Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040889.01.T01 | XP_003625560.1 | 88.315 | 368 | 1 | 1 | 181 | 506 | 10 | 377 | 0 | 655 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048918.01 | MsG0780040889.01 | -0.801226 | 9.949428e-49 | 4.726153e-46 |
| MsG0080049024.01 | MsG0780040889.01 | -0.812901 | 3.356850e-51 | 2.160567e-48 |
| MsG0080049038.01 | MsG0780040889.01 | -0.811277 | 7.588857e-51 | 4.677351e-48 |
| MsG0180000367.01 | MsG0780040889.01 | 0.835195 | 1.933179e-56 | 2.346870e-53 |
| MsG0180000466.01 | MsG0780040889.01 | 0.802932 | 4.437481e-49 | 2.201153e-46 |
| MsG0180000497.01 | MsG0780040889.01 | 0.830684 | 2.555909e-55 | 2.712452e-52 |
| MsG0180001137.01 | MsG0780040889.01 | -0.832395 | 9.689881e-56 | 1.081692e-52 |
| MsG0180001147.01 | MsG0780040889.01 | -0.825633 | 4.213937e-54 | 3.862395e-51 |
| MsG0180001486.01 | MsG0780040889.01 | 0.809196 | 2.132532e-50 | 1.244061e-47 |
| MsG0180001503.01 | MsG0780040889.01 | 0.807776 | 4.285050e-50 | 2.407759e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040889.01.T01 | MTR_7g100500 | 88.315 | 368 | 1 | 1 | 181 | 506 | 10 | 377 | 0.0 | 655 |
| MsG0780040889.01.T01 | MTR_1g067140 | 93.069 | 303 | 19 | 1 | 205 | 505 | 77 | 379 | 0.0 | 583 |
| MsG0780040889.01.T01 | MTR_2g019990 | 84.512 | 297 | 45 | 1 | 204 | 499 | 91 | 387 | 0.0 | 526 |
| MsG0780040889.01.T01 | MTR_1g117060 | 81.818 | 286 | 52 | 0 | 204 | 489 | 99 | 384 | 4.62e-174 | 499 |
| MsG0780040889.01.T01 | MTR_2g095880 | 80.496 | 282 | 55 | 0 | 205 | 486 | 94 | 375 | 1.66e-173 | 495 |
| MsG0780040889.01.T01 | MTR_2g095920 | 70.717 | 321 | 91 | 2 | 176 | 493 | 177 | 497 | 1.20e-166 | 481 |
| MsG0780040889.01.T01 | MTR_4g131900 | 72.664 | 289 | 77 | 1 | 207 | 493 | 106 | 394 | 3.68e-158 | 455 |
| MsG0780040889.01.T01 | MTR_3g050780 | 73.759 | 282 | 74 | 0 | 204 | 485 | 80 | 361 | 3.85e-158 | 457 |
| MsG0780040889.01.T01 | MTR_2g095950 | 66.250 | 320 | 107 | 1 | 168 | 486 | 24 | 343 | 2.40e-156 | 450 |
| MsG0780040889.01.T01 | MTR_2g019990 | 83.267 | 251 | 41 | 1 | 250 | 499 | 1 | 251 | 1.39e-152 | 440 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040889.01.T01 | AT5G02800 | 86.093 | 302 | 40 | 1 | 205 | 504 | 76 | 377 | 0.0 | 538 |
| MsG0780040889.01.T01 | AT5G18610 | 82.353 | 306 | 51 | 2 | 204 | 506 | 85 | 390 | 0.0 | 523 |
| MsG0780040889.01.T01 | AT5G18610 | 82.353 | 306 | 51 | 2 | 204 | 506 | 85 | 390 | 0.0 | 523 |
| MsG0780040889.01.T01 | AT5G18610 | 82.353 | 306 | 51 | 2 | 204 | 506 | 85 | 390 | 0.0 | 523 |
| MsG0780040889.01.T01 | AT5G13160 | 80.000 | 290 | 58 | 0 | 204 | 493 | 88 | 377 | 5.64e-174 | 498 |
| MsG0780040889.01.T01 | AT1G07870 | 76.042 | 288 | 69 | 0 | 205 | 492 | 106 | 393 | 3.20e-166 | 477 |
| MsG0780040889.01.T01 | AT1G07870 | 76.042 | 288 | 69 | 0 | 205 | 492 | 106 | 393 | 1.05e-164 | 477 |
| MsG0780040889.01.T01 | AT2G28590 | 75.089 | 281 | 70 | 0 | 205 | 485 | 101 | 381 | 7.26e-157 | 453 |
| MsG0780040889.01.T01 | AT3G20530 | 73.684 | 285 | 74 | 1 | 204 | 487 | 84 | 368 | 5.28e-156 | 449 |
| MsG0780040889.01.T01 | AT3G24790 | 73.188 | 276 | 74 | 0 | 205 | 480 | 50 | 325 | 2.55e-147 | 426 |
Find 171 sgRNAs with CRISPR-Local
Find 501 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACCAAAATCAGATAACTT+TGG | 0.433869 | 7:+83888729 | None:intergenic |
| AAACGGACTCCAAGGGAACA+GGG | 0.642350 | 7:-83889153 | MsG0780040889.01.T01:CDS |
| AAATATGTGTTTGCTAGGTT+TGG | 0.373619 | 7:-83894815 | None:intergenic |
| AAATCAGATGTTTATAGCTT+TGG | 0.333341 | 7:-83888600 | MsG0780040889.01.T01:CDS |
| AAATCTCGGTATATGACCGG+AGG | 0.652463 | 7:+83888789 | None:intergenic |
| AACACTTGTTAGGGGAGTGT+AGG | 0.577621 | 7:-83894741 | MsG0780040889.01.T01:CDS |
| AACCAAAATCAGATAACTTT+GGG | 0.415584 | 7:+83888730 | None:intergenic |
| AACGTAGAACAAATTCGACC+AGG | 0.653785 | 7:+83887786 | None:intergenic |
| AAGAGGGAGAGGCGTGGAGT+TGG | 0.320128 | 7:-83894789 | MsG0780040889.01.T01:CDS |
| AAGGAGGAGAAGACCTTATA+TGG | 0.398780 | 7:-83894590 | MsG0780040889.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAGGATGAATCTGTGAAT+AGG | - | Chr7:83890800-83890819 | MsG0780040889.01.T01:intron | 30.0% | |
| AAATTAGAGGGAAAAATCAG+AGG | + | Chr7:83890647-83890666 | None:intergenic | 30.0% | |
| AAGAATGAATTTGAAGATCG+CGG | - | Chr7:83890369-83890388 | MsG0780040889.01.T01:intron | 30.0% | |
| AAGATTGCAATATCATGTGT+TGG | - | Chr7:83892956-83892975 | MsG0780040889.01.T01:intron | 30.0% | |
| AATAACCAATAAGGTTGACA+AGG | + | Chr7:83893458-83893477 | None:intergenic | 30.0% | |
| AGACGAACAATGATTTAAAG+GGG | + | Chr7:83893022-83893041 | None:intergenic | 30.0% | |
| AGAGGCTTCGTATTTAATAA+AGG | + | Chr7:83889924-83889943 | None:intergenic | 30.0% | |
| ATTTATTATAGACTAGAGGG+AGG | - | Chr7:83888830-83888849 | MsG0780040889.01.T01:CDS | 30.0% | |
| CAAAATATCATTCGTTATGG+TGG | - | Chr7:83888374-83888393 | MsG0780040889.01.T01:intron | 30.0% | |
| CAAAGCTCAATTATCCAATA+GGG | + | Chr7:83892223-83892242 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 83887728 | 83894833 | 83887728 | ID=MsG0780040889.01;Name=MsG0780040889.01 |
| Chr7 | mRNA | 83887728 | 83894833 | 83887728 | ID=MsG0780040889.01.T01;Parent=MsG0780040889.01;Name=MsG0780040889.01.T01;_AED=0.42;_eAED=0.42;_QI=0|0.5|0.42|0.85|1|1|7|252|506 |
| Chr7 | exon | 83887728 | 83888240 | 83887728 | ID=MsG0780040889.01.T01:exon:11462;Parent=MsG0780040889.01.T01 |
| Chr7 | exon | 83888518 | 83888909 | 83888518 | ID=MsG0780040889.01.T01:exon:11461;Parent=MsG0780040889.01.T01 |
| Chr7 | exon | 83889012 | 83889201 | 83889012 | ID=MsG0780040889.01.T01:exon:11460;Parent=MsG0780040889.01.T01 |
| Chr7 | exon | 83889317 | 83889381 | 83889317 | ID=MsG0780040889.01.T01:exon:11459;Parent=MsG0780040889.01.T01 |
| Chr7 | exon | 83890391 | 83890459 | 83890391 | ID=MsG0780040889.01.T01:exon:11458;Parent=MsG0780040889.01.T01 |
| Chr7 | exon | 83890975 | 83891034 | 83890975 | ID=MsG0780040889.01.T01:exon:11457;Parent=MsG0780040889.01.T01 |
| Chr7 | exon | 83894350 | 83894833 | 83894350 | ID=MsG0780040889.01.T01:exon:11456;Parent=MsG0780040889.01.T01 |
| Chr7 | CDS | 83894350 | 83894833 | 83894350 | ID=MsG0780040889.01.T01:cds;Parent=MsG0780040889.01.T01 |
| Chr7 | CDS | 83890975 | 83891034 | 83890975 | ID=MsG0780040889.01.T01:cds;Parent=MsG0780040889.01.T01 |
| Chr7 | CDS | 83890391 | 83890459 | 83890391 | ID=MsG0780040889.01.T01:cds;Parent=MsG0780040889.01.T01 |
| Chr7 | CDS | 83889317 | 83889381 | 83889317 | ID=MsG0780040889.01.T01:cds;Parent=MsG0780040889.01.T01 |
| Chr7 | CDS | 83889012 | 83889201 | 83889012 | ID=MsG0780040889.01.T01:cds;Parent=MsG0780040889.01.T01 |
| Chr7 | CDS | 83888518 | 83888909 | 83888518 | ID=MsG0780040889.01.T01:cds;Parent=MsG0780040889.01.T01 |
| Chr7 | CDS | 83887980 | 83888240 | 83887980 | ID=MsG0780040889.01.T01:cds;Parent=MsG0780040889.01.T01 |
| Chr7 | three_prime_UTR | 83887728 | 83887979 | 83887728 | ID=MsG0780040889.01.T01:three_prime_utr;Parent=MsG0780040889.01.T01 |
| Gene Sequence |
>MsG0780040889.01.T01 ATGTGTTTGCTAGGTTTGGAGGAAGAGGGAGAGGCGTGGAGTTGGAGGAGAAGACTTTGGGCTTGGGAGGAACACTTGTTAGGGGAGTGTAGGGCTTTACTTGATAATGTTGTTGTGCAGTCTAATGTTTTGACAGGTGGCAATGGGACCAAATCCCCACGAAGGCTATACAGTGAGAGGTGCATATCAGGTGTTGACATCTATGGAAGTACCCATTGTAGAAGGAGGAGAAGACCTTATATGGCACAAACAGGTTCCGTTGAAGGTTTCCATCCTTGCTTGGCGTTTGCTGAAGGACAGGTTACCAACCAGAAACAATCTTCAAAGGCGCGGCATCCTTGCGGCGTCAGACATCACGTGTTTATCAGGCGGCGTATGGAGGAAACCGCCTCCCATATCTTTCTCCATTGTGAAGTTTTTGGTTCTCTATGGCAACACATCAGGTCGTGGATTGGCATTTCTGGAGTTCATCCTCAAAATATTAGTATTTTAATTTTGGGAAATGTTAACCAATGCCCCGGGCACTGGTTAAGGCCTATAAATGGAACTAAGAAGAAGATTGTGAAGATGGAAGTTCAAGATAGTGTTGCTCCTCAGATTAAAGCTACCCCAGAATGTCTTTTGGGGGAGGGAGGCTTTGGTAGAGTATACAAAGGGCATCTGGAAAGTATTAATCAGGTTGTCGCCATCAAGCAACTTGACCGAAACGGACTCCAAGGGAACAGGGAATTCCTTGTTGAAGTATTGATGTTAAGTCTACTTCACCACCCTAACCTTGTCAACCTTATTGGTTATTGCGCCGATGGAGATCAAAGGCTTTTAGTTTATGAATATATGCCATTAGGATGCTTGGAAGATCACTTGCATGATATTTCTCCTGCCAAGAAACGGCTTGACTGGAACACACGAATGAAAATAGCTGCGGGGGCAGCAAAGGGGTTGGAATATCTACACGACAAAGCTAATCCTCCGGTCATATACCGAGATTTGAAGTGCTCCAACATTTTGCTTGGTGAAGGATATCACCCAAAGTTATCTGATTTTGGTTTGGCCAAACTAGGCCCGGTCGGAGAAAACACCCATGTATCGACAAGAGTTATGGGAACCTATGGATATTGTGCTCCAGAGTATGCGATGACTGGTCAACTCACTCTAAAATCAGATGTTTATAGCTTTGGCGTAGTTCTTCTCGAAATAATTACAGGAAGGAAAGCAATTGACTATTCGAAATCCGCAGCAGAACAGAATCTTGTCGCATGGGCTAGACCATTGTTCAAAGATCGAAGGAAATTTTCACAAATGGCCGATCCTATGCTTCAAGGTCAGTATCCTTCAAGGGGGCTATACCAGGCCCTTGCTGTTGCAGCAATGTGTGTTCAGGAACAAGCTAATATGCGTCCGGTTATAGCTGATGTTGTCACGGCTTTGTCTTACCTTGCATTGCAAAAACATGATCCCAATACAGTCCAAAACTCTCGCCTCGCTCCTAGTACTCCTACTAGAACCAGGAGGGGGCGATGA |
| Protein sequence |
>MsG0780040889.01.T01 MCLLGLEEEGEAWSWRRRLWAWEEHLLGECRALLDNVVVQSNVLTGGNGTKSPRRLYSERCISGVDIYGSTHCRRRRRPYMAQTGSVEGFHPCLAFAEGQVTNQKQSSKARHPCGVRHHVFIRRRMEETASHIFLHCEVFGSLWQHIRSWIGISGVHPQNISILILGNVNQCPGHWLRPINGTKKKIVKMEVQDSVAPQIKATPECLLGEGGFGRVYKGHLESINQVVAIKQLDRNGLQGNREFLVEVLMLSLLHHPNLVNLIGYCADGDQRLLVYEYMPLGCLEDHLHDISPAKKRLDWNTRMKIAAGAAKGLEYLHDKANPPVIYRDLKCSNILLGEGYHPKLSDFGLAKLGPVGENTHVSTRVMGTYGYCAPEYAMTGQLTLKSDVYSFGVVLLEIITGRKAIDYSKSAAEQNLVAWARPLFKDRRKFSQMADPMLQGQYPSRGLYQALAVAAMCVQEQANMRPVIADVVTALSYLALQKHDPNTVQNSRLAPSTPTRTRRGR* |
