Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87054.t1 | XP_024626508.1 | 97.6 | 127 | 3 | 0 | 23 | 149 | 1 | 127 | 1.80E-58 | 235.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87054.t1 | G7KX21 | 97.6 | 127 | 3 | 0 | 23 | 149 | 6 | 132 | 1.3e-58 | 235.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene87054 | 0.844323 | 8.12E-59 | -1.69E-46 |
| MS.gene049217 | MS.gene87054 | 0.810876 | 9.27E-51 | -1.69E-46 |
| MS.gene049222 | MS.gene87054 | 0.874539 | 6.24E-68 | -1.69E-46 |
| MS.gene049232 | MS.gene87054 | 0.883946 | 2.92E-71 | -1.69E-46 |
| MS.gene049285 | MS.gene87054 | 0.855735 | 5.17E-62 | -1.69E-46 |
| MS.gene049320 | MS.gene87054 | 0.859668 | 3.53E-63 | -1.69E-46 |
| MS.gene049396 | MS.gene87054 | 0.812577 | 3.95E-51 | -1.69E-46 |
| MS.gene049609 | MS.gene87054 | 0.83444 | 2.99E-56 | -1.69E-46 |
| MS.gene049633 | MS.gene87054 | 0.870084 | 1.91E-66 | -1.69E-46 |
| MS.gene049656 | MS.gene87054 | 0.814719 | 1.33E-51 | -1.69E-46 |
| MS.gene049657 | MS.gene87054 | 0.80931 | 2.02E-50 | -1.69E-46 |
| MS.gene049865 | MS.gene87054 | 0.823732 | 1.18E-53 | -1.69E-46 |
| MS.gene05010 | MS.gene87054 | 0.87527 | 3.52E-68 | -1.69E-46 |
| MS.gene050163 | MS.gene87054 | 0.826797 | 2.23E-54 | -1.69E-46 |
| MS.gene050165 | MS.gene87054 | 0.834286 | 3.27E-56 | -1.69E-46 |
| MS.gene050170 | MS.gene87054 | 0.884954 | 1.23E-71 | -1.69E-46 |
| MS.gene050216 | MS.gene87054 | 0.848393 | 6.31E-60 | -1.69E-46 |
| MS.gene050323 | MS.gene87054 | 0.880981 | 3.51E-70 | -1.69E-46 |
| MS.gene050333 | MS.gene87054 | 0.816687 | 4.86E-52 | -1.69E-46 |
| MS.gene050381 | MS.gene87054 | -0.819614 | 1.06E-52 | -1.69E-46 |
| MS.gene050496 | MS.gene87054 | 0.865156 | 7.24E-65 | -1.69E-46 |
| MS.gene050499 | MS.gene87054 | 0.819143 | 1.36E-52 | -1.69E-46 |
| MS.gene050502 | MS.gene87054 | 0.82299 | 1.76E-53 | -1.69E-46 |
| MS.gene050504 | MS.gene87054 | 0.88225 | 1.22E-70 | -1.69E-46 |
| MS.gene050518 | MS.gene87054 | 0.867412 | 1.40E-65 | -1.69E-46 |
| MS.gene050538 | MS.gene87054 | 0.816733 | 4.75E-52 | -1.69E-46 |
| MS.gene050577 | MS.gene87054 | 0.8456 | 3.67E-59 | -1.69E-46 |
| MS.gene050612 | MS.gene87054 | 0.874436 | 6.77E-68 | -1.69E-46 |
| MS.gene050647 | MS.gene87054 | -0.830376 | 3.04E-55 | -1.69E-46 |
| MS.gene050675 | MS.gene87054 | 0.847391 | 1.19E-59 | -1.69E-46 |
| MS.gene050697 | MS.gene87054 | 0.814867 | 1.24E-51 | -1.69E-46 |
| MS.gene050699 | MS.gene87054 | 0.818511 | 1.89E-52 | -1.69E-46 |
| MS.gene050747 | MS.gene87054 | 0.841715 | 4.02E-58 | -1.69E-46 |
| MS.gene050772 | MS.gene87054 | 0.850012 | 2.23E-60 | -1.69E-46 |
| MS.gene05080 | MS.gene87054 | 0.880606 | 4.78E-70 | -1.69E-46 |
| MS.gene050868 | MS.gene87054 | 0.898896 | 3.38E-77 | -1.69E-46 |
| MS.gene050871 | MS.gene87054 | 0.900321 | 8.24E-78 | -1.69E-46 |
| MS.gene050876 | MS.gene87054 | 0.836569 | 8.67E-57 | -1.69E-46 |
| MS.gene050904 | MS.gene87054 | 0.869628 | 2.69E-66 | -1.69E-46 |
| MS.gene050950 | MS.gene87054 | 0.846137 | 2.62E-59 | -1.69E-46 |
| MS.gene050986 | MS.gene87054 | 0.868562 | 5.95E-66 | -1.69E-46 |
| MS.gene051127 | MS.gene87054 | -0.811974 | 5.35E-51 | -1.69E-46 |
| MS.gene051138 | MS.gene87054 | -0.805328 | 1.41E-49 | -1.69E-46 |
| MS.gene051268 | MS.gene87054 | 0.876731 | 1.11E-68 | -1.69E-46 |
| MS.gene051317 | MS.gene87054 | 0.843398 | 1.44E-58 | -1.69E-46 |
| MS.gene051509 | MS.gene87054 | 0.840331 | 9.27E-58 | -1.69E-46 |
| MS.gene051551 | MS.gene87054 | 0.818185 | 2.24E-52 | -1.69E-46 |
| MS.gene051734 | MS.gene87054 | 0.804279 | 2.33E-49 | -1.69E-46 |
| MS.gene051810 | MS.gene87054 | 0.818062 | 2.38E-52 | -1.69E-46 |
| MS.gene051838 | MS.gene87054 | 0.828117 | 1.07E-54 | -1.69E-46 |
| MS.gene051874 | MS.gene87054 | 0.8218 | 3.33E-53 | -1.69E-46 |
| MS.gene051941 | MS.gene87054 | 0.807178 | 5.74E-50 | -1.69E-46 |
| MS.gene051946 | MS.gene87054 | -0.815092 | 1.10E-51 | -1.69E-46 |
| MS.gene052066 | MS.gene87054 | 0.84251 | 2.47E-58 | -1.69E-46 |
| MS.gene052164 | MS.gene87054 | 0.883262 | 5.21E-71 | -1.69E-46 |
| MS.gene052482 | MS.gene87054 | 0.868438 | 6.53E-66 | -1.69E-46 |
| MS.gene052486 | MS.gene87054 | 0.877078 | 8.39E-69 | -1.69E-46 |
| MS.gene052494 | MS.gene87054 | -0.801738 | 7.81E-49 | -1.69E-46 |
| MS.gene052503 | MS.gene87054 | 0.826288 | 2.95E-54 | -1.69E-46 |
| MS.gene052506 | MS.gene87054 | 0.808905 | 2.46E-50 | -1.69E-46 |
| MS.gene052615 | MS.gene87054 | 0.803067 | 4.16E-49 | -1.69E-46 |
| MS.gene052822 | MS.gene87054 | 0.814307 | 1.65E-51 | -1.69E-46 |
| MS.gene052823 | MS.gene87054 | 0.824369 | 8.38E-54 | -1.69E-46 |
| MS.gene052825 | MS.gene87054 | 0.821445 | 4.02E-53 | -1.69E-46 |
| MS.gene052966 | MS.gene87054 | 0.89736 | 1.52E-76 | -1.69E-46 |
| MS.gene052986 | MS.gene87054 | 0.80801 | 3.82E-50 | -1.69E-46 |
| MS.gene052987 | MS.gene87054 | 0.812516 | 4.08E-51 | -1.69E-46 |
| MS.gene053203 | MS.gene87054 | 0.849326 | 3.48E-60 | -1.69E-46 |
| MS.gene053207 | MS.gene87054 | 0.812909 | 3.34E-51 | -1.69E-46 |
| MS.gene053213 | MS.gene87054 | 0.840591 | 7.93E-58 | -1.69E-46 |
| MS.gene053286 | MS.gene87054 | 0.848732 | 5.08E-60 | -1.69E-46 |
| MS.gene053420 | MS.gene87054 | -0.838513 | 2.75E-57 | -1.69E-46 |
| MS.gene053436 | MS.gene87054 | 0.817157 | 3.81E-52 | -1.69E-46 |
| MS.gene053479 | MS.gene87054 | 0.819154 | 1.35E-52 | -1.69E-46 |
| MS.gene053480 | MS.gene87054 | 0.810479 | 1.13E-50 | -1.69E-46 |
| MS.gene053574 | MS.gene87054 | 0.865192 | 7.06E-65 | -1.69E-46 |
| MS.gene053626 | MS.gene87054 | 0.844965 | 5.45E-59 | -1.69E-46 |
| MS.gene053633 | MS.gene87054 | 0.833281 | 5.84E-56 | -1.69E-46 |
| MS.gene053824 | MS.gene87054 | 0.855175 | 7.52E-62 | -1.69E-46 |
| MS.gene053916 | MS.gene87054 | 0.834162 | 3.51E-56 | -1.69E-46 |
| MS.gene054003 | MS.gene87054 | 0.821963 | 3.05E-53 | -1.69E-46 |
| MS.gene054408 | MS.gene87054 | 0.843597 | 1.27E-58 | -1.69E-46 |
| MS.gene054595 | MS.gene87054 | 0.850421 | 1.72E-60 | -1.69E-46 |
| MS.gene054600 | MS.gene87054 | 0.845719 | 3.41E-59 | -1.69E-46 |
| MS.gene054642 | MS.gene87054 | 0.846673 | 1.87E-59 | -1.69E-46 |
| MS.gene054706 | MS.gene87054 | -0.833162 | 6.25E-56 | -1.69E-46 |
| MS.gene054707 | MS.gene87054 | -0.807022 | 6.19E-50 | -1.69E-46 |
| MS.gene054708 | MS.gene87054 | -0.819597 | 1.07E-52 | -1.69E-46 |
| MS.gene05470 | MS.gene87054 | 0.86196 | 7.10E-64 | -1.69E-46 |
| MS.gene054927 | MS.gene87054 | 0.805913 | 1.06E-49 | -1.69E-46 |
| MS.gene05494 | MS.gene87054 | 0.823011 | 1.74E-53 | -1.69E-46 |
| MS.gene054954 | MS.gene87054 | 0.867652 | 1.17E-65 | -1.69E-46 |
| MS.gene055154 | MS.gene87054 | -0.80523 | 1.48E-49 | -1.69E-46 |
| MS.gene055202 | MS.gene87054 | -0.832314 | 1.01E-55 | -1.69E-46 |
| MS.gene055224 | MS.gene87054 | 0.838607 | 2.60E-57 | -1.69E-46 |
| MS.gene055362 | MS.gene87054 | 0.859352 | 4.39E-63 | -1.69E-46 |
| MS.gene055502 | MS.gene87054 | 0.853621 | 2.12E-61 | -1.69E-46 |
| MS.gene055577 | MS.gene87054 | 0.872841 | 2.33E-67 | -1.69E-46 |
| MS.gene055722 | MS.gene87054 | 0.812166 | 4.86E-51 | -1.69E-46 |
| MS.gene055748 | MS.gene87054 | 0.865918 | 4.17E-65 | -1.69E-46 |
| MS.gene055750 | MS.gene87054 | 0.863167 | 3.02E-64 | -1.69E-46 |
| MS.gene055751 | MS.gene87054 | 0.872592 | 2.83E-67 | -1.69E-46 |
| MS.gene055776 | MS.gene87054 | 0.859106 | 5.20E-63 | -1.69E-46 |
| MS.gene05589 | MS.gene87054 | 0.90984 | 3.65E-82 | -1.69E-46 |
| MS.gene055941 | MS.gene87054 | 0.81324 | 2.83E-51 | -1.69E-46 |
| MS.gene056040 | MS.gene87054 | 0.818909 | 1.53E-52 | -1.69E-46 |
| MS.gene056046 | MS.gene87054 | 0.84406 | 9.55E-59 | -1.69E-46 |
| MS.gene056166 | MS.gene87054 | 0.859679 | 3.50E-63 | -1.69E-46 |
| MS.gene056283 | MS.gene87054 | 0.813074 | 3.08E-51 | -1.69E-46 |
| MS.gene056387 | MS.gene87054 | 0.84912 | 3.97E-60 | -1.69E-46 |
| MS.gene056422 | MS.gene87054 | 0.804112 | 2.53E-49 | -1.69E-46 |
| MS.gene056446 | MS.gene87054 | 0.827086 | 1.90E-54 | -1.69E-46 |
| MS.gene056450 | MS.gene87054 | 0.821078 | 4.89E-53 | -1.69E-46 |
| MS.gene056504 | MS.gene87054 | 0.910867 | 1.16E-82 | -1.69E-46 |
| MS.gene056510 | MS.gene87054 | 0.880771 | 4.17E-70 | -1.69E-46 |
| MS.gene056657 | MS.gene87054 | 0.852927 | 3.35E-61 | -1.69E-46 |
| MS.gene056692 | MS.gene87054 | 0.826179 | 3.13E-54 | -1.69E-46 |
| MS.gene056878 | MS.gene87054 | 0.826892 | 2.11E-54 | -1.69E-46 |
| MS.gene056946 | MS.gene87054 | 0.801581 | 8.42E-49 | -1.69E-46 |
| MS.gene057076 | MS.gene87054 | 0.830334 | 3.11E-55 | -1.69E-46 |
| MS.gene057086 | MS.gene87054 | 0.824463 | 7.96E-54 | -1.69E-46 |
| MS.gene057658 | MS.gene87054 | 0.801918 | 7.18E-49 | -1.69E-46 |
| MS.gene057687 | MS.gene87054 | 0.802272 | 6.07E-49 | -1.69E-46 |
| MS.gene057734 | MS.gene87054 | 0.808222 | 3.44E-50 | -1.69E-46 |
| MS.gene057831 | MS.gene87054 | 0.825208 | 5.31E-54 | -1.69E-46 |
| MS.gene057872 | MS.gene87054 | 0.828562 | 8.39E-55 | -1.69E-46 |
| MS.gene05787 | MS.gene87054 | 0.853305 | 2.61E-61 | -1.69E-46 |
| MS.gene058130 | MS.gene87054 | 0.842326 | 2.77E-58 | -1.69E-46 |
| MS.gene058157 | MS.gene87054 | 0.848068 | 7.76E-60 | -1.69E-46 |
| MS.gene058171 | MS.gene87054 | -0.844628 | 6.72E-59 | -1.69E-46 |
| MS.gene058173 | MS.gene87054 | -0.837117 | 6.28E-57 | -1.69E-46 |
| MS.gene058175 | MS.gene87054 | -0.84571 | 3.43E-59 | -1.69E-46 |
| MS.gene058334 | MS.gene87054 | -0.825741 | 3.97E-54 | -1.69E-46 |
| MS.gene058338 | MS.gene87054 | 0.813489 | 2.49E-51 | -1.69E-46 |
| MS.gene05837 | MS.gene87054 | 0.877712 | 5.04E-69 | -1.69E-46 |
| MS.gene058419 | MS.gene87054 | 0.850613 | 1.52E-60 | -1.69E-46 |
| MS.gene058544 | MS.gene87054 | 0.825316 | 5.01E-54 | -1.69E-46 |
| MS.gene058773 | MS.gene87054 | 0.869182 | 3.75E-66 | -1.69E-46 |
| MS.gene058838 | MS.gene87054 | 0.830106 | 3.54E-55 | -1.69E-46 |
| MS.gene058978 | MS.gene87054 | -0.810662 | 1.03E-50 | -1.69E-46 |
| MS.gene059022 | MS.gene87054 | 0.871186 | 8.28E-67 | -1.69E-46 |
| MS.gene059023 | MS.gene87054 | 0.85858 | 7.47E-63 | -1.69E-46 |
| MS.gene05913 | MS.gene87054 | 0.836821 | 7.47E-57 | -1.69E-46 |
| MS.gene059305 | MS.gene87054 | 0.813892 | 2.03E-51 | -1.69E-46 |
| MS.gene059367 | MS.gene87054 | 0.86275 | 4.06E-64 | -1.69E-46 |
| MS.gene059368 | MS.gene87054 | 0.803947 | 2.73E-49 | -1.69E-46 |
| MS.gene059526 | MS.gene87054 | -0.819337 | 1.22E-52 | -1.69E-46 |
| MS.gene059667 | MS.gene87054 | 0.85551 | 6.01E-62 | -1.69E-46 |
| MS.gene059677 | MS.gene87054 | 0.84836 | 6.44E-60 | -1.69E-46 |
| MS.gene059680 | MS.gene87054 | 0.804761 | 1.85E-49 | -1.69E-46 |
| MS.gene059718 | MS.gene87054 | 0.856759 | 2.59E-62 | -1.69E-46 |
| MS.gene059801 | MS.gene87054 | 0.830086 | 3.58E-55 | -1.69E-46 |
| MS.gene059837 | MS.gene87054 | 0.829181 | 5.94E-55 | -1.69E-46 |
| MS.gene059865 | MS.gene87054 | 0.900667 | 5.82E-78 | -1.69E-46 |
| MS.gene06000 | MS.gene87054 | 0.86574 | 4.74E-65 | -1.69E-46 |
| MS.gene060187 | MS.gene87054 | -0.808335 | 3.26E-50 | -1.69E-46 |
| MS.gene060295 | MS.gene87054 | 0.801395 | 9.19E-49 | -1.69E-46 |
| MS.gene060372 | MS.gene87054 | 0.808575 | 2.90E-50 | -1.69E-46 |
| MS.gene060482 | MS.gene87054 | 0.829386 | 5.30E-55 | -1.69E-46 |
| MS.gene060593 | MS.gene87054 | -0.810503 | 1.12E-50 | -1.69E-46 |
| MS.gene060595 | MS.gene87054 | -0.859145 | 5.07E-63 | -1.69E-46 |
| MS.gene060703 | MS.gene87054 | 0.848505 | 5.87E-60 | -1.69E-46 |
| MS.gene060762 | MS.gene87054 | 0.889552 | 2.19E-73 | -1.69E-46 |
| MS.gene060778 | MS.gene87054 | 0.899035 | 2.95E-77 | -1.69E-46 |
| MS.gene06077 | MS.gene87054 | 0.888541 | 5.39E-73 | -1.69E-46 |
| MS.gene060835 | MS.gene87054 | 0.815769 | 7.80E-52 | -1.69E-46 |
| MS.gene06092 | MS.gene87054 | 0.818246 | 2.17E-52 | -1.69E-46 |
| MS.gene06097 | MS.gene87054 | 0.827575 | 1.45E-54 | -1.69E-46 |
| MS.gene060982 | MS.gene87054 | 0.902128 | 1.33E-78 | -1.69E-46 |
| MS.gene061044 | MS.gene87054 | -0.822194 | 2.70E-53 | -1.69E-46 |
| MS.gene061150 | MS.gene87054 | 0.804313 | 2.29E-49 | -1.69E-46 |
| MS.gene061218 | MS.gene87054 | 0.802086 | 6.63E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87054.t1 | MTR_7g072470 | 97.638 | 127 | 3 | 0 | 23 | 149 | 6 | 132 | 1.68e-87 | 251 |
| MS.gene87054.t1 | MTR_5g083810 | 56.209 | 153 | 62 | 2 | 1 | 148 | 25 | 177 | 2.59e-50 | 159 |
| MS.gene87054.t1 | MTR_3g466150 | 47.682 | 151 | 72 | 2 | 1 | 144 | 1 | 151 | 4.73e-37 | 124 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87054.t1 | AT2G40435 | 53.378 | 148 | 64 | 2 | 1 | 143 | 1 | 148 | 5.30e-47 | 150 |
| MS.gene87054.t1 | AT3G56220 | 54.225 | 142 | 64 | 1 | 8 | 148 | 13 | 154 | 4.42e-46 | 147 |
| MS.gene87054.t1 | AT2G40435 | 56.800 | 125 | 49 | 2 | 1 | 120 | 1 | 125 | 3.83e-42 | 137 |
| MS.gene87054.t1 | AT2G40435 | 56.800 | 125 | 49 | 2 | 1 | 120 | 1 | 125 | 3.97e-42 | 136 |
| MS.gene87054.t1 | AT3G56220 | 57.522 | 113 | 48 | 0 | 8 | 120 | 13 | 125 | 1.00e-39 | 130 |
Find 25 sgRNAs with CRISPR-Local
Find 107 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCAACTCTTCTATGTATT+TGG | 0.128186 | 7.4:+32127018 | None:intergenic |
| TTTCATGATTAATGTGCTTT+TGG | 0.236031 | 7.4:-32126717 | MS.gene87054:CDS |
| TGGTATGCTGGTATCAATAC+TGG | 0.411863 | 7.4:-32126681 | MS.gene87054:CDS |
| CAACTCTTCTATGTATTTGG+AGG | 0.422808 | 7.4:+32127021 | None:intergenic |
| GAGTGTGAAAACCCTAGAAA+AGG | 0.453059 | 7.4:-32126741 | MS.gene87054:CDS |
| GAGGGAATGAACTCTGAGTT+AGG | 0.454266 | 7.4:-32126986 | MS.gene87054:CDS |
| CTCCTCCCACAGCTTCTAGC+TGG | 0.462141 | 7.4:+32126597 | None:intergenic |
| AGTATTGATACCAGCATACC+AGG | 0.467026 | 7.4:+32126683 | None:intergenic |
| TGAGTCCTCAACTACTCAAA+TGG | 0.469573 | 7.4:-32126957 | MS.gene87054:CDS |
| TTGTGACATCTCGAAGTTGT+TGG | 0.472854 | 7.4:+32127410 | None:intergenic |
| CTCATCCATTTGAGTAGTTG+AGG | 0.479517 | 7.4:+32126952 | None:intergenic |
| AAAGAATAGCCCTGGTATGC+TGG | 0.482593 | 7.4:-32126693 | MS.gene87054:CDS |
| GACTTGATGTACTTGATGCT+AGG | 0.505174 | 7.4:-32126640 | MS.gene87054:CDS |
| ACTTGATGTACTTGATGCTA+GGG | 0.510074 | 7.4:-32126639 | MS.gene87054:CDS |
| CACTTTCCAGCTAGAAGCTG+TGG | 0.521335 | 7.4:-32126603 | MS.gene87054:CDS |
| GGAGGCGTCCACAATAATTG+AGG | 0.529677 | 7.4:+32127039 | None:intergenic |
| TCAAATGGATGAGCAACCTA+TGG | 0.542120 | 7.4:-32126942 | MS.gene87054:intron |
| AGTGTGAAAACCCTAGAAAA+GGG | 0.544492 | 7.4:-32126740 | MS.gene87054:CDS |
| GAACAAAGCCTCAATTATTG+TGG | 0.549027 | 7.4:-32127047 | MS.gene87054:CDS |
| CTCAAAAGATCAAAGAAGAG+TGG | 0.617358 | 7.4:-32127437 | MS.gene87054:CDS |
| CGATAGTATCAATGCGCAAG+TGG | 0.619080 | 7.4:-32125316 | MS.gene87054:CDS |
| ACTTTCCAGCTAGAAGCTGT+GGG | 0.644780 | 7.4:-32126602 | MS.gene87054:CDS |
| TTCCAGCTAGAAGCTGTGGG+AGG | 0.678404 | 7.4:-32126599 | MS.gene87054:CDS |
| GTATTGATACCAGCATACCA+GGG | 0.698292 | 7.4:+32126684 | None:intergenic |
| GTTGCAAGCAATCAAGAACA+CGG | 0.713497 | 7.4:-32125280 | MS.gene87054:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACATAAAAAAAAAAAGTTAA+GGG | - | chr7.4:32125405-32125424 | MS.gene87054:intron | 10.0% |
| !! | ATATATATATATATATGATG+AGG | - | chr7.4:32127311-32127330 | MS.gene87054:intron | 10.0% |
| !! | CATTTAAATCAAATTATAAA+TGG | + | chr7.4:32127043-32127062 | None:intergenic | 10.0% |
| !! | TACATAAAAAAAAAAAGTTA+AGG | - | chr7.4:32125404-32125423 | MS.gene87054:intron | 10.0% |
| !!! | GTATTTTATTTTATTTTTGT+AGG | - | chr7.4:32125943-32125962 | MS.gene87054:intron | 10.0% |
| !!! | TTTAATACTTTGTTTTTTAT+TGG | - | chr7.4:32126760-32126779 | MS.gene87054:intron | 10.0% |
| !! | AAATATTTGGTTATACAATT+TGG | - | chr7.4:32126471-32126490 | MS.gene87054:intron | 15.0% |
| !! | AATATTTGGTTATACAATTT+GGG | - | chr7.4:32126472-32126491 | MS.gene87054:intron | 15.0% |
| !! | TCCTTTAATTAATTTCAAAT+GGG | - | chr7.4:32125626-32125645 | MS.gene87054:intron | 15.0% |
| !!! | GCTTTATAAATAATTTTAAG+TGG | - | chr7.4:32126197-32126216 | MS.gene87054:intron | 15.0% |
| !!! | TAAGTATTTTTCATGAATAT+TGG | - | chr7.4:32126558-32126577 | MS.gene87054:intron | 15.0% |
| !!! | TCTAATTTTGATTTTTTCTA+AGG | - | chr7.4:32127142-32127161 | MS.gene87054:intron | 15.0% |
| !!! | TTTTTTTTTTTTTTTAGGGA+TGG | - | chr7.4:32126444-32126463 | MS.gene87054:intron | 15.0% |
| !! | ATAAAGCAATAAAGAGATTA+AGG | + | chr7.4:32126184-32126203 | None:intergenic | 20.0% |
| !! | ATATAAAAATAGAGTACAAG+AGG | + | chr7.4:32125840-32125859 | None:intergenic | 20.0% |
| !! | ATTAATTTCAAATGGGTAAT+TGG | - | chr7.4:32125633-32125652 | MS.gene87054:intron | 20.0% |
| !! | ATTATCTAGTATATTCACTT+TGG | - | chr7.4:32125505-32125524 | MS.gene87054:intron | 20.0% |
| !! | ATTATTATGTTCTTGTCTAA+AGG | - | chr7.4:32127268-32127287 | MS.gene87054:intron | 20.0% |
| !! | CTACTAACAAACAAAAATTT+GGG | - | chr7.4:32126995-32127014 | MS.gene87054:CDS | 20.0% |
| !! | CTCCTTTAATTAATTTCAAA+TGG | - | chr7.4:32125625-32125644 | MS.gene87054:intron | 20.0% |
| !! | TAGTAGAAAATATTGAACTA+TGG | - | chr7.4:32125600-32125619 | MS.gene87054:intron | 20.0% |
| !! | TCTACTAACAAACAAAAATT+TGG | - | chr7.4:32126994-32127013 | MS.gene87054:CDS | 20.0% |
| !! | TTAAAACCAATATAAAGAAG+AGG | - | chr7.4:32126254-32126273 | MS.gene87054:intron | 20.0% |
| !! | TTAATTCAATTTGAGTAAGA+TGG | - | chr7.4:32126970-32126989 | MS.gene87054:CDS | 20.0% |
| !!! | ACCCATTTGAAATTAATTAA+AGG | + | chr7.4:32125630-32125649 | None:intergenic | 20.0% |
| !!! | ATACCTATTTTTCTTTCATT+CGG | - | chr7.4:32127181-32127200 | MS.gene87054:intron | 20.0% |
| !!! | CATTTGAAATTAATTAAAGG+AGG | + | chr7.4:32125627-32125646 | None:intergenic | 20.0% |
| !!! | CTCTTCTTTTATATATAACT+TGG | - | chr7.4:32125810-32125829 | MS.gene87054:intron | 20.0% |
| ! | AAGTATATATACTTACCAAG+AGG | - | chr7.4:32126706-32126725 | MS.gene87054:CDS | 25.0% |
| ! | AATATTGAAGAAGTCTTTCA+TGG | - | chr7.4:32126414-32126433 | MS.gene87054:intron | 25.0% |
| ! | ATTCCGAATGAAAGAAAAAT+AGG | + | chr7.4:32127187-32127206 | None:intergenic | 25.0% |
| ! | GTGAGTGTATATATATGTTA+AGG | - | chr7.4:32126302-32126321 | MS.gene87054:intron | 25.0% |
| ! | TAAGAACCTCTTCTTTATAT+TGG | + | chr7.4:32126263-32126282 | None:intergenic | 25.0% |
| ! | TAATAACATTGTGTCTTCAT+TGG | + | chr7.4:32125548-32125567 | None:intergenic | 25.0% |
| ! | TTATTATCAGTCATTTCAAG+TGG | + | chr7.4:32126621-32126640 | None:intergenic | 25.0% |
| ! | TTCAATATTTATATACCCGA+TGG | + | chr7.4:32126403-32126422 | None:intergenic | 25.0% |
| !! | AAAGGAGTATATAATTCCAT+CGG | - | chr7.4:32126384-32126403 | MS.gene87054:intron | 25.0% |
| !! | TGAGAAAATCTTAATGCTTT+TGG | - | chr7.4:32126356-32126375 | MS.gene87054:intron | 25.0% |
| !! | TTTCATGATTAATGTGCTTT+TGG | - | chr7.4:32125991-32126010 | MS.gene87054:intron | 25.0% |
| !!! | TTATTTTATGCAGAGTCATA+AGG | - | chr7.4:32127368-32127387 | MS.gene87054:intron | 25.0% |
| AAATATTGAGCTCTTCGTTT+AGG | + | chr7.4:32125470-32125489 | None:intergenic | 30.0% | |
| AGTGTTATATATACTCCTCT+TGG | + | chr7.4:32126724-32126743 | None:intergenic | 30.0% | |
| ATCTAAATACGCAAAAGATG+TGG | + | chr7.4:32125901-32125920 | None:intergenic | 30.0% | |
| CTTCAACTCTTCTATGTATT+TGG | + | chr7.4:32125693-32125712 | None:intergenic | 30.0% | |
| ! | AAGGAGTATATAATTCCATC+GGG | - | chr7.4:32126385-32126404 | MS.gene87054:intron | 30.0% |
| ! | AATCATGAAACCCTTTTCTA+GGG | + | chr7.4:32125981-32126000 | None:intergenic | 30.0% |
| ! | TAATCATGAAACCCTTTTCT+AGG | + | chr7.4:32125982-32126001 | None:intergenic | 30.0% |
| !! | GTTGGCTAGTTTATTATACA+AGG | + | chr7.4:32127224-32127243 | None:intergenic | 30.0% |
| ACTTGATGTACTTGATGCTA+GGG | - | chr7.4:32126069-32126088 | MS.gene87054:intron | 35.0% | |
| AGAAGAGTTGAAGCAAAAAG+TGG | - | chr7.4:32125700-32125719 | MS.gene87054:intron | 35.0% | |
| AGTGTGAAAACCCTAGAAAA+GGG | - | chr7.4:32125968-32125987 | MS.gene87054:intron | 35.0% | |
| ATATTCCAACCCAATAAAGG+AGG | - | chr7.4:32125428-32125447 | MS.gene87054:intron | 35.0% | |
| CAACTCTTCTATGTATTTGG+AGG | + | chr7.4:32125690-32125709 | None:intergenic | 35.0% | |
| CTCAAAAGATCAAAGAAGAG+TGG | - | chr7.4:32125271-32125290 | MS.gene87054:CDS | 35.0% | |
| GAACAAAGCCTCAATTATTG+TGG | - | chr7.4:32125661-32125680 | MS.gene87054:intron | 35.0% | |
| TAGGGATGGTCACAAATATT+TGG | - | chr7.4:32126458-32126477 | MS.gene87054:intron | 35.0% | |
| TCAACAAGAATCACATGAGA+GGG | + | chr7.4:32127345-32127364 | None:intergenic | 35.0% | |
| TTCAACAAGAATCACATGAG+AGG | + | chr7.4:32127346-32127365 | None:intergenic | 35.0% | |
| ! | ATGCAAGCTCTTTCTTTTGT+TGG | + | chr7.4:32127242-32127261 | None:intergenic | 35.0% |
| ! | ATGGGAAAAGGGTATACTAA+TGG | + | chr7.4:32126589-32126608 | None:intergenic | 35.0% |
| ! | ATTAGTATACCCTTTTCCCA+TGG | - | chr7.4:32126588-32126607 | MS.gene87054:intron | 35.0% |
| ! | CAAGTAATCACGTGAGTTTT+AGG | + | chr7.4:32126949-32126968 | None:intergenic | 35.0% |
| ! | GAGAAATAGAGTACTACCAT+AGG | + | chr7.4:32125785-32125804 | None:intergenic | 35.0% |
| ! | TCTTCGTTTAGGTTATATGC+TGG | + | chr7.4:32125459-32125478 | None:intergenic | 35.0% |
| ! | TGGGAAAAGGGTATACTAAT+GGG | + | chr7.4:32126588-32126607 | None:intergenic | 35.0% |
| !!! | TTAATGCTTTTGGAGCTCAA+AGG | - | chr7.4:32126366-32126385 | MS.gene87054:intron | 35.0% |
| AATCACATGAGAGGGGAATT+TGG | + | chr7.4:32127337-32127356 | None:intergenic | 40.0% | |
| ACAAGCTCAACTGCTGTAAT+TGG | - | chr7.4:32125317-32125336 | MS.gene87054:CDS | 40.0% | |
| AGAGTTGAAGCAAAAAGTGG+AGG | - | chr7.4:32125703-32125722 | MS.gene87054:intron | 40.0% | |
| CAACAAGAATCACATGAGAG+GGG | + | chr7.4:32127344-32127363 | None:intergenic | 40.0% | |
| CTCATCCATTTGAGTAGTTG+AGG | + | chr7.4:32125759-32125778 | None:intergenic | 40.0% | |
| GACTTGATGTACTTGATGCT+AGG | - | chr7.4:32126068-32126087 | MS.gene87054:intron | 40.0% | |
| GAGTGTGAAAACCCTAGAAA+AGG | - | chr7.4:32125967-32125986 | MS.gene87054:intron | 40.0% | |
| GAGTTGAAGCAAAAAGTGGA+GGG | - | chr7.4:32125704-32125723 | MS.gene87054:intron | 40.0% | |
| GGGATATTCCAACCCAATAA+AGG | - | chr7.4:32125425-32125444 | MS.gene87054:intron | 40.0% | |
| GTTGCAAGCAATCAAGAACA+CGG | - | chr7.4:32127428-32127447 | MS.gene87054:CDS | 40.0% | |
| TCAAATGGATGAGCAACCTA+TGG | - | chr7.4:32125766-32125785 | MS.gene87054:intron | 40.0% | |
| TGAGTCCTCAACTACTCAAA+TGG | - | chr7.4:32125751-32125770 | MS.gene87054:intron | 40.0% | |
| TGGTATGCTGGTATCAATAC+TGG | - | chr7.4:32126027-32126046 | MS.gene87054:intron | 40.0% | |
| TTGTGACATCTCGAAGTTGT+TGG | + | chr7.4:32125301-32125320 | None:intergenic | 40.0% | |
| ! | GTATTGATACCAGCATACCA+GGG | + | chr7.4:32126027-32126046 | None:intergenic | 40.0% |
| ! | TTCAAGTGGTTTGTCACCAT+GGG | + | chr7.4:32126607-32126626 | None:intergenic | 40.0% |
| ! | TTTCAAGTGGTTTGTCACCA+TGG | + | chr7.4:32126608-32126627 | None:intergenic | 40.0% |
| !! | ACTGGAAGCTTTTGAAGATC+TGG | - | chr7.4:32126045-32126064 | MS.gene87054:intron | 40.0% |
| !! | AGTATTGATACCAGCATACC+AGG | + | chr7.4:32126028-32126047 | None:intergenic | 40.0% |
| !! | CTGGAAGCTTTTGAAGATCT+GGG | - | chr7.4:32126046-32126065 | MS.gene87054:intron | 40.0% |
| !! | CTTTTGGAAAAGAATAGCCC+TGG | - | chr7.4:32126007-32126026 | MS.gene87054:intron | 40.0% |
| !! | GTGTGAGCGTTGTACAATTT+TGG | - | chr7.4:32126333-32126352 | MS.gene87054:intron | 40.0% |
| AAAGAATAGCCCTGGTATGC+TGG | - | chr7.4:32126015-32126034 | MS.gene87054:intron | 45.0% | |
| ACTTTCCAGCTAGAAGCTGT+GGG | - | chr7.4:32126106-32126125 | MS.gene87054:intron | 45.0% | |
| CGATAGTATCAATGCGCAAG+TGG | - | chr7.4:32127392-32127411 | MS.gene87054:intron | 45.0% | |
| CTGGCTTCTCCTCCTTTATT+GGG | + | chr7.4:32125440-32125459 | None:intergenic | 45.0% | |
| CTTCTCCTCCTTTATTGGGT+TGG | + | chr7.4:32125436-32125455 | None:intergenic | 45.0% | |
| GAGGGAATGAACTCTGAGTT+AGG | - | chr7.4:32125722-32125741 | MS.gene87054:intron | 45.0% | |
| GGTTTGTCACCATGGGAAAA+GGG | + | chr7.4:32126600-32126619 | None:intergenic | 45.0% | |
| ! | TGGTTTGTCACCATGGGAAA+AGG | + | chr7.4:32126601-32126620 | None:intergenic | 45.0% |
| !!! | ACGTGAGTTTTAGGTGCTCA+AGG | + | chr7.4:32126940-32126959 | None:intergenic | 45.0% |
| !!! | CGTGAGTTTTAGGTGCTCAA+GGG | + | chr7.4:32126939-32126958 | None:intergenic | 45.0% |
| !! | AATAAATAAAATTTAAAAAG+AGG | + | chr7.4:32126905-32126924 | None:intergenic | 5.0% |
| !!! | ACAATTTTTTTTTTTTTTTT+AGG | - | chr7.4:32126439-32126458 | MS.gene87054:intron | 5.0% |
| !!! | CAATTTTTTTTTTTTTTTTA+GGG | - | chr7.4:32126440-32126459 | MS.gene87054:intron | 5.0% |
| !!! | TCTTTTTAAATTTTATTTAT+TGG | - | chr7.4:32126904-32126923 | MS.gene87054:intron | 5.0% |
| CACTTTCCAGCTAGAAGCTG+TGG | - | chr7.4:32126105-32126124 | MS.gene87054:intron | 50.0% | |
| ! | GCTGGCTTCTCCTCCTTTAT+TGG | + | chr7.4:32125441-32125460 | None:intergenic | 50.0% |
| !! | GGAGGCGTCCACAATAATTG+AGG | + | chr7.4:32125672-32125691 | None:intergenic | 50.0% |
| TTCCAGCTAGAAGCTGTGGG+AGG | - | chr7.4:32126109-32126128 | MS.gene87054:intron | 55.0% | |
| CTCCTCCCACAGCTTCTAGC+TGG | + | chr7.4:32126114-32126133 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 32125266 | 32127464 | 32125266 | ID=MS.gene87054 |
| chr7.4 | mRNA | 32125266 | 32127464 | 32125266 | ID=MS.gene87054.t1;Parent=MS.gene87054 |
| chr7.4 | exon | 32127399 | 32127464 | 32127399 | ID=MS.gene87054.t1.exon1;Parent=MS.gene87054.t1 |
| chr7.4 | CDS | 32127399 | 32127464 | 32127399 | ID=cds.MS.gene87054.t1;Parent=MS.gene87054.t1 |
| chr7.4 | exon | 32126943 | 32127071 | 32126943 | ID=MS.gene87054.t1.exon2;Parent=MS.gene87054.t1 |
| chr7.4 | CDS | 32126943 | 32127071 | 32126943 | ID=cds.MS.gene87054.t1;Parent=MS.gene87054.t1 |
| chr7.4 | exon | 32126595 | 32126765 | 32126595 | ID=MS.gene87054.t1.exon3;Parent=MS.gene87054.t1 |
| chr7.4 | CDS | 32126595 | 32126765 | 32126595 | ID=cds.MS.gene87054.t1;Parent=MS.gene87054.t1 |
| chr7.4 | exon | 32125266 | 32125349 | 32125266 | ID=MS.gene87054.t1.exon4;Parent=MS.gene87054.t1 |
| chr7.4 | CDS | 32125266 | 32125349 | 32125266 | ID=cds.MS.gene87054.t1;Parent=MS.gene87054.t1 |
| Gene Sequence |
| Protein sequence |
