AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1333990


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1333990 MtrunA17_Chr4g0073201 94.444 180 7 1 1 180 1 177 2.24e-119 335
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1333990 AT4G30410.2 42.077 183 99 4 1 179 1 180 6.63e-32 113
Msa1333990 AT4G30410.1 42.077 183 99 4 1 179 1 180 6.63e-32 113
Msa1333990 AT5G57780.1 38.764 178 84 7 1 176 2 156 9.73e-28 102

Find 37 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTATTCTCTTTATAGAAAA+AGG 0.151336 8_4:+54275981 Msa1333990:CDS
TAAATCAGCAGAAAGCTTTA+TGG 0.212678 8_4:-54275816 None:intergenic
TCTTCACATAAGAAGGTAAT+AGG 0.223742 8_4:+54275941 Msa1333990:CDS
TTGTTGAAGTATTTGCAATT+AGG 0.256685 8_4:-54275886 None:intergenic
TGAGAGATTCTATATAATCT+AGG 0.292570 8_4:-54276162 None:intergenic
TGTTGAAGTATTTGCAATTA+GGG 0.303984 8_4:-54275885 None:intergenic
TATTGTAAAAGCTAATACTA+TGG 0.318727 8_4:-54276298 None:intergenic
ATTCCTAAGGAAATGGATAA+AGG 0.331543 8_4:+54275736 Msa1333990:CDS
TATATAAGTATATTAATTTA+CGG 0.332984 8_4:-54276244 None:intergenic
TGCTTTCTATACATACATTA+AGG 0.340892 8_4:+54276416 Msa1333990:three_prime_UTR
GTTACCAGGTGGAAAATTCA+TGG 0.380790 8_4:+54276114 Msa1333990:CDS
AGCAAGAATTCCTAAGGAAA+TGG 0.389199 8_4:+54275729 Msa1333990:CDS
ATCTTTGCAAGCTAGAGTTA+TGG 0.428655 8_4:+54276383 Msa1333990:three_prime_UTR
CTTCACATAAGAAGGTAATA+GGG 0.431570 8_4:+54275942 Msa1333990:CDS
TTCCTAAGGAAATGGATAAA+GGG 0.440057 8_4:+54275737 Msa1333990:CDS
CTAAAGACAAAACTACCCTT+TGG 0.442557 8_4:+54275855 Msa1333990:CDS
GACCCTTTATCCATTTCCTT+AGG 0.455134 8_4:-54275739 None:intergenic
GAGAGATTCTATATAATCTA+GGG 0.474006 8_4:-54276161 None:intergenic
TGAAGAGATAAAGTAGAAGA+TGG 0.478192 8_4:-54275479 None:intergenic
AAAGATAAAGTGTAAATTGA+GGG 0.484799 8_4:-54276366 None:intergenic
GTCTTTAGACGTGGAAGCCA+TGG 0.491623 8_4:-54275840 None:intergenic
AACCTCATCTTCACATAAGA+AGG 0.491845 8_4:+54275934 Msa1333990:CDS
CAAAGATAAAGTGTAAATTG+AGG 0.535548 8_4:-54276367 None:intergenic
TGCTCAAGCAAGAATTCCTA+AGG 0.547032 8_4:+54275723 Msa1333990:CDS
TACCTTCTTATGTGAAGATG+AGG 0.560965 8_4:-54275936 None:intergenic
GCTTTCTGCTGATTTAGCCA+TGG 0.566451 8_4:+54275823 Msa1333990:CDS
GATGAGAGATGGAAGCAAAG+TGG 0.576621 8_4:-54275402 None:intergenic
GCTAGAGTTATGGAGGAAAG+AGG 0.582149 8_4:+54276393 Msa1333990:three_prime_UTR
TCTCTTCAAGAGTTTGAGCA+AGG 0.584990 8_4:+54275494 Msa1333990:five_prime_UTR
TTTGCAAGCTAGAGTTATGG+AGG 0.607643 8_4:+54276386 Msa1333990:three_prime_UTR
CTCAAGAGTTTGTTACCAGG+TGG 0.612619 8_4:+54276103 Msa1333990:CDS
AGGCTCAAGAGTTTGTTACC+AGG 0.621754 8_4:+54276100 Msa1333990:CDS
TGGTTAAGAAAAGAACAAGA+AGG 0.644687 8_4:+54276080 Msa1333990:CDS
TCGATTAACGAAGAGTTCTG+AGG 0.651928 8_4:-54276221 None:intergenic
GTTGAGAAGAAATCGTGCAA+AGG 0.662740 8_4:+54276024 Msa1333990:CDS
TACATTAAGGCAATGTACAG+AGG 0.665796 8_4:+54276429 Msa1333990:three_prime_UTR
TCGTGCAAAGGAAAGAGTTG+AGG 0.666359 8_4:+54276036 Msa1333990:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGACGTTTTTTTTTTTTT+TGG - chr8_4:54275451-54275470 None:intergenic 15.0%
!! ATATATGATATAATATGCTA+GGG - chr8_4:54275641-54275660 None:intergenic 15.0%
!!! AATGACGTTTTTTTTTTTTT+TGG - chr8_4:54275451-54275470 None:intergenic 15.0%
!! ATATATGATATAATATGCTA+GGG - chr8_4:54275641-54275660 None:intergenic 15.0%
!! CATATATGATATAATATGCT+AGG - chr8_4:54275642-54275661 None:intergenic 20.0%
!! CAAATTAAAAAAAAATACGC+AGG - chr8_4:54275683-54275702 None:intergenic 20.0%
!! CTTTATAGAAAAAGGATAAT+TGG + chr8_4:54275989-54276008 Msa1333990:CDS 20.0%
!! TATTGTAAAAGCTAATACTA+TGG - chr8_4:54276301-54276320 None:intergenic 20.0%
!! AAAGATAAAGTGTAAATTGA+GGG - chr8_4:54276369-54276388 None:intergenic 20.0%
!! CATATATGATATAATATGCT+AGG - chr8_4:54275642-54275661 None:intergenic 20.0%
!! CAAATTAAAAAAAAATACGC+AGG - chr8_4:54275683-54275702 None:intergenic 20.0%
!! CTTTATAGAAAAAGGATAAT+TGG + chr8_4:54275989-54276008 Msa1333990:CDS 20.0%
!! TATTGTAAAAGCTAATACTA+TGG - chr8_4:54276301-54276320 None:intergenic 20.0%
!! AAAGATAAAGTGTAAATTGA+GGG - chr8_4:54276369-54276388 None:intergenic 20.0%
!! TGTTGAAGTATTTGCAATTA+GGG - chr8_4:54275888-54275907 None:intergenic 25.0%
!! TTGTTGAAGTATTTGCAATT+AGG - chr8_4:54275889-54275908 None:intergenic 25.0%
!! CCTTTTTCTATAAAGAGAAT+AGG - chr8_4:54275984-54276003 None:intergenic 25.0%
! CCTATTCTCTTTATAGAAAA+AGG + chr8_4:54275981-54276000 Msa1333990:CDS 25.0%
! ATAGAAAAAGGATAATTGGA+AGG + chr8_4:54275993-54276012 Msa1333990:CDS 25.0%
! GAGAGATTCTATATAATCTA+GGG - chr8_4:54276164-54276183 None:intergenic 25.0%
! TGAGAGATTCTATATAATCT+AGG - chr8_4:54276165-54276184 None:intergenic 25.0%
! CAAAGATAAAGTGTAAATTG+AGG - chr8_4:54276370-54276389 None:intergenic 25.0%
! TGCTTTCTATACATACATTA+AGG + chr8_4:54276416-54276435 Msa1333990:three_prime_UTR 25.0%
!! TGTTGAAGTATTTGCAATTA+GGG - chr8_4:54275888-54275907 None:intergenic 25.0%
!! TTGTTGAAGTATTTGCAATT+AGG - chr8_4:54275889-54275908 None:intergenic 25.0%
!! CCTTTTTCTATAAAGAGAAT+AGG - chr8_4:54275984-54276003 None:intergenic 25.0%
! CCTATTCTCTTTATAGAAAA+AGG + chr8_4:54275981-54276000 Msa1333990:CDS 25.0%
! ATAGAAAAAGGATAATTGGA+AGG + chr8_4:54275993-54276012 Msa1333990:CDS 25.0%
! GAGAGATTCTATATAATCTA+GGG - chr8_4:54276164-54276183 None:intergenic 25.0%
! TGAGAGATTCTATATAATCT+AGG - chr8_4:54276165-54276184 None:intergenic 25.0%
! CAAAGATAAAGTGTAAATTG+AGG - chr8_4:54276370-54276389 None:intergenic 25.0%
! TGCTTTCTATACATACATTA+AGG + chr8_4:54276416-54276435 Msa1333990:three_prime_UTR 25.0%
TGAAGAGATAAAGTAGAAGA+TGG - chr8_4:54275482-54275501 None:intergenic 30.0%
GGGCTTAATATAGTTATTCT+TGG - chr8_4:54275621-54275640 None:intergenic 30.0%
ATTCCTAAGGAAATGGATAA+AGG + chr8_4:54275736-54275755 Msa1333990:CDS 30.0%
TTCCTAAGGAAATGGATAAA+GGG + chr8_4:54275737-54275756 Msa1333990:CDS 30.0%
TAAATCAGCAGAAAGCTTTA+TGG - chr8_4:54275819-54275838 None:intergenic 30.0%
TCTTCACATAAGAAGGTAAT+AGG + chr8_4:54275941-54275960 Msa1333990:CDS 30.0%
CTTCACATAAGAAGGTAATA+GGG + chr8_4:54275942-54275961 Msa1333990:CDS 30.0%
TGGTTAAGAAAAGAACAAGA+AGG + chr8_4:54276080-54276099 Msa1333990:CDS 30.0%
TGAAGAGATAAAGTAGAAGA+TGG - chr8_4:54275482-54275501 None:intergenic 30.0%
GGGCTTAATATAGTTATTCT+TGG - chr8_4:54275621-54275640 None:intergenic 30.0%
ATTCCTAAGGAAATGGATAA+AGG + chr8_4:54275736-54275755 Msa1333990:CDS 30.0%
TTCCTAAGGAAATGGATAAA+GGG + chr8_4:54275737-54275756 Msa1333990:CDS 30.0%
TAAATCAGCAGAAAGCTTTA+TGG - chr8_4:54275819-54275838 None:intergenic 30.0%
TCTTCACATAAGAAGGTAAT+AGG + chr8_4:54275941-54275960 Msa1333990:CDS 30.0%
CTTCACATAAGAAGGTAATA+GGG + chr8_4:54275942-54275961 Msa1333990:CDS 30.0%
TGGTTAAGAAAAGAACAAGA+AGG + chr8_4:54276080-54276099 Msa1333990:CDS 30.0%
!! GACGAAATTTTGATGAGAGA+TGG - chr8_4:54275416-54275435 None:intergenic 35.0%
AGCAAGAATTCCTAAGGAAA+TGG + chr8_4:54275729-54275748 Msa1333990:CDS 35.0%
CTAAAGACAAAACTACCCTT+TGG + chr8_4:54275855-54275874 Msa1333990:CDS 35.0%
! AATTAGGGCTTTTCTCCAAA+GGG - chr8_4:54275873-54275892 None:intergenic 35.0%
AACCTCATCTTCACATAAGA+AGG + chr8_4:54275934-54275953 Msa1333990:CDS 35.0%
TACCTTCTTATGTGAAGATG+AGG - chr8_4:54275939-54275958 None:intergenic 35.0%
ATCTTTGCAAGCTAGAGTTA+TGG + chr8_4:54276383-54276402 Msa1333990:three_prime_UTR 35.0%
!! GACGAAATTTTGATGAGAGA+TGG - chr8_4:54275416-54275435 None:intergenic 35.0%
AGCAAGAATTCCTAAGGAAA+TGG + chr8_4:54275729-54275748 Msa1333990:CDS 35.0%
CTAAAGACAAAACTACCCTT+TGG + chr8_4:54275855-54275874 Msa1333990:CDS 35.0%
! AATTAGGGCTTTTCTCCAAA+GGG - chr8_4:54275873-54275892 None:intergenic 35.0%
AACCTCATCTTCACATAAGA+AGG + chr8_4:54275934-54275953 Msa1333990:CDS 35.0%
TACCTTCTTATGTGAAGATG+AGG - chr8_4:54275939-54275958 None:intergenic 35.0%
ATCTTTGCAAGCTAGAGTTA+TGG + chr8_4:54276383-54276402 Msa1333990:three_prime_UTR 35.0%
TCTCTTCAAGAGTTTGAGCA+AGG + chr8_4:54275494-54275513 Msa1333990:five_prime_UTR 40.0%
TGCTCAAGCAAGAATTCCTA+AGG + chr8_4:54275723-54275742 Msa1333990:CDS 40.0%
GACCCTTTATCCATTTCCTT+AGG - chr8_4:54275742-54275761 None:intergenic 40.0%
!!! GGTAGTTTTGTCTTTAGACG+TGG - chr8_4:54275852-54275871 None:intergenic 40.0%
! CAATTAGGGCTTTTCTCCAA+AGG - chr8_4:54275874-54275893 None:intergenic 40.0%
GTTGAGAAGAAATCGTGCAA+AGG + chr8_4:54276024-54276043 Msa1333990:CDS 40.0%
GTTACCAGGTGGAAAATTCA+TGG + chr8_4:54276114-54276133 Msa1333990:CDS 40.0%
! TCATCCATGAATTTTCCACC+TGG - chr8_4:54276121-54276140 None:intergenic 40.0%
TCGATTAACGAAGAGTTCTG+AGG - chr8_4:54276224-54276243 None:intergenic 40.0%
TTTGCAAGCTAGAGTTATGG+AGG + chr8_4:54276386-54276405 Msa1333990:three_prime_UTR 40.0%
TCTCTTCAAGAGTTTGAGCA+AGG + chr8_4:54275494-54275513 Msa1333990:five_prime_UTR 40.0%
TGCTCAAGCAAGAATTCCTA+AGG + chr8_4:54275723-54275742 Msa1333990:CDS 40.0%
GACCCTTTATCCATTTCCTT+AGG - chr8_4:54275742-54275761 None:intergenic 40.0%
!!! GGTAGTTTTGTCTTTAGACG+TGG - chr8_4:54275852-54275871 None:intergenic 40.0%
! CAATTAGGGCTTTTCTCCAA+AGG - chr8_4:54275874-54275893 None:intergenic 40.0%
GTTGAGAAGAAATCGTGCAA+AGG + chr8_4:54276024-54276043 Msa1333990:CDS 40.0%
GTTACCAGGTGGAAAATTCA+TGG + chr8_4:54276114-54276133 Msa1333990:CDS 40.0%
! TCATCCATGAATTTTCCACC+TGG - chr8_4:54276121-54276140 None:intergenic 40.0%
TCGATTAACGAAGAGTTCTG+AGG - chr8_4:54276224-54276243 None:intergenic 40.0%
TTTGCAAGCTAGAGTTATGG+AGG + chr8_4:54276386-54276405 Msa1333990:three_prime_UTR 40.0%
!! GCTTTCTGCTGATTTAGCCA+TGG + chr8_4:54275823-54275842 Msa1333990:CDS 45.0%
TCGTGCAAAGGAAAGAGTTG+AGG + chr8_4:54276036-54276055 Msa1333990:CDS 45.0%
!! AGGCGAGTTTTATAGCGACA+CGG + chr8_4:54276056-54276075 Msa1333990:CDS 45.0%
!! GAGTTTTATAGCGACACGGT+TGG + chr8_4:54276060-54276079 Msa1333990:CDS 45.0%
!! AGGCTCAAGAGTTTGTTACC+AGG + chr8_4:54276100-54276119 Msa1333990:CDS 45.0%
!! CTCAAGAGTTTGTTACCAGG+TGG + chr8_4:54276103-54276122 Msa1333990:CDS 45.0%
GCTAGAGTTATGGAGGAAAG+AGG + chr8_4:54276393-54276412 Msa1333990:three_prime_UTR 45.0%
!! GCTTTCTGCTGATTTAGCCA+TGG + chr8_4:54275823-54275842 Msa1333990:CDS 45.0%
TCGTGCAAAGGAAAGAGTTG+AGG + chr8_4:54276036-54276055 Msa1333990:CDS 45.0%
!! AGGCGAGTTTTATAGCGACA+CGG + chr8_4:54276056-54276075 Msa1333990:CDS 45.0%
!! GAGTTTTATAGCGACACGGT+TGG + chr8_4:54276060-54276079 Msa1333990:CDS 45.0%
!! AGGCTCAAGAGTTTGTTACC+AGG + chr8_4:54276100-54276119 Msa1333990:CDS 45.0%
!! CTCAAGAGTTTGTTACCAGG+TGG + chr8_4:54276103-54276122 Msa1333990:CDS 45.0%
GCTAGAGTTATGGAGGAAAG+AGG + chr8_4:54276393-54276412 Msa1333990:three_prime_UTR 45.0%
!! TATATAAGTATATTAATTTA+CGG - chr8_4:54276247-54276266 None:intergenic 5.0%
!! TATATAAGTATATTAATTTA+CGG - chr8_4:54276247-54276266 None:intergenic 5.0%
GTCTTTAGACGTGGAAGCCA+TGG - chr8_4:54275843-54275862 None:intergenic 50.0%
GTCTTTAGACGTGGAAGCCA+TGG - chr8_4:54275843-54275862 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 54275405 54276449 54275405 ID=Msa1333990;Name=Msa1333990
chr8_4 mRNA 54275405 54276449 54275405 ID=Msa1333990-mRNA-1;Parent=Msa1333990;Name=Msa1333990-mRNA-1;_AED=0.00;_eAED=0.00;_QI=111|0|0.5|1|0|0.5|2|200|180
chr8_4 exon 54275405 54275515 54275405 ID=Msa1333990-mRNA-1:exon:16821;Parent=Msa1333990-mRNA-1
chr8_4 exon 54275707 54276449 54275707 ID=Msa1333990-mRNA-1:exon:16822;Parent=Msa1333990-mRNA-1
chr8_4 five_prime_UTR 54275405 54275515 54275405 ID=Msa1333990-mRNA-1:five_prime_utr;Parent=Msa1333990-mRNA-1
chr8_4 CDS 54275707 54276249 54275707 ID=Msa1333990-mRNA-1:cds;Parent=Msa1333990-mRNA-1
chr8_4 three_prime_UTR 54276250 54276449 54276250 ID=Msa1333990-mRNA-1:three_prime_utr;Parent=Msa1333990-mRNA-1
Gene Sequence

>Msa1333990

ATGCGAAATTTGAGCTTGCTCAAGCAAGAATTCCTAAGGAAATGGATAAAGGGTCTAAAAAAATATAACTCTCAAAAGAAGAATATCAACTTGTTCGAGAGAAAGAAAGCCATAAAGCTTTCTGCTGATTTAGCCATGGCTTCCACGTCTAAAGACAAAACTACCCTTTGGAGAAAAGCCCTAATTGCAAATACTTCAACAAGCATAGATGAACAAGACATATCAACAACCTCATCTTCACATAAGAAGGTAATAGGGAAAAACTCGACGAATTCCTATTCTCTTTATAGAAAAAGGATAATTGGAAGGAGAAAGACGTTGAGAAGAAATCGTGCAAAGGAAAGAGTTGAGGCGAGTTTTATAGCGACACGGTTGGTTAAGAAAAGAACAAGAAGGCTCAAGAGTTTGTTACCAGGTGGAAAATTCATGGATGATGCTTGTTTAGTTGATGAAACCCTAGATTATATAGAATCTCTCAAAGCTCAAGTTGAAGTGATGAGATGTCTTGTTACTGCCTCAGAACTCTTCGTTAATCGACCGTAA

Protein sequence

>Msa1333990

MRNLSLLKQEFLRKWIKGLKKYNSQKKNINLFERKKAIKLSADLAMASTSKDKTTLWRKALIANTSTSIDEQDISTTSSSHKKVIGKNSTNSYSLYRKRIIGRRKTLRRNRAKERVEASFIATRLVKKRTRRLKSLLPGGKFMDDACLVDETLDYIESLKAQVEVMRCLVTASELFVNRP*