Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87473.t1 | XP_013446388.1 | 95.5 | 220 | 10 | 0 | 25 | 244 | 1 | 220 | 3.00E-112 | 414.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87473.t1 | F4J220 | 51.6 | 157 | 74 | 2 | 91 | 245 | 60 | 216 | 6.3e-34 | 146.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87473.t1 | A0A072U3M9 | 95.5 | 220 | 10 | 0 | 25 | 244 | 1 | 220 | 2.2e-112 | 414.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene87473 | 0.853501 | 2.29E-61 | -1.69E-46 |
| MS.gene049222 | MS.gene87473 | 0.834185 | 3.47E-56 | -1.69E-46 |
| MS.gene049232 | MS.gene87473 | 0.840118 | 1.05E-57 | -1.69E-46 |
| MS.gene049285 | MS.gene87473 | 0.835159 | 1.97E-56 | -1.69E-46 |
| MS.gene049320 | MS.gene87473 | 0.835061 | 2.09E-56 | -1.69E-46 |
| MS.gene049609 | MS.gene87473 | 0.817769 | 2.78E-52 | -1.69E-46 |
| MS.gene049627 | MS.gene87473 | 0.801265 | 9.77E-49 | -1.69E-46 |
| MS.gene049633 | MS.gene87473 | 0.809924 | 1.49E-50 | -1.69E-46 |
| MS.gene049657 | MS.gene87473 | 0.827691 | 1.36E-54 | -1.69E-46 |
| MS.gene049865 | MS.gene87473 | 0.844376 | 7.85E-59 | -1.69E-46 |
| MS.gene05010 | MS.gene87473 | 0.850001 | 2.25E-60 | -1.69E-46 |
| MS.gene050170 | MS.gene87473 | 0.897583 | 1.22E-76 | -1.69E-46 |
| MS.gene050323 | MS.gene87473 | 0.853942 | 1.71E-61 | -1.69E-46 |
| MS.gene050496 | MS.gene87473 | 0.869035 | 4.19E-66 | -1.69E-46 |
| MS.gene050504 | MS.gene87473 | 0.864242 | 1.40E-64 | -1.69E-46 |
| MS.gene050518 | MS.gene87473 | 0.832574 | 8.75E-56 | -1.69E-46 |
| MS.gene050577 | MS.gene87473 | 0.890012 | 1.45E-73 | -1.69E-46 |
| MS.gene050612 | MS.gene87473 | 0.832219 | 1.07E-55 | -1.69E-46 |
| MS.gene050675 | MS.gene87473 | 0.823625 | 1.25E-53 | -1.69E-46 |
| MS.gene050699 | MS.gene87473 | 0.856037 | 4.22E-62 | -1.69E-46 |
| MS.gene050747 | MS.gene87473 | 0.826104 | 3.26E-54 | -1.69E-46 |
| MS.gene050772 | MS.gene87473 | 0.823922 | 1.07E-53 | -1.69E-46 |
| MS.gene050868 | MS.gene87473 | 0.844293 | 8.27E-59 | -1.69E-46 |
| MS.gene050871 | MS.gene87473 | 0.843182 | 1.64E-58 | -1.69E-46 |
| MS.gene050876 | MS.gene87473 | 0.810109 | 1.36E-50 | -1.69E-46 |
| MS.gene050883 | MS.gene87473 | -0.814459 | 1.52E-51 | -1.69E-46 |
| MS.gene050904 | MS.gene87473 | 0.848314 | 6.63E-60 | -1.69E-46 |
| MS.gene050950 | MS.gene87473 | 0.820632 | 6.19E-53 | -1.69E-46 |
| MS.gene050986 | MS.gene87473 | 0.840651 | 7.65E-58 | -1.69E-46 |
| MS.gene051317 | MS.gene87473 | 0.826414 | 2.75E-54 | -1.69E-46 |
| MS.gene051551 | MS.gene87473 | 0.823825 | 1.12E-53 | -1.69E-46 |
| MS.gene051560 | MS.gene87473 | 0.805476 | 1.31E-49 | -1.69E-46 |
| MS.gene051941 | MS.gene87473 | 0.800918 | 1.15E-48 | -1.69E-46 |
| MS.gene052066 | MS.gene87473 | 0.811576 | 6.53E-51 | -1.69E-46 |
| MS.gene052164 | MS.gene87473 | 0.87084 | 1.08E-66 | -1.69E-46 |
| MS.gene052482 | MS.gene87473 | 0.89571 | 7.40E-76 | -1.69E-46 |
| MS.gene052486 | MS.gene87473 | 0.900224 | 9.07E-78 | -1.69E-46 |
| MS.gene052503 | MS.gene87473 | 0.846386 | 2.24E-59 | -1.69E-46 |
| MS.gene052504 | MS.gene87473 | 0.808145 | 3.58E-50 | -1.69E-46 |
| MS.gene052506 | MS.gene87473 | 0.824163 | 9.37E-54 | -1.69E-46 |
| MS.gene052822 | MS.gene87473 | 0.820355 | 7.17E-53 | -1.69E-46 |
| MS.gene052823 | MS.gene87473 | 0.849256 | 3.63E-60 | -1.69E-46 |
| MS.gene052825 | MS.gene87473 | 0.846702 | 1.84E-59 | -1.69E-46 |
| MS.gene052966 | MS.gene87473 | 0.817325 | 3.50E-52 | -1.69E-46 |
| MS.gene053083 | MS.gene87473 | -0.842876 | 1.98E-58 | -1.69E-46 |
| MS.gene053203 | MS.gene87473 | 0.877165 | 7.82E-69 | -1.69E-46 |
| MS.gene053213 | MS.gene87473 | 0.832098 | 1.15E-55 | -1.69E-46 |
| MS.gene053286 | MS.gene87473 | 0.838613 | 2.59E-57 | -1.69E-46 |
| MS.gene053420 | MS.gene87473 | -0.80261 | 5.17E-49 | -1.69E-46 |
| MS.gene053436 | MS.gene87473 | 0.841994 | 3.39E-58 | -1.69E-46 |
| MS.gene053480 | MS.gene87473 | 0.837369 | 5.41E-57 | -1.69E-46 |
| MS.gene053549 | MS.gene87473 | -0.819451 | 1.15E-52 | -1.69E-46 |
| MS.gene053574 | MS.gene87473 | 0.849424 | 3.26E-60 | -1.69E-46 |
| MS.gene053626 | MS.gene87473 | 0.842943 | 1.90E-58 | -1.69E-46 |
| MS.gene053824 | MS.gene87473 | 0.831507 | 1.61E-55 | -1.69E-46 |
| MS.gene054595 | MS.gene87473 | 0.820076 | 8.30E-53 | -1.69E-46 |
| MS.gene054600 | MS.gene87473 | 0.802476 | 5.51E-49 | -1.69E-46 |
| MS.gene05470 | MS.gene87473 | 0.878301 | 3.14E-69 | -1.69E-46 |
| MS.gene054927 | MS.gene87473 | 0.802863 | 4.58E-49 | -1.69E-46 |
| MS.gene054954 | MS.gene87473 | 0.862004 | 6.89E-64 | -1.69E-46 |
| MS.gene055362 | MS.gene87473 | 0.852251 | 5.22E-61 | -1.69E-46 |
| MS.gene055502 | MS.gene87473 | 0.844523 | 7.17E-59 | -1.69E-46 |
| MS.gene055577 | MS.gene87473 | 0.848938 | 4.45E-60 | -1.69E-46 |
| MS.gene055748 | MS.gene87473 | 0.820694 | 5.99E-53 | -1.69E-46 |
| MS.gene055750 | MS.gene87473 | 0.838225 | 3.26E-57 | -1.69E-46 |
| MS.gene055751 | MS.gene87473 | 0.82785 | 1.25E-54 | -1.69E-46 |
| MS.gene05589 | MS.gene87473 | 0.865337 | 6.36E-65 | -1.69E-46 |
| MS.gene056040 | MS.gene87473 | 0.827136 | 1.85E-54 | -1.69E-46 |
| MS.gene056046 | MS.gene87473 | 0.815419 | 9.34E-52 | -1.69E-46 |
| MS.gene056310 | MS.gene87473 | -0.800013 | 1.76E-48 | -1.69E-46 |
| MS.gene056311 | MS.gene87473 | -0.816505 | 5.34E-52 | -1.69E-46 |
| MS.gene056409 | MS.gene87473 | 0.842141 | 3.10E-58 | -1.69E-46 |
| MS.gene056450 | MS.gene87473 | 0.838426 | 2.90E-57 | -1.69E-46 |
| MS.gene056504 | MS.gene87473 | 0.885051 | 1.13E-71 | -1.69E-46 |
| MS.gene056510 | MS.gene87473 | 0.835602 | 1.53E-56 | -1.69E-46 |
| MS.gene056610 | MS.gene87473 | 0.856557 | 2.97E-62 | -1.69E-46 |
| MS.gene056657 | MS.gene87473 | 0.823623 | 1.25E-53 | -1.69E-46 |
| MS.gene056692 | MS.gene87473 | 0.849282 | 3.57E-60 | -1.69E-46 |
| MS.gene056878 | MS.gene87473 | 0.843818 | 1.11E-58 | -1.69E-46 |
| MS.gene05726 | MS.gene87473 | -0.819848 | 9.36E-53 | -1.69E-46 |
| MS.gene057658 | MS.gene87473 | 0.81744 | 3.29E-52 | -1.69E-46 |
| MS.gene057831 | MS.gene87473 | 0.840557 | 8.10E-58 | -1.69E-46 |
| MS.gene05787 | MS.gene87473 | 0.842953 | 1.89E-58 | -1.69E-46 |
| MS.gene058157 | MS.gene87473 | 0.828224 | 1.01E-54 | -1.69E-46 |
| MS.gene058334 | MS.gene87473 | -0.81961 | 1.06E-52 | -1.69E-46 |
| MS.gene05837 | MS.gene87473 | 0.847434 | 1.16E-59 | -1.69E-46 |
| MS.gene058419 | MS.gene87473 | 0.841147 | 5.67E-58 | -1.69E-46 |
| MS.gene058544 | MS.gene87473 | 0.835114 | 2.03E-56 | -1.69E-46 |
| MS.gene058773 | MS.gene87473 | 0.818395 | 2.00E-52 | -1.69E-46 |
| MS.gene058838 | MS.gene87473 | 0.831568 | 1.55E-55 | -1.69E-46 |
| MS.gene059022 | MS.gene87473 | 0.853256 | 2.70E-61 | -1.69E-46 |
| MS.gene059023 | MS.gene87473 | 0.864131 | 1.52E-64 | -1.69E-46 |
| MS.gene059103 | MS.gene87473 | -0.810792 | 9.67E-51 | -1.69E-46 |
| MS.gene059480 | MS.gene87473 | -0.81137 | 7.24E-51 | -1.69E-46 |
| MS.gene059667 | MS.gene87473 | 0.829128 | 6.12E-55 | -1.69E-46 |
| MS.gene059677 | MS.gene87473 | 0.841492 | 4.60E-58 | -1.69E-46 |
| MS.gene059718 | MS.gene87473 | 0.842407 | 2.64E-58 | -1.69E-46 |
| MS.gene059756 | MS.gene87473 | 0.852298 | 5.06E-61 | -1.69E-46 |
| MS.gene059801 | MS.gene87473 | 0.813622 | 2.33E-51 | -1.69E-46 |
| MS.gene059837 | MS.gene87473 | 0.899668 | 1.58E-77 | -1.69E-46 |
| MS.gene059865 | MS.gene87473 | 0.849944 | 2.34E-60 | -1.69E-46 |
| MS.gene05995 | MS.gene87473 | 0.806699 | 7.25E-50 | -1.69E-46 |
| MS.gene05996 | MS.gene87473 | 0.819842 | 9.39E-53 | -1.69E-46 |
| MS.gene06000 | MS.gene87473 | 0.870357 | 1.55E-66 | -1.69E-46 |
| MS.gene060187 | MS.gene87473 | -0.809874 | 1.52E-50 | -1.69E-46 |
| MS.gene060595 | MS.gene87473 | -0.867753 | 1.08E-65 | -1.69E-46 |
| MS.gene060703 | MS.gene87473 | 0.819502 | 1.12E-52 | -1.69E-46 |
| MS.gene060762 | MS.gene87473 | 0.830084 | 3.58E-55 | -1.69E-46 |
| MS.gene060778 | MS.gene87473 | 0.862869 | 3.73E-64 | -1.69E-46 |
| MS.gene06077 | MS.gene87473 | 0.903243 | 4.24E-79 | -1.69E-46 |
| MS.gene06092 | MS.gene87473 | 0.800588 | 1.34E-48 | -1.69E-46 |
| MS.gene060982 | MS.gene87473 | 0.878127 | 3.61E-69 | -1.69E-46 |
| MS.gene061044 | MS.gene87473 | -0.801489 | 8.79E-49 | -1.69E-46 |
| MS.gene061150 | MS.gene87473 | 0.807338 | 5.31E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene87473 | MS.gene012014 | PPI |
| MS.gene012014 | MS.gene87473 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87473.t1 | MTR_8g077115 | 95.455 | 220 | 10 | 0 | 25 | 244 | 1 | 220 | 1.50e-148 | 417 |
| MS.gene87473.t1 | MTR_8g077120 | 74.299 | 214 | 53 | 1 | 31 | 244 | 1 | 212 | 2.70e-113 | 326 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87473.t1 | AT5G66450 | 53.012 | 166 | 70 | 4 | 86 | 245 | 1 | 164 | 2.21e-47 | 158 |
| MS.gene87473.t1 | AT5G66450 | 52.381 | 168 | 73 | 3 | 83 | 245 | 58 | 223 | 5.81e-47 | 157 |
| MS.gene87473.t1 | AT5G66450 | 52.381 | 168 | 73 | 3 | 83 | 245 | 58 | 223 | 1.31e-46 | 157 |
| MS.gene87473.t1 | AT5G66450 | 52.381 | 168 | 73 | 3 | 83 | 245 | 58 | 223 | 1.31e-46 | 157 |
| MS.gene87473.t1 | AT5G66450 | 52.381 | 168 | 73 | 3 | 83 | 245 | 58 | 223 | 1.88e-46 | 158 |
| MS.gene87473.t1 | AT3G50920 | 51.592 | 157 | 74 | 2 | 91 | 245 | 9 | 165 | 2.62e-46 | 155 |
| MS.gene87473.t1 | AT3G50920 | 51.592 | 157 | 74 | 2 | 91 | 245 | 60 | 216 | 1.50e-45 | 154 |
Find 47 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTTGTTCCTTTCAATCTC+TGG | 0.253264 | 8.3:-26987839 | MS.gene87473:CDS |
| AGTTGTTTGGCTTTGGCATT+TGG | 0.288675 | 8.3:-26985350 | MS.gene87473:CDS |
| ACTCAAACAAATACTTAATC+AGG | 0.308287 | 8.3:-26986359 | MS.gene87473:CDS |
| TACCAGCAGTTGTTTGGCTT+TGG | 0.320403 | 8.3:-26985357 | MS.gene87473:CDS |
| CTTCAAGATCATTCACTTGT+GGG | 0.321620 | 8.3:-26987860 | MS.gene87473:CDS |
| GCAGAAGCCTTATGGTTTGC+AGG | 0.334010 | 8.3:-26986415 | MS.gene87473:CDS |
| CACCATTACCAGCAGTTGTT+TGG | 0.352388 | 8.3:-26985363 | MS.gene87473:CDS |
| TTCTCAATCCATCTTCTTTG+CGG | 0.362994 | 8.3:-26986290 | MS.gene87473:CDS |
| AACAAGAAGCTTTCGTTGAT+AGG | 0.397043 | 8.3:-26987740 | MS.gene87473:CDS |
| GTTTGTTCCTTTCAATCTCT+GGG | 0.399249 | 8.3:-26987838 | MS.gene87473:CDS |
| GGCATGATGCAGAAGCCTTA+TGG | 0.399429 | 8.3:-26986423 | MS.gene87473:CDS |
| CATCCCAGGGTCAGATTTCA+CGG | 0.439728 | 8.3:+26986324 | None:intergenic |
| GAATGTGAAGATGGCATCCC+AGG | 0.440676 | 8.3:+26986310 | None:intergenic |
| GGGTCAGATTTCACGGTTGA+AGG | 0.444202 | 8.3:+26986331 | None:intergenic |
| ACATAGTCTGGCTTTGACAT+TGG | 0.452393 | 8.3:-26983286 | MS.gene87473:intron |
| TGGATAGTAAGTGCCCTCTT+TGG | 0.458814 | 8.3:-26986463 | MS.gene87473:CDS |
| TGCATTACACCTCCGTTTGG+AGG | 0.463599 | 8.3:-26983254 | MS.gene87473:CDS |
| AAACATGACCGCAAAGAAGA+TGG | 0.468037 | 8.3:+26986282 | None:intergenic |
| TTTAGTTCATCTTGGTTGAA+GGG | 0.472928 | 8.3:+26995600 | None:intergenic |
| AGCCAAACAACTGCTGGTAA+TGG | 0.486744 | 8.3:+26985361 | None:intergenic |
| ACTTCAGTCAATATGTCAAA+AGG | 0.494515 | 8.3:-26995636 | None:intergenic |
| GCTTCAAGATCATTCACTTG+TGG | 0.496350 | 8.3:-26987861 | MS.gene87473:CDS |
| TGCCAAAGCCAAACAACTGC+TGG | 0.514847 | 8.3:+26985355 | None:intergenic |
| AAACGGAGGTGTAATGCAAA+AGG | 0.517130 | 8.3:+26983259 | None:intergenic |
| TGTTAGTACGCTCCTCCAAA+CGG | 0.525748 | 8.3:+26983242 | None:intergenic |
| TAAGAGTTGATTCCACGTCC+TGG | 0.534079 | 8.3:+26987688 | None:intergenic |
| GTAAGAACTGCTCAGAGAGA+TGG | 0.542961 | 8.3:-26987792 | MS.gene87473:CDS |
| CAGAAGCCTTATGGTTTGCA+GGG | 0.543056 | 8.3:-26986414 | MS.gene87473:CDS |
| GATCTTGAAGCAGATAAAGT+AGG | 0.543204 | 8.3:+26987873 | None:intergenic |
| GAAGCCTTATGGTTTGCAGG+GGG | 0.547181 | 8.3:-26986412 | MS.gene87473:CDS |
| GCTGTTGTTTACAGAGCAAG+TGG | 0.563321 | 8.3:-26986483 | MS.gene87473:intron |
| CAGAGAGATGGAAAGAGTGA+TGG | 0.564058 | 8.3:-26987780 | MS.gene87473:CDS |
| ATAGCAAAGCGTGGCTGTTG+CGG | 0.565629 | 8.3:+26987965 | None:intergenic |
| CAACCGTGAAATCTGACCCT+GGG | 0.567329 | 8.3:-26986327 | MS.gene87473:CDS |
| AAGCAAGGAATGTCAACAGT+TGG | 0.569697 | 8.3:-26983218 | MS.gene87473:CDS |
| GGAATCAACTCTTAATCGAC+TGG | 0.570438 | 8.3:-26987679 | MS.gene87473:intron |
| ATGGATTGAGAATGTGAAGA+TGG | 0.572584 | 8.3:+26986301 | None:intergenic |
| TCAACCGTGAAATCTGACCC+TGG | 0.578334 | 8.3:-26986328 | MS.gene87473:CDS |
| AAGCCTTATGGTTTGCAGGG+GGG | 0.591130 | 8.3:-26986411 | MS.gene87473:CDS |
| GCGTGGCTGTTGCGGTCATG+TGG | 0.598281 | 8.3:+26987973 | None:intergenic |
| TGGGTGAAAAGTGCAATGGA+TGG | 0.631891 | 8.3:-26987819 | MS.gene87473:CDS |
| AATGTGAAGATGGCATCCCA+GGG | 0.640434 | 8.3:+26986311 | None:intergenic |
| AGAAGCCTTATGGTTTGCAG+GGG | 0.644085 | 8.3:-26986413 | MS.gene87473:CDS |
| GGAGCGTACTAACAGAAGCA+AGG | 0.661458 | 8.3:-26983233 | MS.gene87473:CDS |
| TCTCTGGGTGAAAAGTGCAA+TGG | 0.673354 | 8.3:-26987823 | MS.gene87473:CDS |
| AGATGGATTATAGCAAAGCG+TGG | 0.685105 | 8.3:+26987956 | None:intergenic |
| TAGTACGCTCCTCCAAACGG+AGG | 0.738242 | 8.3:+26983245 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 26983213 | 26995646 | 26983213 | ID=MS.gene87473 |
| chr8.3 | mRNA | 26983213 | 26995646 | 26983213 | ID=MS.gene87473.t1;Parent=MS.gene87473 |
| chr8.3 | exon | 26995596 | 26995646 | 26995596 | ID=MS.gene87473.t1.exon1;Parent=MS.gene87473.t1 |
| chr8.3 | CDS | 26995596 | 26995646 | 26995596 | ID=cds.MS.gene87473.t1;Parent=MS.gene87473.t1 |
| chr8.3 | exon | 26987680 | 26988030 | 26987680 | ID=MS.gene87473.t1.exon2;Parent=MS.gene87473.t1 |
| chr8.3 | CDS | 26987680 | 26988030 | 26987680 | ID=cds.MS.gene87473.t1;Parent=MS.gene87473.t1 |
| chr8.3 | exon | 26986269 | 26986491 | 26986269 | ID=MS.gene87473.t1.exon3;Parent=MS.gene87473.t1 |
| chr8.3 | CDS | 26986269 | 26986491 | 26986269 | ID=cds.MS.gene87473.t1;Parent=MS.gene87473.t1 |
| chr8.3 | exon | 26985340 | 26985413 | 26985340 | ID=MS.gene87473.t1.exon4;Parent=MS.gene87473.t1 |
| chr8.3 | CDS | 26985340 | 26985413 | 26985340 | ID=cds.MS.gene87473.t1;Parent=MS.gene87473.t1 |
| chr8.3 | exon | 26984613 | 26984645 | 26984613 | ID=MS.gene87473.t1.exon5;Parent=MS.gene87473.t1 |
| chr8.3 | CDS | 26984613 | 26984645 | 26984613 | ID=cds.MS.gene87473.t1;Parent=MS.gene87473.t1 |
| chr8.3 | exon | 26983213 | 26983302 | 26983213 | ID=MS.gene87473.t1.exon6;Parent=MS.gene87473.t1 |
| chr8.3 | CDS | 26983213 | 26983302 | 26983213 | ID=cds.MS.gene87473.t1;Parent=MS.gene87473.t1 |
| Gene Sequence |
| Protein sequence |
