AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1465860


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1465860 MtrunA17_Chr4g0066711 96.552 116 4 0 7 122 72 187 4.15e-78 228
Msa1465860 MtrunA17_Chr7g0268681 38.182 110 63 3 7 116 78 182 7.42e-18 75.1
Msa1465860 MtrunA17_Chr2g0286341 37.168 113 68 1 7 119 78 187 7.64e-17 72.4
Msa1465860 MtrunA17_Chr4g0066721 32.584 89 58 1 7 95 88 174 3.81e-12 60.5
Msa1465860 MtrunA17_Chr4g0042551 30.973 113 71 3 7 116 106 214 2.55e-11 58.5
Msa1465860 MtrunA17_Chr7g0217691 34.483 116 71 4 6 119 74 186 6.69e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1465860 AT4G15610.2 60.204 98 39 0 3 100 19 116 2.60e-38 126
Msa1465860 AT4G15610.1 62.766 94 35 0 7 100 79 172 3.60e-37 125
Msa1465860 AT3G06390.1 53.191 94 41 1 2 92 80 173 1.51e-30 108
Msa1465860 AT4G15630.1 33.645 107 69 1 7 113 82 186 4.89e-16 70.9
Msa1465860 AT1G14160.1 33.645 107 69 2 7 113 98 202 3.23e-13 63.5
Msa1465860 AT4G15620.1 29.907 107 73 1 7 113 82 186 4.58e-13 62.8
Msa1465860 AT5G15290.1 35.556 90 55 2 7 95 75 162 2.26e-12 61.2

Find 42 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTAGTGTGGTACATGATTT+TGG 0.138071 tig0045658:+1537 Msa1465860:three_prime_UTR
AGCTGGGAGTGTTGCATATT+TGG 0.167909 tig0045658:+1148 Msa1465860:CDS
TTCTCCTCCATTTAATCTTC+TGG 0.173285 tig0045658:+982 Msa1465860:CDS
GTGGCTGCATTCTCTGTTGC+TGG 0.248825 tig0045658:+891 Msa1465860:CDS
TTGTCACTCTGTTGCTCATT+TGG 0.263577 tig0045658:+1276 Msa1465860:CDS
AAAATCTATCACATCTTGTT+TGG 0.283680 tig0045658:-1668 Msa1465860:intergenic
CCGCATCCCAGAAGATTAAA+TGG 0.293656 tig0045658:-989 Msa1465860:intergenic
GCTGGGAGTGTTGCATATTT+GGG 0.294630 tig0045658:+1149 Msa1465860:CDS
AGGGAAACAACCACACTAAT+TGG 0.296811 tig0045658:+1177 Msa1465860:CDS
GATTAAATGGAGGAGAAGTT+TGG 0.346130 tig0045658:-976 Msa1465860:intergenic
TCTCCTCCATTTAATCTTCT+GGG 0.353459 tig0045658:+983 Msa1465860:CDS
CAACTTTCTTCGAATCTACT+TGG 0.362939 tig0045658:-1330 Msa1465860:intergenic
TTTCTCTTGCAGGTGATGTT+GGG 0.364730 tig0045658:+1101 Msa1465860:intron
GGGATATTAGCTTCAGCAAC+AGG 0.379873 tig0045658:+1122 Msa1465860:CDS
AATTAATGTCAATGAGAATA+AGG 0.391869 tig0045658:-807 Msa1465860:intergenic
AAATCTATCACATCTTGTTT+GGG 0.404170 tig0045658:-1667 Msa1465860:intergenic
GCTTCAGCAACAGGCACAGC+TGG 0.420135 tig0045658:+1131 Msa1465860:CDS
TCTGTAGCAGTGGGATTGTT+TGG 0.423026 tig0045658:+1248 Msa1465860:CDS
AATTAAAATGCTCTTTAAAG+AGG 0.431280 tig0045658:-1693 Msa1465860:intergenic
TGGCTGCATTCTCTGTTGCT+GGG 0.437451 tig0045658:+892 Msa1465860:CDS
ATGAGAATAAGGTCTTAATA+TGG 0.454474 tig0045658:-796 Msa1465860:intergenic
ATTTCTCTTGCAGGTGATGT+TGG 0.456848 tig0045658:+1100 Msa1465860:intron
TGTTGCATATTTGGGTTTGA+AGG 0.466255 tig0045658:+1157 Msa1465860:CDS
AATATGACATGCTATGATAC+TGG 0.472959 tig0045658:-1623 Msa1465860:intergenic
AACTTTCTTCGAATCTACTT+GGG 0.503750 tig0045658:-1329 Msa1465860:intergenic
AGAATAAGGTCTTAATATGG+TGG 0.511006 tig0045658:-793 Msa1465860:intergenic
GTTGCATATTTGGGTTTGAA+GGG 0.522790 tig0045658:+1158 Msa1465860:CDS
GGTCTTAAACAAAGTCATCA+TGG 0.527885 tig0045658:-760 Msa1465860:intergenic
TTTGAGGTTTGGCTTTCGGA+TGG 0.533858 tig0045658:-952 Msa1465860:intergenic
TTCTCTTGCAGGTGATGTTG+GGG 0.535972 tig0045658:+1102 Msa1465860:intron
CCATTTAATCTTCTGGGATG+CGG 0.538436 tig0045658:+989 Msa1465860:CDS
TATTGGTGCCTCTGTAGCAG+TGG 0.549094 tig0045658:+1238 Msa1465860:CDS
TAATATGGTGGGAACATATG+TGG 0.556998 tig0045658:-781 Msa1465860:intergenic
AAACAATCCCACTGCTACAG+AGG 0.565188 tig0045658:-1246 Msa1465860:intergenic
ATTGGTGCCTCTGTAGCAGT+GGG 0.574405 tig0045658:+1239 Msa1465860:CDS
CTTCAGCAACAGGCACAGCT+GGG 0.583484 tig0045658:+1132 Msa1465860:CDS
GAATAAGGTCTTAATATGGT+GGG 0.584875 tig0045658:-792 Msa1465860:intergenic
AGTAGATTCGAAGAAAGTTG+TGG 0.613149 tig0045658:+1333 Msa1465860:exon
ATGTGTGTAATTATCTACAA+AGG 0.625603 tig0045658:+1492 Msa1465860:three_prime_UTR
AGATTGAATAATTAACCAAG+TGG 0.664798 tig0045658:+1587 Msa1465860:three_prime_UTR
TGCAAAACTTGTCATAGATG+TGG 0.673434 tig0045658:-1211 Msa1465850:intergenic
CATCCCAGAAGATTAAATGG+AGG 0.717011 tig0045658:-986 Msa1465850:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAATCTATTTTATATTTGT+GGG - tig0045658:1574-1593 Msa1465860:intergenic 15.0%
!!! TTCAATCTATTTTATATTTG+TGG - tig0045658:1575-1594 Msa1465860:intergenic 15.0%
!!! TCAATCTATTTTATATTTGT+GGG - tig0045658:1574-1593 Msa1465860:intergenic 15.0%
!!! TTCAATCTATTTTATATTTG+TGG - tig0045658:1575-1594 Msa1465860:intergenic 15.0%
!! AATTAATGTCAATGAGAATA+AGG - tig0045658:810-829 None:intergenic 20.0%
!! AAGGTAAAAATGGAATTAAA+AGG - tig0045658:1033-1052 Msa1465860:intergenic 20.0%
!! GATTATAATAGTATTTAGCA+AGG - tig0045658:1052-1071 Msa1465860:intergenic 20.0%
!!! TATTTTACATTCTATTAGTG+TGG + tig0045658:1524-1543 Msa1465860:three_prime_UTR 20.0%
!!! AATTAAAATGCTCTTTAAAG+AGG - tig0045658:1696-1715 Msa1465860:intergenic 20.0%
!! AATTAATGTCAATGAGAATA+AGG - tig0045658:810-829 Msa1465860:intergenic 20.0%
!! AAGGTAAAAATGGAATTAAA+AGG - tig0045658:1033-1052 Msa1465860:intergenic 20.0%
!! GATTATAATAGTATTTAGCA+AGG - tig0045658:1052-1071 Msa1465860:intergenic 20.0%
!!! TATTTTACATTCTATTAGTG+TGG + tig0045658:1524-1543 Msa1465860:three_prime_UTR 20.0%
!!! AATTAAAATGCTCTTTAAAG+AGG - tig0045658:1696-1715 Msa1465860:intergenic 20.0%
! ATGAGAATAAGGTCTTAATA+TGG - tig0045658:799-818 None:intergenic 25.0%
!! CAATAATCACAGATATTTTG+TGG + tig0045658:872-891 Msa1465860:CDS 25.0%
! AGTATTTAGCAAGGTAAAAA+TGG - tig0045658:1043-1062 Msa1465860:intergenic 25.0%
! ATCACTGGATTATGTTTAAA+AGG - tig0045658:1082-1101 Msa1465860:intergenic 25.0%
!! AAATTTTATGCCAATTAGTG+TGG - tig0045658:1190-1209 Msa1465860:intergenic 25.0%
! ATGTGTGTAATTATCTACAA+AGG + tig0045658:1492-1511 Msa1465860:three_prime_UTR 25.0%
! AGATTGAATAATTAACCAAG+TGG + tig0045658:1587-1606 Msa1465860:three_prime_UTR 25.0%
!! AAATCTATCACATCTTGTTT+GGG - tig0045658:1670-1689 Msa1465860:intergenic 25.0%
! AAAATCTATCACATCTTGTT+TGG - tig0045658:1671-1690 Msa1465860:intergenic 25.0%
! ATGAGAATAAGGTCTTAATA+TGG - tig0045658:799-818 Msa1465860:intergenic 25.0%
!! CAATAATCACAGATATTTTG+TGG + tig0045658:872-891 Msa1465860:CDS 25.0%
! AGTATTTAGCAAGGTAAAAA+TGG - tig0045658:1043-1062 Msa1465860:intergenic 25.0%
! ATCACTGGATTATGTTTAAA+AGG - tig0045658:1082-1101 Msa1465860:intergenic 25.0%
!! AAATTTTATGCCAATTAGTG+TGG - tig0045658:1190-1209 Msa1465860:intergenic 25.0%
! ATGTGTGTAATTATCTACAA+AGG + tig0045658:1492-1511 Msa1465860:three_prime_UTR 25.0%
! AGATTGAATAATTAACCAAG+TGG + tig0045658:1587-1606 Msa1465860:three_prime_UTR 25.0%
!! AAATCTATCACATCTTGTTT+GGG - tig0045658:1670-1689 Msa1465860:intergenic 25.0%
! AAAATCTATCACATCTTGTT+TGG - tig0045658:1671-1690 Msa1465860:intergenic 25.0%
GAATAAGGTCTTAATATGGT+GGG - tig0045658:795-814 None:intergenic 30.0%
AGAATAAGGTCTTAATATGG+TGG - tig0045658:796-815 None:intergenic 30.0%
AACTTTCTTCGAATCTACTT+GGG - tig0045658:1332-1351 Msa1465860:intergenic 30.0%
! ATTAGTGTGGTACATGATTT+TGG + tig0045658:1537-1556 Msa1465860:three_prime_UTR 30.0%
AATATGACATGCTATGATAC+TGG - tig0045658:1626-1645 Msa1465860:intergenic 30.0%
GAATAAGGTCTTAATATGGT+GGG - tig0045658:795-814 Msa1465860:intergenic 30.0%
AGAATAAGGTCTTAATATGG+TGG - tig0045658:796-815 Msa1465860:intergenic 30.0%
AACTTTCTTCGAATCTACTT+GGG - tig0045658:1332-1351 Msa1465860:intergenic 30.0%
! ATTAGTGTGGTACATGATTT+TGG + tig0045658:1537-1556 Msa1465860:three_prime_UTR 30.0%
AATATGACATGCTATGATAC+TGG - tig0045658:1626-1645 Msa1465860:intergenic 30.0%
GGTCTTAAACAAAGTCATCA+TGG - tig0045658:763-782 None:intergenic 35.0%
! TAATATGGTGGGAACATATG+TGG - tig0045658:784-803 None:intergenic 35.0%
!!! AGAAGTTTGGTTTTGAGGTT+TGG - tig0045658:966-985 Msa1465860:intergenic 35.0%
GATTAAATGGAGGAGAAGTT+TGG - tig0045658:979-998 Msa1465860:intergenic 35.0%
TTCTCCTCCATTTAATCTTC+TGG + tig0045658:982-1001 Msa1465860:CDS 35.0%
TCTCCTCCATTTAATCTTCT+GGG + tig0045658:983-1002 Msa1465860:CDS 35.0%
! TGTTGCATATTTGGGTTTGA+AGG + tig0045658:1157-1176 Msa1465860:CDS 35.0%
! GTTGCATATTTGGGTTTGAA+GGG + tig0045658:1158-1177 Msa1465860:CDS 35.0%
TGCAAAACTTGTCATAGATG+TGG - tig0045658:1214-1233 Msa1465860:intergenic 35.0%
! ACATCTATGACAAGTTTTGC+AGG + tig0045658:1213-1232 Msa1465860:CDS 35.0%
CAACTTTCTTCGAATCTACT+TGG - tig0045658:1333-1352 Msa1465860:intergenic 35.0%
AGTAGATTCGAAGAAAGTTG+TGG + tig0045658:1333-1352 Msa1465860:exon 35.0%
AACAAAGTCATCATGGATGA+AGG - tig0045658:756-775 Msa1465860:intergenic 35.0%
GGTCTTAAACAAAGTCATCA+TGG - tig0045658:763-782 Msa1465860:intergenic 35.0%
! TAATATGGTGGGAACATATG+TGG - tig0045658:784-803 Msa1465860:intergenic 35.0%
!!! AGAAGTTTGGTTTTGAGGTT+TGG - tig0045658:966-985 Msa1465860:intergenic 35.0%
GATTAAATGGAGGAGAAGTT+TGG - tig0045658:979-998 Msa1465860:intergenic 35.0%
TTCTCCTCCATTTAATCTTC+TGG + tig0045658:982-1001 Msa1465860:CDS 35.0%
TCTCCTCCATTTAATCTTCT+GGG + tig0045658:983-1002 Msa1465860:CDS 35.0%
! TGTTGCATATTTGGGTTTGA+AGG + tig0045658:1157-1176 Msa1465860:CDS 35.0%
! GTTGCATATTTGGGTTTGAA+GGG + tig0045658:1158-1177 Msa1465860:CDS 35.0%
TGCAAAACTTGTCATAGATG+TGG - tig0045658:1214-1233 Msa1465860:intergenic 35.0%
! ACATCTATGACAAGTTTTGC+AGG + tig0045658:1213-1232 Msa1465860:CDS 35.0%
CAACTTTCTTCGAATCTACT+TGG - tig0045658:1333-1352 Msa1465860:intergenic 35.0%
AGTAGATTCGAAGAAAGTTG+TGG + tig0045658:1333-1352 Msa1465860:exon 35.0%
!!! TGGTTTTGAGGTTTGGCTTT+CGG - tig0045658:959-978 Msa1465860:intergenic 40.0%
CATCCCAGAAGATTAAATGG+AGG - tig0045658:989-1008 Msa1465860:intergenic 40.0%
!! CCATTTAATCTTCTGGGATG+CGG + tig0045658:989-1008 Msa1465860:CDS 40.0%
AATCCAGTGATTTCTCTTGC+AGG + tig0045658:1091-1110 Msa1465860:intron 40.0%
ATTTCTCTTGCAGGTGATGT+TGG + tig0045658:1100-1119 Msa1465860:intron 40.0%
TTTCTCTTGCAGGTGATGTT+GGG + tig0045658:1101-1120 Msa1465860:intron 40.0%
AGGGAAACAACCACACTAAT+TGG + tig0045658:1177-1196 Msa1465860:CDS 40.0%
! GACAAGTTTTGCAGGCATAT+TGG + tig0045658:1221-1240 Msa1465860:CDS 40.0%
TTGTCACTCTGTTGCTCATT+TGG + tig0045658:1276-1295 Msa1465860:CDS 40.0%
!!! GGTTTTAAATCACAGCCACT+TGG - tig0045658:1605-1624 Msa1465860:intergenic 40.0%
!!! TGGTTTTGAGGTTTGGCTTT+CGG - tig0045658:959-978 Msa1465860:intergenic 40.0%
CATCCCAGAAGATTAAATGG+AGG - tig0045658:989-1008 Msa1465860:intergenic 40.0%
!! CCATTTAATCTTCTGGGATG+CGG + tig0045658:989-1008 Msa1465860:CDS 40.0%
AATCCAGTGATTTCTCTTGC+AGG + tig0045658:1091-1110 Msa1465860:intron 40.0%
ATTTCTCTTGCAGGTGATGT+TGG + tig0045658:1100-1119 Msa1465860:intron 40.0%
TTTCTCTTGCAGGTGATGTT+GGG + tig0045658:1101-1120 Msa1465860:intron 40.0%
AGGGAAACAACCACACTAAT+TGG + tig0045658:1177-1196 Msa1465860:CDS 40.0%
! GACAAGTTTTGCAGGCATAT+TGG + tig0045658:1221-1240 Msa1465860:CDS 40.0%
TTGTCACTCTGTTGCTCATT+TGG + tig0045658:1276-1295 Msa1465860:CDS 40.0%
!!! GGTTTTAAATCACAGCCACT+TGG - tig0045658:1605-1624 Msa1465860:intergenic 40.0%
!! TTTGAGGTTTGGCTTTCGGA+TGG - tig0045658:955-974 Msa1465860:intergenic 45.0%
!!! GGAGGAGAAGTTTGGTTTTG+AGG - tig0045658:971-990 Msa1465860:intergenic 45.0%
CCGCATCCCAGAAGATTAAA+TGG - tig0045658:992-1011 Msa1465860:intergenic 45.0%
TCACCTGCAAGAGAAATCAC+TGG - tig0045658:1097-1116 Msa1465860:intergenic 45.0%
TTCTCTTGCAGGTGATGTTG+GGG + tig0045658:1102-1121 Msa1465860:intron 45.0%
! GGGATATTAGCTTCAGCAAC+AGG + tig0045658:1122-1141 Msa1465860:CDS 45.0%
!! AGCTGGGAGTGTTGCATATT+TGG + tig0045658:1148-1167 Msa1465860:CDS 45.0%
!! GCTGGGAGTGTTGCATATTT+GGG + tig0045658:1149-1168 Msa1465860:CDS 45.0%
AAACAATCCCACTGCTACAG+AGG - tig0045658:1249-1268 Msa1465860:intergenic 45.0%
TCTGTAGCAGTGGGATTGTT+TGG + tig0045658:1248-1267 Msa1465860:CDS 45.0%
!! TTTGAGGTTTGGCTTTCGGA+TGG - tig0045658:955-974 Msa1465860:intergenic 45.0%
!!! GGAGGAGAAGTTTGGTTTTG+AGG - tig0045658:971-990 Msa1465860:intergenic 45.0%
CCGCATCCCAGAAGATTAAA+TGG - tig0045658:992-1011 Msa1465860:intergenic 45.0%
TCACCTGCAAGAGAAATCAC+TGG - tig0045658:1097-1116 Msa1465860:intergenic 45.0%
TTCTCTTGCAGGTGATGTTG+GGG + tig0045658:1102-1121 Msa1465860:intron 45.0%
! GGGATATTAGCTTCAGCAAC+AGG + tig0045658:1122-1141 Msa1465860:CDS 45.0%
!! AGCTGGGAGTGTTGCATATT+TGG + tig0045658:1148-1167 Msa1465860:CDS 45.0%
!! GCTGGGAGTGTTGCATATTT+GGG + tig0045658:1149-1168 Msa1465860:CDS 45.0%
AAACAATCCCACTGCTACAG+AGG - tig0045658:1249-1268 Msa1465860:intergenic 45.0%
TCTGTAGCAGTGGGATTGTT+TGG + tig0045658:1248-1267 Msa1465860:CDS 45.0%
TGGCTGCATTCTCTGTTGCT+GGG + tig0045658:892-911 Msa1465860:CDS 50.0%
! TATTGGTGCCTCTGTAGCAG+TGG + tig0045658:1238-1257 Msa1465860:CDS 50.0%
! ATTGGTGCCTCTGTAGCAGT+GGG + tig0045658:1239-1258 Msa1465860:CDS 50.0%
TGGCTGCATTCTCTGTTGCT+GGG + tig0045658:892-911 Msa1465860:CDS 50.0%
! TATTGGTGCCTCTGTAGCAG+TGG + tig0045658:1238-1257 Msa1465860:CDS 50.0%
! ATTGGTGCCTCTGTAGCAGT+GGG + tig0045658:1239-1258 Msa1465860:CDS 50.0%
GTGGCTGCATTCTCTGTTGC+TGG + tig0045658:891-910 Msa1465860:CDS 55.0%
CTTCAGCAACAGGCACAGCT+GGG + tig0045658:1132-1151 Msa1465860:CDS 55.0%
GTGGCTGCATTCTCTGTTGC+TGG + tig0045658:891-910 Msa1465860:CDS 55.0%
CTTCAGCAACAGGCACAGCT+GGG + tig0045658:1132-1151 Msa1465860:CDS 55.0%
GCTTCAGCAACAGGCACAGC+TGG + tig0045658:1131-1150 Msa1465860:CDS 60.0%
GCTTCAGCAACAGGCACAGC+TGG + tig0045658:1131-1150 Msa1465860:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0045658 gene 756 1722 756 ID=Msa1465860;Name=Msa1465860
tig0045658 mRNA 756 1722 756 ID=Msa1465860-mRNA-1;Parent=Msa1465860;Name=Msa1465860-mRNA-1;_AED=0.05;_eAED=0.05;_QI=111|1|1|1|1|1|2|385|122
tig0045658 exon 756 1010 756 ID=Msa1465860-mRNA-1:exon:24913;Parent=Msa1465860-mRNA-1
tig0045658 exon 1113 1722 1113 ID=Msa1465860-mRNA-1:exon:24914;Parent=Msa1465860-mRNA-1
tig0045658 five_prime_UTR 756 866 756 ID=Msa1465860-mRNA-1:five_prime_utr;Parent=Msa1465860-mRNA-1
tig0045658 CDS 867 1010 867 ID=Msa1465860-mRNA-1:cds;Parent=Msa1465860-mRNA-1
tig0045658 CDS 1113 1337 1113 ID=Msa1465860-mRNA-1:cds;Parent=Msa1465860-mRNA-1
tig0045658 three_prime_UTR 1338 1722 1338 ID=Msa1465860-mRNA-1:three_prime_utr;Parent=Msa1465860-mRNA-1
Gene Sequence

>Msa1465860

ATGTTCAATAATCACAGATATTTTGTGGCTGCATTCTCTGTTGCTGGGCTTTATAGTATCATTACAACTTTTGTATCACTCTCTGCCATCCGAAAGCCAAACCTCAAAACCAAACTTCTCCTCCATTTAATCTTCTGGGATGCGGTGATGTTGGGGATATTAGCTTCAGCAACAGGCACAGCTGGGAGTGTTGCATATTTGGGTTTGAAGGGAAACAACCACACTAATTGGCATAAAATTTGCCACATCTATGACAAGTTTTGCAGGCATATTGGTGCCTCTGTAGCAGTGGGATTGTTTGGTAGCATTGTCACTCTGTTGCTCATTTGGATTTCAGCTCACAGCATTCATAGTCGAGTTCCCAAGTAG

Protein sequence

>Msa1465860

MFNNHRYFVAAFSVAGLYSIITTFVSLSAIRKPNLKTKLLLHLIFWDAVMLGILASATGTAGSVAYLGLKGNNHTNWHKICHIYDKFCRHIGASVAVGLFGSIVTLLLIWISAHSIHSRVPK*