AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49929


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49929.t1 MTR_6g085050 97.500 160 4 0 1 160 1 160 9.24e-116 324
MS.gene49929.t1 MTR_5g054770 69.536 151 37 4 14 160 11 156 2.88e-70 209
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49929.t1 AT1G68660 74.172 151 33 4 14 160 11 159 1.45e-74 220
MS.gene49929.t1 AT1G68660 55.629 151 31 5 14 160 11 129 1.54e-46 148

Find 29 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTACAGGTGATAAATATTT+TGG 0.160796 6.4:-59136231 MS.gene49929:intron
ATGCAAGAAGCACATATTAA+TGG 0.300616 6.4:-59134559 MS.gene49929:CDS
ACTGCGTAATGAAGCTTTCA+TGG 0.321586 6.4:+59136870 None:intergenic
GTTTCAAACAAGGCAAAGAT+AGG 0.391817 6.4:-59136209 MS.gene49929:CDS
GCAATATCAACTGTCATTCC+AGG 0.427583 6.4:+59134592 None:intergenic
TGCATGCAATTGAGAGGAAA+TGG 0.433575 6.4:-59134490 MS.gene49929:CDS
GAGAATCTGAATTTGACTTA+AGG 0.436402 6.4:-59136101 MS.gene49929:intron
GAGTGGATGCATTGAGTAGT+TGG 0.453547 6.4:+59136818 None:intergenic
TGGAAAAGGTGGTGGTTTGT+TGG 0.476679 6.4:-59136160 MS.gene49929:CDS
TTGTGTAGCATTACTCTGTA+TGG 0.492187 6.4:+59134667 None:intergenic
GTCAAATTCAGATTCTCGAC+CGG 0.494941 6.4:+59136107 None:intergenic
TTCCTCTCAATTGCATGCAA+TGG 0.502233 6.4:+59134494 None:intergenic
CTACTCAATGCATCCACTCC+TGG 0.512586 6.4:-59136814 MS.gene49929:intron
TTAGGTGAAATTAGAGCAAG+TGG 0.519142 6.4:+59136841 None:intergenic
ACAATGGTAGCCAGTGGAAA+AGG 0.527330 6.4:-59136174 MS.gene49929:CDS
ACTCAACAACCACCATCAGC+TGG 0.532785 6.4:+59134448 None:intergenic
AGTTCAATTGAGCCAGCTGA+TGG 0.556313 6.4:-59134460 MS.gene49929:CDS
GATGCATTGAGTAGTTGGTT+AGG 0.562303 6.4:+59136823 None:intergenic
TAGGAGTATTGTAATGACAA+TGG 0.592311 6.4:-59136190 MS.gene49929:CDS
TCAAATTCAGATTCTCGACC+GGG 0.594401 6.4:+59136108 None:intergenic
GTAGCCAGTGGAAAAGGTGG+TGG 0.601202 6.4:-59136168 MS.gene49929:CDS
GACCATTGCATGCAATTGAG+AGG 0.614338 6.4:-59134496 MS.gene49929:CDS
ATGGTAGCCAGTGGAAAAGG+TGG 0.632586 6.4:-59136171 MS.gene49929:CDS
GTGTTGATGAAAGTGATACC+TGG 0.634232 6.4:-59134610 MS.gene49929:CDS
TGTGTTATAATTACCAGGAG+TGG 0.636157 6.4:+59136801 None:intergenic
TGTAGCATTACTCTGTATGG+TGG 0.656579 6.4:+59134670 None:intergenic
ACATATTAATGGATTAGCAG+TGG 0.658551 6.4:-59134548 MS.gene49929:CDS
TCAATTGAGCCAGCTGATGG+TGG 0.665740 6.4:-59134457 MS.gene49929:CDS
GTAATGACAATGGTAGCCAG+TGG 0.683809 6.4:-59136180 MS.gene49929:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATGAAAATATTATTG+TGG + chr6.4:59136001-59136020 None:intergenic 10.0%
!! AATTACTAACATTTAATAAT+AGG - chr6.4:59135974-59135993 MS.gene49929:intron 10.0%
!! CCTAAAATTATAATAATAAA+GGG - chr6.4:59134864-59134883 MS.gene49929:intron 10.0%
!! TCCTAAAATTATAATAATAA+AGG - chr6.4:59134863-59134882 MS.gene49929:intron 10.0%
!!! ACTAGTAAATTATTTTATTA+GGG - chr6.4:59135721-59135740 MS.gene49929:intron 10.0%
!! ATAGATTAAATATCAAAGAT+AGG - chr6.4:59134930-59134949 MS.gene49929:intron 15.0%
!! CATATAAAATTTGTAATTAC+AGG - chr6.4:59135072-59135091 MS.gene49929:intron 15.0%
!! TCGTTAAAAAAATTGTATTA+AGG - chr6.4:59135031-59135050 MS.gene49929:intron 15.0%
!!! CACTAGTAAATTATTTTATT+AGG - chr6.4:59135720-59135739 MS.gene49929:intron 15.0%
!!! CCCTTTATTATTATAATTTT+AGG + chr6.4:59134867-59134886 None:intergenic 15.0%
!! AATAATTATCTAGTTGTTAG+AGG - chr6.4:59134637-59134656 MS.gene49929:CDS 20.0%
!! ACATAATCACATTAATTGAA+AGG - chr6.4:59136203-59136222 MS.gene49929:CDS 20.0%
!! ATTGTTTCAGTATATAAACT+CGG - chr6.4:59134715-59134734 MS.gene49929:intron 20.0%
!! TCATTAACATTAGAAATTAG+TGG - chr6.4:59136336-59136355 MS.gene49929:intron 20.0%
!!! AAATATTTTGGTTTCAAACA+AGG - chr6.4:59135099-59135118 MS.gene49929:intron 20.0%
!!! AAATTTGAAGCTGTTTTTTT+AGG + chr6.4:59135649-59135668 None:intergenic 20.0%
!!! AATATTTTCTTCAATTGAGT+TGG + chr6.4:59134790-59134809 None:intergenic 20.0%
!!! ATTACAGGTGATAAATATTT+TGG - chr6.4:59135087-59135106 MS.gene49929:intron 20.0%
!!! CAAAATTTTGATGTGAAATA+TGG + chr6.4:59135397-59135416 None:intergenic 20.0%
!!! GTTTTATTGTTTTTGTTACT+AGG - chr6.4:59136607-59136626 MS.gene49929:intron 20.0%
! ACAAGGAAATACAAAGAAAA+GGG + chr6.4:59136174-59136193 None:intergenic 25.0%
! ACAATAAAACATGTCATCAA+AGG + chr6.4:59136597-59136616 None:intergenic 25.0%
! ACATAATTAGTTGTCAAAAG+TGG - chr6.4:59136034-59136053 MS.gene49929:intron 25.0%
! ACATTAGAAGTTGAAAGATT+AGG - chr6.4:59135445-59135464 MS.gene49929:intron 25.0%
! ACTGAACTTCAAATTATACA+AGG + chr6.4:59134670-59134689 None:intergenic 25.0%
! AGAGATCGAAAATATCAAAT+TGG - chr6.4:59136299-59136318 MS.gene49929:intron 25.0%
! ATTGATGAAATTGTGTAAGA+AGG - chr6.4:59134807-59134826 MS.gene49929:intron 25.0%
! CATTAGAAGTTGAAAGATTA+GGG - chr6.4:59135446-59135465 MS.gene49929:intron 25.0%
! CATTGTTTATGTAGTTACAT+AGG + chr6.4:59136254-59136273 None:intergenic 25.0%
! CTGAACTTCAAATTATACAA+GGG + chr6.4:59134669-59134688 None:intergenic 25.0%
! TAATGTTATCGTTAGAGTAA+TGG + chr6.4:59135431-59135450 None:intergenic 25.0%
! TAGTAACCGATAACAAAATT+TGG - chr6.4:59136406-59136425 MS.gene49929:intron 25.0%
! TGATTAACGAGCTTAATTTA+TGG + chr6.4:59134760-59134779 None:intergenic 25.0%
! TTGTTTCAGTATATAAACTC+GGG - chr6.4:59134716-59134735 MS.gene49929:intron 25.0%
! TTTCAACATTGTTATACACA+TGG + chr6.4:59135934-59135953 None:intergenic 25.0%
!! AAGTTGAGAAAATCATTTTG+TGG - chr6.4:59136113-59136132 MS.gene49929:CDS 25.0%
!! TAACTGAAATACATTTTCAC+TGG + chr6.4:59134978-59134997 None:intergenic 25.0%
!!! TAGGAGTATTATAATGACTA+TGG - chr6.4:59134949-59134968 MS.gene49929:intron 25.0%
ACAAAATTTGGTCGCTAAAT+TGG - chr6.4:59136418-59136437 MS.gene49929:intron 30.0%
AGTACACCTCAAATTGTAAA+AGG + chr6.4:59135693-59135712 None:intergenic 30.0%
CACAAGCATTAATATCTTGT+TGG + chr6.4:59136495-59136514 None:intergenic 30.0%
GACAAGGAAATACAAAGAAA+AGG + chr6.4:59136175-59136194 None:intergenic 30.0%
GATTATGTGATATCATGACA+AGG + chr6.4:59136191-59136210 None:intergenic 30.0%
GCTCTAACTAAAATTTGACT+CGG - chr6.4:59135522-59135541 MS.gene49929:intron 30.0%
GTTCAATGGGAAGAATATAA+TGG + chr6.4:59135783-59135802 None:intergenic 30.0%
TCAGTCGAGTTAATTAAAGT+CGG - chr6.4:59134685-59134704 MS.gene49929:CDS 30.0%
! ACAATTTGAGGTGTACTTAA+GGG - chr6.4:59135696-59135715 MS.gene49929:intron 30.0%
! ACTTCACATGTTTAGTTCAA+TGG + chr6.4:59135797-59135816 None:intergenic 30.0%
! ATGCAAGAAGCACATATTAA+TGG - chr6.4:59136759-59136778 MS.gene49929:intron 30.0%
! CTTCACATGTTTAGTTCAAT+GGG + chr6.4:59135796-59135815 None:intergenic 30.0%
! GAGAATCTGAATTTGACTTA+AGG - chr6.4:59135217-59135236 MS.gene49929:intron 30.0%
! TACAATTTGAGGTGTACTTA+AGG - chr6.4:59135695-59135714 MS.gene49929:intron 30.0%
!! ACATATTAATGGATTAGCAG+TGG - chr6.4:59136770-59136789 MS.gene49929:intron 30.0%
!! TAGCGACCAAATTTTGTTAT+CGG + chr6.4:59136415-59136434 None:intergenic 30.0%
!! TAGGAGTATTGTAATGACAA+TGG - chr6.4:59135128-59135147 MS.gene49929:intron 30.0%
!!! ATTTCTATTTTTCAAGCCAG+TGG - chr6.4:59136527-59136546 MS.gene49929:intron 30.0%
ACACATGCAGAAAGATAGAT+AGG + chr6.4:59136148-59136167 None:intergenic 35.0%
ACCATAGAAAAAACAACACC+CGG - chr6.4:59135192-59135211 MS.gene49929:intron 35.0%
AGAAGAAGCAATATAGAGAG+TGG + chr6.4:59134544-59134563 None:intergenic 35.0%
ATCAATATGACGCGACAAAT+TGG + chr6.4:59135373-59135392 None:intergenic 35.0%
ATTGTTATACACATGGAGGA+TGG + chr6.4:59135927-59135946 None:intergenic 35.0%
CAACATTGTTATACACATGG+AGG + chr6.4:59135931-59135950 None:intergenic 35.0%
CACAACTATTATAGCAGACA+AGG + chr6.4:59135875-59135894 None:intergenic 35.0%
CTTGTCTGCTATAATAGTTG+TGG - chr6.4:59135873-59135892 MS.gene49929:intron 35.0%
GTGGATGTGTTATAATTACC+AGG + chr6.4:59134525-59134544 None:intergenic 35.0%
GTTTCAAACAAGGCAAAGAT+AGG - chr6.4:59135109-59135128 MS.gene49929:intron 35.0%
TGTGTTATAATTACCAGGAG+TGG + chr6.4:59134520-59134539 None:intergenic 35.0%
TTAGGTGAAATTAGAGCAAG+TGG + chr6.4:59134480-59134499 None:intergenic 35.0%
TTGTGTAGCATTACTCTGTA+TGG + chr6.4:59136654-59136673 None:intergenic 35.0%
! CTGCATCCTTTTACAATTTG+AGG - chr6.4:59135684-59135703 MS.gene49929:intron 35.0%
!! TTTGACTCGGAGAATCTTTA+AGG - chr6.4:59135535-59135554 MS.gene49929:intron 35.0%
!!! AGGTTTTGAACTATGATGCA+TGG + chr6.4:59136577-59136596 None:intergenic 35.0%
!!! GGTGTTGTTTTTTCTATGGT+TGG + chr6.4:59135192-59135211 None:intergenic 35.0%
!!! GTTGTTTTTTCTATGGTTGG+AGG + chr6.4:59135189-59135208 None:intergenic 35.0%
ATGCTCGTTAGGTTCTTAGA+CGG - chr6.4:59135588-59135607 MS.gene49929:intron 40.0%
GATGCATTGAGTAGTTGGTT+AGG + chr6.4:59134498-59134517 None:intergenic 40.0%
GCAATATCAACTGTCATTCC+AGG + chr6.4:59136729-59136748 None:intergenic 40.0%
GCAATGTAATGCGTTCATGT+TGG + chr6.4:59135504-59135523 None:intergenic 40.0%
GCTATAATAGTTGTGGTCAC+TGG - chr6.4:59135880-59135899 MS.gene49929:intron 40.0%
GGATAGAAAGAATGCTCGTT+AGG - chr6.4:59135577-59135596 MS.gene49929:intron 40.0%
GTCAAATTCAGATTCTCGAC+CGG + chr6.4:59135214-59135233 None:intergenic 40.0%
TCAAATTCAGATTCTCGACC+GGG + chr6.4:59135213-59135232 None:intergenic 40.0%
TGCATGCAATTGAGAGGAAA+TGG - chr6.4:59136828-59136847 MS.gene49929:CDS 40.0%
TGTAGCATTACTCTGTATGG+TGG + chr6.4:59136651-59136670 None:intergenic 40.0%
TTCCTCTCAATTGCATGCAA+TGG + chr6.4:59136827-59136846 None:intergenic 40.0%
! GTGTTGATGAAAGTGATACC+TGG - chr6.4:59136708-59136727 MS.gene49929:intron 40.0%
!! CGGAGAATCTTTAAGGTCTT+GGG - chr6.4:59135542-59135561 MS.gene49929:intron 40.0%
!! TCGGAGAATCTTTAAGGTCT+TGG - chr6.4:59135541-59135560 MS.gene49929:intron 40.0%
!!! ACCGGGTGTTGTTTTTTCTA+TGG + chr6.4:59135196-59135215 None:intergenic 40.0%
ACAATGGTAGCCAGTGGAAA+AGG - chr6.4:59135144-59135163 MS.gene49929:intron 45.0%
ACGAACATCATATACGCCAC+TGG + chr6.4:59136546-59136565 None:intergenic 45.0%
AGTTCAATTGAGCCAGCTGA+TGG - chr6.4:59136858-59136877 MS.gene49929:CDS 45.0%
GACCATTGCATGCAATTGAG+AGG - chr6.4:59136822-59136841 MS.gene49929:CDS 45.0%
GAGTGGATGCATTGAGTAGT+TGG + chr6.4:59134503-59134522 None:intergenic 45.0%
GTAATGACAATGGTAGCCAG+TGG - chr6.4:59135138-59135157 MS.gene49929:intron 45.0%
! TGGAAAAGGTGGTGGTTTGT+TGG - chr6.4:59135158-59135177 MS.gene49929:intron 45.0%
ATGGTAGCCAGTGGAAAAGG+TGG - chr6.4:59135147-59135166 MS.gene49929:intron 50.0%
CTACTCAATGCATCCACTCC+TGG - chr6.4:59134504-59134523 MS.gene49929:CDS 50.0%
TCAATTGAGCCAGCTGATGG+TGG - chr6.4:59136861-59136880 MS.gene49929:CDS 50.0%
! CAAACCACCACCTTTTCCAC+TGG + chr6.4:59135157-59135176 None:intergenic 50.0%
GGTCTTGGGTCTGAGTCTTG+TGG - chr6.4:59135556-59135575 MS.gene49929:intron 55.0%
GTAGCCAGTGGAAAAGGTGG+TGG - chr6.4:59135150-59135169 MS.gene49929:intron 55.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 59134450 59136890 59134450 ID=MS.gene49929
chr6.4 mRNA 59134450 59136890 59134450 ID=MS.gene49929.t1;Parent=MS.gene49929
chr6.4 exon 59136815 59136890 59136815 ID=MS.gene49929.t1.exon1;Parent=MS.gene49929.t1
chr6.4 CDS 59136815 59136890 59136815 ID=cds.MS.gene49929.t1;Parent=MS.gene49929.t1
chr6.4 exon 59136102 59136246 59136102 ID=MS.gene49929.t1.exon2;Parent=MS.gene49929.t1
chr6.4 CDS 59136102 59136246 59136102 ID=cds.MS.gene49929.t1;Parent=MS.gene49929.t1
chr6.4 exon 59134450 59134711 59134450 ID=MS.gene49929.t1.exon3;Parent=MS.gene49929.t1
chr6.4 CDS 59134450 59134711 59134450 ID=cds.MS.gene49929.t1;Parent=MS.gene49929.t1
Gene Sequence

>MS.gene49929

ATGAAAGCTTCATTACGCAGTGCTACTCCACTTGCTCTAATTTCACCTAACCAACTACTCAATGCATCCACTCCTGGTAATTATAACACATCCACTCTCTATATTGCTTCTTCTCACTTTCTTAAATTCAAAACTACTTTGCTTTGTTGATTAATTTCACAAACATCACAAGTTTTATATTTTATTTAATAATTATCTAGTTGTTAGAGGAAAAGTCCCTTGTATAATTTGAAGTTCAGTCGAGTTAATTAAAGTCGGACTAAAGATTGTTTCAGTATATAAACTCGGGTTATTTCAAACACTTTGACCATAAATTAAGCTCGTTAATCAATTGAGTCCAACTCAATTGAAGAAAATATTGATGAAATTGTGTAAGAAGGATATAACTAGTTGTTTTATATTTATGATATTTTTCCTAAAATTATAATAATAAAGGGATATAGAACGTATGCTCGTTAAGATATTTGAGCTCTAATTTTAATAGATTAAATATCAAAGATAGGAGTATTATAATGACTATGGTAGCCAGTGAAAATGTATTTCAGTTAAGACATTTCAGCTCTAATTTTAATAGACATTTCTCGTTAAAAAAATTGTATTAAGGTTCAAATGTTAATTGATACATATAAAATTTGTAATTACAGGTGATAAATATTTTGGTTTCAAACAAGGCAAAGATAGGAGTATTGTAATGACAATGGTAGCCAGTGGAAAAGGTGGTGGTTTGTTGGACAAGCCTCCAACCATAGAAAAAACAACACCCGGTCGAGAATCTGAATTTGACTTAAGGTAATACGAAAAAAGTTATCTTATTTGAACATCAAAATACAGAATGTGATACTGTATATATTTATCTTTATTTTTTTTTCTTACTTTTTATCTTATGTAACTTACACTCATATCAAAAGTATGCATAAATACCAATTTGTCGCGTCATATTGATACCATATTTCACATCAAAATTTTGATGTCAAAGTACCATTACTCTAACGATAACATTAGAAGTTGAAAGATTAGGGTAGTAAACATTACTTTAAATCGTCTCAAATTACCAACATGAACGCATTACATTGCTCTAACTAAAATTTGACTCGGAGAATCTTTAAGGTCTTGGGTCTGAGTCTTGTGGATAGAAAGAATGCTCGTTAGGTTCTTAGACGGACATTAATCATCGACAAGCTAATAGATACTTCACACCTAAAAAAACAGCTTCAAATTTTAAATTCAAATGTTGCTGCATCCTTTTACAATTTGAGGTGTACTTAAGGGACACTAGTAAATTATTTTATTAGGGACTTATATTGATAACATGAGCTATATAGTTCACATTCCATTATATTCTTCCCATTGAACTAAACATGTGAAGTTATTAGAACTGATTAAAGTAGATTGAAAAACATGTTGATCTTTATATTCAAGTTTCCTTGTCTGCTATAATAGTTGTGGTCACTGGTCATTTCAATTGCTAATGATTCCATCCTCCATGTGTATAACAATGTTGAAAATCAGTCTTTTTGCTTTCACAATTACTAACATTTAATAATAGGTCCACAATAATATTTTCATTTTTTTAGATAAACTAAGACATAATTAGTTGTCAAAAGTGGATATATGAATGAATGATTTCTTTTACTTCTTGTCTTTGAAAAGAAGTGTAACTATGAAGTTGAGAAAATCATTTTGTGGAAGCTCATTCCTATCTATCTTTCTGCATGTGTAGTCCCTTTTCTTTGTATTTCCTTGTCATGATATCACATAATCACATTAATTGAAAGGCAAAATGAGTAGTCAAAACTGAAAACCTATGTAACTACATAAACAATGTCATGTCATATATAGCGAGCGATTTAGAGATCGAAAATATCAAATTGGTCGTTAAATCGAGATCATTAACATTAGAAATTAGTGGTTTAACGACATTTCGTAAATCGATCTCTAAATTAATCAAAACTCATTTAGTAACCGATAACAAAATTTGGTCGCTAAATTGGTCGACTAATAGCGATTTTTTAGTACTTCAGATTGAAGACATGTTTCAGTGTCCAACAAGATATTAATGCTTGTGATTATATACATCATTTCTATTTTTCAAGCCAGTGGCGTATATGATGTTCGTGTCTGTATCCATGCATCATAGTTCAAAACCTTTGATGACATGTTTTATTGTTTTTGTTACTAGGAAATCAAAAAAGACATCTCCACCATACAGAGTAATGCTACACAATGACAACTATAACAAAAGAGAATATGTTGTGCAAGTGTTGATGAAAGTGATACCTGGAATGACAGTTGATATTGCAGTTAACGTCATGCAAGAAGCACATATTAATGGATTAGCAGTGGTCATTATTTGTGCTCAAGTTGATGCTGAAGACCATTGCATGCAATTGAGAGGAAATGGTCTTTTAAGTTCAATTGAGCCAGCTGATGGTGGTTGTTGA

Protein sequence

>MS.gene49929.t1

MKASLRSATPLALISPNQLLNASTPGDKYFGFKQGKDRSIVMTMVASGKGGGLLDKPPTIEKTTPGRESEFDLRKSKKTSPPYRVMLHNDNYNKREYVVQVLMKVIPGMTVDIAVNVMQEAHINGLAVVIICAQVDAEDHCMQLRGNGLLSSIEPADGGC