AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0304700


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0304700 MtrunA17_Chr2g0333051 82.963 135 5 1 1 117 1 135 3.02e-67 199
Msa0304700 MtrunA17_Chr4g0003391 46.565 131 47 4 1 116 1 123 1.17e-23 88.2
Msa0304700 MtrunA17_Chr4g0063791 34.058 138 63 4 5 115 35 171 5.98e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0304700 AT1G52565.1 39.535 129 60 3 1 117 4 126 5.19e-14 63.9
Msa0304700 AT3G15760.1 38.686 137 59 6 1 117 1 132 1.34e-13 63.2

Find 17 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCCATCATTTCAAATTCT+TGG 0.191389 2_3:+62049981 Msa0304700:CDS
TACAAAGCCATTCTATTTGC+AGG 0.298881 2_3:-62050587 None:intergenic
CTTCCAAGAATTTGAAATGA+TGG 0.348228 2_3:-62049984 None:intergenic
GATAAAATACACTGCTTCAT+AGG 0.352001 2_3:-62050683 None:intergenic
TGATGGGTTAATCTTGTGTT+TGG 0.357672 2_3:-62049967 None:intergenic
ATAGAATGGCTTTGTATAAC+GGG 0.410953 2_3:+62050594 Msa0304700:CDS
TTGAATGAAACTTGGGAAAG+AGG 0.436103 2_3:+62050656 Msa0304700:three_prime_UTR
AATAGAATGGCTTTGTATAA+CGG 0.457196 2_3:+62050593 Msa0304700:CDS
TTCCAAGAATTTGAAATGAT+GGG 0.463098 2_3:-62049983 None:intergenic
GATTCTTGTTGAATGAAACT+TGG 0.465140 2_3:+62050648 Msa0304700:exon
ACAAATGACTTCTCTCTCAC+AGG 0.497730 2_3:+62049887 None:intergenic
TAGATTTGAAGAAAGTGTGA+AGG 0.513088 2_3:+62050463 Msa0304700:CDS
TGGAATTCCTGCAAATAGAA+TGG 0.533064 2_3:+62050580 Msa0304700:CDS
ATTCTTGTTGAATGAAACTT+GGG 0.544886 2_3:+62050649 Msa0304700:exon
TTTCTCAGAGGAAAAGAAGA+GGG 0.546313 2_3:+62050403 Msa0304700:intron
CAAATGACTTCTCTCTCACA+GGG 0.592780 2_3:+62049888 None:intergenic
AGTGAAACAACGAAAACTCG+TGG 0.634965 2_3:+62050560 Msa0304700:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCGATATTGATATTAATTA+AGG - chr2_3:62050287-62050306 None:intergenic 15.0%
!! ATCGATATTGATATTAATTA+AGG - chr2_3:62050287-62050306 None:intergenic 15.0%
!! TCTACATACAAAATAAAGTA+AGG - chr2_3:62050091-62050110 None:intergenic 20.0%
!!! TTACTTTATTTTGTATGTAG+AGG + chr2_3:62050090-62050109 Msa0304700:intron 20.0%
!!! ATATCGATATTGTTATTGTT+AGG - chr2_3:62050207-62050226 None:intergenic 20.0%
!! AATATCAATATCGATATTGT+CGG + chr2_3:62050292-62050311 Msa0304700:intron 20.0%
!! AAAATACACACAATATAGTA+AGG - chr2_3:62050383-62050402 None:intergenic 20.0%
!! TCTACATACAAAATAAAGTA+AGG - chr2_3:62050091-62050110 None:intergenic 20.0%
!!! TTACTTTATTTTGTATGTAG+AGG + chr2_3:62050090-62050109 Msa0304700:intron 20.0%
!!! ATATCGATATTGTTATTGTT+AGG - chr2_3:62050207-62050226 None:intergenic 20.0%
!! AATATCAATATCGATATTGT+CGG + chr2_3:62050292-62050311 Msa0304700:intron 20.0%
!! AAAATACACACAATATAGTA+AGG - chr2_3:62050383-62050402 None:intergenic 20.0%
!! TTCCAAGAATTTGAAATGAT+GGG - chr2_3:62049986-62050005 None:intergenic 25.0%
!!! TTTTTTATGACCTAACTCAA+CGG - chr2_3:62050233-62050252 None:intergenic 25.0%
!!! AATAGAATGGCTTTGTATAA+CGG + chr2_3:62050593-62050612 Msa0304700:CDS 25.0%
! ATTCTTGTTGAATGAAACTT+GGG + chr2_3:62050649-62050668 Msa0304700:exon 25.0%
! TCAAAGAAAAACAAAAACAG+AGG - chr2_3:62050726-62050745 None:intergenic 25.0%
!!! TGTTTTTGTTTTTCTTTGAG+TGG + chr2_3:62050727-62050746 Msa0304700:three_prime_UTR 25.0%
!!! TGAATTCTAGTTTAGTTTTG+TGG + chr2_3:62050753-62050772 Msa0304700:three_prime_UTR 25.0%
!! TTCCAAGAATTTGAAATGAT+GGG - chr2_3:62049986-62050005 None:intergenic 25.0%
!!! TTTTTTATGACCTAACTCAA+CGG - chr2_3:62050233-62050252 None:intergenic 25.0%
!!! AATAGAATGGCTTTGTATAA+CGG + chr2_3:62050593-62050612 Msa0304700:CDS 25.0%
! ATTCTTGTTGAATGAAACTT+GGG + chr2_3:62050649-62050668 Msa0304700:exon 25.0%
! TCAAAGAAAAACAAAAACAG+AGG - chr2_3:62050726-62050745 None:intergenic 25.0%
!!! TGTTTTTGTTTTTCTTTGAG+TGG + chr2_3:62050727-62050746 Msa0304700:three_prime_UTR 25.0%
!!! TGAATTCTAGTTTAGTTTTG+TGG + chr2_3:62050753-62050772 Msa0304700:three_prime_UTR 25.0%
AACCCATCATTTCAAATTCT+TGG + chr2_3:62049981-62050000 Msa0304700:CDS 30.0%
! CTTCCAAGAATTTGAAATGA+TGG - chr2_3:62049987-62050006 None:intergenic 30.0%
ATAGTAAGGACTAAGAACTA+AGG - chr2_3:62050369-62050388 None:intergenic 30.0%
!!! TGTGTGTATTTTTTTCTCAG+AGG + chr2_3:62050391-62050410 Msa0304700:intron 30.0%
! TAGATTTGAAGAAAGTGTGA+AGG + chr2_3:62050463-62050482 Msa0304700:CDS 30.0%
TAGCAAAAAAAGAGAAACAG+AGG + chr2_3:62050507-62050526 Msa0304700:CDS 30.0%
!! ATAGAATGGCTTTGTATAAC+GGG + chr2_3:62050594-62050613 Msa0304700:CDS 30.0%
!! TTTGTATAACGGGATTTTGA+GGG + chr2_3:62050604-62050623 Msa0304700:CDS 30.0%
GATTCTTGTTGAATGAAACT+TGG + chr2_3:62050648-62050667 Msa0304700:exon 30.0%
GATAAAATACACTGCTTCAT+AGG - chr2_3:62050686-62050705 None:intergenic 30.0%
!!! TAGTTTAGTTTTGTGGTAGA+GGG + chr2_3:62050760-62050779 Msa0304700:three_prime_UTR 30.0%
AACCCATCATTTCAAATTCT+TGG + chr2_3:62049981-62050000 Msa0304700:CDS 30.0%
! CTTCCAAGAATTTGAAATGA+TGG - chr2_3:62049987-62050006 None:intergenic 30.0%
ATAGTAAGGACTAAGAACTA+AGG - chr2_3:62050369-62050388 None:intergenic 30.0%
!!! TGTGTGTATTTTTTTCTCAG+AGG + chr2_3:62050391-62050410 Msa0304700:intron 30.0%
! TAGATTTGAAGAAAGTGTGA+AGG + chr2_3:62050463-62050482 Msa0304700:CDS 30.0%
TAGCAAAAAAAGAGAAACAG+AGG + chr2_3:62050507-62050526 Msa0304700:CDS 30.0%
!! ATAGAATGGCTTTGTATAAC+GGG + chr2_3:62050594-62050613 Msa0304700:CDS 30.0%
!! TTTGTATAACGGGATTTTGA+GGG + chr2_3:62050604-62050623 Msa0304700:CDS 30.0%
GATTCTTGTTGAATGAAACT+TGG + chr2_3:62050648-62050667 Msa0304700:exon 30.0%
GATAAAATACACTGCTTCAT+AGG - chr2_3:62050686-62050705 None:intergenic 30.0%
!!! TAGTTTAGTTTTGTGGTAGA+GGG + chr2_3:62050760-62050779 Msa0304700:three_prime_UTR 30.0%
GGAAAAAGCTAGATAAAGCA+TGG - chr2_3:62049949-62049968 None:intergenic 35.0%
!! TGATGGGTTAATCTTGTGTT+TGG - chr2_3:62049970-62049989 None:intergenic 35.0%
AGCGCAAAATTTGTGTGTTA+GGG - chr2_3:62050025-62050044 None:intergenic 35.0%
! TTGCGCTCTTGTTTATATTC+AGG + chr2_3:62050038-62050057 Msa0304700:intron 35.0%
AGGTTAATCAAAAGAAGAGC+TGG - chr2_3:62050071-62050090 None:intergenic 35.0%
GTTGTTCTTGACAAAGTGTA+AGG - chr2_3:62050263-62050282 None:intergenic 35.0%
GTTGTTCTTGACAAAGTGTA+AGG - chr2_3:62050263-62050282 None:intergenic 35.0%
! TTTTCTCAGAGGAAAAGAAG+AGG + chr2_3:62050402-62050421 Msa0304700:intron 35.0%
TTTCTCAGAGGAAAAGAAGA+GGG + chr2_3:62050403-62050422 Msa0304700:intron 35.0%
TGGAATTCCTGCAAATAGAA+TGG + chr2_3:62050580-62050599 Msa0304700:CDS 35.0%
TACAAAGCCATTCTATTTGC+AGG - chr2_3:62050590-62050609 None:intergenic 35.0%
!! CTTTGTATAACGGGATTTTG+AGG + chr2_3:62050603-62050622 Msa0304700:CDS 35.0%
TTGAATGAAACTTGGGAAAG+AGG + chr2_3:62050656-62050675 Msa0304700:three_prime_UTR 35.0%
!!! CTAGTTTAGTTTTGTGGTAG+AGG + chr2_3:62050759-62050778 Msa0304700:three_prime_UTR 35.0%
GGAAAAAGCTAGATAAAGCA+TGG - chr2_3:62049949-62049968 None:intergenic 35.0%
!! TGATGGGTTAATCTTGTGTT+TGG - chr2_3:62049970-62049989 None:intergenic 35.0%
AGCGCAAAATTTGTGTGTTA+GGG - chr2_3:62050025-62050044 None:intergenic 35.0%
! TTGCGCTCTTGTTTATATTC+AGG + chr2_3:62050038-62050057 Msa0304700:intron 35.0%
AGGTTAATCAAAAGAAGAGC+TGG - chr2_3:62050071-62050090 None:intergenic 35.0%
GTTGTTCTTGACAAAGTGTA+AGG - chr2_3:62050263-62050282 None:intergenic 35.0%
GTTGTTCTTGACAAAGTGTA+AGG - chr2_3:62050263-62050282 None:intergenic 35.0%
! TTTTCTCAGAGGAAAAGAAG+AGG + chr2_3:62050402-62050421 Msa0304700:intron 35.0%
TTTCTCAGAGGAAAAGAAGA+GGG + chr2_3:62050403-62050422 Msa0304700:intron 35.0%
TGGAATTCCTGCAAATAGAA+TGG + chr2_3:62050580-62050599 Msa0304700:CDS 35.0%
TACAAAGCCATTCTATTTGC+AGG - chr2_3:62050590-62050609 None:intergenic 35.0%
!! CTTTGTATAACGGGATTTTG+AGG + chr2_3:62050603-62050622 Msa0304700:CDS 35.0%
TTGAATGAAACTTGGGAAAG+AGG + chr2_3:62050656-62050675 Msa0304700:three_prime_UTR 35.0%
!!! CTAGTTTAGTTTTGTGGTAG+AGG + chr2_3:62050759-62050778 Msa0304700:three_prime_UTR 35.0%
GAGCGCAAAATTTGTGTGTT+AGG - chr2_3:62050026-62050045 None:intergenic 40.0%
TACTGAAACTCACACGCATA+AGG + chr2_3:62050124-62050143 Msa0304700:intron 40.0%
TCGATATTGTCCGTTGAGTT+AGG + chr2_3:62050220-62050239 Msa0304700:intron 40.0%
! TCGATATTGTCGGTTAAGCT+AGG + chr2_3:62050302-62050321 Msa0304700:intron 40.0%
AGTGAAACAACGAAAACTCG+TGG + chr2_3:62050560-62050579 Msa0304700:CDS 40.0%
GAGCGCAAAATTTGTGTGTT+AGG - chr2_3:62050026-62050045 None:intergenic 40.0%
TACTGAAACTCACACGCATA+AGG + chr2_3:62050124-62050143 Msa0304700:intron 40.0%
TCGATATTGTCCGTTGAGTT+AGG + chr2_3:62050220-62050239 Msa0304700:intron 40.0%
! TCGATATTGTCGGTTAAGCT+AGG + chr2_3:62050302-62050321 Msa0304700:intron 40.0%
AGTGAAACAACGAAAACTCG+TGG + chr2_3:62050560-62050579 Msa0304700:CDS 40.0%
ACACACAACTGTGTGGTACA+GGG + chr2_3:62050156-62050175 Msa0304700:intron 45.0%
!! TTTTGAGGGATCGTGTGCAT+AGG + chr2_3:62050618-62050637 Msa0304700:CDS 45.0%
ACACACAACTGTGTGGTACA+GGG + chr2_3:62050156-62050175 Msa0304700:intron 45.0%
!! TTTTGAGGGATCGTGTGCAT+AGG + chr2_3:62050618-62050637 Msa0304700:CDS 45.0%
CGAACTCACACACAACTGTG+TGG + chr2_3:62050149-62050168 Msa0304700:intron 50.0%
CACACACAACTGTGTGGTAC+AGG + chr2_3:62050155-62050174 Msa0304700:intron 50.0%
TGTGGTACAGGGTTCGAACT+CGG + chr2_3:62050167-62050186 Msa0304700:intron 50.0%
CGAACTCACACACAACTGTG+TGG + chr2_3:62050149-62050168 Msa0304700:intron 50.0%
CACACACAACTGTGTGGTAC+AGG + chr2_3:62050155-62050174 Msa0304700:intron 50.0%
TGTGGTACAGGGTTCGAACT+CGG + chr2_3:62050167-62050186 Msa0304700:intron 50.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 62049891 62050789 62049891 ID=Msa0304700;Name=Msa0304700
chr2_3 mRNA 62049891 62050789 62049891 ID=Msa0304700-mRNA-1;Parent=Msa0304700;Name=Msa0304700-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0|0|0.5|1|0|0.5|2|140|117
chr2_3 exon 62049891 62050005 62049891 ID=Msa0304700-mRNA-1:exon:20634;Parent=Msa0304700-mRNA-1
chr2_3 exon 62050411 62050789 62050411 ID=Msa0304700-mRNA-1:exon:20635;Parent=Msa0304700-mRNA-1
chr2_3 CDS 62049891 62050005 62049891 ID=Msa0304700-mRNA-1:cds;Parent=Msa0304700-mRNA-1
chr2_3 CDS 62050411 62050649 62050411 ID=Msa0304700-mRNA-1:cds;Parent=Msa0304700-mRNA-1
chr2_3 three_prime_UTR 62050650 62050789 62050650 ID=Msa0304700-mRNA-1:three_prime_utr;Parent=Msa0304700-mRNA-1
Gene Sequence

>Msa0304700

ATGACTTCTCTCTCACAGGGTCTTGTTTTCACTACAGCTATGCTTGTATCAAGCACCATGCTTTATCTAGCTTTTTCCAAACACAAGATTAACCCATCATTTCAAATTCTTGGAAAGGAAAAGAAGAGGGAGAGAAAGATGAATACAAAGAAAAATAAAAAGAAAGTTAGATTTGAAGAAAGTGTGAAGGAAGTAAGAGAAAAGAGTGAGATAGCAAAAAAAGAGAAACAGAGGAAACGAAACAGAGTACATAGCAACTGCAGAAGTGAAACAACGAAAACTCGTGGAATTCCTGCAAATAGAATGGCTTTGTATAACGGGATTTTGAGGGATCGTGTGCATAGGATTGAGTGA

Protein sequence

>Msa0304700

MTSLSQGLVFTTAMLVSSTMLYLAFSKHKINPSFQILGKEKKRERKMNTKKNKKKVRFEESVKEVREKSEIAKKEKQRKRNRVHSNCRSETTKTRGIPANRMALYNGILRDRVHRIE*