AlfalfaGEDB | Msa0211030

M. sativa cultivar zhongmu-4 / Msa0211030

KEGG: -
GO: -
PFAM: PF04788.15

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0211030	XP_013452074.1	99.483	387	2	0	1	387	1	387	0.0	806

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0211030	A0A072U8X6	99.483	387	2	0	1	387	1	387	0.0	806

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000050	Msa0211030	0.835200	1.926564e-56	-8.615850e-47
Msa0002580	Msa0211030	0.806177	9.336284e-50	-8.615850e-47
Msa0005890	Msa0211030	0.805277	1.442601e-49	-8.615850e-47
Msa0027820	Msa0211030	0.802258	6.109393e-49	-8.615850e-47
Msa0034810	Msa0211030	0.833751	4.454945e-56	-8.615850e-47
Msa0054690	Msa0211030	0.803715	3.054633e-49	-8.615850e-47
Msa0081090	Msa0211030	0.801860	7.378591e-49	-8.615850e-47
Msa0081390	Msa0211030	0.830750	2.462911e-55	-8.615850e-47
Msa0081450	Msa0211030	0.826256	2.997487e-54	-8.615850e-47
Msa0081520	Msa0211030	0.811458	6.931572e-51	-8.615850e-47
Msa0129940	Msa0211030	0.814207	1.732769e-51	-8.615850e-47
Msa0132630	Msa0211030	-0.806673	7.338506e-50	-8.615850e-47
Msa0173690	Msa0211030	0.821982	3.022753e-53	-8.615850e-47
Msa0174450	Msa0211030	0.820649	6.135244e-53	-8.615850e-47
Msa0175730	Msa0211030	0.842129	3.122094e-58	-8.615850e-47
Msa0178560	Msa0211030	0.830110	3.532246e-55	-8.615850e-47
Msa0191020	Msa0211030	0.820927	5.294732e-53	-8.615850e-47
Msa0211030	Msa0221000	0.807540	4.809345e-50	-8.615850e-47
Msa0211030	Msa0225100	0.813586	2.375339e-51	-8.615850e-47
Msa0211030	Msa0225860	0.805514	1.286741e-49	-8.615850e-47
Msa0211030	Msa0231200	0.824794	6.654053e-54	-8.615850e-47
Msa0211030	Msa0233320	0.824640	7.235617e-54	-8.615850e-47
Msa0211030	Msa0244640	0.832840	7.514587e-56	-8.615850e-47
Msa0211030	Msa0266180	0.807905	4.023105e-50	-8.615850e-47
Msa0211030	Msa0270340	0.812950	3.276308e-51	-8.615850e-47
Msa0211030	Msa0272770	0.816668	4.912432e-52	-8.615850e-47
Msa0211030	Msa0283330	0.818510	1.887702e-52	-8.615850e-47
Msa0211030	Msa0287120	0.851659	7.683375e-61	-8.615850e-47
Msa0211030	Msa0287740	0.815160	1.065762e-51	-8.615850e-47
Msa0211030	Msa0290720	0.813935	1.989326e-51	-8.615850e-47
Msa0211030	Msa0298470	0.817841	2.674451e-52	-8.615850e-47
Msa0211030	Msa0321620	0.836698	8.031895e-57	-8.615850e-47
Msa0211030	Msa0323270	0.826911	2.091796e-54	-8.615850e-47
Msa0211030	Msa0337700	0.825417	4.740991e-54	-8.615850e-47
Msa0211030	Msa0376630	0.809218	2.109762e-50	-8.615850e-47
Msa0211030	Msa0378720	0.805673	1.191569e-49	-8.615850e-47
Msa0211030	Msa0405680	0.848292	6.727362e-60	-8.615850e-47
Msa0211030	Msa0415610	0.804924	1.710377e-49	-8.615850e-47
Msa0211030	Msa0418970	0.813891	2.034589e-51	-8.615850e-47
Msa0211030	Msa0425170	0.807535	4.821496e-50	-8.615850e-47
Msa0211030	Msa0436910	0.804235	2.380967e-49	-8.615850e-47
Msa0211030	Msa0464680	0.806086	9.757896e-50	-8.615850e-47
Msa0211030	Msa0465400	0.800409	1.461164e-48	-8.615850e-47
Msa0211030	Msa0471670	0.824536	7.655225e-54	-8.615850e-47
Msa0211030	Msa0474730	0.807784	4.267865e-50	-8.615850e-47
Msa0211030	Msa0528570	0.819605	1.063706e-52	-8.615850e-47
Msa0211030	Msa0529030	0.823515	1.328820e-53	-8.615850e-47
Msa0211030	Msa0530780	0.828195	1.028893e-54	-8.615850e-47
Msa0211030	Msa0549370	0.807772	4.293052e-50	-8.615850e-47
Msa0211030	Msa0562620	0.806853	6.724224e-50	-8.615850e-47
Msa0211030	Msa0574050	0.808550	2.931339e-50	-8.615850e-47
Msa0211030	Msa0580370	0.817418	3.331703e-52	-8.615850e-47
Msa0211030	Msa0611210	0.864161	1.484003e-64	-8.615850e-47
Msa0211030	Msa0616510	0.824325	8.581995e-54	-8.615850e-47
Msa0211030	Msa0626450	0.816856	4.457148e-52	-8.615850e-47
Msa0211030	Msa0626490	0.812989	3.211345e-51	-8.615850e-47
Msa0211030	Msa0651550	0.858953	5.781637e-63	-8.615850e-47
Msa0211030	Msa0661150	0.808087	3.679681e-50	-8.615850e-47
Msa0211030	Msa0664600	0.812664	3.783246e-51	-8.615850e-47
Msa0211030	Msa0697160	0.821433	4.048093e-53	-8.615850e-47
Msa0211030	Msa0699280	0.813905	2.019685e-51	-8.615850e-47
Msa0211030	Msa0699840	0.811384	7.192298e-51	-8.615850e-47
Msa0211030	Msa0730430	0.806716	7.185478e-50	-8.615850e-47
Msa0211030	Msa0741220	0.805747	1.149576e-49	-8.615850e-47
Msa0211030	Msa0741650	0.822370	2.456822e-53	-8.615850e-47
Msa0211030	Msa0795870	0.807987	3.864119e-50	-8.615850e-47
Msa0211030	Msa0819170	0.813087	3.056746e-51	-8.615850e-47
Msa0211030	Msa0829300	0.864893	8.759431e-65	-8.615850e-47
Msa0211030	Msa0829330	0.853102	2.982887e-61	-8.615850e-47
Msa0211030	Msa0833650	0.819714	1.004610e-52	-8.615850e-47
Msa0211030	Msa0835490	0.811303	7.487851e-51	-8.615850e-47
Msa0211030	Msa0861850	0.801995	6.920170e-49	-8.615850e-47
Msa0211030	Msa0881360	0.811379	7.211874e-51	-8.615850e-47
Msa0211030	Msa0894790	0.818785	1.635256e-52	-8.615850e-47
Msa0211030	Msa0921590	0.825072	5.721894e-54	-8.615850e-47
Msa0211030	Msa0935820	0.823267	1.518891e-53	-8.615850e-47
Msa0211030	Msa0945630	0.827789	1.288448e-54	-8.615850e-47
Msa0211030	Msa0967870	0.811433	7.018061e-51	-8.615850e-47
Msa0211030	Msa1044680	0.809436	1.893697e-50	-8.615850e-47
Msa0211030	Msa1090140	0.805221	1.482297e-49	-8.615850e-47
Msa0211030	Msa1101090	0.805565	1.255478e-49	-8.615850e-47
Msa0211030	Msa1103990	0.806324	8.694963e-50	-8.615850e-47
Msa0211030	Msa1113800	0.811965	5.376735e-51	-8.615850e-47
Msa0211030	Msa1132840	0.820717	5.918596e-53	-8.615850e-47
Msa0211030	Msa1155170	-0.801117	1.047515e-48	-8.615850e-47
Msa0211030	Msa1160500	0.813609	2.346985e-51	-8.615850e-47
Msa0211030	Msa1186020	0.811973	5.356320e-51	-8.615850e-47
Msa0211030	Msa1205280	0.824105	9.665694e-54	-8.615850e-47
Msa0211030	Msa1228200	0.826917	2.085196e-54	-8.615850e-47
Msa0211030	Msa1265650	0.809918	1.492083e-50	-8.615850e-47
Msa0211030	Msa1297230	0.829014	6.523858e-55	-8.615850e-47
Msa0211030	Msa1303650	0.800406	1.463130e-48	-8.615850e-47
Msa0211030	Msa1314650	0.806993	6.278794e-50	-8.615850e-47
Msa0211030	Msa1340700	0.804863	1.761390e-49	-8.615850e-47
Msa0211030	Msa1341890	0.824409	8.197590e-54	-8.615850e-47
Msa0211030	Msa1343430	0.805986	1.024116e-49	-8.615850e-47
Msa0211030	Msa1347310	0.846584	1.981306e-59	-8.615850e-47
Msa0211030	Msa1372040	0.820621	6.225404e-53	-8.615850e-47
Msa0211030	Msa1372230	0.823933	1.060459e-53	-8.615850e-47
Msa0211030	Msa1405460	0.816747	4.714268e-52	-8.615850e-47
Msa0211030	Msa1414640	0.822564	2.214575e-53	-8.615850e-47
Msa0211030	Msa1415540	0.813968	1.956519e-51	-8.615850e-47
Msa0211030	Msa1433810	0.815095	1.101622e-51	-8.615850e-47
Msa0211030	Msa1434220	0.801793	7.615221e-49	-8.615850e-47
Msa0211030	Msa1450100	0.804426	2.172836e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0211030	MtrunA17_Chr2g0309941	99.483	387	2	0	1	387	1	387	0.0	806
Msa0211030	MtrunA17_Chr1g0152841	54.743	369	151	4	15	380	65	420	2.03e-142	413
Msa0211030	MtrunA17_Chr1g0207581	54.223	367	150	4	15	380	72	421	1.97e-139	405
Msa0211030	MtrunA17_Chr3g0134521	57.947	302	121	4	81	380	93	390	3.47e-124	364
Msa0211030	MtrunA17_Chr1g0176101	47.411	367	167	7	17	380	4	347	1.06e-112	333
Msa0211030	MtrunA17_Chr3g0112781	43.229	384	186	7	17	380	14	385	6.15e-105	316
Msa0211030	MtrunA17_Chr5g0436191	42.049	371	197	5	17	380	14	373	2.57e-96	293
Msa0211030	MtrunA17_Chr1g0154851	41.144	367	198	8	17	380	14	365	2.55e-87	269
Msa0211030	MtrunA17_Chr5g0403841	39.782	367	204	9	17	380	23	375	1.22e-83	259
Msa0211030	MtrunA17_Chr3g0141481	39.402	368	203	8	17	379	21	373	6.22e-82	255

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0211030	AT1G79420.1	69.042	407	101	10	1	385	1	404	0.0	548
Msa0211030	AT1G49840.1	53.825	366	158	5	17	380	86	442	7.20e-139	405
Msa0211030	AT3G19540.1	55.786	337	138	5	45	380	108	434	1.55e-135	396
Msa0211030	AT1G27690.1	51.762	369	165	6	17	380	49	409	4.01e-128	375
Msa0211030	AT5G05840.1	44.772	373	197	3	17	380	14	386	1.94e-108	325
Msa0211030	AT5G06610.1	47.340	376	161	11	15	380	2	350	1.63e-103	310
Msa0211030	AT5G66740.1	44.444	369	184	9	17	380	14	366	8.91e-92	280
Msa0211030	AT3G55720.1	38.642	383	210	9	17	380	14	390	2.91e-82	258
Msa0211030	AT1G75160.1	39.529	382	207	9	17	380	16	391	5.62e-80	251

Find 129 sgRNAs with CRISPR-Local

Find 240 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGCTAAGACACAAGATTTA+AGG	0.141304	2_1:-51728352	Msa0211030:CDS
CCCATTACGTCGCATTATTC+AGG	0.207004	2_1:-51727529	Msa0211030:intron
AAGAAAGTGATGCAGTTTAT+TGG	0.222255	2_1:-51725622	Msa0211030:CDS
AGGTTATTGCTTGGTGTCTT+AGG	0.229331	2_1:-51728332	Msa0211030:CDS
TAGTGAGATGTGAGTCTTCT+AGG	0.242266	2_1:+51725657	None:intergenic
CCCCTTCTTCTTGCATTTCA+GGG	0.262239	2_1:+51728477	None:intergenic
CCTTCCATCAATGGTGTTAA+TGG	0.267531	2_1:+51728506	None:intergenic
AAAGCTCTTGGGAAGCTATA+AGG	0.275008	2_1:-51728445	Msa0211030:CDS
AATAAGCTTGCTGTGCTCTT+TGG	0.308806	2_1:+51725848	None:intergenic
AGAAAGTGATGCAGTTTATT+GGG	0.328630	2_1:-51725621	Msa0211030:CDS
GGGACAAGAAGTAGTGAAAA+TGG	0.331520	2_1:-51727699	Msa0211030:CDS
GGGAAGAATGTAAAGTGTTT+GGG	0.349866	2_1:-51727720	Msa0211030:CDS
AGGGACCCGCAGAGGTAATA+AGG	0.351801	2_1:-51725727	Msa0211030:CDS
AGGGAAGAATGTAAAGTGTT+TGG	0.360355	2_1:-51727721	Msa0211030:CDS
TCTTCTTGCATTTCAGGGTC+AGG	0.369373	2_1:+51728482	None:intergenic
GAACCAGGATGGAAGAGATT+TGG	0.370230	2_1:-51725478	Msa0211030:CDS
AATTCAAGTGACCACATTCC+TGG	0.370644	2_1:+51727651	None:intergenic
CCTGAATAATGCGACGTAAT+GGG	0.370807	2_1:+51727529	None:intergenic
TCAACATAGGTAGTAGCATT+AGG	0.375453	2_1:-51725332	Msa0211030:three_prime_UTR
GTAATAAGGCACATTCTATA+TGG	0.375738	2_1:-51725713	Msa0211030:CDS
AATGGAATAAATTGATCAAT+TGG	0.382219	2_1:-51725129	Msa0211030:three_prime_UTR
ACCTGAATAATGCGACGTAA+TGG	0.389760	2_1:+51727528	None:intergenic
AAGCTCTTGGGAAGCTATAA+GGG	0.396989	2_1:-51728444	Msa0211030:CDS
TACTACCAGCAACAATCTTA+TGG	0.403414	2_1:+51727616	None:intergenic
ACAGCCATTAACACCATTGA+TGG	0.406142	2_1:-51728510	Msa0211030:CDS
GGATCATGATCTGAAGGAAT+AGG	0.416055	2_1:+51728296	None:intergenic
TGTGAATTGTAACTTTACTT+TGG	0.420626	2_1:-51725283	Msa0211030:three_prime_UTR
CGTTGATTGCTTCGTGCTAA+AGG	0.421358	2_1:-51725786	Msa0211030:CDS
GGGTATAATTAAAGTATTGG+TGG	0.421483	2_1:-51725257	Msa0211030:three_prime_UTR
AGGAATGTGGTCACTTGAAT+TGG	0.425234	2_1:-51727649	Msa0211030:CDS
AGCACGTTGGCGTCAGAGTT+GGG	0.426820	2_1:-51728537	Msa0211030:CDS
ATCTTGTGTCTTAGCATGAA+TGG	0.430849	2_1:+51728358	None:intergenic
CAACAATATTTAGCTGCAAC+AGG	0.431886	2_1:-51727828	Msa0211030:CDS
AATTGTAACTTTACTTTGGA+GGG	0.441146	2_1:-51725279	Msa0211030:three_prime_UTR
TGCGACGTAATGGGCGTTGT+GGG	0.443411	2_1:+51727538	None:intergenic
GGTGGCCATAAGATTGTTGC+TGG	0.448162	2_1:-51727621	Msa0211030:CDS
TGGTCACTTGAATTGGTTGT+TGG	0.455517	2_1:-51727642	Msa0211030:CDS
ATCGCGACGGTGTTTCGATT+CGG	0.460383	2_1:-51725524	Msa0211030:CDS
ACTGCATCACTTTCTTGTGT+TGG	0.469166	2_1:+51725629	None:intergenic
TGTGAAACGGAGATATCATC+AGG	0.472665	2_1:-51727741	Msa0211030:CDS
CAGCACGTTGGCGTCAGAGT+TGG	0.474044	2_1:-51728538	Msa0211030:CDS
AAAATGGTTGCTTTGTACTT+TGG	0.478913	2_1:-51727683	Msa0211030:CDS
ACCCTGAAATGCAAGAAGAA+GGG	0.478946	2_1:-51728478	Msa0211030:CDS
TTCACAAATGCACAATGCTT+AGG	0.482112	2_1:-51725827	Msa0211030:CDS
GTATAATTAAAGTATTGGTG+GGG	0.483756	2_1:-51725255	Msa0211030:three_prime_UTR
GTACTTTGGCAAATGTTGCC+AGG	0.484962	2_1:-51727669	Msa0211030:CDS
ATCACCAATGCTACTACCTA+TGG	0.486046	2_1:+51725592	None:intergenic
GGTGATTATAGAGACGTTGA+TGG	0.486306	2_1:-51725575	Msa0211030:CDS
TCACTTGAATTGGTTGTTGG+TGG	0.488837	2_1:-51727639	Msa0211030:CDS
TATAATTAAAGTATTGGTGG+GGG	0.491405	2_1:-51725254	Msa0211030:three_prime_UTR
GGGTCAGGACCTTCCATCAA+TGG	0.491906	2_1:+51728497	None:intergenic
GACCCTGAAATGCAAGAAGA+AGG	0.492624	2_1:-51728479	Msa0211030:CDS
ATCAATGGTGTTAATGGCTG+TGG	0.494989	2_1:+51728512	None:intergenic
ATGTGCCTTATTACCTCTGC+GGG	0.498139	2_1:+51725722	None:intergenic
GTGAGTCTTCTAGGTATATG+AGG	0.499393	2_1:+51725666	None:intergenic
GAATTGTAACTTTACTTTGG+AGG	0.511197	2_1:-51725280	Msa0211030:three_prime_UTR
TTAGGTACTCATGCTGCTAA+AGG	0.514234	2_1:-51727561	Msa0211030:CDS
AGGATGTTTGAAACAACAAA+AGG	0.514313	2_1:-51727808	Msa0211030:CDS
TGGAGACATACCCCTTGGTT+AGG	0.514573	2_1:-51727579	Msa0211030:CDS
AAGATTGTTGCTGGTAGTAA+TGG	0.516674	2_1:-51727612	Msa0211030:CDS
TGATGATTGTGTGATGAGAA+TGG	0.516827	2_1:+51728603	None:intergenic
CAAGATTTAAGGTTATTGCT+TGG	0.523130	2_1:-51728341	Msa0211030:CDS
ATTAAACATAACATCATCAA+TGG	0.526065	2_1:+51725454	None:intergenic
TGCTTAGGTGAAAATCGAAT+CGG	0.527036	2_1:-51725812	Msa0211030:CDS
TGGTGTCTTAGGTTGTCCCT+TGG	0.529301	2_1:-51728321	Msa0211030:CDS
GGTCCAAATCTCTTCCATCC+TGG	0.529322	2_1:+51725475	None:intergenic
TAATGTACCTGGACTCTCCA+TGG	0.530964	2_1:-51725435	Msa0211030:CDS
TTGTAACTTTACTTTGGAGG+GGG	0.534623	2_1:-51725277	Msa0211030:three_prime_UTR
GTGATGAAATGTTTCCACTA+GGG	0.538316	2_1:+51728396	None:intergenic
GAAGATGATTTGTTGTGAAA+CGG	0.538802	2_1:-51727754	Msa0211030:CDS
CTCAAATGACAAAATCAAGA+GGG	0.541601	2_1:-51725153	Msa0211030:three_prime_UTR
GGGATCTTGATTGCACATCA+AGG	0.542348	2_1:-51725554	Msa0211030:CDS
TAGAAGACTCACATCTCACT+AGG	0.544543	2_1:-51725655	Msa0211030:CDS
TGACAAAATCAAGAGGGGAA+TGG	0.546304	2_1:-51725147	Msa0211030:three_prime_UTR
GGTATAATTAAAGTATTGGT+GGG	0.546661	2_1:-51725256	Msa0211030:three_prime_UTR
ATGCGACGTAATGGGCGTTG+TGG	0.546837	2_1:+51727537	None:intergenic
TGATGAAATGTTTCCACTAG+GGG	0.553171	2_1:+51728397	None:intergenic
CGATGCAGCATAGTAGAACC+AGG	0.559000	2_1:-51725493	Msa0211030:CDS
ATCTGAAGGAATAGGAGCCA+AGG	0.559961	2_1:+51728304	None:intergenic
ATATTGTTGTATGATGTACT+TGG	0.563693	2_1:+51727842	None:intergenic
CCCTGAAATGCAAGAAGAAG+GGG	0.565828	2_1:-51728477	Msa0211030:CDS
GTGATTATAGAGACGTTGAT+GGG	0.566846	2_1:-51725574	Msa0211030:CDS
CTTGGGAAGCTATAAGGGAA+TGG	0.567830	2_1:-51728439	Msa0211030:CDS
AGAATAAGAATGAAATGAAG+AGG	0.571508	2_1:+51728627	None:intergenic
AAATGATCCATGGAGAGTCC+AGG	0.573862	2_1:+51725428	None:intergenic
TTAATCATCCTCCACACGAA+TGG	0.574193	2_1:-51728570	Msa0211030:exon
GCTCAGAAAATTTCCCCTAG+TGG	0.576631	2_1:-51728410	Msa0211030:CDS
AAAGCAGGATCATGATCTGA+AGG	0.576935	2_1:+51728290	None:intergenic
ACCAATGCTACTACCTATGG+TGG	0.585127	2_1:+51725595	None:intergenic
ATTGTAACTTTACTTTGGAG+GGG	0.587507	2_1:-51725278	Msa0211030:three_prime_UTR
ATATTGTCAAGAATATCAGC+AGG	0.587919	2_1:+51725398	None:intergenic
TTGTGATGAATACTTAAAGC+AGG	0.589050	2_1:+51728275	None:intergenic
GATGTTATGTTTAATGTACC+TGG	0.591896	2_1:-51725446	Msa0211030:CDS
GACTGTGATAGAAAGAAGTG+AGG	0.592300	2_1:-51725747	Msa0211030:CDS
GAATCGAAACACCGTCGCGA+TGG	0.595803	2_1:+51725526	None:intergenic
ACCACCATAGGTAGTAGCAT+TGG	0.603738	2_1:-51725596	Msa0211030:CDS
GTAGTTAGAGCTTACAAATG+GGG	0.607349	2_1:+51728238	None:intergenic
GTGTATTGGGAGATCAACAT+AGG	0.608257	2_1:-51725345	Msa0211030:three_prime_UTR
AAGAAGTGAGGGACCCGCAG+AGG	0.611234	2_1:-51725735	Msa0211030:CDS
CAGCATGAGTACCTAACCAA+GGG	0.614923	2_1:+51727568	None:intergenic
TGTGGAGGATGATTAAATGT+AGG	0.619356	2_1:+51728577	None:intergenic
GCTCAAATGACAAAATCAAG+AGG	0.622958	2_1:-51725154	Msa0211030:three_prime_UTR
GTTTATTGGGAAACCACCAT+AGG	0.625539	2_1:-51725608	Msa0211030:CDS
GCAGCATAGTAGAACCAGGA+TGG	0.627485	2_1:-51725489	Msa0211030:CDS
AGGAGGAATGAAATGATCCA+TGG	0.631322	2_1:+51725418	None:intergenic
GGAAGAATGTAAAGTGTTTG+GGG	0.632161	2_1:-51727719	Msa0211030:CDS
TGTGATGAAATGTTTCCACT+AGG	0.632312	2_1:+51728395	None:intergenic
CTGTGTGGAGACATACCCCT+TGG	0.632590	2_1:-51727584	Msa0211030:CDS
TCTTGATTGCACATCAAGGA+CGG	0.635806	2_1:-51725550	Msa0211030:CDS
AGAAGACTCACATCTCACTA+GGG	0.641426	2_1:-51725654	Msa0211030:CDS
GGCAAATGTTGCCAGGAATG+TGG	0.644887	2_1:-51727662	Msa0211030:CDS
TCTGAAGGAATAGGAGCCAA+GGG	0.645174	2_1:+51728305	None:intergenic
TCAAGGACGGTCCATCGCGA+CGG	0.645519	2_1:-51725537	Msa0211030:CDS
GGAGGATGATTAAATGTAGG+TGG	0.647132	2_1:+51728580	None:intergenic
CCATTAACACCATTGATGGA+AGG	0.654115	2_1:-51728506	Msa0211030:CDS
ATAATTAAAGTATTGGTGGG+GGG	0.656303	2_1:-51725253	Msa0211030:three_prime_UTR
TCTTGAAGCCATTCGTGTGG+AGG	0.660509	2_1:+51728562	None:intergenic
ACTGTGATAGAAAGAAGTGA+GGG	0.663122	2_1:-51725746	Msa0211030:CDS
TCAAATGACAAAATCAAGAG+GGG	0.667328	2_1:-51725152	Msa0211030:three_prime_UTR
GTAGTAATGGTAAGACTGTG+TGG	0.669223	2_1:-51727599	Msa0211030:CDS
CCTGAAATGCAAGAAGAAGG+GGG	0.673879	2_1:-51728476	Msa0211030:CDS
TTGTCAAGAATATCAGCAGG+AGG	0.674372	2_1:+51725401	None:intergenic
GCAGCATGAGTACCTAACCA+AGG	0.675347	2_1:+51727567	None:intergenic
TGGCTTCAAGATCAGCACGT+TGG	0.693455	2_1:-51728550	Msa0211030:CDS
AATGTGCCTTATTACCTCTG+CGG	0.693680	2_1:+51725721	None:intergenic
GTGAAACGGAGATATCATCA+GGG	0.693868	2_1:-51727740	Msa0211030:CDS
TGATCTTGAAGCCATTCGTG+TGG	0.705973	2_1:+51728559	None:intergenic
AGCATGAGTACCTAACCAAG+GGG	0.728447	2_1:+51727569	None:intergenic
AAACATGTATGCAACTGGGG+TGG	0.744117	2_1:-51727778	Msa0211030:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTATTTTGGTATAAATAAT+TGG	-	chr2_1:51725598-51725617	Msa0211030:CDS	10.0%
!!	TTAAAAATAAATTTATTCCA+TGG	+	chr2_1:51725661-51725680	None:intergenic	10.0%
!!	ATTACATTTATAATACAATA+TGG	-	chr2_1:51727549-51727568	Msa0211030:CDS	10.0%
!!!	TTTTTGTTAACTTTTTTTAA+AGG	-	chr2_1:51728478-51728497	Msa0211030:CDS	10.0%
!!!	GCATTTTTTAACATTTATTT+TGG	-	chr2_1:51725585-51725604	Msa0211030:CDS	15.0%
!!!	AGTCACTTTTTTTTTTTAAT+AGG	-	chr2_1:51725910-51725929	Msa0211030:intron	15.0%
!!!	ATGATTTTCTTTTTATGTTA+GGG	+	chr2_1:51726502-51726521	None:intergenic	15.0%
!!!	TTCATCATTTTTTATTATCA+TGG	+	chr2_1:51728422-51728441	None:intergenic	15.0%
!!!	ATTTCATCACAATCATTTTA+TGG	-	chr2_1:51725404-51725423	Msa0211030:CDS	20.0%
!!	AATAAATTTATTCCATGGTT+AGG	+	chr2_1:51725656-51725675	None:intergenic	20.0%
!!	ATATAAATGCATATTTCGAT+TGG	-	chr2_1:51725712-51725731	Msa0211030:CDS	20.0%
!!	TATAAATGCATATTTCGATT+GGG	-	chr2_1:51725713-51725732	Msa0211030:CDS	20.0%
!!!	TTTTTACGTAAAAATGTGAT+CGG	+	chr2_1:51726307-51726326	None:intergenic	20.0%
!!	TTATTTAGTGTAACTTTCTA+AGG	-	chr2_1:51726415-51726434	Msa0211030:intron	20.0%
!!!	AATTGCCTTAAATTTTTGTT+AGG	-	chr2_1:51726442-51726461	Msa0211030:intron	20.0%
!!	GTTTACCTAACAAAAATTTA+AGG	+	chr2_1:51726450-51726469	None:intergenic	20.0%
!!	TATGTTAGGGTAAAAAAATT+AGG	+	chr2_1:51726489-51726508	None:intergenic	20.0%
!!!	CATGATTTTCTTTTTATGTT+AGG	+	chr2_1:51726503-51726522	None:intergenic	20.0%
!!	TTTGTCATTGAAATTGTAAT+CGG	+	chr2_1:51726725-51726744	None:intergenic	20.0%
!!!	CTAAGTTAATATCTTTCTTT+TGG	+	chr2_1:51726862-51726881	None:intergenic	20.0%
!!	CAAAAGAAAGATATTAACTT+AGG	-	chr2_1:51726860-51726879	Msa0211030:intron	20.0%
!!!	ACTATAGTTCAAAATTTGTT+TGG	+	chr2_1:51726966-51726985	None:intergenic	20.0%
!!	GTATATTCTATATTTGTGTT+TGG	-	chr2_1:51726989-51727008	Msa0211030:intron	20.0%
!!!	TAGATTTCATTTGTTTCTTA+GGG	-	chr2_1:51727031-51727050	Msa0211030:intron	20.0%
!!!	TACAAACGTGAAATTTTATT+TGG	-	chr2_1:51727207-51727226	Msa0211030:intron	20.0%
!!	CAAGTACAAATAATGTTAAA+GGG	+	chr2_1:51727455-51727474	None:intergenic	20.0%
!!	ACAAGTACAAATAATGTTAA+AGG	+	chr2_1:51727456-51727475	None:intergenic	20.0%
!!!	TTCTTTTTGTTAACAATGTT+TGG	-	chr2_1:51727476-51727495	Msa0211030:intron	20.0%
!!	CTACCTTATTAAAAAAAGTT+TGG	-	chr2_1:51727638-51727657	Msa0211030:CDS	20.0%
!!!	TAGCCAAACTTTTTTTAATA+AGG	+	chr2_1:51727644-51727663	None:intergenic	20.0%
!!!	TTTAACAACTTGTATTTTGA+TGG	-	chr2_1:51727895-51727914	Msa0211030:intron	20.0%
!!	ATTAAACATAACATCATCAA+TGG	+	chr2_1:51728336-51728355	None:intergenic	20.0%
!!	TAAAAAATGATGAAGTGTAT+TGG	-	chr2_1:51728428-51728447	Msa0211030:CDS	20.0%
!!	AAAAAATGATGAAGTGTATT+GGG	-	chr2_1:51728429-51728448	Msa0211030:CDS	20.0%
!!	AATGGAATAAATTGATCAAT+TGG	-	chr2_1:51728658-51728677	Msa0211030:five_prime_UTR	20.0%
!	AGAATAAGAATGAAATGAAG+AGG	+	chr2_1:51725163-51725182	None:intergenic	25.0%
!!!	TGTAGTATTTTTCAAAATCG+TGG	+	chr2_1:51725767-51725786	None:intergenic	25.0%
!	ATATTGTTGTATGATGTACT+TGG	+	chr2_1:51725948-51725967	None:intergenic	25.0%
!	AATAAAAACATGTATGCAAC+TGG	-	chr2_1:51726004-51726023	Msa0211030:intron	25.0%
!	ATAAAAACATGTATGCAACT+GGG	-	chr2_1:51726005-51726024	Msa0211030:intron	25.0%
!	TTAATATAGACCAGATACAT+TGG	+	chr2_1:51726355-51726374	None:intergenic	25.0%
!	GAAAATCATGTTAAGTAATG+TGG	-	chr2_1:51726513-51726532	Msa0211030:intron	25.0%
!	TTAAAAGATGTGCTAACATA+TGG	-	chr2_1:51726823-51726842	Msa0211030:intron	25.0%
!!	GTTAATATCTTTCTTTTGGT+TGG	+	chr2_1:51726858-51726877	None:intergenic	25.0%
!!	TAAAAGCAAGAAATGACTTT+TGG	-	chr2_1:51726922-51726941	Msa0211030:intron	25.0%
!!	TATATTTGTGTTTGGTTTCT+TGG	-	chr2_1:51726997-51727016	Msa0211030:intron	25.0%
!!	GTAGATTTCATTTGTTTCTT+AGG	-	chr2_1:51727030-51727049	Msa0211030:intron	25.0%
!!	AGATTTCATTTGTTTCTTAG+GGG	-	chr2_1:51727032-51727051	Msa0211030:intron	25.0%
!	TTTCAATCTCTTTCTTTGTT+TGG	+	chr2_1:51727089-51727108	None:intergenic	25.0%
!	AAAATCCCAATGAATATTGA+AGG	-	chr2_1:51727136-51727155	Msa0211030:intron	25.0%
!	AAATCCCAATGAATATTGAA+GGG	-	chr2_1:51727137-51727156	Msa0211030:intron	25.0%
!	TATACCCTTCAATATTCATT+GGG	+	chr2_1:51727144-51727163	None:intergenic	25.0%
!	ATATACCCTTCAATATTCAT+TGG	+	chr2_1:51727145-51727164	None:intergenic	25.0%
!	AAAGGATAATTCATTCTTCA+GGG	+	chr2_1:51727350-51727369	None:intergenic	25.0%
!	AAAAGGATAATTCATTCTTC+AGG	+	chr2_1:51727351-51727370	None:intergenic	25.0%
!	AAAGAAAAAGCAAAAGCAAA+AGG	+	chr2_1:51727368-51727387	None:intergenic	25.0%
!	TAATGTTAAAGGGAATATGA+AGG	+	chr2_1:51727445-51727464	None:intergenic	25.0%
!	AGTTCTTAATACTCATAGTA+GGG	+	chr2_1:51727612-51727631	None:intergenic	25.0%
!	TAGTTCTTAATACTCATAGT+AGG	+	chr2_1:51727613-51727632	None:intergenic	25.0%
!!!	ATTTTGATGGTGCAAATATT+AGG	-	chr2_1:51727908-51727927	Msa0211030:intron	25.0%
!!!	TTTTGATGGTGCAAATATTA+GGG	-	chr2_1:51727909-51727928	Msa0211030:intron	25.0%
!	TGTGAATTGTAACTTTACTT+TGG	-	chr2_1:51728504-51728523	Msa0211030:CDS	25.0%
!	AATTGTAACTTTACTTTGGA+GGG	-	chr2_1:51728508-51728527	Msa0211030:CDS	25.0%
!!!	GGTATAATTAAAGTATTGGT+GGG	-	chr2_1:51728531-51728550	Msa0211030:CDS	25.0%
!!!	GTATAATTAAAGTATTGGTG+GGG	-	chr2_1:51728532-51728551	Msa0211030:CDS	25.0%
!!!	TATAATTAAAGTATTGGTGG+GGG	-	chr2_1:51728533-51728552	Msa0211030:CDS	25.0%
!!	TTTTAGAAAGTGGATGGTTA+GGG	+	chr2_1:51725140-51725159	None:intergenic	30.0%
!!!	AATGAAGAGGTTTTAGAAAG+TGG	+	chr2_1:51725150-51725169	None:intergenic	30.0%
	ATGCTAAGACACAAGATTTA+AGG	-	chr2_1:51725435-51725454	Msa0211030:CDS	30.0%
!	CAAGATTTAAGGTTATTGCT+TGG	-	chr2_1:51725446-51725465	Msa0211030:CDS	30.0%
	TTGTGATGAATACTTAAAGC+AGG	+	chr2_1:51725515-51725534	None:intergenic	30.0%
	AGTAGTTAGAGCTTACAAAT+GGG	+	chr2_1:51725553-51725572	None:intergenic	30.0%
	TAGTAGTTAGAGCTTACAAA+TGG	+	chr2_1:51725554-51725573	None:intergenic	30.0%
!!	AGTATTTTTCAAAATCGTGG+TGG	+	chr2_1:51725764-51725783	None:intergenic	30.0%
	TTATCCTATCTAGTAAGATG+TGG	-	chr2_1:51725876-51725895	Msa0211030:intron	30.0%
!	AGGATGTTTGAAACAACAAA+AGG	-	chr2_1:51725979-51725998	Msa0211030:intron	30.0%
	TAAAAACATGTATGCAACTG+GGG	-	chr2_1:51726006-51726025	Msa0211030:intron	30.0%
!!	GAAGATGATTTGTTGTGAAA+CGG	-	chr2_1:51726033-51726052	Msa0211030:intron	30.0%
!	AAAATGGTTGCTTTGTACTT+TGG	-	chr2_1:51726104-51726123	Msa0211030:intron	30.0%
	TACTGAATAACCAATGTATC+TGG	-	chr2_1:51726342-51726361	Msa0211030:intron	30.0%
!	GATTTCATTTGTTTCTTAGG+GGG	-	chr2_1:51727033-51727052	Msa0211030:intron	30.0%
	ACGTTTGTAGTGTAAAGTAA+GGG	+	chr2_1:51727196-51727215	None:intergenic	30.0%
	AAGGATAATTCATTCTTCAG+GGG	+	chr2_1:51727349-51727368	None:intergenic	30.0%
!	AATATGAAGGTATGAAAGCA+GGG	+	chr2_1:51727432-51727451	None:intergenic	30.0%
!	GAAAATACTAACGAGTGTTT+TGG	+	chr2_1:51727801-51727820	None:intergenic	30.0%
	GTAATAAGGCACATTCTATA+TGG	-	chr2_1:51728074-51728093	Msa0211030:intron	30.0%
	AAGAAAGTGATGCAGTTTAT+TGG	-	chr2_1:51728165-51728184	Msa0211030:intron	30.0%
	AGAAAGTGATGCAGTTTATT+GGG	-	chr2_1:51728166-51728185	Msa0211030:intron	30.0%
	GATGTTATGTTTAATGTACC+TGG	-	chr2_1:51728341-51728360	Msa0211030:CDS	30.0%
	ATATTGTCAAGAATATCAGC+AGG	+	chr2_1:51728392-51728411	None:intergenic	30.0%
	GAATTGTAACTTTACTTTGG+AGG	-	chr2_1:51728507-51728526	Msa0211030:CDS	30.0%
!	ATTGTAACTTTACTTTGGAG+GGG	-	chr2_1:51728509-51728528	Msa0211030:CDS	30.0%
!	AGGGGGTATAATTAAAGTAT+TGG	-	chr2_1:51728527-51728546	Msa0211030:CDS	30.0%
!!	GGGTATAATTAAAGTATTGG+TGG	-	chr2_1:51728530-51728549	Msa0211030:CDS	30.0%
!!	ATAATTAAAGTATTGGTGGG+GGG	-	chr2_1:51728534-51728553	Msa0211030:CDS	30.0%
	CTCAAATGACAAAATCAAGA+GGG	-	chr2_1:51728634-51728653	Msa0211030:five_prime_UTR	30.0%
	TCAAATGACAAAATCAAGAG+GGG	-	chr2_1:51728635-51728654	Msa0211030:five_prime_UTR	30.0%
!!!	GTTTTAGAAAGTGGATGGTT+AGG	+	chr2_1:51725141-51725160	None:intergenic	35.0%
!!!	AAGAGGTTTTAGAAAGTGGA+TGG	+	chr2_1:51725146-51725165	None:intergenic	35.0%
	TGATGATTGTGTGATGAGAA+TGG	+	chr2_1:51725187-51725206	None:intergenic	35.0%
	TGTGGAGGATGATTAAATGT+AGG	+	chr2_1:51725213-51725232	None:intergenic	35.0%
	TGATGAAATGTTTCCACTAG+GGG	+	chr2_1:51725393-51725412	None:intergenic	35.0%
	GTGATGAAATGTTTCCACTA+GGG	+	chr2_1:51725394-51725413	None:intergenic	35.0%
	TGTGATGAAATGTTTCCACT+AGG	+	chr2_1:51725395-51725414	None:intergenic	35.0%
	ATCTTGTGTCTTAGCATGAA+TGG	+	chr2_1:51725432-51725451	None:intergenic	35.0%
	GTAGTTAGAGCTTACAAATG+GGG	+	chr2_1:51725552-51725571	None:intergenic	35.0%
	CAACAATATTTAGCTGCAAC+AGG	-	chr2_1:51725959-51725978	Msa0211030:intron	35.0%
!	AGGGAAGAATGTAAAGTGTT+TGG	-	chr2_1:51726066-51726085	Msa0211030:intron	35.0%
!	GGGAAGAATGTAAAGTGTTT+GGG	-	chr2_1:51726067-51726086	Msa0211030:intron	35.0%
!	GGAAGAATGTAAAGTGTTTG+GGG	-	chr2_1:51726068-51726087	Msa0211030:intron	35.0%
	TACTACCAGCAACAATCTTA+TGG	+	chr2_1:51726174-51726193	None:intergenic	35.0%
	AAGATTGTTGCTGGTAGTAA+TGG	-	chr2_1:51726175-51726194	Msa0211030:intron	35.0%
	TTACGTAAAAATGTGATCGG+AGG	+	chr2_1:51726304-51726323	None:intergenic	35.0%
	TGTGCTTTCAATTCCAATAG+TGG	-	chr2_1:51726770-51726789	Msa0211030:intron	35.0%
	CATTTAAGTGCTTCCACTAT+TGG	+	chr2_1:51726786-51726805	None:intergenic	35.0%
!	TTTGTGTTTGGTTTCTTGGT+TGG	-	chr2_1:51727001-51727020	Msa0211030:intron	35.0%
	CACGTTTGTAGTGTAAAGTA+AGG	+	chr2_1:51727197-51727216	None:intergenic	35.0%
	TAGTTAGTGAGTCATGATCT+TGG	-	chr2_1:51727284-51727303	Msa0211030:intron	35.0%
	AGTTAGTGAGTCATGATCTT+GGG	-	chr2_1:51727285-51727304	Msa0211030:intron	35.0%
!	GAATATGAAGGTATGAAAGC+AGG	+	chr2_1:51727433-51727452	None:intergenic	35.0%
	TTGTTAACAATGTTTGGCCT+AGG	-	chr2_1:51727482-51727501	Msa0211030:intron	35.0%
	GATAACGTTTCATTCCCTAT+AGG	-	chr2_1:51727504-51727523	Msa0211030:intron	35.0%
	AAAAAATGGCTGCTCCTATA+GGG	+	chr2_1:51727521-51727540	None:intergenic	35.0%
	AAAAAAATGGCTGCTCCTAT+AGG	+	chr2_1:51727522-51727541	None:intergenic	35.0%
	TAGGGACAGACATTACAATA+TGG	+	chr2_1:51727594-51727613	None:intergenic	35.0%
	ATGGTGCAAATATTAGGGTT+TGG	-	chr2_1:51727914-51727933	Msa0211030:intron	35.0%
	TTCACAAATGCACAATGCTT+AGG	-	chr2_1:51727960-51727979	Msa0211030:intron	35.0%
	TGCTTAGGTGAAAATCGAAT+CGG	-	chr2_1:51727975-51727994	Msa0211030:intron	35.0%
	ACTGTGATAGAAAGAAGTGA+GGG	-	chr2_1:51728041-51728060	Msa0211030:intron	35.0%
!!!	ATATGAGGAGTCCACTTTTT+TGG	+	chr2_1:51728109-51728128	None:intergenic	35.0%
	GTGATTATAGAGACGTTGAT+GGG	-	chr2_1:51728213-51728232	Msa0211030:intron	35.0%
	TCAACATAGGTAGTAGCATT+AGG	-	chr2_1:51728455-51728474	Msa0211030:CDS	35.0%
!	TTGTAACTTTACTTTGGAGG+GGG	-	chr2_1:51728510-51728529	Msa0211030:CDS	35.0%
	GCTCAAATGACAAAATCAAG+AGG	-	chr2_1:51728633-51728652	Msa0211030:five_prime_UTR	35.0%
	GGAGGATGATTAAATGTAGG+TGG	+	chr2_1:51725210-51725229	None:intergenic	40.0%
	TTAATCATCCTCCACACGAA+TGG	-	chr2_1:51725217-51725236	Msa0211030:three_prime_UTR	40.0%
!	ATCAATGGTGTTAATGGCTG+TGG	+	chr2_1:51725278-51725297	None:intergenic	40.0%
	ACAGCCATTAACACCATTGA+TGG	-	chr2_1:51725277-51725296	Msa0211030:three_prime_UTR	40.0%
!	CCTTCCATCAATGGTGTTAA+TGG	+	chr2_1:51725284-51725303	None:intergenic	40.0%
	CCATTAACACCATTGATGGA+AGG	-	chr2_1:51725281-51725300	Msa0211030:three_prime_UTR	40.0%
	ACCCTGAAATGCAAGAAGAA+GGG	-	chr2_1:51725309-51725328	Msa0211030:three_prime_UTR	40.0%
	GGGCAAAAAAGAAAGCTCTT+GGG	-	chr2_1:51725331-51725350	Msa0211030:three_prime_UTR	40.0%
	AAAGCTCTTGGGAAGCTATA+AGG	-	chr2_1:51725342-51725361	Msa0211030:three_prime_UTR	40.0%
	AAGCTCTTGGGAAGCTATAA+GGG	-	chr2_1:51725343-51725362	Msa0211030:three_prime_UTR	40.0%
!!	AGGTTATTGCTTGGTGTCTT+AGG	-	chr2_1:51725455-51725474	Msa0211030:CDS	40.0%
	GGATCATGATCTGAAGGAAT+AGG	+	chr2_1:51725494-51725513	None:intergenic	40.0%
	AAAGCAGGATCATGATCTGA+AGG	+	chr2_1:51725500-51725519	None:intergenic	40.0%
	GCTCATTTAGTACCTAACCA+TGG	-	chr2_1:51725641-51725660	Msa0211030:CDS	40.0%
	CCAACCACATCTTACTAGAT+AGG	+	chr2_1:51725883-51725902	None:intergenic	40.0%
	CCTATCTAGTAAGATGTGGT+TGG	-	chr2_1:51725880-51725899	Msa0211030:intron	40.0%
	TGTGAAACGGAGATATCATC+AGG	-	chr2_1:51726046-51726065	Msa0211030:intron	40.0%
	GTGAAACGGAGATATCATCA+GGG	-	chr2_1:51726047-51726066	Msa0211030:intron	40.0%
	GGGACAAGAAGTAGTGAAAA+TGG	-	chr2_1:51726088-51726107	Msa0211030:intron	40.0%
	AATTCAAGTGACCACATTCC+TGG	+	chr2_1:51726139-51726158	None:intergenic	40.0%
	AGGAATGTGGTCACTTGAAT+TGG	-	chr2_1:51726138-51726157	Msa0211030:intron	40.0%
	TGGTCACTTGAATTGGTTGT+TGG	-	chr2_1:51726145-51726164	Msa0211030:intron	40.0%
	TCACTTGAATTGGTTGTTGG+TGG	-	chr2_1:51726148-51726167	Msa0211030:intron	40.0%
	GTAGTAATGGTAAGACTGTG+TGG	-	chr2_1:51726188-51726207	Msa0211030:intron	40.0%
	TTAGGTACTCATGCTGCTAA+AGG	-	chr2_1:51726226-51726245	Msa0211030:intron	40.0%
	CCTGAATAATGCGACGTAAT+GGG	+	chr2_1:51726261-51726280	None:intergenic	40.0%
	ACCTGAATAATGCGACGTAA+TGG	+	chr2_1:51726262-51726281	None:intergenic	40.0%
!	TGTTTGGTTTCTTGGTTGGT+GGG	-	chr2_1:51727005-51727024	Msa0211030:intron	40.0%
	ACGTAAGATTGACAATCACG+TGG	+	chr2_1:51727244-51727263	None:intergenic	40.0%
!!	GACAATAATGTAGTGGACGA+AGG	+	chr2_1:51727410-51727429	None:intergenic	40.0%
	AAGCAGGGACAATAATGTAG+TGG	+	chr2_1:51727417-51727436	None:intergenic	40.0%
	AGGGAATGAAACGTTATCCT+AGG	+	chr2_1:51727502-51727521	None:intergenic	40.0%
!!	AATAAGCTTGCTGTGCTCTT+TGG	+	chr2_1:51727942-51727961	None:intergenic	40.0%
	GACTGTGATAGAAAGAAGTG+AGG	-	chr2_1:51728040-51728059	Msa0211030:intron	40.0%
!	AATGTGCCTTATTACCTCTG+CGG	+	chr2_1:51728069-51728088	None:intergenic	40.0%
!!!	GGCTACTTTTGCCAAAAAAG+TGG	-	chr2_1:51728095-51728114	Msa0211030:intron	40.0%
	GTGAGTCTTCTAGGTATATG+AGG	+	chr2_1:51728124-51728143	None:intergenic	40.0%
	TAGTGAGATGTGAGTCTTCT+AGG	+	chr2_1:51728133-51728152	None:intergenic	40.0%
	TAGAAGACTCACATCTCACT+AGG	-	chr2_1:51728132-51728151	Msa0211030:intron	40.0%
	AGAAGACTCACATCTCACTA+GGG	-	chr2_1:51728133-51728152	Msa0211030:intron	40.0%
	ACTGCATCACTTTCTTGTGT+TGG	+	chr2_1:51728161-51728180	None:intergenic	40.0%
	GTTTATTGGGAAACCACCAT+AGG	-	chr2_1:51728179-51728198	Msa0211030:intron	40.0%
	ATCACCAATGCTACTACCTA+TGG	+	chr2_1:51728198-51728217	None:intergenic	40.0%
	GGTGATTATAGAGACGTTGA+TGG	-	chr2_1:51728212-51728231	Msa0211030:intron	40.0%
	TCTTGATTGCACATCAAGGA+CGG	-	chr2_1:51728237-51728256	Msa0211030:intron	40.0%
	AGGAGGAATGAAATGATCCA+TGG	+	chr2_1:51728372-51728391	None:intergenic	40.0%
	TTGTCAAGAATATCAGCAGG+AGG	+	chr2_1:51728389-51728408	None:intergenic	40.0%
	GTGTATTGGGAGATCAACAT+AGG	-	chr2_1:51728442-51728461	Msa0211030:CDS	40.0%
	TGACAAAATCAAGAGGGGAA+TGG	-	chr2_1:51728640-51728659	Msa0211030:five_prime_UTR	40.0%
	TGATCTTGAAGCCATTCGTG+TGG	+	chr2_1:51725231-51725250	None:intergenic	45.0%
	TCTTCTTGCATTTCAGGGTC+AGG	+	chr2_1:51725308-51725327	None:intergenic	45.0%
	GACCCTGAAATGCAAGAAGA+AGG	-	chr2_1:51725308-51725327	Msa0211030:three_prime_UTR	45.0%
	CCCCTTCTTCTTGCATTTCA+GGG	+	chr2_1:51725313-51725332	None:intergenic	45.0%
	CCCTGAAATGCAAGAAGAAG+GGG	-	chr2_1:51725310-51725329	Msa0211030:three_prime_UTR	45.0%
	CCTGAAATGCAAGAAGAAGG+GGG	-	chr2_1:51725311-51725330	Msa0211030:three_prime_UTR	45.0%
	GGGGCAAAAAAGAAAGCTCT+TGG	-	chr2_1:51725330-51725349	Msa0211030:three_prime_UTR	45.0%
	CTTGGGAAGCTATAAGGGAA+TGG	-	chr2_1:51725348-51725367	Msa0211030:three_prime_UTR	45.0%
	GCTCAGAAAATTTCCCCTAG+TGG	-	chr2_1:51725377-51725396	Msa0211030:exon	45.0%
	TCTGAAGGAATAGGAGCCAA+GGG	+	chr2_1:51725485-51725504	None:intergenic	45.0%
	ATCTGAAGGAATAGGAGCCA+AGG	+	chr2_1:51725486-51725505	None:intergenic	45.0%
	TTCAAAATCGTGGTGGTTCG+TGG	+	chr2_1:51725757-51725776	None:intergenic	45.0%
	AAACATGTATGCAACTGGGG+TGG	-	chr2_1:51726009-51726028	Msa0211030:intron	45.0%
	GTACTTTGGCAAATGTTGCC+AGG	-	chr2_1:51726118-51726137	Msa0211030:intron	45.0%
	AGCATGAGTACCTAACCAAG+GGG	+	chr2_1:51726221-51726240	None:intergenic	45.0%
	CAGCATGAGTACCTAACCAA+GGG	+	chr2_1:51726222-51726241	None:intergenic	45.0%
	CCCATTACGTCGCATTATTC+AGG	-	chr2_1:51726258-51726277	Msa0211030:intron	45.0%
!	GTGTTTGGTTTCTTGGTTGG+TGG	-	chr2_1:51727004-51727023	Msa0211030:intron	45.0%
!	TTGGGATTGCTGCTTATTGC+AGG	-	chr2_1:51727303-51727322	Msa0211030:intron	45.0%
!	TGGGATTGCTGCTTATTGCA+GGG	-	chr2_1:51727304-51727323	Msa0211030:intron	45.0%
!	CGTTGATTGCTTCGTGCTAA+AGG	-	chr2_1:51728001-51728020	Msa0211030:intron	45.0%
!	ATGTGCCTTATTACCTCTGC+GGG	+	chr2_1:51728068-51728087	None:intergenic	45.0%
	ACCACCATAGGTAGTAGCAT+TGG	-	chr2_1:51728191-51728210	Msa0211030:intron	45.0%
	ACCAATGCTACTACCTATGG+TGG	+	chr2_1:51728195-51728214	None:intergenic	45.0%
	GGGATCTTGATTGCACATCA+AGG	-	chr2_1:51728233-51728252	Msa0211030:intron	45.0%
	GAACCAGGATGGAAGAGATT+TGG	-	chr2_1:51728309-51728328	Msa0211030:CDS	45.0%
	TAATGTACCTGGACTCTCCA+TGG	-	chr2_1:51728352-51728371	Msa0211030:CDS	45.0%
	AAATGATCCATGGAGAGTCC+AGG	+	chr2_1:51728362-51728381	None:intergenic	45.0%
!!	CATTAAAAAAAAAATAATAT+AGG	+	chr2_1:51725836-51725855	None:intergenic	5.0%
!!	TGTAATTTAAAAAAAAAAAA+TGG	+	chr2_1:51727535-51727554	None:intergenic	5.0%
	TCTTGAAGCCATTCGTGTGG+AGG	+	chr2_1:51725228-51725247	None:intergenic	50.0%
	TGGCTTCAAGATCAGCACGT+TGG	-	chr2_1:51725237-51725256	Msa0211030:three_prime_UTR	50.0%
	CCCCCTTCTTCTTGCATTTC+AGG	+	chr2_1:51725314-51725333	None:intergenic	50.0%
!	TGGTGTCTTAGGTTGTCCCT+TGG	-	chr2_1:51725466-51725485	Msa0211030:CDS	50.0%
	GGCAAATGTTGCCAGGAATG+TGG	-	chr2_1:51726125-51726144	Msa0211030:intron	50.0%
	GGTGGCCATAAGATTGTTGC+TGG	-	chr2_1:51726166-51726185	Msa0211030:intron	50.0%
	TGGAGACATACCCCTTGGTT+AGG	-	chr2_1:51726208-51726227	Msa0211030:intron	50.0%
	GCAGCATGAGTACCTAACCA+AGG	+	chr2_1:51726223-51726242	None:intergenic	50.0%
!!	TTGGTTTCTTGGTTGGTGGG+TGG	-	chr2_1:51727008-51727027	Msa0211030:intron	50.0%
	TGCTTATTGCAGGGTAGCCT+AGG	-	chr2_1:51727313-51727332	Msa0211030:intron	50.0%
	TGCAGGGTAGCCTAGGTATT+AGG	-	chr2_1:51727320-51727339	Msa0211030:intron	50.0%
	GCAGGGTAGCCTAGGTATTA+GGG	-	chr2_1:51727321-51727340	Msa0211030:intron	50.0%
!!	TCAGGGGTACCCTAATACCT+AGG	+	chr2_1:51727333-51727352	None:intergenic	50.0%
	ATCGCGACGGTGTTTCGATT+CGG	-	chr2_1:51728263-51728282	Msa0211030:CDS	50.0%
	CGATGCAGCATAGTAGAACC+AGG	-	chr2_1:51728294-51728313	Msa0211030:CDS	50.0%
	GCAGCATAGTAGAACCAGGA+TGG	-	chr2_1:51728298-51728317	Msa0211030:CDS	50.0%
	GGTCCAAATCTCTTCCATCC+TGG	+	chr2_1:51728315-51728334	None:intergenic	50.0%
	AGCACGTTGGCGTCAGAGTT+GGG	-	chr2_1:51725250-51725269	Msa0211030:three_prime_UTR	55.0%
	GGGTCAGGACCTTCCATCAA+TGG	+	chr2_1:51725293-51725312	None:intergenic	55.0%
	CTGTGTGGAGACATACCCCT+TGG	-	chr2_1:51726203-51726222	Msa0211030:intron	55.0%
!!	TGCGACGTAATGGGCGTTGT+GGG	+	chr2_1:51726252-51726271	None:intergenic	55.0%
!!	ATGCGACGTAATGGGCGTTG+TGG	+	chr2_1:51726253-51726272	None:intergenic	55.0%
	AGGGACCCGCAGAGGTAATA+AGG	-	chr2_1:51728060-51728079	Msa0211030:intron	55.0%
	GAATCGAAACACCGTCGCGA+TGG	+	chr2_1:51728264-51728283	None:intergenic	55.0%
	CAGCACGTTGGCGTCAGAGT+TGG	-	chr2_1:51725249-51725268	Msa0211030:three_prime_UTR	60.0%
	AAGAAGTGAGGGACCCGCAG+AGG	-	chr2_1:51728052-51728071	Msa0211030:intron	60.0%
	TCAAGGACGGTCCATCGCGA+CGG	-	chr2_1:51728250-51728269	Msa0211030:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	51725128	51728681	51725128	ID=Msa0211030;Name=Msa0211030
chr2_1	mRNA	51725128	51728681	51725128	ID=Msa0211030-mRNA-1;Parent=Msa0211030;Name=Msa0211030-mRNA-1;_AED=0.00;_eAED=0.00;_QI=108\|1\|1\|1\|1\|1\|3\|257\|387
chr2_1	exon	51725128	51725879	51725128	ID=Msa0211030-mRNA-1:exon:14381;Parent=Msa0211030-mRNA-1
chr2_1	exon	51727530	51727877	51727530	ID=Msa0211030-mRNA-1:exon:14380;Parent=Msa0211030-mRNA-1
chr2_1	exon	51728253	51728681	51728253	ID=Msa0211030-mRNA-1:exon:14379;Parent=Msa0211030-mRNA-1
chr2_1	five_prime_UTR	51728574	51728681	51728574	ID=Msa0211030-mRNA-1:five_prime_utr;Parent=Msa0211030-mRNA-1
chr2_1	CDS	51728253	51728573	51728253	ID=Msa0211030-mRNA-1:cds;Parent=Msa0211030-mRNA-1
chr2_1	CDS	51727530	51727877	51727530	ID=Msa0211030-mRNA-1:cds;Parent=Msa0211030-mRNA-1
chr2_1	CDS	51725385	51725879	51725385	ID=Msa0211030-mRNA-1:cds;Parent=Msa0211030-mRNA-1
chr2_1	three_prime_UTR	51725128	51725384	51725128	ID=Msa0211030-mRNA-1:three_prime_utr;Parent=Msa0211030-mRNA-1

Gene Sequence

>Msa0211030

ATGGCTTCAAGATCAGCACGTTGGCGTCAGAGTTGGGCACCACAGCCATTAACACCATTGATGGAAGGTCCTGACCCTGAAATGCAAGAAGAAGGGGGCAAAAAAGAAAGCTCTTGGGAAGCTATAAGGGAATGGTTCAAAGCTCAGAAAATTTCCCCTAGTGGAAACATTTCATCACAATCATTTTATGGAACCATTCATGCTAAGACACAAGATTTAAGGTTATTGCTTGGTGTCTTAGGTTGTCCCTTGGCTCCTATTCCTTCAGATCATGATCCTGCTTTAAGTATTCATCACAATCATATCAAAGATACCCCATTTGAAACATCAACAGCCAAGTACATCATACAACAATATTTAGCTGCAACAGGATGTTTGAAACAACAAAAGGAGAATAAAAACATGTATGCAACTGGGGTGGTGAAGATGATTTGTTGTGAAACGGAGATATCATCAGGGAAGAATGTAAAGTGTTTGGGGACAAGAAGTAGTGAAAATGGTTGCTTTGTACTTTGGCAAATGTTGCCAGGAATGTGGTCACTTGAATTGGTTGTTGGTGGCCATAAGATTGTTGCTGGTAGTAATGGTAAGACTGTGTGGAGACATACCCCTTGGTTAGGTACTCATGCTGCTAAAGGCCCACAACGCCCATTACGTCGCATTATTCAGGGTTTGGATCCAAAGAGCACAGCAAGCTTATTCACAAATGCACAATGCTTAGGTGAAAATCGAATCGGCAACGTTGATTGCTTCGTGCTAAAGGTATGTGCAGATCGTGAGACTGTGATAGAAAGAAGTGAGGGACCCGCAGAGGTAATAAGGCACATTCTATATGGCTACTTTTGCCAAAAAAGTGGACTCCTCATATACCTAGAAGACTCACATCTCACTAGGGTTCCAACACAAGAAAGTGATGCAGTTTATTGGGAAACCACCATAGGTAGTAGCATTGGTGATTATAGAGACGTTGATGGGATCTTGATTGCACATCAAGGACGGTCCATCGCGACGGTGTTTCGATTCGGAGAACTCTCGATGCAGCATAGTAGAACCAGGATGGAAGAGATTTGGACCATTGATGATGTTATGTTTAATGTACCTGGACTCTCCATGGATCATTTCATTCCTCCTGCTGATATTCTTGACAATATAAATTCTCCATGA

Protein sequence

>Msa0211030

MASRSARWRQSWAPQPLTPLMEGPDPEMQEEGGKKESSWEAIREWFKAQKISPSGNISSQSFYGTIHAKTQDLRLLLGVLGCPLAPIPSDHDPALSIHHNHIKDTPFETSTAKYIIQQYLAATGCLKQQKENKNMYATGVVKMICCETEISSGKNVKCLGTRSSENGCFVLWQMLPGMWSLELVVGGHKIVAGSNGKTVWRHTPWLGTHAAKGPQRPLRRIIQGLDPKSTASLFTNAQCLGENRIGNVDCFVLKVCADRETVIERSEGPAEVIRHILYGYFCQKSGLLIYLEDSHLTRVPTQESDAVYWETTIGSSIGDYRDVDGILIAHQGRSIATVFRFGELSMQHSRTRMEEIWTIDDVMFNVPGLSMDHFIPPADILDNINSP*