AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0652700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0652700 MtrunA17_Chr4g0046401 91.086 718 35 1 1 689 1 718 0.0 1332
Msa0652700 MtrunA17_Chr1g0186381 60.139 720 254 5 5 694 6 722 0.0 904
Msa0652700 MtrunA17_Chr2g0307191 43.902 697 337 11 20 683 15 690 0.0 594
Msa0652700 MtrunA17_Chr3g0095791 42.529 696 347 9 20 683 15 689 0.0 587
Msa0652700 MtrunA17_Chr2g0298581 41.404 698 355 8 20 684 15 691 0.0 582
Msa0652700 MtrunA17_Chr3g0077511 41.117 698 357 7 20 683 15 692 0.0 575
Msa0652700 MtrunA17_Chr3g0102541 35.766 685 384 10 59 698 52 725 1.28e-141 432
Msa0652700 MtrunA17_Chr3g0102531 35.389 681 388 9 59 698 52 721 7.31e-141 429
Msa0652700 MtrunA17_Chr3g0102551 35.302 728 402 16 21 698 21 729 7.41e-139 425
Msa0652700 MtrunA17_Chr4g0073711 31.365 762 405 16 24 683 251 996 8.72e-116 372
Msa0652700 MtrunA17_Chr2g0284211 31.532 777 411 16 15 683 172 935 4.56e-115 369
Msa0652700 MtrunA17_Chr8g0379241 31.552 786 415 19 6 683 177 947 4.22e-114 367
Msa0652700 MtrunA17_Chr8g0364641 31.447 760 400 18 19 683 206 939 1.26e-113 365
Msa0652700 MtrunA17_Chr1g0183381 30.880 761 410 18 24 686 123 865 8.31e-110 353
Msa0652700 MtrunA17_Chr2g0322361 31.721 703 407 18 40 684 37 724 1.64e-107 343
Msa0652700 MtrunA17_Chr2g0322371 32.507 686 389 18 57 684 45 714 3.74e-106 339
Msa0652700 MtrunA17_Chr2g0322351 32.810 637 367 12 57 645 47 670 6.37e-101 325
Msa0652700 MtrunA17_Chr4g0025201 30.312 673 408 15 57 677 47 710 1.10e-98 320
Msa0652700 MtrunA17_Chr5g0410641 28.469 836 421 20 14 683 258 1082 6.02e-92 309
Msa0652700 MtrunA17_Chr4g0039801 28.485 825 415 18 24 683 289 1103 5.26e-91 307
Msa0652700 MtrunA17_Chr7g0269741 27.976 840 422 19 15 683 304 1131 3.16e-89 302
Msa0652700 MtrunA17_Chr1g0165411 27.785 817 420 19 24 683 261 1064 4.48e-88 298
Msa0652700 MtrunA17_Chr1g0165421 27.885 832 414 21 24 683 275 1092 5.86e-87 296
Msa0652700 MtrunA17_Chr2g0297351 26.723 827 427 22 24 686 175 986 3.27e-85 289
Msa0652700 MtrunA17_Chr8g0383701 36.387 382 210 7 24 382 289 660 5.86e-73 256
Msa0652700 MtrunA17_Chr8g0383701 31.778 343 190 8 379 683 718 1054 6.36e-43 167
Msa0652700 MtrunA17_Chr7g0267731 36.772 378 212 8 24 381 183 553 3.02e-72 253
Msa0652700 MtrunA17_Chr7g0267731 29.966 297 168 5 421 683 654 944 2.91e-35 143
Msa0652700 MtrunA17_Chr1g0201731 36.675 379 213 8 24 382 291 662 3.04e-71 251
Msa0652700 MtrunA17_Chr1g0201731 31.902 326 184 6 390 682 734 1054 3.69e-39 155
Msa0652700 MtrunA17_Chr3g0088821 38.123 341 190 4 59 379 300 639 9.95e-71 249
Msa0652700 MtrunA17_Chr3g0088821 29.358 327 194 5 390 683 714 1036 4.72e-38 152
Msa0652700 MtrunA17_Chr2g0296991 36.412 379 208 9 24 379 222 590 3.31e-70 248
Msa0652700 MtrunA17_Chr2g0296991 30.530 321 185 5 396 683 687 1002 8.27e-40 157
Msa0652700 MtrunA17_Chr8g0352231 37.609 343 193 4 59 381 300 641 1.13e-68 244
Msa0652700 MtrunA17_Chr8g0352231 30.218 321 187 5 396 683 720 1036 3.59e-39 155
Msa0652700 MtrunA17_Chr3g0137951 36.436 376 212 7 24 379 281 649 2.50e-67 240
Msa0652700 MtrunA17_Chr3g0137951 29.755 326 190 6 392 683 724 1044 3.88e-38 152
Msa0652700 MtrunA17_Chr7g0274751 33.137 510 241 14 59 470 141 648 3.51e-67 236
Msa0652700 MtrunA17_Chr3g0078671 35.356 379 218 7 24 382 238 609 3.60e-67 239
Msa0652700 MtrunA17_Chr3g0078671 28.116 345 208 8 376 683 666 1007 1.13e-38 154
Msa0652700 MtrunA17_Chr2g0322341 37.097 310 175 6 53 349 45 347 5.88e-64 216
Msa0652700 MtrunA17_Chr2g0307241 47.312 186 89 2 20 205 15 191 3.15e-58 196
Msa0652700 MtrunA17_Chr2g0322331 25.000 328 211 5 391 684 29 355 6.58e-21 95.5
Msa0652700 MtrunA17_Chr2g0307231 46.078 102 50 2 333 430 2 102 5.14e-18 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0652700 AT4G24000.1 37.083 720 382 15 10 688 11 700 1.28e-154 465
Msa0652700 AT4G24010.1 36.276 725 396 13 10 692 14 714 3.09e-152 460
Msa0652700 AT4G23990.1 36.791 723 395 11 6 688 29 729 8.73e-150 454
Msa0652700 AT1G55850.1 33.921 681 386 10 57 698 64 719 1.33e-129 400
Msa0652700 AT5G17420.1 31.868 728 383 13 59 683 267 984 8.87e-117 375
Msa0652700 AT5G64740.1 31.202 782 401 18 24 683 276 1042 2.30e-113 367
Msa0652700 AT5G09870.1 30.680 779 406 19 24 683 264 1027 8.48e-110 357
Msa0652700 AT2G25540.1 30.288 799 426 19 3 683 233 1018 2.50e-109 356
Msa0652700 AT4G24000.2 36.415 530 277 10 196 688 2 508 4.64e-109 341
Msa0652700 AT4G32410.1 30.670 776 410 18 24 683 275 1038 9.02e-109 355
Msa0652700 AT4G18780.1 31.443 776 398 17 24 683 184 941 1.37e-108 352
Msa0652700 AT4G23990.2 36.415 530 284 8 196 688 2 515 5.19e-104 328
Msa0652700 AT2G32540.1 29.630 702 398 17 59 684 51 732 6.68e-104 334
Msa0652700 AT2G32530.1 31.792 692 369 24 34 646 30 697 1.76e-103 333
Msa0652700 AT4G15320.3 30.683 717 398 20 37 677 36 729 7.45e-102 329
Msa0652700 AT4G15320.5 30.683 717 398 20 37 677 36 729 9.91e-102 328
Msa0652700 AT4G15320.4 30.737 706 390 20 59 677 51 744 5.51e-100 324
Msa0652700 AT2G32620.1 30.226 708 388 22 59 684 51 734 9.43e-98 318
Msa0652700 AT4G15290.1 31.259 659 367 19 59 647 51 693 1.29e-95 312
Msa0652700 AT2G32610.1 30.827 665 354 20 59 642 51 690 9.03e-94 307
Msa0652700 AT2G32620.2 35.388 503 267 14 59 518 51 538 1.05e-92 298
Msa0652700 AT2G32620.3 34.087 531 275 15 59 529 51 566 4.74e-92 299
Msa0652700 AT4G38190.1 28.218 808 420 18 24 683 272 1067 2.51e-91 308
Msa0652700 AT1G32180.1 27.929 845 419 22 15 683 113 943 1.82e-89 301
Msa0652700 AT1G32180.2 27.929 845 419 22 15 683 113 943 1.82e-89 301
Msa0652700 AT1G02730.1 27.896 846 408 22 24 686 318 1144 7.47e-88 299
Msa0652700 AT2G33100.2 27.108 830 422 23 24 683 183 999 1.00e-87 297
Msa0652700 AT2G33100.1 27.108 830 422 23 24 683 183 999 1.00e-87 297
Msa0652700 AT4G15320.2 30.539 668 326 20 37 602 36 667 7.94e-87 290
Msa0652700 AT5G16910.1 27.683 820 423 20 24 683 297 1106 3.59e-86 294
Msa0652700 AT2G32620.4 29.607 662 361 21 104 684 1 638 8.40e-86 284
Msa0652700 AT3G03050.1 26.488 823 432 17 24 683 294 1106 1.13e-84 290
Msa0652700 AT4G15320.1 29.197 685 319 21 59 602 51 710 7.22e-80 271
Msa0652700 AT4G39350.1 36.649 382 209 7 24 382 277 648 9.72e-71 250
Msa0652700 AT4G39350.1 31.498 327 186 6 390 683 720 1041 3.82e-36 147
Msa0652700 AT2G21770.1 36.111 396 219 8 8 382 271 653 1.72e-70 249
Msa0652700 AT2G21770.1 31.157 337 194 6 380 683 714 1045 1.36e-40 160
Msa0652700 AT5G05170.1 36.436 376 212 5 24 379 259 627 4.21e-70 248
Msa0652700 AT5G05170.1 29.358 327 194 5 390 683 701 1023 5.48e-38 152
Msa0652700 AT5G44030.2 36.148 379 209 9 24 379 244 612 4.77e-69 245
Msa0652700 AT5G44030.2 32.298 322 180 7 396 683 719 1036 9.45e-40 158
Msa0652700 AT5G44030.1 36.148 379 209 9 24 379 215 583 5.78e-69 244
Msa0652700 AT5G44030.1 32.298 322 180 7 396 683 690 1007 1.22e-39 157
Msa0652700 AT5G44030.3 39.016 305 166 6 91 379 4 304 8.06e-65 229
Msa0652700 AT5G44030.3 32.298 322 180 7 396 683 411 728 4.39e-40 158

Find 177 sgRNAs with CRISPR-Local

Find 251 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTAAAGTGGGTTCCTTAAT+TGG 0.141463 4_3:+60405022 None:intergenic
CTCTCCTTCATTTGGTTCCT+TGG 0.172567 4_3:-60405148 Msa0652700:CDS
CTCTTTATTGTTTGCCCCTT+TGG 0.176106 4_3:-60403086 Msa0652700:CDS
GGAAGTCGTATTTATCATTT+TGG 0.178591 4_3:+60402649 None:intergenic
CTGCAACTTCGAGAATATTT+TGG 0.180704 4_3:-60403591 Msa0652700:CDS
TAGGCTTCTTGTTCTATTAC+AGG 0.204037 4_3:-60405252 Msa0652700:CDS
TCAAGAGATGGTATTACTTT+TGG 0.207697 4_3:-60404585 Msa0652700:CDS
GATATGAAACTGGCACTAAA+TGG 0.224428 4_3:-60403464 Msa0652700:CDS
ATGGCATATCGAGCTGAATT+TGG 0.256825 4_3:+60404039 None:intergenic
CTGGAGGAACACTACATAAA+TGG 0.257412 4_3:-60402818 Msa0652700:CDS
TGATGATAAGCAACAACTTT+TGG 0.260318 4_3:+60405308 None:intergenic
CCTCAGTTCTGTCTACTAAA+TGG 0.263774 4_3:-60403048 Msa0652700:CDS
TCATCCTCTGGCAATCTCTC+TGG 0.273425 4_3:+60405085 None:intergenic
TTACTAGAAGTCTTCTTAAC+TGG 0.287101 4_3:-60402837 Msa0652700:CDS
TGGAGTTTATAGAGTGATAT+TGG 0.310644 4_3:-60402559 Msa0652700:CDS
TTCTCCCTACATCCTAGTTC+TGG 0.315742 4_3:-60404085 Msa0652700:CDS
TCATCCTCTGCTTTATTAGT+AGG 0.327364 4_3:+60402690 None:intergenic
GCAAGGGATGGACGGGCTTA+AGG 0.330461 4_3:-60403818 Msa0652700:CDS
AAATCATTCTCTCCTTCATT+TGG 0.335597 4_3:-60405156 Msa0652700:CDS
GGCAGAGAAGCAAAAGATTA+AGG 0.344678 4_3:-60404759 Msa0652700:intron
TTGCAATCTATATGGTTGTT+TGG 0.355256 4_3:-60402757 Msa0652700:CDS
TCAACTACTTGCAATCTATA+TGG 0.355613 4_3:-60402765 Msa0652700:CDS
GGATCAATGAGCAGAGAATC+TGG 0.363474 4_3:-60402797 Msa0652700:CDS
ATGCACTGCAGATCCAATTA+AGG 0.368192 4_3:-60405035 Msa0652700:CDS
GACAAGGAGGATGATATCTT+TGG 0.372812 4_3:+60404000 None:intergenic
CAAAGCAGAAAAGTAGGCCT+TGG 0.385342 4_3:+60404823 None:intergenic
GCTCGAAGAACCTCACTTGT+TGG 0.395209 4_3:-60403496 Msa0652700:CDS
ATATGAAACTGGCACTAAAT+GGG 0.398753 4_3:-60403463 Msa0652700:CDS
ATCACCAGCCCTTCAATGAT+AGG 0.399749 4_3:+60402471 None:intergenic
GAACTGCAAGGTTATCATCT+AGG 0.402438 4_3:-60405380 Msa0652700:five_prime_UTR
CTTGGGAGTTTGCAAGGTGG+TGG 0.404254 4_3:-60404886 Msa0652700:CDS
AATGTGCACCATTATATTAG+AGG 0.404986 4_3:-60401951 None:intergenic
AGTTGTTGCTTATCATCAAT+AGG 0.407981 4_3:-60405303 Msa0652700:CDS
GGGCAACCTGTATGAACATT+TGG 0.409650 4_3:+60402523 None:intergenic
CCAAGGAACTGTGGCCTAGA+TGG 0.410757 4_3:+60403267 None:intergenic
CAAGGGATGGACGGGCTTAA+GGG 0.413602 4_3:-60403817 Msa0652700:CDS
GCATTGTAACGGATGGAATT+CGG 0.422318 4_3:-60403301 Msa0652700:CDS
GCTTCTTGTAGATATGAAAC+TGG 0.425990 4_3:-60403474 Msa0652700:CDS
CATGTGTATCTTTCAGATGA+TGG 0.428039 4_3:-60404944 Msa0652700:CDS
CTGTGTTACCTTAGGATACA+AGG 0.430250 4_3:+60403020 None:intergenic
GATGCTTTACTGAAGAAAGT+TGG 0.432109 4_3:-60402735 Msa0652700:CDS
GGTTATCATCTAGGGAGAAA+TGG 0.433802 4_3:-60405371 Msa0652700:exon
AAAGCAGAAAAGTAGGCCTT+GGG 0.434065 4_3:+60404824 None:intergenic
GAGTGATATTGGTGGGTGAT+TGG 0.434933 4_3:-60402548 Msa0652700:CDS
AGGAAAGGCCTCTAATATAA+TGG 0.441337 4_3:+60401943 None:intergenic
TGAGGGCTCCAGCTTTGAAA+TGG 0.444970 4_3:+60404240 None:intergenic
CATCCTCTGGCAATCTCTCT+GGG 0.449447 4_3:+60405086 None:intergenic
TCATTGAAGGGCTGGTGATA+AGG 0.449469 4_3:-60402467 Msa0652700:CDS
TACTAATCCAAGGCACTAGA+TGG 0.455870 4_3:-60403218 Msa0652700:CDS
TCCTTGGCCAAGCTTATCGC+TGG 0.456621 4_3:-60405132 Msa0652700:CDS
TCGAGCAATGTATTCCGTCC+TGG 0.457971 4_3:+60403513 None:intergenic
ATAAACTACCCTATCATTGA+AGG 0.469163 4_3:-60402480 Msa0652700:CDS
CTCCTTCATTCCATGGCTCT+AGG 0.469797 4_3:-60405271 Msa0652700:CDS
TGGGAAGACATAACGTGAAA+CGG 0.477193 4_3:+60405105 None:intergenic
AGGCTTCTTGTTCTATTACA+GGG 0.479759 4_3:-60405251 Msa0652700:CDS
CTACCCTATCATTGAAGGGC+TGG 0.482472 4_3:-60402475 Msa0652700:CDS
CAATTTGCAGAGAAAGTATG+AGG 0.482474 4_3:-60404652 Msa0652700:intron
AGTCCCCACCATTTCAAAGC+TGG 0.483676 4_3:-60404248 Msa0652700:CDS
GGGAAGACATAACGTGAAAC+GGG 0.483928 4_3:+60405106 None:intergenic
GGCTCCAGCTTTGAAATGGT+GGG 0.486191 4_3:+60404244 None:intergenic
AGTGATATTGGTGGGTGATT+GGG 0.486626 4_3:-60402547 Msa0652700:CDS
GCAGGGAGCTTGTCATCCTC+TGG 0.486915 4_3:+60405073 None:intergenic
AGTGTTCATCACTTCTAAAG+TGG 0.487025 4_3:+60405009 None:intergenic
CCATCTAGGCCACAGTTCCT+TGG 0.487196 4_3:-60403267 Msa0652700:CDS
ACACATGCTCCTTCATTCCA+TGG 0.492247 4_3:-60405278 Msa0652700:CDS
CGAGCAATGTATTCCGTCCT+GGG 0.494664 4_3:+60403514 None:intergenic
GTATGGTGTGAGGAAGGCTT+GGG 0.495077 4_3:-60404903 Msa0652700:CDS
TATTCTGTACCTTCTGCAGA+AGG 0.499017 4_3:+60404859 None:intergenic
TTGTAATGATAAAACTTGCA+AGG 0.499402 4_3:+60402502 None:intergenic
GAAGGTACAGAATAAAGAAC+AGG 0.500742 4_3:-60404850 Msa0652700:CDS
TGTATGGTGTGAGGAAGGCT+TGG 0.500918 4_3:-60404904 Msa0652700:CDS
CAGAGTACCATCTAGTGCCT+TGG 0.501479 4_3:+60403211 None:intergenic
CAAGGAGCCATGGTAACAAA+TGG 0.502268 4_3:+60405191 None:intergenic
TTGGGACCAAATGTTCATAC+AGG 0.504326 4_3:-60402529 Msa0652700:CDS
CAAAGGGGCAAACAATAAAG+AGG 0.504519 4_3:+60403087 None:intergenic
AGCCTAGAGCCATGGAATGA+AGG 0.506111 4_3:+60405269 None:intergenic
GGATTTATCTTGCATTGTAA+CGG 0.506211 4_3:-60403312 Msa0652700:CDS
GGTGGCTTCCCTTCTGCAGA+AGG 0.510610 4_3:-60404868 Msa0652700:CDS
AACTGCAAGGTTATCATCTA+GGG 0.511603 4_3:-60405379 Msa0652700:five_prime_UTR
TTATCTTGCATTGTAACGGA+TGG 0.511997 4_3:-60403308 Msa0652700:CDS
TGCCAGGAGTAGTGTTTACA+TGG 0.512757 4_3:-60404780 Msa0652700:CDS
GATGGTGGCAAAACACCAAA+GGG 0.515466 4_3:+60403071 None:intergenic
TGCCATGTAAACACTACTCC+TGG 0.515527 4_3:+60404778 None:intergenic
GTAGTCGAAGATTTCCTGAC+TGG 0.518872 4_3:-60403333 Msa0652700:CDS
ACAATCCAGAACTAGGATGT+AGG 0.519220 4_3:+60404080 None:intergenic
CTTAAGGGACCTGTATTGTC+AGG 0.520784 4_3:-60403802 Msa0652700:CDS
TACAATGGCAAGGGATGGAC+GGG 0.522235 4_3:-60403825 Msa0652700:CDS
AATGCAAGATAAATCCAGTC+AGG 0.527019 4_3:+60403319 None:intergenic
TGGAATACCCTTGTATCCTA+AGG 0.531703 4_3:-60403028 Msa0652700:intron
TATTGAGAGGAAGAGTCCCT+TGG 0.531960 4_3:+60405341 None:intergenic
AAGCATGTACCTGACAATAC+AGG 0.534743 4_3:+60403793 None:intergenic
GAACAAGAAGCCTAGAGCCA+TGG 0.535046 4_3:+60405261 None:intergenic
TTGAACAACTTCAGCTACAA+TGG 0.536811 4_3:-60403840 Msa0652700:intron
TGTAATGATAAAACTTGCAA+GGG 0.538161 4_3:+60402503 None:intergenic
ATTGAGAGGAAGAGTCCCTT+GGG 0.539211 4_3:+60405342 None:intergenic
GGGCTCCAGCTTTGAAATGG+TGG 0.541590 4_3:+60404243 None:intergenic
CAAGGCACTAGATGGTACTC+TGG 0.543521 4_3:-60403210 Msa0652700:intron
ACTCTCCAAAGCAGAAAAGT+AGG 0.544277 4_3:+60404817 None:intergenic
CAATCCAGAACTAGGATGTA+GGG 0.544829 4_3:+60404081 None:intergenic
ATGAAAAGGGTATCATTGTA+CGG 0.545021 4_3:-60403766 Msa0652700:CDS
CTCACACCATACAGAGTCAT+GGG 0.545146 4_3:+60404914 None:intergenic
ACATGTCACAATCCAGAACT+AGG 0.545632 4_3:+60404073 None:intergenic
GGAAGGCTTGGGAGTTTGCA+AGG 0.546262 4_3:-60404892 Msa0652700:CDS
TACGGAAATCAAGCGAATGA+AGG 0.548147 4_3:-60403748 Msa0652700:intron
CGGGACGCCAGCGATAAGCT+TGG 0.548345 4_3:+60405125 None:intergenic
ATGTGTATCTTTCAGATGAT+GGG 0.551013 4_3:-60404943 Msa0652700:CDS
CTACAATGGCAAGGGATGGA+CGG 0.551068 4_3:-60403826 Msa0652700:CDS
AAATATGTTGATTGTGAGGA+GGG 0.553265 4_3:+60402593 None:intergenic
TCACCAGCCCTTCAATGATA+GGG 0.553988 4_3:+60402472 None:intergenic
CTCGAAACATAGACAAGTTG+TGG 0.554608 4_3:+60404287 None:intergenic
AGGATGATATCTTTGGATCA+AGG 0.554905 4_3:+60404007 None:intergenic
AATATTCTCGAAGTTGCAGC+AGG 0.557617 4_3:+60403595 None:intergenic
CATATGAACACATCAACAGC+AGG 0.558578 4_3:+60405055 None:intergenic
ATTGTGAGGAGGGCAAGCAT+TGG 0.559251 4_3:+60402603 None:intergenic
CTAGAAGTCTTCTTAACTGG+AGG 0.559844 4_3:-60402834 Msa0652700:CDS
GGATGGTGGCAAAACACCAA+AGG 0.560347 4_3:+60403070 None:intergenic
ATGGCCGTACAAGGACATTG+AGG 0.561723 4_3:+60404222 None:intergenic
TTGAATGATGTACTAATCCA+AGG 0.561797 4_3:-60403228 Msa0652700:CDS
GACTCTGTATGGTGTGAGGA+AGG 0.567745 4_3:-60404909 Msa0652700:CDS
TGGCCGTACAAGGACATTGA+GGG 0.568429 4_3:+60404223 None:intergenic
AGATCATTCATCAGTCATTG+AGG 0.572393 4_3:-60404562 Msa0652700:intron
CTAATGTTGTGAAATTTCTG+AGG 0.573072 4_3:+60403964 None:intergenic
TTGGCCAAGGAACCAAATGA+AGG 0.575063 4_3:+60405144 None:intergenic
CCTCACACCATACAGAGTCA+TGG 0.580155 4_3:+60404913 None:intergenic
AGTAATACCATCTCTTGAGA+AGG 0.580252 4_3:+60404590 None:intergenic
AGAAGATATCATTGATGATG+TGG 0.580750 4_3:-60404324 Msa0652700:CDS
GACAAAATATGTTGATTGTG+AGG 0.583493 4_3:+60402589 None:intergenic
GATGAGGCCTTCTCAAGAGA+TGG 0.584597 4_3:-60404597 Msa0652700:CDS
GTGTTCATCACTTCTAAAGT+GGG 0.585414 4_3:+60405010 None:intergenic
GCTCCAGCTTTGAAATGGTG+GGG 0.590422 4_3:+60404245 None:intergenic
GGGGAAGCCATTTGTTACCA+TGG 0.592373 4_3:-60405198 Msa0652700:CDS
AGAAACATCAAGGAACTGCA+AGG 0.593626 4_3:-60405392 None:intergenic
CAACTTCAGCTACAATGGCA+AGG 0.607668 4_3:-60403835 Msa0652700:intron
TGGACATGGCAGGTATTGAG+AGG 0.609066 4_3:+60405328 None:intergenic
GCCAGCGATAAGCTTGGCCA+AGG 0.610444 4_3:+60405131 None:intergenic
CAAGGGACGCATTTCACAGT+CGG 0.610991 4_3:-60402439 Msa0652700:intron
AAAGGGGCAAACAATAAAGA+GGG 0.612126 4_3:+60403088 None:intergenic
TAGACAGAACTGAGGGATGG+TGG 0.613347 4_3:+60403056 None:intergenic
CATTTAGTAGACAGAACTGA+GGG 0.613491 4_3:+60403049 None:intergenic
TGAAGGGCTGGTGATAAGGA+AGG 0.613741 4_3:-60402463 Msa0652700:CDS
TACACATGAATCTTCTGTTG+AGG 0.617007 4_3:+60404959 None:intergenic
TCAGCTACAATGGCAAGGGA+TGG 0.617186 4_3:-60403830 Msa0652700:intron
AAAATATGTTGATTGTGAGG+AGG 0.617494 4_3:+60402592 None:intergenic
TAAACTACCCTATCATTGAA+GGG 0.618147 4_3:-60402479 Msa0652700:CDS
CTTACCTACTAATAAAGCAG+AGG 0.620480 4_3:-60402694 Msa0652700:CDS
TATGAAACTGGCACTAAATG+GGG 0.620691 4_3:-60403462 Msa0652700:CDS
ATTCTGTACCTTCTGCAGAA+GGG 0.622468 4_3:+60404860 None:intergenic
AGAGACATTCAGAAAAGATG+AGG 0.623555 4_3:-60404613 Msa0652700:CDS
TGTGTTACCTTAGGATACAA+GGG 0.624805 4_3:+60403021 None:intergenic
TTTCCAAGACCAAGAAAACA+GGG 0.624831 4_3:-60405218 Msa0652700:CDS
AACTTCAGCTACAATGGCAA+GGG 0.624923 4_3:-60403834 Msa0652700:intron
GTTTATAGAGTGATATTGGT+GGG 0.628743 4_3:-60402555 Msa0652700:CDS
AGTTTATAGAGTGATATTGG+TGG 0.632616 4_3:-60402556 Msa0652700:CDS
GGAGAAATGGAGGTAACCCA+AGG 0.634770 4_3:-60405358 Msa0652700:exon
AATAAAGAACAGGTGCCCCA+AGG 0.644833 4_3:-60404840 Msa0652700:CDS
TTGAGGATAATCTAGTGCCA+TGG 0.650068 4_3:+60404976 None:intergenic
TGGATCAAGGTGAAAACACA+TGG 0.655681 4_3:+60404020 None:intergenic
CTACAAGAAGCCAACAAGTG+AGG 0.656944 4_3:+60403486 None:intergenic
GCTGGTGATAAGGAAGGACA+AGG 0.659987 4_3:-60402457 Msa0652700:CDS
AATTTGTCGTAGCTGTACCA+AGG 0.662023 4_3:+60403250 None:intergenic
TAGTAGACAGAACTGAGGGA+TGG 0.662046 4_3:+60403053 None:intergenic
GAGAAATGGAGGTAACCCAA+GGG 0.667623 4_3:-60405357 Msa0652700:exon
AGGAAATTGAACAAAAGACA+AGG 0.668900 4_3:+60403984 None:intergenic
TATCATCTAGGGAGAAATGG+AGG 0.679190 4_3:-60405368 Msa0652700:exon
CCATTTAGTAGACAGAACTG+AGG 0.684834 4_3:+60403048 None:intergenic
CCATGACTCTGTATGGTGTG+AGG 0.687665 4_3:-60404913 Msa0652700:CDS
GTAGCTGTACCAAGGAACTG+TGG 0.688487 4_3:+60403258 None:intergenic
CTTCCCAGAGAGATTGCCAG+AGG 0.691580 4_3:-60405089 Msa0652700:CDS
TCACACCATACAGAGTCATG+GGG 0.693106 4_3:+60404915 None:intergenic
AGGCTTGGGAGTTTGCAAGG+TGG 0.698000 4_3:-60404889 Msa0652700:CDS
TTCCAAGACCAAGAAAACAG+GGG 0.702380 4_3:-60405217 Msa0652700:CDS
CTGGTGATAAGGAAGGACAA+GGG 0.707930 4_3:-60402456 Msa0652700:CDS
AAGCAGAAAAGTAGGCCTTG+GGG 0.718114 4_3:+60404825 None:intergenic
AAATTGAACAAAAGACAAGG+AGG 0.720972 4_3:+60403987 None:intergenic
ATATGAACACATCAACAGCA+GGG 0.739860 4_3:+60405056 None:intergenic
ATGGTGGCAAAACACCAAAG+GGG 0.740329 4_3:+60403072 None:intergenic
CACACCATACAGAGTCATGG+GGG 0.754927 4_3:+60404916 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAAAGATGATAAAATA+TGG + chr4_3:60404450-60404469 None:intergenic 10.0%
!!! CAAGTTTTGTATATATACTT+TGG + chr4_3:60403150-60403169 None:intergenic 20.0%
!!! TACATGCTTTTATATGAAAA+GGG - chr4_3:60403556-60403575 Msa0652700:CDS 20.0%
!! TATGTTAGTTCATAACATAA+AGG + chr4_3:60403683-60403702 None:intergenic 20.0%
!!! ACACATGTTTTTTTTTTGAA+GGG + chr4_3:60405071-60405090 None:intergenic 20.0%
!!! AACACATGTTTTTTTTTTGA+AGG + chr4_3:60405072-60405091 None:intergenic 20.0%
!! TCAAAAAAAAAACATGTGTT+TGG - chr4_3:60405072-60405091 Msa0652700:CDS 20.0%
!! ACCTCAAATCAATATTATTT+AGG + chr4_3:60405334-60405353 None:intergenic 20.0%
!! ACATTTTATCTGAATTTTGC+AGG - chr4_3:60402981-60403000 Msa0652700:intron 25.0%
!! TATATACTTTGGAAGATAGA+AGG + chr4_3:60403139-60403158 None:intergenic 25.0%
!! GTACATGCTTTTATATGAAA+AGG - chr4_3:60403555-60403574 Msa0652700:CDS 25.0%
!!! CTGTTCTGAGTTTTAAATTT+AGG - chr4_3:60403708-60403727 Msa0652700:intron 25.0%
!!! TTCTGAGTTTTAAATTTAGG+AGG - chr4_3:60403711-60403730 Msa0652700:intron 25.0%
!!! TCTGAGTTTTAAATTTAGGA+GGG - chr4_3:60403712-60403731 Msa0652700:intron 25.0%
!! GTATAATTTTGAACTGTTTC+AGG - chr4_3:60403959-60403978 Msa0652700:CDS 25.0%
! TTTAATATCTGTGTTACCTT+AGG + chr4_3:60404326-60404345 None:intergenic 25.0%
!!! TCTTGAACATTTTTTTTGAC+AGG - chr4_3:60404416-60404435 Msa0652700:intron 25.0%
!!! ACATTTTTTTTGACAGGTTT+CGG - chr4_3:60404422-60404441 Msa0652700:intron 25.0%
! TGTAATGATAAAACTTGCAA+GGG + chr4_3:60404835-60404854 None:intergenic 25.0%
! TTGTAATGATAAAACTTGCA+AGG + chr4_3:60404836-60404855 None:intergenic 25.0%
!! ACCTCTAATCAAATTTTTCA+CGG - chr4_3:60405119-60405138 Msa0652700:CDS 25.0%
! ACAACCCAAATAAAAAGATT+TGG - chr4_3:60405200-60405219 Msa0652700:CDS 25.0%
!!! CTGTCCAAATCTTTTTATTT+GGG + chr4_3:60405207-60405226 None:intergenic 25.0%
!! TCTGTCCAAATCTTTTTATT+TGG + chr4_3:60405208-60405227 None:intergenic 25.0%
!!! GCCTAAATAATATTGATTTG+AGG - chr4_3:60405330-60405349 Msa0652700:CDS 25.0%
! TGATGATAAGCAACAACTTT+TGG + chr4_3:60402030-60402049 None:intergenic 30.0%
! AGTTGTTGCTTATCATCAAT+AGG - chr4_3:60402032-60402051 Msa0652700:intron 30.0%
!! TGATTTCTGAAGCAAAAACA+AGG + chr4_3:60402165-60402184 None:intergenic 30.0%
AAATCATTCTCTCCTTCATT+TGG - chr4_3:60402179-60402198 Msa0652700:intron 30.0%
ATGTGTATCTTTCAGATGAT+GGG - chr4_3:60402392-60402411 Msa0652700:intron 30.0%
! GTGATAATAATGATTTTGCC+AGG - chr4_3:60402539-60402558 Msa0652700:CDS 30.0%
ATGTATGTTCTGCAATCTAA+AGG + chr4_3:60402633-60402652 None:intergenic 30.0%
! TCAAGAGATGGTATTACTTT+TGG - chr4_3:60402750-60402769 Msa0652700:CDS 30.0%
AAACTCAAATTATTGCTGTC+AGG + chr4_3:60402856-60402875 None:intergenic 30.0%
!! AGAAGATATCATTGATGATG+TGG - chr4_3:60403011-60403030 Msa0652700:intron 30.0%
AAATTGAACAAAAGACAAGG+AGG + chr4_3:60403351-60403370 None:intergenic 30.0%
AGGAAATTGAACAAAAGACA+AGG + chr4_3:60403354-60403373 None:intergenic 30.0%
CTAATGTTGTGAAATTTCTG+AGG + chr4_3:60403374-60403393 None:intergenic 30.0%
ACTGCAAAGATTTAATCTCA+AGG + chr4_3:60403460-60403479 None:intergenic 30.0%
!! ATGAAAAGGGTATCATTGTA+CGG - chr4_3:60403569-60403588 Msa0652700:CDS 30.0%
ATATGAAACTGGCACTAAAT+GGG - chr4_3:60403872-60403891 Msa0652700:intron 30.0%
!! AATTTTGAACTGTTTCAGGT+AGG - chr4_3:60403963-60403982 Msa0652700:CDS 30.0%
GGATTTATCTTGCATTGTAA+CGG - chr4_3:60404023-60404042 Msa0652700:CDS 30.0%
TTGAATGATGTACTAATCCA+AGG - chr4_3:60404107-60404126 Msa0652700:CDS 30.0%
! ATCTGAGATTGAAGTGAAAA+CGG + chr4_3:60404356-60404375 None:intergenic 30.0%
TTACTAGAAGTCTTCTTAAC+TGG - chr4_3:60404498-60404517 Msa0652700:intron 30.0%
TCAACTACTTGCAATCTATA+TGG - chr4_3:60404570-60404589 Msa0652700:CDS 30.0%
TTGCAATCTATATGGTTGTT+TGG - chr4_3:60404578-60404597 Msa0652700:CDS 30.0%
! GGAAGTCGTATTTATCATTT+TGG + chr4_3:60404689-60404708 None:intergenic 30.0%
CTATAAAAATGTTCGATGCT+CGG + chr4_3:60404710-60404729 None:intergenic 30.0%
! AAATATGTTGATTGTGAGGA+GGG + chr4_3:60404745-60404764 None:intergenic 30.0%
! AAAATATGTTGATTGTGAGG+AGG + chr4_3:60404746-60404765 None:intergenic 30.0%
! GACAAAATATGTTGATTGTG+AGG + chr4_3:60404749-60404768 None:intergenic 30.0%
! CACAATCAACATATTTTGTC+TGG - chr4_3:60404749-60404768 Msa0652700:intron 30.0%
! AACATATTTTGTCTGGTAGT+TGG - chr4_3:60404756-60404775 Msa0652700:intron 30.0%
TGGAGTTTATAGAGTGATAT+TGG - chr4_3:60404776-60404795 Msa0652700:CDS 30.0%
AGTTTATAGAGTGATATTGG+TGG - chr4_3:60404779-60404798 Msa0652700:CDS 30.0%
GTTTATAGAGTGATATTGGT+GGG - chr4_3:60404780-60404799 Msa0652700:CDS 30.0%
ATAAACTACCCTATCATTGA+AGG - chr4_3:60404855-60404874 Msa0652700:CDS 30.0%
TAAACTACCCTATCATTGAA+GGG - chr4_3:60404856-60404875 Msa0652700:CDS 30.0%
! AATGCCATTTAACATGTGTT+TGG - chr4_3:60405043-60405062 Msa0652700:CDS 30.0%
TCCGTGAAAAATTTGATTAG+AGG + chr4_3:60405123-60405142 None:intergenic 30.0%
! CTAATCAAATTTTTCACGGA+TGG - chr4_3:60405123-60405142 Msa0652700:CDS 30.0%
!! TCTTCAATGACATGTTTTTG+CGG + chr4_3:60405168-60405187 None:intergenic 30.0%
AACTGCAAGGTTATCATCTA+GGG - chr4_3:60401956-60401975 Msa0652700:CDS 35.0%
! TAAGCAACAACTTTTGGACA+TGG + chr4_3:60402024-60402043 None:intergenic 35.0%
TAGGCTTCTTGTTCTATTAC+AGG - chr4_3:60402083-60402102 Msa0652700:intron 35.0%
AGGCTTCTTGTTCTATTACA+GGG - chr4_3:60402084-60402103 Msa0652700:intron 35.0%
! TTTTCCAAGACCAAGAAAAC+AGG - chr4_3:60402116-60402135 Msa0652700:intron 35.0%
TTTCCAAGACCAAGAAAACA+GGG - chr4_3:60402117-60402136 Msa0652700:intron 35.0%
ATATGAACACATCAACAGCA+GGG + chr4_3:60402282-60402301 None:intergenic 35.0%
! TCTAAAGTGGGTTCCTTAAT+TGG + chr4_3:60402316-60402335 None:intergenic 35.0%
!! GTGTTCATCACTTCTAAAGT+GGG + chr4_3:60402328-60402347 None:intergenic 35.0%
! AGTGTTCATCACTTCTAAAG+TGG + chr4_3:60402329-60402348 None:intergenic 35.0%
! GAACACTGTTTTATCATCCA+TGG - chr4_3:60402342-60402361 Msa0652700:intron 35.0%
TACACATGAATCTTCTGTTG+AGG + chr4_3:60402379-60402398 None:intergenic 35.0%
CATGTGTATCTTTCAGATGA+TGG - chr4_3:60402391-60402410 Msa0652700:intron 35.0%
! GAAGGTACAGAATAAAGAAC+AGG - chr4_3:60402485-60402504 Msa0652700:CDS 35.0%
! CAATTTGCAGAGAAAGTATG+AGG - chr4_3:60402683-60402702 Msa0652700:CDS 35.0%
AGAGACATTCAGAAAAGATG+AGG - chr4_3:60402722-60402741 Msa0652700:CDS 35.0%
AGTAATACCATCTCTTGAGA+AGG + chr4_3:60402748-60402767 None:intergenic 35.0%
AGATCATTCATCAGTCATTG+AGG - chr4_3:60402773-60402792 Msa0652700:CDS 35.0%
TGTCTAGTATAAAGTGCAGA+TGG + chr4_3:60402962-60402981 None:intergenic 35.0%
! TACTTTGGAAGATAGAAGGA+TGG + chr4_3:60403135-60403154 None:intergenic 35.0%
AGGATGATATCTTTGGATCA+AGG + chr4_3:60403331-60403350 None:intergenic 35.0%
TTGAACAACTTCAGCTACAA+TGG - chr4_3:60403495-60403514 Msa0652700:CDS 35.0%
! TGTTTTACAAGCAACCTTGT+GGG + chr4_3:60403619-60403638 None:intergenic 35.0%
! CTGCAACTTCGAGAATATTT+TGG - chr4_3:60403744-60403763 Msa0652700:intron 35.0%
!!! TACACAAGAGTTTTTTTCCC+AGG - chr4_3:60403804-60403823 Msa0652700:CDS 35.0%
GCTTCTTGTAGATATGAAAC+TGG - chr4_3:60403861-60403880 Msa0652700:intron 35.0%
GATATGAAACTGGCACTAAA+TGG - chr4_3:60403871-60403890 Msa0652700:intron 35.0%
TATGAAACTGGCACTAAATG+GGG - chr4_3:60403873-60403892 Msa0652700:intron 35.0%
AATGCAAGATAAATCCAGTC+AGG + chr4_3:60404019-60404038 None:intergenic 35.0%
! TTATCTTGCATTGTAACGGA+TGG - chr4_3:60404027-60404046 Msa0652700:CDS 35.0%
!! TACTCTGGTTTGTTCGAAAA+CGG - chr4_3:60404140-60404159 Msa0652700:intron 35.0%
AAAGGGGCAAACAATAAAGA+GGG + chr4_3:60404250-60404269 None:intergenic 35.0%
CATTTAGTAGACAGAACTGA+GGG + chr4_3:60404289-60404308 None:intergenic 35.0%
TGTGTTACCTTAGGATACAA+GGG + chr4_3:60404317-60404336 None:intergenic 35.0%
GATGCTTTACTGAAGAAAGT+TGG - chr4_3:60404600-60404619 Msa0652700:CDS 35.0%
CTTACCTACTAATAAAGCAG+AGG - chr4_3:60404641-60404660 Msa0652700:CDS 35.0%
! TCATCCTCTGCTTTATTAGT+AGG + chr4_3:60404648-60404667 None:intergenic 35.0%
GGTTCCAAACACATGTTAAA+TGG + chr4_3:60405050-60405069 None:intergenic 35.0%
! TTTTGCGGTCATAAAACATC+TGG + chr4_3:60405153-60405172 None:intergenic 35.0%
TGTCATTGAAGAGTGATACT+TGG - chr4_3:60405176-60405195 Msa0652700:CDS 35.0%
GAACTGCAAGGTTATCATCT+AGG - chr4_3:60401955-60401974 Msa0652700:CDS 40.0%
GGTTATCATCTAGGGAGAAA+TGG - chr4_3:60401964-60401983 Msa0652700:CDS 40.0%
TATCATCTAGGGAGAAATGG+AGG - chr4_3:60401967-60401986 Msa0652700:CDS 40.0%
TTCCAAGACCAAGAAAACAG+GGG - chr4_3:60402118-60402137 Msa0652700:intron 40.0%
TGGGAAGACATAACGTGAAA+CGG + chr4_3:60402233-60402252 None:intergenic 40.0%
CATATGAACACATCAACAGC+AGG + chr4_3:60402283-60402302 None:intergenic 40.0%
ATGCACTGCAGATCCAATTA+AGG - chr4_3:60402300-60402319 Msa0652700:intron 40.0%
TTGAGGATAATCTAGTGCCA+TGG + chr4_3:60402362-60402381 None:intergenic 40.0%
ATTCTGTACCTTCTGCAGAA+GGG + chr4_3:60402478-60402497 None:intergenic 40.0%
TATTCTGTACCTTCTGCAGA+AGG + chr4_3:60402479-60402498 None:intergenic 40.0%
AAAGCAGAAAAGTAGGCCTT+GGG + chr4_3:60402514-60402533 None:intergenic 40.0%
ACTCTCCAAAGCAGAAAAGT+AGG + chr4_3:60402521-60402540 None:intergenic 40.0%
GGCAGAGAAGCAAAAGATTA+AGG - chr4_3:60402576-60402595 Msa0652700:CDS 40.0%
CTCGAAACATAGACAAGTTG+TGG + chr4_3:60403051-60403070 None:intergenic 40.0%
CAATCCAGAACTAGGATGTA+GGG + chr4_3:60403257-60403276 None:intergenic 40.0%
ACAATCCAGAACTAGGATGT+AGG + chr4_3:60403258-60403277 None:intergenic 40.0%
ACATGTCACAATCCAGAACT+AGG + chr4_3:60403265-60403284 None:intergenic 40.0%
ATGGCATATCGAGCTGAATT+TGG + chr4_3:60403299-60403318 None:intergenic 40.0%
TGGATCAAGGTGAAAACACA+TGG + chr4_3:60403318-60403337 None:intergenic 40.0%
GACAAGGAGGATGATATCTT+TGG + chr4_3:60403338-60403357 None:intergenic 40.0%
AACTTCAGCTACAATGGCAA+GGG - chr4_3:60403501-60403520 Msa0652700:CDS 40.0%
AAGCATGTACCTGACAATAC+AGG + chr4_3:60403545-60403564 None:intergenic 40.0%
TACGGAAATCAAGCGAATGA+AGG - chr4_3:60403587-60403606 Msa0652700:CDS 40.0%
!! AATGAAGGTATTGACCCACA+AGG - chr4_3:60403602-60403621 Msa0652700:CDS 40.0%
! GTGTTTTACAAGCAACCTTG+TGG + chr4_3:60403620-60403639 None:intergenic 40.0%
AATATTCTCGAAGTTGCAGC+AGG + chr4_3:60403743-60403762 None:intergenic 40.0%
!!! CAAGAGTTTTTTTCCCAGGA+CGG - chr4_3:60403808-60403827 Msa0652700:CDS 40.0%
!! GCATTGTAACGGATGGAATT+CGG - chr4_3:60404034-60404053 Msa0652700:CDS 40.0%
AATTTGTCGTAGCTGTACCA+AGG + chr4_3:60404088-60404107 None:intergenic 40.0%
! TACTAATCCAAGGCACTAGA+TGG - chr4_3:60404117-60404136 Msa0652700:intron 40.0%
!! ACTCTGGTTTGTTCGAAAAC+GGG - chr4_3:60404141-60404160 Msa0652700:intron 40.0%
TTAAAGGCCCATATATGAGG+GGG + chr4_3:60404181-60404200 None:intergenic 40.0%
CTTAAAGGCCCATATATGAG+GGG + chr4_3:60404182-60404201 None:intergenic 40.0%
CCTTAAAGGCCCATATATGA+GGG + chr4_3:60404183-60404202 None:intergenic 40.0%
TCCTTAAAGGCCCATATATG+AGG + chr4_3:60404184-60404203 None:intergenic 40.0%
!! AAACACAGACTTTGGAGTAG+TGG + chr4_3:60404209-60404228 None:intergenic 40.0%
CAAAGGGGCAAACAATAAAG+AGG + chr4_3:60404251-60404270 None:intergenic 40.0%
CTCTTTATTGTTTGCCCCTT+TGG - chr4_3:60404249-60404268 Msa0652700:CDS 40.0%
CCATTTAGTAGACAGAACTG+AGG + chr4_3:60404290-60404309 None:intergenic 40.0%
CCTCAGTTCTGTCTACTAAA+TGG - chr4_3:60404287-60404306 Msa0652700:CDS 40.0%
TGGAATACCCTTGTATCCTA+AGG - chr4_3:60404307-60404326 Msa0652700:CDS 40.0%
CTGTGTTACCTTAGGATACA+AGG + chr4_3:60404318-60404337 None:intergenic 40.0%
GAGATTGAAGTGAAAACGGT+AGG + chr4_3:60404352-60404371 None:intergenic 40.0%
CTAGAAGTCTTCTTAACTGG+AGG - chr4_3:60404501-60404520 Msa0652700:intron 40.0%
CTGGAGGAACACTACATAAA+TGG - chr4_3:60404517-60404536 Msa0652700:intron 40.0%
! AGTGATATTGGTGGGTGATT+GGG - chr4_3:60404788-60404807 Msa0652700:CDS 40.0%
TTGGGACCAAATGTTCATAC+AGG - chr4_3:60404806-60404825 Msa0652700:CDS 40.0%
! GATTAGAGGTGCAGAAGATT+GGG + chr4_3:60405109-60405128 None:intergenic 40.0%
! TGATTAGAGGTGCAGAAGAT+TGG + chr4_3:60405110-60405129 None:intergenic 40.0%
GAGAAATGGAGGTAACCCAA+GGG - chr4_3:60401978-60401997 Msa0652700:intron 45.0%
ATTGAGAGGAAGAGTCCCTT+GGG + chr4_3:60401996-60402015 None:intergenic 45.0%
TATTGAGAGGAAGAGTCCCT+TGG + chr4_3:60401997-60402016 None:intergenic 45.0%
! CAACAACTTTTGGACATGGC+AGG + chr4_3:60402020-60402039 None:intergenic 45.0%
ACACATGCTCCTTCATTCCA+TGG - chr4_3:60402057-60402076 Msa0652700:intron 45.0%
! TTCCCCTGTTTTCTTGGTCT+TGG + chr4_3:60402123-60402142 None:intergenic 45.0%
! AATGGCTTCCCCTGTTTTCT+TGG + chr4_3:60402129-60402148 None:intergenic 45.0%
CAAGGAGCCATGGTAACAAA+TGG + chr4_3:60402147-60402166 None:intergenic 45.0%
GAAGCAAAAACAAGGAGCCA+TGG + chr4_3:60402157-60402176 None:intergenic 45.0%
! CTCTCCTTCATTTGGTTCCT+TGG - chr4_3:60402187-60402206 Msa0652700:intron 45.0%
TTGGCCAAGGAACCAAATGA+AGG + chr4_3:60402194-60402213 None:intergenic 45.0%
GGGAAGACATAACGTGAAAC+GGG + chr4_3:60402232-60402251 None:intergenic 45.0%
TCACACCATACAGAGTCATG+GGG + chr4_3:60402423-60402442 None:intergenic 45.0%
CTCACACCATACAGAGTCAT+GGG + chr4_3:60402424-60402443 None:intergenic 45.0%
AATAAAGAACAGGTGCCCCA+AGG - chr4_3:60402495-60402514 Msa0652700:CDS 45.0%
AAGCAGAAAAGTAGGCCTTG+GGG + chr4_3:60402513-60402532 None:intergenic 45.0%
CAAAGCAGAAAAGTAGGCCT+TGG + chr4_3:60402515-60402534 None:intergenic 45.0%
!! CAAGGCCTACTTTTCTGCTT+TGG - chr4_3:60402513-60402532 Msa0652700:CDS 45.0%
TGCCAGGAGTAGTGTTTACA+TGG - chr4_3:60402555-60402574 Msa0652700:CDS 45.0%
TGCCATGTAAACACTACTCC+TGG + chr4_3:60402560-60402579 None:intergenic 45.0%
TTCTCCCTACATCCTAGTTC+TGG - chr4_3:60403250-60403269 Msa0652700:CDS 45.0%
CAACTTCAGCTACAATGGCA+AGG - chr4_3:60403500-60403519 Msa0652700:CDS 45.0%
CTTAAGGGACCTGTATTGTC+AGG - chr4_3:60403533-60403552 Msa0652700:CDS 45.0%
CTACAAGAAGCCAACAAGTG+AGG + chr4_3:60403852-60403871 None:intergenic 45.0%
GTAGTCGAAGATTTCCTGAC+TGG - chr4_3:60404002-60404021 Msa0652700:CDS 45.0%
GTTCGAAAACGGGATAAGCA+AGG - chr4_3:60404151-60404170 Msa0652700:intron 45.0%
AAGGTTTGCCCCCTCATATA+TGG - chr4_3:60404170-60404189 Msa0652700:intron 45.0%
AGGTTTGCCCCCTCATATAT+GGG - chr4_3:60404171-60404190 Msa0652700:intron 45.0%
CCCTCATATATGGGCCTTTA+AGG - chr4_3:60404180-60404199 Msa0652700:intron 45.0%
TGGAGTAGTGGCATCCTTAA+AGG + chr4_3:60404197-60404216 None:intergenic 45.0%
GCTCTGCAAAACACAGACTT+TGG + chr4_3:60404217-60404236 None:intergenic 45.0%
ATGGTGGCAAAACACCAAAG+GGG + chr4_3:60404266-60404285 None:intergenic 45.0%
! GATGGTGGCAAAACACCAAA+GGG + chr4_3:60404267-60404286 None:intergenic 45.0%
TAGTAGACAGAACTGAGGGA+TGG + chr4_3:60404285-60404304 None:intergenic 45.0%
GGATCAATGAGCAGAGAATC+TGG - chr4_3:60404538-60404557 Msa0652700:intron 45.0%
! GAGTGATATTGGTGGGTGAT+TGG - chr4_3:60404787-60404806 Msa0652700:CDS 45.0%
GGGCAACCTGTATGAACATT+TGG + chr4_3:60404815-60404834 None:intergenic 45.0%
TCACCAGCCCTTCAATGATA+GGG + chr4_3:60404866-60404885 None:intergenic 45.0%
ATCACCAGCCCTTCAATGAT+AGG + chr4_3:60404867-60404886 None:intergenic 45.0%
! TCATTGAAGGGCTGGTGATA+AGG - chr4_3:60404868-60404887 Msa0652700:CDS 45.0%
CTGGTGATAAGGAAGGACAA+GGG - chr4_3:60404879-60404898 Msa0652700:CDS 45.0%
GGAGAAATGGAGGTAACCCA+AGG - chr4_3:60401977-60401996 Msa0652700:intron 50.0%
!! TGGACATGGCAGGTATTGAG+AGG + chr4_3:60402010-60402029 None:intergenic 50.0%
CTCCTTCATTCCATGGCTCT+AGG - chr4_3:60402064-60402083 Msa0652700:intron 50.0%
AGCCTAGAGCCATGGAATGA+AGG + chr4_3:60402069-60402088 None:intergenic 50.0%
GAACAAGAAGCCTAGAGCCA+TGG + chr4_3:60402077-60402096 None:intergenic 50.0%
! GGGGAAGCCATTTGTTACCA+TGG - chr4_3:60402137-60402156 Msa0652700:intron 50.0%
CATCCTCTGGCAATCTCTCT+GGG + chr4_3:60402252-60402271 None:intergenic 50.0%
TCATCCTCTGGCAATCTCTC+TGG + chr4_3:60402253-60402272 None:intergenic 50.0%
TGTTCCCCCATGACTCTGTA+TGG - chr4_3:60402415-60402434 Msa0652700:intron 50.0%
CACACCATACAGAGTCATGG+GGG + chr4_3:60402422-60402441 None:intergenic 50.0%
CCTCACACCATACAGAGTCA+TGG + chr4_3:60402425-60402444 None:intergenic 50.0%
CCATGACTCTGTATGGTGTG+AGG - chr4_3:60402422-60402441 Msa0652700:intron 50.0%
GACTCTGTATGGTGTGAGGA+AGG - chr4_3:60402426-60402445 Msa0652700:intron 50.0%
TGTATGGTGTGAGGAAGGCT+TGG - chr4_3:60402431-60402450 Msa0652700:intron 50.0%
GTATGGTGTGAGGAAGGCTT+GGG - chr4_3:60402432-60402451 Msa0652700:intron 50.0%
GATGAGGCCTTCTCAAGAGA+TGG - chr4_3:60402738-60402757 Msa0652700:CDS 50.0%
AGTCCCCACCATTTCAAAGC+TGG - chr4_3:60403087-60403106 Msa0652700:CDS 50.0%
!! GCTCCAGCTTTGAAATGGTG+GGG + chr4_3:60403093-60403112 None:intergenic 50.0%
!! GGCTCCAGCTTTGAAATGGT+GGG + chr4_3:60403094-60403113 None:intergenic 50.0%
!! TGAGGGCTCCAGCTTTGAAA+TGG + chr4_3:60403098-60403117 None:intergenic 50.0%
GAGCCCTCAATGTCCTTGTA+CGG - chr4_3:60403109-60403128 Msa0652700:intron 50.0%
TGGCCGTACAAGGACATTGA+GGG + chr4_3:60403115-60403134 None:intergenic 50.0%
ATGGCCGTACAAGGACATTG+AGG + chr4_3:60403116-60403135 None:intergenic 50.0%
GATAGAAGGATGGCCGTACA+AGG + chr4_3:60403125-60403144 None:intergenic 50.0%
TCAGCTACAATGGCAAGGGA+TGG - chr4_3:60403505-60403524 Msa0652700:CDS 50.0%
CTACAATGGCAAGGGATGGA+CGG - chr4_3:60403509-60403528 Msa0652700:CDS 50.0%
TACAATGGCAAGGGATGGAC+GGG - chr4_3:60403510-60403529 Msa0652700:CDS 50.0%
CGAGCAATGTATTCCGTCCT+GGG + chr4_3:60403824-60403843 None:intergenic 50.0%
TCGAGCAATGTATTCCGTCC+TGG + chr4_3:60403825-60403844 None:intergenic 50.0%
GCTCGAAGAACCTCACTTGT+TGG - chr4_3:60403839-60403858 Msa0652700:intron 50.0%
! CGGTTTTCTGCGAACCATCT+AGG - chr4_3:60404054-60404073 Msa0652700:CDS 50.0%
GTAGCTGTACCAAGGAACTG+TGG + chr4_3:60404080-60404099 None:intergenic 50.0%
! CAGAGTACCATCTAGTGCCT+TGG + chr4_3:60404127-60404146 None:intergenic 50.0%
! CAAGGCACTAGATGGTACTC+TGG - chr4_3:60404125-60404144 Msa0652700:intron 50.0%
! GGATGGTGGCAAAACACCAA+AGG + chr4_3:60404268-60404287 None:intergenic 50.0%
TAGACAGAACTGAGGGATGG+TGG + chr4_3:60404282-60404301 None:intergenic 50.0%
ATTGTGAGGAGGGCAAGCAT+TGG + chr4_3:60404735-60404754 None:intergenic 50.0%
CTACCCTATCATTGAAGGGC+TGG - chr4_3:60404860-60404879 Msa0652700:CDS 50.0%
! TGAAGGGCTGGTGATAAGGA+AGG - chr4_3:60404872-60404891 Msa0652700:CDS 50.0%
GCTGGTGATAAGGAAGGACA+AGG - chr4_3:60404878-60404897 Msa0652700:CDS 50.0%
CAAGGGACGCATTTCACAGT+CGG - chr4_3:60404896-60404915 Msa0652700:CDS 50.0%
TGCAGAAGATTGGGAGGCTA+TGG + chr4_3:60405100-60405119 None:intergenic 50.0%
! TAGAGGTGCAGAAGATTGGG+AGG + chr4_3:60405106-60405125 None:intergenic 50.0%
TCCTTGGCCAAGCTTATCGC+TGG - chr4_3:60402203-60402222 Msa0652700:intron 55.0%
CTTCCCAGAGAGATTGCCAG+AGG - chr4_3:60402246-60402265 Msa0652700:intron 55.0%
GGAAGGCTTGGGAGTTTGCA+AGG - chr4_3:60402443-60402462 Msa0652700:CDS 55.0%
AGGCTTGGGAGTTTGCAAGG+TGG - chr4_3:60402446-60402465 Msa0652700:CDS 55.0%
CTTGGGAGTTTGCAAGGTGG+TGG - chr4_3:60402449-60402468 Msa0652700:CDS 55.0%
!! GGGCTCCAGCTTTGAAATGG+TGG + chr4_3:60403095-60403114 None:intergenic 55.0%
! CAAGGGATGGACGGGCTTAA+GGG - chr4_3:60403518-60403537 Msa0652700:CDS 55.0%
CCAAGGAACTGTGGCCTAGA+TGG + chr4_3:60404071-60404090 None:intergenic 55.0%
CCATCTAGGCCACAGTTCCT+TGG - chr4_3:60404068-60404087 Msa0652700:CDS 55.0%
GCCAGCGATAAGCTTGGCCA+AGG + chr4_3:60402207-60402226 None:intergenic 60.0%
GCAGGGAGCTTGTCATCCTC+TGG + chr4_3:60402265-60402284 None:intergenic 60.0%
GGTGGCTTCCCTTCTGCAGA+AGG - chr4_3:60402467-60402486 Msa0652700:CDS 60.0%
! GCAAGGGATGGACGGGCTTA+AGG - chr4_3:60403517-60403536 Msa0652700:CDS 60.0%
CGGGACGCCAGCGATAAGCT+TGG + chr4_3:60402213-60402232 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 60401954 60405403 60401954 ID=Msa0652700;Name=Msa0652700
chr4_3 mRNA 60401954 60405403 60401954 ID=Msa0652700-mRNA-1;Parent=Msa0652700;Name=Msa0652700-mRNA-1;_AED=0.13;_eAED=0.13;_QI=29|0.77|0.6|0.9|1|1|10|0|701
chr4_3 exon 60401954 60401992 60401954 ID=Msa0652700-mRNA-1:exon:16472;Parent=Msa0652700-mRNA-1
chr4_3 exon 60402440 60402919 60402440 ID=Msa0652700-mRNA-1:exon:16471;Parent=Msa0652700-mRNA-1
chr4_3 exon 60403029 60403123 60403029 ID=Msa0652700-mRNA-1:exon:16470;Parent=Msa0652700-mRNA-1
chr4_3 exon 60403211 60403376 60403211 ID=Msa0652700-mRNA-1:exon:16469;Parent=Msa0652700-mRNA-1
chr4_3 exon 60403455 60403627 60403455 ID=Msa0652700-mRNA-1:exon:16468;Parent=Msa0652700-mRNA-1
chr4_3 exon 60403749 60403848 60403749 ID=Msa0652700-mRNA-1:exon:16467;Parent=Msa0652700-mRNA-1
chr4_3 exon 60403921 60404133 60403921 ID=Msa0652700-mRNA-1:exon:16466;Parent=Msa0652700-mRNA-1
chr4_3 exon 60404232 60404354 60404232 ID=Msa0652700-mRNA-1:exon:16465;Parent=Msa0652700-mRNA-1
chr4_3 exon 60404563 60404664 60404563 ID=Msa0652700-mRNA-1:exon:16464;Parent=Msa0652700-mRNA-1
chr4_3 exon 60404760 60405403 60404760 ID=Msa0652700-mRNA-1:exon:16463;Parent=Msa0652700-mRNA-1
chr4_3 five_prime_UTR 60405375 60405403 60405375 ID=Msa0652700-mRNA-1:five_prime_utr;Parent=Msa0652700-mRNA-1
chr4_3 CDS 60404760 60405374 60404760 ID=Msa0652700-mRNA-1:cds;Parent=Msa0652700-mRNA-1
chr4_3 CDS 60404563 60404664 60404563 ID=Msa0652700-mRNA-1:cds;Parent=Msa0652700-mRNA-1
chr4_3 CDS 60404232 60404354 60404232 ID=Msa0652700-mRNA-1:cds;Parent=Msa0652700-mRNA-1
chr4_3 CDS 60403921 60404133 60403921 ID=Msa0652700-mRNA-1:cds;Parent=Msa0652700-mRNA-1
chr4_3 CDS 60403749 60403848 60403749 ID=Msa0652700-mRNA-1:cds;Parent=Msa0652700-mRNA-1
chr4_3 CDS 60403455 60403627 60403455 ID=Msa0652700-mRNA-1:cds;Parent=Msa0652700-mRNA-1
chr4_3 CDS 60403211 60403376 60403211 ID=Msa0652700-mRNA-1:cds;Parent=Msa0652700-mRNA-1
chr4_3 CDS 60403029 60403123 60403029 ID=Msa0652700-mRNA-1:cds;Parent=Msa0652700-mRNA-1
chr4_3 CDS 60402440 60402919 60402440 ID=Msa0652700-mRNA-1:cds;Parent=Msa0652700-mRNA-1
chr4_3 CDS 60401954 60401992 60401954 ID=Msa0652700-mRNA-1:cds;Parent=Msa0652700-mRNA-1
Gene Sequence

>Msa0652700

ATGGAGGTAACCCAAGGGACTCTTCCTCTCAATACCTGCCATGTCCAAAAGTTGTTGCTTATCATCAATAGGTTACACATGCTCCTTCATTCCATGGCTCTAGGCTTCTTGTTCTATTACAGGGTGTGCTTTCTTTTCCAAGACCAAGAAAACAGGGGAAGCCATTTGTTACCATGGCTCCTTGTTTTTGCTTCAGAAATCATTCTCTCCTTCATTTGGTTCCTTGGCCAAGCTTATCGCTGGCGTCCCGTTTCACGTTATGTCTTCCCAGAGAGATTGCCAGAGGATGACAAGCTCCCTGCTGTTGATGTGTTCATATGCACTGCAGATCCAATTAAGGAACCCACTTTAGAAGTGATGAACACTGTTTTATCATCCATGGCACTAGATTATCCTCAACAGAAGATTCATGTGTATCTTTCAGATGATGGGTGTTCCCCCATGACTCTGTATGGTGTGAGGAAGGCTTGGGAGTTTGCAAGGTGGTGGCTTCCCTTCTGCAGAAGGTACAGAATAAAGAACAGGTGCCCCAAGGCCTACTTTTCTGCTTTGGAGAGTGATAATAATGATTTTGCCAGGAGTAGTGTTTACATGGCAGAGAAGCAAAAGATTAAGAGAAAGTATGAGGCTTTTAAAGAAGAGATAGAGACATTCAGAAAAGATGAGGCCTTCTCAAGAGATGGTATTACTTTTGGAGATCATTCATCAGTCATTGAGGTGATGCAAGAAGATATCATTGATGATGTGGATAATGTCAAAATGCCACAACTTGTCTATGTTTCGAGAGAGAGAAAGTCTTCTAGTCCCCACCATTTCAAAGCTGGAGCCCTCAATGTCCTTCTTCGTGTATCTGCTGTGATGAGTAATTCTCCCTACATCCTAGTTCTGGATTGTGACATGTTCTGCAACTATCCAAATTCAGCTCGATATGCCATGTGTTTTCACCTTGATCCAAAGATATCATCCTCCTTGTCTTTTGTTCAATTTCCTCAGAAATTTCACAACATTAGCAAGAAAGACATCTATGACAGTCAGCTGAGATCATTATTTACTCTACAATGGCAAGGGATGGACGGGCTTAAGGGACCTGTATTGTCAGGTACATGCTTTTATATGAAAAGGGTATCATTGTACGGAAATCAAGCGAATGAAGGAGGGACTGACCTGCTGCAACTTCGAGAATATTTTGGTTCATCGAATGAATTCATCAAATCACTTGCTCAAAACTACACAAGAGTTTTTTTCCCAGGACGGAATACATTGCTCGAAGAACCTCACTTGTTGGCTTCTTGTAGATATGAAACTGGCACTAAATGGGGACAAGATGTAGGTTTCTTATACGATAGTGTAGTCGAAGATTTCCTGACTGGATTTATCTTGCATTGTAACGGATGGAATTCGGTTTTCTGCGAACCATCTAGGCCACAGTTCCTTGGTACAGCTACGACAAATTTGAATGATGTACTAATCCAAGGCACTAGATGGTACTCTGAGCTCACATATTTCCCTCTTTATTGTTTGCCCCTTTGGTGTTTTGCCACCATCCCTCAGTTCTGTCTACTAAATGGAATACCCTTGTATCCTAAGGTTTCGGATCCATATTTTATCATCTTTTTATTCATATTTTTATCATCTCTTTCAAAACATTTACTAGAAGTCTTCTTAACTGGAGGAACACTACATAAATGGATCAATGAGCAGAGAATCTGGATGATGAAATCAACTACTTGCAATCTATATGGTTGTTTGGATGCTTTACTGAAGAAAGTTGGAATAAGACAAGCTAATTTCTTACCTACTAATAAAGCAGAGGATGATGAACAAACTCTGCTATACCAAAATGATAAATACGACTTCCGAGCATCGAACATTTTTATAGTTCCAATGCTTGCCCTCCTCACAATCAACATATTTTGTCTGGTAGTTGGAGTTTATAGAGTGATATTGGTGGGTGATTGGGACCAAATGTTCATACAGGTTGCCCTTGCAAGTTTTATCATTACAATAAACTACCCTATCATTGAAGGGCTGGTGATAAGGAAGGACAAGGGACGCATTTCACAGTCGAAGACTTACTCGTCATTGAAATGTGCACCATTATATTAG
Protein sequence

>Msa0652700

MEVTQGTLPLNTCHVQKLLLIINRLHMLLHSMALGFLFYYRVCFLFQDQENRGSHLLPWLLVFASEIILSFIWFLGQAYRWRPVSRYVFPERLPEDDKLPAVDVFICTADPIKEPTLEVMNTVLSSMALDYPQQKIHVYLSDDGCSPMTLYGVRKAWEFARWWLPFCRRYRIKNRCPKAYFSALESDNNDFARSSVYMAEKQKIKRKYEAFKEEIETFRKDEAFSRDGITFGDHSSVIEVMQEDIIDDVDNVKMPQLVYVSRERKSSSPHHFKAGALNVLLRVSAVMSNSPYILVLDCDMFCNYPNSARYAMCFHLDPKISSSLSFVQFPQKFHNISKKDIYDSQLRSLFTLQWQGMDGLKGPVLSGTCFYMKRVSLYGNQANEGGTDLLQLREYFGSSNEFIKSLAQNYTRVFFPGRNTLLEEPHLLASCRYETGTKWGQDVGFLYDSVVEDFLTGFILHCNGWNSVFCEPSRPQFLGTATTNLNDVLIQGTRWYSELTYFPLYCLPLWCFATIPQFCLLNGIPLYPKVSDPYFIIFLFIFLSSLSKHLLEVFLTGGTLHKWINEQRIWMMKSTTCNLYGCLDALLKKVGIRQANFLPTNKAEDDEQTLLYQNDKYDFRASNIFIVPMLALLTINIFCLVVGVYRVILVGDWDQMFIQVALASFIITINYPIIEGLVIRKDKGRISQSKTYSSLKCAPLY*