AlfalfaGEDB | Msa1319290

M. sativa cultivar zhongmu-4 / Msa1319290

KEGG: K15095
GO: -
PFAM: PF00106.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1319290	XP_003627593.1	97.611	293	7	0	1	293	1	293	0.0	587

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1319290	sp\|Q9M2E2\|SDR1_ARATH	54.730	296	131	3	1	293	1	296	1.21e-113	332

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1319290	G7LBT1	97.611	293	7	0	1	293	1	293	0.0	587

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0014590	Msa1319290	0.817548	3.114765e-52	-8.615850e-47
Msa0024280	Msa1319290	0.803477	3.420934e-49	-8.615850e-47
Msa0048950	Msa1319290	0.821169	4.656465e-53	-8.615850e-47
Msa0059880	Msa1319290	0.827604	1.427016e-54	-8.615850e-47
Msa0069480	Msa1319290	0.805908	1.063677e-49	-8.615850e-47
Msa0072030	Msa1319290	0.813786	2.145535e-51	-8.615850e-47
Msa0074620	Msa1319290	0.834559	2.794842e-56	-8.615850e-47
Msa0084150	Msa1319290	0.809378	1.949413e-50	-8.615850e-47
Msa0120050	Msa1319290	0.805754	1.145711e-49	-8.615850e-47
Msa0147670	Msa1319290	0.800319	1.523759e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1319290	MtrunA17_Chr8g0349721	97.611	293	7	0	1	293	1	293	0.0	587
Msa1319290	MtrunA17_Chr8g0349701	96.587	293	10	0	1	293	1	293	0.0	581
Msa1319290	MtrunA17_Chr7g0247041	66.782	289	94	2	7	293	6	294	1.04e-134	383
Msa1319290	MtrunA17_Chr8g0349801	55.705	298	127	2	1	293	1	298	2.18e-119	344
Msa1319290	MtrunA17_Chr8g0349811	55.034	298	129	2	1	293	1	298	6.10e-119	343
Msa1319290	MtrunA17_Chr8g0349851	54.515	299	130	2	1	293	1	299	1.82e-118	342
Msa1319290	MtrunA17_Chr8g0349781	55.034	298	129	2	1	293	1	298	2.61e-118	342
Msa1319290	MtrunA17_Chr7g0247011	56.314	293	121	3	1	293	1	286	3.24e-117	338
Msa1319290	MtrunA17_Chr8g0349641	55.593	295	124	3	1	289	1	294	6.60e-113	328
Msa1319290	MtrunA17_Chr8g0349831	54.882	297	128	3	3	293	4	300	8.89e-113	327

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1319290	AT3G61220.1	54.730	296	131	3	1	293	1	296	1.23e-114	332
Msa1319290	AT3G61220.2	55.172	290	127	3	7	293	14	303	7.37e-113	328
Msa1319290	AT3G61220.3	55.172	290	127	3	7	293	38	327	2.10e-112	328
Msa1319290	AT2G24190.1	52.703	296	137	3	1	293	1	296	5.41e-109	318
Msa1319290	AT2G24190.2	52.759	290	134	3	7	293	12	301	5.87e-107	313
Msa1319290	AT1G01800.1	53.147	286	130	3	4	285	2	287	2.69e-103	303
Msa1319290	AT1G01800.2	51.010	198	95	1	90	285	55	252	9.15e-66	207
Msa1319290	AT5G51030.1	35.333	300	162	5	5	293	36	314	7.37e-58	188
Msa1319290	AT3G59710.1	37.589	282	151	7	9	286	31	291	3.14e-55	181
Msa1319290	AT3G59710.3	37.589	282	151	7	9	286	21	281	3.78e-55	181

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Find 55 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAATGCTGGTGTTGCTGG+AGG	0.655043	8_4:-22226949	Msa1319290:CDS
AATTGGCCAACATTTGTTTC+TGG	0.189389	8_4:-22225382	Msa1319290:CDS
ACACAATTTATGCGGAAACG+AGG	0.622206	8_4:+22225301	None:intergenic
ACAGGAGCTAATAAGGGAAT+TGG	0.408672	8_4:-22227262	Msa1319290:CDS
ACTAAAGAAGAAACACTTGT+AGG	0.407989	8_4:+22227091	None:intergenic
ACTGATATTAACGAGATGAA+AGG	0.577779	8_4:-22225259	Msa1319290:CDS
ACTGCTAGAAATGAGAAAAG+AGG	0.505341	8_4:-22227190	Msa1319290:CDS
ACTTCTCTTGCCCACTCATT+TGG	0.338248	8_4:+22225499	None:intergenic
ACTTGTAACAGGAGCTAATA+AGG	0.275992	8_4:-22227269	Msa1319290:CDS
AGACCAGAAGGGCCATCATC+TGG	0.442885	8_4:+22225184	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAAAAAAAGGCAAAGGAAA+AGG	+	chr8_4:22225178-22225197	None:intergenic	30.0%
ATGTGAAGAAAAAAAGGCAA+AGG	+	chr8_4:22225184-22225203	None:intergenic	30.0%
ACCTAGAAACAAGATTAACA+AGG	+	chr8_4:22225257-22225276	None:intergenic	30.0%
CTAATAAGGGAATTGGTTAT+GGG	-	chr8_4:22225295-22225314	Msa1319290:CDS	30.0%
TTATGGGATATGCAAGAAAT+TGG	-	chr8_4:22225311-22225330	Msa1319290:CDS	30.0%
GTAGAAAGTTTGAAAGAGTT+AGG	-	chr8_4:22225393-22225412	Msa1319290:CDS	30.0%
CTAAAGAAGAAACACTTGTA+GGG	+	chr8_4:22225461-22225480	None:intergenic	30.0%
ACTAAAGAAGAAACACTTGT+AGG	+	chr8_4:22225462-22225481	None:intergenic	30.0%
GAGTTCATCAAAATCCAATT+TGG	-	chr8_4:22225483-22225502	Msa1319290:CDS	30.0%
TGGAGAGAATGTTGTTAAAA+AGG	-	chr8_4:22225633-22225652	Msa1319290:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	22225147	22227425	22225147	ID=Msa1319290;Name=Msa1319290
chr8_4	mRNA	22225147	22227425	22225147	ID=Msa1319290-mRNA-1;Parent=Msa1319290;Name=Msa1319290-mRNA-1;_AED=0.33;_eAED=0.33;_QI=0\|0\|0\|1\|1\|1\|5\|0\|294
chr8_4	exon	22227406	22227425	22227406	ID=Msa1319290-mRNA-1:exon:7887;Parent=Msa1319290-mRNA-1
chr8_4	exon	22227051	22227297	22227051	ID=Msa1319290-mRNA-1:exon:7886;Parent=Msa1319290-mRNA-1
chr8_4	exon	22226918	22226974	22226918	ID=Msa1319290-mRNA-1:exon:7885;Parent=Msa1319290-mRNA-1
chr8_4	exon	22226005	22226184	22226005	ID=Msa1319290-mRNA-1:exon:7884;Parent=Msa1319290-mRNA-1
chr8_4	exon	22225147	22225527	22225147	ID=Msa1319290-mRNA-1:exon:7883;Parent=Msa1319290-mRNA-1
chr8_4	CDS	22227406	22227425	22227406	ID=Msa1319290-mRNA-1:cds;Parent=Msa1319290-mRNA-1
chr8_4	CDS	22227051	22227297	22227051	ID=Msa1319290-mRNA-1:cds;Parent=Msa1319290-mRNA-1
chr8_4	CDS	22226918	22226974	22226918	ID=Msa1319290-mRNA-1:cds;Parent=Msa1319290-mRNA-1
chr8_4	CDS	22226005	22226184	22226005	ID=Msa1319290-mRNA-1:cds;Parent=Msa1319290-mRNA-1
chr8_4	CDS	22225147	22225527	22225147	ID=Msa1319290-mRNA-1:cds;Parent=Msa1319290-mRNA-1

Gene Sequence

>Msa1319290

ATGACAGAAGCATCAAAAAGGTATGCACTTGTAACAGGAGCTAATAAGGGAATTGGTTATGGGATATGCAAGAAATTGGCTTCAAGAGGTGTTATGGTAGTGCTTACTGCTAGAAATGAGAAAAGAGGTTTAGATGCTGTAGAAAGTTTGAAAGAGTTAGGTCTTTCTGACTTTGTGATTTTTCATCAACTTGATGTTACTGACCCTACAAGTGTTTCTTCTTTAGTTGAGTTCATCAAAATCCAATTTGGGAAGCTTGATATCTTGGTGAACAATGCTGGTGTTGCTGGAGGAATAGTGAATGGAGAGAATGTTGTTAAAAAGGTAAGGGGTGAAATATCAGATTGGAATTTAGCACTGCGTCAAACTTATGAATTAGCTGAAGAATGTGTGGAAATAAACTTCTTTGGTGCTGAAAGAGTAACAGAAGCTCTTATTCCCCTGCTCCAACTATCTACTTCACCCAGGATTGTCAATGTTTCCTCTAGACGTGGACAATTCAAGTTTATGCCAAATGAGTGGGCAAGAGAAGTGTTTGATGACATCAAAAACGTTACAAATGAAAAACTTGGTGAAGTGCTTAGAGAATTTTTGAAAGATTACAAAGAAGGAGCATTGGAAACAAAAAATTGGCCAACATTTGTTTCTGGTTACACAATGGCAAAAGCAGCTTTAAATTCTTACACAAGGTTGCTAGCTTTGAAGCTCCCTCGTTTCCGCATAAATTGTGTATGCCCTGACTTTGTTAAGACTGATATTAACGAGATGAAAGGGTTTTTAAGCATTGATGAAGGTGCTGAATGTCCTGTGAATCTAGCATTGTTGCCAGATGATGGCCCTTCTGGTCTATTTTTTTTACATGATGAAGTTATTTCATATGAATAA

Protein sequence

>Msa1319290

MTEASKRYALVTGANKGIGYGICKKLASRGVMVVLTARNEKRGLDAVESLKELGLSDFVIFHQLDVTDPTSVSSLVEFIKIQFGKLDILVNNAGVAGGIVNGENVVKKVRGEISDWNLALRQTYELAEECVEINFFGAERVTEALIPLLQLSTSPRIVNVSSRRGQFKFMPNEWAREVFDDIKNVTNEKLGEVLREFLKDYKEGALETKNWPTFVSGYTMAKAALNSYTRLLALKLPRFRINCVCPDFVKTDINEMKGFLSIDEGAECPVNLALLPDDGPSGLFFLHDEVISYE*