Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28113.t1 | XP_003630070.1 | 98.6 | 213 | 3 | 0 | 1 | 213 | 1 | 213 | 2.00E-119 | 438.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28113.t1 | Q9SCY3 | 76.5 | 170 | 40 | 0 | 44 | 213 | 48 | 217 | 2.3e-76 | 286.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28113.t1 | G7LAE7 | 98.6 | 213 | 3 | 0 | 1 | 213 | 1 | 213 | 1.4e-119 | 438.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene28113 | 0.817162 | 3.81E-52 | -1.69E-46 |
| MS.gene049346 | MS.gene28113 | 0.813938 | 1.99E-51 | -1.69E-46 |
| MS.gene049427 | MS.gene28113 | 0.82006 | 8.37E-53 | -1.69E-46 |
| MS.gene049488 | MS.gene28113 | 0.828402 | 9.17E-55 | -1.69E-46 |
| MS.gene049609 | MS.gene28113 | 0.805658 | 1.20E-49 | -1.69E-46 |
| MS.gene049661 | MS.gene28113 | 0.82451 | 7.76E-54 | -1.69E-46 |
| MS.gene049662 | MS.gene28113 | 0.816314 | 5.89E-52 | -1.69E-46 |
| MS.gene049805 | MS.gene28113 | 0.839111 | 1.93E-57 | -1.69E-46 |
| MS.gene049910 | MS.gene28113 | 0.83158 | 1.54E-55 | -1.69E-46 |
| MS.gene049993 | MS.gene28113 | 0.814227 | 1.72E-51 | -1.69E-46 |
| MS.gene050076 | MS.gene28113 | 0.823296 | 1.49E-53 | -1.69E-46 |
| MS.gene050301 | MS.gene28113 | 0.849196 | 3.78E-60 | -1.69E-46 |
| MS.gene050397 | MS.gene28113 | 0.823839 | 1.12E-53 | -1.69E-46 |
| MS.gene050406 | MS.gene28113 | 0.815073 | 1.11E-51 | -1.69E-46 |
| MS.gene050432 | MS.gene28113 | 0.820843 | 5.54E-53 | -1.69E-46 |
| MS.gene050464 | MS.gene28113 | 0.83636 | 9.80E-57 | -1.69E-46 |
| MS.gene050529 | MS.gene28113 | 0.823525 | 1.32E-53 | -1.69E-46 |
| MS.gene050593 | MS.gene28113 | 0.847994 | 8.13E-60 | -1.69E-46 |
| MS.gene050670 | MS.gene28113 | 0.88725 | 1.68E-72 | -1.69E-46 |
| MS.gene050747 | MS.gene28113 | 0.803289 | 3.74E-49 | -1.69E-46 |
| MS.gene050783 | MS.gene28113 | 0.814739 | 1.32E-51 | -1.69E-46 |
| MS.gene050812 | MS.gene28113 | 0.816763 | 4.68E-52 | -1.69E-46 |
| MS.gene05101 | MS.gene28113 | 0.841635 | 4.22E-58 | -1.69E-46 |
| MS.gene05105 | MS.gene28113 | 0.858826 | 6.31E-63 | -1.69E-46 |
| MS.gene051387 | MS.gene28113 | 0.865121 | 7.43E-65 | -1.69E-46 |
| MS.gene051525 | MS.gene28113 | 0.89465 | 2.02E-75 | -1.69E-46 |
| MS.gene051582 | MS.gene28113 | 0.901159 | 3.55E-78 | -1.69E-46 |
| MS.gene051588 | MS.gene28113 | 0.828435 | 9.00E-55 | -1.69E-46 |
| MS.gene051605 | MS.gene28113 | 0.810641 | 1.04E-50 | -1.69E-46 |
| MS.gene051860 | MS.gene28113 | 0.801809 | 7.56E-49 | -1.69E-46 |
| MS.gene052011 | MS.gene28113 | -0.844195 | 8.79E-59 | -1.69E-46 |
| MS.gene052077 | MS.gene28113 | 0.80895 | 2.41E-50 | -1.69E-46 |
| MS.gene052128 | MS.gene28113 | -0.801222 | 9.97E-49 | -1.69E-46 |
| MS.gene052135 | MS.gene28113 | 0.804543 | 2.05E-49 | -1.69E-46 |
| MS.gene052188 | MS.gene28113 | 0.844138 | 9.10E-59 | -1.69E-46 |
| MS.gene052253 | MS.gene28113 | 0.865938 | 4.11E-65 | -1.69E-46 |
| MS.gene052310 | MS.gene28113 | 0.85271 | 3.86E-61 | -1.69E-46 |
| MS.gene052322 | MS.gene28113 | 0.820241 | 7.61E-53 | -1.69E-46 |
| MS.gene052340 | MS.gene28113 | 0.856642 | 2.80E-62 | -1.69E-46 |
| MS.gene052414 | MS.gene28113 | 0.819631 | 1.05E-52 | -1.69E-46 |
| MS.gene052421 | MS.gene28113 | 0.869395 | 3.20E-66 | -1.69E-46 |
| MS.gene052481 | MS.gene28113 | 0.867497 | 1.31E-65 | -1.69E-46 |
| MS.gene052484 | MS.gene28113 | 0.808066 | 3.72E-50 | -1.69E-46 |
| MS.gene052485 | MS.gene28113 | 0.858048 | 1.08E-62 | -1.69E-46 |
| MS.gene052501 | MS.gene28113 | 0.846863 | 1.66E-59 | -1.69E-46 |
| MS.gene052505 | MS.gene28113 | 0.858301 | 9.05E-63 | -1.69E-46 |
| MS.gene052615 | MS.gene28113 | 0.803847 | 2.87E-49 | -1.69E-46 |
| MS.gene052982 | MS.gene28113 | 0.842112 | 3.15E-58 | -1.69E-46 |
| MS.gene053103 | MS.gene28113 | 0.817389 | 3.38E-52 | -1.69E-46 |
| MS.gene053209 | MS.gene28113 | 0.823267 | 1.52E-53 | -1.69E-46 |
| MS.gene053322 | MS.gene28113 | 0.800878 | 1.17E-48 | -1.69E-46 |
| MS.gene053330 | MS.gene28113 | 0.809497 | 1.84E-50 | -1.69E-46 |
| MS.gene053557 | MS.gene28113 | 0.80234 | 5.88E-49 | -1.69E-46 |
| MS.gene053579 | MS.gene28113 | 0.805452 | 1.33E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene28113 | 0.840795 | 7.01E-58 | -1.69E-46 |
| MS.gene053873 | MS.gene28113 | 0.813482 | 2.50E-51 | -1.69E-46 |
| MS.gene053928 | MS.gene28113 | 0.806016 | 1.01E-49 | -1.69E-46 |
| MS.gene054102 | MS.gene28113 | 0.830751 | 2.46E-55 | -1.69E-46 |
| MS.gene054110 | MS.gene28113 | 0.806677 | 7.32E-50 | -1.69E-46 |
| MS.gene054123 | MS.gene28113 | 0.817379 | 3.40E-52 | -1.69E-46 |
| MS.gene05412 | MS.gene28113 | 0.803507 | 3.37E-49 | -1.69E-46 |
| MS.gene05413 | MS.gene28113 | 0.854889 | 9.11E-62 | -1.69E-46 |
| MS.gene054183 | MS.gene28113 | 0.835315 | 1.80E-56 | -1.69E-46 |
| MS.gene054349 | MS.gene28113 | 0.821636 | 3.63E-53 | -1.69E-46 |
| MS.gene054719 | MS.gene28113 | 0.847271 | 1.29E-59 | -1.69E-46 |
| MS.gene054917 | MS.gene28113 | 0.81967 | 1.03E-52 | -1.69E-46 |
| MS.gene054918 | MS.gene28113 | 0.825866 | 3.71E-54 | -1.69E-46 |
| MS.gene054920 | MS.gene28113 | 0.814106 | 1.82E-51 | -1.69E-46 |
| MS.gene054927 | MS.gene28113 | 0.815057 | 1.12E-51 | -1.69E-46 |
| MS.gene05505 | MS.gene28113 | 0.808333 | 3.26E-50 | -1.69E-46 |
| MS.gene055116 | MS.gene28113 | 0.887968 | 8.95E-73 | -1.69E-46 |
| MS.gene055277 | MS.gene28113 | 0.828174 | 1.04E-54 | -1.69E-46 |
| MS.gene055369 | MS.gene28113 | 0.860059 | 2.69E-63 | -1.69E-46 |
| MS.gene055412 | MS.gene28113 | 0.824978 | 6.02E-54 | -1.69E-46 |
| MS.gene055422 | MS.gene28113 | 0.806377 | 8.47E-50 | -1.69E-46 |
| MS.gene055428 | MS.gene28113 | 0.826107 | 3.25E-54 | -1.69E-46 |
| MS.gene05553 | MS.gene28113 | 0.865062 | 7.75E-65 | -1.69E-46 |
| MS.gene055564 | MS.gene28113 | 0.812669 | 3.77E-51 | -1.69E-46 |
| MS.gene055911 | MS.gene28113 | 0.804394 | 2.21E-49 | -1.69E-46 |
| MS.gene05594 | MS.gene28113 | 0.82974 | 4.35E-55 | -1.69E-46 |
| MS.gene056191 | MS.gene28113 | 0.859189 | 4.91E-63 | -1.69E-46 |
| MS.gene056337 | MS.gene28113 | 0.817528 | 3.15E-52 | -1.69E-46 |
| MS.gene056407 | MS.gene28113 | 0.871642 | 5.85E-67 | -1.69E-46 |
| MS.gene056410 | MS.gene28113 | 0.807112 | 5.93E-50 | -1.69E-46 |
| MS.gene056451 | MS.gene28113 | 0.81974 | 9.91E-53 | -1.69E-46 |
| MS.gene056497 | MS.gene28113 | 0.802784 | 4.76E-49 | -1.69E-46 |
| MS.gene056511 | MS.gene28113 | 0.847071 | 1.46E-59 | -1.69E-46 |
| MS.gene056514 | MS.gene28113 | 0.855744 | 5.14E-62 | -1.69E-46 |
| MS.gene056612 | MS.gene28113 | 0.810022 | 1.42E-50 | -1.69E-46 |
| MS.gene056659 | MS.gene28113 | 0.801323 | 9.51E-49 | -1.69E-46 |
| MS.gene056723 | MS.gene28113 | 0.824978 | 6.02E-54 | -1.69E-46 |
| MS.gene056899 | MS.gene28113 | -0.819473 | 1.14E-52 | -1.69E-46 |
| MS.gene057011 | MS.gene28113 | 0.820474 | 6.73E-53 | -1.69E-46 |
| MS.gene05702 | MS.gene28113 | 0.866996 | 1.89E-65 | -1.69E-46 |
| MS.gene057123 | MS.gene28113 | 0.81237 | 4.39E-51 | -1.69E-46 |
| MS.gene057124 | MS.gene28113 | 0.839945 | 1.17E-57 | -1.69E-46 |
| MS.gene057146 | MS.gene28113 | 0.844312 | 8.17E-59 | -1.69E-46 |
| MS.gene057416 | MS.gene28113 | 0.834321 | 3.21E-56 | -1.69E-46 |
| MS.gene057448 | MS.gene28113 | 0.815342 | 9.71E-52 | -1.69E-46 |
| MS.gene057450 | MS.gene28113 | 0.813937 | 1.99E-51 | -1.69E-46 |
| MS.gene057476 | MS.gene28113 | 0.815194 | 1.05E-51 | -1.69E-46 |
| MS.gene057749 | MS.gene28113 | 0.879518 | 1.17E-69 | -1.69E-46 |
| MS.gene057823 | MS.gene28113 | 0.870968 | 9.77E-67 | -1.69E-46 |
| MS.gene057825 | MS.gene28113 | 0.864201 | 1.44E-64 | -1.69E-46 |
| MS.gene057888 | MS.gene28113 | 0.823878 | 1.09E-53 | -1.69E-46 |
| MS.gene058002 | MS.gene28113 | 0.855785 | 5.00E-62 | -1.69E-46 |
| MS.gene058007 | MS.gene28113 | 0.844285 | 8.31E-59 | -1.69E-46 |
| MS.gene058019 | MS.gene28113 | 0.858027 | 1.09E-62 | -1.69E-46 |
| MS.gene05811 | MS.gene28113 | 0.815375 | 9.55E-52 | -1.69E-46 |
| MS.gene058142 | MS.gene28113 | 0.817129 | 3.87E-52 | -1.69E-46 |
| MS.gene058233 | MS.gene28113 | 0.854219 | 1.42E-61 | -1.69E-46 |
| MS.gene058276 | MS.gene28113 | 0.806378 | 8.47E-50 | -1.69E-46 |
| MS.gene058293 | MS.gene28113 | 0.830074 | 3.60E-55 | -1.69E-46 |
| MS.gene058444 | MS.gene28113 | 0.851014 | 1.17E-60 | -1.69E-46 |
| MS.gene058660 | MS.gene28113 | 0.850879 | 1.28E-60 | -1.69E-46 |
| MS.gene058700 | MS.gene28113 | 0.863099 | 3.17E-64 | -1.69E-46 |
| MS.gene05886 | MS.gene28113 | 0.854547 | 1.14E-61 | -1.69E-46 |
| MS.gene058928 | MS.gene28113 | 0.835463 | 1.65E-56 | -1.69E-46 |
| MS.gene058995 | MS.gene28113 | 0.846056 | 2.76E-59 | -1.69E-46 |
| MS.gene059011 | MS.gene28113 | 0.833335 | 5.66E-56 | -1.69E-46 |
| MS.gene05907 | MS.gene28113 | 0.8663 | 3.15E-65 | -1.69E-46 |
| MS.gene059082 | MS.gene28113 | 0.825905 | 3.63E-54 | -1.69E-46 |
| MS.gene059141 | MS.gene28113 | 0.872149 | 3.97E-67 | -1.69E-46 |
| MS.gene059420 | MS.gene28113 | 0.871357 | 7.27E-67 | -1.69E-46 |
| MS.gene059455 | MS.gene28113 | 0.814288 | 1.66E-51 | -1.69E-46 |
| MS.gene059462 | MS.gene28113 | 0.841544 | 4.46E-58 | -1.69E-46 |
| MS.gene059536 | MS.gene28113 | 0.829038 | 6.44E-55 | -1.69E-46 |
| MS.gene059597 | MS.gene28113 | 0.855846 | 4.80E-62 | -1.69E-46 |
| MS.gene059676 | MS.gene28113 | 0.843861 | 1.08E-58 | -1.69E-46 |
| MS.gene059688 | MS.gene28113 | 0.900351 | 8.00E-78 | -1.69E-46 |
| MS.gene059692 | MS.gene28113 | 0.89435 | 2.68E-75 | -1.69E-46 |
| MS.gene059695 | MS.gene28113 | 0.896877 | 2.42E-76 | -1.69E-46 |
| MS.gene059807 | MS.gene28113 | 0.806869 | 6.67E-50 | -1.69E-46 |
| MS.gene05984 | MS.gene28113 | 0.801757 | 7.74E-49 | -1.69E-46 |
| MS.gene059894 | MS.gene28113 | 0.811457 | 6.94E-51 | -1.69E-46 |
| MS.gene059960 | MS.gene28113 | 0.806684 | 7.30E-50 | -1.69E-46 |
| MS.gene060247 | MS.gene28113 | 0.825382 | 4.83E-54 | -1.69E-46 |
| MS.gene060403 | MS.gene28113 | 0.88827 | 6.85E-73 | -1.69E-46 |
| MS.gene060407 | MS.gene28113 | 0.819411 | 1.18E-52 | -1.69E-46 |
| MS.gene060804 | MS.gene28113 | 0.810342 | 1.21E-50 | -1.69E-46 |
| MS.gene060835 | MS.gene28113 | 0.81949 | 1.13E-52 | -1.69E-46 |
| MS.gene060837 | MS.gene28113 | 0.815518 | 8.87E-52 | -1.69E-46 |
| MS.gene060986 | MS.gene28113 | 0.802998 | 4.30E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene28113 | 0.876489 | 1.34E-68 | -1.69E-46 |
| MS.gene061051 | MS.gene28113 | -0.815914 | 7.24E-52 | -1.69E-46 |
| MS.gene061154 | MS.gene28113 | 0.84772 | 9.67E-60 | -1.69E-46 |
| MS.gene061159 | MS.gene28113 | 0.848215 | 7.07E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28113.t1 | MTR_8g091410 | 98.592 | 213 | 3 | 0 | 1 | 213 | 1 | 213 | 2.04e-155 | 429 |
| MS.gene28113.t1 | MTR_4g094785 | 53.846 | 130 | 59 | 1 | 76 | 204 | 108 | 237 | 1.41e-42 | 144 |
| MS.gene28113.t1 | MTR_8g012380 | 40.000 | 110 | 55 | 2 | 104 | 213 | 46 | 144 | 1.40e-19 | 82.0 |
| MS.gene28113.t1 | MTR_4g068190 | 28.090 | 178 | 111 | 4 | 36 | 203 | 48 | 218 | 6.45e-17 | 76.6 |
| MS.gene28113.t1 | MTR_7g053470 | 38.235 | 102 | 50 | 3 | 108 | 208 | 52 | 141 | 1.12e-12 | 66.6 |
| MS.gene28113.t1 | MTR_8g080980 | 36.885 | 122 | 63 | 4 | 89 | 208 | 36 | 145 | 1.90e-12 | 65.9 |
| MS.gene28113.t1 | MTR_8g012340 | 36.449 | 107 | 55 | 3 | 103 | 208 | 53 | 147 | 1.07e-11 | 63.9 |
| MS.gene28113.t1 | MTR_1g061850 | 35.075 | 134 | 66 | 5 | 81 | 206 | 123 | 243 | 1.73e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28113.t1 | AT4G39710 | 76.471 | 170 | 40 | 0 | 44 | 213 | 48 | 217 | 1.07e-94 | 275 |
| MS.gene28113.t1 | AT4G39710 | 76.471 | 170 | 40 | 0 | 44 | 213 | 48 | 217 | 1.07e-94 | 275 |
| MS.gene28113.t1 | AT4G39710 | 75.630 | 119 | 29 | 0 | 44 | 162 | 48 | 166 | 2.19e-62 | 192 |
| MS.gene28113.t1 | AT5G45680 | 56.911 | 123 | 53 | 0 | 83 | 205 | 84 | 206 | 6.31e-44 | 146 |
| MS.gene28113.t1 | AT5G48580 | 39.806 | 103 | 51 | 2 | 104 | 206 | 48 | 139 | 1.61e-18 | 79.3 |
| MS.gene28113.t1 | AT3G25220 | 40.777 | 103 | 50 | 2 | 104 | 206 | 48 | 139 | 1.01e-17 | 77.0 |
| MS.gene28113.t1 | AT2G43560 | 32.317 | 164 | 85 | 5 | 49 | 203 | 66 | 212 | 1.12e-15 | 73.2 |
| MS.gene28113.t1 | AT2G43560 | 32.317 | 164 | 85 | 5 | 49 | 203 | 10 | 156 | 1.31e-15 | 72.0 |
| MS.gene28113.t1 | AT5G05420 | 37.736 | 106 | 52 | 4 | 102 | 206 | 50 | 142 | 1.18e-14 | 68.6 |
| MS.gene28113.t1 | AT3G25230 | 37.008 | 127 | 66 | 4 | 84 | 208 | 32 | 146 | 3.11e-13 | 68.2 |
| MS.gene28113.t1 | AT3G25230 | 37.008 | 127 | 66 | 4 | 84 | 208 | 32 | 146 | 3.25e-13 | 68.2 |
| MS.gene28113.t1 | AT5G48570 | 36.634 | 101 | 53 | 2 | 108 | 208 | 65 | 154 | 1.24e-11 | 63.5 |
| MS.gene28113.t1 | AT3G10060 | 30.000 | 160 | 91 | 5 | 55 | 206 | 70 | 216 | 2.51e-11 | 61.2 |
Find 62 sgRNAs with CRISPR-Local
Find 95 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGATTGGATCAGGGAATTT+TGG | 0.124572 | 4.4:-10071428 | MS.gene28113:CDS |
| AATGTGAATTTCAGGTAATT+AGG | 0.235234 | 4.4:-10071450 | MS.gene28113:intron |
| TAAATTTGTTGGTCTTTATT+CGG | 0.242676 | 4.4:-10070780 | MS.gene28113:CDS |
| TGCTGAGCATAACACCTTAT+TGG | 0.271308 | 4.4:+10071953 | None:intergenic |
| TGTGATTATGTTGAGGGTTT+TGG | 0.277118 | 4.4:-10071762 | MS.gene28113:CDS |
| GGTAATTAGGGGATTGGATC+AGG | 0.291509 | 4.4:-10071437 | MS.gene28113:CDS |
| GAGCTTAATTATGTTAAATC+TGG | 0.297049 | 4.4:-10071795 | MS.gene28113:CDS |
| AAATTTGTTGGTCTTTATTC+GGG | 0.311128 | 4.4:-10070779 | MS.gene28113:CDS |
| CTGTATGATATTAAATTTGT+TGG | 0.318710 | 4.4:-10070791 | MS.gene28113:CDS |
| CGTTAGTTGGAATTGGTATT+GGG | 0.335154 | 4.4:-10071908 | MS.gene28113:CDS |
| ATGTGAATTTCAGGTAATTA+GGG | 0.335286 | 4.4:-10071449 | MS.gene28113:intron |
| GAACCTGCAGGCTGCTTCTC+AGG | 0.336346 | 4.4:-10071251 | MS.gene28113:intron |
| TTTGGTGATGAAGCTCCTTT+AGG | 0.338411 | 4.4:-10071744 | MS.gene28113:CDS |
| GAGAAGCAGCCTGCAGGTTC+AGG | 0.345343 | 4.4:+10071254 | None:intergenic |
| CTCTCATTCTCACTCTGTTC+AGG | 0.375218 | 4.4:-10072038 | MS.gene28113:CDS |
| AAGAAGCCGTTAGTTGGAAT+TGG | 0.375819 | 4.4:-10071915 | MS.gene28113:CDS |
| CCGTTAGTTGGAATTGGTAT+TGG | 0.376392 | 4.4:-10071909 | MS.gene28113:CDS |
| GGTTATTGTGATTATGTTGA+GGG | 0.390983 | 4.4:-10071768 | MS.gene28113:CDS |
| CCTTCTTGTATGAAATTGAA+TGG | 0.401384 | 4.4:-10071993 | MS.gene28113:CDS |
| AATTATGTTAAATCTGGGCT+TGG | 0.422396 | 4.4:-10071789 | MS.gene28113:CDS |
| ATTGTAGCAGCATGGGTAAT+GGG | 0.427828 | 4.4:-10071885 | MS.gene28113:CDS |
| ATTTCAGGTAATTAGGGGAT+TGG | 0.431931 | 4.4:-10071443 | MS.gene28113:intron |
| AACGAAAAGAAGCCGTTAGT+TGG | 0.432641 | 4.4:-10071921 | MS.gene28113:CDS |
| GGGATTAACGGCGTTGGATG+CGG | 0.454068 | 4.4:-10071865 | MS.gene28113:CDS |
| AGCTTAATTATGTTAAATCT+GGG | 0.462974 | 4.4:-10071794 | MS.gene28113:CDS |
| AAGTTATAAAGTATCCAATA+AGG | 0.464158 | 4.4:-10071967 | MS.gene28113:CDS |
| AGCATGGGTAATGGGATTAA+CGG | 0.477643 | 4.4:-10071877 | MS.gene28113:CDS |
| AATAGGTGACTGCAATATAC+CGG | 0.483379 | 4.4:-10070828 | MS.gene28113:intron |
| ATTTCATACAAGAAGGAAAA+CGG | 0.483988 | 4.4:+10072000 | None:intergenic |
| CCATTCAATTTCATACAAGA+AGG | 0.487470 | 4.4:+10071993 | None:intergenic |
| GATTGTAGCAGCATGGGTAA+TGG | 0.497305 | 4.4:-10071886 | MS.gene28113:CDS |
| TGGTTATTGTGATTATGTTG+AGG | 0.508666 | 4.4:-10071769 | MS.gene28113:CDS |
| AATTTAATACCTATCCGCAT+TGG | 0.515893 | 4.4:+10071388 | None:intergenic |
| ATTCCTCCATTGTTAGCGTA+TGG | 0.515896 | 4.4:-10071278 | MS.gene28113:CDS |
| AAACCTGAGAAGCAGCCTGC+AGG | 0.532161 | 4.4:+10071248 | None:intergenic |
| ATAGTAGTTATAAACGTGCT+AGG | 0.536904 | 4.4:-10071576 | MS.gene28113:CDS |
| TTAATACCTATCCGCATTGG+AGG | 0.539094 | 4.4:+10071391 | None:intergenic |
| TACAGAAGAGTTGCATTGCC+CGG | 0.539306 | 4.4:+10070809 | None:intergenic |
| GGTAATGGGATTAACGGCGT+TGG | 0.544791 | 4.4:-10071871 | MS.gene28113:CDS |
| AGGCCACTCACTATGCGTAT+TGG | 0.545910 | 4.4:-10071556 | MS.gene28113:CDS |
| ACATCAATAAGCTCTCCTAA+AGG | 0.553954 | 4.4:+10071729 | None:intergenic |
| GCGTATGGTCCTGAACCTGC+AGG | 0.559980 | 4.4:-10071263 | MS.gene28113:CDS |
| CTCACTATGCGTATTGGTGT+TGG | 0.565060 | 4.4:-10071550 | MS.gene28113:CDS |
| TATTGGGATTGTAGCAGCAT+GGG | 0.571835 | 4.4:-10071892 | MS.gene28113:CDS |
| AATTGAATACTATGCTACTG+TGG | 0.592481 | 4.4:-10071832 | MS.gene28113:CDS |
| GTAATTAGGGGATTGGATCA+GGG | 0.593610 | 4.4:-10071436 | MS.gene28113:CDS |
| ACTATCAAATACTATTCCAT+CGG | 0.594477 | 4.4:+10071594 | None:intergenic |
| ATTGAATACTATGCTACTGT+GGG | 0.611936 | 4.4:-10071831 | MS.gene28113:CDS |
| CCAATACCAATTCCAACTAA+CGG | 0.620051 | 4.4:+10071909 | None:intergenic |
| TATGCGTATTGGTGTTGGCA+AGG | 0.622438 | 4.4:-10071545 | MS.gene28113:intron |
| GTATTGGGATTGTAGCAGCA+TGG | 0.623097 | 4.4:-10071893 | MS.gene28113:CDS |
| TGTGAATTTCAGGTAATTAG+GGG | 0.623369 | 4.4:-10071448 | MS.gene28113:intron |
| ACATAATTAAGCTCACACAT+TGG | 0.625130 | 4.4:+10071804 | None:intergenic |
| GGCGTACCTCCAATGCGGAT+AGG | 0.639198 | 4.4:-10071397 | MS.gene28113:intron |
| AGAAGGAAAACGGTTATGAA+CGG | 0.643601 | 4.4:+10072010 | None:intergenic |
| GTGTTATGCTCAGCACGTAA+CGG | 0.647081 | 4.4:-10071945 | MS.gene28113:CDS |
| ATAGGTGACTGCAATATACC+GGG | 0.650180 | 4.4:-10070827 | MS.gene28113:intron |
| TCAGGACCATACGCTAACAA+TGG | 0.656714 | 4.4:+10071272 | None:intergenic |
| GGACCATACGCTAACAATGG+AGG | 0.666829 | 4.4:+10071275 | None:intergenic |
| ACACCAATACGCATAGTGAG+TGG | 0.668322 | 4.4:+10071553 | None:intergenic |
| TATACTGCTAGATTCGCCGA+TGG | 0.684653 | 4.4:-10071610 | MS.gene28113:CDS |
| GTGAAGGCGTACCTCCAATG+CGG | 0.724987 | 4.4:-10071402 | MS.gene28113:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTATTTACTATTTTTACTT+AGG | - | chr4.4:10071487-10071506 | MS.gene28113:intron | 10.0% |
| !! | AATCAAATTAATCGTGTATT+TGG | - | chr4.4:10071643-10071662 | MS.gene28113:intron | 20.0% |
| !! | AGCTTAATTATGTTAAATCT+GGG | - | chr4.4:10071013-10071032 | MS.gene28113:intron | 20.0% |
| !! | ATTATTATCAGAAGAAGAAA+AGG | - | chr4.4:10071911-10071930 | MS.gene28113:CDS | 20.0% |
| !! | CTGTATGATATTAAATTTGT+TGG | - | chr4.4:10072016-10072035 | MS.gene28113:CDS | 20.0% |
| !! | TAAATCTAACATATTTGATG+CGG | - | chr4.4:10071441-10071460 | MS.gene28113:intron | 20.0% |
| !!! | AAGTTATAAAGTATCCAATA+AGG | - | chr4.4:10070840-10070859 | MS.gene28113:intron | 20.0% |
| !!! | AGTTTTATCATTGATTCAAT+AGG | - | chr4.4:10071962-10071981 | MS.gene28113:CDS | 20.0% |
| !!! | ATTTACTATTTTTACTTAGG+TGG | - | chr4.4:10071490-10071509 | MS.gene28113:intron | 20.0% |
| !!! | TAAATTTGTTGGTCTTTATT+CGG | - | chr4.4:10072027-10072046 | MS.gene28113:CDS | 20.0% |
| !!! | TTTACTATTTTTACTTAGGT+GGG | - | chr4.4:10071491-10071510 | MS.gene28113:intron | 20.0% |
| ! | ACTATCAAATACTATTCCAT+CGG | + | chr4.4:10071216-10071235 | None:intergenic | 25.0% |
| ! | ATTTCATACAAGAAGGAAAA+CGG | + | chr4.4:10070810-10070829 | None:intergenic | 25.0% |
| ! | GAGCTTAATTATGTTAAATC+TGG | - | chr4.4:10071012-10071031 | MS.gene28113:intron | 25.0% |
| ! | TACATTGGTAGAAAATATAC+CGG | + | chr4.4:10071693-10071712 | None:intergenic | 25.0% |
| !! | AAAAGACCATGGATTTTATT+AGG | - | chr4.4:10071863-10071882 | MS.gene28113:CDS | 25.0% |
| !! | AATGTGAATTTCAGGTAATT+AGG | - | chr4.4:10071357-10071376 | MS.gene28113:intron | 25.0% |
| !! | ATGTGAATTTCAGGTAATTA+GGG | - | chr4.4:10071358-10071377 | MS.gene28113:intron | 25.0% |
| !!! | AAATTTGTTGGTCTTTATTC+GGG | - | chr4.4:10072028-10072047 | MS.gene28113:CDS | 25.0% |
| !!! | TTTCATGTTTTTGTTGAGTT+TGG | - | chr4.4:10071321-10071340 | MS.gene28113:intron | 25.0% |
| AATTATGTTAAATCTGGGCT+TGG | - | chr4.4:10071018-10071037 | MS.gene28113:intron | 30.0% | |
| AATTGAATACTATGCTACTG+TGG | - | chr4.4:10070975-10070994 | MS.gene28113:intron | 30.0% | |
| AATTTAATACCTATCCGCAT+TGG | + | chr4.4:10071422-10071441 | None:intergenic | 30.0% | |
| ACATAATTAAGCTCACACAT+TGG | + | chr4.4:10071006-10071025 | None:intergenic | 30.0% | |
| ACATTGGTAGAAAATATACC+GGG | + | chr4.4:10071692-10071711 | None:intergenic | 30.0% | |
| ATAGTAGTTATAAACGTGCT+AGG | - | chr4.4:10071231-10071250 | MS.gene28113:intron | 30.0% | |
| ATCTACAATTGAAAAGACCA+TGG | - | chr4.4:10071852-10071871 | MS.gene28113:CDS | 30.0% | |
| ATTGAATACTATGCTACTGT+GGG | - | chr4.4:10070976-10070995 | MS.gene28113:intron | 30.0% | |
| CCATTCAATTTCATACAAGA+AGG | + | chr4.4:10070817-10070836 | None:intergenic | 30.0% | |
| CCTTCTTGTATGAAATTGAA+TGG | - | chr4.4:10070814-10070833 | MS.gene28113:CDS | 30.0% | |
| GGTTATTGTGATTATGTTGA+GGG | - | chr4.4:10071039-10071058 | MS.gene28113:intron | 30.0% | |
| TGGTTATTGTGATTATGTTG+AGG | - | chr4.4:10071038-10071057 | MS.gene28113:intron | 30.0% | |
| TGTTGAGTTTGGACTTATAA+GGG | - | chr4.4:10071332-10071351 | MS.gene28113:intron | 30.0% | |
| TTGTTGAGTTTGGACTTATA+AGG | - | chr4.4:10071331-10071350 | MS.gene28113:intron | 30.0% | |
| ! | GTCTTTTCAATTGTAGATTG+CGG | + | chr4.4:10071850-10071869 | None:intergenic | 30.0% |
| ! | TGTGAATTTCAGGTAATTAG+GGG | - | chr4.4:10071359-10071378 | MS.gene28113:intron | 30.0% |
| !!! | TGTAGCTGCTTTTTTTACAT+TGG | + | chr4.4:10071708-10071727 | None:intergenic | 30.0% |
| AATAGGTGACTGCAATATAC+CGG | - | chr4.4:10071979-10071998 | MS.gene28113:CDS | 35.0% | |
| ACATCAATAAGCTCTCCTAA+AGG | + | chr4.4:10071081-10071100 | None:intergenic | 35.0% | |
| AGAAGGAAAACGGTTATGAA+CGG | + | chr4.4:10070800-10070819 | None:intergenic | 35.0% | |
| ATTGGTAGAAAATATACCGG+GGG | + | chr4.4:10071690-10071709 | None:intergenic | 35.0% | |
| CATTGGTAGAAAATATACCG+GGG | + | chr4.4:10071691-10071710 | None:intergenic | 35.0% | |
| CCAATACCAATTCCAACTAA+CGG | + | chr4.4:10070901-10070920 | None:intergenic | 35.0% | |
| CGTTAGTTGGAATTGGTATT+GGG | - | chr4.4:10070899-10070918 | MS.gene28113:intron | 35.0% | |
| CTAACATATTTGATGCGGAT+AGG | - | chr4.4:10071446-10071465 | MS.gene28113:intron | 35.0% | |
| TAAGGGTGAATGTGAATTTC+AGG | - | chr4.4:10071349-10071368 | MS.gene28113:intron | 35.0% | |
| ! | ACCATGGATTTTATTAGGCT+GGG | - | chr4.4:10071868-10071887 | MS.gene28113:CDS | 35.0% |
| ! | ATTTCAGGTAATTAGGGGAT+TGG | - | chr4.4:10071364-10071383 | MS.gene28113:intron | 35.0% |
| ! | TGTGATTATGTTGAGGGTTT+TGG | - | chr4.4:10071045-10071064 | MS.gene28113:intron | 35.0% |
| !! | GTGTGATTTGCTACTAAACA+TGG | - | chr4.4:10071609-10071628 | MS.gene28113:CDS | 35.0% |
| AAAAAGCAGCTACAGTGCTA+TGG | - | chr4.4:10071714-10071733 | MS.gene28113:intron | 40.0% | |
| AACGAAAAGAAGCCGTTAGT+TGG | - | chr4.4:10070886-10070905 | MS.gene28113:intron | 40.0% | |
| AAGAAGCCGTTAGTTGGAAT+TGG | - | chr4.4:10070892-10070911 | MS.gene28113:intron | 40.0% | |
| ATAGGTGACTGCAATATACC+GGG | - | chr4.4:10071980-10071999 | MS.gene28113:CDS | 40.0% | |
| ATTCCTCCATTGTTAGCGTA+TGG | - | chr4.4:10071529-10071548 | MS.gene28113:intron | 40.0% | |
| ATTGTAGCAGCATGGGTAAT+GGG | - | chr4.4:10070922-10070941 | MS.gene28113:intron | 40.0% | |
| CCGTTAGTTGGAATTGGTAT+TGG | - | chr4.4:10070898-10070917 | MS.gene28113:intron | 40.0% | |
| GTAATTAGGGGATTGGATCA+GGG | - | chr4.4:10071371-10071390 | MS.gene28113:intron | 40.0% | |
| TATTGGGATTGTAGCAGCAT+GGG | - | chr4.4:10070915-10070934 | MS.gene28113:intron | 40.0% | |
| TCCCAGCCTAATAAAATCCA+TGG | + | chr4.4:10071872-10071891 | None:intergenic | 40.0% | |
| TGCTGAGCATAACACCTTAT+TGG | + | chr4.4:10070857-10070876 | None:intergenic | 40.0% | |
| TTAATACCTATCCGCATTGG+AGG | + | chr4.4:10071419-10071438 | None:intergenic | 40.0% | |
| TTGGTAGAAAATATACCGGG+GGG | + | chr4.4:10071689-10071708 | None:intergenic | 40.0% | |
| ! | ATTGGATCAGGGAATTTTGG+GGG | - | chr4.4:10071382-10071401 | MS.gene28113:intron | 40.0% |
| ! | GACCATGGATTTTATTAGGC+TGG | - | chr4.4:10071867-10071886 | MS.gene28113:CDS | 40.0% |
| ! | GATTGGATCAGGGAATTTTG+GGG | - | chr4.4:10071381-10071400 | MS.gene28113:intron | 40.0% |
| ! | GGATTGGATCAGGGAATTTT+GGG | - | chr4.4:10071380-10071399 | MS.gene28113:intron | 40.0% |
| !! | AGCATGGGTAATGGGATTAA+CGG | - | chr4.4:10070930-10070949 | MS.gene28113:intron | 40.0% |
| !! | TTTGGTGATGAAGCTCCTTT+AGG | - | chr4.4:10071063-10071082 | MS.gene28113:intron | 40.0% |
| ACACCAATACGCATAGTGAG+TGG | + | chr4.4:10071257-10071276 | None:intergenic | 45.0% | |
| CTCTCATTCTCACTCTGTTC+AGG | - | chr4.4:10070769-10070788 | MS.gene28113:CDS | 45.0% | |
| GATTGTAGCAGCATGGGTAA+TGG | - | chr4.4:10070921-10070940 | MS.gene28113:intron | 45.0% | |
| GGTAATTAGGGGATTGGATC+AGG | - | chr4.4:10071370-10071389 | MS.gene28113:intron | 45.0% | |
| GTATTGGGATTGTAGCAGCA+TGG | - | chr4.4:10070914-10070933 | MS.gene28113:intron | 45.0% | |
| GTGTTATGCTCAGCACGTAA+CGG | - | chr4.4:10070862-10070881 | MS.gene28113:intron | 45.0% | |
| TACAGAAGAGTTGCATTGCC+CGG | + | chr4.4:10072001-10072020 | None:intergenic | 45.0% | |
| TATACTGCTAGATTCGCCGA+TGG | - | chr4.4:10071197-10071216 | MS.gene28113:intron | 45.0% | |
| TCAGGACCATACGCTAACAA+TGG | + | chr4.4:10071538-10071557 | None:intergenic | 45.0% | |
| ! | CTCACTATGCGTATTGGTGT+TGG | - | chr4.4:10071257-10071276 | MS.gene28113:CDS | 45.0% |
| ! | GGGATTGGATCAGGGAATTT+TGG | - | chr4.4:10071379-10071398 | MS.gene28113:intron | 45.0% |
| ! | TTGGATCAGGGAATTTTGGG+GGG | - | chr4.4:10071383-10071402 | MS.gene28113:intron | 45.0% |
| !! | TATGCGTATTGGTGTTGGCA+AGG | - | chr4.4:10071262-10071281 | MS.gene28113:CDS | 45.0% |
| AGGCCACTCACTATGCGTAT+TGG | - | chr4.4:10071251-10071270 | MS.gene28113:intron | 50.0% | |
| GGACCATACGCTAACAATGG+AGG | + | chr4.4:10071535-10071554 | None:intergenic | 50.0% | |
| !! | GGTAATGGGATTAACGGCGT+TGG | - | chr4.4:10070936-10070955 | MS.gene28113:intron | 50.0% |
| AAACCTGAGAAGCAGCCTGC+AGG | + | chr4.4:10071562-10071581 | None:intergenic | 55.0% | |
| ! | GGGATTAACGGCGTTGGATG+CGG | - | chr4.4:10070942-10070961 | MS.gene28113:intron | 55.0% |
| ! | GTGAAGGCGTACCTCCAATG+CGG | - | chr4.4:10071405-10071424 | MS.gene28113:CDS | 55.0% |
| !! | CAGGGAATTTTGGGGGGTGA+AGG | - | chr4.4:10071389-10071408 | MS.gene28113:intron | 55.0% |
| GAACCTGCAGGCTGCTTCTC+AGG | - | chr4.4:10071556-10071575 | MS.gene28113:CDS | 60.0% | |
| GAGAAGCAGCCTGCAGGTTC+AGG | + | chr4.4:10071556-10071575 | None:intergenic | 60.0% | |
| GCGTATGGTCCTGAACCTGC+AGG | - | chr4.4:10071544-10071563 | MS.gene28113:intron | 60.0% | |
| GGCGTACCTCCAATGCGGAT+AGG | - | chr4.4:10071410-10071429 | MS.gene28113:CDS | 60.0% | |
| TCCATTGCGCGTACGCCCCC+CGG | - | chr4.4:10071671-10071690 | MS.gene28113:intron | 70.0% | |
| ! | ACCGGGGGGCGTACGCGCAA+TGG | + | chr4.4:10071675-10071694 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 10070763 | 10072066 | 10070763 | ID=MS.gene28113 |
| chr4.4 | mRNA | 10070763 | 10072066 | 10070763 | ID=MS.gene28113.t1;Parent=MS.gene28113 |
| chr4.4 | exon | 10071731 | 10072066 | 10071731 | ID=MS.gene28113.t1.exon1;Parent=MS.gene28113.t1 |
| chr4.4 | CDS | 10071731 | 10072066 | 10071731 | ID=cds.MS.gene28113.t1;Parent=MS.gene28113.t1 |
| chr4.4 | exon | 10071546 | 10071638 | 10071546 | ID=MS.gene28113.t1.exon2;Parent=MS.gene28113.t1 |
| chr4.4 | CDS | 10071546 | 10071638 | 10071546 | ID=cds.MS.gene28113.t1;Parent=MS.gene28113.t1 |
| chr4.4 | exon | 10071398 | 10071458 | 10071398 | ID=MS.gene28113.t1.exon3;Parent=MS.gene28113.t1 |
| chr4.4 | CDS | 10071398 | 10071458 | 10071398 | ID=cds.MS.gene28113.t1;Parent=MS.gene28113.t1 |
| chr4.4 | exon | 10071252 | 10071320 | 10071252 | ID=MS.gene28113.t1.exon4;Parent=MS.gene28113.t1 |
| chr4.4 | CDS | 10071252 | 10071320 | 10071252 | ID=cds.MS.gene28113.t1;Parent=MS.gene28113.t1 |
| chr4.4 | exon | 10070763 | 10070845 | 10070763 | ID=MS.gene28113.t1.exon5;Parent=MS.gene28113.t1 |
| chr4.4 | CDS | 10070763 | 10070845 | 10070763 | ID=cds.MS.gene28113.t1;Parent=MS.gene28113.t1 |
| Gene Sequence |
| Protein sequence |
