AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0937330


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0937330 MtrunA17_Chr6g0453281 98.312 237 4 0 1 237 1 237 5.33e-174 478
Msa0937330 MtrunA17_Chr7g0217561 61.603 237 71 3 1 237 1 217 3.74e-87 257
Msa0937330 MtrunA17_Chr2g0287451 39.516 124 62 4 114 234 79 192 2.35e-14 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0937330 AT3G01170.1 55.000 240 80 5 1 237 1 215 4.89e-83 247
Msa0937330 AT5G15260.2 55.691 246 88 9 1 237 1 234 2.06e-74 226
Msa0937330 AT5G15260.1 55.691 246 88 9 1 237 1 234 2.06e-74 226
Msa0937330 AT3G06180.1 40.000 165 94 3 75 237 66 227 5.97e-23 93.6
Msa0937330 AT5G19025.1 51.515 99 45 2 139 237 150 245 1.55e-22 93.2
Msa0937330 AT5G19025.2 50.526 95 45 1 139 233 150 242 3.33e-22 92.4
Msa0937330 AT3G01170.2 43.220 118 48 3 1 116 1 101 2.22e-21 87.4

Find 45 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTATATTTATATATCTAT+TGG 0.238022 6_3:-8531265 Msa0937330:CDS
GTCATTTCAGATTATGAGTA+TGG 0.244984 6_3:-8531323 Msa0937330:CDS
TAACAGAATTTGATAAACTA+TGG 0.289659 6_3:+8531461 None:intergenic
TGTTGGTAGGTTAGAAGTTC+CGG 0.289963 6_3:-8530948 Msa0937330:CDS
AAGAAGATGGCACCGATTAA+TGG 0.325801 6_3:-8531010 Msa0937330:CDS
CCAATAGATATATAAATATA+AGG 0.341548 6_3:+8531265 None:intergenic
ATGGCGAATTCGATCGAATT+CGG 0.378268 6_3:-8531538 Msa0937330:CDS
TTTGTGCTGCATTGGCTACA+TGG 0.387252 6_3:-8531231 Msa0937330:CDS
TTCTTCAAACCCTTACAATT+CGG 0.389737 6_3:+8531172 None:intergenic
CGAAGTTTCTTCGTCGGTCC+CGG 0.401109 6_3:+8530929 None:intergenic
GTTGGTAGGTTAGAAGTTCC+GGG 0.404512 6_3:-8530947 Msa0937330:CDS
ATCACTAAAAGTTCAACATA+AGG 0.412821 6_3:+8531349 None:intergenic
CTAATCAATTTCAAAGGAAT+AGG 0.420647 6_3:-8531489 Msa0937330:CDS
ATGTGGTAATCCGAATTGTA+AGG 0.420695 6_3:-8531182 Msa0937330:CDS
GATGGCGGTGTTTGTAAGAA+GGG 0.429289 6_3:-8531082 Msa0937330:CDS
AGCACAGCTCTTCCATTAAT+CGG 0.433317 6_3:+8530998 None:intergenic
ATTGCTGTTGTTATTGCTTG+TGG 0.436635 6_3:-8531385 Msa0937330:CDS
AGATGGCGGTGTTTGTAAGA+AGG 0.445969 6_3:-8531083 Msa0937330:CDS
CTGTGCTTGTGCTGAGAGCT+AGG 0.461752 6_3:-8530982 Msa0937330:CDS
GGTGTGGTTGTTCTGTTGGT+AGG 0.475981 6_3:-8530961 Msa0937330:CDS
GCTAGGTGTGGTTGTTCTGT+TGG 0.477365 6_3:-8530965 Msa0937330:CDS
TTCGCCATGGATGATTGAAC+TGG 0.483771 6_3:+8531553 None:intergenic
ATCTTCCGAAGTTTCTTCGT+CGG 0.488611 6_3:+8530923 None:intergenic
TCACTAAAAGTTCAACATAA+GGG 0.493997 6_3:+8531350 None:intergenic
TTGTGCTGCATTGGCTACAT+GGG 0.507240 6_3:-8531230 Msa0937330:CDS
ATCAATTTCAAAGGAATAGG+AGG 0.510788 6_3:-8531486 Msa0937330:CDS
AAGTGTTGTTTGTGCTGCAT+TGG 0.523765 6_3:-8531239 Msa0937330:CDS
ACTTCGGAAGATCAAGAAGT+AGG 0.555452 6_3:-8530912 None:intergenic
AGCAAACTAATCAATTTCAA+AGG 0.564372 6_3:-8531495 Msa0937330:CDS
TGTGGTAATCCGAATTGTAA+GGG 0.569597 6_3:-8531181 Msa0937330:CDS
TGAAGCGGAGCTTAAGAAGA+TGG 0.571103 6_3:-8531023 Msa0937330:CDS
TACAAAGAGAGATTGAGCTA+TGG 0.571615 6_3:+8531593 None:intergenic
ATTGCCGCGTGATCATCATA+AGG 0.580739 6_3:-8531050 Msa0937330:CDS
ACCTCCAGTTCAATCATCCA+TGG 0.585489 6_3:-8531557 Msa0937330:CDS
CCGCCATCTTTCACAAAAGA+CGG 0.592301 6_3:+8531097 None:intergenic
GTTTGCACAAGTAACAAATG+TGG 0.622560 6_3:-8531199 Msa0937330:CDS
TCATCATAAGGAACTTGAAG+CGG 0.636755 6_3:-8531038 Msa0937330:CDS
CGGGACCGACGAAGAAACTT+CGG 0.636873 6_3:-8530928 Msa0937330:CDS
GCCATGGATGATTGAACTGG+AGG 0.647249 6_3:+8531556 None:intergenic
GAATTCGATCGAATTCGCCA+TGG 0.649026 6_3:+8531540 None:intergenic
TGTGCTGCATTGGCTACATG+GGG 0.654737 6_3:-8531229 Msa0937330:CDS
CTTGTGCTGAGAGCTAGGTG+TGG 0.697858 6_3:-8530977 Msa0937330:CDS
GCTGATCTAGATCGATCACA+AGG 0.698983 6_3:+8531430 None:intergenic
GATCTAGATCGATCACAAGG+TGG 0.717259 6_3:+8531433 None:intergenic
AGTTCCTTATGATGATCACG+CGG 0.724998 6_3:+8531046 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CCAATAGATATATAAATATA+AGG + chr6_3:8531255-8531274 None:intergenic 15.0%
!! CCTTATATTTATATATCTAT+TGG - chr6_3:8531252-8531271 Msa0937330:CDS 15.0%
!! TAACAGAATTTGATAAACTA+TGG + chr6_3:8531059-8531078 None:intergenic 20.0%
! AGCAAACTAATCAATTTCAA+AGG - chr6_3:8531022-8531041 Msa0937330:CDS 25.0%
! CTAATCAATTTCAAAGGAAT+AGG - chr6_3:8531028-8531047 Msa0937330:CDS 25.0%
! TCACTAAAAGTTCAACATAA+GGG + chr6_3:8531170-8531189 None:intergenic 25.0%
! ATCACTAAAAGTTCAACATA+AGG + chr6_3:8531171-8531190 None:intergenic 25.0%
!!! TATGGTGTGTTTTTTGATTA+TGG - chr6_3:8531212-8531231 Msa0937330:CDS 25.0%
ATCAATTTCAAAGGAATAGG+AGG - chr6_3:8531031-8531050 Msa0937330:CDS 30.0%
GTCATTTCAGATTATGAGTA+TGG - chr6_3:8531194-8531213 Msa0937330:CDS 30.0%
TTCTTCAAACCCTTACAATT+CGG + chr6_3:8531348-8531367 None:intergenic 30.0%
TACAAAGAGAGATTGAGCTA+TGG + chr6_3:8530927-8530946 None:intergenic 35.0%
! ATTGCTGTTGTTATTGCTTG+TGG - chr6_3:8531132-8531151 Msa0937330:CDS 35.0%
GTTTGCACAAGTAACAAATG+TGG - chr6_3:8531318-8531337 Msa0937330:CDS 35.0%
ATGTGGTAATCCGAATTGTA+AGG - chr6_3:8531335-8531354 Msa0937330:CDS 35.0%
TGTGGTAATCCGAATTGTAA+GGG - chr6_3:8531336-8531355 Msa0937330:CDS 35.0%
TCATCATAAGGAACTTGAAG+CGG - chr6_3:8531479-8531498 Msa0937330:CDS 35.0%
ATGGCGAATTCGATCGAATT+CGG - chr6_3:8530979-8530998 Msa0937330:CDS 40.0%
!! AAGTGTTGTTTGTGCTGCAT+TGG - chr6_3:8531278-8531297 Msa0937330:CDS 40.0%
AGTTCCTTATGATGATCACG+CGG + chr6_3:8531474-8531493 None:intergenic 40.0%
!! AAGAAGATGGCACCGATTAA+TGG - chr6_3:8531507-8531526 Msa0937330:CDS 40.0%
AGCACAGCTCTTCCATTAAT+CGG + chr6_3:8531522-8531541 None:intergenic 40.0%
! TGTTGGTAGGTTAGAAGTTC+CGG - chr6_3:8531569-8531588 Msa0937330:CDS 40.0%
ATCTTCCGAAGTTTCTTCGT+CGG + chr6_3:8531597-8531616 None:intergenic 40.0%
ACCTCCAGTTCAATCATCCA+TGG - chr6_3:8530960-8530979 Msa0937330:CDS 45.0%
TTCGCCATGGATGATTGAAC+TGG + chr6_3:8530967-8530986 None:intergenic 45.0%
GAATTCGATCGAATTCGCCA+TGG + chr6_3:8530980-8530999 None:intergenic 45.0%
GATCTAGATCGATCACAAGG+TGG + chr6_3:8531087-8531106 None:intergenic 45.0%
GCTGATCTAGATCGATCACA+AGG + chr6_3:8531090-8531109 None:intergenic 45.0%
!! TTTGTGCTGCATTGGCTACA+TGG - chr6_3:8531286-8531305 Msa0937330:CDS 45.0%
!! TTGTGCTGCATTGGCTACAT+GGG - chr6_3:8531287-8531306 Msa0937330:CDS 45.0%
! TCGCCGTCTTTTGTGAAAGA+TGG - chr6_3:8531417-8531436 Msa0937330:CDS 45.0%
CCGCCATCTTTCACAAAAGA+CGG + chr6_3:8531423-8531442 None:intergenic 45.0%
! CCGTCTTTTGTGAAAGATGG+CGG - chr6_3:8531420-8531439 Msa0937330:CDS 45.0%
! AGATGGCGGTGTTTGTAAGA+AGG - chr6_3:8531434-8531453 Msa0937330:CDS 45.0%
! GATGGCGGTGTTTGTAAGAA+GGG - chr6_3:8531435-8531454 Msa0937330:CDS 45.0%
ATTGCCGCGTGATCATCATA+AGG - chr6_3:8531467-8531486 Msa0937330:CDS 45.0%
TGAAGCGGAGCTTAAGAAGA+TGG - chr6_3:8531494-8531513 Msa0937330:CDS 45.0%
! GTTGGTAGGTTAGAAGTTCC+GGG - chr6_3:8531570-8531589 Msa0937330:CDS 45.0%
GCCATGGATGATTGAACTGG+AGG + chr6_3:8530964-8530983 None:intergenic 50.0%
!! TGTGCTGCATTGGCTACATG+GGG - chr6_3:8531288-8531307 Msa0937330:CDS 50.0%
GCTAGGTGTGGTTGTTCTGT+TGG - chr6_3:8531552-8531571 Msa0937330:CDS 50.0%
GGTGTGGTTGTTCTGTTGGT+AGG - chr6_3:8531556-8531575 Msa0937330:CDS 50.0%
CTGTGCTTGTGCTGAGAGCT+AGG - chr6_3:8531535-8531554 Msa0937330:CDS 55.0%
CTTGTGCTGAGAGCTAGGTG+TGG - chr6_3:8531540-8531559 Msa0937330:CDS 55.0%
CGAAGTTTCTTCGTCGGTCC+CGG + chr6_3:8531591-8531610 None:intergenic 55.0%
CGGGACCGACGAAGAAACTT+CGG - chr6_3:8531589-8531608 Msa0937330:CDS 55.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 8530913 8531626 8530913 ID=Msa0937330;Name=Msa0937330
chr6_3 mRNA 8530913 8531626 8530913 ID=Msa0937330-mRNA-1;Parent=Msa0937330;Name=Msa0937330-mRNA-1;_AED=0.00;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|237
chr6_3 exon 8530913 8531626 8530913 ID=Msa0937330-mRNA-1:exon:2748;Parent=Msa0937330-mRNA-1
chr6_3 CDS 8530913 8531626 8530913 ID=Msa0937330-mRNA-1:cds;Parent=Msa0937330-mRNA-1
Gene Sequence

>Msa0937330

ATGGTCTATTTCCATAGCTCAATCTCTCTTTGTAAGTCAGTTGATCAACCTCCAGTTCAATCATCCATGGCGAATTCGATCGAATTCGGATCGAAATGTTCGAAATCTAAGCAAACTAATCAATTTCAAAGGAATAGGAGGAACCATAGTTTATCAAATTCTGTTAAGATTCCACCTTGTGATCGATCTAGATCAGCTATGATTGATGTTGTTATGTTCATTGCTGTTGTTATTGCTTGTGGATTTTTGTTTTTCCCTTATGTTGAACTTTTAGTGATTAAGTCATTTCAGATTATGAGTATGGTGTGTTTTTTGATTATGGAAGAAGTTTCTTATGCACCTTATATTTATATATCTATTGGAATAAGTGTTGTTTGTGCTGCATTGGCTACATGGGGTGTTATTGTTTGCACAAGTAACAAATGTGGTAATCCGAATTGTAAGGGTTTGAAGAAAGCTGCAGAATTTGATATTCAGTTAGAGACTGAAGATTGTGTGAAGAATTCGCCGTCTTTTGTGAAAGATGGCGGTGTTTGTAAGAAGGGTTTGTTTGAATTGCCGCGTGATCATCATAAGGAACTTGAAGCGGAGCTTAAGAAGATGGCACCGATTAATGGAAGAGCTGTGCTTGTGCTGAGAGCTAGGTGTGGTTGTTCTGTTGGTAGGTTAGAAGTTCCGGGACCGACGAAGAAACTTCGGAAGATCAAGAAGTAG

Protein sequence

>Msa0937330

MVYFHSSISLCKSVDQPPVQSSMANSIEFGSKCSKSKQTNQFQRNRRNHSLSNSVKIPPCDRSRSAMIDVVMFIAVVIACGFLFFPYVELLVIKSFQIMSMVCFLIMEEVSYAPYIYISIGISVVCAALATWGVIVCTSNKCGNPNCKGLKKAAEFDIQLETEDCVKNSPSFVKDGGVCKKGLFELPRDHHKELEAELKKMAPINGRAVLVLRARCGCSVGRLEVPGPTKKLRKIKK*