AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065502

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065502.t1 MTR_5g092680 97.358 265 7 0 1 265 1 265 0.0 536
MS.gene065502.t1 MTR_5g092680 96.981 265 7 1 1 265 1 264 0.0 530
MS.gene065502.t1 MTR_5g092680 98.020 202 4 0 64 265 1 202 6.59e-151 419
MS.gene065502.t1 MTR_3g051460 80.198 202 40 0 64 265 1 202 8.90e-126 356
MS.gene065502.t1 MTR_3g051460 69.874 239 61 2 30 265 7 237 9.31e-124 352
MS.gene065502.t1 MTR_3g051460 81.622 185 34 0 81 265 1 185 2.49e-117 333
MS.gene065502.t1 MTR_3g075380 63.452 197 63 2 71 265 7 196 7.39e-83 247
MS.gene065502.t1 MTR_8g076060 43.262 141 75 2 99 238 51 187 2.23e-27 104
MS.gene065502.t1 MTR_8g076060 42.000 150 82 2 99 247 51 196 3.78e-27 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065502.t1 AT4G25130 63.454 249 87 2 21 265 10 258 1.59e-117 337
MS.gene065502.t1 AT5G61640 73.399 203 54 0 63 265 27 229 4.39e-117 335
MS.gene065502.t1 AT5G61640 73.267 202 54 0 64 265 1 202 2.07e-116 332
MS.gene065502.t1 AT5G07470 74.752 202 51 0 64 265 1 202 1.19e-114 328
MS.gene065502.t1 AT5G07460 72.678 183 50 0 83 265 36 218 6.62e-102 296
MS.gene065502.t1 AT2G18030 34.826 201 119 4 42 241 3 192 6.69e-27 103
MS.gene065502.t1 AT2G18030 34.500 200 119 4 42 240 3 191 3.07e-26 103

Find 88 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTCAGCAGTTTGCTGCTTT+TGG 0.133192 5.2:-78228773 MS.gene065502:CDS
ATTCTCAAAGGCACATTGTT+TGG 0.290349 5.2:+78228589 None:intergenic
GAAAATAGCTAAAGAGTCTT+TGG 0.300627 5.2:-78227709 MS.gene065502:CDS
CACATTGTTTGGGATCATAT+TGG 0.309800 5.2:+78228600 None:intergenic
CTCAGCCCTGTAGAACTTCT+TGG 0.322376 5.2:+78227635 None:intergenic
CTTCTGAACAAACTTGGATT+TGG 0.322720 5.2:-78228866 MS.gene065502:CDS
AGGGCTTGGTTACAGTAAAA+TGG 0.338171 5.2:+78228897 None:intergenic
TGAGGGTAGGAACTTGGTTT+TGG 0.356279 5.2:+78228970 None:intergenic
AAGTTTGTTCAGAAGGTTCA+TGG 0.357858 5.2:+78228874 None:intergenic
ACTTATGAAGATGTGTGTTC+AGG 0.363577 5.2:-78228653 MS.gene065502:CDS
TACAACCTCATTGTGGTTTG+TGG 0.365802 5.2:+78228628 None:intergenic
TTCTCAAAGGCACATTGTTT+GGG 0.365969 5.2:+78228590 None:intergenic
TCGGTATATACCTGTCGATT+TGG 0.374244 5.2:+78228527 None:intergenic
GGAGCTGGACGTGGTGCTAT+AGG 0.378569 5.2:+78229030 None:intergenic
AGAATCTGCTTGATACGTTC+TGG 0.387186 5.2:-78228571 MS.gene065502:CDS
AATTAACCATAGCACCGAAT+TGG 0.392915 5.2:+78227537 None:intergenic
CTTTCTCAAGGTACTGTTGA+TGG 0.393321 5.2:+78227607 None:intergenic
TTTGGGGCTGGCTGCTTCTG+GGG 0.398460 5.2:-78228755 MS.gene065502:CDS
CAGAAGGTTCATGGAGGGCT+TGG 0.402252 5.2:+78228883 None:intergenic
GGAACAACAAGAGAAGCAAT+TGG 0.418797 5.2:-78227688 MS.gene065502:CDS
CAAACCACAATGAGGTTGTA+AGG 0.423126 5.2:-78228625 MS.gene065502:CDS
TTAGTCTTCCAGAGGGTGCC+TGG 0.428460 5.2:-78228725 MS.gene065502:CDS
CTTGAGAAAGGTGGTCGATT+CGG 0.429559 5.2:-78227594 MS.gene065502:CDS
TTCCTGCCAAGAAGTTCTAC+AGG 0.437198 5.2:-78227641 MS.gene065502:CDS
TACTAACTCCAGGCACCCTC+TGG 0.440966 5.2:+78228717 None:intergenic
ATGAACCTTCTGAACAAACT+TGG 0.442513 5.2:-78228872 MS.gene065502:CDS
CTTATGAAGATGTGTGTTCA+GGG 0.443891 5.2:-78228652 MS.gene065502:CDS
CAAATCCAAGTTTGTTCAGA+AGG 0.446426 5.2:+78228867 None:intergenic
GGGCTTGGTTACAGTAAAAT+GGG 0.455190 5.2:+78228898 None:intergenic
CGTGGTGCTATAGGCGCTGC+TGG 0.457191 5.2:+78229039 None:intergenic
ACCTGTCGATTTGGCGTAGT+AGG 0.463222 5.2:+78228536 None:intergenic
AAGATGAGAATTTGTGGAGC+AGG 0.472195 5.2:-78230618 MS.gene065502:intron
TAGGATTGTGCAAAAGCCCC+TGG 0.477867 5.2:+78228675 None:intergenic
AGAAAGTGAGGGTAGGAACT+TGG 0.478713 5.2:+78228964 None:intergenic
GAACAACAAGAGAAGCAATT+GGG 0.490289 5.2:-78227687 MS.gene065502:CDS
CATGGATTCAACCATTCCTC+AGG 0.492517 5.2:-78228822 MS.gene065502:CDS
GTGGGAACACAGTACAGATC+TGG 0.492891 5.2:-78227759 MS.gene065502:CDS
GGTGCTGGTATATCGTCATC+TGG 0.500288 5.2:+78228797 None:intergenic
AAGGATGCAATGATCCAATT+CGG 0.509233 5.2:-78227551 MS.gene065502:CDS
TCCTACTACGCCAAATCGAC+AGG 0.516001 5.2:-78228537 MS.gene065502:intron
TTCAAGCAATCTGCTGCTAA+AGG 0.517342 5.2:-78227570 MS.gene065502:CDS
TTGTTGAAGATGAGAATTTG+TGG 0.519052 5.2:-78230624 None:intergenic
TGCAAGGTCATCGGAGAGCA+TGG 0.519772 5.2:-78228840 MS.gene065502:CDS
ACAGTAAAATGGGGACGGGT+TGG 0.522086 5.2:+78228908 None:intergenic
AAAGCAGCAAACTGCTGACC+TGG 0.522845 5.2:+78228776 None:intergenic
AACAAACACTAGCAAGGAGC+TGG 0.528081 5.2:+78229015 None:intergenic
GAACACACATCTTCATAAGT+AGG 0.536289 5.2:+78228656 None:intergenic
AACTTGGATTTGGCAGTGCA+AGG 0.536639 5.2:-78228856 MS.gene065502:CDS
AATGATCCAATTCGGTGCTA+TGG 0.541844 5.2:-78227543 MS.gene065502:CDS
GGTTACAGTAAAATGGGGAC+GGG 0.546721 5.2:+78228904 None:intergenic
GACAGAAAATCTAGAAAGTG+AGG 0.547056 5.2:+78228952 None:intergenic
ACCTCTGTCTTACTAACTCC+AGG 0.548827 5.2:+78228707 None:intergenic
AACAAGAGAAGCAATTGGGC+AGG 0.550479 5.2:-78227683 MS.gene065502:CDS
GCAAACTGCTGACCTGGTGC+TGG 0.552206 5.2:+78228782 None:intergenic
GTCATCTGGTCCCTGAGGAA+TGG 0.556094 5.2:+78228811 None:intergenic
GCCCTGTAGAACTTCTTGGC+AGG 0.556183 5.2:+78227639 None:intergenic
GGAGTTAGTAAGACAGAGGT+TGG 0.557076 5.2:-78228704 MS.gene065502:CDS
CACTAGCAAGGAGCTGGACG+TGG 0.557263 5.2:+78229021 None:intergenic
ATTTGGCAGTGCAAGGTCAT+CGG 0.557290 5.2:-78228849 MS.gene065502:CDS
ACAGAGGTTGGTTACACCCA+GGG 0.566736 5.2:-78228692 MS.gene065502:CDS
CGTATCAAGCAGATTCTCAA+AGG 0.580362 5.2:+78228577 None:intergenic
CATCAACAGTACCTTGAGAA+AGG 0.580843 5.2:-78227606 MS.gene065502:CDS
TGGTTACAGTAAAATGGGGA+CGG 0.587074 5.2:+78228903 None:intergenic
CAAGAAGTTCTACAGGGCTG+AGG 0.588571 5.2:-78227634 MS.gene065502:CDS
GTGTTGAATTAGTCTTCCAG+AGG 0.595505 5.2:-78228733 MS.gene065502:CDS
TTGTTCAGAAGGTTCATGGA+GGG 0.602277 5.2:+78228878 None:intergenic
CGAATCGACCACCTTTCTCA+AGG 0.605526 5.2:+78227595 None:intergenic
AGGATTGTGCAAAAGCCCCT+GGG 0.609672 5.2:+78228676 None:intergenic
AAACCACAATGAGGTTGTAA+GGG 0.613260 5.2:-78228624 MS.gene065502:CDS
AGAAGAAACAAACACTAGCA+AGG 0.615666 5.2:+78229009 None:intergenic
ACAGAAAATCTAGAAAGTGA+GGG 0.620493 5.2:+78228953 None:intergenic
GACAGAGGTTGGTTACACCC+AGG 0.622309 5.2:-78228693 MS.gene065502:CDS
AAAATCTAGAAAGTGAGGGT+AGG 0.627880 5.2:+78228957 None:intergenic
GGACCCTTACAACCTCATTG+TGG 0.628278 5.2:+78228621 None:intergenic
TGTTGAATTAGTCTTCCAGA+GGG 0.628480 5.2:-78228732 MS.gene065502:CDS
TCCTGCCAAGAAGTTCTACA+GGG 0.646626 5.2:-78227640 MS.gene065502:CDS
ATGGATTCAACCATTCCTCA+GGG 0.661571 5.2:-78228821 MS.gene065502:CDS
CAACAGTACCTTGAGAAAGG+TGG 0.667397 5.2:-78227603 MS.gene065502:CDS
CGGGTTGGTGAAAACAAGCA+AGG 0.672554 5.2:+78228923 None:intergenic
CAGAGGTTGGTTACACCCAG+GGG 0.676119 5.2:-78228691 MS.gene065502:CDS
GATGACGATATACCAGCACC+AGG 0.678617 5.2:-78228794 MS.gene065502:CDS
GCCTGGAGTTAGTAAGACAG+AGG 0.689093 5.2:-78228708 MS.gene065502:CDS
TATGTGACAGGGGAATGATG+TGG 0.690238 5.2:-78227778 MS.gene065502:intron
ATGTGACAGGGGAATGATGT+GGG 0.696486 5.2:-78227777 MS.gene065502:intron
ATATCGTCATCTGGTCCCTG+AGG 0.697908 5.2:+78228806 None:intergenic
TTTGTTCAGAAGGTTCATGG+AGG 0.703199 5.2:+78228877 None:intergenic
GGCTTGGTTACAGTAAAATG+GGG 0.703897 5.2:+78228899 None:intergenic
AGGGACCACAAACCACAATG+AGG 0.713909 5.2:-78228633 MS.gene065502:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAAAAAGGAA+TGG + chr5.2:78227850-78227869 None:intergenic 10.0%
!! AAATACTGTTAAAAAACATA+AGG + chr5.2:78229003-78229022 None:intergenic 15.0%
!! AAATATAGTGAAAATTGAAA+AGG + chr5.2:78227653-78227672 None:intergenic 15.0%
!! AAATTAGTGTTTAATGATAA+TGG - chr5.2:78228562-78228581 MS.gene065502:CDS 15.0%
!! AATACTGTTAAAAAACATAA+GGG + chr5.2:78229002-78229021 None:intergenic 15.0%
!! TGTGAATTTAATAACAAATA+AGG + chr5.2:78227950-78227969 None:intergenic 15.0%
!!! ATGATTATAATTTCTAGATA+AGG - chr5.2:78229061-78229080 MS.gene065502:intron 15.0%
!!! TATATTGATAATAATAACAG+AGG - chr5.2:78228423-78228442 MS.gene065502:intron 15.0%
!!! TGATTATAATTTCTAGATAA+GGG - chr5.2:78229062-78229081 MS.gene065502:intron 15.0%
!!! TTTTAACAGTATTTTGAAAA+TGG - chr5.2:78229009-78229028 MS.gene065502:CDS 15.0%
!! AAAAAAAAAAAAAGGAATGG+AGG + chr5.2:78227847-78227866 None:intergenic 20.0%
!! AAAAAAAAAAAAGGAATGGA+GGG + chr5.2:78227846-78227865 None:intergenic 20.0%
!! AGAATGCATTTATACATAAA+AGG + chr5.2:78228240-78228259 None:intergenic 20.0%
!! ATGCATTAGAAATTTATAGA+TGG - chr5.2:78228331-78228350 MS.gene065502:intron 20.0%
!! CACTAAAAAAACTAAAAACA+AGG + chr5.2:78227627-78227646 None:intergenic 20.0%
!! GAATGCATTTATACATAAAA+GGG + chr5.2:78228239-78228258 None:intergenic 20.0%
!! TATAAGCTAGACAATATAAA+TGG + chr5.2:78227784-78227803 None:intergenic 20.0%
!! TCAAAAATGAACATTTAAGA+TGG + chr5.2:78228921-78228940 None:intergenic 20.0%
!! TGAATTGTAGAAACATAAAA+GGG + chr5.2:78227593-78227612 None:intergenic 20.0%
!! TTATAAACAAACGAACAATT+CGG + chr5.2:78229649-78229668 None:intergenic 20.0%
!!! AACTCTTTTACTTTGATAAT+AGG - chr5.2:78228894-78228913 MS.gene065502:CDS 20.0%
!!! AAGATTTAGGACTAATTTAA+GGG + chr5.2:78227820-78227839 None:intergenic 20.0%
!!! AATTTACTAAAAGTACATGT+TGG + chr5.2:78229991-78230010 None:intergenic 20.0%
!!! ACAATTGATAGTATTGATAA+AGG - chr5.2:78228283-78228302 MS.gene065502:intron 20.0%
!!! ACATGTACTTTTAGTAAATT+TGG - chr5.2:78229991-78230010 MS.gene065502:intron 20.0%
!!! AGTATAAAACCATTTTGATA+TGG - chr5.2:78230103-78230122 MS.gene065502:intron 20.0%
!!! CAATTGATAGTATTGATAAA+GGG - chr5.2:78228284-78228303 MS.gene065502:intron 20.0%
!!! CATGTACTTTTAGTAAATTT+GGG - chr5.2:78229992-78230011 MS.gene065502:intron 20.0%
!!! TAAGATTTAGGACTAATTTA+AGG + chr5.2:78227821-78227840 None:intergenic 20.0%
!!! TATTTTTCATTCAGTTTTCT+TGG - chr5.2:78228960-78228979 MS.gene065502:CDS 20.0%
!!! TGTAAATGGTCTTATAATTA+GGG - chr5.2:78228381-78228400 MS.gene065502:intron 20.0%
!!! TTGTAAATGGTCTTATAATT+AGG - chr5.2:78228380-78228399 MS.gene065502:intron 20.0%
!!! TTTTTTTATGTTTTAGCTCT+TGG - chr5.2:78227865-78227884 MS.gene065502:intron 20.0%
! AAACATCAAAAGAAAAGGAA+TGG - chr5.2:78228094-78228113 MS.gene065502:intron 25.0%
! ACAACTGCTTTAATAGTTTA+TGG - chr5.2:78227988-78228007 MS.gene065502:intron 25.0%
! ATCAAAATGATTAACATTGG+AGG + chr5.2:78229849-78229868 None:intergenic 25.0%
! ATTTCCAATATGAGAATCAA+AGG - chr5.2:78228626-78228645 MS.gene065502:CDS 25.0%
! ATTTCCTTTGATTCTCATAT+TGG + chr5.2:78228633-78228652 None:intergenic 25.0%
! CACTAAAACATCAAAAGTTA+TGG - chr5.2:78228053-78228072 MS.gene065502:intron 25.0%
! CTATACATGTATAGAAGAAA+TGG - chr5.2:78228489-78228508 MS.gene065502:intron 25.0%
! CTGAATTGTAGAAACATAAA+AGG + chr5.2:78227594-78227613 None:intergenic 25.0%
! GACCATGAATATCATTAATT+AGG - chr5.2:78227683-78227702 MS.gene065502:CDS 25.0%
! GAGTAGTTTACAAAAATTAC+AGG - chr5.2:78228155-78228174 MS.gene065502:intron 25.0%
! GCTATCAAAATGATTAACAT+TGG + chr5.2:78229852-78229871 None:intergenic 25.0%
! GTAAACTACTCTCATATTAA+GGG + chr5.2:78228146-78228165 None:intergenic 25.0%
! TACAGTATCAACAAACAAAA+GGG + chr5.2:78228873-78228892 None:intergenic 25.0%
! TAGATTACTCCATATCAAAA+TGG + chr5.2:78230115-78230134 None:intergenic 25.0%
! TATAGATGGTCTTCTATTTA+AGG - chr5.2:78228345-78228364 MS.gene065502:intron 25.0%
! TATGAGAATCAAAGGAAATA+AGG - chr5.2:78228634-78228653 MS.gene065502:CDS 25.0%
! TGTAAACTACTCTCATATTA+AGG + chr5.2:78228147-78228166 None:intergenic 25.0%
! TTACAGTATCAACAAACAAA+AGG + chr5.2:78228874-78228893 None:intergenic 25.0%
! TTGAACAGAAAAGAAACAAA+GGG + chr5.2:78230165-78230184 None:intergenic 25.0%
!! ATTTTCATACCTAAACATCT+AGG - chr5.2:78228722-78228741 MS.gene065502:CDS 25.0%
!!! CTCTAAAACATCAAAAGAAA+AGG - chr5.2:78228089-78228108 MS.gene065502:intron 25.0%
!!! GACAAACACTTTTTTGTAAA+TGG - chr5.2:78228367-78228386 MS.gene065502:intron 25.0%
ACAAGACAAAATGTTAGGTA+AGG + chr5.2:78230142-78230161 None:intergenic 30.0%
ACAGAAAATCTAGAAAGTGA+GGG + chr5.2:78229204-78229223 None:intergenic 30.0%
ATCAAAAGAAAAGGAATGGA+GGG - chr5.2:78228098-78228117 MS.gene065502:intron 30.0%
ATTCACATGTGATAAACCAA+AGG - chr5.2:78227963-78227982 MS.gene065502:intron 30.0%
CTTGAACAGAAAAGAAACAA+AGG + chr5.2:78230166-78230185 None:intergenic 30.0%
GAAAATAGCTAAAGAGTCTT+TGG - chr5.2:78230445-78230464 MS.gene065502:intron 30.0%
GAATAATAAGTCTAGACTTG+TGG - chr5.2:78229754-78229773 MS.gene065502:intron 30.0%
GGCCTAATTAATGATATTCA+TGG + chr5.2:78227688-78227707 None:intergenic 30.0%
TATTGTATCAATATGACAGC+AGG + chr5.2:78227709-78227728 None:intergenic 30.0%
! GTATTGATAAAGGGTATAGT+TGG - chr5.2:78228293-78228312 MS.gene065502:intron 30.0%
! TCTCATATTAAGGGAGTATA+TGG + chr5.2:78228137-78228156 None:intergenic 30.0%
! TTTATGGTGTTTTCCAACAA+GGG - chr5.2:78228004-78228023 MS.gene065502:intron 30.0%
!! AATGGCTCTTATGTTTTTGA+AGG - chr5.2:78229027-78229046 MS.gene065502:CDS 30.0%
!! AATGGTCTTATAATTAGGGA+AGG - chr5.2:78228385-78228404 MS.gene065502:intron 30.0%
!! ACAAGACAATCATTTTTCCT+GGG + chr5.2:78230030-78230049 None:intergenic 30.0%
!! ATGGCTCTTATGTTTTTGAA+GGG - chr5.2:78229028-78229047 MS.gene065502:CDS 30.0%
AAAATCTAGAAAGTGAGGGT+AGG + chr5.2:78229200-78229219 None:intergenic 35.0%
AAACCACAATGAGGTTGTAA+GGG - chr5.2:78229530-78229549 MS.gene065502:intron 35.0%
AAGGATGCAATGATCCAATT+CGG - chr5.2:78230603-78230622 MS.gene065502:intron 35.0%
AAGGGACAAGACAAAATGTT+AGG + chr5.2:78230147-78230166 None:intergenic 35.0%
ACTAAGTAAGTACATGACAC+TGG + chr5.2:78228796-78228815 None:intergenic 35.0%
ACTCATAGTTCATGTTTAGG+AGG - chr5.2:78228599-78228618 MS.gene065502:CDS 35.0%
ACTTATGAAGATGTGTGTTC+AGG - chr5.2:78229501-78229520 MS.gene065502:intron 35.0%
AGAAGAAACAAACACTAGCA+AGG + chr5.2:78229148-78229167 None:intergenic 35.0%
AGCTTAGTAATTGTGACATC+TGG - chr5.2:78230346-78230365 MS.gene065502:intron 35.0%
AGGAATGAACATGCATGATT+AGG - chr5.2:78228654-78228673 MS.gene065502:CDS 35.0%
ATGAACCTTCTGAACAAACT+TGG - chr5.2:78229282-78229301 MS.gene065502:intron 35.0%
ATTAAAGCAGTTGTGACCTT+TGG + chr5.2:78227982-78228001 None:intergenic 35.0%
CATCAAAAGAAAAGGAATGG+AGG - chr5.2:78228097-78228116 MS.gene065502:intron 35.0%
CTCATAGTTCATGTTTAGGA+GGG - chr5.2:78228600-78228619 MS.gene065502:CDS 35.0%
CTTATGAAGATGTGTGTTCA+GGG - chr5.2:78229502-78229521 MS.gene065502:intron 35.0%
CTTCTGAACAAACTTGGATT+TGG - chr5.2:78229288-78229307 MS.gene065502:intron 35.0%
GAACAACAAGAGAAGCAATT+GGG - chr5.2:78230467-78230486 MS.gene065502:intron 35.0%
GAACACACATCTTCATAAGT+AGG + chr5.2:78229501-78229520 None:intergenic 35.0%
GAATGAACATGCATGATTAG+GGG - chr5.2:78228656-78228675 MS.gene065502:CDS 35.0%
GACAGAAAATCTAGAAAGTG+AGG + chr5.2:78229205-78229224 None:intergenic 35.0%
GCAACTCATAGTTCATGTTT+AGG - chr5.2:78228596-78228615 MS.gene065502:CDS 35.0%
GGAATGAACATGCATGATTA+GGG - chr5.2:78228655-78228674 MS.gene065502:CDS 35.0%
GTCTTATAATTAGGGAAGGA+GGG - chr5.2:78228389-78228408 MS.gene065502:intron 35.0%
TACTAAGCTCTTACCATTCA+TGG + chr5.2:78230335-78230354 None:intergenic 35.0%
TGATTTCCACTTTCCATGAA+TGG - chr5.2:78230319-78230338 MS.gene065502:intron 35.0%
TTATTCAGATCAGCAAAGTC+AGG + chr5.2:78229740-78229759 None:intergenic 35.0%
TTTCTCAAGTTTACCCTTGT+TGG + chr5.2:78228020-78228039 None:intergenic 35.0%
! AAGTTTGTTCAGAAGGTTCA+TGG + chr5.2:78229283-78229302 None:intergenic 35.0%
! ATTCTCAAAGGCACATTGTT+TGG + chr5.2:78229568-78229587 None:intergenic 35.0%
! CAAATCCAAGTTTGTTCAGA+AGG + chr5.2:78229290-78229309 None:intergenic 35.0%
! CACATTGTTTGGGATCATAT+TGG + chr5.2:78229557-78229576 None:intergenic 35.0%
! GTTTATGGTGTTTTCCAACA+AGG - chr5.2:78228003-78228022 MS.gene065502:intron 35.0%
! TTCTCAAAGGCACATTGTTT+GGG + chr5.2:78229567-78229586 None:intergenic 35.0%
! TTCTTTTCTGTTCAAGACTG+TGG - chr5.2:78230170-78230189 MS.gene065502:intron 35.0%
! TTGTGGATTGTCAACATTGA+TGG - chr5.2:78229771-78229790 MS.gene065502:intron 35.0%
!! GACAAGACAATCATTTTTCC+TGG + chr5.2:78230031-78230050 None:intergenic 35.0%
!! TGTTGAATTAGTCTTCCAGA+GGG - chr5.2:78229422-78229441 MS.gene065502:intron 35.0%
!! TTAGCTATTTTCTCTTGCTC+AGG + chr5.2:78230437-78230456 None:intergenic 35.0%
!!! ATGAGCCTCTGTTTTTTGAA+CGG + chr5.2:78228451-78228470 None:intergenic 35.0%
!!! GAGTTCTTTTTTGTCTCAAG+TGG - chr5.2:78229709-78229728 MS.gene065502:intron 35.0%
AAAGAAGAAACGGCAAAACC+TGG + chr5.2:78228839-78228858 None:intergenic 40.0%
AGAATCTGCTTGATACGTTC+TGG - chr5.2:78229583-78229602 MS.gene065502:intron 40.0%
ATGGATTCAACCATTCCTCA+GGG - chr5.2:78229333-78229352 MS.gene065502:intron 40.0%
CAAACCACAATGAGGTTGTA+AGG - chr5.2:78229529-78229548 MS.gene065502:intron 40.0%
CAATTCCTGCCTAGATGTTT+AGG + chr5.2:78228734-78228753 None:intergenic 40.0%
CATCAACAGTACCTTGAGAA+AGG - chr5.2:78230548-78230567 MS.gene065502:intron 40.0%
CGTATCAAGCAGATTCTCAA+AGG + chr5.2:78229580-78229599 None:intergenic 40.0%
CTCTTACCATTCATGGAAAG+TGG + chr5.2:78230328-78230347 None:intergenic 40.0%
CTGGATCTTTGTATGTGACA+GGG - chr5.2:78230365-78230384 MS.gene065502:intron 40.0%
GAACTCAATTCGCAAACAAG+AGG + chr5.2:78229695-78229714 None:intergenic 40.0%
GAATGGAGGGAGTAAGATTT+AGG + chr5.2:78227833-78227852 None:intergenic 40.0%
GCTGCACTCAAAAGAAGAAA+CGG + chr5.2:78228849-78228868 None:intergenic 40.0%
GGAACAACAAGAGAAGCAAT+TGG - chr5.2:78230466-78230485 MS.gene065502:intron 40.0%
TACAACCTCATTGTGGTTTG+TGG + chr5.2:78229529-78229548 None:intergenic 40.0%
TCATACCTAAACATCTAGGC+AGG - chr5.2:78228726-78228745 MS.gene065502:CDS 40.0%
TCTGGATCTTTGTATGTGAC+AGG - chr5.2:78230364-78230383 MS.gene065502:intron 40.0%
TCTTGTCACTGAGTATGTGA+TGG - chr5.2:78230044-78230063 MS.gene065502:intron 40.0%
TGGATCTTTGTATGTGACAG+GGG - chr5.2:78230366-78230385 MS.gene065502:intron 40.0%
TGGTTACAGTAAAATGGGGA+CGG + chr5.2:78229254-78229273 None:intergenic 40.0%
TTCAAGCAATCTGCTGCTAA+AGG - chr5.2:78230584-78230603 MS.gene065502:intron 40.0%
TTGGGTCAAATCAAATTCCC+AGG - chr5.2:78230010-78230029 MS.gene065502:intron 40.0%
TTGTTCAGAAGGTTCATGGA+GGG + chr5.2:78229279-78229298 None:intergenic 40.0%
TTTGTTCAGAAGGTTCATGG+AGG + chr5.2:78229280-78229299 None:intergenic 40.0%
! AGCTCTTGGCACAAGTTTTA+AGG - chr5.2:78227879-78227898 MS.gene065502:intron 40.0%
! AGGGCTTGGTTACAGTAAAA+TGG + chr5.2:78229260-78229279 None:intergenic 40.0%
! GGCTTGGTTACAGTAAAATG+GGG + chr5.2:78229258-78229277 None:intergenic 40.0%
! GGGCTTGGTTACAGTAAAAT+GGG + chr5.2:78229259-78229278 None:intergenic 40.0%
! GGTCTTATAATTAGGGAAGG+AGG - chr5.2:78228388-78228407 MS.gene065502:intron 40.0%
! TCGGTATATACCTGTCGATT+TGG + chr5.2:78229630-78229649 None:intergenic 40.0%
!! AATGATCCAATTCGGTGCTA+TGG - chr5.2:78230611-78230630 MS.gene065502:intron 40.0%
!! CTTTCTCAAGGTACTGTTGA+TGG + chr5.2:78230550-78230569 None:intergenic 40.0%
!! GTGTTGAATTAGTCTTCCAG+AGG - chr5.2:78229421-78229440 MS.gene065502:intron 40.0%
AAACATAAGGGCCACCAAGT+TGG + chr5.2:78228990-78229009 None:intergenic 45.0%
AACAAACACTAGCAAGGAGC+TGG + chr5.2:78229142-78229161 None:intergenic 45.0%
AACAAGAGAAGCAATTGGGC+AGG - chr5.2:78230471-78230490 MS.gene065502:intron 45.0%
AACATAAGGGCCACCAAGTT+GGG + chr5.2:78228989-78229008 None:intergenic 45.0%
AACTTGGATTTGGCAGTGCA+AGG - chr5.2:78229298-78229317 MS.gene065502:intron 45.0%
ACCTCTGTCTTACTAACTCC+AGG + chr5.2:78229450-78229469 None:intergenic 45.0%
AGAAACGGCAAAACCTGGAA+AGG + chr5.2:78228834-78228853 None:intergenic 45.0%
AGAAAGTGAGGGTAGGAACT+TGG + chr5.2:78229193-78229212 None:intergenic 45.0%
AGGCTCCGTTCAAAAAACAG+AGG - chr5.2:78228443-78228462 MS.gene065502:intron 45.0%
ATGTGACAGGGGAATGATGT+GGG - chr5.2:78230377-78230396 MS.gene065502:intron 45.0%
ATTTGGCAGTGCAAGGTCAT+CGG - chr5.2:78229305-78229324 MS.gene065502:intron 45.0%
CAACAGTACCTTGAGAAAGG+TGG - chr5.2:78230551-78230570 MS.gene065502:intron 45.0%
CATGGATTCAACCATTCCTC+AGG - chr5.2:78229332-78229351 MS.gene065502:intron 45.0%
CTTGAGAAAGGTGGTCGATT+CGG - chr5.2:78230560-78230579 MS.gene065502:intron 45.0%
GAGAATTTGTGGAGCAGGTA+TGG - chr5.2:78227541-78227560 MS.gene065502:CDS 45.0%
GGAGTTAGTAAGACAGAGGT+TGG - chr5.2:78229450-78229469 MS.gene065502:intron 45.0%
GGTTACAGTAAAATGGGGAC+GGG + chr5.2:78229253-78229272 None:intergenic 45.0%
TATGTGACAGGGGAATGATG+TGG - chr5.2:78230376-78230395 MS.gene065502:intron 45.0%
TCCTGCCAAGAAGTTCTACA+GGG - chr5.2:78230514-78230533 MS.gene065502:intron 45.0%
TTCCTGCCAAGAAGTTCTAC+AGG - chr5.2:78230513-78230532 MS.gene065502:intron 45.0%
! GTTTTCTTGGCATCCCAACT+TGG - chr5.2:78228973-78228992 MS.gene065502:CDS 45.0%
! TGAGGGTAGGAACTTGGTTT+TGG + chr5.2:78229187-78229206 None:intergenic 45.0%
!!! GTCAGCAGTTTGCTGCTTTT+GGG - chr5.2:78229382-78229401 MS.gene065502:intron 45.0%
!!! TCAGCAGTTTGCTGCTTTTG+GGG - chr5.2:78229383-78229402 MS.gene065502:intron 45.0%
!! CATAAAAAAAAAAAAAAAAA+AGG + chr5.2:78227855-78227874 None:intergenic 5.0%
AAAGCAGCAAACTGCTGACC+TGG + chr5.2:78229381-78229400 None:intergenic 50.0%
ACAGTAAAATGGGGACGGGT+TGG + chr5.2:78229249-78229268 None:intergenic 50.0%
AGGATTGTGCAAAAGCCCCT+GGG + chr5.2:78229481-78229500 None:intergenic 50.0%
AGGGACCACAAACCACAATG+AGG - chr5.2:78229521-78229540 MS.gene065502:intron 50.0%
ATATCGTCATCTGGTCCCTG+AGG + chr5.2:78229351-78229370 None:intergenic 50.0%
CAAGAAGTTCTACAGGGCTG+AGG - chr5.2:78230520-78230539 MS.gene065502:intron 50.0%
CGAATCGACCACCTTTCTCA+AGG + chr5.2:78230562-78230581 None:intergenic 50.0%
CTCAGCCCTGTAGAACTTCT+TGG + chr5.2:78230522-78230541 None:intergenic 50.0%
GATGACGATATACCAGCACC+AGG - chr5.2:78229360-78229379 MS.gene065502:intron 50.0%
GCAAAACCTGGAAAGGACTC+GGG + chr5.2:78228827-78228846 None:intergenic 50.0%
GCCTGGAGTTAGTAAGACAG+AGG - chr5.2:78229446-78229465 MS.gene065502:intron 50.0%
GGACCCTTACAACCTCATTG+TGG + chr5.2:78229536-78229555 None:intergenic 50.0%
GGCAAAACCTGGAAAGGACT+CGG + chr5.2:78228828-78228847 None:intergenic 50.0%
GTGGGAACACAGTACAGATC+TGG - chr5.2:78230395-78230414 MS.gene065502:intron 50.0%
TAGGATTGTGCAAAAGCCCC+TGG + chr5.2:78229482-78229501 None:intergenic 50.0%
TCCTACTACGCCAAATCGAC+AGG - chr5.2:78229617-78229636 MS.gene065502:intron 50.0%
TTCTTGGCATCCCAACTTGG+TGG - chr5.2:78228976-78228995 MS.gene065502:CDS 50.0%
TTGATTCCCGAGTCCTTTCC+AGG - chr5.2:78228818-78228837 MS.gene065502:CDS 50.0%
! ACAGAGGTTGGTTACACCCA+GGG - chr5.2:78229462-78229481 MS.gene065502:intron 50.0%
!! ACCTGTCGATTTGGCGTAGT+AGG + chr5.2:78229621-78229640 None:intergenic 50.0%
!! CGGGTTGGTGAAAACAAGCA+AGG + chr5.2:78229234-78229253 None:intergenic 50.0%
!! GGTGCTGGTATATCGTCATC+TGG + chr5.2:78229360-78229379 None:intergenic 50.0%
!!! GAACTTGGTTTTGGCAGGAG+TGG + chr5.2:78229178-78229197 None:intergenic 50.0%
!!! GGTAGGAACTTGGTTTTGGC+AGG + chr5.2:78229183-78229202 None:intergenic 50.0%
!!! GGTCAGCAGTTTGCTGCTTT+TGG - chr5.2:78229381-78229400 MS.gene065502:intron 50.0%
CAGAAGGTTCATGGAGGGCT+TGG + chr5.2:78229274-78229293 None:intergenic 55.0%
GCCCTGTAGAACTTCTTGGC+AGG + chr5.2:78230518-78230537 None:intergenic 55.0%
GTCATCTGGTCCCTGAGGAA+TGG + chr5.2:78229346-78229365 None:intergenic 55.0%
TACTAACTCCAGGCACCCTC+TGG + chr5.2:78229440-78229459 None:intergenic 55.0%
TGCAAGGTCATCGGAGAGCA+TGG - chr5.2:78229314-78229333 MS.gene065502:intron 55.0%
! CAGAGGTTGGTTACACCCAG+GGG - chr5.2:78229463-78229482 MS.gene065502:intron 55.0%
! GACAGAGGTTGGTTACACCC+AGG - chr5.2:78229461-78229480 MS.gene065502:intron 55.0%
!! GGTTTTGGCAGGAGTGGAGA+AGG + chr5.2:78229172-78229191 None:intergenic 55.0%
!! TTAGTCTTCCAGAGGGTGCC+TGG - chr5.2:78229429-78229448 MS.gene065502:intron 55.0%
!!! CAGTTTGCTGCTTTTGGGGC+TGG - chr5.2:78229387-78229406 MS.gene065502:intron 55.0%
!!! TTTTGGGGCTGGCTGCTTCT+GGG - chr5.2:78229398-78229417 MS.gene065502:intron 55.0%
CACTAGCAAGGAGCTGGACG+TGG + chr5.2:78229136-78229155 None:intergenic 60.0%
GCAAACTGCTGACCTGGTGC+TGG + chr5.2:78229375-78229394 None:intergenic 60.0%
!! GGAGCTGGACGTGGTGCTAT+AGG + chr5.2:78229127-78229146 None:intergenic 60.0%
!! TTTGGGGCTGGCTGCTTCTG+GGG - chr5.2:78229399-78229418 MS.gene065502:intron 60.0%
!!! CTTTTGGGGCTGGCTGCTTC+TGG - chr5.2:78229397-78229416 MS.gene065502:intron 60.0%
!! CGTGGTGCTATAGGCGCTGC+TGG + chr5.2:78229118-78229137 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 78227539 78230637 78227539 ID=MS.gene065502
chr5.2 mRNA 78227539 78230637 78227539 ID=MS.gene065502.t1;Parent=MS.gene065502
chr5.2 exon 78230619 78230637 78230619 ID=MS.gene065502.t1.exon1;Parent=MS.gene065502.t1
chr5.2 CDS 78230619 78230637 78230619 ID=cds.MS.gene065502.t1;Parent=MS.gene065502.t1
chr5.2 exon 78228538 78229064 78228538 ID=MS.gene065502.t1.exon2;Parent=MS.gene065502.t1
chr5.2 CDS 78228538 78229064 78228538 ID=cds.MS.gene065502.t1;Parent=MS.gene065502.t1
chr5.2 exon 78227539 78227790 78227539 ID=MS.gene065502.t1.exon3;Parent=MS.gene065502.t1
chr5.2 CDS 78227539 78227790 78227539 ID=cds.MS.gene065502.t1;Parent=MS.gene065502.t1
Gene Sequence

>MS.gene065502

ATGAGAATTTGTGGAGCAGGTATGGTGTTCATATTTCATTTCTTTGTATTTCCCTTTTATGTTTCTACAATTCAGTATCATGTGACCTTGTTTTTAGTTTTTTTAGTGATTCCTTTTCAATTTTCACTATATTTAAATATCAATGACCATGAATATCATTAATTAGGCCTGCTGTCATATTGATACAATATTAAAATGTCAATTTTTTATTTATTTGTTTAATATTTGTTACAATTATGAATCCATTTATATTGTCTAGCTTATAATATGCATATGTTCCCTTAAATTAGTCCTAAATCTTACTCCCTCCATTCCTTTTTTTTTTTTTTTTTTATGTTTTAGCTCTTGGCACAAGTTTTAAGGAGTAATTAAAGTATATAAATATTTATGTGTTTTTGACTAAAATGTCCTTATTTGTTATTAAATTCACATGTGATAAACCAAAGGTCACAACTGCTTTAATAGTTTATGGTGTTTTCCAACAAGGGTAAACTTGAGAAAACAATAATAAATTCACTAAAACATCAAAAGTTATGGAATATAATTGAAACTCTAAAACATCAAAAGAAAAGGAATGGAGGGAGTAATAACTAAACCATATACTCCCTTAATATGAGAGTAGTTTACAAAAATTACAGGTATTAAGAAAAATTGAGTAATAAATTTGTCTGAAAAATATGTGACTATTACTAAACTGCCCTTTTATGTATAAATGCATTCTATGTTGATTTTTCATATTTTAAGACAATTGATAGTATTGATAAAGGGTATAGTTGGAAAAAAAAATAATGAATGCATTAGAAATTTATAGATGGTCTTCTATTTAAGGACAAACACTTTTTTGTAAATGGTCTTATAATTAGGGAAGGAGGGAGTGATACATTTATATTGATAATAATAACAGAGGCTCCGTTCAAAAAACAGAGGCTCATTTTTTTGTCTGTCTGATTCTATACATGTATAGAAGAAATGGCATCAGCAATATTTTTTATCAAAAAAAAAGTTGTAGCATCATCAAGCTTTAAATTAGTGTTTAATGATAATGGTATGTGATTTTGCAACTCATAGTTCATGTTTAGGAGGGTAAATTTCCAATATGAGAATCAAAGGAAATAAGGAATGAACATGCATGATTAGGGGTTTGATTTGAAGTTTAGAACATAAAACAATGAAACTTTAAGTTATTTTCATACCTAAACATCTAGGCAGGAATTGCTGCTCATGATTAGTGTTAAATTTTACATTATCAATAAACCAGTGTCATGTACTTACTTAGTAATTGATTCCCGAGTCCTTTCCAGGTTTTGCCGTTTCTTCTTTTGAGTGCAGCACCCTTTTGTTTGTTGATACTGTAAAACTCTTTTACTTTGATAATAGGTCCATCTTAAATGTTCATTTTTGAAATGTCTGTTATTTGTTCATATTTTTCATTCAGTTTTCTTGGCATCCCAACTTGGTGGCCCTTATGTTTTTTAACAGTATTTTGAAAATGGCTCTTATGTTTTTGAAGGGTGTTTTGAAAATGATTATAATTTCTAGATAAGGGTTCTCATCTTACTCAAAATCTTAGCAGCAGCCAGCAGCGCCTATAGCACCACGTCCAGCTCCTTGCTAGTGTTTGTTTCTTCTTCCTTCTCCACTCCTGCCAAAACCAAGTTCCTACCCTCACTTTCTAGATTTTCTGTCAAGCGTCCTTGCTTGTTTTCACCAACCCGTCCCCATTTTACTGTAACCAAGCCCTCCATGAACCTTCTGAACAAACTTGGATTTGGCAGTGCAAGGTCATCGGAGAGCATGGATTCAACCATTCCTCAGGGACCAGATGACGATATACCAGCACCAGGTCAGCAGTTTGCTGCTTTTGGGGCTGGCTGCTTCTGGGGTGTTGAATTAGTCTTCCAGAGGGTGCCTGGAGTTAGTAAGACAGAGGTTGGTTACACCCAGGGGCTTTTGCACAATCCTACTTATGAAGATGTGTGTTCAGGGACCACAAACCACAATGAGGTTGTAAGGGTCCAATATGATCCCAAACAATGTGCCTTTGAGAATCTGCTTGATACGTTCTGGTCGAAACATGATCCTACTACGCCAAATCGACAGGTATATACCGAATTGTTCGTTTGTTTATAAACTTAATAATGCTAAGTTGAGTTCCTCTTGTTTGCGAATTGAGTTCTTTTTTGTCTCAAGTGGCATGTCCTGACTTTGCTGATCTGAATAATAAGTCTAGACTTGTGGATTGTCAACATTGATGGTTTTTTTATTTATGTTACTTTGTGTTTCTAACATTTTTTAAATGATGAATTGCCTCCAATGTTAATCATTTTGATAGCAATTTTCTTATTCTGTGATTAATTCGTCTGAAACATTATTGATTCTCTCTGTTGCAGTATCTTACAAATGCATTCAGTTTCTATATCTAATCATGTCATGTTTTGTAAATTATGTTCCAACATGTACTTTTAGTAAATTTGGGTCAAATCAAATTCCCAGGAAAAATGATTGTCTTGTCACTGAGTATGTGATGGATCTCTCAAAAAGAACAACTCATGAGCATCTAGAAAAGTATAAAACCATTTTGATATGGAGTAATCTATTTTCCTTACCTAACATTTTGTCTTGTCCCTTTGTTTCTTTTCTGTTCAAGACTGTGGTTTCTTTTGTTCTTTCATTTTCTTATGCATGCTTTTGTTTCTGTAGTTCTCTTCTCTCTTTCGTTCAATCTATTCGAATGCATGAACTTTTGAATATAAGATAGTTGAGTAGTGATGCTTGAGTGCTGATTTCCACTTTCCATGAATGGTAAGAGCTTAGTAATTGTGACATCTGGATCTTTGTATGTGACAGGGGAATGATGTGGGAACACAGTACAGATCTGGAATATACTTTTACACTCCTGAGCAAGAGAAAATAGCTAAAGAGTCTTTGGAACAACAAGAGAAGCAATTGGGCAGGAAGATTGCTACTGAGATTCTTCCTGCCAAGAAGTTCTACAGGGCTGAGGAGTACCATCAACAGTACCTTGAGAAAGGTGGTCGATTCGGTTTCAAGCAATCTGCTGCTAAAGGATGCAATGATCCAATTCGGTGCTATGGTTAA
Protein sequence

>MS.gene065502.t1

MRICGAAASSAYSTTSSSLLVFVSSSFSTPAKTKFLPSLSRFSVKRPCLFSPTRPHFTVTKPSMNLLNKLGFGSARSSESMDSTIPQGPDDDIPAPGQQFAAFGAGCFWGVELVFQRVPGVSKTEVGYTQGLLHNPTYEDVCSGTTNHNEVVRVQYDPKQCAFENLLDTFWSKHDPTTPNRQGNDVGTQYRSGIYFYTPEQEKIAKESLEQQEKQLGRKIATEILPAKKFYRAEEYHQQYLEKGGRFGFKQSAAKGCNDPIRCYG