AlfalfaGEDB | Msa1277640

M. sativa cultivar zhongmu-4 / Msa1277640

KEGG: K07904
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005794 GO:0008150 GO:0009504 GO:0009987 GO:0012505 GO:0031410 GO:0031982 GO:0042546 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0071554 GO:0071840 GO:0097708
PFAM: PF00071.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1277640	sp\|Q9LNK1\|RABA3_ARATH	73.077	234	59	3	2	232	5	237	2.72e-117	337

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1277640	G7LAV3	99.138	232	2	0	1	232	1	232	1.62e-166	468

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003080	Msa1277640	0.834770	2.473766e-56	-8.615850e-47
Msa0005890	Msa1277640	0.806829	6.801114e-50	-8.615850e-47
Msa0017670	Msa1277640	0.817622	2.998090e-52	-8.615850e-47
Msa0019900	Msa1277640	0.850036	2.201580e-60	-8.615850e-47
Msa0025160	Msa1277640	0.823099	1.661729e-53	-8.615850e-47
Msa0025580	Msa1277640	0.810039	1.404977e-50	-8.615850e-47
Msa0050660	Msa1277640	0.866219	3.345801e-65	-8.615850e-47
Msa0050700	Msa1277640	0.866902	2.030125e-65	-8.615850e-47
Msa0050790	Msa1277640	0.878895	1.939516e-69	-8.615850e-47
Msa0054690	Msa1277640	0.815446	9.206457e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1277640	MtrunA17_Chr8g0346421	99.138	232	2	0	1	232	1	232	3.13e-170	468
Msa1277640	MtrunA17_Chr7g0275991	82.243	214	36	1	21	232	15	228	1.32e-128	362
Msa1277640	MtrunA17_Chr8g0381841	66.507	209	65	2	18	222	9	216	4.27e-96	280
Msa1277640	MtrunA17_Chr3g0123821	64.516	217	69	2	18	230	8	220	3.63e-95	277
Msa1277640	MtrunA17_Chr1g0174281	66.337	202	61	2	18	213	7	207	1.16e-92	271
Msa1277640	MtrunA17_Chr7g0231131	66.000	200	64	1	18	213	7	206	2.22e-90	265
Msa1277640	MtrunA17_Chr2g0283131	57.870	216	89	1	17	232	4	217	1.12e-86	256
Msa1277640	MtrunA17_Chr4g0046261	56.944	216	91	1	17	232	4	217	4.82e-86	254
Msa1277640	MtrunA17_Chr2g0276941	61.194	201	76	1	20	218	9	209	4.95e-85	251
Msa1277640	MtrunA17_Chr4g0040781	55.556	216	94	1	17	232	4	217	3.00e-84	249

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1277640	AT1G01200.1	73.077	234	59	3	2	232	5	237	2.76e-118	337
Msa1277640	AT5G65270.1	65.534	206	63	2	18	217	9	212	5.58e-96	280
Msa1277640	AT5G47960.1	66.981	212	64	3	14	220	3	213	2.98e-94	275
Msa1277640	AT3G12160.1	66.184	207	62	2	18	218	7	211	3.59e-94	275
Msa1277640	AT4G39990.1	63.679	212	70	4	22	230	13	220	3.23e-91	268
Msa1277640	AT4G18800.1	58.333	216	83	2	17	231	4	213	6.09e-86	254
Msa1277640	AT5G60860.1	56.279	215	92	1	17	231	4	216	2.91e-85	252
Msa1277640	AT3G15060.1	55.814	215	93	1	17	231	4	216	4.05e-84	249
Msa1277640	AT2G33870.1	55.300	217	94	2	17	232	4	218	6.35e-84	249
Msa1277640	AT1G16920.1	56.944	216	88	2	17	231	4	215	8.52e-84	249

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Find 69 sgRNAs with CRISPR-Local

Find 280 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAATAGGCAAGGAAGT+GGG	0.556156	8_3:+13374706	None:intergenic
AAAGAATAGGCAAGGAAGTG+GGG	0.651506	8_3:+13374707	None:intergenic
AAATGTTGGTCTTACCTTAG+GGG	0.622705	8_3:+13374597	None:intergenic
AACTCAACTGCATCTTCAGT+AGG	0.619231	8_3:+13372889	None:intergenic
AAGACCAGATGAAATCAATT+AGG	0.398197	8_3:+13372532	None:intergenic
AAGTAATTAAAGCTCAGATC+TGG	0.376078	8_3:-13374262	Msa1277640:CDS
AATTTGGTATGACTAAATGT+TGG	0.589533	8_3:+13374583	None:intergenic
ACTAGACTCTTATAAAACCT+TGG	0.513126	8_3:+13374529	None:intergenic
ACTAGGACTGTCACTATTAA+TGG	0.266282	8_3:-13374287	Msa1277640:CDS
AGCTCTTACTGGTGTGAATG+TGG	0.537237	8_3:-13372830	Msa1277640:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTTAACATGTTGTGAAACCA+AGG	-	chr8_3:13372657-13372676	Msa1277640:exon	30.0%
ACTAGACTCTTATAAAACCT+TGG	+	chr8_3:13372684-13372703	None:intergenic	30.0%
TCTTGTCTTGTGAAGAAAAA+TGG	-	chr8_3:13372712-13372731	Msa1277640:CDS	30.0%
TTTGACTCAAAGTCAACTAT+TGG	-	chr8_3:13372887-13372906	Msa1277640:CDS	30.0%
AAGTAATTAAAGCTCAGATC+TGG	-	chr8_3:13372948-13372967	Msa1277640:CDS	30.0%
AGTAATTAAAGCTCAGATCT+GGG	-	chr8_3:13372949-13372968	Msa1277640:CDS	30.0%
TGAAACTCAATGTAGTTCAA+GGG	+	chr8_3:13373067-13373086	None:intergenic	30.0%
TTGAAACTCAATGTAGTTCA+AGG	+	chr8_3:13373068-13373087	None:intergenic	30.0%
TTGCAATATTAGAGTGGTAT+TGG	-	chr8_3:13373205-13373224	Msa1277640:intron	30.0%
ATTAAAAGGAGAGAATACAC+CGG	-	chr8_3:13373598-13373617	Msa1277640:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	13372480	13374752	13372480	ID=Msa1277640;Name=Msa1277640
chr8_3	mRNA	13372480	13374752	13372480	ID=Msa1277640-mRNA-1;Parent=Msa1277640;Name=Msa1277640-mRNA-1;_AED=0.15;_eAED=0.15;_QI=251\|1\|1\|1\|1\|1\|2\|180\|232
chr8_3	exon	13372480	13373098	13372480	ID=Msa1277640-mRNA-1:exon:5240;Parent=Msa1277640-mRNA-1
chr8_3	exon	13374242	13374752	13374242	ID=Msa1277640-mRNA-1:exon:5239;Parent=Msa1277640-mRNA-1
chr8_3	five_prime_UTR	13374502	13374752	13374502	ID=Msa1277640-mRNA-1:five_prime_utr;Parent=Msa1277640-mRNA-1
chr8_3	CDS	13374242	13374501	13374242	ID=Msa1277640-mRNA-1:cds;Parent=Msa1277640-mRNA-1
chr8_3	CDS	13372660	13373098	13372660	ID=Msa1277640-mRNA-1:cds;Parent=Msa1277640-mRNA-1
chr8_3	three_prime_UTR	13372480	13372659	13372480	ID=Msa1277640-mRNA-1:three_prime_utr;Parent=Msa1277640-mRNA-1

Gene Sequence

>Msa1277640

ATGGTCCAGGGTGAGAAGAAGCAAGAGAATGGACACAAGAAAATAGATTATGATATGGAAGAGAAAATAGATTATGTGTTTAAGGTAGTTGTCATTGGTGACTCAGCAGTAGGAAAAACTCAAATACTATCAAGGTTTTCAAAGAATGAGTTTTGCTTTGACTCAAAGTCAACTATTGGAGTTGAATTCCAAACTAGGACTGTCACTATTAATGGTAAAGTAATTAAAGCTCAGATCTGGGATACTGCTGGCCAAGAAAGGTACAGGGCTGTAACAAGTGCATACTATAGAGGTGCATTAGGGGCAATGCTAGTATATGACATAACCAAAAGACAATCTTTTGACCATGTTGCTAAATGGGTTGAGGAACTTAGAGCACATGCAGATAACTCAAATGTGATCATGCTAGTTGGTAACAAAGGTGACCTTGTTGATTTGAGAATGGTTCCTACTGAAGATGCAGTTGAGTTTGCAGAAGACCAAGGTTTATTCTTCTCAGAGACTTCAGCTCTTACTGGTGTGAATGTGGAGGGTGCATTTATCAAATTGCTAGAAGAGATTAATAGTGTAGTTTCTAAAAAGGCATTGGAATGTGGTAATGTGAAGGGGATTGCACTAAAAGGGTCTAAGATTGATGTAATTTCAGGTGCTGAATTGGAAATTAGTGAGATAAAGAAGCTGTCTTCATGCTCTTGTTGA

Protein sequence

>Msa1277640

MVQGEKKQENGHKKIDYDMEEKIDYVFKVVVIGDSAVGKTQILSRFSKNEFCFDSKSTIGVEFQTRTVTINGKVIKAQIWDTAGQERYRAVTSAYYRGALGAMLVYDITKRQSFDHVAKWVEELRAHADNSNVIMLVGNKGDLVDLRMVPTEDAVEFAEDQGLFFSETSALTGVNVEGAFIKLLEEINSVVSKKALECGNVKGIALKGSKIDVISGAELEISEIKKLSSCSC*