AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0005710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0005710 MtrunA17_Chr1g0150191 97.731 617 14 0 1 617 1 617 0.0 1237
Msa0005710 MtrunA17_Chr5g0401341 50.774 646 273 13 1 617 1 630 0.0 593
Msa0005710 MtrunA17_Chr1g0207461 48.523 643 287 15 1 617 1 625 0.0 551
Msa0005710 MtrunA17_Chr8g0367891 41.129 620 303 13 1 582 1 596 2.27e-136 410
Msa0005710 MtrunA17_Chr4g0076521 39.046 566 298 11 1 546 1 539 2.60e-134 405
Msa0005710 MtrunA17_Chr5g0412591 43.667 529 227 11 1 493 1 494 1.26e-132 401
Msa0005710 MtrunA17_Chr2g0279481 38.262 541 273 9 6 492 19 552 4.29e-123 379
Msa0005710 MtrunA17_Chr4g0050021 38.964 521 265 9 22 499 39 549 8.76e-123 378
Msa0005710 MtrunA17_Chr2g0290741 36.086 557 314 9 1 548 1 524 6.61e-119 365
Msa0005710 MtrunA17_Chr2g0285511 40.748 508 279 11 1 492 1 502 2.24e-116 359
Msa0005710 MtrunA17_Chr2g0303541 35.974 606 350 12 1 580 1 594 9.11e-116 359
Msa0005710 MtrunA17_Chr1g0181181 37.984 516 271 11 1 496 1 487 4.73e-104 326
Msa0005710 MtrunA17_Chr8g0382231 33.645 535 272 10 21 517 46 535 5.49e-104 328
Msa0005710 MtrunA17_Chr5g0401451 37.072 526 296 14 23 535 36 539 2.31e-102 323
Msa0005710 MtrunA17_Chr2g0320691 34.927 481 289 6 25 487 21 495 1.17e-97 311
Msa0005710 MtrunA17_Chr8g0374011 32.843 612 358 14 22 585 15 621 3.91e-97 311
Msa0005710 MtrunA17_Chr4g0010091 36.800 500 290 9 18 506 25 509 4.27e-97 310
Msa0005710 MtrunA17_Chr4g0053841 34.991 543 313 13 24 546 10 532 1.71e-95 305
Msa0005710 MtrunA17_Chr7g0245201 33.333 627 351 15 19 617 53 640 1.39e-92 298
Msa0005710 MtrunA17_Chr1g0195911 36.076 474 280 7 25 488 34 494 1.20e-89 290
Msa0005710 MtrunA17_Chr1g0199631 38.889 468 261 13 45 496 8 466 1.84e-87 283
Msa0005710 MtrunA17_Chr7g0268781 34.490 490 294 10 25 502 34 508 1.22e-82 272
Msa0005710 MtrunA17_Chr3g0108331 33.402 488 276 9 12 488 11 460 5.09e-80 263
Msa0005710 MtrunA17_Chr1g0191711 31.677 483 274 8 28 501 59 494 4.34e-79 260
Msa0005710 MtrunA17_Chr5g0432861 29.821 503 297 10 12 495 12 477 5.01e-73 245
Msa0005710 MtrunA17_Chr2g0332281 40.484 289 140 7 213 488 24 293 1.08e-56 197
Msa0005710 MtrunA17_Chr4g0003891 39.792 289 140 8 213 488 46 313 2.68e-53 189
Msa0005710 MtrunA17_Chr4g0037661 26.682 446 281 11 69 492 49 470 1.01e-38 150
Msa0005710 MtrunA17_Chr1g0163311 25.933 509 312 16 30 508 6 479 1.07e-38 149
Msa0005710 MtrunA17_Chr4g0023801 25.420 476 319 10 36 505 14 459 9.68e-38 147
Msa0005710 MtrunA17_Chr1g0206621 34.302 172 100 2 35 204 76 236 4.31e-28 112
Msa0005710 MtrunA17_Chr2g0280541 25.385 390 251 9 93 474 1 358 7.85e-23 101
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0005710 AT4G31820.1 57.966 590 187 13 1 581 1 538 0.0 651
Msa0005710 AT4G31820.2 56.797 537 171 13 54 581 1 485 0.0 572
Msa0005710 AT2G14820.2 52.906 499 223 4 1 493 1 493 1.20e-179 523
Msa0005710 AT2G14820.1 52.906 499 223 4 1 493 1 493 1.20e-179 523
Msa0005710 AT5G67440.3 46.942 605 272 11 1 597 1 564 4.27e-175 509
Msa0005710 AT5G67440.1 46.942 605 272 11 1 597 1 564 4.27e-175 509
Msa0005710 AT4G37590.1 46.387 595 248 16 1 573 1 546 1.00e-167 491
Msa0005710 AT2G23050.1 48.406 502 211 9 1 497 1 459 1.42e-157 461
Msa0005710 AT5G67440.2 45.213 564 260 11 42 597 5 527 2.33e-150 445
Msa0005710 AT1G67900.5 45.333 525 242 9 1 492 1 513 3.46e-142 427
Msa0005710 AT1G67900.1 45.333 525 242 9 1 492 1 513 3.46e-142 427
Msa0005710 AT1G67900.3 45.333 525 242 9 1 492 1 513 3.46e-142 427
Msa0005710 AT1G67900.6 45.333 525 242 9 1 492 1 513 3.46e-142 427
Msa0005710 AT1G67900.2 45.333 525 242 9 1 492 1 513 3.46e-142 427
Msa0005710 AT1G67900.4 45.333 525 242 9 1 492 1 513 3.46e-142 427
Msa0005710 AT3G26490.1 41.426 519 265 12 1 490 1 509 2.79e-124 379
Msa0005710 AT3G26490.3 41.426 519 265 12 1 490 1 509 2.79e-124 379
Msa0005710 AT3G26490.4 41.426 519 265 12 1 490 1 509 2.79e-124 379
Msa0005710 AT5G47800.3 38.067 507 286 6 1 499 1 487 6.07e-123 375
Msa0005710 AT5G47800.1 38.067 507 286 6 1 499 1 487 6.07e-123 375
Msa0005710 AT5G47800.4 38.067 507 286 6 1 499 1 487 6.07e-123 375
Msa0005710 AT5G47800.2 38.067 507 286 6 1 499 114 600 2.01e-121 375
Msa0005710 AT5G03250.1 39.038 520 283 11 1 496 1 510 4.54e-115 356
Msa0005710 AT2G23050.2 43.523 386 175 7 112 497 1 343 6.66e-105 322
Msa0005710 AT1G30440.1 35.922 515 304 8 1 492 1 512 8.93e-104 329
Msa0005710 AT3G26490.2 40.558 466 238 12 1 437 1 456 1.90e-102 320
Msa0005710 AT5G13600.1 37.500 536 294 15 1 512 1 519 4.29e-101 320
Msa0005710 AT5G48800.1 38.316 475 267 7 22 492 36 488 5.23e-97 310
Msa0005710 AT5G67385.1 36.786 473 271 9 24 488 22 474 1.49e-96 308
Msa0005710 AT3G44820.1 33.603 494 297 8 22 487 18 508 4.25e-91 295
Msa0005710 AT1G03010.3 35.863 527 288 14 12 511 18 521 1.84e-89 290
Msa0005710 AT5G66560.1 34.615 546 276 12 22 500 14 545 3.34e-89 290
Msa0005710 AT1G03010.2 35.938 512 280 13 25 511 7 495 7.53e-89 288
Msa0005710 AT1G03010.1 35.938 512 280 13 25 511 34 522 1.28e-88 288
Msa0005710 AT3G08660.1 33.450 571 313 12 19 567 26 551 4.61e-88 285
Msa0005710 AT5G10250.2 33.925 507 250 11 22 488 45 506 7.47e-88 286
Msa0005710 AT2G47860.1 37.344 482 262 11 25 488 35 494 2.52e-87 285
Msa0005710 AT3G08570.1 34.146 574 332 14 19 567 26 578 1.52e-86 282
Msa0005710 AT5G66560.3 34.528 530 266 11 38 500 1 516 5.18e-86 281
Msa0005710 AT5G67385.4 36.526 449 257 9 48 488 1 429 8.33e-86 278
Msa0005710 AT5G67385.5 36.526 449 257 9 48 488 1 429 8.33e-86 278
Msa0005710 AT5G67385.2 36.526 449 257 9 48 488 1 429 8.33e-86 278
Msa0005710 AT5G67385.3 36.526 449 257 9 48 488 1 429 8.33e-86 278
Msa0005710 AT5G10250.1 33.468 496 252 10 22 488 45 491 5.60e-85 278
Msa0005710 AT5G66560.4 37.363 455 248 10 69 500 66 506 3.10e-83 274
Msa0005710 AT3G50840.1 35.000 480 268 8 22 488 11 459 7.98e-83 271
Msa0005710 AT2G47860.3 35.644 505 262 12 25 488 35 517 2.78e-82 272
Msa0005710 AT2G30520.1 32.803 503 278 10 4 488 9 469 1.44e-80 266
Msa0005710 AT3G08660.3 33.080 526 286 11 64 567 10 491 2.18e-77 255
Msa0005710 AT3G49970.1 36.180 445 220 11 38 473 5 394 5.08e-76 251
Msa0005710 AT3G49970.2 36.180 445 220 11 38 473 5 394 5.08e-76 251
Msa0005710 AT3G50840.2 34.361 454 254 8 48 488 1 423 2.86e-73 245
Msa0005710 AT3G50840.3 34.361 454 254 8 48 488 1 423 2.86e-73 245
Msa0005710 AT5G03250.2 36.609 407 227 9 112 496 1 398 4.54e-73 243
Msa0005710 AT2G30520.2 33.043 460 250 11 47 488 2 421 2.19e-71 240
Msa0005710 AT2G30520.3 33.043 460 250 11 47 488 2 421 2.19e-71 240
Msa0005710 AT3G08660.2 34.434 424 239 10 19 437 26 415 1.66e-65 221
Msa0005710 AT2G47860.4 35.499 431 238 11 25 437 35 443 2.83e-65 221
Msa0005710 AT5G66560.2 31.875 480 249 10 22 437 14 479 2.62e-64 219
Msa0005710 AT5G64330.2 39.773 264 143 3 7 256 33 294 2.54e-62 218
Msa0005710 AT5G64330.2 37.959 245 132 5 271 499 373 613 3.76e-43 165
Msa0005710 AT5G64330.1 39.773 264 143 3 7 256 33 294 7.56e-62 219
Msa0005710 AT5G64330.1 37.959 245 132 5 271 499 373 613 3.63e-43 166
Msa0005710 AT2G47860.6 33.700 454 238 12 25 437 35 466 2.13e-60 209
Msa0005710 AT2G47860.5 35.443 395 218 10 112 488 1 376 2.74e-56 199
Msa0005710 AT2G47860.2 35.443 395 218 10 112 488 1 376 2.74e-56 199
Msa0005710 AT1G52770.1 37.288 295 147 6 213 488 28 303 1.95e-54 192
Msa0005710 AT5G64330.3 40.693 231 122 2 40 256 6 235 1.61e-53 193
Msa0005710 AT5G64330.3 37.959 245 132 5 271 499 314 554 1.38e-43 165
Msa0005710 AT5G48130.1 25.407 492 274 13 27 487 39 468 1.31e-52 191
Msa0005710 AT3G15570.1 34.783 299 153 7 213 488 29 308 3.46e-47 172
Msa0005710 AT3G19850.1 27.460 437 275 8 69 487 52 464 1.17e-46 173
Msa0005710 AT5G17580.2 27.484 473 305 10 23 488 16 457 1.21e-41 159
Msa0005710 AT5G17580.1 27.484 473 305 10 23 488 1 442 1.70e-41 158
Msa0005710 AT1G50280.2 27.804 428 277 8 69 482 18 427 1.14e-39 152
Msa0005710 AT1G50280.1 27.804 428 277 8 69 482 51 460 1.41e-39 152
Msa0005710 AT3G03510.1 29.762 420 225 15 77 488 4 361 1.31e-36 142
Msa0005710 AT3G22104.1 24.000 450 287 11 36 473 14 420 6.10e-32 130
Msa0005710 AT3G22104.4 23.117 385 243 9 99 473 4 345 3.12e-22 100
Msa0005710 AT3G22104.2 23.117 385 243 9 99 473 4 345 3.12e-22 100
Msa0005710 AT3G22104.3 23.117 385 243 9 99 473 4 345 3.12e-22 100

Find 147 sgRNAs with CRISPR-Local

Find 287 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGAATTACATAGAATTAAT+TGG 0.181392 1_1:+8397958 Msa0005710:exon
ATTCCTCGTCTCCATTTATT+AGG 0.216595 1_1:-8397622 None:intergenic
TCTGAGGATCTAAAGATAAT+TGG 0.223450 1_1:+8396214 Msa0005710:CDS
CAAAAGACATTCTATCTCTT+AGG 0.246837 1_1:+8397937 Msa0005710:CDS
CCTCCACATGCCCTAATAAA+TGG 0.253864 1_1:+8397611 Msa0005710:CDS
ATGTAATGTAATTTGAATTA+AGG 0.275791 1_1:+8398057 Msa0005710:three_prime_UTR
GATATGAAAGATAAAGTATT+TGG 0.280957 1_1:+8396859 Msa0005710:CDS
TGTCAAGGATTTACTGATTC+CGG 0.288586 1_1:+8396735 Msa0005710:CDS
CTGATTCCGGCTAGGTCTCC+TGG 0.293524 1_1:+8396748 Msa0005710:CDS
AATGATAACGGTGAAATCTA+AGG 0.298241 1_1:+8396438 Msa0005710:CDS
TGCACATATTTCAAAGGCCT+TGG 0.314739 1_1:-8395987 None:intergenic
GGAGAATATTTGACAAGTTA+TGG 0.336159 1_1:+8397798 Msa0005710:CDS
TACTACTTATGATATTGATT+TGG 0.339422 1_1:+8396774 Msa0005710:CDS
AAAATGAAGTTTATGAAGTT+GGG 0.342765 1_1:+8393535 Msa0005710:exon
CTGTACAAACCGTCATGATT+TGG 0.348424 1_1:-8397021 None:intergenic
ACTATTTAAAGTGGCCATAC+TGG 0.349780 1_1:+8396645 Msa0005710:CDS
CTTGGAGATACAAAATCTTC+TGG 0.359835 1_1:+8397899 Msa0005710:CDS
AATAGGAGTTCAGAGGCTTC+AGG 0.361947 1_1:+8397848 Msa0005710:CDS
TCCAAGATGAGTTAAGATTA+TGG 0.362037 1_1:-8393273 None:intergenic
TTTGTTTCTTTCTGTCTAGT+AGG 0.364013 1_1:+8398136 Msa0005710:three_prime_UTR
CCATCAGATTGAAGTGCATC+AGG 0.365409 1_1:-8393565 None:intergenic
TTCCTCGTCTCCATTTATTA+GGG 0.365614 1_1:-8397621 None:intergenic
CGTTGCAGAGAAAGTGTGTT+TGG 0.370622 1_1:+8393163 Msa0005710:five_prime_UTR
GCTGTTCATCAATTCTTCTC+TGG 0.372100 1_1:-8396686 None:intergenic
ACCATAATCTTAACTCATCT+TGG 0.388470 1_1:+8393272 Msa0005710:five_prime_UTR
ATGACAGAGGATATTGATAA+AGG 0.389164 1_1:+8396088 Msa0005710:CDS
GCCTGATTCTGTTGATGCAT+TGG 0.391893 1_1:+8396519 Msa0005710:CDS
ACCAATGCATCAACAGAATC+AGG 0.394719 1_1:-8396520 None:intergenic
AATCTGATGGCAAATTTGTC+AGG 0.409140 1_1:+8393578 Msa0005710:CDS
TTGTTTCTTTCTGTCTAGTA+GGG 0.411085 1_1:+8398137 Msa0005710:three_prime_UTR
GTTTCGTAAGAGTGTGAGAT+TGG 0.412767 1_1:+8397718 Msa0005710:CDS
CTGAGGATCTAAAGATAATT+GGG 0.417356 1_1:+8396215 Msa0005710:CDS
TGATTTGGTCTAGCAAAATC+TGG 0.421555 1_1:-8397006 None:intergenic
TTATGGAATGTTGGTAGTAA+AGG 0.422184 1_1:+8397815 Msa0005710:CDS
ACAGATATTATCATAGCTAT+TGG 0.425143 1_1:+8395753 Msa0005710:CDS
TATGGAATGTTGGTAGTAAA+GGG 0.426699 1_1:+8397816 Msa0005710:CDS
TAAGACCAAGTGATGTTTGC+CGG 0.434445 1_1:-8396277 None:intergenic
TTCTTCTCTGGATGATTCAT+CGG 0.435084 1_1:-8396674 None:intergenic
CATCAAGTATCTTTCGTAGC+TGG 0.435226 1_1:+8396143 Msa0005710:CDS
ATCTTAACTCATCTTGGACT+TGG 0.439734 1_1:+8393278 Msa0005710:five_prime_UTR
GATGAGTTAAGATTATGGTT+GGG 0.442387 1_1:-8393268 None:intergenic
CGAGGAATGCGAGAAAACTC+TGG 0.444156 1_1:+8397637 Msa0005710:CDS
GCACATATTTCAAAGGCCTT+GGG 0.445735 1_1:-8395986 None:intergenic
TAATTCAGATGAAATTTACT+TGG 0.448094 1_1:+8395946 Msa0005710:CDS
ACGACTTGTTTGTCTGTTGA+GGG 0.448196 1_1:-8397675 None:intergenic
AAAAGAGAGAAAACGTGTTA+AGG 0.451185 1_1:-8393410 None:intergenic
TTCATCGGCTTCAACCAGTA+TGG 0.453351 1_1:-8396659 None:intergenic
GCATGCAGTTGTTGCCGTCT+CGG 0.453505 1_1:+8397771 Msa0005710:CDS
AAGTTGGTTGATAGTTATCT+TGG 0.460673 1_1:+8396922 Msa0005710:CDS
CTTGCTGAAACTATTTAAAG+TGG 0.463625 1_1:+8396636 Msa0005710:CDS
TCCGAAGGATTGGTGGGTTG+AGG 0.463763 1_1:+8396378 Msa0005710:CDS
AAAAGACATTCTATCTCTTA+GGG 0.466446 1_1:+8397938 Msa0005710:CDS
TTTGACAAGTTATGGAATGT+TGG 0.466928 1_1:+8397806 Msa0005710:CDS
TCCTCAACCCACCAATCCTT+CGG 0.471360 1_1:-8396379 None:intergenic
TACGAAAGATACTTGATGTA+AGG 0.472293 1_1:-8396138 None:intergenic
AACGACTTGTTTGTCTGTTG+AGG 0.472812 1_1:-8397676 None:intergenic
ATAGGAGTTCAGAGGCTTCA+GGG 0.474284 1_1:+8397849 Msa0005710:CDS
GTTAGCAAATGCTCGGGCAC+TGG 0.475056 1_1:-8397576 None:intergenic
TTGAGTCTGTTCCGAAGGAT+TGG 0.477314 1_1:+8396368 Msa0005710:CDS
CTGCTAAATCTCTTCTCCCT+TGG 0.477644 1_1:+8396191 Msa0005710:CDS
GTAAAGGGCATGGAGAGAAT+AGG 0.477954 1_1:+8397831 Msa0005710:CDS
TGTTTATTGCCGTGTGATAA+CGG 0.480390 1_1:+8396592 Msa0005710:CDS
GAGCAATCGCTTACAGAAGT+TGG 0.481628 1_1:+8395904 Msa0005710:CDS
TCAAAGGCCTTGGGTCCACC+AGG 0.488895 1_1:-8395977 None:intergenic
AGTCCAACTTCTGTAGTCCT+TGG 0.489120 1_1:+8397881 Msa0005710:CDS
TTATAAACGCGTAATGATAA+CGG 0.489211 1_1:+8396426 Msa0005710:CDS
TGCATGGAGGAACAAATCTC+TGG 0.493898 1_1:+8396555 Msa0005710:CDS
TGAAAACCTATGCTGTTAGA+TGG 0.494881 1_1:+8396494 Msa0005710:CDS
ATGAGTTAAGATTATGGTTG+GGG 0.496428 1_1:-8393267 None:intergenic
AAAACGTGTTAAGGAAAGGA+AGG 0.498250 1_1:-8393401 None:intergenic
ATTTACTTGGACGATTTCCC+TGG 0.500638 1_1:+8395959 Msa0005710:CDS
AGATGAGTTAAGATTATGGT+TGG 0.502698 1_1:-8393269 None:intergenic
AGAATGGACGGTGTTGTGAT+TGG 0.502763 1_1:+8396463 Msa0005710:CDS
TGATTCCGGCTAGGTCTCCT+GGG 0.505713 1_1:+8396749 Msa0005710:CDS
GAAATTGACTGCTGATGCAT+CGG 0.507619 1_1:+8397475 Msa0005710:CDS
AGCCATCGATATCTACTTGA+AGG 0.509362 1_1:+8397044 Msa0005710:CDS
TCTCCAAGGACTACAGAAGT+TGG 0.509793 1_1:-8397884 None:intergenic
AAACTAGAGAGGCTAAGATT+AGG 0.511940 1_1:-8396961 None:intergenic
AGTCTGTTCCGAAGGATTGG+TGG 0.513817 1_1:+8396371 Msa0005710:CDS
ATCTTTAGATCCTCAGACCA+AGG 0.516375 1_1:-8396208 None:intergenic
TCCACTTCGAGTTGTAGTGC+AGG 0.516877 1_1:+8397523 Msa0005710:CDS
TGGTTTCTGATGCTCATGCA+TGG 0.521841 1_1:+8396539 Msa0005710:CDS
TTGTTTGTCTGTTGAGGGAA+TGG 0.522335 1_1:-8397670 None:intergenic
AGAGAAAACGTGTTAAGGAA+AGG 0.523073 1_1:-8393405 None:intergenic
GGTGAAATCTAAGGGAAGAA+TGG 0.523387 1_1:+8396447 Msa0005710:CDS
AATTAATGTGAAGTGTAAAG+TGG 0.523581 1_1:+8398002 Msa0005710:three_prime_UTR
CTTACAGAAGTTGGTGTCAA+AGG 0.524081 1_1:+8395913 Msa0005710:CDS
TTGATCCGGCAAACATCACT+TGG 0.527188 1_1:+8396272 Msa0005710:CDS
CGATTTCCCTGGTGGACCCA+AGG 0.529641 1_1:+8395970 Msa0005710:CDS
ATCTCTTCTCCCTTGGTCTG+AGG 0.535395 1_1:+8396198 Msa0005710:CDS
AGTTGTTGCCGTCTCGGTCG+AGG 0.536571 1_1:+8397777 Msa0005710:CDS
TTGCCGTGTGATAACGGTGT+TGG 0.537879 1_1:+8396598 Msa0005710:CDS
ACCTGCACTACAACTCGAAG+TGG 0.539338 1_1:-8397524 None:intergenic
CCTGATGCACTTCAATCTGA+TGG 0.542206 1_1:+8393565 Msa0005710:CDS
GAACTTTGCACATATTTCAA+AGG 0.543529 1_1:-8395993 None:intergenic
ATATGTGCAAAGTTCTGCTA+TGG 0.544022 1_1:+8396001 Msa0005710:CDS
ATGCAGTTAATAATGTTAGT+TGG 0.546007 1_1:-8393372 None:intergenic
TCTGAAAAGAAGAACATATG+TGG 0.548360 1_1:+8397437 Msa0005710:CDS
GATTGCTCTTTGACAGTAGA+GGG 0.548395 1_1:-8395889 None:intergenic
TACTTGGACGATTTCCCTGG+TGG 0.549505 1_1:+8395962 Msa0005710:CDS
GAAAATTGAGTCTGTTCCGA+AGG 0.556432 1_1:+8396363 Msa0005710:CDS
GATATTGAATCTCGGCAAGT+TGG 0.559092 1_1:+8396906 Msa0005710:CDS
ATGATAACGGTGAAATCTAA+GGG 0.563530 1_1:+8396439 Msa0005710:CDS
GTCTGTTCCGAAGGATTGGT+GGG 0.564671 1_1:+8396372 Msa0005710:CDS
AAGTGAAGAAGAGAGAGTAA+TGG 0.564864 1_1:-8393323 None:intergenic
TCTCCATTTATTAGGGCATG+TGG 0.566345 1_1:-8397614 None:intergenic
GGTTGAGGATATATGTGAGC+TGG 0.567054 1_1:+8396393 Msa0005710:CDS
CAATATCCTCTGTCATCTCA+AGG 0.567324 1_1:-8396081 None:intergenic
AGCAAAGTGTGAGATAGTTG+AGG 0.570159 1_1:-8393458 None:intergenic
ATTGCATGAAGCTTCTGTCA+AGG 0.573895 1_1:+8396720 Msa0005710:CDS
AGGATTTACTGATTCCGGCT+AGG 0.574506 1_1:+8396740 Msa0005710:CDS
TCTTACTTCACAACAAAACG+TGG 0.582158 1_1:+8393492 Msa0005710:five_prime_UTR
AAGTATCTTTCGTAGCTGGA+AGG 0.582873 1_1:+8396147 Msa0005710:CDS
GTGGACTGTTAGCAAATGCT+CGG 0.585051 1_1:-8397583 None:intergenic
AATATTCTCCTCGACCGAGA+CGG 0.585861 1_1:-8397785 None:intergenic
TAGTTTCAGCAAGAAACTAC+AGG 0.589035 1_1:-8396626 None:intergenic
CGATTGCTCTTTGACAGTAG+AGG 0.595990 1_1:-8395890 None:intergenic
GAGGAATGCGAGAAAACTCT+GGG 0.599575 1_1:+8397638 Msa0005710:CDS
TCTTTAGATCCTCAGACCAA+GGG 0.600214 1_1:-8396207 None:intergenic
GTAATCCCAGGAGACCTAGC+CGG 0.600747 1_1:-8396754 None:intergenic
CCATTTATTAGGGCATGTGG+AGG 0.601063 1_1:-8397611 None:intergenic
TCTGAATGCTTACAATGTTG+TGG 0.606892 1_1:+8396036 Msa0005710:CDS
TGGACTGTTAGCAAATGCTC+GGG 0.608053 1_1:-8397582 None:intergenic
AATGTTGGTAGTAAAGGGCA+TGG 0.608411 1_1:+8397821 Msa0005710:CDS
CTCAAAAGAGTGAATGAGAG+AGG 0.609313 1_1:-8393237 None:intergenic
AATAGGGCGATATTGAATCT+CGG 0.610706 1_1:+8396898 Msa0005710:CDS
TACCTTCAAGTAGATATCGA+TGG 0.617470 1_1:-8397046 None:intergenic
ATATCATAAGTAGTAATCCC+AGG 0.618972 1_1:-8396766 None:intergenic
AGGCAACCATCTAACAGCAT+AGG 0.619442 1_1:-8396500 None:intergenic
ACAAATCAACAAAACTAGAG+AGG 0.619836 1_1:-8396972 None:intergenic
TGGAGAGAATAGGAGTTCAG+AGG 0.626635 1_1:+8397841 Msa0005710:CDS
CAACCAACACCGTTATCACA+CGG 0.627146 1_1:-8396601 None:intergenic
TTTGCTAGACCAAATCATGA+CGG 0.635280 1_1:+8397012 Msa0005710:CDS
AAATCTAAGGGAAGAATGGA+CGG 0.638803 1_1:+8396451 Msa0005710:CDS
AATGTGAAGTGTAAAGTGGT+AGG 0.643484 1_1:+8398006 Msa0005710:three_prime_UTR
TGTGGCTGCTCGATGTGCAG+CGG 0.654801 1_1:+8396054 Msa0005710:CDS
ACAGGTCGATAAAGTCGTTG+AGG 0.658797 1_1:+8396321 Msa0005710:CDS
AGGGCATGTGGAGGATTGTG+TGG 0.662550 1_1:-8397602 None:intergenic
TGTAAAGTTTGACCTTCACA+AGG 0.678188 1_1:+8395779 Msa0005710:CDS
TGCCCTAATAAATGGAGACG+AGG 0.682274 1_1:+8397619 Msa0005710:CDS
CAAAGGCCTTGGGTCCACCA+GGG 0.683009 1_1:-8395976 None:intergenic
GGACGGTGTTGTGATTGGTG+AGG 0.691521 1_1:+8396468 Msa0005710:CDS
CTTACTTCACAACAAAACGT+GGG 0.693588 1_1:+8393493 Msa0005710:five_prime_UTR
TTACAACCGAAAATTAACAC+AGG 0.700722 1_1:+8396303 Msa0005710:CDS
GGAGTACCTTGAGATGACAG+AGG 0.738740 1_1:+8396075 Msa0005710:CDS
TTTCTGATGCTCATGCATGG+AGG 0.746693 1_1:+8396542 Msa0005710:CDS
TGAGAGAGGTCTGTACTCTG+TGG 0.751301 1_1:-8393223 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGATTATTTCATAAATATTA+TGG + chr1_1:8394755-8394774 Msa0005710:intron 10.0%
!! TATTAACATAAAAAAATGAT+TGG - chr1_1:8395110-8395129 None:intergenic 10.0%
!!! TTCATTTTTTTTTTCTTCTT+AGG - chr1_1:8393523-8393542 None:intergenic 15.0%
!!! AATATAAACTGAAATAGTTT+TGG + chr1_1:8393794-8393813 Msa0005710:intron 15.0%
!! TATGTTATTATTAACTGTAA+AGG + chr1_1:8394436-8394455 Msa0005710:intron 15.0%
!! ATGTTATTATTAACTGTAAA+GGG + chr1_1:8394437-8394456 Msa0005710:intron 15.0%
!! GTAAAACTAATGATATATAT+AGG - chr1_1:8394672-8394691 None:intergenic 15.0%
!! TATTTCATAAATATTATGGT+TGG + chr1_1:8394759-8394778 Msa0005710:intron 15.0%
!!! CTTGATTTTTTAACTTAATT+TGG - chr1_1:8394875-8394894 None:intergenic 15.0%
!! AATTAAGTTAAAAAATCAAG+AGG + chr1_1:8394875-8394894 Msa0005710:intron 15.0%
!! ATTAAGTTAAAAAATCAAGA+GGG + chr1_1:8394876-8394895 Msa0005710:intron 15.0%
!! TGATTAATATCAACAATTAT+TGG - chr1_1:8394972-8394991 None:intergenic 15.0%
!!! TGCAACTATTTTATTAAATA+AGG - chr1_1:8395165-8395184 None:intergenic 15.0%
!!! AGTTTTATTAAAAAATGCAA+TGG + chr1_1:8395477-8395496 Msa0005710:intron 15.0%
!! ATGTAATGTAATTTGAATTA+AGG + chr1_1:8398057-8398076 Msa0005710:three_prime_UTR 15.0%
!! AAAAATGAAGTTTATGAAGT+TGG + chr1_1:8393534-8393553 Msa0005710:exon 20.0%
!! AAAATGAAGTTTATGAAGTT+GGG + chr1_1:8393535-8393554 Msa0005710:exon 20.0%
!!! AATGTTTTAAAAACCAAACA+TGG + chr1_1:8393979-8393998 Msa0005710:intron 20.0%
!!! TGATTTTCTCAAATTATTAG+CGG + chr1_1:8394232-8394251 Msa0005710:intron 20.0%
!! ATATATTGAAACTATTTCAG+TGG - chr1_1:8394386-8394405 None:intergenic 20.0%
!! GTAGTGTACTATTAAATTAT+TGG + chr1_1:8394838-8394857 Msa0005710:intron 20.0%
!!! GATTTTTTAACTTAATTTGG+AGG - chr1_1:8394872-8394891 None:intergenic 20.0%
!! TTAATATCAACAATTATTGG+TGG - chr1_1:8394969-8394988 None:intergenic 20.0%
!!! ACTTTAAAAAGCTAGTATTT+TGG + chr1_1:8394998-8395017 Msa0005710:intron 20.0%
!!! TATTACATTTTTCTATCACT+AGG - chr1_1:8395458-8395477 None:intergenic 20.0%
!! TAATTCAGATGAAATTTACT+TGG + chr1_1:8395946-8395965 Msa0005710:CDS 20.0%
!!! TACTACTTATGATATTGATT+TGG + chr1_1:8396774-8396793 Msa0005710:CDS 20.0%
!!! GATATGAAAGATAAAGTATT+TGG + chr1_1:8396859-8396878 Msa0005710:CDS 20.0%
!!! TATTTGGTGAATCTATTTTA+GGG + chr1_1:8396875-8396894 Msa0005710:CDS 20.0%
!!! TTATTTTCTCGAATTATTTC+AGG - chr1_1:8397179-8397198 None:intergenic 20.0%
! ATGCAGTTAATAATGTTAGT+TGG - chr1_1:8393375-8393394 None:intergenic 25.0%
! CTAAAGAAACAAGAAAGTAA+AGG - chr1_1:8393664-8393683 None:intergenic 25.0%
! GCAACATAAAAAGATTCAAA+CGG - chr1_1:8393844-8393863 None:intergenic 25.0%
!! TTTGTTATGACAGTAAATCA+CGG + chr1_1:8393867-8393886 Msa0005710:intron 25.0%
!! AGTTTCTGGAAAATAAATCA+CGG - chr1_1:8393900-8393919 None:intergenic 25.0%
!!! TTTATTTTCCAGAAACTTTG+AGG + chr1_1:8393903-8393922 Msa0005710:intron 25.0%
! ACATTGCTTTGTATATGTTT+CGG - chr1_1:8393964-8393983 None:intergenic 25.0%
! TGAGAAAATCAAGTAATTCA+AGG - chr1_1:8394223-8394242 None:intergenic 25.0%
! TTGCATACAATGAGTTATAT+GGG - chr1_1:8394359-8394378 None:intergenic 25.0%
! TTTGCATACAATGAGTTATA+TGG - chr1_1:8394360-8394379 None:intergenic 25.0%
!!! ATAGTTTGCAAAGTCTTTTA+TGG + chr1_1:8394595-8394614 Msa0005710:intron 25.0%
! AAATAGTTGCAAGTATATGA+TGG + chr1_1:8395174-8395193 Msa0005710:intron 25.0%
! TAACTTATACAAGGAAAGAT+GGG - chr1_1:8395672-8395691 None:intergenic 25.0%
! TACAAAGTGTAACTTATACA+AGG - chr1_1:8395681-8395700 None:intergenic 25.0%
! AATTCATTTCCAATTTATGC+AGG + chr1_1:8395709-8395728 Msa0005710:intron 25.0%
! ACAGATATTATCATAGCTAT+TGG + chr1_1:8395753-8395772 Msa0005710:CDS 25.0%
! TTTAACAGGAAACTAATACA+AGG - chr1_1:8395823-8395842 None:intergenic 25.0%
! AGAAGAATTGCTTATTTAAC+AGG - chr1_1:8395837-8395856 None:intergenic 25.0%
! ACATGAGAAGTAAAAATCAA+TGG - chr1_1:8395860-8395879 None:intergenic 25.0%
! TTATAAACGCGTAATGATAA+CGG + chr1_1:8396426-8396445 Msa0005710:CDS 25.0%
!!! GTATTTGGTGAATCTATTTT+AGG + chr1_1:8396874-8396893 Msa0005710:CDS 25.0%
!!! TGAATCTATTTTAGGGAATA+GGG + chr1_1:8396882-8396901 Msa0005710:CDS 25.0%
!! TATTTTGTTAGTGTCATGTT+AGG - chr1_1:8397153-8397172 None:intergenic 25.0%
!!! TGTTCTAAAATGTGATATAC+TGG - chr1_1:8397308-8397327 None:intergenic 25.0%
! TATTAAGACAAAGAACAGAT+TGG - chr1_1:8397353-8397372 None:intergenic 25.0%
!! TTTCAGATTTTGTCAAACTT+GGG - chr1_1:8397424-8397443 None:intergenic 25.0%
!! TTTTCAGATTTTGTCAAACT+TGG - chr1_1:8397425-8397444 None:intergenic 25.0%
! AAAAGACATTCTATCTCTTA+GGG + chr1_1:8397938-8397957 Msa0005710:CDS 25.0%
! GGGAATTACATAGAATTAAT+TGG + chr1_1:8397958-8397977 Msa0005710:exon 25.0%
! AATTAATGTGAAGTGTAAAG+TGG + chr1_1:8398002-8398021 Msa0005710:three_prime_UTR 25.0%
ATGAGTTAAGATTATGGTTG+GGG - chr1_1:8393270-8393289 None:intergenic 30.0%
GATGAGTTAAGATTATGGTT+GGG - chr1_1:8393271-8393290 None:intergenic 30.0%
AGATGAGTTAAGATTATGGT+TGG - chr1_1:8393272-8393291 None:intergenic 30.0%
ACCATAATCTTAACTCATCT+TGG + chr1_1:8393272-8393291 Msa0005710:five_prime_UTR 30.0%
TCCAAGATGAGTTAAGATTA+TGG - chr1_1:8393276-8393295 None:intergenic 30.0%
!!! GACTTGGTTTTAAGCAAATA+AGG + chr1_1:8393294-8393313 Msa0005710:five_prime_UTR 30.0%
!!! TTGGTTTTAAGCAAATAAGG+CGG + chr1_1:8393297-8393316 Msa0005710:five_prime_UTR 30.0%
AAAAGAGAGAAAACGTGTTA+AGG - chr1_1:8393413-8393432 None:intergenic 30.0%
!!! TTTTTTTTTTCCCGTACTGT+TGG + chr1_1:8393704-8393723 Msa0005710:intron 30.0%
AATAGTAGTTAAATGTGTGC+TGG + chr1_1:8393743-8393762 Msa0005710:intron 30.0%
!!! ACTGAAATAGTTTTGGTGTT+TGG + chr1_1:8393801-8393820 Msa0005710:intron 30.0%
AAAAAGATTCAAACGGTTAC+TGG - chr1_1:8393837-8393856 None:intergenic 30.0%
CAATAAGTTTGTGAGAATCA+AGG - chr1_1:8393936-8393955 None:intergenic 30.0%
! GTGATTTTGCAAAAGCTATA+AGG + chr1_1:8394038-8394057 Msa0005710:intron 30.0%
! GTAATTATATGTGTCGATGT+TGG - chr1_1:8394201-8394220 None:intergenic 30.0%
! TGTAGCACATGATTTTTAAC+AGG - chr1_1:8394795-8394814 None:intergenic 30.0%
! ATTTGATCAAGTTTTGCGTT+TGG + chr1_1:8395321-8395340 Msa0005710:intron 30.0%
GAATCAAACTCAATACTTCA+GGG - chr1_1:8395436-8395455 None:intergenic 30.0%
! ACATGCATCTTAATACCTTT+TGG + chr1_1:8395533-8395552 Msa0005710:intron 30.0%
AACTTATACAAGGAAAGATG+GGG - chr1_1:8395671-8395690 None:intergenic 30.0%
GTAACTTATACAAGGAAAGA+TGG - chr1_1:8395673-8395692 None:intergenic 30.0%
TGATATGTACCTGCATAAAT+TGG - chr1_1:8395721-8395740 None:intergenic 30.0%
GAACTTTGCACATATTTCAA+AGG - chr1_1:8395996-8396015 None:intergenic 30.0%
ATGACAGAGGATATTGATAA+AGG + chr1_1:8396088-8396107 Msa0005710:CDS 30.0%
TACGAAAGATACTTGATGTA+AGG - chr1_1:8396141-8396160 None:intergenic 30.0%
TCTGAGGATCTAAAGATAAT+TGG + chr1_1:8396214-8396233 Msa0005710:CDS 30.0%
CTGAGGATCTAAAGATAATT+GGG + chr1_1:8396215-8396234 Msa0005710:CDS 30.0%
! AACAGAAGTTTTTGATGCTA+TGG - chr1_1:8396254-8396273 None:intergenic 30.0%
ATCAAAAACTTCTGTTGATC+CGG + chr1_1:8396258-8396277 Msa0005710:CDS 30.0%
TTACAACCGAAAATTAACAC+AGG + chr1_1:8396303-8396322 Msa0005710:CDS 30.0%
! TATCGACCTGTGTTAATTTT+CGG - chr1_1:8396312-8396331 None:intergenic 30.0%
!! AATGATAACGGTGAAATCTA+AGG + chr1_1:8396438-8396457 Msa0005710:CDS 30.0%
!! ATGATAACGGTGAAATCTAA+GGG + chr1_1:8396439-8396458 Msa0005710:CDS 30.0%
CTTGCTGAAACTATTTAAAG+TGG + chr1_1:8396636-8396655 Msa0005710:CDS 30.0%
ATATCATAAGTAGTAATCCC+AGG - chr1_1:8396769-8396788 None:intergenic 30.0%
! AAAGAAAAAGCGTGATATTG+AGG + chr1_1:8396831-8396850 Msa0005710:CDS 30.0%
!! GTGAATCTATTTTAGGGAAT+AGG + chr1_1:8396881-8396900 Msa0005710:CDS 30.0%
!! AAGTTGGTTGATAGTTATCT+TGG + chr1_1:8396922-8396941 Msa0005710:CDS 30.0%
ACAAATCAACAAAACTAGAG+AGG - chr1_1:8396975-8396994 None:intergenic 30.0%
!!! ATTTTTTGTCTGTGAAGCAT+TGG + chr1_1:8397113-8397132 Msa0005710:intron 30.0%
TCTGAAAAGAAGAACATATG+TGG + chr1_1:8397437-8397456 Msa0005710:CDS 30.0%
GGAGAATATTTGACAAGTTA+TGG + chr1_1:8397798-8397817 Msa0005710:CDS 30.0%
TTTGACAAGTTATGGAATGT+TGG + chr1_1:8397806-8397825 Msa0005710:CDS 30.0%
!! TTATGGAATGTTGGTAGTAA+AGG + chr1_1:8397815-8397834 Msa0005710:CDS 30.0%
!! TATGGAATGTTGGTAGTAAA+GGG + chr1_1:8397816-8397835 Msa0005710:CDS 30.0%
! AGAATGTCTTTTGTGTCTTA+AGG - chr1_1:8397930-8397949 None:intergenic 30.0%
CAAAAGACATTCTATCTCTT+AGG + chr1_1:8397937-8397956 Msa0005710:CDS 30.0%
! TTTGTTTCTTTCTGTCTAGT+AGG + chr1_1:8398136-8398155 Msa0005710:three_prime_UTR 30.0%
ATCTTAACTCATCTTGGACT+TGG + chr1_1:8393278-8393297 Msa0005710:five_prime_UTR 35.0%
AAGTGAAGAAGAGAGAGTAA+TGG - chr1_1:8393326-8393345 None:intergenic 35.0%
AAAACGTGTTAAGGAAAGGA+AGG - chr1_1:8393404-8393423 None:intergenic 35.0%
AGAGAAAACGTGTTAAGGAA+AGG - chr1_1:8393408-8393427 None:intergenic 35.0%
TCTTACTTCACAACAAAACG+TGG + chr1_1:8393492-8393511 Msa0005710:five_prime_UTR 35.0%
CTTACTTCACAACAAAACGT+GGG + chr1_1:8393493-8393512 Msa0005710:five_prime_UTR 35.0%
AATCTGATGGCAAATTTGTC+AGG + chr1_1:8393578-8393597 Msa0005710:CDS 35.0%
TAAGAAAACACCAACAGTAC+GGG - chr1_1:8393717-8393736 None:intergenic 35.0%
GTAAGAAAACACCAACAGTA+CGG - chr1_1:8393718-8393737 None:intergenic 35.0%
!! ATTTTCCAGAAACTTTGAGG+TGG + chr1_1:8393906-8393925 Msa0005710:intron 35.0%
GATTAGTAATGTCCCAATGT+CGG + chr1_1:8394169-8394188 Msa0005710:intron 35.0%
AAGATGCCAGCTATGAAATA+CGG - chr1_1:8394293-8394312 None:intergenic 35.0%
GTTATATGGGTTTCTCTTGA+AGG - chr1_1:8394346-8394365 None:intergenic 35.0%
AGAGAGAGAGGTTTAATTGA+AGG + chr1_1:8394476-8394495 Msa0005710:intron 35.0%
ACCAGAAACTCACAATTTGA+AGG - chr1_1:8394510-8394529 None:intergenic 35.0%
TCCAAACAGTGTGTATGTAT+AGG + chr1_1:8394541-8394560 Msa0005710:intron 35.0%
ACCTATACATACACACTGTT+TGG - chr1_1:8394545-8394564 None:intergenic 35.0%
AAACAGTGTGTATGTATAGG+TGG + chr1_1:8394544-8394563 Msa0005710:intron 35.0%
! TTTTAGCATGTGAGTTTCAC+TGG + chr1_1:8394693-8394712 Msa0005710:intron 35.0%
CTACACATGTATTCCACATT+TGG + chr1_1:8394810-8394829 Msa0005710:intron 35.0%
AGACATGCTTAACAAAGCAT+TGG - chr1_1:8394915-8394934 None:intergenic 35.0%
!!! TGCAGTTAGCCATTTTTGTA+GGG + chr1_1:8395028-8395047 Msa0005710:intron 35.0%
ATTTGCAGACCCTACAAAAA+TGG - chr1_1:8395040-8395059 None:intergenic 35.0%
TACACTAATAGTTTACCACC+AGG + chr1_1:8395368-8395387 Msa0005710:intron 35.0%
ACACTAATAGTTTACCACCA+GGG + chr1_1:8395369-8395388 Msa0005710:intron 35.0%
GGAATCAAACTCAATACTTC+AGG - chr1_1:8395437-8395456 None:intergenic 35.0%
! TGTTTTTCACCACATTCACA+TGG - chr1_1:8395516-8395535 None:intergenic 35.0%
TGTAAAGTTTGACCTTCACA+AGG + chr1_1:8395779-8395798 Msa0005710:CDS 35.0%
! AAAAAGTACACACCTTGTGA+AGG - chr1_1:8395794-8395813 None:intergenic 35.0%
ATATGTGCAAAGTTCTGCTA+TGG + chr1_1:8396001-8396020 Msa0005710:CDS 35.0%
TCTGAATGCTTACAATGTTG+TGG + chr1_1:8396036-8396055 Msa0005710:CDS 35.0%
AAATCTAAGGGAAGAATGGA+CGG + chr1_1:8396451-8396470 Msa0005710:CDS 35.0%
TGAAAACCTATGCTGTTAGA+TGG + chr1_1:8396494-8396513 Msa0005710:CDS 35.0%
TGTTTATTGCCGTGTGATAA+CGG + chr1_1:8396592-8396611 Msa0005710:CDS 35.0%
TAGTTTCAGCAAGAAACTAC+AGG - chr1_1:8396629-8396648 None:intergenic 35.0%
! ACTATTTAAAGTGGCCATAC+TGG + chr1_1:8396645-8396664 Msa0005710:CDS 35.0%
TTCTTCTCTGGATGATTCAT+CGG - chr1_1:8396677-8396696 None:intergenic 35.0%
!! TGTCAAGGATTTACTGATTC+CGG + chr1_1:8396735-8396754 Msa0005710:CDS 35.0%
AATAGGGCGATATTGAATCT+CGG + chr1_1:8396898-8396917 Msa0005710:CDS 35.0%
AAACTAGAGAGGCTAAGATT+AGG - chr1_1:8396964-8396983 None:intergenic 35.0%
!! TGATTTGGTCTAGCAAAATC+TGG - chr1_1:8397009-8397028 None:intergenic 35.0%
TTTGCTAGACCAAATCATGA+CGG + chr1_1:8397012-8397031 Msa0005710:CDS 35.0%
TACCTTCAAGTAGATATCGA+TGG - chr1_1:8397049-8397068 None:intergenic 35.0%
! AAGGTAAGATTTTGCTACCT+TGG + chr1_1:8397063-8397082 Msa0005710:intron 35.0%
! CATTTGCGTGAATCATTTTC+AGG + chr1_1:8397394-8397413 Msa0005710:intron 35.0%
TTCCTCGTCTCCATTTATTA+GGG - chr1_1:8397624-8397643 None:intergenic 35.0%
ATTCCTCGTCTCCATTTATT+AGG - chr1_1:8397625-8397644 None:intergenic 35.0%
AGTAGCAAAAATAGCAGAAG+TGG + chr1_1:8397749-8397768 Msa0005710:CDS 35.0%
! CAGAAGATTTTGTATCTCCA+AGG - chr1_1:8397901-8397920 None:intergenic 35.0%
CTTGGAGATACAAAATCTTC+TGG + chr1_1:8397899-8397918 Msa0005710:CDS 35.0%
AATGTGAAGTGTAAAGTGGT+AGG + chr1_1:8398006-8398025 Msa0005710:three_prime_UTR 35.0%
CTCAAAAGAGTGAATGAGAG+AGG - chr1_1:8393240-8393259 None:intergenic 40.0%
AGCAAAGTGTGAGATAGTTG+AGG - chr1_1:8393461-8393480 None:intergenic 40.0%
GTAAACCACCTCAAAGTTTC+TGG - chr1_1:8393914-8393933 None:intergenic 40.0%
ATTGGGACATTACTAATCCG+AGG - chr1_1:8394167-8394186 None:intergenic 40.0%
CCAGCTATGAAATACGGATA+CGG - chr1_1:8394287-8394306 None:intergenic 40.0%
GAGAGAGGTTTAATTGAAGG+TGG + chr1_1:8394479-8394498 Msa0005710:intron 40.0%
GAAACTCACAATTTGAAGGC+TGG - chr1_1:8394506-8394525 None:intergenic 40.0%
GCCTTCAAATTGTGAGTTTC+TGG + chr1_1:8394506-8394525 Msa0005710:intron 40.0%
GTGTGTATGTATAGGTGGTA+TGG + chr1_1:8394549-8394568 Msa0005710:intron 40.0%
TACACTACACTGACCAAATG+TGG - chr1_1:8394826-8394845 None:intergenic 40.0%
CTACAAAAATGGCTAACTGC+AGG - chr1_1:8395029-8395048 None:intergenic 40.0%
!!! CTGCAGTTAGCCATTTTTGT+AGG + chr1_1:8395027-8395046 Msa0005710:intron 40.0%
GTGATGTATCCATGTGAATG+TGG + chr1_1:8395504-8395523 Msa0005710:intron 40.0%
GCAACTGAAATTCAGCCAAA+AGG - chr1_1:8395551-8395570 None:intergenic 40.0%
ATTTGCATGCCTACATTGTG+TGG + chr1_1:8395604-8395623 Msa0005710:intron 40.0%
TTTGCATGCCTACATTGTGT+GGG + chr1_1:8395605-8395624 Msa0005710:intron 40.0%
ACAATTACCCCACACAATGT+AGG - chr1_1:8395616-8395635 None:intergenic 40.0%
GATTGCTCTTTGACAGTAGA+GGG - chr1_1:8395892-8395911 None:intergenic 40.0%
!! CTTACAGAAGTTGGTGTCAA+AGG + chr1_1:8395913-8395932 Msa0005710:CDS 40.0%
ATTTACTTGGACGATTTCCC+TGG + chr1_1:8395959-8395978 Msa0005710:CDS 40.0%
GCACATATTTCAAAGGCCTT+GGG - chr1_1:8395989-8396008 None:intergenic 40.0%
TGCACATATTTCAAAGGCCT+TGG - chr1_1:8395990-8396009 None:intergenic 40.0%
CAATATCCTCTGTCATCTCA+AGG - chr1_1:8396084-8396103 None:intergenic 40.0%
CATCAAGTATCTTTCGTAGC+TGG + chr1_1:8396143-8396162 Msa0005710:CDS 40.0%
AAGTATCTTTCGTAGCTGGA+AGG + chr1_1:8396147-8396166 Msa0005710:CDS 40.0%
TCTTTAGATCCTCAGACCAA+GGG - chr1_1:8396210-8396229 None:intergenic 40.0%
ATCTTTAGATCCTCAGACCA+AGG - chr1_1:8396211-8396230 None:intergenic 40.0%
TAAGACCAAGTGATGTTTGC+CGG - chr1_1:8396280-8396299 None:intergenic 40.0%
GAAAATTGAGTCTGTTCCGA+AGG + chr1_1:8396363-8396382 Msa0005710:CDS 40.0%
GGTGAAATCTAAGGGAAGAA+TGG + chr1_1:8396447-8396466 Msa0005710:CDS 40.0%
ACCAATGCATCAACAGAATC+AGG - chr1_1:8396523-8396542 None:intergenic 40.0%
GCTGTTCATCAATTCTTCTC+TGG - chr1_1:8396689-8396708 None:intergenic 40.0%
ATTGCATGAAGCTTCTGTCA+AGG + chr1_1:8396720-8396739 Msa0005710:CDS 40.0%
GATATTGAATCTCGGCAAGT+TGG + chr1_1:8396906-8396925 Msa0005710:CDS 40.0%
CTGTACAAACCGTCATGATT+TGG - chr1_1:8397024-8397043 None:intergenic 40.0%
AGCCATCGATATCTACTTGA+AGG + chr1_1:8397044-8397063 Msa0005710:CDS 40.0%
! GTAAGATTTTGCTACCTTGG+AGG + chr1_1:8397066-8397085 Msa0005710:intron 40.0%
AAGAAGTGAAAACACCTCCA+AGG - chr1_1:8397083-8397102 None:intergenic 40.0%
! ATGTCTTCCACAGAAAGTGT+CGG + chr1_1:8397226-8397245 Msa0005710:intron 40.0%
! TAGTTGTGCAAGCATTGTTC+AGG - chr1_1:8397285-8397304 None:intergenic 40.0%
GAAATTGACTGCTGATGCAT+CGG + chr1_1:8397475-8397494 Msa0005710:CDS 40.0%
TCTCCATTTATTAGGGCATG+TGG - chr1_1:8397617-8397636 None:intergenic 40.0%
TTGTTTGTCTGTTGAGGGAA+TGG - chr1_1:8397673-8397692 None:intergenic 40.0%
! ACGACTTGTTTGTCTGTTGA+GGG - chr1_1:8397678-8397697 None:intergenic 40.0%
! AACGACTTGTTTGTCTGTTG+AGG - chr1_1:8397679-8397698 None:intergenic 40.0%
! GTTTCGTAAGAGTGTGAGAT+TGG + chr1_1:8397718-8397737 Msa0005710:CDS 40.0%
!! AATGTTGGTAGTAAAGGGCA+TGG + chr1_1:8397821-8397840 Msa0005710:CDS 40.0%
! CGTTGCAGAGAAAGTGTGTT+TGG + chr1_1:8393163-8393182 Msa0005710:five_prime_UTR 45.0%
CCATCAGATTGAAGTGCATC+AGG - chr1_1:8393568-8393587 None:intergenic 45.0%
CCTGATGCACTTCAATCTGA+TGG + chr1_1:8393565-8393584 Msa0005710:CDS 45.0%
GATAGGATGTCTGCTAGGTT+CGG - chr1_1:8394130-8394149 None:intergenic 45.0%
!! AGGAGTGTTTGTGCTTCGAT+AGG - chr1_1:8394147-8394166 None:intergenic 45.0%
ATCGAAGCACAAACACTCCT+CGG + chr1_1:8394147-8394166 Msa0005710:intron 45.0%
TGAAATACGGATACGGACAC+CGG - chr1_1:8394280-8394299 None:intergenic 45.0%
CCGTATCCGTATTTCATAGC+TGG + chr1_1:8394284-8394303 Msa0005710:intron 45.0%
!! TGAGTTTCTGGTTGAGCTTG+TGG + chr1_1:8394518-8394537 Msa0005710:intron 45.0%
CAACTACTTTCACTACACCC+TGG - chr1_1:8395389-8395408 None:intergenic 45.0%
CAGGGTGTAGTGAAAGTAGT+TGG + chr1_1:8395387-8395406 Msa0005710:intron 45.0%
TTGCATGCCTACATTGTGTG+GGG + chr1_1:8395606-8395625 Msa0005710:intron 45.0%
CGATTGCTCTTTGACAGTAG+AGG - chr1_1:8395893-8395912 None:intergenic 45.0%
GAGCAATCGCTTACAGAAGT+TGG + chr1_1:8395904-8395923 Msa0005710:CDS 45.0%
CTGCTAAATCTCTTCTCCCT+TGG + chr1_1:8396191-8396210 Msa0005710:CDS 45.0%
TTGATCCGGCAAACATCACT+TGG + chr1_1:8396272-8396291 Msa0005710:CDS 45.0%
ACAGGTCGATAAAGTCGTTG+AGG + chr1_1:8396321-8396340 Msa0005710:CDS 45.0%
TTGAGTCTGTTCCGAAGGAT+TGG + chr1_1:8396368-8396387 Msa0005710:CDS 45.0%
GGTTGAGGATATATGTGAGC+TGG + chr1_1:8396393-8396412 Msa0005710:CDS 45.0%
!! AGAATGGACGGTGTTGTGAT+TGG + chr1_1:8396463-8396482 Msa0005710:CDS 45.0%
AGGCAACCATCTAACAGCAT+AGG - chr1_1:8396503-8396522 None:intergenic 45.0%
GCCTGATTCTGTTGATGCAT+TGG + chr1_1:8396519-8396538 Msa0005710:CDS 45.0%
TGGTTTCTGATGCTCATGCA+TGG + chr1_1:8396539-8396558 Msa0005710:CDS 45.0%
TTTCTGATGCTCATGCATGG+AGG + chr1_1:8396542-8396561 Msa0005710:CDS 45.0%
TGCATGGAGGAACAAATCTC+TGG + chr1_1:8396555-8396574 Msa0005710:CDS 45.0%
CAACCAACACCGTTATCACA+CGG - chr1_1:8396604-8396623 None:intergenic 45.0%
!! TTCATCGGCTTCAACCAGTA+TGG - chr1_1:8396662-8396681 None:intergenic 45.0%
!! AGGATTTACTGATTCCGGCT+AGG + chr1_1:8396740-8396759 Msa0005710:CDS 45.0%
!! TATAGCACCGACACTTTCTG+TGG - chr1_1:8397236-8397255 None:intergenic 45.0%
TGGACTGTTAGCAAATGCTC+GGG - chr1_1:8397585-8397604 None:intergenic 45.0%
! GTGGACTGTTAGCAAATGCT+CGG - chr1_1:8397586-8397605 None:intergenic 45.0%
CCATTTATTAGGGCATGTGG+AGG - chr1_1:8397614-8397633 None:intergenic 45.0%
CCTCCACATGCCCTAATAAA+TGG + chr1_1:8397611-8397630 Msa0005710:CDS 45.0%
TGCCCTAATAAATGGAGACG+AGG + chr1_1:8397619-8397638 Msa0005710:CDS 45.0%
AATATTCTCCTCGACCGAGA+CGG - chr1_1:8397788-8397807 None:intergenic 45.0%
! GTAAAGGGCATGGAGAGAAT+AGG + chr1_1:8397831-8397850 Msa0005710:CDS 45.0%
TGGAGAGAATAGGAGTTCAG+AGG + chr1_1:8397841-8397860 Msa0005710:CDS 45.0%
AATAGGAGTTCAGAGGCTTC+AGG + chr1_1:8397848-8397867 Msa0005710:CDS 45.0%
ATAGGAGTTCAGAGGCTTCA+GGG + chr1_1:8397849-8397868 Msa0005710:CDS 45.0%
AGTCCAACTTCTGTAGTCCT+TGG + chr1_1:8397881-8397900 Msa0005710:CDS 45.0%
TCTCCAAGGACTACAGAAGT+TGG - chr1_1:8397887-8397906 None:intergenic 45.0%
TGAGAGAGGTCTGTACTCTG+TGG - chr1_1:8393226-8393245 None:intergenic 50.0%
ACCAAACATGGTACCGCGTT+AGG + chr1_1:8393991-8394010 Msa0005710:intron 50.0%
ACCTAACGCGGTACCATGTT+TGG - chr1_1:8393995-8394014 None:intergenic 50.0%
GCTTCGATAGGATGTCTGCT+AGG - chr1_1:8394135-8394154 None:intergenic 50.0%
TGTTGGACACGACCGACATT+GGG - chr1_1:8394184-8394203 None:intergenic 50.0%
ATGTTGGACACGACCGACAT+TGG - chr1_1:8394185-8394204 None:intergenic 50.0%
AGAGTCTGAGAGAGAGAGAG+AGG + chr1_1:8394464-8394483 Msa0005710:intron 50.0%
CTACTTTCACTACACCCTGG+TGG - chr1_1:8395386-8395405 None:intergenic 50.0%
! GTGAAAGTAGTTGGTGCGCA+TGG + chr1_1:8395396-8395415 Msa0005710:intron 50.0%
! TAGTTGGTGCGCATGGTATG+TGG + chr1_1:8395403-8395422 Msa0005710:intron 50.0%
! AGTTGGTGCGCATGGTATGT+GGG + chr1_1:8395404-8395423 Msa0005710:intron 50.0%
TACTTGGACGATTTCCCTGG+TGG + chr1_1:8395962-8395981 Msa0005710:CDS 50.0%
! GGAGTACCTTGAGATGACAG+AGG + chr1_1:8396075-8396094 Msa0005710:CDS 50.0%
ATCTCTTCTCCCTTGGTCTG+AGG + chr1_1:8396198-8396217 Msa0005710:CDS 50.0%
AGTCTGTTCCGAAGGATTGG+TGG + chr1_1:8396371-8396390 Msa0005710:CDS 50.0%
! GTCTGTTCCGAAGGATTGGT+GGG + chr1_1:8396372-8396391 Msa0005710:CDS 50.0%
TCCTCAACCCACCAATCCTT+CGG - chr1_1:8396382-8396401 None:intergenic 50.0%
!! TTGCCGTGTGATAACGGTGT+TGG + chr1_1:8396598-8396617 Msa0005710:CDS 50.0%
!! TCCACTTCGAGTTGTAGTGC+AGG + chr1_1:8397523-8397542 Msa0005710:CDS 50.0%
ACCTGCACTACAACTCGAAG+TGG - chr1_1:8397527-8397546 None:intergenic 50.0%
! ATGGTACCGCGTTAGGTTGC+CGG + chr1_1:8393998-8394017 Msa0005710:intron 55.0%
GTGATTCCGGCAACCTAACG+CGG - chr1_1:8394007-8394026 None:intergenic 55.0%
! GCGTTAGGTTGCCGGAATCA+CGG + chr1_1:8394006-8394025 Msa0005710:intron 55.0%
! TTAGGTTGCCGGAATCACGG+TGG + chr1_1:8394009-8394028 Msa0005710:intron 55.0%
ACTGCTAGCCACCGTGATTC+CGG - chr1_1:8394020-8394039 None:intergenic 55.0%
GTTGGTGCGCATGGTATGTG+GGG + chr1_1:8395405-8395424 Msa0005710:intron 55.0%
!! TCCGAAGGATTGGTGGGTTG+AGG + chr1_1:8396378-8396397 Msa0005710:CDS 55.0%
!! GGACGGTGTTGTGATTGGTG+AGG + chr1_1:8396468-8396487 Msa0005710:CDS 55.0%
TGATTCCGGCTAGGTCTCCT+GGG + chr1_1:8396749-8396768 Msa0005710:CDS 55.0%
GTAATCCCAGGAGACCTAGC+CGG - chr1_1:8396757-8396776 None:intergenic 55.0%
GTTAGCAAATGCTCGGGCAC+TGG - chr1_1:8397579-8397598 None:intergenic 55.0%
AGGGCATGTGGAGGATTGTG+TGG - chr1_1:8397605-8397624 None:intergenic 55.0%
GCATGCAGTTGTTGCCGTCT+CGG + chr1_1:8397771-8397790 Msa0005710:CDS 55.0%
CAGCGTCTCAGTGTCGTCTC+CGG + chr1_1:8394258-8394277 Msa0005710:intron 60.0%
! CGATTTCCCTGGTGGACCCA+AGG + chr1_1:8395970-8395989 Msa0005710:CDS 60.0%
CAAAGGCCTTGGGTCCACCA+GGG - chr1_1:8395979-8395998 None:intergenic 60.0%
TCAAAGGCCTTGGGTCCACC+AGG - chr1_1:8395980-8395999 None:intergenic 60.0%
TGTGGCTGCTCGATGTGCAG+CGG + chr1_1:8396054-8396073 Msa0005710:CDS 60.0%
CTGATTCCGGCTAGGTCTCC+TGG + chr1_1:8396748-8396767 Msa0005710:CDS 60.0%
AGTTGTTGCCGTCTCGGTCG+AGG + chr1_1:8397777-8397796 Msa0005710:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 8393145 8398159 8393145 ID=Msa0005710;Name=Msa0005710
chr1_1 mRNA 8393145 8398159 8393145 ID=Msa0005710-mRNA-1;Parent=Msa0005710;Name=Msa0005710-mRNA-1;_AED=0.01;_eAED=0.01;_QI=393|1|1|1|1|1|4|201|617
chr1_1 exon 8393145 8393599 8393145 ID=Msa0005710-mRNA-1:exon:2978;Parent=Msa0005710-mRNA-1
chr1_1 exon 8395731 8395800 8395731 ID=Msa0005710-mRNA-1:exon:2979;Parent=Msa0005710-mRNA-1
chr1_1 exon 8395887 8397065 8395887 ID=Msa0005710-mRNA-1:exon:2980;Parent=Msa0005710-mRNA-1
chr1_1 exon 8397416 8398159 8397416 ID=Msa0005710-mRNA-1:exon:2981;Parent=Msa0005710-mRNA-1
chr1_1 five_prime_UTR 8393145 8393537 8393145 ID=Msa0005710-mRNA-1:five_prime_utr;Parent=Msa0005710-mRNA-1
chr1_1 CDS 8393538 8393599 8393538 ID=Msa0005710-mRNA-1:cds;Parent=Msa0005710-mRNA-1
chr1_1 CDS 8395731 8395800 8395731 ID=Msa0005710-mRNA-1:cds;Parent=Msa0005710-mRNA-1
chr1_1 CDS 8395887 8397065 8395887 ID=Msa0005710-mRNA-1:cds;Parent=Msa0005710-mRNA-1
chr1_1 CDS 8397416 8397958 8397416 ID=Msa0005710-mRNA-1:cds;Parent=Msa0005710-mRNA-1
chr1_1 three_prime_UTR 8397959 8398159 8397959 ID=Msa0005710-mRNA-1:three_prime_utr;Parent=Msa0005710-mRNA-1
Gene Sequence

>Msa0005710

ATGAAGTTTATGAAGTTGGGTTCGAAGCCTGATGCACTTCAATCTGATGGCAAATTTGTCAGGTACATATCATCTGAATTAGCTACAGATATTATCATAGCTATTGGTGATGTAAAGTTTGACCTTCACAAGTTCCCTCTACTGTCAAAGAGCAATCGCTTACAGAAGTTGGTGTCAAAGGCTAACGAAGATAATTCAGATGAAATTTACTTGGACGATTTCCCTGGTGGACCCAAGGCCTTTGAAATATGTGCAAAGTTCTGCTATGGAATGACTGTGACTCTGAATGCTTACAATGTTGTGGCTGCTCGATGTGCAGCGGAGTACCTTGAGATGACAGAGGATATTGATAAAGGAAATTTGATTTTTAAAATTGATGTGTTCCTTACATCAAGTATCTTTCGTAGCTGGAAGGATTCTATAATTGTTCTTCAAACTGCTAAATCTCTTCTCCCTTGGTCTGAGGATCTAAAGATAATTGGGAGATGCATAGATTCCATAGCATCAAAAACTTCTGTTGATCCGGCAAACATCACTTGGTCTTACACTTACAACCGAAAATTAACACAGGTCGATAAAGTCGTTGAGGACAAGATAACATTGAAAGAGAAAATTGAGTCTGTTCCGAAGGATTGGTGGGTTGAGGATATATGTGAGCTGGACATTGATCTTTATAAACGCGTAATGATAACGGTGAAATCTAAGGGAAGAATGGACGGTGTTGTGATTGGTGAGGCGCTGAAAACCTATGCTGTTAGATGGTTGCCTGATTCTGTTGATGCATTGGTTTCTGATGCTCATGCATGGAGGAACAAATCTCTGGTAGAAACAATTGTGTGTTTATTGCCGTGTGATAACGGTGTTGGTTGTTCCTGTAGTTTCTTGCTGAAACTATTTAAAGTGGCCATACTGGTTGAAGCCGATGAATCATCCAGAGAAGAATTGATGAACAGCATCAGCTTAAAATTGCATGAAGCTTCTGTCAAGGATTTACTGATTCCGGCTAGGTCTCCTGGGATTACTACTTATGATATTGATTTGGTGCAAGATCTTTTGAATCGATACATGACTAACGAAAAGAAAAAGCGTGATATTGAGGTTACAGATATGAAAGATAAAGTATTTGGTGAATCTATTTTAGGGAATAGGGCGATATTGAATCTCGGCAAGTTGGTTGATAGTTATCTTGGAGAAATTGCACATGATCCTAATCTTAGCCTCTCTAGTTTTGTTGATTTGTCAGAGTCAATTCCAGATTTTGCTAGACCAAATCATGACGGTTTGTACAGAGCCATCGATATCTACTTGAAGGAACACCCAAGTTTGACAAAATCTGAAAAGAAGAACATATGTGGACTGATGAATGTCAAGAAATTGACTGCTGATGCATCGGCGCATGCTGCACAGAACGAACGTCTTCCACTTCGAGTTGTAGTGCAGGTTCTGTATTTTGAACAAGTTAGAGCTGCAGCCAGTGCCCGAGCATTTGCTAACAGTCCACACAATCCTCCACATGCCCTAATAAATGGAGACGAGGAATGCGAGAAAACTCTGGGAGTGAGCTGCCATTCCCTCAACAGACAAACAAGTCGTTTGAAGATTAAAGACGATGAGTTTCGTAAGAGTGTGAGATTGGATAAAAAGAGTAGCAAAAATAGCAGAAGTGGCATGCAGTTGTTGCCGTCTCGGTCGAGGAGAATATTTGACAAGTTATGGAATGTTGGTAGTAAAGGGCATGGAGAGAATAGGAGTTCAGAGGCTTCAGGGAGTTCTAATAGTCCAACTTCTGTAGTCCTTGGAGATACAAAATCTTCTGGTTCATCCTTAAGACACAAAAGACATTCTATCTCTTAG
Protein sequence

>Msa0005710

MKFMKLGSKPDALQSDGKFVRYISSELATDIIIAIGDVKFDLHKFPLLSKSNRLQKLVSKANEDNSDEIYLDDFPGGPKAFEICAKFCYGMTVTLNAYNVVAARCAAEYLEMTEDIDKGNLIFKIDVFLTSSIFRSWKDSIIVLQTAKSLLPWSEDLKIIGRCIDSIASKTSVDPANITWSYTYNRKLTQVDKVVEDKITLKEKIESVPKDWWVEDICELDIDLYKRVMITVKSKGRMDGVVIGEALKTYAVRWLPDSVDALVSDAHAWRNKSLVETIVCLLPCDNGVGCSCSFLLKLFKVAILVEADESSREELMNSISLKLHEASVKDLLIPARSPGITTYDIDLVQDLLNRYMTNEKKKRDIEVTDMKDKVFGESILGNRAILNLGKLVDSYLGEIAHDPNLSLSSFVDLSESIPDFARPNHDGLYRAIDIYLKEHPSLTKSEKKNICGLMNVKKLTADASAHAAQNERLPLRVVVQVLYFEQVRAAASARAFANSPHNPPHALINGDEECEKTLGVSCHSLNRQTSRLKIKDDEFRKSVRLDKKSSKNSRSGMQLLPSRSRRIFDKLWNVGSKGHGENRSSEASGSSNSPTSVVLGDTKSSGSSLRHKRHSIS*