AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene09795


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09795.t1 MTR_7g100540 99.213 127 1 0 1 127 125 251 3.06e-90 261
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09795.t1 AT2G37240 81.102 127 24 0 1 127 122 248 8.46e-75 223
MS.gene09795.t1 AT5G65840 32.031 128 83 3 1 126 145 270 4.23e-13 64.3

Find 33 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAAGATATCTTCAATTTC+TGG 0.106966 7.2:+11641100 None:intergenic
GCTGAGCAAACAAAATTTAA+AGG 0.173089 7.2:-11641997 MS.gene09795:CDS
GAGGCCTTACAATTTGTTTC+TGG 0.198475 7.2:-11641849 MS.gene09795:CDS
TTAAGATATCTTCAATTTCT+GGG 0.369252 7.2:+11641101 None:intergenic
TGTTTGCTCAGCAAAGGCTT+TGG 0.376817 7.2:+11642008 None:intergenic
TAAGATAATAGAATTGTATA+TGG 0.387939 7.2:-11641656 MS.gene09795:CDS
CATACACACCTTGTGAATAT+AGG 0.399241 7.2:+11641248 None:intergenic
CAAGGAGGAATTATAGTTGC+AGG 0.415807 7.2:-11641291 MS.gene09795:CDS
GGTGTAGCACTTGTATTGAT+TGG 0.434510 7.2:-11642127 MS.gene09795:CDS
TTGGAAGCTTTCATTCGAAA+AGG 0.452486 7.2:-11641617 MS.gene09795:CDS
TGGAAGGTTATCGACAAGAT+TGG 0.474176 7.2:-11641636 MS.gene09795:CDS
AACTCCAGAAACAAATTGTA+AGG 0.478715 7.2:+11641845 None:intergenic
GGAATTATAGTTGCAGGTCC+TGG 0.514996 7.2:-11641285 MS.gene09795:CDS
CCCCGCTCAATCGTCATATG+AGG 0.519619 7.2:-11641868 MS.gene09795:CDS
GCCTCATATGACGATTGAGC+GGG 0.522306 7.2:+11641867 None:intergenic
GGCTGTTTCTCTTACTTTGG+GGG 0.526708 7.2:+11641809 None:intergenic
TGGACCTGGCAACATTGATC+AGG 0.530445 7.2:-11642107 MS.gene09795:intron
ATAGTTGCAGGTCCTGGTAA+AGG 0.534747 7.2:-11641279 MS.gene09795:CDS
CAGGATATAATGGATGCGTC+TGG 0.535386 7.2:-11642148 None:intergenic
CATGAGTAGGACAAAGAAGC+AGG 0.545391 7.2:-11641130 MS.gene09795:intron
TGTACCTGATCAATGTTGCC+AGG 0.557725 7.2:+11642103 None:intergenic
ATAATAGAATTGTATATGGA+AGG 0.563362 7.2:-11641652 MS.gene09795:CDS
AAGGATACTGTTAGCAGAGG+AGG 0.583125 7.2:-11641598 MS.gene09795:CDS
TAGGAGATGTTACCTTTACC+AGG 0.585319 7.2:+11641267 None:intergenic
TGACACTGTGGCAGGCAACA+AGG 0.606308 7.2:-11641309 MS.gene09795:intron
ATACTGTTAGCAGAGGAGGC+TGG 0.626834 7.2:-11641594 MS.gene09795:intron
GCACTTGTATTGATTGGACC+TGG 0.646245 7.2:-11642121 MS.gene09795:CDS
GAGCAAACAAAATTTAAAGG+AGG 0.650865 7.2:-11641994 MS.gene09795:intron
GAAAAGGATACTGTTAGCAG+AGG 0.658204 7.2:-11641601 MS.gene09795:CDS
GGCCTCATATGACGATTGAG+CGG 0.670971 7.2:+11641866 None:intergenic
CCTCATATGACGATTGAGCG+GGG 0.684441 7.2:+11641868 None:intergenic
TAACATCTCCTATATTCACA+AGG 0.715027 7.2:-11641256 MS.gene09795:intron
CACTGTGGCAGGCAACAAGG+AGG 0.721602 7.2:-11641306 MS.gene09795:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTACTTATGAATAAAAAATT+AGG + chr7.2:11641438-11641457 None:intergenic 10.0%
!! ATTCTATAAAATTGAGTATA+GGG + chr7.2:11642006-11642025 None:intergenic 15.0%
!! TAAGATAATAGAATTGTATA+TGG - chr7.2:11641567-11641586 MS.gene09795:intron 15.0%
!! TATTCTATAAAATTGAGTAT+AGG + chr7.2:11642007-11642026 None:intergenic 15.0%
!!! AGTTTTTGTTAAATATACAA+AGG - chr7.2:11641504-11641523 MS.gene09795:intron 15.0%
!!! CAATTTTATAGAATATATCT+TGG - chr7.2:11642012-11642031 MS.gene09795:CDS 15.0%
!! AATATATCTTGGAATATTCA+TGG - chr7.2:11642023-11642042 MS.gene09795:intron 20.0%
!! AGTAATAAATAAAATCCATG+TGG - chr7.2:11641453-11641472 MS.gene09795:intron 20.0%
!! ATAATAGAATTGTATATGGA+AGG - chr7.2:11641571-11641590 MS.gene09795:intron 20.0%
!! CTAATACCTATATTTAATAC+AGG - chr7.2:11641192-11641211 MS.gene09795:intron 20.0%
!! TACATTGAATCTATAATTAG+AGG - chr7.2:11641782-11641801 MS.gene09795:intron 20.0%
!! TGTCAAGTTCATAATATTAA+AGG + chr7.2:11641899-11641918 None:intergenic 20.0%
!! TTAAGATATCTTCAATTTCT+GGG + chr7.2:11642125-11642144 None:intergenic 20.0%
!!! TTTAAGATATCTTCAATTTC+TGG + chr7.2:11642126-11642145 None:intergenic 20.0%
!! GATGTCTTCTTTATCATTTT+TGG - chr7.2:11641730-11641749 MS.gene09795:intron 25.0%
AACTCCAGAAACAAATTGTA+AGG + chr7.2:11641381-11641400 None:intergenic 30.0%
ATATTATGAACTTGACACTG+TGG - chr7.2:11641902-11641921 MS.gene09795:intron 30.0%
ATTATCCATACCACTCTTAA+AGG - chr7.2:11641698-11641717 MS.gene09795:intron 30.0%
CTTTGGCCTGTATTAAATAT+AGG + chr7.2:11641201-11641220 None:intergenic 30.0%
GAGCAAACAAAATTTAAAGG+AGG - chr7.2:11641229-11641248 MS.gene09795:intron 30.0%
GCTGAGCAAACAAAATTTAA+AGG - chr7.2:11641226-11641245 MS.gene09795:intron 30.0%
TAACATCTCCTATATTCACA+AGG - chr7.2:11641967-11641986 MS.gene09795:intron 30.0%
TGCAACAGCTAAAATTAACA+CGG + chr7.2:11641533-11641552 None:intergenic 30.0%
!! AAATTTTGTTTGCTCAGCAA+AGG + chr7.2:11641224-11641243 None:intergenic 30.0%
CAACAGCTAAAATTAACACG+GGG + chr7.2:11641531-11641550 None:intergenic 35.0%
CATACACACCTTGTGAATAT+AGG + chr7.2:11641978-11641997 None:intergenic 35.0%
GATAGTGTAAGACTTCTTGT+AGG + chr7.2:11641668-11641687 None:intergenic 35.0%
GCAACAGCTAAAATTAACAC+GGG + chr7.2:11641532-11641551 None:intergenic 35.0%
TACATTACAGAAACTAGCAC+AGG + chr7.2:11641276-11641295 None:intergenic 35.0%
TGATAAAGAAGACATCACAG+AGG + chr7.2:11641726-11641745 None:intergenic 35.0%
TGATAGAAGAAAGCTCTCTT+TGG - chr7.2:11641829-11641848 MS.gene09795:CDS 35.0%
TTGGAAGCTTTCATTCGAAA+AGG - chr7.2:11641606-11641625 MS.gene09795:CDS 35.0%
!! TAGGCTGTTTCTCTTACTTT+GGG + chr7.2:11641419-11641438 None:intergenic 35.0%
!! TCTGCATAGATTTCTAGAAC+AGG + chr7.2:11641335-11641354 None:intergenic 35.0%
!! TTAGGCTGTTTCTCTTACTT+TGG + chr7.2:11641420-11641439 None:intergenic 35.0%
!! TTCCAGCATTCTAAGATGTT+AGG + chr7.2:11641164-11641183 None:intergenic 35.0%
!!! AATTTTAGCTGTTGCACTAC+AGG - chr7.2:11641537-11641556 MS.gene09795:intron 35.0%
ATCAGCACTTTAACAGAACC+TGG - chr7.2:11641306-11641325 MS.gene09795:intron 40.0%
CAAGGAGGAATTATAGTTGC+AGG - chr7.2:11641932-11641951 MS.gene09795:intron 40.0%
CTCCTAACATCTTAGAATGC+TGG - chr7.2:11641159-11641178 MS.gene09795:intron 40.0%
CTTTCTTCTATCAGCAAGCA+AGG + chr7.2:11641822-11641841 None:intergenic 40.0%
GAAAAGGATACTGTTAGCAG+AGG - chr7.2:11641622-11641641 MS.gene09795:CDS 40.0%
TAGGAGATGTTACCTTTACC+AGG + chr7.2:11641959-11641978 None:intergenic 40.0%
TGGAAGGTTATCGACAAGAT+TGG - chr7.2:11641587-11641606 MS.gene09795:intron 40.0%
! AGGATCCTTTAAGAGTGGTA+TGG + chr7.2:11641706-11641725 None:intergenic 40.0%
! GAGGCCTTACAATTTGTTTC+TGG - chr7.2:11641374-11641393 MS.gene09795:intron 40.0%
! GGTGTAGCACTTGTATTGAT+TGG - chr7.2:11641096-11641115 MS.gene09795:CDS 40.0%
! TCTTACTTTGGGGGTAAATG+TGG + chr7.2:11641408-11641427 None:intergenic 40.0%
!! AGGCTGTTTCTCTTACTTTG+GGG + chr7.2:11641418-11641437 None:intergenic 40.0%
!! GATTTCTAGAACAGGTAACC+AGG + chr7.2:11641327-11641346 None:intergenic 40.0%
AAGGATACTGTTAGCAGAGG+AGG - chr7.2:11641625-11641644 MS.gene09795:CDS 45.0%
ATAGTTGCAGGTCCTGGTAA+AGG - chr7.2:11641944-11641963 MS.gene09795:intron 45.0%
CATGAGTAGGACAAAGAAGC+AGG - chr7.2:11642093-11642112 MS.gene09795:intron 45.0%
GCAACATTGATCAGGTACAC+GGG - chr7.2:11641124-11641143 MS.gene09795:CDS 45.0%
GGAATTATAGTTGCAGGTCC+TGG - chr7.2:11641938-11641957 MS.gene09795:intron 45.0%
GGCAACATTGATCAGGTACA+CGG - chr7.2:11641123-11641142 MS.gene09795:CDS 45.0%
TATGAACTTGACACTGTGGC+AGG - chr7.2:11641906-11641925 MS.gene09795:intron 45.0%
TCAAACGTGCTCACATGAGT+AGG - chr7.2:11642080-11642099 MS.gene09795:intron 45.0%
TGTACCTGATCAATGTTGCC+AGG + chr7.2:11641123-11641142 None:intergenic 45.0%
TGTTTGCTCAGCAAAGGCTT+TGG + chr7.2:11641218-11641237 None:intergenic 45.0%
! CACAGAGGATCCTTTAAGAG+TGG + chr7.2:11641711-11641730 None:intergenic 45.0%
!! GCACTTGTATTGATTGGACC+TGG - chr7.2:11641102-11641121 MS.gene09795:CDS 45.0%
!! GGCTGTTTCTCTTACTTTGG+GGG + chr7.2:11641417-11641436 None:intergenic 45.0%
ACACACATCACTCAGCCACA+TGG + chr7.2:11641471-11641490 None:intergenic 50.0%
ATACTGTTAGCAGAGGAGGC+TGG - chr7.2:11641629-11641648 MS.gene09795:CDS 50.0%
CCTCATATGACGATTGAGCG+GGG + chr7.2:11641358-11641377 None:intergenic 50.0%
GCCTCATATGACGATTGAGC+GGG + chr7.2:11641359-11641378 None:intergenic 50.0%
GGCCTCATATGACGATTGAG+CGG + chr7.2:11641360-11641379 None:intergenic 50.0%
TGGACCTGGCAACATTGATC+AGG - chr7.2:11641116-11641135 MS.gene09795:CDS 50.0%
TTAGCTGTTGCACTACAGGC+AGG - chr7.2:11641541-11641560 MS.gene09795:intron 50.0%
CCCCGCTCAATCGTCATATG+AGG - chr7.2:11641355-11641374 MS.gene09795:intron 55.0%
TGACACTGTGGCAGGCAACA+AGG - chr7.2:11641914-11641933 MS.gene09795:intron 55.0%
CACTGTGGCAGGCAACAAGG+AGG - chr7.2:11641917-11641936 MS.gene09795:intron 60.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 11641084 11642161 11641084 ID=MS.gene09795
chr7.2 mRNA 11641084 11642161 11641084 ID=MS.gene09795.t1;Parent=MS.gene09795
chr7.2 exon 11642108 11642161 11642108 ID=MS.gene09795.t1.exon1;Parent=MS.gene09795.t1
chr7.2 CDS 11642108 11642161 11642108 ID=cds.MS.gene09795.t1;Parent=MS.gene09795.t1
chr7.2 exon 11641995 11642031 11641995 ID=MS.gene09795.t1.exon2;Parent=MS.gene09795.t1
chr7.2 CDS 11641995 11642031 11641995 ID=cds.MS.gene09795.t1;Parent=MS.gene09795.t1
chr7.2 exon 11641824 11641903 11641824 ID=MS.gene09795.t1.exon3;Parent=MS.gene09795.t1
chr7.2 CDS 11641824 11641903 11641824 ID=cds.MS.gene09795.t1;Parent=MS.gene09795.t1
chr7.2 exon 11641595 11641686 11641595 ID=MS.gene09795.t1.exon4;Parent=MS.gene09795.t1
chr7.2 CDS 11641595 11641686 11641595 ID=cds.MS.gene09795.t1;Parent=MS.gene09795.t1
chr7.2 exon 11641257 11641317 11641257 ID=MS.gene09795.t1.exon5;Parent=MS.gene09795.t1
chr7.2 CDS 11641257 11641317 11641257 ID=cds.MS.gene09795.t1;Parent=MS.gene09795.t1
chr7.2 exon 11641084 11641143 11641084 ID=MS.gene09795.t1.exon6;Parent=MS.gene09795.t1
chr7.2 CDS 11641084 11641143 11641084 ID=cds.MS.gene09795.t1;Parent=MS.gene09795.t1
Gene Sequence

>MS.gene09795

ATGGATGCGTCTGGTGTAGCACTTGTATTGATTGGACCTGGCAACATTGATCAGGTACACGGGATTTTCTGCATTCTCCTAACATCTTAGAATGCTGGAATTTTGTAGCTAATACCTATATTTAATACAGGCCAAAGCCTTTGCTGAGCAAACAAAATTTAAAGGAGGTACTCTACAGCTGACATAATACCTGTGCTAGTTTCTGTAATGTACTCATCTTATATCAGCACTTTAACAGAACCTGGTTACCTGTTCTAGAAATCTATGCAGACCCCGCTCAATCGTCATATGAGGCCTTACAATTTGTTTCTGGAGTTTTAACCACATTTACCCCCAAAGTAAGAGAAACAGCCTAATTTTTTATTCATAAGTAATAAATAAAATCCATGTGGCTGAGTGATGTGTGTGTTTATAAATTGAAGTTTTTGTTAAATATACAAAGGACCCCGTGTTAATTTTAGCTGTTGCACTACAGGCAGGTCTTAAGATAATAGAATTGTATATGGAAGGTTATCGACAAGATTGGAAGCTTTCATTCGAAAAGGATACTGTTAGCAGAGGAGGCTGGTATGTAAATTTGTCCTACAAGAAGTCTTACACTATCACATTATCTGATTATCCATACCACTCTTAAAGGATCCTCTGTGATGTCTTCTTTATCATTTTTGGTTTGCGAAAAAATCGTAAAGATATGTGTCTACATTGAATCTATAATTAGAGGATAAGAAGTTCATTCCTTGCTTGCTGATAGAAGAAAGCTCTCTTTGGACATTTTTTATTTGTTATGTATGTGAGTTATGATCATAATTTTTCCTTTAATATTATGAACTTGACACTGTGGCAGGCAACAAGGAGGAATTATAGTTGCAGGTCCTGGTAAAGGTAACATCTCCTATATTCACAAGGTGTGTATGTCAAACCCTATACTCAATTTTATAGAATATATCTTGGAATATTCATGGTATACATGTTTAACATTTTTATGTTATCATTATGTCAAACGTGCTCACATGAGTAGGACAAAGAAGCAGGAGATGACCCAGAAATTGAAGATATCTTAAAAGCATGTTGCTCGTGA

Protein sequence

>MS.gene09795.t1

MDASGVALVLIGPGNIDQAKAFAEQTKFKGEIYADPAQSSYEALQFVSGVLTTFTPKAGLKIIELYMEGYRQDWKLSFEKDTVSRGGWQQGGIIVAGPGKGNISYIHKDKEAGDDPEIEDILKACCS