AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0036250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0036250 MtrunA17_Chr1g0198431 93.581 296 7 2 1 296 1 284 0.0 566
Msa0036250 MtrunA17_Chr1g0166671 44.915 118 63 2 56 171 52 169 1.22e-28 109
Msa0036250 MtrunA17_Chr1g0187251 33.793 145 95 1 55 198 70 214 7.50e-24 97.1
Msa0036250 MtrunA17_Chr7g0266751 47.191 89 40 1 198 286 33 114 5.86e-20 84.0
Msa0036250 MtrunA17_Chr6g0481071 44.048 84 40 1 198 281 96 172 3.34e-19 83.2
Msa0036250 MtrunA17_Chr7g0263001 44.211 95 46 1 192 286 30 117 1.08e-18 80.9
Msa0036250 MtrunA17_Chr4g0042161 41.379 87 44 1 199 285 53 132 1.84e-17 77.4
Msa0036250 MtrunA17_Chr2g0280611 27.957 186 131 1 1 183 1 186 6.30e-17 78.2
Msa0036250 MtrunA17_Chr4g0042151 37.778 90 46 2 199 288 46 125 3.26e-15 72.4
Msa0036250 MtrunA17_Chr4g0031091 42.169 83 41 1 198 280 34 109 1.31e-14 68.9
Msa0036250 MtrunA17_Chr4g0050781 41.667 84 42 2 198 281 8 84 1.72e-14 70.9
Msa0036250 MtrunA17_Chr3g0121631 36.782 87 51 1 179 261 18 104 2.60e-14 68.9
Msa0036250 MtrunA17_Chr7g0219031 36.842 95 58 1 175 269 20 112 3.75e-14 67.8
Msa0036250 MtrunA17_Chr4g0067641 29.752 121 84 1 54 173 58 178 5.43e-14 70.5
Msa0036250 MtrunA17_Chr2g0278661 40.476 84 43 2 198 281 8 84 5.96e-14 70.1
Msa0036250 MtrunA17_Chr2g0327921 40.476 84 43 1 198 281 8 84 6.57e-14 66.2
Msa0036250 MtrunA17_Chr3g0119311 45.312 64 35 0 198 261 37 100 9.35e-14 67.0
Msa0036250 MtrunA17_Chr7g0219021 35.294 102 51 2 193 294 36 122 1.09e-13 69.3
Msa0036250 MtrunA17_Chr4g0061451 33.696 92 54 1 193 284 38 122 2.68e-13 68.6
Msa0036250 MtrunA17_Chr6g0467031 34.737 95 61 1 83 176 74 168 4.19e-13 66.6
Msa0036250 MtrunA17_Chr3g0088511 34.000 100 56 2 196 295 31 120 9.46e-13 64.3
Msa0036250 MtrunA17_Chr4g0065741 37.624 101 45 2 181 281 66 148 1.04e-12 65.1
Msa0036250 MtrunA17_Chr3g0086861 40.000 70 42 0 192 261 39 108 1.48e-12 67.4
Msa0036250 MtrunA17_Chr2g0316311 30.400 125 86 1 67 191 78 201 1.52e-12 67.4
Msa0036250 MtrunA17_Chr4g0045921 44.048 84 39 1 197 280 76 151 3.36e-12 66.2
Msa0036250 MtrunA17_Chr8g0346011 45.455 66 36 0 198 263 38 103 3.02e-11 62.8
Msa0036250 MtrunA17_Chr7g0276531 36.585 82 48 1 184 261 5 86 4.87e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0036250 AT3G20930.1 69.266 218 54 3 78 295 167 371 1.05e-104 310
Msa0036250 AT3G20930.1 50.000 86 43 0 86 171 72 157 1.07e-26 108
Msa0036250 AT3G20930.2 67.713 223 54 4 78 295 167 376 1.62e-102 305
Msa0036250 AT3G20930.2 50.000 86 43 0 86 171 72 157 9.72e-27 108
Msa0036250 AT1G32580.1 43.478 115 64 1 58 171 66 180 3.51e-27 106
Msa0036250 AT2G35240.1 38.235 136 83 1 56 190 67 202 4.77e-26 103
Msa0036250 AT1G73530.1 44.330 97 44 2 198 294 78 164 1.15e-18 82.0
Msa0036250 AT4G13850.4 37.838 111 61 2 174 284 13 115 9.39e-17 75.5
Msa0036250 AT4G13850.3 38.261 115 61 3 174 286 13 119 1.02e-16 75.9
Msa0036250 AT4G13850.2 38.261 115 61 3 174 286 13 119 1.26e-16 75.9
Msa0036250 AT4G13850.1 38.261 115 61 3 174 286 13 119 1.41e-16 75.9
Msa0036250 AT1G11430.1 29.231 130 88 1 54 183 61 186 2.32e-16 77.0
Msa0036250 AT5G06210.1 39.286 112 56 4 173 281 9 111 4.63e-16 74.3
Msa0036250 AT3G23830.2 39.785 93 47 2 196 286 34 119 5.21e-16 73.6
Msa0036250 AT3G23830.1 39.785 93 47 2 196 286 34 119 5.21e-16 73.6
Msa0036250 AT2G37510.1 42.529 87 43 1 192 278 29 108 7.99e-16 73.6
Msa0036250 AT4G20030.1 44.048 84 40 1 198 281 41 117 2.51e-15 72.4
Msa0036250 AT2G27330.1 37.778 90 46 2 199 285 23 105 6.53e-15 70.1
Msa0036250 AT3G46020.1 40.476 84 43 1 198 281 8 84 1.23e-14 68.9
Msa0036250 AT5G54580.1 38.554 83 44 1 199 281 58 133 1.57e-14 70.1
Msa0036250 AT3G26420.1 40.476 84 43 1 198 281 8 84 7.86e-14 70.1
Msa0036250 AT5G47320.1 34.821 112 65 2 173 284 8 111 1.26e-13 68.9
Msa0036250 AT5G61030.2 38.235 68 42 0 194 261 37 104 3.84e-13 68.9
Msa0036250 AT5G61030.1 38.235 68 42 0 194 261 37 104 3.84e-13 68.9
Msa0036250 AT2G46780.1 39.806 103 56 2 194 293 19 118 5.82e-13 68.6
Msa0036250 AT2G46780.2 39.806 103 56 2 194 293 19 118 5.82e-13 68.6
Msa0036250 AT3G15000.1 33.645 107 70 1 67 173 80 185 1.17e-12 68.2
Msa0036250 AT1G74230.1 42.857 63 36 0 194 256 31 93 6.67e-12 65.1
Msa0036250 AT3G08000.1 43.750 64 36 0 198 261 42 105 1.49e-11 61.6
Msa0036250 AT3G06790.2 30.088 113 70 2 65 173 75 182 1.66e-11 63.5
Msa0036250 AT4G39260.3 34.118 85 49 1 198 282 7 84 1.76e-11 60.1
Msa0036250 AT2G22090.2 43.750 64 36 0 194 257 101 164 4.96e-11 63.2
Msa0036250 AT3G06790.1 29.204 113 71 2 65 173 75 182 5.26e-11 62.0
Msa0036250 AT2G22090.1 43.750 64 36 0 194 257 101 164 5.65e-11 62.8
Msa0036250 AT3G15010.1 36.709 79 50 0 185 263 63 141 6.24e-11 63.2
Msa0036250 AT3G15010.2 36.709 79 50 0 185 263 63 141 6.24e-11 63.2
Msa0036250 AT4G39260.2 39.062 64 39 0 198 261 7 70 6.95e-11 59.3
Msa0036250 AT1G22910.2 43.939 66 37 0 198 263 14 79 8.70e-11 61.2
Msa0036250 AT1G22910.1 43.939 66 37 0 198 263 14 79 9.63e-11 61.2

Find 102 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTCATGGAAAACCAATTT+TGG 0.112663 1_1:-64717500 Msa0036250:CDS
TTATTTGAAGTTGGTGAATT+TGG 0.196836 1_1:+64717917 None:intergenic
TACTTACTTTCCCATGATTT+TGG 0.210808 1_1:+64717654 None:intergenic
AGATCTATTTGCAAAATCTT+TGG 0.212636 1_1:+64714960 None:intergenic
TTCACAACAAAACCCAAAAT+TGG 0.220232 1_1:+64717487 None:intergenic
CATATGTTCAGGTAGAAATT+TGG 0.220805 1_1:-64716443 Msa0036250:intron
TGACTTGCTTCAGAAGAATT+TGG 0.222835 1_1:+64716403 None:intergenic
AAAATACTAAAATTTAATTT+TGG 0.239857 1_1:-64714803 Msa0036250:three_prime_UTR
CAATAATAAATCTTTAACTA+TGG 0.241661 1_1:-64714835 Msa0036250:three_prime_UTR
AACATCTCCGGAGAATATTA+TGG 0.243718 1_1:+64715178 None:intergenic
TTGTGCGTGCAGATTATTAA+TGG 0.253086 1_1:-64715755 Msa0036250:intron
AGAGGGAAGGGTTTGAGATT+TGG 0.267017 1_1:+64717960 None:intergenic
CAATGCTTTGAATTTCCATT+AGG 0.287884 1_1:+64717754 None:intergenic
TATTTGAAGTTGGTGAATTT+GGG 0.299578 1_1:+64717918 None:intergenic
GTATCTTGGTGGGGTGGTCT+TGG 0.320085 1_1:+64715709 None:intergenic
GATATGTTGTTGTTCCCTAA+TGG 0.320694 1_1:-64717769 Msa0036250:CDS
ATGTTTGTGACACAATTCTA+AGG 0.328272 1_1:+64718012 None:intergenic
GATCTATTTGCAAAATCTTT+GGG 0.333253 1_1:+64714961 None:intergenic
GCTTGGACGATGATGTTATA+AGG 0.335303 1_1:-64715317 Msa0036250:three_prime_UTR
TCTGTCCAACAGGCTTGTTC+TGG 0.336939 1_1:-64714891 Msa0036250:three_prime_UTR
AAATGTAAATGTAGGGATAT+TGG 0.346471 1_1:+64715018 None:intergenic
AATGCTTTGAATTTCCATTA+GGG 0.351537 1_1:+64717755 None:intergenic
CGCGCCGCATTTGAAGGCTT+TGG 0.352596 1_1:-64716112 Msa0036250:CDS
TGGTCAAAATTTGAGCATAA+TGG 0.352734 1_1:+64717674 None:intergenic
TGTGTATATATCATGTTTCA+TGG 0.359894 1_1:-64717514 Msa0036250:CDS
ATGGTCAACAATTTGAGCTT+TGG 0.361173 1_1:+64717693 None:intergenic
AAAAGAGGAATTACATGAAA+TGG 0.362195 1_1:+64717837 None:intergenic
GACACAATTCTAAGGTTTCA+TGG 0.371212 1_1:+64718020 None:intergenic
TTATAACCACACATGCTCTT+AGG 0.374255 1_1:-64715139 Msa0036250:three_prime_UTR
TCAGACTCAAAATTGTCATC+TGG 0.388385 1_1:+64717310 None:intergenic
GCACGACCCTTGTTGTATCT+TGG 0.392813 1_1:+64715695 None:intergenic
CGGTTTGTGGAATTGATGTT+TGG 0.393172 1_1:+64717791 None:intergenic
ACCTTACGCGCCGCATTTGA+AGG 0.398224 1_1:-64716118 Msa0036250:CDS
GATAGAGAAGTAACTTGGTC+AGG 0.399152 1_1:+64715279 None:intergenic
TTCTTATCAAGCAGATGGTC+TGG 0.401047 1_1:+64715673 None:intergenic
AAAGCATTGGGTTGTTAGAA+TGG 0.404538 1_1:-64717740 Msa0036250:CDS
CCTCTGACATCTTTATGAAA+TGG 0.404893 1_1:-64714931 Msa0036250:three_prime_UTR
TAATGGAAATTCAAAGCATT+GGG 0.410201 1_1:-64717752 Msa0036250:CDS
TGTCTTTGATTCATTCTCAA+TGG 0.412062 1_1:+64718065 None:intergenic
CTTGGACGATGATGTTATAA+GGG 0.423624 1_1:-64715316 Msa0036250:three_prime_UTR
TATGTTCAAAATGTAAATGT+AGG 0.426220 1_1:+64715010 None:intergenic
TGCTGAAATTCTAGTAAAAG+AGG 0.428882 1_1:+64717822 None:intergenic
TTAAAGATTTATTATTGTAC+AGG 0.432425 1_1:+64714841 None:intergenic
TGAAGATAAGTTGAATCAAA+TGG 0.435228 1_1:+64715225 None:intergenic
GATTGTGTTGCAGGTGTGCC+TGG 0.437556 1_1:-64717349 Msa0036250:intron
ACATCTCCGGAGAATATTAT+GGG 0.438627 1_1:+64715179 None:intergenic
AAATTCTTCTGAAGCAAGTC+AGG 0.439004 1_1:-64716401 Msa0036250:CDS
GCCTTCAAATGCGGCGCGTA+AGG 0.441311 1_1:+64716117 None:intergenic
CTAATGGAAATTCAAAGCAT+TGG 0.444153 1_1:-64717753 Msa0036250:CDS
ATTAGGGAACAACAACATAT+CGG 0.444939 1_1:+64717771 None:intergenic
TTCAATAAATGCATAACCCT+TGG 0.455487 1_1:+64715959 None:intergenic
CGACCCTTGTTGTATCTTGG+TGG 0.456934 1_1:+64715698 None:intergenic
TTATAGATAAAATTTCAAAA+AGG 0.469097 1_1:-64715983 Msa0036250:CDS
TTACATACATGAAAGAAAGA+TGG 0.469881 1_1:+64715879 None:intergenic
TTGCAAAATCTTTGGGCCAA+AGG 0.474574 1_1:+64714968 None:intergenic
TCTGAAAATAAGGATTATGA+AGG 0.486651 1_1:-64717292 Msa0036250:intron
CTCTTTGAGTGCTGCACTTG+CGG 0.491061 1_1:+64715920 None:intergenic
GACCCTTGTTGTATCTTGGT+GGG 0.491716 1_1:+64715699 None:intergenic
GATAAGAAAAGAAGTCTGTA+AGG 0.503154 1_1:-64715658 Msa0036250:intron
ATGCTTCATTCAACAAGGCT+TGG 0.523221 1_1:-64715334 Msa0036250:three_prime_UTR
TATTAATGGCTGGATGATAG+TGG 0.526032 1_1:-64715741 Msa0036250:CDS
GGCTTTGGTGAGCTTGTTGA+AGG 0.526097 1_1:-64716097 Msa0036250:intron
ACACAATTCTAAGGTTTCAT+GGG 0.541585 1_1:+64718021 None:intergenic
TTGAGATTTGGTGAATGTAA+CGG 0.542477 1_1:+64717972 None:intergenic
TTTGAAAGTTCAGTGTGTGC+AGG 0.552838 1_1:+64715048 None:intergenic
TTGGTAACAACACCAACAGC+AGG 0.553041 1_1:+64717712 None:intergenic
AACAACAACATATCGGTTTG+TGG 0.554681 1_1:+64717778 None:intergenic
TTGAATCAAATGGAGACAGA+GGG 0.557854 1_1:+64715235 None:intergenic
TGCACTTGCGGCCTCCTCTG+TGG 0.559303 1_1:+64715932 None:intergenic
ATGTTCAAAATGTAAATGTA+GGG 0.567495 1_1:+64715011 None:intergenic
CTTGTTGTATCTTGGTGGGG+TGG 0.570175 1_1:+64715703 None:intergenic
TCACTTCCTAAGAGCATGTG+TGG 0.571599 1_1:+64715133 None:intergenic
GCAGCACTCAAAGAGATGAA+TGG 0.574975 1_1:-64715912 Msa0036250:CDS
ATAGAGAAGTAACTTGGTCA+GGG 0.575323 1_1:+64715280 None:intergenic
TAGACACCCATAATATTCTC+CGG 0.577652 1_1:-64715185 Msa0036250:three_prime_UTR
GCCTACCAGAACAAGCCTGT+TGG 0.582157 1_1:+64714886 None:intergenic
GGCTGAACAGACAAGACACC+AGG 0.582996 1_1:+64717331 None:intergenic
TCCAACAGGCTTGTTCTGGT+AGG 0.584549 1_1:-64714887 Msa0036250:three_prime_UTR
GCGTGCAGATTATTAATGGC+TGG 0.585212 1_1:-64715751 Msa0036250:intron
GGTGTTTGTTTGCAGTGAGA+AGG 0.587961 1_1:-64717546 Msa0036250:intron
CACGTACCAGCGATCACTAG+AGG 0.589950 1_1:-64715099 Msa0036250:three_prime_UTR
GAAATGATAGAGAAGTAACT+TGG 0.590055 1_1:+64715274 None:intergenic
GATTCGCCTCTAGTGATCGC+TGG 0.591786 1_1:+64715093 None:intergenic
TCAAATGGAGACAGAGGGGA+AGG 0.593229 1_1:+64715240 None:intergenic
ACCCTTGTTGTATCTTGGTG+GGG 0.593877 1_1:+64715700 None:intergenic
CACCCCACCAAGATACAACA+AGG 0.594378 1_1:-64715702 Msa0036250:CDS
GATTGTGCACATGAGCTAGC+TGG 0.605037 1_1:-64717455 Msa0036250:intron
CTCACCAAAGCCTTCAAATG+CGG 0.607563 1_1:+64716108 None:intergenic
TTAGAATTGTGTCACAAACA+TGG 0.617191 1_1:-64718010 Msa0036250:exon
GGCGAATCTAGTGTATCATG+AGG 0.630993 1_1:-64715078 Msa0036250:three_prime_UTR
GTTGAATCAAATGGAGACAG+AGG 0.631897 1_1:+64715234 None:intergenic
CCATTTCATAAAGATGTCAG+AGG 0.632361 1_1:+64714931 None:intergenic
TATTGAATACACCACAGAGG+AGG 0.649244 1_1:-64715943 Msa0036250:CDS
TTGGACGATGATGTTATAAG+GGG 0.661225 1_1:-64715315 Msa0036250:three_prime_UTR
AGAATGGATAAACCTGCTGT+TGG 0.670334 1_1:-64717724 Msa0036250:CDS
AATGCATGCTTCATTCAACA+AGG 0.670821 1_1:-64715339 Msa0036250:three_prime_UTR
ACTCAAAGAGATGAATGGCA+AGG 0.678432 1_1:-64715907 Msa0036250:CDS
GCGAATCTAGTGTATCATGA+GGG 0.682810 1_1:-64715077 Msa0036250:three_prime_UTR
TAGAGAAGTAACTTGGTCAG+GGG 0.693220 1_1:+64715281 None:intergenic
TGAATCAAATGGAGACAGAG+GGG 0.733687 1_1:+64715236 None:intergenic
ACCCCACCAAGATACAACAA+GGG 0.762422 1_1:-64715701 Msa0036250:CDS
ATTTATTGAATACACCACAG+AGG 0.775447 1_1:-64715946 Msa0036250:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATAATATATTAGCAAAAA+CGG + chr1_1:64715627-64715646 None:intergenic 10.0%
!! AATATATTATAAATTTACCT+AGG - chr1_1:64715635-64715654 Msa0036250:intron 10.0%
!! TATGTTAATTTATATTTCAT+TGG - chr1_1:64715715-64715734 Msa0036250:CDS 10.0%
!! ATAAATAAATATGTATGTAT+TGG - chr1_1:64716091-64716110 Msa0036250:intron 10.0%
!! TTATAGATAAAATTTCAAAA+AGG - chr1_1:64716906-64716925 Msa0036250:intron 10.0%
!! AGATAAATAATTGAATTATA+TGG + chr1_1:64717370-64717389 None:intergenic 10.0%
!! TTAAAGATTTATTATTGTAC+AGG + chr1_1:64718051-64718070 None:intergenic 15.0%
!! CAATAATAAATCTTTAACTA+TGG - chr1_1:64718054-64718073 Msa0036250:five_prime_UTR 15.0%
!!! GTTTTGTTTTTATTTGAAGT+TGG + chr1_1:64714984-64715003 None:intergenic 20.0%
!! GAAGCATAAATAAATACTTT+GGG - chr1_1:64716668-64716687 Msa0036250:intron 20.0%
!!! CGCTTTTACTAATAAATAAA+TGG - chr1_1:64716861-64716880 Msa0036250:intron 20.0%
!!! ATTTTTATATCTTCCAGATT+TGG - chr1_1:64717516-64717535 Msa0036250:CDS 20.0%
!!! TTTTTATATCTTCCAGATTT+GGG - chr1_1:64717517-64717536 Msa0036250:CDS 20.0%
!! ATGTTCAAAATGTAAATGTA+GGG + chr1_1:64717881-64717900 None:intergenic 20.0%
!! TATGTTCAAAATGTAAATGT+AGG + chr1_1:64717882-64717901 None:intergenic 20.0%
!!! TATTTGAAGTTGGTGAATTT+GGG + chr1_1:64714974-64714993 None:intergenic 25.0%
!!! TTATTTGAAGTTGGTGAATT+TGG + chr1_1:64714975-64714994 None:intergenic 25.0%
!!! TTAGAGTGATTGTTTTTGTT+GGG + chr1_1:64715009-64715028 None:intergenic 25.0%
!!! ATTAGAGTGATTGTTTTTGT+TGG + chr1_1:64715010-64715029 None:intergenic 25.0%
! AAAAGAGGAATTACATGAAA+TGG + chr1_1:64715055-64715074 None:intergenic 25.0%
!! AATGCTTTGAATTTCCATTA+GGG + chr1_1:64715137-64715156 None:intergenic 25.0%
!! TAATGGAAATTCAAAGCATT+GGG - chr1_1:64715137-64715156 Msa0036250:three_prime_UTR 25.0%
!!! TCAAATTTTGACCAAAATCA+TGG - chr1_1:64715224-64715243 Msa0036250:three_prime_UTR 25.0%
!!! CAAATTTTGACCAAAATCAT+GGG - chr1_1:64715225-64715244 Msa0036250:three_prime_UTR 25.0%
! TGTGTATATATCATGTTTCA+TGG - chr1_1:64715375-64715394 Msa0036250:intron 25.0%
!! TTTCATGGAAAACCAATTTT+GGG - chr1_1:64715390-64715409 Msa0036250:intron 25.0%
!!! TGATTCAAACTTTGAAACAA+AGG + chr1_1:64715480-64715499 None:intergenic 25.0%
!!! CAATTTTGAGTCTGAAAATA+AGG - chr1_1:64715587-64715606 Msa0036250:intron 25.0%
! TCTGAAAATAAGGATTATGA+AGG - chr1_1:64715597-64715616 Msa0036250:intron 25.0%
! ACATAACTTAACTTATACCT+AGG + chr1_1:64715655-64715674 None:intergenic 25.0%
! TAGGTATAAGTTAAGTTATG+TGG - chr1_1:64715654-64715673 Msa0036250:intron 25.0%
! AAAATATCATCCTAGATACA+AGG + chr1_1:64715696-64715715 None:intergenic 25.0%
! ATTATCTTTGAGCAATCAAT+TGG + chr1_1:64716049-64716068 None:intergenic 25.0%
!! TAAATATGTATGTATTGGTG+TGG - chr1_1:64716096-64716115 Msa0036250:intron 25.0%
!! AAATATGTATGTATTGGTGT+GGG - chr1_1:64716097-64716116 Msa0036250:intron 25.0%
! TTGTAGTTCATTGACATAAA+TGG - chr1_1:64716194-64716213 Msa0036250:intron 25.0%
! ATGTTTCAATAGAAACTACT+TGG - chr1_1:64716315-64716334 Msa0036250:intron 25.0%
!! GGAAGCATAAATAAATACTT+TGG - chr1_1:64716667-64716686 Msa0036250:intron 25.0%
! TAAAATTTCAAAAAGGTCCA+AGG - chr1_1:64716913-64716932 Msa0036250:intron 25.0%
! AAAATTTCAAAAAGGTCCAA+GGG - chr1_1:64716914-64716933 Msa0036250:intron 25.0%
! TTACATACATGAAAGAAAGA+TGG + chr1_1:64717013-64717032 None:intergenic 25.0%
! ATGTAGGAAGAAAAAGAATA+TGG - chr1_1:64717074-64717093 Msa0036250:intron 25.0%
! TGTAGGAAGAAAAAGAATAT+GGG - chr1_1:64717075-64717094 Msa0036250:intron 25.0%
! GATACAGTCTATATCTTAAA+TGG - chr1_1:64717342-64717361 Msa0036250:intron 25.0%
!!! ATAAACTTCCAATTTGTTGT+GGG - chr1_1:64717407-64717426 Msa0036250:intron 25.0%
!!! ATTTGCTATGTTAAGAACAA+TGG + chr1_1:64717498-64717517 None:intergenic 25.0%
!! TGAAGATAAGTTGAATCAAA+TGG + chr1_1:64717667-64717686 None:intergenic 25.0%
! AAATGTAAATGTAGGGATAT+TGG + chr1_1:64717874-64717893 None:intergenic 25.0%
!!! AACATTTTAATTTGCACCTT+TGG - chr1_1:64717905-64717924 Msa0036250:CDS 25.0%
! GATCTATTTGCAAAATCTTT+GGG + chr1_1:64717931-64717950 None:intergenic 25.0%
! AGATCTATTTGCAAAATCTT+TGG + chr1_1:64717932-64717951 None:intergenic 25.0%
TGTCTTTGATTCATTCTCAA+TGG + chr1_1:64714827-64714846 None:intergenic 30.0%
!! ACACAATTCTAAGGTTTCAT+GGG + chr1_1:64714871-64714890 None:intergenic 30.0%
ATGTTTGTGACACAATTCTA+AGG + chr1_1:64714880-64714899 None:intergenic 30.0%
TTAGAATTGTGTCACAAACA+TGG - chr1_1:64714879-64714898 Msa0036250:three_prime_UTR 30.0%
!! TTGAGATTTGGTGAATGTAA+CGG + chr1_1:64714920-64714939 None:intergenic 30.0%
!!! GTGTTTTTTGGTTTGAAAGA+GGG + chr1_1:64714949-64714968 None:intergenic 30.0%
!!! TGTGTTTTTTGGTTTGAAAG+AGG + chr1_1:64714950-64714969 None:intergenic 30.0%
!!! TGAATTTGGGTTGTGTTTTT+TGG + chr1_1:64714961-64714980 None:intergenic 30.0%
TGCTGAAATTCTAGTAAAAG+AGG + chr1_1:64715070-64715089 None:intergenic 30.0%
ATTAGGGAACAACAACATAT+CGG + chr1_1:64715121-64715140 None:intergenic 30.0%
! CAATGCTTTGAATTTCCATT+AGG + chr1_1:64715138-64715157 None:intergenic 30.0%
CTAATGGAAATTCAAAGCAT+TGG - chr1_1:64715136-64715155 Msa0036250:three_prime_UTR 30.0%
TGGTCAAAATTTGAGCATAA+TGG + chr1_1:64715218-64715237 None:intergenic 30.0%
! TACTTACTTTCCCATGATTT+TGG + chr1_1:64715238-64715257 None:intergenic 30.0%
AAATCATGGGAAAGTAAGTA+AGG - chr1_1:64715238-64715257 Msa0036250:three_prime_UTR 30.0%
!! GCTTTGAAATTTGATGACAA+TGG - chr1_1:64715322-64715341 Msa0036250:three_prime_UTR 30.0%
! GTTTCATGGAAAACCAATTT+TGG - chr1_1:64715389-64715408 Msa0036250:intron 30.0%
TTCACAACAAAACCCAAAAT+TGG + chr1_1:64715405-64715424 None:intergenic 30.0%
! TTATGTGGTATGTGTGATTT+TGG - chr1_1:64715669-64715688 Msa0036250:exon 30.0%
TTGATAGAAACTAGTTCAGA+AGG - chr1_1:64716218-64716237 Msa0036250:intron 30.0%
CATATGTTCAGGTAGAAATT+TGG - chr1_1:64716446-64716465 Msa0036250:intron 30.0%
! ACAAAGAAACTTTTTGTGAC+AGG - chr1_1:64716525-64716544 Msa0036250:intron 30.0%
!! ACGACATAACATTGATTCTA+AGG - chr1_1:64716555-64716574 Msa0036250:intron 30.0%
! ACTTTTATCTCCTATTCATC+TGG - chr1_1:64716594-64716613 Msa0036250:intron 30.0%
AGTTATTTGTCTCTTGAACA+GGG - chr1_1:64716724-64716743 Msa0036250:intron 30.0%
ATTCATTGTTCTCTTGTAGA+AGG - chr1_1:64716828-64716847 Msa0036250:intron 30.0%
TTCAATAAATGCATAACCCT+TGG + chr1_1:64716933-64716952 None:intergenic 30.0%
ATTTATTGAATACACCACAG+AGG - chr1_1:64716943-64716962 Msa0036250:intron 30.0%
! TTCTTTTCTTATCAAGCAGA+TGG + chr1_1:64717224-64717243 None:intergenic 30.0%
GATAAGAAAAGAAGTCTGTA+AGG - chr1_1:64717231-64717250 Msa0036250:intron 30.0%
!! CATAAACTTCCAATTTGTTG+TGG - chr1_1:64717406-64717425 Msa0036250:intron 30.0%
GAAATGATAGAGAAGTAACT+TGG + chr1_1:64717618-64717637 None:intergenic 30.0%
TAACTACTGAAAAACATCTC+CGG + chr1_1:64717726-64717745 None:intergenic 30.0%
!! GACACAATTCTAAGGTTTCA+TGG + chr1_1:64714872-64714891 None:intergenic 35.0%
!!! TTTTGGTTTGAAAGAGGGAA+GGG + chr1_1:64714944-64714963 None:intergenic 35.0%
!!! TTTTTGGTTTGAAAGAGGGA+AGG + chr1_1:64714945-64714964 None:intergenic 35.0%
!! AACAACAACATATCGGTTTG+TGG + chr1_1:64715114-64715133 None:intergenic 35.0%
! GATATGTTGTTGTTCCCTAA+TGG - chr1_1:64715120-64715139 Msa0036250:three_prime_UTR 35.0%
!! AAAGCATTGGGTTGTTAGAA+TGG - chr1_1:64715149-64715168 Msa0036250:three_prime_UTR 35.0%
ATGGTCAACAATTTGAGCTT+TGG + chr1_1:64715199-64715218 None:intergenic 35.0%
!! GTTTTGTTGTGAACTTGATG+AGG - chr1_1:64715412-64715431 Msa0036250:intron 35.0%
TCAGACTCAAAATTGTCATC+TGG + chr1_1:64715582-64715601 None:intergenic 35.0%
!! TGATTTTGGTCCTTGTATCT+AGG - chr1_1:64715683-64715702 Msa0036250:CDS 35.0%
GAAGTAGTGATGATCAATGA+AGG - chr1_1:64715924-64715943 Msa0036250:CDS 35.0%
!! TGACTTGCTTCAGAAGAATT+TGG + chr1_1:64716489-64716508 None:intergenic 35.0%
!! AAATTCTTCTGAAGCAAGTC+AGG - chr1_1:64716488-64716507 Msa0036250:intron 35.0%
GTAAGAACATCCAGATGAAT+AGG + chr1_1:64716607-64716626 None:intergenic 35.0%
TGTGTGAATTACTCTCTCAA+TGG - chr1_1:64716646-64716665 Msa0036250:intron 35.0%
CAGTTATTTGTCTCTTGAAC+AGG - chr1_1:64716723-64716742 Msa0036250:intron 35.0%
TTGTGCGTGCAGATTATTAA+TGG - chr1_1:64717134-64717153 Msa0036250:intron 35.0%
TATTAATGGCTGGATGATAG+TGG - chr1_1:64717148-64717167 Msa0036250:intron 35.0%
AATGCATGCTTCATTCAACA+AGG - chr1_1:64717550-64717569 Msa0036250:intron 35.0%
CTTGGACGATGATGTTATAA+GGG - chr1_1:64717573-64717592 Msa0036250:intron 35.0%
TTGGACGATGATGTTATAAG+GGG - chr1_1:64717574-64717593 Msa0036250:intron 35.0%
ATAGAGAAGTAACTTGGTCA+GGG + chr1_1:64717612-64717631 None:intergenic 35.0%
TTGAATCAAATGGAGACAGA+GGG + chr1_1:64717657-64717676 None:intergenic 35.0%
TAGACACCCATAATATTCTC+CGG - chr1_1:64717704-64717723 Msa0036250:CDS 35.0%
ACATCTCCGGAGAATATTAT+GGG + chr1_1:64717713-64717732 None:intergenic 35.0%
AACATCTCCGGAGAATATTA+TGG + chr1_1:64717714-64717733 None:intergenic 35.0%
TTATAACCACACATGCTCTT+AGG - chr1_1:64717750-64717769 Msa0036250:CDS 35.0%
CCATTTCATAAAGATGTCAG+AGG + chr1_1:64717961-64717980 None:intergenic 35.0%
CCTCTGACATCTTTATGAAA+TGG - chr1_1:64717958-64717977 Msa0036250:CDS 35.0%
CGGTTTGTGGAATTGATGTT+TGG + chr1_1:64715101-64715120 None:intergenic 40.0%
AGAATGGATAAACCTGCTGT+TGG - chr1_1:64715165-64715184 Msa0036250:three_prime_UTR 40.0%
TGAACTTGTGATTGTGTTGC+AGG - chr1_1:64715531-64715550 Msa0036250:intron 40.0%
AGACACTTGTCTAACACATG+TGG + chr1_1:64715809-64715828 None:intergenic 40.0%
!! TCATAGTCATAGAAGTGTCG+GGG + chr1_1:64715888-64715907 None:intergenic 40.0%
!! CTCATAGTCATAGAAGTGTC+GGG + chr1_1:64715889-64715908 None:intergenic 40.0%
! CCTCATAGTCATAGAAGTGT+CGG + chr1_1:64715890-64715909 None:intergenic 40.0%
CCTGAACATATGTAGTTAGC+AGG + chr1_1:64716438-64716457 None:intergenic 40.0%
CCTGCTAACTACATATGTTC+AGG - chr1_1:64716435-64716454 Msa0036250:CDS 40.0%
! GGAGAACATTTTTGCTTGCA+GGG - chr1_1:64716689-64716708 Msa0036250:intron 40.0%
TATTGAATACACCACAGAGG+AGG - chr1_1:64716946-64716965 Msa0036250:intron 40.0%
ACTCAAAGAGATGAATGGCA+AGG - chr1_1:64716982-64717001 Msa0036250:intron 40.0%
! AATGCAATTTTGCTTTGCGC+TGG - chr1_1:64717033-64717052 Msa0036250:intron 40.0%
TTCTTATCAAGCAGATGGTC+TGG + chr1_1:64717219-64717238 None:intergenic 40.0%
CGAAGACTGAATTCATTCCA+CGG + chr1_1:64717279-64717298 None:intergenic 40.0%
! TTGCATTTAGCTCCCAAATC+TGG + chr1_1:64717532-64717551 None:intergenic 40.0%
ATGCTTCATTCAACAAGGCT+TGG - chr1_1:64717555-64717574 Msa0036250:intron 40.0%
GCTTGGACGATGATGTTATA+AGG - chr1_1:64717572-64717591 Msa0036250:intron 40.0%
TAGAGAAGTAACTTGGTCAG+GGG + chr1_1:64717611-64717630 None:intergenic 40.0%
GATAGAGAAGTAACTTGGTC+AGG + chr1_1:64717613-64717632 None:intergenic 40.0%
TGAATCAAATGGAGACAGAG+GGG + chr1_1:64717656-64717675 None:intergenic 40.0%
GTTGAATCAAATGGAGACAG+AGG + chr1_1:64717658-64717677 None:intergenic 40.0%
GCGAATCTAGTGTATCATGA+GGG - chr1_1:64717812-64717831 Msa0036250:CDS 40.0%
TTTGAAAGTTCAGTGTGTGC+AGG + chr1_1:64717844-64717863 None:intergenic 40.0%
TTGCAAAATCTTTGGGCCAA+AGG + chr1_1:64717924-64717943 None:intergenic 40.0%
! CACATACTTTTCTGTCCAAC+AGG - chr1_1:64717988-64718007 Msa0036250:CDS 40.0%
! AGAGGGAAGGGTTTGAGATT+TGG + chr1_1:64714932-64714951 None:intergenic 45.0%
! TTGGTAACAACACCAACAGC+AGG + chr1_1:64715180-64715199 None:intergenic 45.0%
GGTGTTTGTTTGCAGTGAGA+AGG - chr1_1:64715343-64715362 Msa0036250:three_prime_UTR 45.0%
ATTAAGCATGTCCTGGTGAC+CGG - chr1_1:64715758-64715777 Msa0036250:intron 45.0%
TTGTCTAACACATGTGGCAC+GGG + chr1_1:64715803-64715822 None:intergenic 45.0%
CTTGTCTAACACATGTGGCA+CGG + chr1_1:64715804-64715823 None:intergenic 45.0%
CCGACACTTCTATGACTATG+AGG - chr1_1:64715887-64715906 Msa0036250:CDS 45.0%
ACGCGGACACGTTGAAATAA+AGG + chr1_1:64716126-64716145 None:intergenic 45.0%
ATGAAGCACAGACTTTGACG+CGG + chr1_1:64716149-64716168 None:intergenic 45.0%
! GGGAGAACATTTTTGCTTGC+AGG - chr1_1:64716688-64716707 Msa0036250:intron 45.0%
CTCACCAAAGCCTTCAAATG+CGG + chr1_1:64716784-64716803 None:intergenic 45.0%
GCAGCACTCAAAGAGATGAA+TGG - chr1_1:64716977-64716996 Msa0036250:intron 45.0%
CTGCTTCGTGATTGTCATGT+AGG - chr1_1:64717058-64717077 Msa0036250:intron 45.0%
GCGTGCAGATTATTAATGGC+TGG - chr1_1:64717138-64717157 Msa0036250:intron 45.0%
ACCCCACCAAGATACAACAA+GGG - chr1_1:64717188-64717207 Msa0036250:intron 45.0%
! ACCCTTGTTGTATCTTGGTG+GGG + chr1_1:64717192-64717211 None:intergenic 45.0%
! GACCCTTGTTGTATCTTGGT+GGG + chr1_1:64717193-64717212 None:intergenic 45.0%
GCTATTAATTACCAGCTCCG+TGG - chr1_1:64717259-64717278 Msa0036250:intron 45.0%
!! CTTCCAATTTGTTGTGGGTG+TGG - chr1_1:64717412-64717431 Msa0036250:intron 45.0%
TGTCCACACCCACAACAAAT+TGG + chr1_1:64717418-64717437 None:intergenic 45.0%
ACAATGGCATGAGTGAACAC+TGG + chr1_1:64717482-64717501 None:intergenic 45.0%
! TCACTTCCTAAGAGCATGTG+TGG + chr1_1:64717759-64717778 None:intergenic 45.0%
GGCGAATCTAGTGTATCATG+AGG - chr1_1:64717811-64717830 Msa0036250:CDS 45.0%
!!! TTTTTTTTTTTCTTTTTTAT+TGG - chr1_1:64715288-64715307 Msa0036250:three_prime_UTR 5.0%
!!! TTTTTTTTTTCTTTTTTATT+GGG - chr1_1:64715289-64715308 Msa0036250:three_prime_UTR 5.0%
!!! AAAATACTAAAATTTAATTT+TGG - chr1_1:64718086-64718105 Msa0036250:five_prime_UTR 5.0%
GATTGTGCACATGAGCTAGC+TGG - chr1_1:64715434-64715453 Msa0036250:intron 50.0%
! ATGCTTAATGCCAGAGGCGT+CGG + chr1_1:64715747-64715766 None:intergenic 50.0%
CAGGACATGCTTAATGCCAG+AGG + chr1_1:64715753-64715772 None:intergenic 50.0%
CTCTGGCATTAAGCATGTCC+TGG - chr1_1:64715751-64715770 Msa0036250:intron 50.0%
!! AAGTGTCGGGGATGTGTCTA+AGG + chr1_1:64715876-64715895 None:intergenic 50.0%
!! GGCTTTGGTGAGCTTGTTGA+AGG - chr1_1:64716792-64716811 Msa0036250:intron 50.0%
! CTCTTTGAGTGCTGCACTTG+CGG + chr1_1:64716972-64716991 None:intergenic 50.0%
! CTTGTTGTATCTTGGTGGGG+TGG + chr1_1:64717189-64717208 None:intergenic 50.0%
CACCCCACCAAGATACAACA+AGG - chr1_1:64717187-64717206 Msa0036250:intron 50.0%
! CGACCCTTGTTGTATCTTGG+TGG + chr1_1:64717194-64717213 None:intergenic 50.0%
! GCACGACCCTTGTTGTATCT+TGG + chr1_1:64717197-64717216 None:intergenic 50.0%
CTGAATTCATTCCACGGAGC+TGG + chr1_1:64717273-64717292 None:intergenic 50.0%
TCAAATGGAGACAGAGGGGA+AGG + chr1_1:64717652-64717671 None:intergenic 50.0%
! TCTGTCCAACAGGCTTGTTC+TGG - chr1_1:64717998-64718017 Msa0036250:exon 50.0%
! TCCAACAGGCTTGTTCTGGT+AGG - chr1_1:64718002-64718021 Msa0036250:exon 50.0%
GATTGTGTTGCAGGTGTGCC+TGG - chr1_1:64715540-64715559 Msa0036250:intron 55.0%
GGCTGAACAGACAAGACACC+AGG + chr1_1:64715561-64715580 None:intergenic 55.0%
AACACATGTGGCACGGGTGT+CGG + chr1_1:64715797-64715816 None:intergenic 55.0%
ACCTTACGCGCCGCATTTGA+AGG - chr1_1:64716771-64716790 Msa0036250:intron 55.0%
GTATCTTGGTGGGGTGGTCT+TGG + chr1_1:64717183-64717202 None:intergenic 55.0%
CACGTACCAGCGATCACTAG+AGG - chr1_1:64717790-64717809 Msa0036250:CDS 55.0%
GATTCGCCTCTAGTGATCGC+TGG + chr1_1:64717799-64717818 None:intergenic 55.0%
GCCTACCAGAACAAGCCTGT+TGG + chr1_1:64718006-64718025 None:intergenic 55.0%
TCCTGGTGACCGGCACACAT+CGG - chr1_1:64715768-64715787 Msa0036250:intron 60.0%
TGTCGGACACCGATGTGTGC+CGG + chr1_1:64715780-64715799 None:intergenic 60.0%
CACAGACTTTGACGCGGACG+CGG + chr1_1:64716143-64716162 None:intergenic 60.0%
GCCTTCAAATGCGGCGCGTA+AGG + chr1_1:64716775-64716794 None:intergenic 60.0%
CGCGCCGCATTTGAAGGCTT+TGG - chr1_1:64716777-64716796 Msa0036250:intron 60.0%
TTGGTCAGCTCCGACGCCTC+TGG - chr1_1:64715734-64715753 Msa0036250:CDS 65.0%
! ACCGATGTGTGCCGGTCACC+AGG + chr1_1:64715772-64715791 None:intergenic 65.0%
TGCACTTGCGGCCTCCTCTG+TGG + chr1_1:64716960-64716979 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 64714789 64718122 64714789 ID=Msa0036250;Name=Msa0036250
chr1_1 mRNA 64714789 64718122 64714789 ID=Msa0036250-mRNA-1;Parent=Msa0036250;Name=Msa0036250-mRNA-1;_AED=0.02;_eAED=0.02;_QI=109|0.85|0.87|1|0.85|0.75|8|610|296
chr1_1 exon 64714789 64715378 64714789 ID=Msa0036250-mRNA-1:exon:20770;Parent=Msa0036250-mRNA-1
chr1_1 exon 64715659 64715765 64715659 ID=Msa0036250-mRNA-1:exon:20769;Parent=Msa0036250-mRNA-1
chr1_1 exon 64715887 64716014 64715887 ID=Msa0036250-mRNA-1:exon:20768;Parent=Msa0036250-mRNA-1
chr1_1 exon 64716098 64716166 64716098 ID=Msa0036250-mRNA-1:exon:20767;Parent=Msa0036250-mRNA-1
chr1_1 exon 64716365 64716454 64716365 ID=Msa0036250-mRNA-1:exon:20766;Parent=Msa0036250-mRNA-1
chr1_1 exon 64717293 64717358 64717293 ID=Msa0036250-mRNA-1:exon:20765;Parent=Msa0036250-mRNA-1
chr1_1 exon 64717456 64717553 64717456 ID=Msa0036250-mRNA-1:exon:20764;Parent=Msa0036250-mRNA-1
chr1_1 exon 64717661 64718122 64717661 ID=Msa0036250-mRNA-1:exon:20763;Parent=Msa0036250-mRNA-1
chr1_1 five_prime_UTR 64718014 64718122 64718014 ID=Msa0036250-mRNA-1:five_prime_utr;Parent=Msa0036250-mRNA-1
chr1_1 CDS 64717661 64718013 64717661 ID=Msa0036250-mRNA-1:cds;Parent=Msa0036250-mRNA-1
chr1_1 CDS 64717456 64717553 64717456 ID=Msa0036250-mRNA-1:cds;Parent=Msa0036250-mRNA-1
chr1_1 CDS 64717293 64717358 64717293 ID=Msa0036250-mRNA-1:cds;Parent=Msa0036250-mRNA-1
chr1_1 CDS 64716365 64716454 64716365 ID=Msa0036250-mRNA-1:cds;Parent=Msa0036250-mRNA-1
chr1_1 CDS 64716098 64716166 64716098 ID=Msa0036250-mRNA-1:cds;Parent=Msa0036250-mRNA-1
chr1_1 CDS 64715887 64716014 64715887 ID=Msa0036250-mRNA-1:cds;Parent=Msa0036250-mRNA-1
chr1_1 CDS 64715679 64715765 64715679 ID=Msa0036250-mRNA-1:cds;Parent=Msa0036250-mRNA-1
chr1_1 three_prime_UTR 64715659 64715678 64715659 ID=Msa0036250-mRNA-1:three_prime_utr;Parent=Msa0036250-mRNA-1
chr1_1 three_prime_UTR 64714789 64715378 64714789 ID=Msa0036250-mRNA-1:three_prime_utr;Parent=Msa0036250-mRNA-1
Gene Sequence

>Msa0036250

ATGGAAGTTCTGTTTCTCTCCGTTACATTCACCAAATCTCAAACCCTTCCCTCTTTCAAACCAAAAAACACAACCCAAATTCACCAACTTCAAATAAAAACAAAACTACCCAACAAAAACAATCACTCTAATAATCTTCTCTCTTCTTCTTGTTCCATTTCATGTAATTCCTCTTTTACTAGAATTTCAGCAACAAGTAACCAAACATCAATTCCACAAACCGATATGTTGTTGTTCCCTAATGGAAATTCAAAGCATTGGGTTGTTAGAATGGATAAACCTGCTGTTGGTGTTGTTACCAAAGCTCAAATTGTTGACCATTATGCTCAAATTTTGACCAAAATCATGGGAAATGAGAAGGATGCACAAATGTGTATATATCATGTTTCATGGAAAACCAATTTTGGGTTTTGTTGTGAACTTGATGAGGATTGTGCACATGAGCTAGCTGGTGTGCCTGGTGTCTTGTCTGTTCAGCCAGATGACAATTTTGAGTCTGAAAATAAGGATTATGAAGGTAGAAATTTGGAAAATAGTCTGAATATGCCAAATTCTTCTGAAGCAAGTCAGGAAGCTTCTGTGAAAACAAAGAAACTTTTTGTGACAGGGCTATCATTTTATACATCTGAGAAAACCTTACGCGCCGCATTTGAAGGCTTTGGTGAGCTTGTTGAAGTTAAAGTTATTATAGATAAAATTTCAAAAAGGTCCAAGGGTTATGCATTTATTGAATACACCACAGAGGAGGCCGCAAGTGCAGCACTCAAAGAGATGAATGGCAAGGTAGATCCATCTTTCTTTCATATTATTAATGGCTGGATGATAGTGGTAGATGTTGCCAAGACCACCCCACCAAGATACAACAAGGGTCGTGCCAGACCATCTGCTTGA
Protein sequence

>Msa0036250

MEVLFLSVTFTKSQTLPSFKPKNTTQIHQLQIKTKLPNKNNHSNNLLSSSCSISCNSSFTRISATSNQTSIPQTDMLLFPNGNSKHWVVRMDKPAVGVVTKAQIVDHYAQILTKIMGNEKDAQMCIYHVSWKTNFGFCCELDEDCAHELAGVPGVLSVQPDDNFESENKDYEGRNLENSLNMPNSSEASQEASVKTKKLFVTGLSFYTSEKTLRAAFEGFGELVEVKVIIDKISKRSKGYAFIEYTTEEAASAALKEMNGKVDPSFFHIINGWMIVVDVAKTTPPRYNKGRARPSA*