AlfalfaGEDB | Msa0431410

M. sativa cultivar zhongmu-4 / Msa0431410

KEGG: K08776
GO: GO:0001666 GO:0003674 GO:0003824 GO:0004177 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006508 GO:0006518 GO:0006807 GO:0006810 GO:0006950 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008235 GO:0008237 GO:0008238 GO:0008270 GO:0009056 GO:0009628 GO:0009914 GO:0009926 GO:0009987 GO:0010013 GO:0010817 GO:0016020 GO:0016787 GO:0019538 GO:0031090 GO:0031406 GO:0033218 GO:0033293 GO:0033554 GO:0034641 GO:0036094 GO:0036293 GO:0036294 GO:0042221 GO:0042277 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043177 GO:0043226 GO:0043227 GO:0043603 GO:0044237 GO:0044238 GO:0044248 GO:0044422 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046914 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0060918 GO:0065007 GO:0065008 GO:0070006 GO:0070011 GO:0070482 GO:0070887 GO:0071453 GO:0071456 GO:0071704 GO:0071944 GO:0097159 GO:0140096 GO:1901564 GO:1901565 GO:1901575
PFAM: PF17900.4

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0431410	XP_003603176.1	91.892	74	6	0	1	74	1	74	1.66e-37	143

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0431410	sp\|Q8VZH2\|APM1_ARATH	63.514	74	27	0	1	74	1	74	9.25e-28	107

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0431410	G7J8C0	91.892	74	6	0	1	74	1	74	7.91e-38	143

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003220	Msa0431410	0.828266	9.893864e-55	-8.615850e-47
Msa0003270	Msa0431410	0.823499	1.340000e-53	-8.615850e-47
Msa0010770	Msa0431410	0.812449	4.216105e-51	-8.615850e-47
Msa0012940	Msa0431410	0.803341	3.651350e-49	-8.615850e-47
Msa0039250	Msa0431410	0.818761	1.656121e-52	-8.615850e-47
Msa0041690	Msa0431410	0.825343	4.937670e-54	-8.615850e-47
Msa0047640	Msa0431410	0.814218	1.722943e-51	-8.615850e-47
Msa0054530	Msa0431410	0.829120	6.148454e-55	-8.615850e-47
Msa0064920	Msa0431410	0.821260	4.437557e-53	-8.615850e-47
Msa0069600	Msa0431410	0.807500	4.904526e-50	-8.615850e-47
Msa0074750	Msa0431410	0.820439	6.853902e-53	-8.615850e-47
Msa0091780	Msa0431410	0.814529	1.470794e-51	-8.615850e-47
Msa0092940	Msa0431410	0.851913	6.511166e-61	-8.615850e-47
Msa0116270	Msa0431410	0.803447	3.471045e-49	-8.615850e-47
Msa0126230	Msa0431410	0.809518	1.818597e-50	-8.615850e-47
Msa0131700	Msa0431410	0.823123	1.640868e-53	-8.615850e-47
Msa0133720	Msa0431410	0.828520	8.587791e-55	-8.615850e-47
Msa0133820	Msa0431410	0.826391	2.782995e-54	-8.615850e-47
Msa0138210	Msa0431410	0.829187	5.921494e-55	-8.615850e-47
Msa0146080	Msa0431410	0.801612	8.293675e-49	-8.615850e-47
Msa0183420	Msa0431410	0.819496	1.126523e-52	-8.615850e-47
Msa0387950	Msa0431410	0.824615	7.333798e-54	-8.615850e-47
Msa0395980	Msa0431410	0.807007	6.237671e-50	-8.615850e-47
Msa0423840	Msa0431410	0.817373	3.410552e-52	-8.615850e-47
Msa0431410	Msa0432070	0.846542	2.034723e-59	-8.615850e-47
Msa0431410	Msa0433610	0.816207	6.229845e-52	-8.615850e-47
Msa0431410	Msa0434420	0.803312	3.701197e-49	-8.615850e-47
Msa0431410	Msa0446900	0.804234	2.382703e-49	-8.615850e-47
Msa0431410	Msa0484290	0.806057	9.894548e-50	-8.615850e-47
Msa0431410	Msa0491000	0.805719	1.165340e-49	-8.615850e-47
Msa0431410	Msa0495220	0.803531	3.334567e-49	-8.615850e-47
Msa0431410	Msa0510290	0.823457	1.370896e-53	-8.615850e-47
Msa0431410	Msa0511150	0.829228	5.787197e-55	-8.615850e-47
Msa0431410	Msa0516120	0.800440	1.440105e-48	-8.615850e-47
Msa0431410	Msa0518960	0.831618	1.506857e-55	-8.615850e-47
Msa0431410	Msa0531250	0.813826	2.102923e-51	-8.615850e-47
Msa0431410	Msa0547680	0.808890	2.479108e-50	-8.615850e-47
Msa0431410	Msa0583500	0.815019	1.145257e-51	-8.615850e-47
Msa0431410	Msa0588120	0.819437	1.161825e-52	-8.615850e-47
Msa0431410	Msa0597770	0.817033	4.067123e-52	-8.615850e-47
Msa0431410	Msa0599900	0.805133	1.546589e-49	-8.615850e-47
Msa0431410	Msa0628500	0.814030	1.895966e-51	-8.615850e-47
Msa0431410	Msa0646400	0.801785	7.644589e-49	-8.615850e-47
Msa0431410	Msa0658480	0.802124	6.509615e-49	-8.615850e-47
Msa0431410	Msa0687230	0.813046	3.120379e-51	-8.615850e-47
Msa0431410	Msa0687450	0.813576	2.387314e-51	-8.615850e-47
Msa0431410	Msa0689760	0.808088	3.677563e-50	-8.615850e-47
Msa0431410	Msa0716860	0.824229	9.037151e-54	-8.615850e-47
Msa0431410	Msa0734960	0.803115	4.066010e-49	-8.615850e-47
Msa0431410	Msa0743530	0.815995	6.946416e-52	-8.615850e-47
Msa0431410	Msa0749400	0.834835	2.381337e-56	-8.615850e-47
Msa0431410	Msa0757800	0.802190	6.309351e-49	-8.615850e-47
Msa0431410	Msa0760770	0.805881	1.077826e-49	-8.615850e-47
Msa0431410	Msa0767100	0.809347	1.979366e-50	-8.615850e-47
Msa0431410	Msa0788120	0.806610	7.566236e-50	-8.615850e-47
Msa0431410	Msa0789560	0.816272	6.022160e-52	-8.615850e-47
Msa0431410	Msa0826510	0.821454	4.002983e-53	-8.615850e-47
Msa0431410	Msa0865620	0.824280	8.790316e-54	-8.615850e-47
Msa0431410	Msa0869010	0.809019	2.327436e-50	-8.615850e-47
Msa0431410	Msa0888940	0.801272	9.739194e-49	-8.615850e-47
Msa0431410	Msa0969330	0.826325	2.886027e-54	-8.615850e-47
Msa0431410	Msa0996020	0.806526	7.881603e-50	-8.615850e-47
Msa0431410	Msa1010070	0.801389	9.212936e-49	-8.615850e-47
Msa0431410	Msa1020940	0.804430	2.168294e-49	-8.615850e-47
Msa0431410	Msa1036740	0.805111	1.563158e-49	-8.615850e-47
Msa0431410	Msa1072600	0.833792	4.351505e-56	-8.615850e-47
Msa0431410	Msa1095790	0.852085	5.819446e-61	-8.615850e-47
Msa0431410	Msa1122780	0.808563	2.912377e-50	-8.615850e-47
Msa0431410	Msa1123350	0.819682	1.021410e-52	-8.615850e-47
Msa0431410	Msa1127430	0.807498	4.909070e-50	-8.615850e-47
Msa0431410	Msa1127540	0.865226	6.887205e-65	-8.615850e-47
Msa0431410	Msa1127590	0.808072	3.707301e-50	-8.615850e-47
Msa0431410	Msa1169720	0.800289	1.545493e-48	-8.615850e-47
Msa0431410	Msa1185130	0.801733	7.832956e-49	-8.615850e-47
Msa0431410	Msa1188560	0.815611	8.458250e-52	-8.615850e-47
Msa0431410	Msa1199290	0.807405	5.137423e-50	-8.615850e-47
Msa0431410	Msa1201640	0.824269	8.844586e-54	-8.615850e-47
Msa0431410	Msa1201810	0.815666	8.225582e-52	-8.615850e-47
Msa0431410	Msa1201830	0.813477	2.509535e-51	-8.615850e-47
Msa0431410	Msa1205480	0.830404	2.992856e-55	-8.615850e-47
Msa0431410	Msa1211340	0.828465	8.855114e-55	-8.615850e-47
Msa0431410	Msa1214320	0.815364	9.599810e-52	-8.615850e-47
Msa0431410	Msa1235250	0.800815	1.207552e-48	-8.615850e-47
Msa0431410	Msa1254620	0.834371	3.116462e-56	-8.615850e-47
Msa0431410	Msa1255410	0.801160	1.026431e-48	-8.615850e-47
Msa0431410	Msa1259640	0.820371	7.105440e-53	-8.615850e-47
Msa0431410	Msa1261070	0.821861	3.223390e-53	-8.615850e-47
Msa0431410	Msa1299330	0.818212	2.205126e-52	-8.615850e-47
Msa0431410	Msa1299360	0.807633	4.594349e-50	-8.615850e-47
Msa0431410	Msa1329450	0.812036	5.188366e-51	-8.615850e-47
Msa0431410	Msa1329790	0.822327	2.513983e-53	-8.615850e-47
Msa0431410	Msa1335690	0.802857	4.598426e-49	-8.615850e-47
Msa0431410	Msa1342270	0.815757	7.849504e-52	-8.615850e-47
Msa0431410	Msa1344310	0.814329	1.628707e-51	-8.615850e-47
Msa0431410	Msa1356610	0.817713	2.859012e-52	-8.615850e-47
Msa0431410	Msa1393800	0.823769	1.158648e-53	-8.615850e-47
Msa0431410	Msa1411500	0.829530	4.889601e-55	-8.615850e-47
Msa0431410	Msa1411950	0.824556	7.571802e-54	-8.615850e-47
Msa0431410	Msa1420250	0.808961	2.394904e-50	-8.615850e-47
Msa0431410	Msa1426910	0.835438	1.677666e-56	-8.615850e-47
Msa0431410	Msa1435260	0.814211	1.728818e-51	-8.615850e-47
Msa0431410	Msa1438450	0.826392	2.781351e-54	-8.615850e-47
Msa0431410	Msa1443310	0.811842	5.719388e-51	-8.615850e-47
Msa0431410	Msa1450720	0.812369	4.389765e-51	-8.615850e-47
Msa0431410	Msa1459910	0.826609	2.469934e-54	-8.615850e-47
Msa0431410	Msa1463470	0.805616	1.225072e-49	-8.615850e-47
Msa0431410	Msa1466590	0.806362	8.536536e-50	-8.615850e-47
Msa0431410	Msa1467000	0.818453	1.944816e-52	-8.615850e-47
Msa0244970	Msa0431410	0.806825	6.815591e-50	-8.615850e-47
Msa0260620	Msa0431410	0.802736	4.868868e-49	-8.615850e-47
Msa0279390	Msa0431410	0.830016	3.723651e-55	-8.615850e-47
Msa0280110	Msa0431410	0.802569	5.272593e-49	-8.615850e-47
Msa0281360	Msa0431410	0.832611	8.564979e-56	-8.615850e-47
Msa0307860	Msa0431410	0.808591	2.872837e-50	-8.615850e-47
Msa0318880	Msa0431410	0.834792	2.441975e-56	-8.615850e-47
Msa0322590	Msa0431410	0.809007	2.341074e-50	-8.615850e-47
Msa0322900	Msa0431410	0.811394	7.155072e-51	-8.615850e-47
Msa0363060	Msa0431410	0.833743	4.475082e-56	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0431410	MtrunA17_Chr3g0135971	91.892	74	6	0	1	74	1	74	1.52e-41	143
Msa0431410	MtrunA17_Chr8g0343081	50.746	67	31	1	1	67	7	71	8.83e-17	72.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0431410	AT4G33090.1	63.514	74	27	0	1	74	1	74	9.41e-29	107

Find 17 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACGGTTTGGAACAGCGAATT+TGG	0.186692	3_2:+83475735	None:intergenic
CACTAATCATGATTCCTCTA+AGG	0.309827	3_2:-83475572	Msa0431410:intron
AGCATTGAGAACGATGAAAT+TGG	0.382062	3_2:+83475630	None:intergenic
AAACGACATTCGACGAGGTC+TGG	0.393141	3_2:+83475694	None:intergenic
ATTGAAAGTAAGTACCTTAG+AGG	0.402691	3_2:+83475558	None:intergenic
CTCGTCGAATGTCGTTTCTC+TGG	0.408745	3_2:-83475688	Msa0431410:CDS
TGGTAGTGGTGACGATGTTA+AGG	0.419984	3_2:+83475650	None:intergenic
AGTCGAATGTCGTAACGGTT+TGG	0.424820	3_2:+83475721	None:intergenic
CGGTTTGGAACAGCGAATTT+GGG	0.440056	3_2:+83475736	None:intergenic
GGTGACGATGTTAAGGTTAA+CGG	0.443284	3_2:+83475657	None:intergenic
AAGAAAATGGATCAATTCAA+AGG	0.534213	3_2:-83475772	None:intergenic
ATCATGATTAGTGAAAGAAA+CGG	0.621284	3_2:+83475582	None:intergenic
GTTTGAGTCGAATGTCGTAA+CGG	0.623074	3_2:+83475716	None:intergenic
GAGAACGATGAAATTGGTAG+TGG	0.630665	3_2:+83475636	None:intergenic
AGAAACGGCGTCGTTAGCGA+CGG	0.649627	3_2:+83475597	None:intergenic
ACAGCGAATTTGGGAAGACG+AGG	0.687895	3_2:+83475745	None:intergenic
CAGAGAAACGACATTCGACG+AGG	0.707634	3_2:+83475689	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTGTGTTTTTTGTTTGTTT+CGG	-	chr3_2:83475728-83475747	Msa0431410:CDS	20.0%
!	ATCATGATTAGTGAAAGAAA+CGG	+	chr3_2:83475656-83475675	None:intergenic	25.0%
	ATTGAAAGTAAGTACCTTAG+AGG	+	chr3_2:83475680-83475699	None:intergenic	30.0%
!	AGCATTGAGAACGATGAAAT+TGG	+	chr3_2:83475608-83475627	None:intergenic	35.0%
	CACTAATCATGATTCCTCTA+AGG	-	chr3_2:83475663-83475682	Msa0431410:CDS	35.0%
	ATCGAGTGAATGTTTCTTGT+AGG	-	chr3_2:83475758-83475777	Msa0431410:CDS	35.0%
	GTTTGAGTCGAATGTCGTAA+CGG	+	chr3_2:83475522-83475541	None:intergenic	40.0%
	GGTGACGATGTTAAGGTTAA+CGG	+	chr3_2:83475581-83475600	None:intergenic	40.0%
	GAGAACGATGAAATTGGTAG+TGG	+	chr3_2:83475602-83475621	None:intergenic	40.0%
	ACTTTCAATCGCTACTTGTG+TGG	-	chr3_2:83475691-83475710	Msa0431410:CDS	40.0%
!!!	TCGCTACTTGTGTGGTTTTT+TGG	-	chr3_2:83475699-83475718	Msa0431410:CDS	40.0%
!	CGGTTTGGAACAGCGAATTT+GGG	+	chr3_2:83475502-83475521	None:intergenic	45.0%
!	ACGGTTTGGAACAGCGAATT+TGG	+	chr3_2:83475503-83475522	None:intergenic	45.0%
	AGTCGAATGTCGTAACGGTT+TGG	+	chr3_2:83475517-83475536	None:intergenic	45.0%
!!	TGGTAGTGGTGACGATGTTA+AGG	+	chr3_2:83475588-83475607	None:intergenic	45.0%
	ACAGCGAATTTGGGAAGACG+AGG	+	chr3_2:83475493-83475512	None:intergenic	50.0%
	AAACGACATTCGACGAGGTC+TGG	+	chr3_2:83475544-83475563	None:intergenic	50.0%
	CAGAGAAACGACATTCGACG+AGG	+	chr3_2:83475549-83475568	None:intergenic	50.0%
!	CTCGTCGAATGTCGTTTCTC+TGG	-	chr3_2:83475547-83475566	Msa0431410:intron	50.0%
	AGAAACGGCGTCGTTAGCGA+CGG	+	chr3_2:83475641-83475660	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	83475469	83475788	83475469	ID=Msa0431410;Name=Msa0431410
chr3_2	mRNA	83475469	83475788	83475469	ID=Msa0431410-mRNA-1;Parent=Msa0431410;Name=Msa0431410-mRNA-1;_AED=0.47;_eAED=0.49;_QI=0\|0\|0\|1\|1\|1\|2\|0\|74
chr3_2	exon	83475573	83475788	83475573	ID=Msa0431410-mRNA-1:exon:23357;Parent=Msa0431410-mRNA-1
chr3_2	exon	83475469	83475477	83475469	ID=Msa0431410-mRNA-1:exon:23356;Parent=Msa0431410-mRNA-1
chr3_2	CDS	83475573	83475788	83475573	ID=Msa0431410-mRNA-1:cds;Parent=Msa0431410-mRNA-1
chr3_2	CDS	83475469	83475477	83475469	ID=Msa0431410-mRNA-1:cds;Parent=Msa0431410-mRNA-1

Gene Sequence	>Msa0431410 ATGGATCAATTCAAAGGACAACCTCGTCTTCCCAAATTCGCTGTTCCAAACCGTTACGACATTCGACTCAAACCAGACCTCGTCGAATGTCGTTTCTCTGGTTCTGTATCCGTTAACCTTAACATCGTCACCACTACCAATTTCATCGTTCTCAATGCTGCTGAACTCACCGTCGCTAACGACGCCGTTTCTTTCACTAATCATGATTCCTCTAAGGTTTTTTAA
Protein sequence	>Msa0431410 MDQFKGQPRLPKFAVPNRYDIRLKPDLVECRFSGSVSVNLNIVTTTNFIVLNAAELTVANDAVSFTNHDSSKVF*