AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0563650

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0563650 MtrunA17_Chr8g0377461 100.000 68 0 0 1 68 1 68 5.48e-44 135
Msa0563650 MtrunA17_Chr2g0290301 54.545 66 23 1 1 66 1 59 5.56e-19 72.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0563650 AT1G27350.1 88.235 68 8 0 1 68 1 68 1.94e-38 122
Msa0563650 AT1G27330.1 88.235 68 8 0 1 68 1 68 1.94e-38 122

Find 40 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATTAAATTCAACAAAATT+AGG 0.206186 4_1:-64091559 Msa0563650:five_prime_UTR
TCAACAAAATTAGGCATATT+AGG 0.209880 4_1:-64091550 Msa0563650:five_prime_UTR
CCTTTCTTAACAGTTGTTTC+TGG 0.223249 4_1:+64090856 None:intergenic
ATGATTGATTTGTAGGATAA+TGG 0.270299 4_1:+64091352 None:intergenic
TTCTTCGTCTTTGTTGTCAT+TGG 0.272950 4_1:-64090799 Msa0563650:CDS
ACGAACCATGATTGATTTGT+AGG 0.306992 4_1:+64091345 None:intergenic
ACGAAGAAACCAAGCAAAAC+GGG 0.342450 4_1:+64090814 None:intergenic
ATCAAAACATGAACAGGAAC+TGG 0.348130 4_1:+64089645 None:intergenic
CAGCTCTCTTTCAGATAATC+AGG 0.365308 4_1:-64089780 Msa0563650:CDS
CCAAGCAAAACGGGGCCAAC+AGG 0.391773 4_1:+64090823 None:intergenic
GGGATTAGTCATCGTATAAA+TGG 0.392005 4_1:-64089493 Msa0563650:three_prime_UTR
AACTTCATCAAAACATGAAC+AGG 0.410889 4_1:+64089639 None:intergenic
GACGAAGAAACCAAGCAAAA+CGG 0.413008 4_1:+64090813 None:intergenic
TGAACGGTGATCGTTGTTAC+CGG 0.416731 4_1:+64091485 None:intergenic
CATCAAAGCGCCTTGCAGTT+AGG 0.424154 4_1:-64090924 Msa0563650:CDS
GGCATAATGGAGCTTTGAGT+TGG 0.445961 4_1:-64089737 Msa0563650:exon
CGAGTGGAGGCATGGCATAA+TGG 0.448620 4_1:-64089750 Msa0563650:exon
AAAAGTTGGAAATAAATAAC+CGG 0.474202 4_1:-64091504 Msa0563650:five_prime_UTR
TATGTAAAATTAAACAAAAG+AGG 0.501977 4_1:+64089578 None:intergenic
AATTAGGCATATTAGGTAAG+TGG 0.504893 4_1:-64091543 Msa0563650:five_prime_UTR
CCAGAAACAACTGTTAAGAA+AGG 0.524644 4_1:-64090856 Msa0563650:CDS
ATTATCCTACAAATCAATCA+TGG 0.554540 4_1:-64091350 Msa0563650:intron
AAAGGAAATGACTATCCTGT+TGG 0.571369 4_1:-64090838 Msa0563650:CDS
GACAGCAACGAGTGGAGGCA+TGG 0.571659 4_1:-64089758 Msa0563650:CDS
AAAGCGCCTTGCAGTTAGGA+AGG 0.575086 4_1:-64090920 Msa0563650:CDS
ACCTCGGAGAGATTCACTGC+CGG 0.593695 4_1:-64091436 Msa0563650:five_prime_UTR
TTTGTAGGATAATGGATCGC+CGG 0.595314 4_1:+64091360 None:intergenic
TCAAAACATGAACAGGAACT+GGG 0.600163 4_1:+64089646 None:intergenic
ATCAGGACAGCAACGAGTGG+AGG 0.608196 4_1:-64089763 Msa0563650:CDS
CGAAGAAACCAAGCAAAACG+GGG 0.617394 4_1:+64090815 None:intergenic
GAAATCTCTAAATAAAACCT+CGG 0.627274 4_1:-64091452 Msa0563650:five_prime_UTR
TCCGGCAGTGAATCTCTCCG+AGG 0.637520 4_1:+64091435 None:intergenic
TCTCAACCTTCCTAACTGCA+AGG 0.647474 4_1:+64090914 None:intergenic
GATTTCTGAATATCTGTGAA+CGG 0.648636 4_1:+64091469 None:intergenic
ATTGATGCTGTGTAATCGTG+AGG 0.656067 4_1:-64089514 Msa0563650:three_prime_UTR
ATAATCAGGACAGCAACGAG+TGG 0.658965 4_1:-64089766 Msa0563650:CDS
GTAGGATAATGGATCGCCGG+TGG 0.665340 4_1:+64091363 None:intergenic
AACGCCGGAAAATTGAAAGA+CGG 0.693395 4_1:+64091398 None:intergenic
GCCGGAAAATTGAAAGACGG+TGG 0.699767 4_1:+64091401 None:intergenic
TTGATGCTGTGTAATCGTGA+GGG 0.713573 4_1:-64089513 Msa0563650:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAGAAATTTATCAAAATT+AGG - chr4_1:64089905-64089924 Msa0563650:intron 15.0%
!! AAAAATTAGTAAAACACATA+AGG + chr4_1:64090298-64090317 None:intergenic 15.0%
!! CAATATTTCAAAAATTGAAT+CGG + chr4_1:64090548-64090567 None:intergenic 15.0%
!!! AATTTTTGAAATATTGTTGT+GGG - chr4_1:64090552-64090571 Msa0563650:intron 15.0%
!!! TATATTAATGTGTTTGAAAT+TGG + chr4_1:64090719-64090738 None:intergenic 15.0%
!!! ATAATTTTCAATTCAATTCA+AGG + chr4_1:64090802-64090821 None:intergenic 15.0%
!! TATGTAAAATTAAACAAAAG+AGG + chr4_1:64091464-64091483 None:intergenic 15.0%
!! ATCAGAAATTTATCAAAATT+AGG - chr4_1:64089905-64089924 Msa0563650:intron 15.0%
!! AAAAATTAGTAAAACACATA+AGG + chr4_1:64090298-64090317 None:intergenic 15.0%
!! CAATATTTCAAAAATTGAAT+CGG + chr4_1:64090548-64090567 None:intergenic 15.0%
!!! AATTTTTGAAATATTGTTGT+GGG - chr4_1:64090552-64090571 Msa0563650:intron 15.0%
!!! TATATTAATGTGTTTGAAAT+TGG + chr4_1:64090719-64090738 None:intergenic 15.0%
!!! ATAATTTTCAATTCAATTCA+AGG + chr4_1:64090802-64090821 None:intergenic 15.0%
!! TATGTAAAATTAAACAAAAG+AGG + chr4_1:64091464-64091483 None:intergenic 15.0%
!! AAAAGTTGGAAATAAATAAC+CGG - chr4_1:64089535-64089554 Msa0563650:three_prime_UTR 20.0%
!! TATTGTTTGTGTGATTTATT+TGG - chr4_1:64090367-64090386 Msa0563650:intron 20.0%
!!! CAATTTTTGAAATATTGTTG+TGG - chr4_1:64090551-64090570 Msa0563650:intron 20.0%
!!! ATTTTTGAAATATTGTTGTG+GGG - chr4_1:64090553-64090572 Msa0563650:intron 20.0%
!!! AAGAAATTTTCGTTTTTGTT+TGG + chr4_1:64090646-64090665 None:intergenic 20.0%
!!! GTAGTTTCATTATCATTTTA+AGG - chr4_1:64091069-64091088 Msa0563650:intron 20.0%
!! AAAAGTTGGAAATAAATAAC+CGG - chr4_1:64089535-64089554 Msa0563650:three_prime_UTR 20.0%
!! TATTGTTTGTGTGATTTATT+TGG - chr4_1:64090367-64090386 Msa0563650:intron 20.0%
!!! CAATTTTTGAAATATTGTTG+TGG - chr4_1:64090551-64090570 Msa0563650:intron 20.0%
!!! ATTTTTGAAATATTGTTGTG+GGG - chr4_1:64090553-64090572 Msa0563650:intron 20.0%
!!! AAGAAATTTTCGTTTTTGTT+TGG + chr4_1:64090646-64090665 None:intergenic 20.0%
!!! GTAGTTTCATTATCATTTTA+AGG - chr4_1:64091069-64091088 Msa0563650:intron 20.0%
! TCAACAAAATTAGGCATATT+AGG - chr4_1:64089489-64089508 Msa0563650:three_prime_UTR 25.0%
! GAAATCTCTAAATAAAACCT+CGG - chr4_1:64089587-64089606 Msa0563650:three_prime_UTR 25.0%
!!! ATGATTGATTTGTAGGATAA+TGG + chr4_1:64089690-64089709 None:intergenic 25.0%
! ATTATCCTACAAATCAATCA+TGG - chr4_1:64089689-64089708 Msa0563650:three_prime_UTR 25.0%
!!! TTACATTGATTTTCAGCTTA+TGG - chr4_1:64089961-64089980 Msa0563650:intron 25.0%
!!! TGTTGAATTTTGATTGAACT+TGG - chr4_1:64090027-64090046 Msa0563650:intron 25.0%
! TGTTGAATGAAATTGAGATT+TGG - chr4_1:64090058-64090077 Msa0563650:intron 25.0%
!!! GAAATTGAGATTTGGAATTT+TGG - chr4_1:64090066-64090085 Msa0563650:intron 25.0%
! GAGAAAAATATCACAAAAAG+AGG - chr4_1:64090153-64090172 Msa0563650:intron 25.0%
! AATTAGTAAAACACATAAGG+AGG + chr4_1:64090295-64090314 None:intergenic 25.0%
! ACTGAACCAATAGTTAAAAT+CGG - chr4_1:64090417-64090436 Msa0563650:intron 25.0%
! CTGAGTACATAATTTATTCT+CGG - chr4_1:64090775-64090794 Msa0563650:intron 25.0%
! TGAGTACATAATTTATTCTC+GGG - chr4_1:64090776-64090795 Msa0563650:intron 25.0%
! GTATATCAAACATTTGATCA+CGG + chr4_1:64090985-64091004 None:intergenic 25.0%
!! GTATAGTCATATTTTCTACA+GGG + chr4_1:64091349-64091368 None:intergenic 25.0%
!! AGTATAGTCATATTTTCTAC+AGG + chr4_1:64091350-64091369 None:intergenic 25.0%
!!! CATGTTTTGATGAAGTTTTA+AGG - chr4_1:64091406-64091425 Msa0563650:five_prime_UTR 25.0%
! TCAACAAAATTAGGCATATT+AGG - chr4_1:64089489-64089508 Msa0563650:three_prime_UTR 25.0%
! GAAATCTCTAAATAAAACCT+CGG - chr4_1:64089587-64089606 Msa0563650:three_prime_UTR 25.0%
!!! ATGATTGATTTGTAGGATAA+TGG + chr4_1:64089690-64089709 None:intergenic 25.0%
! ATTATCCTACAAATCAATCA+TGG - chr4_1:64089689-64089708 Msa0563650:three_prime_UTR 25.0%
!!! TTACATTGATTTTCAGCTTA+TGG - chr4_1:64089961-64089980 Msa0563650:intron 25.0%
!!! TGTTGAATTTTGATTGAACT+TGG - chr4_1:64090027-64090046 Msa0563650:intron 25.0%
! TGTTGAATGAAATTGAGATT+TGG - chr4_1:64090058-64090077 Msa0563650:intron 25.0%
!!! GAAATTGAGATTTGGAATTT+TGG - chr4_1:64090066-64090085 Msa0563650:intron 25.0%
! GAGAAAAATATCACAAAAAG+AGG - chr4_1:64090153-64090172 Msa0563650:intron 25.0%
! AATTAGTAAAACACATAAGG+AGG + chr4_1:64090295-64090314 None:intergenic 25.0%
! ACTGAACCAATAGTTAAAAT+CGG - chr4_1:64090417-64090436 Msa0563650:intron 25.0%
! CTGAGTACATAATTTATTCT+CGG - chr4_1:64090775-64090794 Msa0563650:intron 25.0%
! TGAGTACATAATTTATTCTC+GGG - chr4_1:64090776-64090795 Msa0563650:intron 25.0%
! GTATATCAAACATTTGATCA+CGG + chr4_1:64090985-64091004 None:intergenic 25.0%
!! GTATAGTCATATTTTCTACA+GGG + chr4_1:64091349-64091368 None:intergenic 25.0%
!! AGTATAGTCATATTTTCTAC+AGG + chr4_1:64091350-64091369 None:intergenic 25.0%
!!! CATGTTTTGATGAAGTTTTA+AGG - chr4_1:64091406-64091425 Msa0563650:five_prime_UTR 25.0%
AATTAGGCATATTAGGTAAG+TGG - chr4_1:64089496-64089515 Msa0563650:three_prime_UTR 30.0%
GATTTCTGAATATCTGTGAA+CGG + chr4_1:64089573-64089592 None:intergenic 30.0%
! GTCAAATTTTATGTAGCGTA+TGG - chr4_1:64089927-64089946 Msa0563650:intron 30.0%
!! ATTGAGATTTGGAATTTTGG+TGG - chr4_1:64090069-64090088 Msa0563650:intron 30.0%
TCCTAATTCTTGTGATGAAT+TGG - chr4_1:64090337-64090356 Msa0563650:intron 30.0%
CCTAATTCTTGTGATGAATT+GGG - chr4_1:64090338-64090357 Msa0563650:intron 30.0%
AATTAACAATAGTTCAGCTC+TGG + chr4_1:64090448-64090467 None:intergenic 30.0%
GAGCTGAACTATTGTTAATT+CGG - chr4_1:64090448-64090467 Msa0563650:intron 30.0%
TGTTAATTCGGATATTTGAG+AGG - chr4_1:64090460-64090479 Msa0563650:intron 30.0%
AATATTGTTGTGGGGTTAAT+AGG - chr4_1:64090561-64090580 Msa0563650:intron 30.0%
! TTGTACTGACCTATATTACA+GGG + chr4_1:64090593-64090612 None:intergenic 30.0%
! TTTGTACTGACCTATATTAC+AGG + chr4_1:64090594-64090613 None:intergenic 30.0%
CGAGAATAAATTATGTACTC+AGG + chr4_1:64090777-64090796 None:intergenic 30.0%
AATCGTATTATAATGCAGAG+AGG - chr4_1:64090902-64090921 Msa0563650:CDS 30.0%
ATCGTATTATAATGCAGAGA+GGG - chr4_1:64090903-64090922 Msa0563650:CDS 30.0%
TTAACAATTCCCTATGGAAT+TGG - chr4_1:64090955-64090974 Msa0563650:intron 30.0%
ATGTTTGATATACCTTCTCA+AGG - chr4_1:64090992-64091011 Msa0563650:intron 30.0%
TATAGCTCATGAAAATGCTA+TGG + chr4_1:64091129-64091148 None:intergenic 30.0%
ATGAAAATGCTATCGAAGAA+AGG + chr4_1:64091156-64091175 None:intergenic 30.0%
AACTTCATCAAAACATGAAC+AGG + chr4_1:64091403-64091422 None:intergenic 30.0%
AATTAGGCATATTAGGTAAG+TGG - chr4_1:64089496-64089515 Msa0563650:three_prime_UTR 30.0%
GATTTCTGAATATCTGTGAA+CGG + chr4_1:64089573-64089592 None:intergenic 30.0%
! GTCAAATTTTATGTAGCGTA+TGG - chr4_1:64089927-64089946 Msa0563650:intron 30.0%
!! ATTGAGATTTGGAATTTTGG+TGG - chr4_1:64090069-64090088 Msa0563650:intron 30.0%
TCCTAATTCTTGTGATGAAT+TGG - chr4_1:64090337-64090356 Msa0563650:intron 30.0%
CCTAATTCTTGTGATGAATT+GGG - chr4_1:64090338-64090357 Msa0563650:intron 30.0%
AATTAACAATAGTTCAGCTC+TGG + chr4_1:64090448-64090467 None:intergenic 30.0%
GAGCTGAACTATTGTTAATT+CGG - chr4_1:64090448-64090467 Msa0563650:intron 30.0%
TGTTAATTCGGATATTTGAG+AGG - chr4_1:64090460-64090479 Msa0563650:intron 30.0%
AATATTGTTGTGGGGTTAAT+AGG - chr4_1:64090561-64090580 Msa0563650:intron 30.0%
! TTGTACTGACCTATATTACA+GGG + chr4_1:64090593-64090612 None:intergenic 30.0%
! TTTGTACTGACCTATATTAC+AGG + chr4_1:64090594-64090613 None:intergenic 30.0%
CGAGAATAAATTATGTACTC+AGG + chr4_1:64090777-64090796 None:intergenic 30.0%
AATCGTATTATAATGCAGAG+AGG - chr4_1:64090902-64090921 Msa0563650:CDS 30.0%
ATCGTATTATAATGCAGAGA+GGG - chr4_1:64090903-64090922 Msa0563650:CDS 30.0%
TTAACAATTCCCTATGGAAT+TGG - chr4_1:64090955-64090974 Msa0563650:intron 30.0%
ATGTTTGATATACCTTCTCA+AGG - chr4_1:64090992-64091011 Msa0563650:intron 30.0%
TATAGCTCATGAAAATGCTA+TGG + chr4_1:64091129-64091148 None:intergenic 30.0%
ATGAAAATGCTATCGAAGAA+AGG + chr4_1:64091156-64091175 None:intergenic 30.0%
AACTTCATCAAAACATGAAC+AGG + chr4_1:64091403-64091422 None:intergenic 30.0%
!! ACGAACCATGATTGATTTGT+AGG + chr4_1:64089697-64089716 None:intergenic 35.0%
GAATGCAAGAAATAGAAGAG+AGG + chr4_1:64089726-64089745 None:intergenic 35.0%
! CCTTTCTTAACAGTTGTTTC+TGG + chr4_1:64090186-64090205 None:intergenic 35.0%
CCAGAAACAACTGTTAAGAA+AGG - chr4_1:64090183-64090202 Msa0563650:intron 35.0%
AAAGGAAATGACTATCCTGT+TGG - chr4_1:64090201-64090220 Msa0563650:intron 35.0%
! TTCTTCGTCTTTGTTGTCAT+TGG - chr4_1:64090240-64090259 Msa0563650:intron 35.0%
TAAGGAGGACAACATTGAAA+GGG + chr4_1:64090280-64090299 None:intergenic 35.0%
ATAAGGAGGACAACATTGAA+AGG + chr4_1:64090281-64090300 None:intergenic 35.0%
CCCAATTCATCACAAGAATT+AGG + chr4_1:64090341-64090360 None:intergenic 35.0%
!!! GCTCAACCGATTTTAACTAT+TGG + chr4_1:64090426-64090445 None:intergenic 35.0%
!! GGTTCAATCGTTGTTTTGTT+TGG - chr4_1:64090481-64090500 Msa0563650:intron 35.0%
TCAAGCTGTTGAACTATTTG+AGG - chr4_1:64090505-64090524 Msa0563650:intron 35.0%
! TTGAACTATTTGAGGTCTTG+TGG - chr4_1:64090513-64090532 Msa0563650:intron 35.0%
AGGTCTTTACCCTGTAATAT+AGG - chr4_1:64090581-64090600 Msa0563650:intron 35.0%
!!! GTTTTGATTTACCCCCTAAT+AGG - chr4_1:64090620-64090639 Msa0563650:intron 35.0%
!! TTTTTGTTTGGCCTATTAGG+GGG + chr4_1:64090634-64090653 None:intergenic 35.0%
!! GTTTTTGTTTGGCCTATTAG+GGG + chr4_1:64090635-64090654 None:intergenic 35.0%
!! CGTTTTTGTTTGGCCTATTA+GGG + chr4_1:64090636-64090655 None:intergenic 35.0%
!! TCGTTTTTGTTTGGCCTATT+AGG + chr4_1:64090637-64090656 None:intergenic 35.0%
ATTATAATGCAGAGAGGGAA+AGG - chr4_1:64090908-64090927 Msa0563650:CDS 35.0%
TTATAATGCAGAGAGGGAAA+GGG - chr4_1:64090909-64090928 Msa0563650:CDS 35.0%
AGGGATTTAACAATTCCCTA+TGG - chr4_1:64090949-64090968 Msa0563650:intron 35.0%
GATATACCTTCTCAAGGATT+AGG - chr4_1:64090998-64091017 Msa0563650:intron 35.0%
TATCATCCTAATCCTTGAGA+AGG + chr4_1:64091007-64091026 None:intergenic 35.0%
ATGAACATGCTATGGAAGAA+AGG + chr4_1:64091037-64091056 None:intergenic 35.0%
TCAAAACATGAACAGGAACT+GGG + chr4_1:64091396-64091415 None:intergenic 35.0%
ATCAAAACATGAACAGGAAC+TGG + chr4_1:64091397-64091416 None:intergenic 35.0%
!! GGGATTAGTCATCGTATAAA+TGG - chr4_1:64091546-64091565 Msa0563650:five_prime_UTR 35.0%
!! ACGAACCATGATTGATTTGT+AGG + chr4_1:64089697-64089716 None:intergenic 35.0%
GAATGCAAGAAATAGAAGAG+AGG + chr4_1:64089726-64089745 None:intergenic 35.0%
! CCTTTCTTAACAGTTGTTTC+TGG + chr4_1:64090186-64090205 None:intergenic 35.0%
CCAGAAACAACTGTTAAGAA+AGG - chr4_1:64090183-64090202 Msa0563650:intron 35.0%
AAAGGAAATGACTATCCTGT+TGG - chr4_1:64090201-64090220 Msa0563650:intron 35.0%
! TTCTTCGTCTTTGTTGTCAT+TGG - chr4_1:64090240-64090259 Msa0563650:intron 35.0%
TAAGGAGGACAACATTGAAA+GGG + chr4_1:64090280-64090299 None:intergenic 35.0%
ATAAGGAGGACAACATTGAA+AGG + chr4_1:64090281-64090300 None:intergenic 35.0%
CCCAATTCATCACAAGAATT+AGG + chr4_1:64090341-64090360 None:intergenic 35.0%
!!! GCTCAACCGATTTTAACTAT+TGG + chr4_1:64090426-64090445 None:intergenic 35.0%
!! GGTTCAATCGTTGTTTTGTT+TGG - chr4_1:64090481-64090500 Msa0563650:intron 35.0%
TCAAGCTGTTGAACTATTTG+AGG - chr4_1:64090505-64090524 Msa0563650:intron 35.0%
! TTGAACTATTTGAGGTCTTG+TGG - chr4_1:64090513-64090532 Msa0563650:intron 35.0%
AGGTCTTTACCCTGTAATAT+AGG - chr4_1:64090581-64090600 Msa0563650:intron 35.0%
!!! GTTTTGATTTACCCCCTAAT+AGG - chr4_1:64090620-64090639 Msa0563650:intron 35.0%
!! TTTTTGTTTGGCCTATTAGG+GGG + chr4_1:64090634-64090653 None:intergenic 35.0%
!! GTTTTTGTTTGGCCTATTAG+GGG + chr4_1:64090635-64090654 None:intergenic 35.0%
!! CGTTTTTGTTTGGCCTATTA+GGG + chr4_1:64090636-64090655 None:intergenic 35.0%
!! TCGTTTTTGTTTGGCCTATT+AGG + chr4_1:64090637-64090656 None:intergenic 35.0%
ATTATAATGCAGAGAGGGAA+AGG - chr4_1:64090908-64090927 Msa0563650:CDS 35.0%
TTATAATGCAGAGAGGGAAA+GGG - chr4_1:64090909-64090928 Msa0563650:CDS 35.0%
AGGGATTTAACAATTCCCTA+TGG - chr4_1:64090949-64090968 Msa0563650:intron 35.0%
GATATACCTTCTCAAGGATT+AGG - chr4_1:64090998-64091017 Msa0563650:intron 35.0%
TATCATCCTAATCCTTGAGA+AGG + chr4_1:64091007-64091026 None:intergenic 35.0%
ATGAACATGCTATGGAAGAA+AGG + chr4_1:64091037-64091056 None:intergenic 35.0%
TCAAAACATGAACAGGAACT+GGG + chr4_1:64091396-64091415 None:intergenic 35.0%
ATCAAAACATGAACAGGAAC+TGG + chr4_1:64091397-64091416 None:intergenic 35.0%
!! GGGATTAGTCATCGTATAAA+TGG - chr4_1:64091546-64091565 Msa0563650:five_prime_UTR 35.0%
! ACCACCGTCTTTCAATTTTC+CGG - chr4_1:64089637-64089656 Msa0563650:three_prime_UTR 40.0%
AACGCCGGAAAATTGAAAGA+CGG + chr4_1:64089644-64089663 None:intergenic 40.0%
!! TTTGTAGGATAATGGATCGC+CGG + chr4_1:64089682-64089701 None:intergenic 40.0%
ACGAAGAAACCAAGCAAAAC+GGG + chr4_1:64090228-64090247 None:intergenic 40.0%
GACGAAGAAACCAAGCAAAA+CGG + chr4_1:64090229-64090248 None:intergenic 40.0%
!! GGTAATGGGTAATGGTCAAA+GGG - chr4_1:64090930-64090949 Msa0563650:CDS 40.0%
CATAGCTCATGAACATGCTA+TGG + chr4_1:64091045-64091064 None:intergenic 40.0%
CAGCTCTCTTTCAGATAATC+AGG - chr4_1:64091259-64091278 Msa0563650:intron 40.0%
ATTGATGCTGTGTAATCGTG+AGG - chr4_1:64091525-64091544 Msa0563650:five_prime_UTR 40.0%
TTGATGCTGTGTAATCGTGA+GGG - chr4_1:64091526-64091545 Msa0563650:five_prime_UTR 40.0%
! ACCACCGTCTTTCAATTTTC+CGG - chr4_1:64089637-64089656 Msa0563650:three_prime_UTR 40.0%
AACGCCGGAAAATTGAAAGA+CGG + chr4_1:64089644-64089663 None:intergenic 40.0%
!! TTTGTAGGATAATGGATCGC+CGG + chr4_1:64089682-64089701 None:intergenic 40.0%
ACGAAGAAACCAAGCAAAAC+GGG + chr4_1:64090228-64090247 None:intergenic 40.0%
GACGAAGAAACCAAGCAAAA+CGG + chr4_1:64090229-64090248 None:intergenic 40.0%
!! GGTAATGGGTAATGGTCAAA+GGG - chr4_1:64090930-64090949 Msa0563650:CDS 40.0%
CATAGCTCATGAACATGCTA+TGG + chr4_1:64091045-64091064 None:intergenic 40.0%
CAGCTCTCTTTCAGATAATC+AGG - chr4_1:64091259-64091278 Msa0563650:intron 40.0%
ATTGATGCTGTGTAATCGTG+AGG - chr4_1:64091525-64091544 Msa0563650:five_prime_UTR 40.0%
TTGATGCTGTGTAATCGTGA+GGG - chr4_1:64091526-64091545 Msa0563650:five_prime_UTR 40.0%
TGAACGGTGATCGTTGTTAC+CGG + chr4_1:64089557-64089576 None:intergenic 45.0%
!! ACGGTGGTGATGTTCTTTTC+CGG + chr4_1:64089625-64089644 None:intergenic 45.0%
TGGAAAAACGAAGAGAACGC+CGG + chr4_1:64089659-64089678 None:intergenic 45.0%
!! CGTTCTCTTCGTTTTTCCAC+CGG - chr4_1:64089660-64089679 Msa0563650:three_prime_UTR 45.0%
TCTCAACCTTCCTAACTGCA+AGG + chr4_1:64090128-64090147 None:intergenic 45.0%
CGAAGAAACCAAGCAAAACG+GGG + chr4_1:64090227-64090246 None:intergenic 45.0%
!! AGGGAAAGGGTAATGGGTAA+TGG - chr4_1:64090922-64090941 Msa0563650:CDS 45.0%
!! GGGTAATGGGTAATGGTCAA+AGG - chr4_1:64090929-64090948 Msa0563650:CDS 45.0%
ATAATCAGGACAGCAACGAG+TGG - chr4_1:64091273-64091292 Msa0563650:intron 45.0%
!! GGCATAATGGAGCTTTGAGT+TGG - chr4_1:64091302-64091321 Msa0563650:intron 45.0%
TGAACGGTGATCGTTGTTAC+CGG + chr4_1:64089557-64089576 None:intergenic 45.0%
!! ACGGTGGTGATGTTCTTTTC+CGG + chr4_1:64089625-64089644 None:intergenic 45.0%
TGGAAAAACGAAGAGAACGC+CGG + chr4_1:64089659-64089678 None:intergenic 45.0%
!! CGTTCTCTTCGTTTTTCCAC+CGG - chr4_1:64089660-64089679 Msa0563650:three_prime_UTR 45.0%
TCTCAACCTTCCTAACTGCA+AGG + chr4_1:64090128-64090147 None:intergenic 45.0%
CGAAGAAACCAAGCAAAACG+GGG + chr4_1:64090227-64090246 None:intergenic 45.0%
!! AGGGAAAGGGTAATGGGTAA+TGG - chr4_1:64090922-64090941 Msa0563650:CDS 45.0%
!! GGGTAATGGGTAATGGTCAA+AGG - chr4_1:64090929-64090948 Msa0563650:CDS 45.0%
ATAATCAGGACAGCAACGAG+TGG - chr4_1:64091273-64091292 Msa0563650:intron 45.0%
!! GGCATAATGGAGCTTTGAGT+TGG - chr4_1:64091302-64091321 Msa0563650:intron 45.0%
!! AAATAAATAAATAAAAAAGT+TGG - chr4_1:64089521-64089540 Msa0563650:three_prime_UTR 5.0%
!! AAATAAATAAATAAAAAAGT+TGG - chr4_1:64089521-64089540 Msa0563650:three_prime_UTR 5.0%
GCCGGAAAATTGAAAGACGG+TGG + chr4_1:64089641-64089660 None:intergenic 50.0%
! CATCAAAGCGCCTTGCAGTT+AGG - chr4_1:64090115-64090134 Msa0563650:intron 50.0%
! AAAGCGCCTTGCAGTTAGGA+AGG - chr4_1:64090119-64090138 Msa0563650:intron 50.0%
TGCAGAGAGGGAAAGGGTAA+TGG - chr4_1:64090915-64090934 Msa0563650:CDS 50.0%
GCAGAGAGGGAAAGGGTAAT+GGG - chr4_1:64090916-64090935 Msa0563650:CDS 50.0%
! CACGGAGCACCAATTCCATA+GGG + chr4_1:64090967-64090986 None:intergenic 50.0%
! TCACGGAGCACCAATTCCAT+AGG + chr4_1:64090968-64090987 None:intergenic 50.0%
GCCGGAAAATTGAAAGACGG+TGG + chr4_1:64089641-64089660 None:intergenic 50.0%
! CATCAAAGCGCCTTGCAGTT+AGG - chr4_1:64090115-64090134 Msa0563650:intron 50.0%
! AAAGCGCCTTGCAGTTAGGA+AGG - chr4_1:64090119-64090138 Msa0563650:intron 50.0%
TGCAGAGAGGGAAAGGGTAA+TGG - chr4_1:64090915-64090934 Msa0563650:CDS 50.0%
GCAGAGAGGGAAAGGGTAAT+GGG - chr4_1:64090916-64090935 Msa0563650:CDS 50.0%
! CACGGAGCACCAATTCCATA+GGG + chr4_1:64090967-64090986 None:intergenic 50.0%
! TCACGGAGCACCAATTCCAT+AGG + chr4_1:64090968-64090987 None:intergenic 50.0%
ACCTCGGAGAGATTCACTGC+CGG - chr4_1:64089603-64089622 Msa0563650:three_prime_UTR 55.0%
!! GTAGGATAATGGATCGCCGG+TGG + chr4_1:64089679-64089698 None:intergenic 55.0%
ATCAGGACAGCAACGAGTGG+AGG - chr4_1:64091276-64091295 Msa0563650:intron 55.0%
! CGAGTGGAGGCATGGCATAA+TGG - chr4_1:64091289-64091308 Msa0563650:intron 55.0%
ACCTCGGAGAGATTCACTGC+CGG - chr4_1:64089603-64089622 Msa0563650:three_prime_UTR 55.0%
!! GTAGGATAATGGATCGCCGG+TGG + chr4_1:64089679-64089698 None:intergenic 55.0%
ATCAGGACAGCAACGAGTGG+AGG - chr4_1:64091276-64091295 Msa0563650:intron 55.0%
! CGAGTGGAGGCATGGCATAA+TGG - chr4_1:64091289-64091308 Msa0563650:intron 55.0%
TCCGGCAGTGAATCTCTCCG+AGG + chr4_1:64089607-64089626 None:intergenic 60.0%
CCAAGCAAAACGGGGCCAAC+AGG + chr4_1:64090219-64090238 None:intergenic 60.0%
! CCTGTTGGCCCCGTTTTGCT+TGG - chr4_1:64090216-64090235 Msa0563650:intron 60.0%
GACAGCAACGAGTGGAGGCA+TGG - chr4_1:64091281-64091300 Msa0563650:intron 60.0%
TCCGGCAGTGAATCTCTCCG+AGG + chr4_1:64089607-64089626 None:intergenic 60.0%
CCAAGCAAAACGGGGCCAAC+AGG + chr4_1:64090219-64090238 None:intergenic 60.0%
! CCTGTTGGCCCCGTTTTGCT+TGG - chr4_1:64090216-64090235 Msa0563650:intron 60.0%
GACAGCAACGAGTGGAGGCA+TGG - chr4_1:64091281-64091300 Msa0563650:intron 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 64089482 64091579 64089482 ID=Msa0563650;Name=Msa0563650
chr4_1 mRNA 64089482 64091579 64089482 ID=Msa0563650-mRNA-1;Parent=Msa0563650;Name=Msa0563650-mRNA-1;_AED=0.07;_eAED=0.07;_QI=226|1|1|1|1|1|3|271|68
chr4_1 exon 64089482 64089799 64089482 ID=Msa0563650-mRNA-1:exon:20460;Parent=Msa0563650-mRNA-1
chr4_1 exon 64090794 64090950 64090794 ID=Msa0563650-mRNA-1:exon:20459;Parent=Msa0563650-mRNA-1
chr4_1 exon 64091351 64091579 64091351 ID=Msa0563650-mRNA-1:exon:20458;Parent=Msa0563650-mRNA-1
chr4_1 five_prime_UTR 64091354 64091579 64091354 ID=Msa0563650-mRNA-1:five_prime_utr;Parent=Msa0563650-mRNA-1
chr4_1 CDS 64091351 64091353 64091351 ID=Msa0563650-mRNA-1:cds;Parent=Msa0563650-mRNA-1
chr4_1 CDS 64090794 64090950 64090794 ID=Msa0563650-mRNA-1:cds;Parent=Msa0563650-mRNA-1
chr4_1 CDS 64089753 64089799 64089753 ID=Msa0563650-mRNA-1:cds;Parent=Msa0563650-mRNA-1
chr4_1 three_prime_UTR 64089482 64089752 64089482 ID=Msa0563650-mRNA-1:three_prime_utr;Parent=Msa0563650-mRNA-1
Gene Sequence

>Msa0563650

ATGACTACATCAAAGCGCCTTGCAGTTAGGAAGGTTGAGAAGTTCGAGAAAAATATCACAAAAAGAGGAAGTGTGCCAGAAACAACTGTTAAGAAAGGAAATGACTATCCTGTTGGCCCCGTTTTGCTTGGTTTCTTCGTCTTTGTTGTCATTGGATCATCTCTCTTTCAGATAATCAGGACAGCAACGAGTGGAGGCATGGCATAA
Protein sequence

>Msa0563650

MTTSKRLAVRKVEKFEKNITKRGSVPETTVKKGNDYPVGPVLLGFFVFVVIGSSLFQIIRTATSGGMA*