Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0084150 | sp|Q41160|LCB3_ROBPS | 63.910 | 133 | 45 | 2 | 3 | 135 | 111 | 240 | 1.78e-51 | 167 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0084150 | A0A072VPS3 | 71.631 | 141 | 38 | 1 | 1 | 141 | 112 | 250 | 1.57e-59 | 194 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0004580 | Msa0084150 | 0.801307 | 9.579008e-49 | -8.615850e-47 |
| Msa0010150 | Msa0084150 | 0.808562 | 2.913882e-50 | -8.615850e-47 |
| Msa0014590 | Msa0084150 | 0.801586 | 8.398036e-49 | -8.615850e-47 |
| Msa0027860 | Msa0084150 | 0.809448 | 1.882671e-50 | -8.615850e-47 |
| Msa0036230 | Msa0084150 | 0.856611 | 2.861735e-62 | -8.615850e-47 |
| Msa0048950 | Msa0084150 | 0.808087 | 3.679320e-50 | -8.615850e-47 |
| Msa0059880 | Msa0084150 | 0.813796 | 2.135100e-51 | -8.615850e-47 |
| Msa0074620 | Msa0084150 | 0.819686 | 1.019672e-52 | -8.615850e-47 |
| Msa0084150 | Msa0084160 | 0.876381 | 1.461248e-68 | -8.615850e-47 |
| Msa0084150 | Msa0150910 | 0.807408 | 5.128743e-50 | -8.615850e-47 |
| Msa0084150 | Msa0163340 | 0.811918 | 5.505634e-51 | -8.615850e-47 |
| Msa0084150 | Msa0170990 | 0.815547 | 8.744268e-52 | -8.615850e-47 |
| Msa0084150 | Msa0219210 | 0.817655 | 2.945780e-52 | -8.615850e-47 |
| Msa0084150 | Msa0269720 | 0.805859 | 1.089280e-49 | -8.615850e-47 |
| Msa0084150 | Msa0274250 | 0.801920 | 7.170395e-49 | -8.615850e-47 |
| Msa0084150 | Msa0298270 | 0.813888 | 2.037306e-51 | -8.615850e-47 |
| Msa0084150 | Msa0336630 | 0.820821 | 5.600908e-53 | -8.615850e-47 |
| Msa0084150 | Msa0354540 | 0.812913 | 3.336399e-51 | -8.615850e-47 |
| Msa0084150 | Msa0366510 | 0.816323 | 5.867476e-52 | -8.615850e-47 |
| Msa0084150 | Msa0374040 | 0.815850 | 7.483411e-52 | -8.615850e-47 |
| Msa0084150 | Msa0401420 | 0.803672 | 3.117305e-49 | -8.615850e-47 |
| Msa0084150 | Msa0414480 | 0.828052 | 1.113833e-54 | -8.615850e-47 |
| Msa0084150 | Msa0425950 | 0.802053 | 6.733790e-49 | -8.615850e-47 |
| Msa0084150 | Msa0491760 | 0.804144 | 2.486787e-49 | -8.615850e-47 |
| Msa0084150 | Msa0531030 | 0.809563 | 1.779114e-50 | -8.615850e-47 |
| Msa0084150 | Msa0531080 | 0.808627 | 2.822296e-50 | -8.615850e-47 |
| Msa0084150 | Msa0535570 | 0.835803 | 1.356418e-56 | -8.615850e-47 |
| Msa0084150 | Msa0543740 | 0.820669 | 6.069259e-53 | -8.615850e-47 |
| Msa0084150 | Msa0549170 | 0.809004 | 2.343631e-50 | -8.615850e-47 |
| Msa0084150 | Msa0549970 | 0.800002 | 1.768083e-48 | -8.615850e-47 |
| Msa0084150 | Msa0554660 | 0.807723 | 4.398096e-50 | -8.615850e-47 |
| Msa0084150 | Msa0555920 | 0.802562 | 5.288654e-49 | -8.615850e-47 |
| Msa0084150 | Msa0587920 | 0.822034 | 2.940051e-53 | -8.615850e-47 |
| Msa0084150 | Msa0589460 | 0.818298 | 2.108676e-52 | -8.615850e-47 |
| Msa0084150 | Msa0591490 | 0.835446 | 1.670683e-56 | -8.615850e-47 |
| Msa0084150 | Msa0599600 | 0.817517 | 3.164620e-52 | -8.615850e-47 |
| Msa0084150 | Msa0603950 | 0.808423 | 3.120394e-50 | -8.615850e-47 |
| Msa0084150 | Msa0604200 | 0.823520 | 1.325501e-53 | -8.615850e-47 |
| Msa0084150 | Msa0604280 | 0.800145 | 1.653286e-48 | -8.615850e-47 |
| Msa0084150 | Msa0605470 | 0.804065 | 2.582551e-49 | -8.615850e-47 |
| Msa0084150 | Msa0605480 | 0.813920 | 2.004429e-51 | -8.615850e-47 |
| Msa0084150 | Msa0629720 | 0.826568 | 2.525740e-54 | -8.615850e-47 |
| Msa0084150 | Msa0631700 | 0.824213 | 9.119045e-54 | -8.615850e-47 |
| Msa0084150 | Msa0640290 | 0.837528 | 4.929566e-57 | -8.615850e-47 |
| Msa0084150 | Msa0643840 | 0.839925 | 1.183273e-57 | -8.615850e-47 |
| Msa0084150 | Msa0646440 | 0.801778 | 7.669482e-49 | -8.615850e-47 |
| Msa0084150 | Msa0650310 | 0.805179 | 1.512217e-49 | -8.615850e-47 |
| Msa0084150 | Msa0667600 | 0.817256 | 3.623809e-52 | -8.615850e-47 |
| Msa0084150 | Msa0670360 | 0.844882 | 5.739996e-59 | -8.615850e-47 |
| Msa0084150 | Msa0678620 | 0.816985 | 4.169519e-52 | -8.615850e-47 |
| Msa0084150 | Msa0684720 | 0.818403 | 1.996345e-52 | -8.615850e-47 |
| Msa0084150 | Msa0685080 | 0.803333 | 3.665795e-49 | -8.615850e-47 |
| Msa0084150 | Msa0727480 | 0.807541 | 4.807567e-50 | -8.615850e-47 |
| Msa0084150 | Msa0768670 | 0.804546 | 2.051059e-49 | -8.615850e-47 |
| Msa0084150 | Msa0768720 | 0.801310 | 9.563926e-49 | -8.615850e-47 |
| Msa0084150 | Msa0814160 | 0.803796 | 2.938100e-49 | -8.615850e-47 |
| Msa0084150 | Msa0814230 | 0.832213 | 1.074561e-55 | -8.615850e-47 |
| Msa0084150 | Msa0824850 | 0.804416 | 2.183096e-49 | -8.615850e-47 |
| Msa0084150 | Msa0824860 | 0.820119 | 8.117959e-53 | -8.615850e-47 |
| Msa0084150 | Msa0863840 | 0.826900 | 2.104152e-54 | -8.615850e-47 |
| Msa0084150 | Msa0863850 | 0.808044 | 3.757039e-50 | -8.615850e-47 |
| Msa0084150 | Msa0864160 | 0.802093 | 6.605600e-49 | -8.615850e-47 |
| Msa0084150 | Msa0883640 | 0.806037 | 9.993438e-50 | -8.615850e-47 |
| Msa0084150 | Msa0893840 | 0.832930 | 7.135482e-56 | -8.615850e-47 |
| Msa0084150 | Msa0896920 | 0.811531 | 6.684111e-51 | -8.615850e-47 |
| Msa0084150 | Msa0910830 | 0.814129 | 1.803049e-51 | -8.615850e-47 |
| Msa0084150 | Msa0913550 | 0.828981 | 6.643345e-55 | -8.615850e-47 |
| Msa0084150 | Msa0946310 | 0.806598 | 7.610320e-50 | -8.615850e-47 |
| Msa0084150 | Msa0951150 | 0.831431 | 1.676340e-55 | -8.615850e-47 |
| Msa0084150 | Msa0960090 | 0.808692 | 2.733057e-50 | -8.615850e-47 |
| Msa0084150 | Msa0971880 | 0.828622 | 8.115930e-55 | -8.615850e-47 |
| Msa0084150 | Msa0985700 | 0.808932 | 2.429370e-50 | -8.615850e-47 |
| Msa0084150 | Msa0987640 | 0.809937 | 1.478161e-50 | -8.615850e-47 |
| Msa0084150 | Msa0996980 | 0.805010 | 1.640962e-49 | -8.615850e-47 |
| Msa0084150 | Msa1003110 | 0.817323 | 3.499879e-52 | -8.615850e-47 |
| Msa0084150 | Msa1021620 | 0.818270 | 2.140124e-52 | -8.615850e-47 |
| Msa0084150 | Msa1037350 | 0.816161 | 6.376847e-52 | -8.615850e-47 |
| Msa0084150 | Msa1061670 | 0.856411 | 3.275724e-62 | -8.615850e-47 |
| Msa0084150 | Msa1064940 | 0.800797 | 1.217909e-48 | -8.615850e-47 |
| Msa0084150 | Msa1076830 | 0.824893 | 6.307280e-54 | -8.615850e-47 |
| Msa0084150 | Msa1106680 | 0.811610 | 6.422504e-51 | -8.615850e-47 |
| Msa0084150 | Msa1106960 | 0.850736 | 1.400128e-60 | -8.615850e-47 |
| Msa0084150 | Msa1109880 | 0.817019 | 4.096745e-52 | -8.615850e-47 |
| Msa0084150 | Msa1109890 | 0.821248 | 4.466455e-53 | -8.615850e-47 |
| Msa0084150 | Msa1112730 | 0.806879 | 6.636876e-50 | -8.615850e-47 |
| Msa0084150 | Msa1123010 | 0.815426 | 9.300439e-52 | -8.615850e-47 |
| Msa0084150 | Msa1133860 | 0.808946 | 2.411633e-50 | -8.615850e-47 |
| Msa0084150 | Msa1164240 | 0.815390 | 9.475954e-52 | -8.615850e-47 |
| Msa0084150 | Msa1170340 | 0.809408 | 1.920210e-50 | -8.615850e-47 |
| Msa0084150 | Msa1179170 | 0.806675 | 7.330605e-50 | -8.615850e-47 |
| Msa0084150 | Msa1182800 | -0.804805 | 1.811302e-49 | -8.615850e-47 |
| Msa0084150 | Msa1216600 | 0.810799 | 9.631350e-51 | -8.615850e-47 |
| Msa0084150 | Msa1233650 | 0.801805 | 7.571057e-49 | -8.615850e-47 |
| Msa0084150 | Msa1255710 | 0.810295 | 1.237774e-50 | -8.615850e-47 |
| Msa0084150 | Msa1269050 | 0.830749 | 2.464284e-55 | -8.615850e-47 |
| Msa0084150 | Msa1296870 | 0.804058 | 2.591731e-49 | -8.615850e-47 |
| Msa0084150 | Msa1300610 | 0.821570 | 3.763270e-53 | -8.615850e-47 |
| Msa0084150 | Msa1319290 | 0.809378 | 1.949413e-50 | -8.615850e-47 |
| Msa0084150 | Msa1323080 | 0.813684 | 2.259890e-51 | -8.615850e-47 |
| Msa0084150 | Msa1323120 | 0.813832 | 2.096023e-51 | -8.615850e-47 |
| Msa0084150 | Msa1329740 | 0.818254 | 2.158071e-52 | -8.615850e-47 |
| Msa0084150 | Msa1339490 | 0.854785 | 9.767259e-62 | -8.615850e-47 |
| Msa0084150 | Msa1343590 | 0.812353 | 4.425119e-51 | -8.615850e-47 |
| Msa0084150 | Msa1357490 | 0.801994 | 6.924150e-49 | -8.615850e-47 |
| Msa0084150 | Msa1357750 | 0.803136 | 4.025247e-49 | -8.615850e-47 |
| Msa0084150 | Msa1376070 | 0.838301 | 3.120206e-57 | -8.615850e-47 |
| Msa0084150 | Msa1377520 | 0.803048 | 4.198051e-49 | -8.615850e-47 |
| Msa0084150 | Msa1378400 | 0.807735 | 4.371294e-50 | -8.615850e-47 |
| Msa0084150 | Msa1378950 | 0.812118 | 4.978557e-51 | -8.615850e-47 |
| Msa0084150 | Msa1380490 | 0.801096 | 1.057753e-48 | -8.615850e-47 |
| Msa0084150 | Msa1386120 | 0.805470 | 1.314124e-49 | -8.615850e-47 |
| Msa0084150 | Msa1407670 | 0.802077 | 6.656610e-49 | -8.615850e-47 |
| Msa0084150 | Msa1409600 | 0.823125 | 1.639159e-53 | -8.615850e-47 |
| Msa0084150 | Msa1411550 | 0.835639 | 1.493027e-56 | -8.615850e-47 |
| Msa0084150 | Msa1420020 | 0.819733 | 9.945653e-53 | -8.615850e-47 |
| Msa0084150 | Msa1421120 | 0.804497 | 2.099601e-49 | -8.615850e-47 |
| Msa0084150 | Msa1427670 | 0.805826 | 1.106737e-49 | -8.615850e-47 |
| Msa0084150 | Msa1449770 | 0.804502 | 2.094352e-49 | -8.615850e-47 |
| Msa0084150 | Msa1452920 | 0.873085 | 1.931713e-67 | -8.615850e-47 |
| Msa0084150 | Msa1459610 | 0.814849 | 1.249504e-51 | -8.615850e-47 |
| Msa0084150 | Msa1461660 | 0.803358 | 3.622144e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0084150 | MtrunA17_Chr1g0197801 | 63.359 | 131 | 32 | 1 | 3 | 133 | 95 | 209 | 4.65e-50 | 160 |
| Msa0084150 | MtrunA17_Chr3g0095851 | 52.448 | 143 | 66 | 2 | 1 | 143 | 112 | 252 | 9.18e-43 | 142 |
| Msa0084150 | MtrunA17_Chr3g0095861 | 52.448 | 143 | 66 | 2 | 1 | 143 | 111 | 251 | 1.32e-42 | 142 |
| Msa0084150 | MtrunA17_Chr1g0197791 | 56.391 | 133 | 56 | 2 | 3 | 135 | 106 | 236 | 1.29e-40 | 136 |
| Msa0084150 | MtrunA17_Chr3g0087611 | 56.391 | 133 | 56 | 2 | 3 | 135 | 122 | 252 | 1.52e-40 | 137 |
| Msa0084150 | MtrunA17_Chr3g0100011 | 53.623 | 138 | 62 | 2 | 4 | 141 | 136 | 271 | 5.55e-40 | 137 |
| Msa0084150 | MtrunA17_Chr3g0096161 | 54.015 | 137 | 60 | 3 | 3 | 138 | 114 | 248 | 1.20e-39 | 134 |
| Msa0084150 | MtrunA17_Chr3g0100021 | 52.518 | 139 | 64 | 2 | 4 | 141 | 97 | 234 | 1.61e-38 | 131 |
| Msa0084150 | MtrunA17_Chr3g0100031 | 52.206 | 136 | 63 | 2 | 3 | 138 | 108 | 241 | 8.51e-37 | 126 |
| Msa0084150 | MtrunA17_Chr1g0197771 | 52.713 | 129 | 60 | 1 | 4 | 132 | 116 | 243 | 1.21e-36 | 126 |
| Msa0084150 | MtrunA17_Chr1g0197761 | 46.970 | 132 | 68 | 2 | 1 | 132 | 113 | 242 | 1.46e-34 | 121 |
| Msa0084150 | MtrunA17_Chr1g0197751 | 48.889 | 135 | 65 | 3 | 6 | 138 | 39 | 171 | 5.33e-32 | 112 |
| Msa0084150 | MtrunA17_Chr8g0367061 | 41.892 | 148 | 65 | 6 | 6 | 139 | 106 | 246 | 1.05e-24 | 95.5 |
| Msa0084150 | MtrunA17_Chr8g0367031 | 42.963 | 135 | 68 | 3 | 6 | 132 | 108 | 241 | 2.43e-24 | 94.4 |
| Msa0084150 | MtrunA17_Chr8g0367051 | 40.000 | 145 | 70 | 5 | 6 | 134 | 106 | 249 | 1.07e-22 | 90.1 |
| Msa0084150 | MtrunA17_Chr8g0367071 | 43.885 | 139 | 66 | 6 | 6 | 134 | 121 | 257 | 2.59e-22 | 91.7 |
| Msa0084150 | MtrunA17_Chr4g0011021 | 39.850 | 133 | 74 | 4 | 4 | 134 | 118 | 246 | 1.57e-20 | 84.7 |
| Msa0084150 | MtrunA17_Chr8g0367041 | 38.519 | 135 | 74 | 4 | 6 | 132 | 108 | 241 | 1.73e-20 | 84.3 |
| Msa0084150 | MtrunA17_Chr5g0411741 | 40.288 | 139 | 69 | 7 | 6 | 132 | 124 | 260 | 3.95e-20 | 83.6 |
| Msa0084150 | MtrunA17_Chr5g0411751 | 41.429 | 140 | 67 | 7 | 6 | 132 | 124 | 261 | 8.37e-20 | 82.8 |
| Msa0084150 | MtrunA17_Chr5g0411761 | 39.286 | 140 | 70 | 7 | 6 | 132 | 131 | 268 | 4.45e-18 | 78.2 |
| Msa0084150 | MtrunA17_Chr5g0411771 | 39.860 | 143 | 65 | 8 | 6 | 132 | 132 | 269 | 5.88e-18 | 77.8 |
| Msa0084150 | MtrunA17_Chr3g0104481 | 37.500 | 136 | 75 | 3 | 6 | 132 | 70 | 204 | 1.07e-16 | 73.9 |
| Msa0084150 | MtrunA17_Chr1g0197811 | 52.239 | 67 | 30 | 1 | 1 | 67 | 11 | 75 | 1.78e-16 | 69.3 |
| Msa0084150 | MtrunA17_Chr7g0274651 | 37.143 | 140 | 72 | 5 | 6 | 132 | 117 | 253 | 1.77e-15 | 72.0 |
| Msa0084150 | MtrunA17_Chr8g0345451 | 40.299 | 134 | 72 | 6 | 4 | 132 | 111 | 241 | 2.04e-15 | 70.9 |
| Msa0084150 | MtrunA17_Chr4g0049611 | 34.043 | 141 | 77 | 7 | 4 | 132 | 110 | 246 | 3.64e-14 | 68.6 |
| Msa0084150 | MtrunA17_Chr1g0197821 | 75.610 | 41 | 10 | 0 | 101 | 141 | 112 | 152 | 5.06e-14 | 65.5 |
| Msa0084150 | MtrunA17_Chr5g0411721 | 34.043 | 141 | 82 | 4 | 6 | 136 | 129 | 268 | 1.11e-13 | 66.2 |
| Msa0084150 | MtrunA17_Chr8g0367021 | 36.735 | 147 | 83 | 5 | 2 | 140 | 104 | 248 | 4.11e-13 | 64.3 |
| Msa0084150 | MtrunA17_Chr8g0366941 | 36.986 | 146 | 79 | 6 | 6 | 141 | 111 | 253 | 4.59e-13 | 64.7 |
| Msa0084150 | MtrunA17_Chr7g0274661 | 35.766 | 137 | 77 | 4 | 6 | 132 | 126 | 261 | 8.22e-12 | 61.6 |
| Msa0084150 | MtrunA17_Chr4g0027601 | 45.000 | 80 | 39 | 2 | 54 | 131 | 138 | 214 | 2.99e-11 | 59.3 |
| Msa0084150 | MtrunA17_Chr4g0049581 | 29.452 | 146 | 90 | 5 | 4 | 138 | 128 | 271 | 3.45e-11 | 59.7 |
| Msa0084150 | MtrunA17_Chr1g0175451 | 33.099 | 142 | 78 | 5 | 5 | 133 | 101 | 238 | 6.83e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0084150 | AT5G65600.1 | 40.714 | 140 | 67 | 6 | 6 | 131 | 117 | 254 | 8.45e-17 | 76.3 |
| Msa0084150 | AT5G10530.1 | 41.727 | 139 | 67 | 6 | 6 | 132 | 100 | 236 | 8.73e-17 | 76.3 |
Find 39 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAACCTTGGAGTTGTTAA+TGG | 0.284776 | 1_2:-63449031 | Msa0084150:CDS |
| AATTCCAACATGTGAGTGTT+TGG | 0.311826 | 1_2:+63448943 | None:intergenic |
| AACAGAGTTGGCAGGAATAT+TGG | 0.331936 | 1_2:+63449057 | None:intergenic |
| TAAAGACAGTGAAATGGAAT+AGG | 0.341311 | 1_2:-63448898 | Msa0084150:CDS |
| GTCCTCACAATCCATTTGTT+GGG | 0.372402 | 1_2:-63449003 | Msa0084150:CDS |
| ATTCCAACATGTGAGTGTTT+GGG | 0.396108 | 1_2:+63448944 | None:intergenic |
| ATACTAGTTCCACTGGTTCA+TGG | 0.408344 | 1_2:-63448970 | Msa0084150:CDS |
| TTTCTTTAAAGACAGTGAAA+TGG | 0.423423 | 1_2:-63448904 | Msa0084150:CDS |
| AAACCAATCCTTACGGTGTT+CGG | 0.435951 | 1_2:+63448746 | None:intergenic |
| ATAGTTGAAAAGGTACCATC+TGG | 0.449708 | 1_2:+63448800 | None:intergenic |
| TCAAACTCTACCCCAACAAA+TGG | 0.455076 | 1_2:+63448992 | None:intergenic |
| CTTCCGAACACCGTAAGGAT+TGG | 0.469222 | 1_2:-63448749 | Msa0084150:CDS |
| AGTCCTCACAATCCATTTGT+TGG | 0.471870 | 1_2:-63449004 | Msa0084150:CDS |
| AAAGACAGTGAAATGGAATA+GGG | 0.472851 | 1_2:-63448897 | Msa0084150:CDS |
| AACTCTGTTGGTGGAAACCT+TGG | 0.476055 | 1_2:-63449043 | Msa0084150:CDS |
| GCAGGAATATTGGCTCGATC+TGG | 0.482568 | 1_2:+63449067 | None:intergenic |
| GAGTTTGATACTAGTTCCAC+TGG | 0.484484 | 1_2:-63448977 | Msa0084150:CDS |
| TACTAGTTCCACTGGTTCAT+GGG | 0.490856 | 1_2:-63448969 | Msa0084150:CDS |
| GACTTGAGCAATAGTTGAAA+AGG | 0.492838 | 1_2:+63448790 | None:intergenic |
| TGTCTTAGAGAGATTGTCAT+AGG | 0.497716 | 1_2:+63448844 | None:intergenic |
| AAAAGAGTTGTTTAGCTGAC+CGG | 0.521308 | 1_2:+63448700 | None:intergenic |
| ATTCCTGCCAACTCTGTTGG+TGG | 0.528629 | 1_2:-63449052 | Msa0084150:CDS |
| AATATTCCTGCCAACTCTGT+TGG | 0.548051 | 1_2:-63449055 | Msa0084150:CDS |
| AAGGTTTCCACCAACAGAGT+TGG | 0.550040 | 1_2:+63449045 | None:intergenic |
| TTTCCACCAACAGAGTTGGC+AGG | 0.550472 | 1_2:+63449049 | None:intergenic |
| ATGAAGACAAGTCCACTAGC+AGG | 0.558627 | 1_2:+63449100 | None:intergenic |
| CAGAGCTTCCGAACACCGTA+AGG | 0.561999 | 1_2:-63448754 | Msa0084150:CDS |
| GCTGCCACATCAAGAGGTGC+CGG | 0.566269 | 1_2:-63448719 | Msa0084150:CDS |
| GGAATATTGGCTCGATCTGG+TGG | 0.573690 | 1_2:+63449070 | None:intergenic |
| GTTGTTGTATATTACCCAGA+TGG | 0.588747 | 1_2:-63448815 | Msa0084150:CDS |
| TCCTCACAATCCATTTGTTG+GGG | 0.590344 | 1_2:-63449002 | Msa0084150:CDS |
| ACCCCAACAAATGGATTGTG+AGG | 0.592855 | 1_2:+63449001 | None:intergenic |
| GATCCCAAACACTCACATGT+TGG | 0.594093 | 1_2:-63448947 | Msa0084150:CDS |
| TGTTTCCATTAACAACTCCA+AGG | 0.599188 | 1_2:+63449026 | None:intergenic |
| GAATAGGGTGAGTGGCGCCT+TGG | 0.611314 | 1_2:-63448882 | Msa0084150:CDS |
| CTGACCGGCACCTCTTGATG+TGG | 0.630323 | 1_2:+63448715 | None:intergenic |
| GTGAAATGGAATAGGGTGAG+TGG | 0.643420 | 1_2:-63448890 | Msa0084150:CDS |
| TAGTTGAAAAGGTACCATCT+GGG | 0.650948 | 1_2:+63448801 | None:intergenic |
| TTTGGGATCCCATGAACCAG+TGG | 0.654947 | 1_2:+63448961 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTCTTTAAAGACAGTGAAA+TGG | - | chr1_2:63448893-63448912 | Msa0084150:CDS | 25.0% |
| TAAAGACAGTGAAATGGAAT+AGG | - | chr1_2:63448899-63448918 | Msa0084150:CDS | 30.0% | |
| AAAGACAGTGAAATGGAATA+GGG | - | chr1_2:63448900-63448919 | Msa0084150:CDS | 30.0% | |
| ! | TCAGCTAAACAACTCTTTTA+TGG | - | chr1_2:63449101-63449120 | Msa0084150:CDS | 30.0% |
| ! | CAGCTAAACAACTCTTTTAT+GGG | - | chr1_2:63449102-63449121 | Msa0084150:CDS | 30.0% |
| TGTTTCCATTAACAACTCCA+AGG | + | chr1_2:63448774-63448793 | None:intergenic | 35.0% | |
| ATTCCAACATGTGAGTGTTT+GGG | + | chr1_2:63448856-63448875 | None:intergenic | 35.0% | |
| AATTCCAACATGTGAGTGTT+TGG | + | chr1_2:63448857-63448876 | None:intergenic | 35.0% | |
| ! | GGAGATTTTTACATTCACCA+AGG | + | chr1_2:63448935-63448954 | None:intergenic | 35.0% |
| TGTCTTAGAGAGATTGTCAT+AGG | + | chr1_2:63448956-63448975 | None:intergenic | 35.0% | |
| GTTGTTGTATATTACCCAGA+TGG | - | chr1_2:63448982-63449001 | Msa0084150:CDS | 35.0% | |
| !! | TAGTTGAAAAGGTACCATCT+GGG | + | chr1_2:63448999-63449018 | None:intergenic | 35.0% |
| !! | ATAGTTGAAAAGGTACCATC+TGG | + | chr1_2:63449000-63449019 | None:intergenic | 35.0% |
| GACTTGAGCAATAGTTGAAA+AGG | + | chr1_2:63449010-63449029 | None:intergenic | 35.0% | |
| AGCAGAAAAACCAATCCTTA+CGG | + | chr1_2:63449061-63449080 | None:intergenic | 35.0% | |
| AAAAGAGTTGTTTAGCTGAC+CGG | + | chr1_2:63449100-63449119 | None:intergenic | 35.0% | |
| AACAGAGTTGGCAGGAATAT+TGG | + | chr1_2:63448743-63448762 | None:intergenic | 40.0% | |
| AATATTCCTGCCAACTCTGT+TGG | - | chr1_2:63448742-63448761 | Msa0084150:CDS | 40.0% | |
| GGAAACCTTGGAGTTGTTAA+TGG | - | chr1_2:63448766-63448785 | Msa0084150:CDS | 40.0% | |
| AGTCCTCACAATCCATTTGT+TGG | - | chr1_2:63448793-63448812 | Msa0084150:CDS | 40.0% | |
| GTCCTCACAATCCATTTGTT+GGG | - | chr1_2:63448794-63448813 | Msa0084150:CDS | 40.0% | |
| ! | TCCTCACAATCCATTTGTTG+GGG | - | chr1_2:63448795-63448814 | Msa0084150:CDS | 40.0% |
| TCAAACTCTACCCCAACAAA+TGG | + | chr1_2:63448808-63448827 | None:intergenic | 40.0% | |
| GAGTTTGATACTAGTTCCAC+TGG | - | chr1_2:63448820-63448839 | Msa0084150:CDS | 40.0% | |
| !! | ATACTAGTTCCACTGGTTCA+TGG | - | chr1_2:63448827-63448846 | Msa0084150:CDS | 40.0% |
| !! | TACTAGTTCCACTGGTTCAT+GGG | - | chr1_2:63448828-63448847 | Msa0084150:CDS | 40.0% |
| AAACCAATCCTTACGGTGTT+CGG | + | chr1_2:63449054-63449073 | None:intergenic | 40.0% | |
| ATGAAGACAAGTCCACTAGC+AGG | + | chr1_2:63448700-63448719 | None:intergenic | 45.0% | |
| AAGGTTTCCACCAACAGAGT+TGG | + | chr1_2:63448755-63448774 | None:intergenic | 45.0% | |
| ! | AACTCTGTTGGTGGAAACCT+TGG | - | chr1_2:63448754-63448773 | Msa0084150:CDS | 45.0% |
| !! | ACCCCAACAAATGGATTGTG+AGG | + | chr1_2:63448799-63448818 | None:intergenic | 45.0% |
| GATCCCAAACACTCACATGT+TGG | - | chr1_2:63448850-63448869 | Msa0084150:CDS | 45.0% | |
| GTGAAATGGAATAGGGTGAG+TGG | - | chr1_2:63448907-63448926 | Msa0084150:CDS | 45.0% | |
| ! | TTTTCTGCTGCCACATCAAG+AGG | - | chr1_2:63449072-63449091 | Msa0084150:CDS | 45.0% |
| !! | GGAATATTGGCTCGATCTGG+TGG | + | chr1_2:63448730-63448749 | None:intergenic | 50.0% |
| !! | GCAGGAATATTGGCTCGATC+TGG | + | chr1_2:63448733-63448752 | None:intergenic | 50.0% |
| ATTCCTGCCAACTCTGTTGG+TGG | - | chr1_2:63448745-63448764 | Msa0084150:CDS | 50.0% | |
| TTTCCACCAACAGAGTTGGC+AGG | + | chr1_2:63448751-63448770 | None:intergenic | 50.0% | |
| ! | TTTGGGATCCCATGAACCAG+TGG | + | chr1_2:63448839-63448858 | None:intergenic | 50.0% |
| CTTCCGAACACCGTAAGGAT+TGG | - | chr1_2:63449048-63449067 | Msa0084150:CDS | 50.0% | |
| CAGAGCTTCCGAACACCGTA+AGG | - | chr1_2:63449043-63449062 | Msa0084150:CDS | 55.0% | |
| GAATAGGGTGAGTGGCGCCT+TGG | - | chr1_2:63448915-63448934 | Msa0084150:CDS | 60.0% | |
| ! | GCTGCCACATCAAGAGGTGC+CGG | - | chr1_2:63449078-63449097 | Msa0084150:CDS | 60.0% |
| CTGACCGGCACCTCTTGATG+TGG | + | chr1_2:63449085-63449104 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_2 | gene | 63448691 | 63449128 | 63448691 | ID=Msa0084150;Name=Msa0084150 |
| chr1_2 | mRNA | 63448691 | 63449128 | 63448691 | ID=Msa0084150-mRNA-1;Parent=Msa0084150;Name=Msa0084150-mRNA-1;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|145 |
| chr1_2 | exon | 63448691 | 63449128 | 63448691 | ID=Msa0084150-mRNA-1:exon:21241;Parent=Msa0084150-mRNA-1 |
| chr1_2 | CDS | 63448691 | 63449128 | 63448691 | ID=Msa0084150-mRNA-1:cds;Parent=Msa0084150-mRNA-1 |
| Gene Sequence |
| Protein sequence |
