AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54377


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54377.t1 MTR_7g006040 98.684 228 3 0 1 228 167 394 1.20e-166 465
MS.gene54377.t1 MTR_8g064720 70.312 192 52 3 1 188 187 377 1.34e-86 262
MS.gene54377.t1 MTR_5g072900 38.824 170 89 5 33 189 219 386 8.90e-24 98.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54377.t1 AT1G18480 61.638 232 79 5 1 228 166 391 1.23e-91 275
MS.gene54377.t1 AT1G07010 36.471 170 95 4 33 190 204 372 4.45e-21 90.9
MS.gene54377.t1 AT1G07010 36.471 170 95 4 33 190 208 376 4.60e-21 90.9
MS.gene54377.t1 AT1G07010 36.471 170 95 4 33 190 219 387 5.97e-21 90.5

Find 70 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGCTAGGGTTGCGGCTTTA+AGG 0.182522 7.4:-93654242 MS.gene54377:CDS
TTGAAAGAACATGTTGATTA+TGG 0.215908 7.4:-93654132 MS.gene54377:CDS
TGAGGTTAGTGATTGGTATA+AGG 0.246230 7.4:-93654091 MS.gene54377:CDS
AAGAGTTTCATGATGGGTTT+AGG 0.248900 7.4:-93654263 MS.gene54377:CDS
TGGTGTTAAGAGGATGATTA+TGG 0.249368 7.4:-93653944 MS.gene54377:CDS
TGGTGGTTTGCCCGAGGTTT+TGG 0.263789 7.4:-93653836 MS.gene54377:CDS
ACGTGGCGTTTCGTGGTGTT+AGG 0.288942 7.4:-93654287 MS.gene54377:CDS
TTATTCATCTGATTATATAA+AGG 0.310568 7.4:+93653775 None:intergenic
TAATGGTGTCTGCGAAAATA+AGG 0.312538 7.4:-93653896 MS.gene54377:CDS
TACACAAAATGTTACTGTTT+TGG 0.322237 7.4:-93654190 MS.gene54377:CDS
TTTATATAATCAGATGAATA+AGG 0.349298 7.4:-93653773 MS.gene54377:CDS
GACAATAAGGCGGCGAAAAC+CGG 0.357356 7.4:+93654055 None:intergenic
GAGGTTAGTGATTGGTATAA+GGG 0.359259 7.4:-93654090 MS.gene54377:CDS
GATTGGTATAAGGGTTTGTT+TGG 0.360244 7.4:-93654081 MS.gene54377:CDS
GGGAAGGTGGAAGAAGGGTT+TGG 0.362537 7.4:-93653736 MS.gene54377:CDS
CGAATGGACCGATTTCAAAA+AGG 0.363226 7.4:-93654218 MS.gene54377:CDS
ATTCAGAAGGAAGGGATTAA+TGG 0.366442 7.4:-93653913 MS.gene54377:CDS
AGAGTTTCATGATGGGTTTA+GGG 0.373309 7.4:-93654262 MS.gene54377:CDS
GCATTCCTTCCCCGACAATA+AGG 0.375199 7.4:+93654042 None:intergenic
TTCATGATGGGTTTAGGGCT+AGG 0.377495 7.4:-93654257 MS.gene54377:CDS
GGGGAAGGAATGCACTTGTT+TGG 0.383176 7.4:-93654032 MS.gene54377:CDS
TATGGGTTGGAGAAGATTAA+TGG 0.389368 7.4:-93654114 MS.gene54377:CDS
TGAAAGAACATGTTGATTAT+GGG 0.411575 7.4:-93654131 MS.gene54377:CDS
ATAAGGGTTTGTTTGGTAAC+CGG 0.411872 7.4:-93654074 MS.gene54377:CDS
TCATGATGGGTTTAGGGCTA+GGG 0.424497 7.4:-93654256 MS.gene54377:CDS
CGACGATTCCTGGTGTTAAG+AGG 0.425093 7.4:-93653954 MS.gene54377:CDS
ATGTAAAGGTTTGGAAGAAA+AGG 0.436989 7.4:-93654334 MS.gene54377:CDS
CGTGGCGTTTCGTGGTGTTA+GGG 0.450367 7.4:-93654286 MS.gene54377:CDS
CGCCGCCTTATTGTCGGGGA+AGG 0.455393 7.4:-93654047 MS.gene54377:CDS
AATGTTCACGTGGCGTTTCG+TGG 0.456234 7.4:-93654294 MS.gene54377:CDS
TCTGCGAAAATAAGGCGATT+AGG 0.459167 7.4:-93653888 MS.gene54377:CDS
GATGTTGGGAAGGTGGAAGA+AGG 0.466879 7.4:-93653742 MS.gene54377:CDS
AATAAGGAAAATGTGGATGT+TGG 0.471510 7.4:-93653757 MS.gene54377:CDS
TGGGTTTAGGGCTAGGGTTG+CGG 0.472943 7.4:-93654250 MS.gene54377:CDS
GGTGTTAAGAGGATGATTAT+GGG 0.476200 7.4:-93653943 MS.gene54377:CDS
AAATGTGGATGTTGGGAAGG+TGG 0.504316 7.4:-93653749 MS.gene54377:CDS
TCAGATGAATAAGGAAAATG+TGG 0.505988 7.4:-93653764 MS.gene54377:CDS
GTTAGGGAAGAGTTTCATGA+TGG 0.506126 7.4:-93654270 MS.gene54377:CDS
ATGTTGGGAAGGTGGAAGAA+GGG 0.512967 7.4:-93653741 MS.gene54377:CDS
AGATTGACCGTTATGGGGTG+AGG 0.524683 7.4:-93653813 MS.gene54377:CDS
GCCTTGACTTCCACTTGCCT+AGG 0.528320 7.4:+93653685 None:intergenic
GCAAGTGGAAGTCAAGGCTT+AGG 0.533119 7.4:-93653680 None:intergenic
ATGTCGAAAGGATGCGGTGG+TGG 0.533822 7.4:-93653853 MS.gene54377:CDS
CGGTCAATCTCCAAAACCTC+GGG 0.534492 7.4:+93653826 None:intergenic
TTAATGGTGAGGTTAGTGAT+TGG 0.539710 7.4:-93654098 MS.gene54377:CDS
ATAAGGAAAATGTGGATGTT+GGG 0.548289 7.4:-93653756 MS.gene54377:CDS
ACCTAGGCAAGTGGAAGTCA+AGG 0.549753 7.4:-93653686 MS.gene54377:CDS
TTAGGGAAGAGTTTCATGAT+GGG 0.558605 7.4:-93654269 MS.gene54377:CDS
ATGCGGTGGTGGTTTGCCCG+AGG 0.563566 7.4:-93653842 MS.gene54377:CDS
TTGTTGCTTAATAATCAAGA+TGG 0.574078 7.4:-93653712 MS.gene54377:CDS
ATCGATGTTGGTATGTCGAA+AGG 0.575221 7.4:-93653865 MS.gene54377:CDS
GTTGGAGAAGATTAATGGTG+AGG 0.579243 7.4:-93654109 MS.gene54377:CDS
GGGCATACGATTCAGAAGGA+AGG 0.584494 7.4:-93653922 MS.gene54377:CDS
TCAATATCCTCACCCCATAA+CGG 0.588832 7.4:+93653806 None:intergenic
ACGGTCAATCTCCAAAACCT+CGG 0.600316 7.4:+93653825 None:intergenic
GGCATACGATTCAGAAGGAA+GGG 0.601131 7.4:-93653921 MS.gene54377:CDS
AGAACATGTTGATTATGGGT+TGG 0.606486 7.4:-93654127 MS.gene54377:CDS
AAGGCGATTAGGATCGATGT+TGG 0.608483 7.4:-93653877 MS.gene54377:CDS
GGAAAATGTGGATGTTGGGA+AGG 0.610345 7.4:-93653752 MS.gene54377:CDS
ATAATCATCCTCTTAACACC+AGG 0.619952 7.4:+93653946 None:intergenic
GTTGCGGCTTTAAGGCCGAA+TGG 0.620496 7.4:-93654234 MS.gene54377:CDS
TTTGGAGATTGACCGTTATG+GGG 0.621026 7.4:-93653818 MS.gene54377:CDS
TATGGGGCATACGATTCAGA+AGG 0.628721 7.4:-93653926 MS.gene54377:CDS
ATAATCAAGATGGTAGACCT+AGG 0.629740 7.4:-93653702 MS.gene54377:CDS
GTGTTAAGAGGATGATTATG+GGG 0.631883 7.4:-93653942 MS.gene54377:CDS
AGATGGTAGACCTAGGCAAG+TGG 0.641426 7.4:-93653695 MS.gene54377:CDS
GTTGGTATGTCGAAAGGATG+CGG 0.642403 7.4:-93653859 MS.gene54377:CDS
TTCCTTCCCCGACAATAAGG+CGG 0.649751 7.4:+93654045 None:intergenic
TCCATTAGAAAATGTTCACG+TGG 0.684935 7.4:-93654304 MS.gene54377:CDS
GGTATGTCGAAAGGATGCGG+TGG 0.743976 7.4:-93653856 MS.gene54377:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTCATCTGATTATATAA+AGG + chr7.4:93654254-93654273 None:intergenic 15.0%
!! TTTATATAATCAGATGAATA+AGG - chr7.4:93654253-93654272 MS.gene54377:CDS 15.0%
! GAAAAATCTATGTAAAGGTT+TGG - chr7.4:93653683-93653702 MS.gene54377:CDS 25.0%
!! TACACAAAATGTTACTGTTT+TGG - chr7.4:93653836-93653855 MS.gene54377:CDS 25.0%
!! TGAAAGAACATGTTGATTAT+GGG - chr7.4:93653895-93653914 MS.gene54377:CDS 25.0%
!! TTGAAAGAACATGTTGATTA+TGG - chr7.4:93653894-93653913 MS.gene54377:CDS 25.0%
!! TTGTTGCTTAATAATCAAGA+TGG - chr7.4:93654314-93654333 MS.gene54377:CDS 25.0%
!!! GTAAAGAACCTTTTTGAAAT+CGG + chr7.4:93653819-93653838 None:intergenic 25.0%
AATAAGGAAAATGTGGATGT+TGG - chr7.4:93654269-93654288 MS.gene54377:CDS 30.0%
ATAAGGAAAATGTGGATGTT+GGG - chr7.4:93654270-93654289 MS.gene54377:CDS 30.0%
ATGTAAAGGTTTGGAAGAAA+AGG - chr7.4:93653692-93653711 MS.gene54377:CDS 30.0%
TCAGATGAATAAGGAAAATG+TGG - chr7.4:93654262-93654281 MS.gene54377:CDS 30.0%
! GATTCAATTTTTGTTCATGG+TGG - chr7.4:93653867-93653886 MS.gene54377:CDS 30.0%
! GGCTTAGAAAATTTTCAGAT+GGG - chr7.4:93654015-93654034 MS.gene54377:CDS 30.0%
! GGTGATTCAATTTTTGTTCA+TGG - chr7.4:93653864-93653883 MS.gene54377:CDS 30.0%
! TGGCTTAGAAAATTTTCAGA+TGG - chr7.4:93654014-93654033 MS.gene54377:CDS 30.0%
!!! AATGTTACTGTTTTGGTAGT+TGG - chr7.4:93653843-93653862 MS.gene54377:CDS 30.0%
ATAATCAAGATGGTAGACCT+AGG - chr7.4:93654324-93654343 MS.gene54377:CDS 35.0%
ATAATCATCCTCTTAACACC+AGG + chr7.4:93654083-93654102 None:intergenic 35.0%
ATTCAGAAGGAAGGGATTAA+TGG - chr7.4:93654113-93654132 MS.gene54377:CDS 35.0%
GAGGTTAGTGATTGGTATAA+GGG - chr7.4:93653936-93653955 MS.gene54377:CDS 35.0%
GGTGTTAAGAGGATGATTAT+GGG - chr7.4:93654083-93654102 MS.gene54377:CDS 35.0%
GTGTTAAGAGGATGATTATG+GGG - chr7.4:93654084-93654103 MS.gene54377:CDS 35.0%
TAATGGTGTCTGCGAAAATA+AGG - chr7.4:93654130-93654149 MS.gene54377:CDS 35.0%
TCCATTAGAAAATGTTCACG+TGG - chr7.4:93653722-93653741 MS.gene54377:CDS 35.0%
TGAGGTTAGTGATTGGTATA+AGG - chr7.4:93653935-93653954 MS.gene54377:CDS 35.0%
TTAATGGTGAGGTTAGTGAT+TGG - chr7.4:93653928-93653947 MS.gene54377:CDS 35.0%
TTAGGGAAGAGTTTCATGAT+GGG - chr7.4:93653757-93653776 MS.gene54377:CDS 35.0%
! AGAACATGTTGATTATGGGT+TGG - chr7.4:93653899-93653918 MS.gene54377:CDS 35.0%
! ATAAGGGTTTGTTTGGTAAC+CGG - chr7.4:93653952-93653971 MS.gene54377:CDS 35.0%
! GATTGGTATAAGGGTTTGTT+TGG - chr7.4:93653945-93653964 MS.gene54377:CDS 35.0%
! TGGTGTTAAGAGGATGATTA+TGG - chr7.4:93654082-93654101 MS.gene54377:CDS 35.0%
!! AAGAGTTTCATGATGGGTTT+AGG - chr7.4:93653763-93653782 MS.gene54377:CDS 35.0%
!! AGAGTTTCATGATGGGTTTA+GGG - chr7.4:93653764-93653783 MS.gene54377:CDS 35.0%
!! TATGGGTTGGAGAAGATTAA+TGG - chr7.4:93653912-93653931 MS.gene54377:CDS 35.0%
!! TTTTGGAGATTGACCGTTAT+GGG - chr7.4:93654207-93654226 MS.gene54377:CDS 35.0%
!!! CTTTTTGAAATCGGTCCATT+CGG + chr7.4:93653810-93653829 None:intergenic 35.0%
ATCGATGTTGGTATGTCGAA+AGG - chr7.4:93654161-93654180 MS.gene54377:CDS 40.0%
GTTAGGGAAGAGTTTCATGA+TGG - chr7.4:93653756-93653775 MS.gene54377:CDS 40.0%
GTTGGAGAAGATTAATGGTG+AGG - chr7.4:93653917-93653936 MS.gene54377:CDS 40.0%
TCAATATCCTCACCCCATAA+CGG + chr7.4:93654223-93654242 None:intergenic 40.0%
TCTGCGAAAATAAGGCGATT+AGG - chr7.4:93654138-93654157 MS.gene54377:CDS 40.0%
TTTGGAGATTGACCGTTATG+GGG - chr7.4:93654208-93654227 MS.gene54377:CDS 40.0%
! CGAATGGACCGATTTCAAAA+AGG - chr7.4:93653808-93653827 MS.gene54377:CDS 40.0%
! GCCACGTGAACATTTTCTAA+TGG + chr7.4:93653726-93653745 None:intergenic 40.0%
!!! GTTTTGGAGATTGACCGTTA+TGG - chr7.4:93654206-93654225 MS.gene54377:CDS 40.0%
AAATGTGGATGTTGGGAAGG+TGG - chr7.4:93654277-93654296 MS.gene54377:CDS 45.0%
AAGGCGATTAGGATCGATGT+TGG - chr7.4:93654149-93654168 MS.gene54377:CDS 45.0%
ACGGTCAATCTCCAAAACCT+CGG + chr7.4:93654204-93654223 None:intergenic 45.0%
ATGTTGGGAAGGTGGAAGAA+GGG - chr7.4:93654285-93654304 MS.gene54377:CDS 45.0%
GGAAAATGTGGATGTTGGGA+AGG - chr7.4:93654274-93654293 MS.gene54377:CDS 45.0%
GGCATACGATTCAGAAGGAA+GGG - chr7.4:93654105-93654124 MS.gene54377:CDS 45.0%
GTTGGTATGTCGAAAGGATG+CGG - chr7.4:93654167-93654186 MS.gene54377:CDS 45.0%
TATGGGGCATACGATTCAGA+AGG - chr7.4:93654100-93654119 MS.gene54377:CDS 45.0%
! CATGTTTTGTCGACGATTCC+TGG - chr7.4:93654062-93654081 MS.gene54377:CDS 45.0%
!! TCATGATGGGTTTAGGGCTA+GGG - chr7.4:93653770-93653789 MS.gene54377:CDS 45.0%
!! TTCATGATGGGTTTAGGGCT+AGG - chr7.4:93653769-93653788 MS.gene54377:CDS 45.0%
AATGTTCACGTGGCGTTTCG+TGG - chr7.4:93653732-93653751 MS.gene54377:CDS 50.0%
ACCTAGGCAAGTGGAAGTCA+AGG - chr7.4:93654340-93654359 MS.gene54377:CDS 50.0%
AGATGGTAGACCTAGGCAAG+TGG - chr7.4:93654331-93654350 MS.gene54377:CDS 50.0%
AGATTGACCGTTATGGGGTG+AGG - chr7.4:93654213-93654232 MS.gene54377:CDS 50.0%
CGGTCAATCTCCAAAACCTC+GGG + chr7.4:93654203-93654222 None:intergenic 50.0%
GACAATAAGGCGGCGAAAAC+CGG + chr7.4:93653974-93653993 None:intergenic 50.0%
GATGTTGGGAAGGTGGAAGA+AGG - chr7.4:93654284-93654303 MS.gene54377:CDS 50.0%
GCATTCCTTCCCCGACAATA+AGG + chr7.4:93653987-93654006 None:intergenic 50.0%
GGGCATACGATTCAGAAGGA+AGG - chr7.4:93654104-93654123 MS.gene54377:CDS 50.0%
GGGGAAGGAATGCACTTGTT+TGG - chr7.4:93653994-93654013 MS.gene54377:CDS 50.0%
TTCCTTCCCCGACAATAAGG+CGG + chr7.4:93653984-93654003 None:intergenic 50.0%
! CGACGATTCCTGGTGTTAAG+AGG - chr7.4:93654072-93654091 MS.gene54377:CDS 50.0%
!! GGTTTTCGCCGCCTTATTGT+CGG - chr7.4:93653973-93653992 MS.gene54377:CDS 50.0%
!! GTTTTCGCCGCCTTATTGTC+GGG - chr7.4:93653974-93653993 MS.gene54377:CDS 50.0%
!! TTTTCGCCGCCTTATTGTCG+GGG - chr7.4:93653975-93653994 MS.gene54377:CDS 50.0%
ACGTGGCGTTTCGTGGTGTT+AGG - chr7.4:93653739-93653758 MS.gene54377:CDS 55.0%
ATGTCGAAAGGATGCGGTGG+TGG - chr7.4:93654173-93654192 MS.gene54377:CDS 55.0%
GCCTTGACTTCCACTTGCCT+AGG + chr7.4:93654344-93654363 None:intergenic 55.0%
GGGAAGGTGGAAGAAGGGTT+TGG - chr7.4:93654290-93654309 MS.gene54377:CDS 55.0%
GGTATGTCGAAAGGATGCGG+TGG - chr7.4:93654170-93654189 MS.gene54377:CDS 55.0%
! CGTGGCGTTTCGTGGTGTTA+GGG - chr7.4:93653740-93653759 MS.gene54377:CDS 55.0%
! TGGGTTTAGGGCTAGGGTTG+CGG - chr7.4:93653776-93653795 MS.gene54377:CDS 55.0%
!! GTTGCGGCTTTAAGGCCGAA+TGG - chr7.4:93653792-93653811 MS.gene54377:CDS 55.0%
!! TGGTGGTTTGCCCGAGGTTT+TGG - chr7.4:93654190-93654209 MS.gene54377:CDS 55.0%
GGGCTAGGGTTGCGGCTTTA+AGG - chr7.4:93653784-93653803 MS.gene54377:CDS 60.0%
! ATGCGGTGGTGGTTTGCCCG+AGG - chr7.4:93654184-93654203 MS.gene54377:CDS 65.0%
! CGCCGCCTTATTGTCGGGGA+AGG - chr7.4:93653979-93653998 MS.gene54377:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 93653681 93654367 93653681 ID=MS.gene54377
chr7.4 mRNA 93653681 93654367 93653681 ID=MS.gene54377.t1;Parent=MS.gene54377
chr7.4 exon 93653681 93654367 93653681 ID=MS.gene54377.t1.exon1;Parent=MS.gene54377.t1
chr7.4 CDS 93653681 93654367 93653681 ID=cds.MS.gene54377.t1;Parent=MS.gene54377.t1
Gene Sequence

>MS.gene54377

ATGAAAAATCTATGTAAAGGTTTGGAAGAAAAGGTTGTAGATCCATTAGAAAATGTTCACGTGGCGTTTCGTGGTGTTAGGGAAGAGTTTCATGATGGGTTTAGGGCTAGGGTTGCGGCTTTAAGGCCGAATGGACCGATTTCAAAAAGGTTCTTTACACAAAATGTTACTGTTTTGGTAGTTGGTGATTCAATTTTTGTTCATGGTGGTTTGTTGAAAGAACATGTTGATTATGGGTTGGAGAAGATTAATGGTGAGGTTAGTGATTGGTATAAGGGTTTGTTTGGTAACCGGTTTTCGCCGCCTTATTGTCGGGGAAGGAATGCACTTGTTTGGCTTAGAAAATTTTCAGATGGGAGTTGTGATTGTTCGAGTCTTGAGCATGTTTTGTCGACGATTCCTGGTGTTAAGAGGATGATTATGGGGCATACGATTCAGAAGGAAGGGATTAATGGTGTCTGCGAAAATAAGGCGATTAGGATCGATGTTGGTATGTCGAAAGGATGCGGTGGTGGTTTGCCCGAGGTTTTGGAGATTGACCGTTATGGGGTGAGGATATTGACATCAAATCCTTTATATAATCAGATGAATAAGGAAAATGTGGATGTTGGGAAGGTGGAAGAAGGGTTTGGATTGTTGCTTAATAATCAAGATGGTAGACCTAGGCAAGTGGAAGTCAAGGCTTAG

Protein sequence

>MS.gene54377.t1

MKNLCKGLEEKVVDPLENVHVAFRGVREEFHDGFRARVAALRPNGPISKRFFTQNVTVLVVGDSIFVHGGLLKEHVDYGLEKINGEVSDWYKGLFGNRFSPPYCRGRNALVWLRKFSDGSCDCSSLEHVLSTIPGVKRMIMGHTIQKEGINGVCENKAIRIDVGMSKGCGGGLPEVLEIDRYGVRILTSNPLYNQMNKENVDVGKVEEGFGLLLNNQDGRPRQVEVKA