron 25.0%
!! TATTTTGTTAGTGTCATGTT+AGG + chr1_2:56731498-56731517 None:intergenic 25.0%
!!! TGTTCTAAAATGTGATATAC+TGG + chr1_2:56731653-56731672 None:intergenic 25.0%
! TATTAAGACAAAGAACAGAT+TGG + chr1_2:56731698-56731717 None:intergenic 25.0%
!! TTTCAGATTTTGTCAAACTT+GGG + chr1_2:56731769-56731788 None:intergenic 25.0%
!! TTTTCAGATTTTGTCAAACT+TGG + chr1_2:56731770-56731789 None:intergenic 25.0%
! AAAAGACATTCTATCTCTTA+GGG - chr1_2:56732283-56732302 Msa0079040:five_prime_UTR 25.0%
! GGGAATTACATAGAATTAAT+TGG - chr1_2:56732303-56732322 Msa0079040:five_prime_UTR 25.0%
! AATTAATGTGAAGTGTAAAG+TGG - chr1_2:56732347-56732366 Msa0079040:five_prime_UTR 25.0%
ATGAGTTAAGATTATGGTTG+GGG + chr1_2:56727551-56727570 None:intergenic 30.0%
GATGAGTTAAGATTATGGTT+GGG + chr1_2:56727552-56727571 None:intergenic 30.0%
AGATGAGTTAAGATTATGGT+TGG + chr1_2:56727553-56727572 None:intergenic 30.0%
ACCATAATCTTAACTCATCT+TGG - chr1_2:56727553-56727572 Msa0079040:three_prime_UTR 30.0%
TCCAAGATGAGTTAAGATTA+TGG + chr1_2:56727557-56727576 None:intergenic 30.0%
!!! GACTTGGTTTTAAGCAAATA+AGG - chr1_2:56727575-56727594 Msa0079040:three_prime_UTR 30.0%
!!! TTGGTTTTAAGCAAATAAGG+CGG - chr1_2:56727578-56727597 Msa0079040:three_prime_UTR 30.0%
AAAAGAGAGAAAACGTGTTA+AGG + chr1_2:56727694-56727713 None:intergenic 30.0%
TGTCACAAACTTTCTTTGTT+TGG - chr1_2:56727810-56727829 Msa0079040:CDS 30.0%
!!! ACTGAAATAGTTTTGGTGTT+TGG - chr1_2:56728128-56728147 Msa0079040:CDS 30.0%
! GTGATTTTGCAAAAGCTATA+AGG - chr1_2:56728365-56728384 Msa0079040:intron 30.0%
! GTAATTATATGTGTCGATGT+TGG + chr1_2:56728528-56728547 None:intergenic 30.0%
! TGTAGCACATGATTTTTAAC+AGG + chr1_2:56729129-56729148 None:intergenic 30.0%
! ATTTGATCAAGTTTTGCGTT+TGG - chr1_2:56729655-56729674 Msa0079040:CDS 30.0%
GAATCAAACTCAATACTTCA+GGG + chr1_2:56729770-56729789 None:intergenic 30.0%
! ACATGCATCTTAATACCTTT+TGG - chr1_2:56729877-56729896 Msa0079040:intron 30.0%
AACTTATACAAGGAAAGATG+GGG + chr1_2:56730015-56730034 None:intergenic 30.0%
GTAACTTATACAAGGAAAGA+TGG + chr1_2:56730017-56730036 None:intergenic 30.0%
TGATATGTACCTGCATAAAT+TGG + chr1_2:56730065-56730084 None:intergenic 30.0%
GAACTTTGCACATATTTCAA+AGG + chr1_2:56730340-56730359 None:intergenic 30.0%
ATGACAGAGGATATTGATAA+AGG - chr1_2:56730432-56730451 Msa0079040:intron 30.0%
TACGAAAGATACTTGATGTA+AGG + chr1_2:56730485-56730504 None:intergenic 30.0%
TCTGAGGATCTAAAGATAAT+TGG - chr1_2:56730558-56730577 Msa0079040:intron 30.0%
CTGAGGATCTAAAGATAATT+GGG - chr1_2:56730559-56730578 Msa0079040:intron 30.0%
! AACAGAAGTTTTTGATGCTA+TGG + chr1_2:56730598-56730617 None:intergenic 30.0%
ATCAAAAACTTCTGTTGATC+CGG - chr1_2:56730602-56730621 Msa0079040:intron 30.0%
TTACAACCGAAAATTAACAC+AGG - chr1_2:56730647-56730666 Msa0079040:intron 30.0%
! TATCGACCTGTGTTAATTTT+CGG + chr1_2:56730656-56730675 None:intergenic 30.0%
!! AATGATAACGGTGAAATCTA+AGG - chr1_2:56730782-56730801 Msa0079040:intron 30.0%
!! ATGATAACGGTGAAATCTAA+GGG - chr1_2:56730783-56730802 Msa0079040:intron 30.0%
CTTGCTGAAACTATTTAAAG+TGG - chr1_2:56730980-56730999 Msa0079040:intron 30.0%
ATATCATAAGTAGTAATCCC+AGG + chr1_2:56731113-56731132 None:intergenic 30.0%
! AAAGAAAAAGCGTGATATTG+AGG - chr1_2:56731175-56731194 Msa0079040:intron 30.0%
!! GTGAATCTATTTTAGGGAAT+AGG - chr1_2:56731225-56731244 Msa0079040:intron 30.0%
!! AAGTTGGTTGATAGTTATCT+TGG - chr1_2:56731266-56731285 Msa0079040:intron 30.0%
ACAAATCAACAAAACTAGAG+AGG + chr1_2:56731319-56731338 None:intergenic 30.0%
TCTGAAAAGAAGAACATATG+TGG - chr1_2:56731782-56731801 Msa0079040:intron 30.0%
GGAGAATATTTGACAAGTTA+TGG - chr1_2:56732143-56732162 Msa0079040:five_prime_UTR 30.0%
TTTGACAAGTTATGGAATGT+TGG - chr1_2:56732151-56732170 Msa0079040:five_prime_UTR 30.0%
!! TTATGGAATGTTGGTAGTAA+AGG - chr1_2:56732160-56732179 Msa0079040:five_prime_UTR 30.0%
!! TATGGAATGTTGGTAGTAAA+GGG - chr1_2:56732161-56732180 Msa0079040:five_prime_UTR 30.0%
! AGAATGTCTTTTGTGTCTTA+AGG + chr1_2:56732275-56732294 None:intergenic 30.0%
CAAAAGACATTCTATCTCTT+AGG - chr1_2:56732282-56732301 Msa0079040:five_prime_UTR 30.0%
! TTTGTTTCTTTCTGTCTAGT+AGG - chr1_2:56732481-56732500 Msa0079040:five_prime_UTR 30.0%
ATGATTAACGTTGCAGAGAA+AGG - chr1_2:56727430-56727449 Msa0079040:three_prime_UTR 35.0%
TGATTAACGTTGCAGAGAAA+GGG - chr1_2:56727431-56727450 Msa0079040:three_prime_UTR 35.0%
ATCTTAACTCATCTTGGACT+TGG - chr1_2:56727559-56727578 Msa0079040:three_prime_UTR 35.0%
AAGTGAAGAAGAGAGAGTAA+TGG + chr1_2:56727607-56727626 None:intergenic 35.0%
AAAACGTGTTAAGGAAAGGA+AGG + chr1_2:56727685-56727704 None:intergenic 35.0%
AGAGAAAACGTGTTAAGGAA+AGG + chr1_2:56727689-56727708 None:intergenic 35.0%
AAGTAAGTGTGAGATAGTTG+AGG + chr1_2:56727742-56727761 None:intergenic 35.0%
AATCTGATGGCAAATTTGTC+AGG - chr1_2:56727898-56727917 Msa0079040:CDS 35.0%
!! ATTTTCCAGAAACTTTGAGG+TGG - chr1_2:56728233-56728252 Msa0079040:intron 35.0%
CAATGAGTTTGTGAGAATCA+AGG + chr1_2:56728263-56728282 None:intergenic 35.0%
TCTCAAATTATTAGCGGTGT+CGG - chr1_2:56728565-56728584 Msa0079040:CDS 35.0%
AAGATGCCAGCTATGAAATA+CGG + chr1_2:56728620-56728639 None:intergenic 35.0%
GTTATATGGGTTTCTCTTGA+AGG + chr1_2:56728673-56728692 None:intergenic 35.0%
AGAGAGAGAGGTTTAATTGA+AGG - chr1_2:56728807-56728826 Msa0079040:CDS 35.0%
ACCAGAAACTCACAATTTGA+AGG + chr1_2:56728841-56728860 None:intergenic 35.0%
TCCAAACAGTGTGTATGTAT+AGG - chr1_2:56728872-56728891 Msa0079040:CDS 35.0%
ACCTATACATACACACTGTT+TGG + chr1_2:56728876-56728895 None:intergenic 35.0%
AAACAGTGTGTATGTATAGG+TGG - chr1_2:56728875-56728894 Msa0079040:CDS 35.0%
! TTTTAGCATGTGAGTTTCAC+TGG - chr1_2:56729027-56729046 Msa0079040:CDS 35.0%
CTACACATGTATTCCACATT+TGG - chr1_2:56729144-56729163 Msa0079040:CDS 35.0%
AGACATGCTTAACAAAGCAT+TGG + chr1_2:56729249-56729268 None:intergenic 35.0%
!!! TGCAGTTAGCCATTTTTGTA+GGG - chr1_2:56729362-56729381 Msa0079040:CDS 35.0%
ATTTGCAGACCCTACAAAAA+TGG + chr1_2:56729374-56729393 None:intergenic 35.0%
TACACTAATAGTTTACCACC+AGG - chr1_2:56729702-56729721 Msa0079040:intron 35.0%
ACACTAATAGTTTACCACCA+GGG - chr1_2:56729703-56729722 Msa0079040:intron 35.0%
GGAATCAAACTCAATACTTC+AGG + chr1_2:56729771-56729790 None:intergenic 35.0%
! TGTTTTTCACCACATTCACA+TGG + chr1_2:56729860-56729879 None:intergenic 35.0%
TGTAAAGTTTGACCTTCACA+AGG - chr1_2:56730123-56730142 Msa0079040:intron 35.0%
! AAAAAGTACACACCTTGTGA+AGG + chr1_2:56730138-56730157 None:intergenic 35.0%
ATATGTGCAAAGTTCTGCTA+TGG - chr1_2:56730345-56730364 Msa0079040:intron 35.0%
TCTGAATGCTTACAATGTTG+TGG - chr1_2:56730380-56730399 Msa0079040:intron 35.0%
AAATCTAAGGGAAGAATGGA+CGG - chr1_2:56730795-56730814 Msa0079040:intron 35.0%
TGAAAACCTATGCTGTTAGA+TGG - chr1_2:56730838-56730857 Msa0079040:intron 35.0%
TGTTTATTGCCGTGTGATAA+CGG - chr1_2:56730936-56730955 Msa0079040:intron 35.0%
TAGTTTCAGCAAGAAACTAC+AGG + chr1_2:56730973-56730992 None:intergenic 35.0%
! ACTATTTAAAGTGGCCATAC+TGG - chr1_2:56730989-56731008 Msa0079040:intron 35.0%
TTCTTCTCTGGATGATTCAT+CGG + chr1_2:56731021-56731040 None:intergenic 35.0%
!! TGTCAAGGATTTACTGATTC+CGG - chr1_2:56731079-56731098 Msa0079040:intron 35.0%
AATAGGGCGATATTGAATCT+CGG - chr1_2:56731242-56731261 Msa0079040:intron 35.0%
AAACTAGAGAGGCTAAGATT+AGG + chr1_2:56731308-56731327 None:intergenic 35.0%
!! TGATTTGGTCTAGCAAAATC+TGG + chr1_2:56731353-56731372 None:intergenic 35.0%
TTTGCTAGACCAAATCATGA+CGG - chr1_2:56731356-56731375 Msa0079040:intron 35.0%
TACCTTCAAGTAGATATCGA+TGG + chr1_2:56731393-56731412 None:intergenic 35.0%
! AAGGTAAGATTTTGCTACCT+TGG - chr1_2:56731407-56731426 Msa0079040:intron 35.0%
!!! TTTTTTGGTCTGTGAAGCAT+TGG - chr1_2:56731458-56731477 Msa0079040:intron 35.0%
! CATTTGCGTGAATCATTTTC+AGG - chr1_2:56731739-56731758 Msa0079040:intron 35.0%
TTCCTCGTCTCCATTTATTA+GGG + chr1_2:56731969-56731988 None:intergenic 35.0%
ATTCCTCGTCTCCATTTATT+AGG + chr1_2:56731970-56731989 None:intergenic 35.0%
AGTAGCAAAAATAGCAGAAG+TGG - chr1_2:56732094-56732113 Msa0079040:intron 35.0%
! CAGAAGATTTTGTATCTCCA+AGG + chr1_2:56732246-56732265 None:intergenic 35.0%
CTTGGAGATACAAAATCTTC+TGG - chr1_2:56732244-56732263 Msa0079040:five_prime_UTR 35.0%
AATGTGAAGTGTAAAGTGGT+AGG - chr1_2:56732351-56732370 Msa0079040:five_prime_UTR 35.0%
CTCAAAAGAGTGAATGAGAG+AGG + chr1_2:56727521-56727540 None:intergenic 40.0%
TCCTACTTCACAACAAACGT+GGG - chr1_2:56727772-56727791 Msa0079040:CDS 40.0%
GTAAACCACCTCAAAGTTTC+TGG + chr1_2:56728241-56728260 None:intergenic 40.0%
! GATTAGTCATGTCCCAATGT+CGG - chr1_2:56728496-56728515 Msa0079040:intron 40.0%
CCAGCTATGAAATACGGATA+CGG + chr1_2:56728614-56728633 None:intergenic 40.0%
GAGAGAGGTTTAATTGAAGG+TGG - chr1_2:56728810-56728829 Msa0079040:CDS 40.0%
GAAACTCACAATTTGAAGGC+TGG + chr1_2:56728837-56728856 None:intergenic 40.0%
GCCTTCAAATTGTGAGTTTC+TGG - chr1_2:56728837-56728856 Msa0079040:CDS 40.0%
GTGTGTATGTATAGGTGGTA+TGG - chr1_2:56728880-56728899 Msa0079040:CDS 40.0%
TACACTACACTGACCAAATG+TGG + chr1_2:56729160-56729179 None:intergenic 40.0%
CTACAAAAATGGCTAACTGC+AGG + chr1_2:56729363-56729382 None:intergenic 40.0%
!!! CTGCAGTTAGCCATTTTTGT+AGG - chr1_2:56729361-56729380 Msa0079040:CDS 40.0%
GTGATGTATCCATGTGAATG+TGG - chr1_2:56729848-56729867 Msa0079040:intron 40.0%
GCAACTGAAATTCAGCCAAA+AGG + chr1_2:56729895-56729914 None:intergenic 40.0%
ATTTGCATGCCTACATTGTG+TGG - chr1_2:56729948-56729967 Msa0079040:intron 40.0%
TTTGCATGCCTACATTGTGT+GGG - chr1_2:56729949-56729968 Msa0079040:intron 40.0%
ACAATTACCCCACACAATGT+AGG + chr1_2:56729960-56729979 None:intergenic 40.0%
GATTGCTCTTTGACAGTAGA+GGG + chr1_2:56730236-56730255 None:intergenic 40.0%
!! CTTACAGAAGTTGGTGTCAA+AGG - chr1_2:56730257-56730276 Msa0079040:intron 40.0%
ATTTACTTGGACGATTTCCC+TGG - chr1_2:56730303-56730322 Msa0079040:intron 40.0%
GCACATATTTCAAAGGCCTT+GGG + chr1_2:56730333-56730352 None:intergenic 40.0%
TGCACATATTTCAAAGGCCT+TGG + chr1_2:56730334-56730353 None:intergenic 40.0%
CAATATCCTCTGTCATCTCA+AGG + chr1_2:56730428-56730447 None:intergenic 40.0%
CATCAAGTATCTTTCGTAGC+TGG - chr1_2:56730487-56730506 Msa0079040:intron 40.0%
AAGTATCTTTCGTAGCTGGA+AGG - chr1_2:56730491-56730510 Msa0079040:intron 40.0%
TCTTTAGATCCTCAGACCAA+GGG + chr1_2:56730554-56730573 None:intergenic 40.0%
ATCTTTAGATCCTCAGACCA+AGG + chr1_2:56730555-56730574 None:intergenic 40.0%
TAAGACCAAGTGATGTTTGC+CGG + chr1_2:56730624-56730643 None:intergenic 40.0%
GAAAATTGAGTCTGTTCCGA+AGG - chr1_2:56730707-56730726 Msa0079040:intron 40.0%
GGTGAAATCTAAGGGAAGAA+TGG - chr1_2:56730791-56730810 Msa0079040:intron 40.0%
ACCAATGCATCAACAGAATC+AGG + chr1_2:56730867-56730886 None:intergenic 40.0%
GCTGTTCATCAATTCTTCTC+TGG + chr1_2:56731033-56731052 None:intergenic 40.0%
ATTGCATGAAGCTTCTGTCA+AGG - chr1_2:56731064-56731083 Msa0079040:intron 40.0%
GATATTGAATCTCGGCAAGT+TGG - chr1_2:56731250-56731269 Msa0079040:intron 40.0%
CTGTACAAACCGTCATGATT+TGG + chr1_2:56731368-56731387 None:intergenic 40.0%
AGCCATCGATATCTACTTGA+AGG - chr1_2:56731388-56731407 Msa0079040:intron 40.0%
! GTAAGATTTTGCTACCTTGG+AGG - chr1_2:56731410-56731429 Msa0079040:intron 40.0%
AAGAAGTGAAAACACCTCCA+AGG + chr1_2:56731427-56731446 None:intergenic 40.0%
! ATGTCTTCCACAGAAAGTGT+CGG - chr1_2:56731571-56731590 Msa0079040:intron 40.0%
! TAGTTGTGCAAGCATTGTTC+AGG + chr1_2:56731630-56731649 None:intergenic 40.0%
GAAATTGACTGCTGATGCAT+CGG - chr1_2:56731820-56731839 Msa0079040:intron 40.0%
TCTCCATTTATTAGGGCATG+TGG + chr1_2:56731962-56731981 None:intergenic 40.0%
TTGTTTGTCTGTTGAGGGAA+TGG + chr1_2:56732018-56732037 None:intergenic 40.0%
! ACGACTTGTTTGTCTGTTGA+GGG + chr1_2:56732023-56732042 None:intergenic 40.0%
! AACGACTTGTTTGTCTGTTG+AGG + chr1_2:56732024-56732043 None:intergenic 40.0%
! GTTTCGTAAGAGTGTGAGAT+TGG - chr1_2:56732063-56732082 Msa0079040:exon 40.0%
!! AATGTTGGTAGTAAAGGGCA+TGG - chr1_2:56732166-56732185 Msa0079040:five_prime_UTR 40.0%
GTCCTACTTCACAACAAACG+TGG - chr1_2:56727771-56727790 Msa0079040:CDS 45.0%
!! ACCCACGTTTGTTGTGAAGT+AGG + chr1_2:56727776-56727795 None:intergenic 45.0%
CCATCAGATTGAAGTGCATC+AGG + chr1_2:56727888-56727907 None:intergenic 45.0%
CCTGATGCACTTCAATCTGA+TGG - chr1_2:56727885-56727904 Msa0079040:CDS 45.0%
GATAGGATGTCTGCTAGGTT+CGG + chr1_2:56728457-56728476 None:intergenic 45.0%
!! AGGAGTGTTTGTGGTTCGAT+AGG + chr1_2:56728474-56728493 None:intergenic 45.0%
ATCGAACCACAAACACTCCT+CGG - chr1_2:56728474-56728493 Msa0079040:intron 45.0%
!! ACTAATCCGAGGAGTGTTTG+TGG + chr1_2:56728483-56728502 None:intergenic 45.0%
ATTGGGACATGACTAATCCG+AGG + chr1_2:56728494-56728513 None:intergenic 45.0%
TGAAATACGGATACGGACAC+CGG + chr1_2:56728607-56728626 None:intergenic 45.0%
CCGTATCCGTATTTCATAGC+TGG - chr1_2:56728611-56728630 Msa0079040:CDS 45.0%
!! TGAGTTTCTGGTTGAGCTTG+TGG - chr1_2:56728849-56728868 Msa0079040:CDS 45.0%
CAACTACTTTCACTACACCC+TGG + chr1_2:56729723-56729742 None:intergenic 45.0%
CAGGGTGTAGTGAAAGTAGT+TGG - chr1_2:56729721-56729740 Msa0079040:intron 45.0%
TTGCATGCCTACATTGTGTG+GGG - chr1_2:56729950-56729969 Msa0079040:intron 45.0%
CGATTGCTCTTTGACAGTAG+AGG + chr1_2:56730237-56730256 None:intergenic 45.0%
GAGCAATCGCTTACAGAAGT+TGG - chr1_2:56730248-56730267 Msa0079040:intron 45.0%
CTGCTAAATCTCTTCTCCCT+TGG - chr1_2:56730535-56730554 Msa0079040:intron 45.0%
TTGATCCGGCAAACATCACT+TGG - chr1_2:56730616-56730635 Msa0079040:intron 45.0%
ACAGGTCGATAAAGTCGTTG+AGG - chr1_2:56730665-56730684 Msa0079040:intron 45.0%
TTGAGTCTGTTCCGAAGGAT+TGG - chr1_2:56730712-56730731 Msa0079040:intron 45.0%
GGTTGAGGATATATGTGAGC+TGG - chr1_2:56730737-56730756 Msa0079040:intron 45.0%
!! AGAATGGACGGTGTTGTGAT+TGG - chr1_2:56730807-56730826 Msa0079040:intron 45.0%
AGGCAACCATCTAACAGCAT+AGG + chr1_2:56730847-56730866 None:intergenic 45.0%
GCCTGATTCTGTTGATGCAT+TGG - chr1_2:56730863-56730882 Msa0079040:intron 45.0%
TGGTTTCTGATGCTCATGCA+TGG - chr1_2:56730883-56730902 Msa0079040:intron 45.0%
TTTCTGATGCTCATGCATGG+AGG - chr1_2:56730886-56730905 Msa0079040:intron 45.0%
TGCATGGAGGAACAAATCTC+TGG - chr1_2:56730899-56730918 Msa0079040:intron 45.0%
CAACCAACACCGTTATCACA+CGG + chr1_2:56730948-56730967 None:intergenic 45.0%
!! TTCATCGGCTTCAACCAGTA+TGG + chr1_2:56731006-56731025 None:intergenic 45.0%
!! AGGATTTACTGATTCCGGCT+AGG - chr1_2:56731084-56731103 Msa0079040:intron 45.0%
!! TATAGCACCGACACTTTCTG+TGG + chr1_2:56731581-56731600 None:intergenic 45.0%
TGGACTGTTAGCAAATGCTC+GGG + chr1_2:56731930-56731949 None:intergenic 45.0%
! GTGGACTGTTAGCAAATGCT+CGG + chr1_2:56731931-56731950 None:intergenic 45.0%
CCATTTATTAGGGCATGTGG+AGG + chr1_2:56731959-56731978 None:intergenic 45.0%
CCTCCACATGCCCTAATAAA+TGG - chr1_2:56731956-56731975 Msa0079040:intron 45.0%
TGCCCTAATAAATGGAGACG+AGG - chr1_2:56731964-56731983 Msa0079040:intron 45.0%
GAGGAATGCGAGAAAACTCT+GGG - chr1_2:56731983-56732002 Msa0079040:intron 45.0%
AATATTCTCCTCGACCGAGA+CGG + chr1_2:56732133-56732152 None:intergenic 45.0%
! GTAAAGGGCATGGAGAGAAT+AGG - chr1_2:56732176-56732195 Msa0079040:five_prime_UTR 45.0%
TGGAGAGAATAGGAGTTCAG+AGG - chr1_2:56732186-56732205 Msa0079040:five_prime_UTR 45.0%
AATAGGAGTTCAGAGGCTTC+AGG - chr1_2:56732193-56732212 Msa0079040:five_prime_UTR 45.0%
ATAGGAGTTCAGAGGCTTCA+GGG - chr1_2:56732194-56732213 Msa0079040:five_prime_UTR 45.0%
AGTCCAACTTCTGTAGTCCT+TGG - chr1_2:56732226-56732245 Msa0079040:five_prime_UTR 45.0%
TCTCCAAGGACTACAGAAGT+TGG + chr1_2:56732232-56732251 None:intergenic 45.0%
!! CGTTGCAGAGAAAGGGTGTT+TGG - chr1_2:56727438-56727457 Msa0079040:three_prime_UTR 50.0%
AGGTCTGTACTCTGAGTCTG+TGG + chr1_2:56727501-56727520 None:intergenic 50.0%
ACCTAACGCGGTACCATGTT+TGG + chr1_2:56728322-56728341 None:intergenic 50.0%
GGTTCGATAGGATGTCTGCT+AGG + chr1_2:56728462-56728481 None:intergenic 50.0%
TGTTGGACACGACCGACATT+GGG + chr1_2:56728511-56728530 None:intergenic 50.0%
ATGTTGGACACGACCGACAT+TGG + chr1_2:56728512-56728531 None:intergenic 50.0%
TCTGAGAGAGAGAGAGAGAG+AGG - chr1_2:56728795-56728814 Msa0079040:CDS 50.0%
CTACTTTCACTACACCCTGG+TGG + chr1_2:56729720-56729739 None:intergenic 50.0%
! GTGAAAGTAGTTGGTGCGCA+TGG - chr1_2:56729730-56729749 Msa0079040:intron 50.0%
! TAGTTGGTGCGCATGGTATG+TGG - chr1_2:56729737-56729756 Msa0079040:intron 50.0%
! AGTTGGTGCGCATGGTATGT+GGG - chr1_2:56729738-56729757 Msa0079040:intron 50.0%
TACTTGGACGATTTCCCTGG+TGG - chr1_2:56730306-56730325 Msa0079040:intron 50.0%
! GGAGTACCTTGAGATGACAG+AGG - chr1_2:56730419-56730438 Msa0079040:intron 50.0%
ATCTCTTCTCCCTTGGTCTG+AGG - chr1_2:56730542-56730561 Msa0079040:intron 50.0%
AGTCTGTTCCGAAGGATTGG+TGG - chr1_2:56730715-56730734 Msa0079040:intron 50.0%
! GTCTGTTCCGAAGGATTGGT+GGG - chr1_2:56730716-56730735 Msa0079040:intron 50.0%
TCCTCAACCCACCAATCCTT+CGG + chr1_2:56730726-56730745 None:intergenic 50.0%
!! TTGCCGTGTGATAACGGTGT+TGG - chr1_2:56730942-56730961 Msa0079040:intron 50.0%
!! TCCACTTCGAGTTGTAGTGC+AGG - chr1_2:56731868-56731887 Msa0079040:intron 50.0%
ACCTGCACTACAACTCGAAG+TGG + chr1_2:56731872-56731891 None:intergenic 50.0%
CGAGGAATGCGAGAAAACTC+TGG - chr1_2:56731982-56732001 Msa0079040:intron 50.0%
GCCAAACATGGTACCGCGTT+AGG - chr1_2:56728318-56728337 Msa0079040:intron 55.0%
! ATGGTACCGCGTTAGGTTGC+CGG - chr1_2:56728325-56728344 Msa0079040:intron 55.0%
GTGATTCCGGCAACCTAACG+CGG + chr1_2:56728334-56728353 None:intergenic 55.0%
! GCGTTAGGTTGCCGGAATCA+CGG - chr1_2:56728333-56728352 Msa0079040:intron 55.0%
! TTAGGTTGCCGGAATCACGG+TGG - chr1_2:56728336-56728355 Msa0079040:intron 55.0%
ACTGCTAGCCACCGTGATTC+CGG + chr1_2:56728347-56728366 None:intergenic 55.0%
GTTGGTGCGCATGGTATGTG+GGG - chr1_2:56729739-56729758 Msa0079040:intron 55.0%
!! TCCGAAGGATTGGTGGGTTG+AGG - chr1_2:56730722-56730741 Msa0079040:intron 55.0%
!! GGACGGTGTTGTGATTGGTG+AGG - chr1_2:56730812-56730831 Msa0079040:intron 55.0%
TGATTCCGGCTAGGTCTCCT+GGG - chr1_2:56731093-56731112 Msa0079040:intron 55.0%
GTAATCCCAGGAGACCTAGC+CGG + chr1_2:56731101-56731120 None:intergenic 55.0%
GTTAGCAAATGCTCGGGCAC+TGG + chr1_2:56731924-56731943 None:intergenic 55.0%
AGGGCATGTGGAGGATTGTG+TGG + chr1_2:56731950-56731969 None:intergenic 55.0%
GCATGCAGTTGTTGCCGTCT+CGG - chr1_2:56732116-56732135 Msa0079040:intron 55.0%
! CGATTTCCCTGGTGGACCCA+AGG - chr1_2:56730314-56730333 Msa0079040:intron 60.0%
CAAAGGCCTTGGGTCCACCA+GGG + chr1_2:56730323-56730342 None:intergenic 60.0%
TCAAAGGCCTTGGGTCCACC+AGG + chr1_2:56730324-56730343 None:intergenic 60.0%
TGTGGCTGCTCGATGTGCAG+CGG - chr1_2:56730398-56730417 Msa0079040:intron 60.0%
CTGATTCCGGCTAGGTCTCC+TGG - chr1_2:56731092-56731111 Msa0079040:intron 60.0%
AGTTGTTGCCGTCTCGGTCG+AGG - chr1_2:56732122-56732141 Msa0079040:five_prime_UTR 60.0%
CGGCGTCTCAGTGTCGTCTC+CGG - chr1_2:56728585-56728604 Msa0079040:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 56727420 56732504 56727420 ID=Msa0079040;Name=Msa0079040
chr1_2 mRNA 56727420 56732504 56727420 ID=Msa0079040-mRNA-1;Parent=Msa0079040;Name=Msa0079040-mRNA-1;_AED=0.02;_eAED=0.02;_QI=400|1|1|1|0.75|0.6|5|201|617
chr1_2 exon 56727420 56728163 56727420 ID=Msa0079040-mRNA-1:exon:17845;Parent=Msa0079040-mRNA-1
chr1_2 exon 56728515 56729693 56728515 ID=Msa0079040-mRNA-1:exon:17844;Parent=Msa0079040-mRNA-1
chr1_2 exon 56729780 56729849 56729780 ID=Msa0079040-mRNA-1:exon:17843;Parent=Msa0079040-mRNA-1
chr1_2 exon 56732005 56732083 56732005 ID=Msa0079040-mRNA-1:exon:17842;Parent=Msa0079040-mRNA-1
chr1_2 exon 56732122 56732504 56732122 ID=Msa0079040-mRNA-1:exon:17841;Parent=Msa0079040-mRNA-1
chr1_2 five_prime_UTR 56732122 56732504 56732122 ID=Msa0079040-mRNA-1:five_prime_utr;Parent=Msa0079040-mRNA-1
chr1_2 five_prime_UTR 56732067 56732083 56732067 ID=Msa0079040-mRNA-1:five_prime_utr;Parent=Msa0079040-mRNA-1
chr1_2 CDS 56732005 56732066 56732005 ID=Msa0079040-mRNA-1:cds;Parent=Msa0079040-mRNA-1
chr1_2 CDS 56729780 56729849 56729780 ID=Msa0079040-mRNA-1:cds;Parent=Msa0079040-mRNA-1
chr1_2 CDS 56728515 56729693 56728515 ID=Msa0079040-mRNA-1:cds;Parent=Msa0079040-mRNA-1
chr1_2 CDS 56727621 56728163 56727621 ID=Msa0079040-mRNA-1:cds;Parent=Msa0079040-mRNA-1
chr1_2 three_prime_UTR 56727420 56727620 56727420 ID=Msa0079040-mRNA-1:three_prime_utr;Parent=Msa0079040-mRNA-1
Gene Sequence

>Msa0079040

ATGAAGTTTATGAAGTTGGGTTCGAAGCCTGATGCACTTCAATCTGATGGCAAATTTGTCAGGTACATATCATCTGAATTAGCTACAGATATTATCATAGCTATTGGTGATGTAAAGTTTGACCTTCACAAGTTCCCTCTACTGTCAAAGAGCAATCGCTTACAGAAGTTGGTGTCAAAGGCTAACGAAGATAATTCAGATGAAATTTACTTGGACGATTTCCCTGGTGGACCCAAGGCCTTTGAAATATGTGCAAAGTTCTGCTATGGAATGACTGTGACTCTGAATGCTTACAATGTTGTGGCTGCTCGATGTGCAGCGGAGTACCTTGAGATGACAGAGGATATTGATAAAGGAAATTTGATTTTTAAAATTGATGTGTTCCTTACATCAAGTATCTTTCGTAGCTGGAAGGATTCTATAATTGTTCTTCAAACTGCTAAATCTCTTCTCCCTTGGTCTGAGGATCTAAAGATAATTGGGAGATGCATAGATTCCATAGCATCAAAAACTTCTGTTGATCCGGCAAACATCACTTGGTCTTACACTTACAACCGAAAATTAACACAGGTCGATAAAGTCGTTGAGGACAAGATAACATTGAAAGAGAAAATTGAGTCTGTTCCGAAGGATTGGTGGGTTGAGGATATATGTGAGCTGGACATTGATCTTTATAAACGCGTAATGATAACGGTGAAATCTAAGGGAAGAATGGACGGTGTTGTGATTGGTGAGGCGCTGAAAACCTATGCTGTTAGATGGTTGCCTGATTCTGTTGATGCATTGGTTTCTGATGCTCATGCATGGAGGAACAAATCTCTGGTAGAAACAATTGTGTGTTTATTGCCGTGTGATAACGGTGTTGGTTGTTCCTGTAGTTTCTTGCTGAAACTATTTAAAGTGGCCATACTGGTTGAAGCCGATGAATCATCCAGAGAAGAATTGATGAACAGCATCAGCTTAAAATTGCATGAAGCTTCTGTCAAGGATTTACTGATTCCGGCTAGGTCTCCTGGGATTACTACTTATGATATTGATTTGGTGCAAGATCTTTTGAATCGATACATGACTAACGAAAAGAAAAAGCGTGATATTGAGGTTACAGATATGAAAGATAAAGTATTTGGTGAATCTATTTTAGGGAATAGGGCGATATTGAATCTCGGCAAGTTGGTTGATAGTTATCTTGGAGAAATTGCACATGATCCTAATCTTAGCCTCTCTAGTTTTGTTGATTTGTCAGAGTCAATTCCAGATTTTGCTAGACCAAATCATGACGGTTTGTACAGAGCCATCGATATCTACTTGAAGGAACACCCAAGTTTGACAAAATCTGAAAAGAAGAACATATGTGGACTGATGAATGTCAAGAAATTGACTGCTGATGCATCGGCGCATGCTGCACAGAACGAACGTCTTCCACTTCGAGTTGTAGTGCAGGTTCTGTATTTTGAACAAGTTAGAGCTGCAGCCAGTGCCCGAGCATTTGCTAACAGTCCACACAATCCTCCACATGCCCTAATAAATGGAGACGAGGAATGCGAGAAAACTCTGGGAGTGAGCTGCCATTCCCTCAACAGACAAACAAGTCGTTTGAAGATTAAAGACGATGAGTTTCGTAAGAGTGTGAGATTGGATAAAAAGAGTAGCAAAAATAGCAGAAGTGGCATGCAGTTGTTGCCGTCTCGGTCGAGGAGAATATTTGACAAGTTATGGAATGTTGGTAGTAAAGGGCATGGAGAGAATAGGAGTTCAGAGGCTTCAGGGAGTTCTAATAGTCCAACTTCTGTAGTCCTTGGAGATACAAAATCTTCTGGTTCATCCTTAAGACACAAAAGACATTCTATCTCTTAG
Protein sequence

>Msa0079040

MKFMKLGSKPDALQSDGKFVRYISSELATDIIIAIGDVKFDLHKFPLLSKSNRLQKLVSKANEDNSDEIYLDDFPGGPKAFEICAKFCYGMTVTLNAYNVVAARCAAEYLEMTEDIDKGNLIFKIDVFLTSSIFRSWKDSIIVLQTAKSLLPWSEDLKIIGRCIDSIASKTSVDPANITWSYTYNRKLTQVDKVVEDKITLKEKIESVPKDWWVEDICELDIDLYKRVMITVKSKGRMDGVVIGEALKTYAVRWLPDSVDALVSDAHAWRNKSLVETIVCLLPCDNGVGCSCSFLLKLFKVAILVEADESSREELMNSISLKLHEASVKDLLIPARSPGITTYDIDLVQDLLNRYMTNEKKKRDIEVTDMKDKVFGESILGNRAILNLGKLVDSYLGEIAHDPNLSLSSFVDLSESIPDFARPNHDGLYRAIDIYLKEHPSLTKSEKKNICGLMNVKKLTADASAHAAQNERLPLRVVVQVLYFEQVRAAASARAFANSPHNPPHALINGDEECEKTLGVSCHSLNRQTSRLKIKDDEFRKSVRLDKKSSKNSRSGMQLLPSRSRRIFDKLWNVGSKGHGENRSSEASGSSNSPTSVVLGDTKSSGSSLRHKRHSIS*