AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041101.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041101.01.T01 MTR_4g051480 42.308 156 55 5 18 151 680 822 2.86e-25 102
MsG0780041101.01.T01 MTR_4g092300 42.308 156 55 5 18 151 662 804 3.17e-25 102
MsG0780041101.01.T01 MTR_8g085310 42.675 157 57 7 18 151 540 686 4.83e-22 93.6
MsG0780041101.01.T01 MTR_2g017975 95.238 42 2 0 158 199 8 49 2.94e-21 86.7
MsG0780041101.01.T01 MTR_3g053640 35.443 158 69 3 5 151 275 410 2.47e-19 85.5
MsG0780041101.01.T01 MTR_1g094760 40.940 149 53 4 25 151 544 679 6.96e-17 78.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 44 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAAGCGTGGAAAAGTTTA+AGG 0.170460 7:-86605627 MsG0780041101.01.T01:CDS
CTTTGAAATTGTGCAGCTTT+GGG 0.268536 7:+86606148 None:intergenic
TCTTTGAAATTGTGCAGCTT+TGG 0.278538 7:+86606147 None:intergenic
AGCATGGGCTCAAGTTTCTT+TGG 0.334501 7:-86605776 MsG0780041101.01.T01:CDS
ATGTGATGTAGACTCCTTCT+TGG 0.355967 7:+86605819 None:intergenic
ATGATATGCATATATCATTC+AGG 0.382004 7:-86606176 MsG0780041101.01.T01:CDS
GTGTGACCAGACAAGCTTGC+TGG 0.397928 7:-86606120 MsG0780041101.01.T01:CDS
TTATTTCCAGCAAGCTTGTC+TGG 0.399671 7:+86606114 None:intergenic
TCATCATTCATCTACCAATC+TGG 0.425023 7:-86606060 MsG0780041101.01.T01:CDS
AGCTGCACAATTTCAAAGAA+AGG 0.430753 7:-86606143 MsG0780041101.01.T01:CDS
TTTCTTTGGTAAGAGCTGAT+AGG 0.431958 7:-86605762 MsG0780041101.01.T01:CDS
AAAAGTTTAAGGATTCTGAT+TGG 0.433232 7:-86605616 MsG0780041101.01.T01:CDS
CATACGAGCTCATCCCTTCA+AGG 0.437361 7:+86605244 None:intergenic
GACATTTCAGATGATTCCTA+TGG 0.446146 7:-86605649 MsG0780041101.01.T01:CDS
AACGAGGTTCCTAACCTTGA+AGG 0.452298 7:-86605258 MsG0780041101.01.T01:CDS
GAGCTCATCCCTTCAAGGTT+AGG 0.453636 7:+86605249 None:intergenic
TGTTGATATTATTTCCAGAT+TGG 0.454507 7:+86606046 None:intergenic
AAGTGTGTGGCTGCTCTCGA+TGG 0.472530 7:-86605679 MsG0780041101.01.T01:CDS
ATAGGAATCATCTTAGAGAT+AGG 0.479459 7:-86605170 MsG0780041101.01.T01:CDS
CTGATAGGTTAAGAAGGGAT+AGG 0.484536 7:-86605747 MsG0780041101.01.T01:CDS
GTTTAAGGATTCTGATTGGA+GGG 0.496068 7:-86605612 MsG0780041101.01.T01:CDS
ATCATCTTAGAGATAGGATG+TGG 0.498557 7:-86605164 MsG0780041101.01.T01:CDS
GAATGAGGCACTTGCAGCAT+GGG 0.501641 7:-86605791 MsG0780041101.01.T01:CDS
TAAGAGCTGATAGGTTAAGA+AGG 0.504282 7:-86605753 MsG0780041101.01.T01:CDS
TTAACAAGGTTATCTGATGA+AGG 0.516699 7:-86605213 MsG0780041101.01.T01:CDS
GCTGCACAATTTCAAAGAAA+GGG 0.529794 7:-86606142 MsG0780041101.01.T01:CDS
AAGAGCTGATAGGTTAAGAA+GGG 0.537119 7:-86605752 MsG0780041101.01.T01:CDS
CACTCACAGTGAAAAGCGAC+AGG 0.557370 7:-86605854 MsG0780041101.01.T01:CDS
TGATGAAGGTGCAGCTGAGA+TGG 0.559464 7:-86605199 MsG0780041101.01.T01:CDS
AGTTTAAGGATTCTGATTGG+AGG 0.570217 7:-86605613 MsG0780041101.01.T01:CDS
ATCGAGAGCAGCCACACACT+TGG 0.570899 7:+86605681 None:intergenic
CAGCTGAGATGGCAATGGAT+AGG 0.572746 7:-86605188 MsG0780041101.01.T01:CDS
AGTGTGTGGCTGCTCTCGAT+GGG 0.579630 7:-86605678 MsG0780041101.01.T01:CDS
CGTATGTTGAAAACTTAACA+AGG 0.583345 7:-86605227 MsG0780041101.01.T01:CDS
TACATCACATCATATGAATG+AGG 0.591772 7:-86605806 MsG0780041101.01.T01:CDS
TGAATGAGGCACTTGCAGCA+TGG 0.593350 7:-86605792 MsG0780041101.01.T01:CDS
TTGTTCTCTTACCAAGTGTG+TGG 0.604130 7:-86605692 MsG0780041101.01.T01:CDS
ACGAGGTTCCTAACCTTGAA+GGG 0.611095 7:-86605257 MsG0780041101.01.T01:CDS
AAAACTTGAGGCGGTGAACG+AGG 0.615754 7:-86605274 MsG0780041101.01.T01:intron
GGTTCATGTGAGATCCAAGA+AGG 0.621915 7:-86605833 MsG0780041101.01.T01:CDS
ATGATTCCTATGGAAAAGCG+TGG 0.626741 7:-86605639 MsG0780041101.01.T01:CDS
AGGTGCAGCTGAGATGGCAA+TGG 0.647281 7:-86605193 MsG0780041101.01.T01:CDS
AAGAAGGGATAGGAGTGCAG+AGG 0.647414 7:-86605737 MsG0780041101.01.T01:CDS
TCATCTTAGAGATAGGATGT+GGG 0.679475 7:-86605163 MsG0780041101.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACTTATCTAGTATTTGTAT+AGG - Chr7:86605854-86605873 MsG0780041101.01.T01:CDS 20.0%
!! TATACAAATACTAGATAAGT+AGG + Chr7:86605855-86605874 None:intergenic 20.0%
! AAAAGTTTAAGGATTCTGAT+TGG - Chr7:86605706-86605725 MsG0780041101.01.T01:CDS 25.0%
! ATGATATGCATATATCATTC+AGG - Chr7:86605146-86605165 MsG0780041101.01.T01:CDS 25.0%
! ATGATCAATACATCAACAAT+GGG - Chr7:86605374-86605393 MsG0780041101.01.T01:intron 25.0%
! TAACTGATATTGAAAACTTG+AGG - Chr7:86606036-86606055 MsG0780041101.01.T01:CDS 25.0%
! TAGAGAAGTAAACAATCATA+AGG + Chr7:86605882-86605901 None:intergenic 25.0%
! TATGATCAATACATCAACAA+TGG - Chr7:86605373-86605392 MsG0780041101.01.T01:intron 25.0%
! TGTTGATATTATTTCCAGAT+TGG + Chr7:86605279-86605298 None:intergenic 25.0%
!! TTTATGTTACTTAGTATGAC+AGG - Chr7:86606004-86606023 MsG0780041101.01.T01:intron 25.0%
AATACATCAACAATGGGAAT+GGG - Chr7:86605380-86605399 MsG0780041101.01.T01:intron 30.0%
ACTAGAACCTAAACAAATAC+AGG + Chr7:86605799-86605818 None:intergenic 30.0%
ATAGGAATCATCTTAGAGAT+AGG - Chr7:86606152-86606171 MsG0780041101.01.T01:CDS 30.0%
ATCAACAAAAACAATGCAAC+TGG - Chr7:86605292-86605311 MsG0780041101.01.T01:intron 30.0%
CGTATGTTGAAAACTTAACA+AGG - Chr7:86606095-86606114 MsG0780041101.01.T01:CDS 30.0%
CTCGATAAGAGGAAAATAAA+AGG + Chr7:86605968-86605987 None:intergenic 30.0%
GATGTAACCTGTATTTGTTT+AGG - Chr7:86605789-86605808 MsG0780041101.01.T01:CDS 30.0%
TAATCATGTGCATGTTGATA+AGG - Chr7:86605408-86605427 MsG0780041101.01.T01:intron 30.0%
TACATCACATCATATGAATG+AGG - Chr7:86605516-86605535 MsG0780041101.01.T01:intron 30.0%
TTAACAAGGTTATCTGATGA+AGG - Chr7:86606109-86606128 MsG0780041101.01.T01:CDS 30.0%
TTCAACAAAAACAATGCAAC+TGG - Chr7:86605233-86605252 MsG0780041101.01.T01:CDS 30.0%
AAAACACGATACTCGATAAG+AGG + Chr7:86605979-86605998 None:intergenic 35.0%
AAGAGCTGATAGGTTAAGAA+GGG - Chr7:86605570-86605589 MsG0780041101.01.T01:intron 35.0%
AGCTGCACAATTTCAAAGAA+AGG - Chr7:86605179-86605198 MsG0780041101.01.T01:CDS 35.0%
AGTTTAAGGATTCTGATTGG+AGG - Chr7:86605709-86605728 MsG0780041101.01.T01:CDS 35.0%
ATCATCTTAGAGATAGGATG+TGG - Chr7:86606158-86606177 MsG0780041101.01.T01:CDS 35.0%
CAATACATCAACAATGGGAA+TGG - Chr7:86605379-86605398 MsG0780041101.01.T01:intron 35.0%
CTGATATTGAAAACTTGAGG+CGG - Chr7:86606039-86606058 MsG0780041101.01.T01:CDS 35.0%
GACATTTCAGATGATTCCTA+TGG - Chr7:86605673-86605692 MsG0780041101.01.T01:CDS 35.0%
GCTGCACAATTTCAAAGAAA+GGG - Chr7:86605180-86605199 MsG0780041101.01.T01:CDS 35.0%
GTTTAAGGATTCTGATTGGA+GGG - Chr7:86605710-86605729 MsG0780041101.01.T01:CDS 35.0%
TAAGAGCTGATAGGTTAAGA+AGG - Chr7:86605569-86605588 MsG0780041101.01.T01:intron 35.0%
TCATCATTCATCTACCAATC+TGG - Chr7:86605262-86605281 MsG0780041101.01.T01:CDS 35.0%
TCATCTTAGAGATAGGATGT+GGG - Chr7:86606159-86606178 MsG0780041101.01.T01:CDS 35.0%
TTTCTTTGGTAAGAGCTGAT+AGG - Chr7:86605560-86605579 MsG0780041101.01.T01:intron 35.0%
! GAAAAGCGTGGAAAAGTTTA+AGG - Chr7:86605695-86605714 MsG0780041101.01.T01:CDS 35.0%
!! CTTTGAAATTGTGCAGCTTT+GGG + Chr7:86605177-86605196 None:intergenic 35.0%
!! TCTTTGAAATTGTGCAGCTT+TGG + Chr7:86605178-86605197 None:intergenic 35.0%
ATGATTCCTATGGAAAAGCG+TGG - Chr7:86605683-86605702 MsG0780041101.01.T01:CDS 40.0%
ATGTGATGTAGACTCCTTCT+TGG + Chr7:86605506-86605525 None:intergenic 40.0%
CTGATAGGTTAAGAAGGGAT+AGG - Chr7:86605575-86605594 MsG0780041101.01.T01:intron 40.0%
TTATTTCCAGCAAGCTTGTC+TGG + Chr7:86605211-86605230 None:intergenic 40.0%
TTGTTCTCTTACCAAGTGTG+TGG - Chr7:86605630-86605649 MsG0780041101.01.T01:CDS 40.0%
TTTCGTCATCTGTGTTTGGT+GGG + Chr7:86605345-86605364 None:intergenic 40.0%
! ACTTTTCCACGCTTTTCCAT+AGG + Chr7:86605692-86605711 None:intergenic 40.0%
! TCGTTTTCGTCATCTGTGTT+TGG + Chr7:86605349-86605368 None:intergenic 40.0%
! TTTTCGTCATCTGTGTTTGG+TGG + Chr7:86605346-86605365 None:intergenic 40.0%
AACGAGGTTCCTAACCTTGA+AGG - Chr7:86606064-86606083 MsG0780041101.01.T01:CDS 45.0%
ACGAGGTTCCTAACCTTGAA+GGG - Chr7:86606065-86606084 MsG0780041101.01.T01:CDS 45.0%
AGCATGGGCTCAAGTTTCTT+TGG - Chr7:86605546-86605565 MsG0780041101.01.T01:intron 45.0%
GGTTCATGTGAGATCCAAGA+AGG - Chr7:86605489-86605508 MsG0780041101.01.T01:intron 45.0%
TTATCGCCATGCCTAATGAG+AGG - Chr7:86605738-86605757 MsG0780041101.01.T01:CDS 45.0%
! ATCTGTGTTTGGTGGGTCTT+GGG + Chr7:86605338-86605357 None:intergenic 45.0%
! TAATGAGAGGAAGCGTGGAT+GGG - Chr7:86605751-86605770 MsG0780041101.01.T01:CDS 45.0%
AAAACTTGAGGCGGTGAACG+AGG - Chr7:86606048-86606067 MsG0780041101.01.T01:CDS 50.0%
ACGCTTCCTCTCATTAGGCA+TGG + Chr7:86605747-86605766 None:intergenic 50.0%
ATGCCTAATGAGAGGAAGCG+TGG - Chr7:86605746-86605765 MsG0780041101.01.T01:CDS 50.0%
CACTCACAGTGAAAAGCGAC+AGG - Chr7:86605468-86605487 MsG0780041101.01.T01:intron 50.0%
CAGCTGAGATGGCAATGGAT+AGG - Chr7:86606134-86606153 MsG0780041101.01.T01:CDS 50.0%
CATACGAGCTCATCCCTTCA+AGG + Chr7:86606081-86606100 None:intergenic 50.0%
CATCCACGCTTCCTCTCATT+AGG + Chr7:86605752-86605771 None:intergenic 50.0%
GAGCTCATCCCTTCAAGGTT+AGG + Chr7:86606076-86606095 None:intergenic 50.0%
GGATGGGTACTTAGGCTCTA+AGG - Chr7:86605767-86605786 MsG0780041101.01.T01:CDS 50.0%
! AAGAAGGGATAGGAGTGCAG+AGG - Chr7:86605585-86605604 MsG0780041101.01.T01:intron 50.0%
! CATCTGTGTTTGGTGGGTCT+TGG + Chr7:86605339-86605358 None:intergenic 50.0%
! CTAATGAGAGGAAGCGTGGA+TGG - Chr7:86605750-86605769 MsG0780041101.01.T01:CDS 50.0%
! GAATGAGGCACTTGCAGCAT+GGG - Chr7:86605531-86605550 MsG0780041101.01.T01:intron 50.0%
! TGAATGAGGCACTTGCAGCA+TGG - Chr7:86605530-86605549 MsG0780041101.01.T01:intron 50.0%
! TGATGAAGGTGCAGCTGAGA+TGG - Chr7:86606123-86606142 MsG0780041101.01.T01:CDS 50.0%
AAGTGTGTGGCTGCTCTCGA+TGG - Chr7:86605643-86605662 MsG0780041101.01.T01:CDS 55.0%
AGGTGCAGCTGAGATGGCAA+TGG - Chr7:86606129-86606148 MsG0780041101.01.T01:CDS 55.0%
AGTGTGTGGCTGCTCTCGAT+GGG - Chr7:86605644-86605663 MsG0780041101.01.T01:CDS 55.0%
ATCGAGAGCAGCCACACACT+TGG + Chr7:86605644-86605663 None:intergenic 55.0%
GTGTGACCAGACAAGCTTGC+TGG - Chr7:86605202-86605221 MsG0780041101.01.T01:CDS 55.0%
! GGAAGCGTGGATGGGTACTT+AGG - Chr7:86605759-86605778 MsG0780041101.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 86605146 86606198 86605146 ID=MsG0780041101.01;Name=MsG0780041101.01
Chr7 mRNA 86605146 86606198 86605146 ID=MsG0780041101.01.T01;Parent=MsG0780041101.01;Name=MsG0780041101.01.T01;_AED=0.49;_eAED=0.66;_QI=0|0|0|1|0.5|0.33|3|0|203
Chr7 exon 86606029 86606198 86606029 ID=MsG0780041101.01.T01:exon:52075;Parent=MsG0780041101.01.T01
Chr7 exon 86605609 86605909 86605609 ID=MsG0780041101.01.T01:exon:52074;Parent=MsG0780041101.01.T01
Chr7 exon 86605146 86605286 86605146 ID=MsG0780041101.01.T01:exon:52073;Parent=MsG0780041101.01.T01
Chr7 CDS 86606029 86606198 86606029 ID=MsG0780041101.01.T01:cds;Parent=MsG0780041101.01.T01
Chr7 CDS 86605609 86605909 86605609 ID=MsG0780041101.01.T01:cds;Parent=MsG0780041101.01.T01
Chr7 CDS 86605146 86605286 86605146 ID=MsG0780041101.01.T01:cds;Parent=MsG0780041101.01.T01
Gene Sequence

>MsG0780041101.01.T01

ATGATATGCATATATCATTCAGGTACATCCCAAAGCTGCACAATTTCAAAGAAAGGGTGTGACCAGACAAGCTTGCTGGAAATAATATTCAACAAAAACAATGCAACTGGAGTACTTCATCATTCATCTACCAATCTGGAAATAATATCAACAAAAACAATGCAACTGGATTCATATGATGATCTACATGAAGTTGAGCACATCACTCACAGTGAAAAGCGACAGGTTCATGTGAGATCCAAGAAGGAGTCTACATCACATCATATGAATGAGGCACTTGCAGCATGGGCTCAAGTTTCTTTGGTAAGAGCTGATAGGTTAAGAAGGGATAGGAGTGCAGAGGCTACTTCGCGTGTAACTTCAGATTGTTCTCTTACCAAGTGTGTGGCTGCTCTCGATGGGCTAGAAGACATTTCAGATGATTCCTATGGAAAAGCGTGGAAAAGTTTAAGGATTCTGATTGGAGGGAATGCGGTGAACGAGGTTCCTAACCTTGAAGGGATGAGCTCGTATGTTGAAAACTTAACAAGGTTATCTGATGAAGGTGCAGCTGAGATGGCAATGGATAGGAATCATCTTAGAGATAGGATGTGGGTACATCGTCATAATTAA

Protein sequence

>MsG0780041101.01.T01

MICIYHSGTSQSCTISKKGCDQTSLLEIIFNKNNATGVLHHSSTNLEIISTKTMQLDSYDDLHEVEHITHSEKRQVHVRSKKESTSHHMNEALAAWAQVSLVRADRLRRDRSAEATSRVTSDCSLTKCVAALDGLEDISDDSYGKAWKSLRILIGGNAVNEVPNLEGMSSYVENLTRLSDEGAAEMAMDRNHLRDRMWVHRHN*