AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0181730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0181730 MtrunA17_Chr1g0202691 96.277 188 7 0 19 206 3 190 1.61e-134 375
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0181730 AT4G25670.2 54.054 185 81 3 23 206 1 182 1.53e-50 162
Msa0181730 AT4G25670.1 54.054 185 81 3 23 206 1 182 1.53e-50 162
Msa0181730 AT4G25690.2 51.795 195 73 5 23 206 1 185 2.40e-49 159
Msa0181730 AT4G25690.1 51.795 195 73 5 23 206 1 185 2.40e-49 159
Msa0181730 AT5G52550.1 50.820 183 67 6 38 206 181 354 1.01e-43 150
Msa0181730 AT5G52550.1 73.333 60 16 0 38 97 78 137 1.53e-17 80.1

Find 57 sgRNAs with CRISPR-Local

Find 290 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCCTCTCTCATATGTTAT+CGG 0.243854 1_4:-69973637 Msa0181730:five_prime_UTR
AATGGCATAGAGAGAGAAAT+TGG 0.272654 1_4:+69973431 None:intergenic
GATAACATGGCATGTAATAA+AGG 0.286451 1_4:-69971963 Msa0181730:CDS
AGATAGACCACATGCCGTTT+TGG 0.307332 1_4:+69971583 None:intergenic
AACATGTATATTATTGTAAT+AGG 0.311130 1_4:+69972687 None:intergenic
TAATAATCTTGATTTCTTCA+TGG 0.361966 1_4:-69971661 Msa0181730:CDS
TATGTCGTTATTAAGTTCAA+TGG 0.374544 1_4:-69971985 Msa0181730:intron
TCTCTCATATGTTATCGGTT+TGG 0.388640 1_4:-69973632 Msa0181730:five_prime_UTR
GCTTGCTTTCCTAATCTAGA+TGG 0.392945 1_4:-69971627 Msa0181730:CDS
AGACTCAGATGATTCATATA+AGG 0.397214 1_4:-69971712 Msa0181730:CDS
TGCTGCCATCATATCGGAAC+TGG 0.404529 1_4:-69971832 Msa0181730:CDS
AATAATCTTGATTTCTTCAT+GGG 0.406348 1_4:-69971660 Msa0181730:CDS
AAACGAGAAGTGATTGTTAG+TGG 0.410114 1_4:+69973498 None:intergenic
AGAGGAAGAGAAAATGTAGT+TGG 0.425893 1_4:+69973529 None:intergenic
TCTCTCTATGCCATTCCCTC+AGG 0.434714 1_4:-69973423 Msa0181730:intron
TACTTCTGCTGCCATCATAT+CGG 0.438376 1_4:-69971838 Msa0181730:CDS
CTCAAATCACCACTCTCATC+AGG 0.457188 1_4:-69973462 Msa0181730:five_prime_UTR
ATAACATGGCATGTAATAAA+GGG 0.468465 1_4:-69971962 Msa0181730:CDS
CATGTGCTACCATCTAGATT+AGG 0.471484 1_4:+69971618 None:intergenic
CACGAACCACTTTACGAAGC+AGG 0.483308 1_4:-69971534 Msa0181730:CDS
TAAGGTTGAGTGCAAGGCCT+GGG 0.484400 1_4:-69971694 Msa0181730:CDS
AGATCAACAGAGAATCTTGT+AGG 0.486420 1_4:+69971504 None:intergenic
TTATAGCAGCACAAGCAGTT+AGG 0.486837 1_4:-69971482 Msa0181730:CDS
GAGGAAGAGAAAATGTAGTT+GGG 0.489801 1_4:+69973530 None:intergenic
ATCTTGATTTCTTCATGGGT+GGG 0.489941 1_4:-69971656 Msa0181730:CDS
GAAGCAGGTGAAAGATGAGC+TGG 0.492593 1_4:-69971913 Msa0181730:CDS
GAGAAGTGATTGTTAGTGGT+TGG 0.499250 1_4:+69973502 None:intergenic
TGGTCCGTCACTTCCCAAAA+CGG 0.505701 1_4:-69971597 Msa0181730:CDS
TTGTTAGTGGTTGGATCTAG+AGG 0.515473 1_4:+69973511 None:intergenic
AGGATAGGATTCTCTTCTAA+AGG 0.516327 1_4:-69971431 None:intergenic
AATCTTGATTTCTTCATGGG+TGG 0.519569 1_4:-69971657 Msa0181730:CDS
CAATAATATACATGTTAAGT+AGG 0.519901 1_4:-69972680 Msa0181730:intron
TCCCACCCTGCTTCGTAAAG+TGG 0.522362 1_4:+69971528 None:intergenic
CAGAGAATGCAGATGAGGAT+AGG 0.524026 1_4:-69971446 Msa0181730:CDS
ATAAGGTTGAGTGCAAGGCC+TGG 0.527429 1_4:-69971695 Msa0181730:CDS
GGCATGTGGTCTATCTCGTC+CGG 0.532370 1_4:-69971576 Msa0181730:CDS
AAGAGAAAATGTAGTTGGGT+AGG 0.532815 1_4:+69973534 None:intergenic
GAGAAAGACAAAGAAGAAGC+AGG 0.540706 1_4:-69971928 Msa0181730:CDS
TTCATATAAGGTTGAGTGCA+AGG 0.541798 1_4:-69971700 Msa0181730:CDS
ATGAAACAAACCTGAGGGAA+TGG 0.542301 1_4:+69973413 None:intergenic
ATTATTATTATAATCATCCC+AGG 0.551179 1_4:+69971677 None:intergenic
GAGTAGAACATGCAGAGCGA+CGG 0.587047 1_4:+69971740 None:intergenic
CATCTGCATTCTCTGCAATG+TGG 0.593120 1_4:+69971454 None:intergenic
ATTAAGTTCAATGGATAACA+TGG 0.608143 1_4:-69971976 Msa0181730:intron
CATTGCAGAGAATGCAGATG+AGG 0.616435 1_4:-69971451 Msa0181730:CDS
TCACTTCCCAAAACGGCATG+TGG 0.617380 1_4:-69971590 Msa0181730:CDS
ACAGCAAAGACTTGATGAAG+AGG 0.619614 1_4:-69971781 Msa0181730:CDS
ACGAACCACTTTACGAAGCA+GGG 0.620702 1_4:-69971533 Msa0181730:CDS
TGAAGAGGGTGCTGCAATTG+CGG 0.628142 1_4:-69971766 Msa0181730:CDS
ACCACTTTACGAAGCAGGGT+GGG 0.630797 1_4:-69971529 Msa0181730:CDS
CTAATCTAGATGGTAGCACA+TGG 0.631528 1_4:-69971617 Msa0181730:CDS
CAGCAAAGACTTGATGAAGA+GGG 0.636235 1_4:-69971780 Msa0181730:CDS
GTAATAGGATCTGCTTGCAG+TGG 0.648746 1_4:+69972702 None:intergenic
AACCACTTTACGAAGCAGGG+TGG 0.676621 1_4:-69971530 Msa0181730:CDS
AAACCGATAACATATGAGAG+AGG 0.679221 1_4:+69973634 None:intergenic
GATGAGAGTGGTGATTTGAG+AGG 0.706629 1_4:+69973465 None:intergenic
GAGACAAGACCTGATGAGAG+TGG 0.721166 1_4:+69973453 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTTAAAAACAAAACAAAAA+AGG + chr1_4:69971721-69971740 None:intergenic 15.0%
!!! AATTTTGATTTGATTGTATT+TGG - chr1_4:69971750-69971769 Msa0181730:CDS 15.0%
!!! TGTGTTTTTTTTTATGAATT+AGG - chr1_4:69971900-69971919 Msa0181730:CDS 15.0%
!! AACATGTATATTATTGTAAT+AGG + chr1_4:69972399-69972418 None:intergenic 15.0%
!! TAAATATACAATTCATTTAC+AGG + chr1_4:69972640-69972659 None:intergenic 15.0%
!! TATTATTAGCATAATAGTTT+AGG - chr1_4:69972993-69973012 Msa0181730:intron 15.0%
!! ATTATTAGCATAATAGTTTA+GGG - chr1_4:69972994-69973013 Msa0181730:intron 15.0%
!! AGTTAAAAACAAAACAAAAA+AGG + chr1_4:69971721-69971740 None:intergenic 15.0%
!!! AATTTTGATTTGATTGTATT+TGG - chr1_4:69971750-69971769 Msa0181730:CDS 15.0%
!!! TGTGTTTTTTTTTATGAATT+AGG - chr1_4:69971900-69971919 Msa0181730:CDS 15.0%
!! AACATGTATATTATTGTAAT+AGG + chr1_4:69972399-69972418 None:intergenic 15.0%
!! TAAATATACAATTCATTTAC+AGG + chr1_4:69972640-69972659 None:intergenic 15.0%
!! TATTATTAGCATAATAGTTT+AGG - chr1_4:69972993-69973012 Msa0181730:intron 15.0%
!! ATTATTAGCATAATAGTTTA+GGG - chr1_4:69972994-69973013 Msa0181730:intron 15.0%
!!! ATCTTGTGATTTTTGTAATT+TGG - chr1_4:69971844-69971863 Msa0181730:CDS 20.0%
!!! TCTTGTGATTTTTGTAATTT+GGG - chr1_4:69971845-69971864 Msa0181730:CDS 20.0%
!!! GTTTTTTTTTATGAATTAGG+TGG - chr1_4:69971903-69971922 Msa0181730:CDS 20.0%
!!! TCGATGTTTTTGTTATTTTT+AGG - chr1_4:69971940-69971959 Msa0181730:CDS 20.0%
!!! CGATGTTTTTGTTATTTTTA+GGG - chr1_4:69971941-69971960 Msa0181730:CDS 20.0%
!!! GTTCAATTTTGATATTTGTT+AGG - chr1_4:69972159-69972178 Msa0181730:intron 20.0%
!! CAATAATATACATGTTAAGT+AGG - chr1_4:69972403-69972422 Msa0181730:intron 20.0%
!! TACTCTTAAATATCATTTGA+TGG - chr1_4:69972535-69972554 Msa0181730:intron 20.0%
!!! TTTAAAGTAATTTTCGTTTG+TGG - chr1_4:69972563-69972582 Msa0181730:intron 20.0%
!!! ACATTAAAATTCACTTTTAG+AGG + chr1_4:69972611-69972630 None:intergenic 20.0%
!! TTATTAGCATAATAGTTTAG+GGG - chr1_4:69972995-69973014 Msa0181730:intron 20.0%
!! ATTATTATTATAATCATCCC+AGG + chr1_4:69973409-69973428 None:intergenic 20.0%
!! TAATAATCTTGATTTCTTCA+TGG - chr1_4:69973422-69973441 Msa0181730:intron 20.0%
!! AATAATCTTGATTTCTTCAT+GGG - chr1_4:69973423-69973442 Msa0181730:intron 20.0%
!!! ATCTTGTGATTTTTGTAATT+TGG - chr1_4:69971844-69971863 Msa0181730:CDS 20.0%
!!! TCTTGTGATTTTTGTAATTT+GGG - chr1_4:69971845-69971864 Msa0181730:CDS 20.0%
!!! GTTTTTTTTTATGAATTAGG+TGG - chr1_4:69971903-69971922 Msa0181730:CDS 20.0%
!!! TCGATGTTTTTGTTATTTTT+AGG - chr1_4:69971940-69971959 Msa0181730:CDS 20.0%
!!! CGATGTTTTTGTTATTTTTA+GGG - chr1_4:69971941-69971960 Msa0181730:CDS 20.0%
!!! GTTCAATTTTGATATTTGTT+AGG - chr1_4:69972159-69972178 Msa0181730:intron 20.0%
!! CAATAATATACATGTTAAGT+AGG - chr1_4:69972403-69972422 Msa0181730:intron 20.0%
!! TACTCTTAAATATCATTTGA+TGG - chr1_4:69972535-69972554 Msa0181730:intron 20.0%
!!! TTTAAAGTAATTTTCGTTTG+TGG - chr1_4:69972563-69972582 Msa0181730:intron 20.0%
!!! ACATTAAAATTCACTTTTAG+AGG + chr1_4:69972611-69972630 None:intergenic 20.0%
!! TTATTAGCATAATAGTTTAG+GGG - chr1_4:69972995-69973014 Msa0181730:intron 20.0%
!! ATTATTATTATAATCATCCC+AGG + chr1_4:69973409-69973428 None:intergenic 20.0%
!! TAATAATCTTGATTTCTTCA+TGG - chr1_4:69973422-69973441 Msa0181730:intron 20.0%
!! AATAATCTTGATTTCTTCAT+GGG - chr1_4:69973423-69973442 Msa0181730:intron 20.0%
! ACAAAAAAGGCAAAATTTGA+AGG + chr1_4:69971708-69971727 None:intergenic 25.0%
!! CTTGTGATTTTTGTAATTTG+GGG - chr1_4:69971846-69971865 Msa0181730:CDS 25.0%
! TCAAGCTTACTGAATATAAT+TGG - chr1_4:69972040-69972059 Msa0181730:intron 25.0%
! ACTTAACATGAAATGTTAAC+AGG + chr1_4:69972228-69972247 None:intergenic 25.0%
! AATAATGCACAGATATATAG+AGG - chr1_4:69972436-69972455 Msa0181730:intron 25.0%
! TACAATAACAGACAATACTT+GGG + chr1_4:69972499-69972518 None:intergenic 25.0%
! TTACAATAACAGACAATACT+TGG + chr1_4:69972500-69972519 None:intergenic 25.0%
! GAAAATGTATACAACTCTAT+AGG - chr1_4:69973030-69973049 Msa0181730:intron 25.0%
! TATGTCGTTATTAAGTTCAA+TGG - chr1_4:69973098-69973117 Msa0181730:intron 25.0%
! ATTAAGTTCAATGGATAACA+TGG - chr1_4:69973107-69973126 Msa0181730:intron 25.0%
! ATAACATGGCATGTAATAAA+GGG - chr1_4:69973121-69973140 Msa0181730:intron 25.0%
! ACAAAAAAGGCAAAATTTGA+AGG + chr1_4:69971708-69971727 None:intergenic 25.0%
!! CTTGTGATTTTTGTAATTTG+GGG - chr1_4:69971846-69971865 Msa0181730:CDS 25.0%
! TCAAGCTTACTGAATATAAT+TGG - chr1_4:69972040-69972059 Msa0181730:intron 25.0%
! ACTTAACATGAAATGTTAAC+AGG + chr1_4:69972228-69972247 None:intergenic 25.0%
! AATAATGCACAGATATATAG+AGG - chr1_4:69972436-69972455 Msa0181730:intron 25.0%
! TACAATAACAGACAATACTT+GGG + chr1_4:69972499-69972518 None:intergenic 25.0%
! TTACAATAACAGACAATACT+TGG + chr1_4:69972500-69972519 None:intergenic 25.0%
! GAAAATGTATACAACTCTAT+AGG - chr1_4:69973030-69973049 Msa0181730:intron 25.0%
! TATGTCGTTATTAAGTTCAA+TGG - chr1_4:69973098-69973117 Msa0181730:intron 25.0%
! ATTAAGTTCAATGGATAACA+TGG - chr1_4:69973107-69973126 Msa0181730:intron 25.0%
! ATAACATGGCATGTAATAAA+GGG - chr1_4:69973121-69973140 Msa0181730:intron 25.0%
TTTCCTCTCTCATATGTTAT+CGG - chr1_4:69971446-69971465 Msa0181730:CDS 30.0%
GATTTGATTGTATTTGGATC+TGG - chr1_4:69971756-69971775 Msa0181730:CDS 30.0%
AATTCGAGTGATTCAATTTG+AGG - chr1_4:69971790-69971809 Msa0181730:CDS 30.0%
TTTGTTGCAAATAGATTTGC+AGG - chr1_4:69971969-69971988 Msa0181730:CDS 30.0%
AGATTTGCAGGAATGAAAAT+TGG - chr1_4:69971981-69972000 Msa0181730:intron 30.0%
GATTTGCAGGAATGAAAATT+GGG - chr1_4:69971982-69972001 Msa0181730:intron 30.0%
ATTGCATAGTTCGTAAAATG+AGG - chr1_4:69972891-69972910 Msa0181730:intron 30.0%
GATAACATGGCATGTAATAA+AGG - chr1_4:69973120-69973139 Msa0181730:intron 30.0%
AAGCTGAAAAGAAAAAGAGA+CGG - chr1_4:69973205-69973224 Msa0181730:intron 30.0%
AGACTCAGATGATTCATATA+AGG - chr1_4:69973371-69973390 Msa0181730:intron 30.0%
TTTCCTCTCTCATATGTTAT+CGG - chr1_4:69971446-69971465 Msa0181730:CDS 30.0%
GATTTGATTGTATTTGGATC+TGG - chr1_4:69971756-69971775 Msa0181730:CDS 30.0%
AATTCGAGTGATTCAATTTG+AGG - chr1_4:69971790-69971809 Msa0181730:CDS 30.0%
TTTGTTGCAAATAGATTTGC+AGG - chr1_4:69971969-69971988 Msa0181730:CDS 30.0%
AGATTTGCAGGAATGAAAAT+TGG - chr1_4:69971981-69972000 Msa0181730:intron 30.0%
GATTTGCAGGAATGAAAATT+GGG - chr1_4:69971982-69972001 Msa0181730:intron 30.0%
ATTGCATAGTTCGTAAAATG+AGG - chr1_4:69972891-69972910 Msa0181730:intron 30.0%
GATAACATGGCATGTAATAA+AGG - chr1_4:69973120-69973139 Msa0181730:intron 30.0%
AAGCTGAAAAGAAAAAGAGA+CGG - chr1_4:69973205-69973224 Msa0181730:intron 30.0%
AGACTCAGATGATTCATATA+AGG - chr1_4:69973371-69973390 Msa0181730:intron 30.0%
AAACCGATAACATATGAGAG+AGG + chr1_4:69971452-69971471 None:intergenic 35.0%
! TCTCTCATATGTTATCGGTT+TGG - chr1_4:69971451-69971470 Msa0181730:CDS 35.0%
AAGAGAAAATGTAGTTGGGT+AGG + chr1_4:69971552-69971571 None:intergenic 35.0%
GAGGAAGAGAAAATGTAGTT+GGG + chr1_4:69971556-69971575 None:intergenic 35.0%
AGAGGAAGAGAAAATGTAGT+TGG + chr1_4:69971557-69971576 None:intergenic 35.0%
AAACGAGAAGTGATTGTTAG+TGG + chr1_4:69971588-69971607 None:intergenic 35.0%
AATGGCATAGAGAGAGAAAT+TGG + chr1_4:69971655-69971674 None:intergenic 35.0%
GATGAATGAAACAAACCTGA+GGG + chr1_4:69971678-69971697 None:intergenic 35.0%
TGATGAATGAAACAAACCTG+AGG + chr1_4:69971679-69971698 None:intergenic 35.0%
TGAATTAGGTGGATCTGATT+AGG - chr1_4:69971914-69971933 Msa0181730:CDS 35.0%
GAATTAGGTGGATCTGATTA+GGG - chr1_4:69971915-69971934 Msa0181730:CDS 35.0%
TTGTTAGGTGAGTTAGATGT+TGG - chr1_4:69972174-69972193 Msa0181730:intron 35.0%
! GTACTAGTCTATTGTACACT+TGG - chr1_4:69972246-69972265 Msa0181730:intron 35.0%
TAGTCTATTGTACACTTGGT+TGG - chr1_4:69972250-69972269 Msa0181730:intron 35.0%
! TAATTTTCGTTTGTGGTGAC+TGG - chr1_4:69972570-69972589 Msa0181730:intron 35.0%
TTAAGGGGAAAAACTCAAAG+TGG + chr1_4:69972672-69972691 None:intergenic 35.0%
TTGTGGTAAGTTAGGAAGAA+TGG - chr1_4:69972799-69972818 Msa0181730:intron 35.0%
TGTGGTAAGTTAGGAAGAAT+GGG - chr1_4:69972800-69972819 Msa0181730:intron 35.0%
! TAGGAGTTTGCTTGAAGAAT+AGG - chr1_4:69973049-69973068 Msa0181730:intron 35.0%
! AGGAGTTTGCTTGAAGAATA+GGG - chr1_4:69973050-69973069 Msa0181730:intron 35.0%
TGAAAAGAAAAAGAGACGGT+TGG - chr1_4:69973209-69973228 Msa0181730:intron 35.0%
! TTTTTCCAGTTCCGATATGA+TGG + chr1_4:69973259-69973278 None:intergenic 35.0%
TTCATATAAGGTTGAGTGCA+AGG - chr1_4:69973383-69973402 Msa0181730:intron 35.0%
AATCTTGATTTCTTCATGGG+TGG - chr1_4:69973426-69973445 Msa0181730:exon 35.0%
ATCTTGATTTCTTCATGGGT+GGG - chr1_4:69973427-69973446 Msa0181730:exon 35.0%
AGATCAACAGAGAATCTTGT+AGG + chr1_4:69973582-69973601 None:intergenic 35.0%
AAACCGATAACATATGAGAG+AGG + chr1_4:69971452-69971471 None:intergenic 35.0%
! TCTCTCATATGTTATCGGTT+TGG - chr1_4:69971451-69971470 Msa0181730:CDS 35.0%
AAGAGAAAATGTAGTTGGGT+AGG + chr1_4:69971552-69971571 None:intergenic 35.0%
GAGGAAGAGAAAATGTAGTT+GGG + chr1_4:69971556-69971575 None:intergenic 35.0%
AGAGGAAGAGAAAATGTAGT+TGG + chr1_4:69971557-69971576 None:intergenic 35.0%
AAACGAGAAGTGATTGTTAG+TGG + chr1_4:69971588-69971607 None:intergenic 35.0%
AATGGCATAGAGAGAGAAAT+TGG + chr1_4:69971655-69971674 None:intergenic 35.0%
GATGAATGAAACAAACCTGA+GGG + chr1_4:69971678-69971697 None:intergenic 35.0%
TGATGAATGAAACAAACCTG+AGG + chr1_4:69971679-69971698 None:intergenic 35.0%
TGAATTAGGTGGATCTGATT+AGG - chr1_4:69971914-69971933 Msa0181730:CDS 35.0%
GAATTAGGTGGATCTGATTA+GGG - chr1_4:69971915-69971934 Msa0181730:CDS 35.0%
TTGTTAGGTGAGTTAGATGT+TGG - chr1_4:69972174-69972193 Msa0181730:intron 35.0%
! GTACTAGTCTATTGTACACT+TGG - chr1_4:69972246-69972265 Msa0181730:intron 35.0%
TAGTCTATTGTACACTTGGT+TGG - chr1_4:69972250-69972269 Msa0181730:intron 35.0%
! TAATTTTCGTTTGTGGTGAC+TGG - chr1_4:69972570-69972589 Msa0181730:intron 35.0%
TTAAGGGGAAAAACTCAAAG+TGG + chr1_4:69972672-69972691 None:intergenic 35.0%
TTGTGGTAAGTTAGGAAGAA+TGG - chr1_4:69972799-69972818 Msa0181730:intron 35.0%
TGTGGTAAGTTAGGAAGAAT+GGG - chr1_4:69972800-69972819 Msa0181730:intron 35.0%
! TAGGAGTTTGCTTGAAGAAT+AGG - chr1_4:69973049-69973068 Msa0181730:intron 35.0%
! AGGAGTTTGCTTGAAGAATA+GGG - chr1_4:69973050-69973069 Msa0181730:intron 35.0%
TGAAAAGAAAAAGAGACGGT+TGG - chr1_4:69973209-69973228 Msa0181730:intron 35.0%
! TTTTTCCAGTTCCGATATGA+TGG + chr1_4:69973259-69973278 None:intergenic 35.0%
TTCATATAAGGTTGAGTGCA+AGG - chr1_4:69973383-69973402 Msa0181730:intron 35.0%
AATCTTGATTTCTTCATGGG+TGG - chr1_4:69973426-69973445 Msa0181730:exon 35.0%
ATCTTGATTTCTTCATGGGT+GGG - chr1_4:69973427-69973446 Msa0181730:exon 35.0%
AGATCAACAGAGAATCTTGT+AGG + chr1_4:69973582-69973601 None:intergenic 35.0%
TTGTTAGTGGTTGGATCTAG+AGG + chr1_4:69971575-69971594 None:intergenic 40.0%
GAGAAGTGATTGTTAGTGGT+TGG + chr1_4:69971584-69971603 None:intergenic 40.0%
ATGAAACAAACCTGAGGGAA+TGG + chr1_4:69971673-69971692 None:intergenic 40.0%
! TACACTTGGTTGGTTAAACC+AGG - chr1_4:69972260-69972279 Msa0181730:intron 40.0%
ATGAACATGTCAGATCAACC+TGG + chr1_4:69972281-69972300 None:intergenic 40.0%
! TTTTCCCCTTAAACTGCAGT+TGG - chr1_4:69972680-69972699 Msa0181730:intron 40.0%
TTTCCCCTTAAACTGCAGTT+GGG - chr1_4:69972681-69972700 Msa0181730:CDS 40.0%
TAACAGTATTGCAGACCTGT+TGG - chr1_4:69972749-69972768 Msa0181730:intron 40.0%
! TGCAAGTGTTGTGGTAAGTT+AGG - chr1_4:69972791-69972810 Msa0181730:intron 40.0%
GTGGTAAGTTAGGAAGAATG+GGG - chr1_4:69972801-69972820 Msa0181730:intron 40.0%
GAGAAAGACAAAGAAGAAGC+AGG - chr1_4:69973155-69973174 Msa0181730:intron 40.0%
TACTTCTGCTGCCATCATAT+CGG - chr1_4:69973245-69973264 Msa0181730:intron 40.0%
ACAGCAAAGACTTGATGAAG+AGG - chr1_4:69973302-69973321 Msa0181730:intron 40.0%
CAGCAAAGACTTGATGAAGA+GGG - chr1_4:69973303-69973322 Msa0181730:intron 40.0%
GCTTGCTTTCCTAATCTAGA+TGG - chr1_4:69973456-69973475 Msa0181730:five_prime_UTR 40.0%
CATGTGCTACCATCTAGATT+AGG + chr1_4:69973468-69973487 None:intergenic 40.0%
CTAATCTAGATGGTAGCACA+TGG - chr1_4:69973466-69973485 Msa0181730:five_prime_UTR 40.0%
! CACATTGTTTTCGAAAGGTC+CGG + chr1_4:69973529-69973548 None:intergenic 40.0%
! TCGTGCACATTGTTTTCGAA+AGG + chr1_4:69973534-69973553 None:intergenic 40.0%
TTATAGCAGCACAAGCAGTT+AGG - chr1_4:69973601-69973620 Msa0181730:five_prime_UTR 40.0%
TTGTTAGTGGTTGGATCTAG+AGG + chr1_4:69971575-69971594 None:intergenic 40.0%
GAGAAGTGATTGTTAGTGGT+TGG + chr1_4:69971584-69971603 None:intergenic 40.0%
ATGAAACAAACCTGAGGGAA+TGG + chr1_4:69971673-69971692 None:intergenic 40.0%
! TACACTTGGTTGGTTAAACC+AGG - chr1_4:69972260-69972279 Msa0181730:intron 40.0%
ATGAACATGTCAGATCAACC+TGG + chr1_4:69972281-69972300 None:intergenic 40.0%
! TTTTCCCCTTAAACTGCAGT+TGG - chr1_4:69972680-69972699 Msa0181730:intron 40.0%
TTTCCCCTTAAACTGCAGTT+GGG - chr1_4:69972681-69972700 Msa0181730:CDS 40.0%
TAACAGTATTGCAGACCTGT+TGG - chr1_4:69972749-69972768 Msa0181730:intron 40.0%
! TGCAAGTGTTGTGGTAAGTT+AGG - chr1_4:69972791-69972810 Msa0181730:intron 40.0%
GTGGTAAGTTAGGAAGAATG+GGG - chr1_4:69972801-69972820 Msa0181730:intron 40.0%
GAGAAAGACAAAGAAGAAGC+AGG - chr1_4:69973155-69973174 Msa0181730:intron 40.0%
TACTTCTGCTGCCATCATAT+CGG - chr1_4:69973245-69973264 Msa0181730:intron 40.0%
ACAGCAAAGACTTGATGAAG+AGG - chr1_4:69973302-69973321 Msa0181730:intron 40.0%
CAGCAAAGACTTGATGAAGA+GGG - chr1_4:69973303-69973322 Msa0181730:intron 40.0%
GCTTGCTTTCCTAATCTAGA+TGG - chr1_4:69973456-69973475 Msa0181730:five_prime_UTR 40.0%
CATGTGCTACCATCTAGATT+AGG + chr1_4:69973468-69973487 None:intergenic 40.0%
CTAATCTAGATGGTAGCACA+TGG - chr1_4:69973466-69973485 Msa0181730:five_prime_UTR 40.0%
! CACATTGTTTTCGAAAGGTC+CGG + chr1_4:69973529-69973548 None:intergenic 40.0%
! TCGTGCACATTGTTTTCGAA+AGG + chr1_4:69973534-69973553 None:intergenic 40.0%
TTATAGCAGCACAAGCAGTT+AGG - chr1_4:69973601-69973620 Msa0181730:five_prime_UTR 40.0%
GATGAGAGTGGTGATTTGAG+AGG + chr1_4:69971621-69971640 None:intergenic 45.0%
CTCAAATCACCACTCTCATC+AGG - chr1_4:69971621-69971640 Msa0181730:CDS 45.0%
GTAATAGGATCTGCTTGCAG+TGG + chr1_4:69972384-69972403 None:intergenic 45.0%
CTTCCCAACTGCAGTTTAAG+GGG + chr1_4:69972687-69972706 None:intergenic 45.0%
CCTTCCCAACTGCAGTTTAA+GGG + chr1_4:69972688-69972707 None:intergenic 45.0%
CCTTAAACTGCAGTTGGGAA+GGG - chr1_4:69972686-69972705 Msa0181730:CDS 45.0%
! TGTCTCCTGTCGTTTCTTTC+TGG - chr1_4:69972867-69972886 Msa0181730:intron 45.0%
! GTCTCCTGTCGTTTCTTTCT+GGG - chr1_4:69972868-69972887 Msa0181730:intron 45.0%
CAATCCCAGAAAGAAACGAC+AGG + chr1_4:69972875-69972894 None:intergenic 45.0%
GTATTCCTACAGACCTGAGT+GGG - chr1_4:69972913-69972932 Msa0181730:intron 45.0%
!!! GATAGACCACATGCCGTTTT+GGG + chr1_4:69973502-69973521 None:intergenic 45.0%
!! AGATAGACCACATGCCGTTT+TGG + chr1_4:69973503-69973522 None:intergenic 45.0%
ACGAACCACTTTACGAAGCA+GGG - chr1_4:69973550-69973569 Msa0181730:five_prime_UTR 45.0%
CATCTGCATTCTCTGCAATG+TGG + chr1_4:69973632-69973651 None:intergenic 45.0%
CATTGCAGAGAATGCAGATG+AGG - chr1_4:69973632-69973651 Msa0181730:five_prime_UTR 45.0%
CAGAGAATGCAGATGAGGAT+AGG - chr1_4:69973637-69973656 Msa0181730:five_prime_UTR 45.0%
GATGAGAGTGGTGATTTGAG+AGG + chr1_4:69971621-69971640 None:intergenic 45.0%
CTCAAATCACCACTCTCATC+AGG - chr1_4:69971621-69971640 Msa0181730:CDS 45.0%
GTAATAGGATCTGCTTGCAG+TGG + chr1_4:69972384-69972403 None:intergenic 45.0%
CTTCCCAACTGCAGTTTAAG+GGG + chr1_4:69972687-69972706 None:intergenic 45.0%
CCTTCCCAACTGCAGTTTAA+GGG + chr1_4:69972688-69972707 None:intergenic 45.0%
CCTTAAACTGCAGTTGGGAA+GGG - chr1_4:69972686-69972705 Msa0181730:CDS 45.0%
! TGTCTCCTGTCGTTTCTTTC+TGG - chr1_4:69972867-69972886 Msa0181730:intron 45.0%
! GTCTCCTGTCGTTTCTTTCT+GGG - chr1_4:69972868-69972887 Msa0181730:intron 45.0%
CAATCCCAGAAAGAAACGAC+AGG + chr1_4:69972875-69972894 None:intergenic 45.0%
GTATTCCTACAGACCTGAGT+GGG - chr1_4:69972913-69972932 Msa0181730:intron 45.0%
!!! GATAGACCACATGCCGTTTT+GGG + chr1_4:69973502-69973521 None:intergenic 45.0%
!! AGATAGACCACATGCCGTTT+TGG + chr1_4:69973503-69973522 None:intergenic 45.0%
ACGAACCACTTTACGAAGCA+GGG - chr1_4:69973550-69973569 Msa0181730:five_prime_UTR 45.0%
CATCTGCATTCTCTGCAATG+TGG + chr1_4:69973632-69973651 None:intergenic 45.0%
CATTGCAGAGAATGCAGATG+AGG - chr1_4:69973632-69973651 Msa0181730:five_prime_UTR 45.0%
CAGAGAATGCAGATGAGGAT+AGG - chr1_4:69973637-69973656 Msa0181730:five_prime_UTR 45.0%
!!! AATTTGAAAATTTTATATTT+TGG - chr1_4:69971814-69971833 Msa0181730:CDS 5.0%
!!! ATTTGAAAATTTTATATTTT+GGG - chr1_4:69971815-69971834 Msa0181730:CDS 5.0%
!!! AATTTGAAAATTTTATATTT+TGG - chr1_4:69971814-69971833 Msa0181730:CDS 5.0%
!!! ATTTGAAAATTTTATATTTT+GGG - chr1_4:69971815-69971834 Msa0181730:CDS 5.0%
GAGACAAGACCTGATGAGAG+TGG + chr1_4:69971633-69971652 None:intergenic 50.0%
TCTCTCTATGCCATTCCCTC+AGG - chr1_4:69971660-69971679 Msa0181730:CDS 50.0%
CCCTTAAACTGCAGTTGGGA+AGG - chr1_4:69972685-69972704 Msa0181730:CDS 50.0%
CCCTTCCCAACTGCAGTTTA+AGG + chr1_4:69972689-69972708 None:intergenic 50.0%
!!! TGGAGTCGGAGTGTTGGTTT+TGG - chr1_4:69972714-69972733 Msa0181730:intron 50.0%
!!! TGCAGACCTGTTGGCCTTTT+CGG - chr1_4:69972758-69972777 Msa0181730:intron 50.0%
AGTTAGGAAGAATGGGGCCA+AGG - chr1_4:69972807-69972826 Msa0181730:intron 50.0%
GTTAGGAAGAATGGGGCCAA+GGG - chr1_4:69972808-69972827 Msa0181730:intron 50.0%
GCACTCTTAGATCCATCCCT+TGG + chr1_4:69972827-69972846 None:intergenic 50.0%
GGTATTCCTACAGACCTGAG+TGG - chr1_4:69972912-69972931 Msa0181730:intron 50.0%
GAAGCAGGTGAAAGATGAGC+TGG - chr1_4:69973170-69973189 Msa0181730:intron 50.0%
GAGACGGTTGGAAAAAGCTC+TGG - chr1_4:69973221-69973240 Msa0181730:intron 50.0%
TGCTGCCATCATATCGGAAC+TGG - chr1_4:69973251-69973270 Msa0181730:intron 50.0%
!! TGAAGAGGGTGCTGCAATTG+CGG - chr1_4:69973317-69973336 Msa0181730:intron 50.0%
GAGTAGAACATGCAGAGCGA+CGG + chr1_4:69973346-69973365 None:intergenic 50.0%
ATAAGGTTGAGTGCAAGGCC+TGG - chr1_4:69973388-69973407 Msa0181730:intron 50.0%
TAAGGTTGAGTGCAAGGCCT+GGG - chr1_4:69973389-69973408 Msa0181730:intron 50.0%
TGGTCCGTCACTTCCCAAAA+CGG - chr1_4:69973486-69973505 Msa0181730:five_prime_UTR 50.0%
!!! CATGCCGTTTTGGGAAGTGA+CGG + chr1_4:69973493-69973512 None:intergenic 50.0%
TCACTTCCCAAAACGGCATG+TGG - chr1_4:69973493-69973512 Msa0181730:five_prime_UTR 50.0%
CACGAACCACTTTACGAAGC+AGG - chr1_4:69973549-69973568 Msa0181730:five_prime_UTR 50.0%
AACCACTTTACGAAGCAGGG+TGG - chr1_4:69973553-69973572 Msa0181730:five_prime_UTR 50.0%
ACCACTTTACGAAGCAGGGT+GGG - chr1_4:69973554-69973573 Msa0181730:five_prime_UTR 50.0%
GAGACAAGACCTGATGAGAG+TGG + chr1_4:69971633-69971652 None:intergenic 50.0%
TCTCTCTATGCCATTCCCTC+AGG - chr1_4:69971660-69971679 Msa0181730:CDS 50.0%
CCCTTAAACTGCAGTTGGGA+AGG - chr1_4:69972685-69972704 Msa0181730:CDS 50.0%
CCCTTCCCAACTGCAGTTTA+AGG + chr1_4:69972689-69972708 None:intergenic 50.0%
!!! TGGAGTCGGAGTGTTGGTTT+TGG - chr1_4:69972714-69972733 Msa0181730:intron 50.0%
!!! TGCAGACCTGTTGGCCTTTT+CGG - chr1_4:69972758-69972777 Msa0181730:intron 50.0%
AGTTAGGAAGAATGGGGCCA+AGG - chr1_4:69972807-69972826 Msa0181730:intron 50.0%
GTTAGGAAGAATGGGGCCAA+GGG - chr1_4:69972808-69972827 Msa0181730:intron 50.0%
GCACTCTTAGATCCATCCCT+TGG + chr1_4:69972827-69972846 None:intergenic 50.0%
GGTATTCCTACAGACCTGAG+TGG - chr1_4:69972912-69972931 Msa0181730:intron 50.0%
GAAGCAGGTGAAAGATGAGC+TGG - chr1_4:69973170-69973189 Msa0181730:intron 50.0%
GAGACGGTTGGAAAAAGCTC+TGG - chr1_4:69973221-69973240 Msa0181730:intron 50.0%
TGCTGCCATCATATCGGAAC+TGG - chr1_4:69973251-69973270 Msa0181730:intron 50.0%
!! TGAAGAGGGTGCTGCAATTG+CGG - chr1_4:69973317-69973336 Msa0181730:intron 50.0%
GAGTAGAACATGCAGAGCGA+CGG + chr1_4:69973346-69973365 None:intergenic 50.0%
ATAAGGTTGAGTGCAAGGCC+TGG - chr1_4:69973388-69973407 Msa0181730:intron 50.0%
TAAGGTTGAGTGCAAGGCCT+GGG - chr1_4:69973389-69973408 Msa0181730:intron 50.0%
TGGTCCGTCACTTCCCAAAA+CGG - chr1_4:69973486-69973505 Msa0181730:five_prime_UTR 50.0%
!!! CATGCCGTTTTGGGAAGTGA+CGG + chr1_4:69973493-69973512 None:intergenic 50.0%
TCACTTCCCAAAACGGCATG+TGG - chr1_4:69973493-69973512 Msa0181730:five_prime_UTR 50.0%
CACGAACCACTTTACGAAGC+AGG - chr1_4:69973549-69973568 Msa0181730:five_prime_UTR 50.0%
AACCACTTTACGAAGCAGGG+TGG - chr1_4:69973553-69973572 Msa0181730:five_prime_UTR 50.0%
ACCACTTTACGAAGCAGGGT+GGG - chr1_4:69973554-69973573 Msa0181730:five_prime_UTR 50.0%
! TGCAGTTGGGAAGGGTGTAC+TGG - chr1_4:69972694-69972713 Msa0181730:CDS 55.0%
! TGGGAAGGGTGTACTGGAGT+CGG - chr1_4:69972700-69972719 Msa0181730:CDS 55.0%
GTGTACTGGAGTCGGAGTGT+TGG - chr1_4:69972708-69972727 Msa0181730:CDS 55.0%
!!! GCAGACCTGTTGGCCTTTTC+GGG - chr1_4:69972759-69972778 Msa0181730:intron 55.0%
!!! ACCTGTTGGCCTTTTCGGGA+TGG - chr1_4:69972763-69972782 Msa0181730:intron 55.0%
!!! CCTGTTGGCCTTTTCGGGAT+GGG - chr1_4:69972764-69972783 Msa0181730:intron 55.0%
! ATGGGCGAGTGCAAGTGTTG+TGG - chr1_4:69972782-69972801 Msa0181730:intron 55.0%
CTACACCCACTCAGGTCTGT+AGG + chr1_4:69972921-69972940 None:intergenic 55.0%
ACAGACCTGAGTGGGTGTAG+TGG - chr1_4:69972921-69972940 Msa0181730:intron 55.0%
ACCTGAGTGGGTGTAGTGGT+TGG - chr1_4:69972925-69972944 Msa0181730:intron 55.0%
ACCAACCACTACACCCACTC+AGG + chr1_4:69972929-69972948 None:intergenic 55.0%
GGCATGTGGTCTATCTCGTC+CGG - chr1_4:69973507-69973526 Msa0181730:five_prime_UTR 55.0%
TCCCACCCTGCTTCGTAAAG+TGG + chr1_4:69973558-69973577 None:intergenic 55.0%
! TGCAGTTGGGAAGGGTGTAC+TGG - chr1_4:69972694-69972713 Msa0181730:CDS 55.0%
! TGGGAAGGGTGTACTGGAGT+CGG - chr1_4:69972700-69972719 Msa0181730:CDS 55.0%
GTGTACTGGAGTCGGAGTGT+TGG - chr1_4:69972708-69972727 Msa0181730:CDS 55.0%
!!! GCAGACCTGTTGGCCTTTTC+GGG - chr1_4:69972759-69972778 Msa0181730:intron 55.0%
!!! ACCTGTTGGCCTTTTCGGGA+TGG - chr1_4:69972763-69972782 Msa0181730:intron 55.0%
!!! CCTGTTGGCCTTTTCGGGAT+GGG - chr1_4:69972764-69972783 Msa0181730:intron 55.0%
! ATGGGCGAGTGCAAGTGTTG+TGG - chr1_4:69972782-69972801 Msa0181730:intron 55.0%
CTACACCCACTCAGGTCTGT+AGG + chr1_4:69972921-69972940 None:intergenic 55.0%
ACAGACCTGAGTGGGTGTAG+TGG - chr1_4:69972921-69972940 Msa0181730:intron 55.0%
ACCTGAGTGGGTGTAGTGGT+TGG - chr1_4:69972925-69972944 Msa0181730:intron 55.0%
ACCAACCACTACACCCACTC+AGG + chr1_4:69972929-69972948 None:intergenic 55.0%
GGCATGTGGTCTATCTCGTC+CGG - chr1_4:69973507-69973526 Msa0181730:five_prime_UTR 55.0%
TCCCACCCTGCTTCGTAAAG+TGG + chr1_4:69973558-69973577 None:intergenic 55.0%
CCCATCCCGAAAAGGCCAAC+AGG + chr1_4:69972767-69972786 None:intergenic 60.0%
TGCACTCGCCCATCCCGAAA+AGG + chr1_4:69972775-69972794 None:intergenic 60.0%
GGAAGAATGGGGCCAAGGGA+TGG - chr1_4:69972812-69972831 Msa0181730:intron 60.0%
CCCATCCCGAAAAGGCCAAC+AGG + chr1_4:69972767-69972786 None:intergenic 60.0%
TGCACTCGCCCATCCCGAAA+AGG + chr1_4:69972775-69972794 None:intergenic 60.0%
GGAAGAATGGGGCCAAGGGA+TGG - chr1_4:69972812-69972831 Msa0181730:intron 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 69971434 69973671 69971434 ID=Msa0181730;Name=Msa0181730
chr1_4 mRNA 69971434 69973671 69971434 ID=Msa0181730-mRNA-1;Parent=Msa0181730;Name=Msa0181730-mRNA-1;_AED=0.29;_eAED=0.42;_QI=233|0|0.33|0.66|1|1|3|0|206
chr1_4 exon 69971434 69971992 69971434 ID=Msa0181730-mRNA-1:exon:23349;Parent=Msa0181730-mRNA-1
chr1_4 exon 69972681 69972727 69972681 ID=Msa0181730-mRNA-1:exon:23348;Parent=Msa0181730-mRNA-1
chr1_4 exon 69973424 69973671 69973424 ID=Msa0181730-mRNA-1:exon:23347;Parent=Msa0181730-mRNA-1
chr1_4 five_prime_UTR 69973439 69973671 69973439 ID=Msa0181730-mRNA-1:five_prime_utr;Parent=Msa0181730-mRNA-1
chr1_4 CDS 69973424 69973438 69973424 ID=Msa0181730-mRNA-1:cds;Parent=Msa0181730-mRNA-1
chr1_4 CDS 69972681 69972727 69972681 ID=Msa0181730-mRNA-1:cds;Parent=Msa0181730-mRNA-1
chr1_4 CDS 69971434 69971992 69971434 ID=Msa0181730-mRNA-1:cds;Parent=Msa0181730-mRNA-1
Gene Sequence

>Msa0181730

ATGCCATTCCCTCAGTTACCACTGCAAGCAGATCCTATTACAATAATATACATGTTAAGTAGTTCAATGGATAACATGGCATGTAATAAAGGGCAACAACATGTGAGAAAGACAAAGAAGAAGCAGGTGAAAGATGAGCTGGATCGTCTCAAACAAGCTGAAAAGAAAAAGAGACGGTTGGAAAAAGCTCTGGCTACTTCTGCTGCCATCATATCGGAACTGGAAAAAAAGAAACAGAAAAAGAAAGAAGAACAGCAAAGACTTGATGAAGAGGGTGCTGCAATTGCGGAAGCCGTCGCTCTGCATGTTCTACTCGATGAAGACTCAGATGATTCATATAAGGTTGAGTGCAAGGCCTGGGATGATTATAATAATAATCTTGATTTCTTCATGGGTGGGAAAAGAGCTTGCTTTCCTAATCTAGATGGTAGCACATGGTCCGTCACTTCCCAAAACGGCATGTGGTCTATCTCGTCCGGACCTTTCGAAAACAATGTGCACGAACCACTTTACGAAGCAGGGTGGGATCCTACAAGATTCTCTGTTGATCTTATAGCAGCACAAGCAGTTAGGTCACTCCACATTGCAGAGAATGCAGATGAGGATAGGATTCTCTTCTAA
Protein sequence

>Msa0181730

MPFPQLPLQADPITIIYMLSSSMDNMACNKGQQHVRKTKKKQVKDELDRLKQAEKKKRRLEKALATSAAIISELEKKKQKKKEEQQRLDEEGAAIAEAVALHVLLDEDSDDSYKVECKAWDDYNNNLDFFMGGKRACFPNLDGSTWSVTSQNGMWSISSGPFENNVHEPLYEAGWDPTRFSVDLIAAQAVRSLHIAENADEDRILF*