AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63452


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63452.t1 MTR_6g085050 98.125 160 3 0 1 160 1 160 6.49e-117 327
MS.gene63452.t1 MTR_5g054770 69.536 151 37 4 14 160 11 156 1.21e-69 207
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63452.t1 AT1G68660 74.172 151 33 4 14 160 11 159 1.92e-74 220
MS.gene63452.t1 AT1G68660 55.629 151 31 5 14 160 11 129 2.28e-46 147

Find 30 sgRNAs with CRISPR-Local

Find 109 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGCAGGTGATAAATATTT+TGG 0.167312 6.3:-79259666 MS.gene63452:intron
ATGCAAGAAGCACATATTAA+TGG 0.300616 6.3:-79258004 MS.gene63452:CDS
ACTGCGTAATGAAGCTTTCA+TGG 0.335357 6.3:+79260415 None:intergenic
TTAAGTCAAATTCAGATTCT+CGG 0.388414 6.3:+79259538 None:intergenic
GTTTCAAATATGGCAAAGAT+AGG 0.389452 6.3:-79259644 MS.gene63452:CDS
GCAATATCAACTGTCATTCC+AGG 0.427583 6.3:+79258037 None:intergenic
TGCATGCAATTGAGAGGAAA+TGG 0.433575 6.3:-79257935 MS.gene63452:CDS
GAGAATCTGAATTTGACTTA+AGG 0.436402 6.3:-79259536 MS.gene63452:intron
GAGTGGATGCATTGAGTAGT+TGG 0.453547 6.3:+79260363 None:intergenic
TGGAAAAGGTGGTGGTTTGT+TGG 0.476679 6.3:-79259595 MS.gene63452:CDS
GTCAAATTCAGATTCTCGGC+CGG 0.490666 6.3:+79259542 None:intergenic
TTGTGTAGCATTACTCTGTA+TGG 0.492187 6.3:+79258112 None:intergenic
TTCCTCTCAATTGCATGCAA+TGG 0.502233 6.3:+79257939 None:intergenic
CTACTCAATGCATCCACTCC+TGG 0.512586 6.3:-79260359 MS.gene63452:intron
TTAGGTGAAATTAGAGCAAG+TGG 0.519142 6.3:+79260386 None:intergenic
ACAATGGTAGCCAGTGGAAA+AGG 0.527330 6.3:-79259609 MS.gene63452:CDS
ACTCAACAACCACCATCAGC+TGG 0.532785 6.3:+79257893 None:intergenic
TCAAATTCAGATTCTCGGCC+GGG 0.540466 6.3:+79259543 None:intergenic
AGTTCAATTGAGCCAGCTGA+TGG 0.556313 6.3:-79257905 MS.gene63452:CDS
GATGCATTGAGTAGTTGGTT+AGG 0.562303 6.3:+79260368 None:intergenic
TGAGTTATAATTACCAGGAG+TGG 0.591813 6.3:+79260346 None:intergenic
TAGGAGTATTGTAATGACAA+TGG 0.592311 6.3:-79259625 MS.gene63452:CDS
GTAGCCAGTGGAAAAGGTGG+TGG 0.601202 6.3:-79259603 MS.gene63452:CDS
GACCATTGCATGCAATTGAG+AGG 0.614338 6.3:-79257941 MS.gene63452:CDS
ATGGTAGCCAGTGGAAAAGG+TGG 0.632586 6.3:-79259606 MS.gene63452:CDS
GTGTTGATGAAAGTGATACC+TGG 0.634232 6.3:-79258055 MS.gene63452:CDS
TGTAGCATTACTCTGTATGG+TGG 0.656579 6.3:+79258115 None:intergenic
ACATATTAATGGATTAGCAG+TGG 0.658551 6.3:-79257993 MS.gene63452:CDS
TCAATTGAGCCAGCTGATGG+TGG 0.665740 6.3:-79257902 MS.gene63452:CDS
GTAATGACAATGGTAGCCAG+TGG 0.683809 6.3:-79259615 MS.gene63452:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATGAAAAAATTATTG+TGG + chr6.3:79259545-79259564 None:intergenic 10.0%
!! AATTACTAACATTTAATTAT+AGG - chr6.3:79259518-79259537 MS.gene63452:intron 10.0%
!! CCTAAAATTATAATAATAAA+GGG - chr6.3:79258418-79258437 MS.gene63452:intron 10.0%
!! TCCTAAAATTATAATAATAA+AGG - chr6.3:79258417-79258436 MS.gene63452:intron 10.0%
!!! ACTAGTAAATTATTTTATTA+GGG - chr6.3:79259265-79259284 MS.gene63452:intron 10.0%
!! ATAGATTAAATATCAAAGAT+AGG - chr6.3:79258484-79258503 MS.gene63452:intron 15.0%
!! CGTTAAAAAAAATTGTATTA+AGG - chr6.3:79258586-79258605 MS.gene63452:intron 15.0%
!!! AAATATTTTGGTTTCAAATA+TGG - chr6.3:79258654-79258673 MS.gene63452:intron 15.0%
!!! CACTAGTAAATTATTTTATT+AGG - chr6.3:79259264-79259283 MS.gene63452:intron 15.0%
!!! CCCTTTATTATTATAATTTT+AGG + chr6.3:79258421-79258440 None:intergenic 15.0%
!! AAAAAATATGGTCACTAAAT+TGG - chr6.3:79259963-79259982 MS.gene63452:intron 20.0%
!! AATTAATCAACAAAGCAAAA+TGG + chr6.3:79258064-79258083 None:intergenic 20.0%
!! ACAAACCGATAAAAAAAATA+TGG - chr6.3:79259951-79259970 MS.gene63452:intron 20.0%
!! ACAAGGAAATACAAATAAAA+GGG + chr6.3:79259720-79259739 None:intergenic 20.0%
!! ATTGTTTCAGTATATAAACT+CGG - chr6.3:79258269-79258288 MS.gene63452:intron 20.0%
!! CATATAAAATTTGTAATTGC+AGG - chr6.3:79258627-79258646 MS.gene63452:intron 20.0%
!!! AAATTTGAAGCTGTTTTTTT+AGG + chr6.3:79259193-79259212 None:intergenic 20.0%
!!! AAGTTAAGAAAATCATTTTG+TGG - chr6.3:79259659-79259678 MS.gene63452:CDS 20.0%
!!! AATATTTTCTTCAATTGAGT+TGG + chr6.3:79258344-79258363 None:intergenic 20.0%
!!! AGTGACCATATTTTTTTTAT+CGG + chr6.3:79259959-79259978 None:intergenic 20.0%
!!! CAAAATTTTGATGTGAAATA+TGG + chr6.3:79258941-79258960 None:intergenic 20.0%
!!! GTTTTATTGTTTTTGTTACT+AGG - chr6.3:79260152-79260171 MS.gene63452:intron 20.0%
! AATAATCATCTAGTTGTTAG+AGG - chr6.3:79258115-79258134 MS.gene63452:CDS 25.0%
! ACAATAAAACATGTCATCAA+AGG + chr6.3:79260142-79260161 None:intergenic 25.0%
! ACATAATTAGTTGTCAAAAG+TGG - chr6.3:79259578-79259597 MS.gene63452:CDS 25.0%
! ACATTAGAAGTTGAAAGATT+AGG - chr6.3:79258989-79259008 MS.gene63452:intron 25.0%
! ACGTAATCACATTAATTGAA+AGG - chr6.3:79259749-79259768 MS.gene63452:intron 25.0%
! ACTGAACTTCAAATTATACA+AGG + chr6.3:79258148-79258167 None:intergenic 25.0%
! AGAGATCGAAAATATCAAAT+CGG - chr6.3:79259845-79259864 MS.gene63452:intron 25.0%
! ATGTGAAATATGGTATCAAT+TGG + chr6.3:79258931-79258950 None:intergenic 25.0%
! ATTGATGAAATTGTGTAAGA+AGG - chr6.3:79258361-79258380 MS.gene63452:intron 25.0%
! CATTAGAAGTTGAAAGATTA+GGG - chr6.3:79258990-79259009 MS.gene63452:intron 25.0%
! CATTGTTTATGTAGTTACAT+AGG + chr6.3:79259800-79259819 None:intergenic 25.0%
! CTGAACTTCAAATTATACAA+GGG + chr6.3:79258147-79258166 None:intergenic 25.0%
! GACAAGGAAATACAAATAAA+AGG + chr6.3:79259721-79259740 None:intergenic 25.0%
! TAATGTTATCGTTAGAGTAA+TGG + chr6.3:79258975-79258994 None:intergenic 25.0%
! TCGTTAACATTAGAAATTAG+CGG - chr6.3:79259880-79259899 MS.gene63452:intron 25.0%
! TGATTAACGAGCTTAATTTA+TGG + chr6.3:79258314-79258333 None:intergenic 25.0%
! TTAAGTCAAATTCAGATTCT+CGG + chr6.3:79258773-79258792 None:intergenic 25.0%
! TTGTTTCAGTATATAAACTC+GGG - chr6.3:79258270-79258289 MS.gene63452:intron 25.0%
! TTTCAACATTGTTATACACA+TGG + chr6.3:79259478-79259497 None:intergenic 25.0%
!! ATTGCAGGTGATAAATATTT+TGG - chr6.3:79258642-79258661 MS.gene63452:intron 25.0%
!! TAACTGAAATACATTTTCAC+TGG + chr6.3:79258532-79258551 None:intergenic 25.0%
!!! TAGGAGTATTATAATGACTA+TGG - chr6.3:79258503-79258522 MS.gene63452:intron 25.0%
AAAACAAGCAATAAAGAGTG+TGG + chr6.3:79257989-79258008 None:intergenic 30.0%
AGTACACCTCAAATTGTAAA+AGG + chr6.3:79259237-79259256 None:intergenic 30.0%
CACAAGCATTAATATCTTGT+CGG + chr6.3:79260040-79260059 None:intergenic 30.0%
GGTCTAACTAAAATTTGACT+CGG - chr6.3:79259066-79259085 MS.gene63452:intron 30.0%
GTTCAATGGGAAGAATATAA+TGG + chr6.3:79259327-79259346 None:intergenic 30.0%
GTTTCAAATATGGCAAAGAT+AGG - chr6.3:79258664-79258683 MS.gene63452:intron 30.0%
TCAGTCGAGTTAATTAAAGT+CGG - chr6.3:79258163-79258182 MS.gene63452:intron 30.0%
TTTAACGACATTTCCTAAGT+CGG - chr6.3:79259903-79259922 MS.gene63452:intron 30.0%
! ACAATTTGAGGTGTACTTAA+GGG - chr6.3:79259240-79259259 MS.gene63452:intron 30.0%
! ACTTCACATGTTTAGTTCAA+TGG + chr6.3:79259341-79259360 None:intergenic 30.0%
! ATGCAAGAAGCACATATTAA+TGG - chr6.3:79260304-79260323 MS.gene63452:intron 30.0%
! CTTCACATGTTTAGTTCAAT+GGG + chr6.3:79259340-79259359 None:intergenic 30.0%
! GAGAATCTGAATTTGACTTA+AGG - chr6.3:79258772-79258791 MS.gene63452:intron 30.0%
! TACAATTTGAGGTGTACTTA+AGG - chr6.3:79259239-79259258 MS.gene63452:intron 30.0%
!! ACATATTAATGGATTAGCAG+TGG - chr6.3:79260315-79260334 MS.gene63452:intron 30.0%
!! TAGGAGTATTGTAATGACAA+TGG - chr6.3:79258683-79258702 MS.gene63452:intron 30.0%
!!! ATTTCTATTTTTCAAGCCAG+TGG - chr6.3:79260072-79260091 MS.gene63452:intron 30.0%
ACACATGCAGAAAGATAGAT+AGG + chr6.3:79259694-79259713 None:intergenic 35.0%
ACCATTGAAAAAACAACACC+CGG - chr6.3:79258747-79258766 MS.gene63452:intron 35.0%
ATTGTTATACACATGGAGGA+TGG + chr6.3:79259471-79259490 None:intergenic 35.0%
CAACATTGTTATACACATGG+AGG + chr6.3:79259475-79259494 None:intergenic 35.0%
CACAACTATTATAGCAGACA+AGG + chr6.3:79259419-79259438 None:intergenic 35.0%
CCAATGTAATGCTTTCATGT+TGG + chr6.3:79259048-79259067 None:intergenic 35.0%
CTTGTCTGCTATAATAGTTG+TGG - chr6.3:79259417-79259436 MS.gene63452:intron 35.0%
GATTACGTGATATCATGACA+AGG + chr6.3:79259737-79259756 None:intergenic 35.0%
GTGGATGAGTTATAATTACC+AGG + chr6.3:79257970-79257989 None:intergenic 35.0%
TGAGTTATAATTACCAGGAG+TGG + chr6.3:79257965-79257984 None:intergenic 35.0%
TTAGGTGAAATTAGAGCAAG+TGG + chr6.3:79257925-79257944 None:intergenic 35.0%
TTGTGTAGCATTACTCTGTA+TGG + chr6.3:79260199-79260218 None:intergenic 35.0%
! CTGCATCCTTTTACAATTTG+AGG - chr6.3:79259228-79259247 MS.gene63452:intron 35.0%
!! CCAACATGAAAGCATTACAT+TGG - chr6.3:79259045-79259064 MS.gene63452:intron 35.0%
!! TTTGACTCGGAGAATCTTTA+AGG - chr6.3:79259079-79259098 MS.gene63452:intron 35.0%
!!! AGGTTTTGAACTATGATGCA+TGG + chr6.3:79260122-79260141 None:intergenic 35.0%
!!! GGTGTTGTTTTTTCAATGGT+TGG + chr6.3:79258747-79258766 None:intergenic 35.0%
!!! GTTGTTTTTTCAATGGTTGG+AGG + chr6.3:79258744-79258763 None:intergenic 35.0%
ACTAACTTAGAGTCCGACTT+AGG + chr6.3:79259919-79259938 None:intergenic 40.0%
ATGTCCGTCTAAGAACCTAA+CGG + chr6.3:79259139-79259158 None:intergenic 40.0%
GATGCATTGAGTAGTTGGTT+AGG + chr6.3:79257943-79257962 None:intergenic 40.0%
GCAATATCAACTGTCATTCC+AGG + chr6.3:79260274-79260293 None:intergenic 40.0%
GCTATAATAGTTGTGGTCAC+TGG - chr6.3:79259424-79259443 MS.gene63452:intron 40.0%
TGCATGCAATTGAGAGGAAA+TGG - chr6.3:79260373-79260392 MS.gene63452:CDS 40.0%
TGTAGCATTACTCTGTATGG+TGG + chr6.3:79260196-79260215 None:intergenic 40.0%
TTCCTCTCAATTGCATGCAA+TGG + chr6.3:79260372-79260391 None:intergenic 40.0%
! GTGTTGATGAAAGTGATACC+TGG - chr6.3:79260253-79260272 MS.gene63452:intron 40.0%
!! CGGAGAATCTTTAAGGTCTT+GGG - chr6.3:79259086-79259105 MS.gene63452:intron 40.0%
!! TCGGAGAATCTTTAAGGTCT+TGG - chr6.3:79259085-79259104 MS.gene63452:intron 40.0%
ACAATGGTAGCCAGTGGAAA+AGG - chr6.3:79258699-79258718 MS.gene63452:intron 45.0%
ACGAACATCATATACGCCAC+TGG + chr6.3:79260091-79260110 None:intergenic 45.0%
AGTTCAATTGAGCCAGCTGA+TGG - chr6.3:79260403-79260422 MS.gene63452:CDS 45.0%
ATGCCCGTTAGGTTCTTAGA+CGG - chr6.3:79259132-79259151 MS.gene63452:intron 45.0%
GACCATTGCATGCAATTGAG+AGG - chr6.3:79260367-79260386 MS.gene63452:CDS 45.0%
GAGTGGATGCATTGAGTAGT+TGG + chr6.3:79257948-79257967 None:intergenic 45.0%
GGATAGAAAGAATGCCCGTT+AGG - chr6.3:79259121-79259140 MS.gene63452:intron 45.0%
GTAATGACAATGGTAGCCAG+TGG - chr6.3:79258693-79258712 MS.gene63452:intron 45.0%
GTCAAATTCAGATTCTCGGC+CGG + chr6.3:79258769-79258788 None:intergenic 45.0%
TCAAATTCAGATTCTCGGCC+GGG + chr6.3:79258768-79258787 None:intergenic 45.0%
TGTCCGTCTAAGAACCTAAC+GGG + chr6.3:79259138-79259157 None:intergenic 45.0%
! TGGAAAAGGTGGTGGTTTGT+TGG - chr6.3:79258713-79258732 MS.gene63452:intron 45.0%
!!! GCCGGGTGTTGTTTTTTCAA+TGG + chr6.3:79258751-79258770 None:intergenic 45.0%
ATGGTAGCCAGTGGAAAAGG+TGG - chr6.3:79258702-79258721 MS.gene63452:intron 50.0%
CTACTCAATGCATCCACTCC+TGG - chr6.3:79257949-79257968 MS.gene63452:CDS 50.0%
GGTCTTGGGTCTAAGTCTTG+TGG - chr6.3:79259100-79259119 MS.gene63452:intron 50.0%
TCAATTGAGCCAGCTGATGG+TGG - chr6.3:79260406-79260425 MS.gene63452:CDS 50.0%
! CAAACCACCACCTTTTCCAC+TGG + chr6.3:79258712-79258731 None:intergenic 50.0%
GTAGCCAGTGGAAAAGGTGG+TGG - chr6.3:79258705-79258724 MS.gene63452:intron 55.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 79257895 79260435 79257895 ID=MS.gene63452
chr6.3 mRNA 79257895 79260435 79257895 ID=MS.gene63452.t1;Parent=MS.gene63452
chr6.3 exon 79260360 79260435 79260360 ID=MS.gene63452.t1.exon1;Parent=MS.gene63452.t1
chr6.3 CDS 79260360 79260435 79260360 ID=cds.MS.gene63452.t1;Parent=MS.gene63452.t1
chr6.3 exon 79259537 79259681 79259537 ID=MS.gene63452.t1.exon2;Parent=MS.gene63452.t1
chr6.3 CDS 79259537 79259681 79259537 ID=cds.MS.gene63452.t1;Parent=MS.gene63452.t1
chr6.3 exon 79257895 79258156 79257895 ID=MS.gene63452.t1.exon3;Parent=MS.gene63452.t1
chr6.3 CDS 79257895 79258156 79257895 ID=cds.MS.gene63452.t1;Parent=MS.gene63452.t1
Gene Sequence

>MS.gene63452

ATGAAAGCTTCATTACGCAGTGCTACTCCACTTGCTCTAATTTCACCTAACCAACTACTCAATGCATCCACTCCTGGTAATTATAACTCATCCACACTCTTTATTGCTTGTTTTCACTTTCTTGAATTCAAACATATTATTTTACTTATGATTATCATTTTTAAAACCATTTTGCTTTGTTGATTAATTTCACAAACATTACAAGTTTTATATTTTCTTTAATAATCATCTAGTTGTTAGAGGAAAAGTCCCTTGTATAATTTGAAGTTCAGTCGAGTTAATTAAAGTCGGACTTTAATAATCATCTAGTTGTTAGAGGAAAAGTCCCTTGTATAATTTGAAGTTCAGTCGAGTTAATTAAAGTCGGACTAAAGATTGTTTCAGTATATAAACTCGGGTTATTTCAAACACTTTGACCATAAATTAAGCTCGTTAATCAATTGAGTCCAACTCAATTGAAGAAAATATTGATGAAATTGTGTAAGAAGGATATAACTAGTTGTTTTATATTTATGATATTTTTCCTAAAATTATAATAATAAAGGGATATAGAATGTATACTCGTTAAGATATTTGAGCTCTAATTTTAATAGATTAAATATCAAAGATAGGAGTATTATAATGACTATGGTAGCCAGTGAAAATGTATTTCAGTTAAGACATTTCAGCTCTAATTTTAATAGACATTTCTCGTTAAAAAAAATTGTATTAAGGTTCAAATGTTAATTGATACATATAAAATTTGTAATTGCAGGTGATAAATATTTTGGTTTCAAATATGGCAAAGATAGGAGTATTGTAATGACAATGGTAGCCAGTGGAAAAGGTGGTGGTTTGTTGGACAAGCCTCCAACCATTGAAAAAACAACACCCGGCCGAGAATCTGAATTTGACTTAAGGTAATACGAAAAAAGTTATCTTATTTGAACATCAAAATACAGAATGTGTGATACTGTATATATTTATCTTTATTTTTTTTTTCTTACTTTTTATCTTATGTAACTTACACTCATATCAAAAGTATGCATAAATACCAATTGATACCATATTTCACATCAAAATTTTGATGTCAAAGTACCATTACTCTAACGATAACATTAGAAGTTGAAAGATTAGGGTAGTAAACATTACTTTAAATCGTCTCAAATTACCAACATGAAAGCATTACATTGGTCTAACTAAAATTTGACTCGGAGAATCTTTAAGGTCTTGGGTCTAAGTCTTGTGGATAGAAAGAATGCCCGTTAGGTTCTTAGACGGACATTAATCATCGACAAGCTAATAGATACTTCACACCTAAAAAAACAGCTTCAAATTTTAAATTCAAATGTTGCTGCATCCTTTTACAATTTGAGGTGTACTTAAGGGACACTAGTAAATTATTTTATTAGGGACTTATATTGATAACATGAGCTATATAGTTCACATTCCATTATATTCTTCCCATTGAACTAAACATGTGAAGTTATTAGAACTGATTAAAGTAGATTGAAAAACATGTTGATCTTTATATTCAAGTTTCCTTGTCTGCTATAATAGTTGTGGTCACTGGTCATTTCAATTGCTAATGATTCCATCCTCCATGTGTATAACAATGTTGAAAATCAGTCTTTGTGCTTTCACAATTACTAACATTTAATTATAGGTCCACAATAATTTTTTCATTTTTTTAGATAACTAAAGACATAATTAGTTGTCAAAAGTGGATATATGAATGAATGATTTCTTTTACTTCATGTGTCTTTGAAAAGAAGTGTAACTATGAAGTTAAGAAAATCATTTTGTGGAAGCTCATTCCTATCTATCTTTCTGCATGTGTAGTCCCTTTTATTTGTATTTCCTTGTCATGATATCACGTAATCACATTAATTGAAAGGCAAAATGAGTAGTCAAAACTGAAAACCTATGTAACTACATAAACAATGTCATGTCATATATAGCGAGCGATTTAGAGATCGAAAATATCAAATCGGTCGTTAAATCGATCGTTAACATTAGAAATTAGCGGTTTAACGACATTTCCTAAGTCGGACTCTAAGTTAGTCAAAAATCATTTACAAACCGATAAAAAAAATATGGTCACTAAATTGGTCGACTAATAGCGATTTTTTAGTACTTCAGATTGAAGACATGTTTCAGTGTCCGACAAGATATTAATGCTTGTGATTATATACATCATTTCTATTTTTCAAGCCAGTGGCGTATATGATGTTCGTGTCTGTATCCATGCATCATAGTTCAAAACCTTTGATGACATGTTTTATTGTTTTTGTTACTAGGAAATCAAAAAAGACATCTCCACCATACAGAGTAATGCTACACAATGACAACTATAACAAAAGAGAATATGTTGTGCAAGTGTTGATGAAAGTGATACCTGGAATGACAGTTGATATTGCAGTTAACGTCATGCAAGAAGCACATATTAATGGATTAGCAGTGGTCATTATTTGTGCTCAAGTTGATGCTGAAGACCATTGCATGCAATTGAGAGGAAATGGTCTTTTAAGTTCAATTGAGCCAGCTGATGGTGGTTGTTGA

Protein sequence

>MS.gene63452.t1

MKASLRSATPLALISPNQLLNASTPGDKYFGFKYGKDRSIVMTMVASGKGGGLLDKPPTIEKTTPGRESEFDLRKSKKTSPPYRVMLHNDNYNKREYVVQVLMKVIPGMTVDIAVNVMQEAHINGLAVVIICAQVDAEDHCMQLRGNGLLSSIEPADGGC