Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0317750 | XP_003594309.1 | 98.795 | 166 | 2 | 0 | 1 | 166 | 1 | 166 | 1.91e-113 | 329 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0317750 | sp|Q9FIK8|CAR11_ARATH | 69.880 | 166 | 50 | 0 | 1 | 166 | 1 | 166 | 2.69e-83 | 245 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0037570 | Msa0317750 | -0.809649 | 1.704412e-50 | -8.615850e-47 |
| Msa0068740 | Msa0317750 | -0.819348 | 1.217798e-52 | -8.615850e-47 |
| Msa0075880 | Msa0317750 | 0.822135 | 2.785553e-53 | -8.615850e-47 |
| Msa0078900 | Msa0317750 | 0.811481 | 6.852455e-51 | -8.615850e-47 |
| Msa0100090 | Msa0317750 | 0.816959 | 4.225376e-52 | -8.615850e-47 |
| Msa0114060 | Msa0317750 | 0.800671 | 1.291628e-48 | -8.615850e-47 |
| Msa0136070 | Msa0317750 | 0.810117 | 1.352113e-50 | -8.615850e-47 |
| Msa0150840 | Msa0317750 | 0.817837 | 2.680624e-52 | -8.615850e-47 |
| Msa0171540 | Msa0317750 | 0.800897 | 1.161868e-48 | -8.615850e-47 |
| Msa0173990 | Msa0317750 | 0.804816 | 1.801744e-49 | -8.615850e-47 |
| Msa0180500 | Msa0317750 | 0.810960 | 8.886315e-51 | -8.615850e-47 |
| Msa0197660 | Msa0317750 | 0.838737 | 2.406783e-57 | -8.615850e-47 |
| Msa0208090 | Msa0317750 | 0.801128 | 1.042002e-48 | -8.615850e-47 |
| Msa0239800 | Msa0317750 | 0.826091 | 3.281151e-54 | -8.615850e-47 |
| Msa0260680 | Msa0317750 | 0.812674 | 3.764284e-51 | -8.615850e-47 |
| Msa0277820 | Msa0317750 | 0.809424 | 1.905079e-50 | -8.615850e-47 |
| Msa0280090 | Msa0317750 | 0.808031 | 3.782327e-50 | -8.615850e-47 |
| Msa0285450 | Msa0317750 | 0.819101 | 1.385624e-52 | -8.615850e-47 |
| Msa0306210 | Msa0317750 | -0.802338 | 5.881398e-49 | -8.615850e-47 |
| Msa0317510 | Msa0317750 | 0.828426 | 9.049591e-55 | -8.615850e-47 |
| Msa0317720 | Msa0317750 | 0.968584 | 6.924171e-129 | -8.615850e-47 |
| Msa0317750 | Msa0319170 | 0.805513 | 1.287539e-49 | -8.615850e-47 |
| Msa0317750 | Msa0340450 | 0.835706 | 1.435634e-56 | -8.615850e-47 |
| Msa0317750 | Msa0383800 | 0.812362 | 4.404571e-51 | -8.615850e-47 |
| Msa0317750 | Msa0390400 | -0.809072 | 2.267053e-50 | -8.615850e-47 |
| Msa0317750 | Msa0412840 | 0.805863 | 1.086894e-49 | -8.615850e-47 |
| Msa0317750 | Msa0431060 | 0.807916 | 4.001669e-50 | -8.615850e-47 |
| Msa0317750 | Msa0434180 | 0.825553 | 4.401889e-54 | -8.615850e-47 |
| Msa0317750 | Msa0435690 | 0.815965 | 7.054835e-52 | -8.615850e-47 |
| Msa0317750 | Msa0436760 | 0.820976 | 5.159099e-53 | -8.615850e-47 |
| Msa0317750 | Msa0443920 | 0.801377 | 9.265546e-49 | -8.615850e-47 |
| Msa0317750 | Msa0453330 | 0.817119 | 3.890500e-52 | -8.615850e-47 |
| Msa0317750 | Msa0479340 | 0.800870 | 1.176318e-48 | -8.615850e-47 |
| Msa0317750 | Msa0479660 | 0.804036 | 2.619128e-49 | -8.615850e-47 |
| Msa0317750 | Msa0529890 | 0.808901 | 2.466000e-50 | -8.615850e-47 |
| Msa0317750 | Msa0530400 | 0.813585 | 2.375634e-51 | -8.615850e-47 |
| Msa0317750 | Msa0548920 | 0.804427 | 2.171204e-49 | -8.615850e-47 |
| Msa0317750 | Msa0558690 | 0.800795 | 1.218598e-48 | -8.615850e-47 |
| Msa0317750 | Msa0625720 | 0.803847 | 2.867639e-49 | -8.615850e-47 |
| Msa0317750 | Msa0632740 | 0.821796 | 3.337737e-53 | -8.615850e-47 |
| Msa0317750 | Msa0657610 | 0.814539 | 1.463521e-51 | -8.615850e-47 |
| Msa0317750 | Msa0661190 | -0.816357 | 5.765939e-52 | -8.615850e-47 |
| Msa0317750 | Msa0673460 | 0.811961 | 5.386778e-51 | -8.615850e-47 |
| Msa0317750 | Msa0679290 | -0.814721 | 1.333415e-51 | -8.615850e-47 |
| Msa0317750 | Msa0715700 | 0.801439 | 8.997790e-49 | -8.615850e-47 |
| Msa0317750 | Msa0724210 | 0.801876 | 7.320001e-49 | -8.615850e-47 |
| Msa0317750 | Msa0731940 | 0.831536 | 1.578699e-55 | -8.615850e-47 |
| Msa0317750 | Msa0743720 | 0.807196 | 5.689535e-50 | -8.615850e-47 |
| Msa0317750 | Msa0744170 | -0.814193 | 1.745106e-51 | -8.615850e-47 |
| Msa0317750 | Msa0773840 | 0.804495 | 2.102169e-49 | -8.615850e-47 |
| Msa0317750 | Msa0810220 | 0.812610 | 3.888295e-51 | -8.615850e-47 |
| Msa0317750 | Msa0820850 | 0.823921 | 1.067802e-53 | -8.615850e-47 |
| Msa0317750 | Msa0835800 | 0.803769 | 2.975854e-49 | -8.615850e-47 |
| Msa0317750 | Msa0857640 | 0.816965 | 4.212934e-52 | -8.615850e-47 |
| Msa0317750 | Msa0861130 | 0.816263 | 6.051679e-52 | -8.615850e-47 |
| Msa0317750 | Msa0871270 | 0.807522 | 4.851091e-50 | -8.615850e-47 |
| Msa0317750 | Msa0936300 | 0.809025 | 2.319933e-50 | -8.615850e-47 |
| Msa0317750 | Msa0938220 | 0.808869 | 2.505873e-50 | -8.615850e-47 |
| Msa0317750 | Msa0942550 | -0.809282 | 2.044194e-50 | -8.615850e-47 |
| Msa0317750 | Msa0993600 | 0.800154 | 1.646309e-48 | -8.615850e-47 |
| Msa0317750 | Msa0998690 | 0.806267 | 8.937129e-50 | -8.615850e-47 |
| Msa0317750 | Msa1003620 | 0.814075 | 1.853452e-51 | -8.615850e-47 |
| Msa0317750 | Msa1014540 | 0.834755 | 2.495444e-56 | -8.615850e-47 |
| Msa0317750 | Msa1023120 | 0.807459 | 5.002975e-50 | -8.615850e-47 |
| Msa0317750 | Msa1033410 | -0.821831 | 3.275839e-53 | -8.615850e-47 |
| Msa0317750 | Msa1036710 | -0.807024 | 6.186230e-50 | -8.615850e-47 |
| Msa0317750 | Msa1046060 | 0.804470 | 2.127060e-49 | -8.615850e-47 |
| Msa0317750 | Msa1046460 | 0.818597 | 1.804386e-52 | -8.615850e-47 |
| Msa0317750 | Msa1052330 | 0.844406 | 7.712244e-59 | -8.615850e-47 |
| Msa0317750 | Msa1073070 | -0.813927 | 1.998004e-51 | -8.615850e-47 |
| Msa0317750 | Msa1082500 | 0.802867 | 4.576647e-49 | -8.615850e-47 |
| Msa0317750 | Msa1131220 | 0.805020 | 1.632592e-49 | -8.615850e-47 |
| Msa0317750 | Msa1154280 | 0.816175 | 6.331572e-52 | -8.615850e-47 |
| Msa0317750 | Msa1172400 | 0.815000 | 1.156785e-51 | -8.615850e-47 |
| Msa0317750 | Msa1210910 | 0.814494 | 1.497240e-51 | -8.615850e-47 |
| Msa0317750 | Msa1211140 | -0.824062 | 9.892398e-54 | -8.615850e-47 |
| Msa0317750 | Msa1238930 | 0.830773 | 2.431177e-55 | -8.615850e-47 |
| Msa0317750 | Msa1244250 | 0.823069 | 1.689074e-53 | -8.615850e-47 |
| Msa0317750 | Msa1249490 | 0.818623 | 1.779284e-52 | -8.615850e-47 |
| Msa0317750 | Msa1259300 | 0.813502 | 2.477715e-51 | -8.615850e-47 |
| Msa0317750 | Msa1277660 | 0.819292 | 1.253826e-52 | -8.615850e-47 |
| Msa0317750 | Msa1282580 | -0.800402 | 1.465806e-48 | -8.615850e-47 |
| Msa0317750 | Msa1284680 | 0.809833 | 1.555969e-50 | -8.615850e-47 |
| Msa0317750 | Msa1299290 | -0.820916 | 5.327070e-53 | -8.615850e-47 |
| Msa0317750 | Msa1305190 | 0.805587 | 1.242473e-49 | -8.615850e-47 |
| Msa0317750 | Msa1305340 | 0.817290 | 3.560382e-52 | -8.615850e-47 |
| Msa0317750 | Msa1320410 | 0.820623 | 6.219339e-53 | -8.615850e-47 |
| Msa0317750 | Msa1320620 | 0.801454 | 8.935206e-49 | -8.615850e-47 |
| Msa0317750 | Msa1332040 | -0.801421 | 9.074313e-49 | -8.615850e-47 |
| Msa0317750 | Msa1344130 | 0.811943 | 5.436053e-51 | -8.615850e-47 |
| Msa0317750 | Msa1358670 | 0.819439 | 1.160651e-52 | -8.615850e-47 |
| Msa0317750 | Msa1374470 | 0.806246 | 9.028683e-50 | -8.615850e-47 |
| Msa0317750 | Msa1395660 | 0.802989 | 4.316704e-49 | -8.615850e-47 |
| Msa0317750 | Msa1403340 | 0.815791 | 7.712196e-52 | -8.615850e-47 |
| Msa0317750 | Msa1412640 | 0.801716 | 7.895526e-49 | -8.615850e-47 |
| Msa0317750 | Msa1430680 | 0.802582 | 5.240124e-49 | -8.615850e-47 |
| Msa0317750 | Msa1449340 | -0.803975 | 2.697481e-49 | -8.615850e-47 |
| Msa0317750 | Msa1455490 | 0.803847 | 2.867501e-49 | -8.615850e-47 |
| Msa0317750 | Msa1466540 | 0.822585 | 2.190131e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0317750 | MtrunA17_Chr2g0290631 | 98.795 | 166 | 2 | 0 | 1 | 166 | 1 | 166 | 1.76e-117 | 329 |
| Msa0317750 | MtrunA17_Chr8g0380381 | 47.239 | 163 | 73 | 2 | 1 | 157 | 85 | 240 | 8.18e-43 | 142 |
| Msa0317750 | MtrunA17_Chr3g0111361 | 43.949 | 157 | 86 | 1 | 1 | 157 | 1 | 155 | 1.99e-39 | 131 |
| Msa0317750 | MtrunA17_Chr2g0280981 | 43.137 | 153 | 85 | 1 | 5 | 157 | 4 | 154 | 3.23e-39 | 130 |
| Msa0317750 | MtrunA17_Chr2g0292461 | 45.860 | 157 | 84 | 1 | 1 | 157 | 168 | 323 | 6.36e-38 | 132 |
| Msa0317750 | MtrunA17_Chr8g0393081 | 42.675 | 157 | 88 | 1 | 1 | 157 | 17 | 171 | 7.47e-37 | 125 |
| Msa0317750 | MtrunA17_Chr6g0478401 | 40.764 | 157 | 91 | 2 | 1 | 157 | 13 | 167 | 9.49e-37 | 125 |
| Msa0317750 | MtrunA17_Chr8g0352751 | 43.949 | 157 | 87 | 1 | 1 | 157 | 1 | 156 | 3.26e-35 | 120 |
| Msa0317750 | MtrunA17_Chr3g0083541 | 46.497 | 157 | 83 | 1 | 1 | 157 | 239 | 394 | 6.24e-35 | 125 |
| Msa0317750 | MtrunA17_Chr4g0043861 | 39.474 | 152 | 90 | 1 | 1 | 152 | 1 | 150 | 2.03e-30 | 108 |
| Msa0317750 | MtrunA17_Chr1g0160811 | 42.953 | 149 | 82 | 2 | 5 | 152 | 6 | 152 | 2.10e-30 | 108 |
| Msa0317750 | MtrunA17_Chr8g0350031 | 36.458 | 96 | 59 | 2 | 6 | 99 | 4 | 99 | 5.47e-12 | 60.1 |
| Msa0317750 | MtrunA17_Chr6g0484401 | 38.053 | 113 | 61 | 5 | 7 | 113 | 484 | 593 | 2.13e-11 | 61.2 |
| Msa0317750 | MtrunA17_Chr8g0350021 | 36.264 | 91 | 56 | 2 | 6 | 94 | 4 | 94 | 4.06e-11 | 57.8 |
| Msa0317750 | MtrunA17_Chr1g0168851 | 35.556 | 90 | 56 | 2 | 6 | 93 | 127 | 216 | 4.19e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0317750 | AT5G47710.1 | 69.880 | 166 | 50 | 0 | 1 | 166 | 1 | 166 | 2.74e-84 | 245 |
| Msa0317750 | AT5G47710.2 | 69.880 | 166 | 50 | 0 | 1 | 166 | 1 | 166 | 2.74e-84 | 245 |
| Msa0317750 | AT5G47710.3 | 69.880 | 166 | 50 | 0 | 1 | 166 | 1 | 166 | 2.74e-84 | 245 |
| Msa0317750 | AT5G47710.4 | 69.880 | 166 | 50 | 0 | 1 | 166 | 1 | 166 | 2.74e-84 | 245 |
| Msa0317750 | AT1G70790.3 | 46.154 | 156 | 82 | 2 | 5 | 159 | 6 | 160 | 7.01e-40 | 133 |
| Msa0317750 | AT1G70790.2 | 46.154 | 156 | 82 | 2 | 5 | 159 | 6 | 160 | 7.01e-40 | 133 |
| Msa0317750 | AT1G70790.1 | 46.154 | 156 | 82 | 2 | 5 | 159 | 6 | 160 | 7.01e-40 | 133 |
| Msa0317750 | AT1G73580.1 | 44.586 | 157 | 85 | 1 | 1 | 157 | 4 | 158 | 8.71e-39 | 130 |
| Msa0317750 | AT2G01540.1 | 44.156 | 154 | 84 | 2 | 5 | 157 | 6 | 158 | 1.09e-37 | 127 |
| Msa0317750 | AT4G05330.2 | 45.223 | 157 | 85 | 1 | 1 | 157 | 140 | 295 | 1.10e-37 | 131 |
| Msa0317750 | AT4G05330.3 | 45.223 | 157 | 85 | 1 | 1 | 157 | 175 | 330 | 4.59e-37 | 130 |
| Msa0317750 | AT4G05330.1 | 45.223 | 157 | 85 | 1 | 1 | 157 | 175 | 330 | 4.59e-37 | 130 |
| Msa0317750 | AT3G17980.1 | 42.038 | 157 | 89 | 2 | 1 | 157 | 13 | 167 | 5.26e-37 | 125 |
| Msa0317750 | AT5G37740.1 | 41.139 | 158 | 92 | 1 | 1 | 157 | 1 | 158 | 6.57e-37 | 125 |
| Msa0317750 | AT4G21160.2 | 43.949 | 157 | 87 | 1 | 1 | 157 | 176 | 331 | 6.57e-37 | 130 |
| Msa0317750 | AT4G21160.3 | 43.949 | 157 | 87 | 1 | 1 | 157 | 176 | 331 | 6.57e-37 | 130 |
| Msa0317750 | AT4G21160.1 | 43.949 | 157 | 87 | 1 | 1 | 157 | 176 | 331 | 6.57e-37 | 130 |
| Msa0317750 | AT4G21160.4 | 43.949 | 157 | 87 | 1 | 1 | 157 | 176 | 331 | 6.57e-37 | 130 |
| Msa0317750 | AT1G66360.1 | 39.490 | 157 | 95 | 0 | 1 | 157 | 1 | 157 | 2.14e-36 | 124 |
| Msa0317750 | AT1G70810.1 | 41.401 | 157 | 90 | 2 | 1 | 157 | 1 | 155 | 6.43e-36 | 122 |
| Msa0317750 | AT3G07940.1 | 45.860 | 157 | 84 | 1 | 1 | 157 | 224 | 379 | 4.47e-35 | 126 |
| Msa0317750 | AT1G48590.4 | 40.127 | 157 | 92 | 2 | 1 | 157 | 6 | 160 | 2.58e-34 | 119 |
| Msa0317750 | AT1G48590.1 | 40.127 | 157 | 92 | 2 | 1 | 157 | 6 | 160 | 2.58e-34 | 119 |
| Msa0317750 | AT1G23140.1 | 37.576 | 165 | 101 | 2 | 1 | 165 | 1 | 163 | 4.67e-34 | 118 |
| Msa0317750 | AT1G48590.2 | 40.127 | 157 | 92 | 2 | 1 | 157 | 37 | 191 | 5.11e-34 | 119 |
| Msa0317750 | AT5G37740.2 | 38.690 | 168 | 92 | 2 | 1 | 157 | 1 | 168 | 1.94e-33 | 117 |
| Msa0317750 | AT1G70800.1 | 36.943 | 157 | 97 | 1 | 1 | 157 | 10 | 164 | 1.71e-31 | 112 |
| Msa0317750 | AT1G70790.4 | 42.975 | 121 | 67 | 2 | 40 | 159 | 4 | 123 | 7.11e-27 | 99.4 |
| Msa0317750 | AT1G48590.3 | 32.484 | 157 | 72 | 3 | 1 | 157 | 37 | 159 | 8.79e-20 | 81.6 |
Find 60 sgRNAs with CRISPR-Local
Find 121 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATGAAAAGTTGTGATTTA+TGG | 0.068719 | 2_4:+15706250 | Msa0317750:three_prime_UTR |
| CCTTATGTTGTTCTCAAATT+AGG | 0.114099 | 2_4:+15705023 | Msa0317750:CDS |
| TTTGAAAGCTGATGACAAAA+TGG | 0.206431 | 2_4:+15705810 | Msa0317750:CDS |
| TGATTGAGTATGAGAATTTA+TGG | 0.234568 | 2_4:-15704068 | None:intergenic |
| AGTTCTAATTGCATAGGTTT+AGG | 0.235916 | 2_4:+15706404 | Msa0317750:three_prime_UTR |
| GTTCTAATTGCATAGGTTTA+GGG | 0.287937 | 2_4:+15706405 | Msa0317750:three_prime_UTR |
| ATTATAATGAGTGAACAATT+AGG | 0.300153 | 2_4:+15704936 | Msa0317750:exon |
| ACCCTTGGGAGTTCTCAATT+TGG | 0.326299 | 2_4:+15705416 | Msa0317750:CDS |
| CAATTCTCTCTTATCTAATC+AGG | 0.328553 | 2_4:-15706214 | None:intergenic |
| CTTCCGGTGAACAAACATTA+AGG | 0.352544 | 2_4:+15705899 | Msa0317750:CDS |
| GTTGTGATTTATGGCTTCTC+TGG | 0.365327 | 2_4:+15706259 | Msa0317750:three_prime_UTR |
| TGTTCTCAAATTAGGAAATC+AGG | 0.369709 | 2_4:+15705031 | Msa0317750:CDS |
| TGATATTCTTCATTATTCAT+TGG | 0.382002 | 2_4:+15704110 | Msa0317750:five_prime_UTR |
| CCTAATTTGAGAACAACATA+AGG | 0.397853 | 2_4:-15705023 | None:intergenic |
| GCGCGGTTGTGCAGAATGTT+TGG | 0.408048 | 2_4:+15705983 | Msa0317750:CDS |
| ATAGCTGTTTGAATCCGGTT+TGG | 0.419279 | 2_4:+15705364 | Msa0317750:CDS |
| AAGGGAAAAGGTTGGTGATC+CGG | 0.421394 | 2_4:+15704982 | Msa0317750:CDS |
| TTGAAAGCTGATGACAAAAT+GGG | 0.427442 | 2_4:+15705811 | Msa0317750:CDS |
| AGCAGTATCAATTGTGTTAA+CGG | 0.427721 | 2_4:+15705961 | Msa0317750:CDS |
| TAAATTGCATTATAATATGC+AGG | 0.429332 | 2_4:-15706188 | None:intergenic |
| CATTAATAGCTGTTTGAATC+CGG | 0.434724 | 2_4:+15705359 | Msa0317750:CDS |
| TTATAATGAGTGAACAATTA+GGG | 0.440831 | 2_4:+15704937 | Msa0317750:exon |
| TTTCTCTTTGACAGAACCCT+TGG | 0.442241 | 2_4:+15705401 | Msa0317750:CDS |
| TTCAGCTCTTCATTCCAAAC+CGG | 0.443481 | 2_4:-15705378 | None:intergenic |
| TTGTGCAGAATGTTTGGTTA+AGG | 0.448350 | 2_4:+15705989 | Msa0317750:CDS |
| TGAGAATTTATGGAAGGGAT+TGG | 0.458155 | 2_4:-15704058 | None:intergenic |
| AATCGAGCTGCAGAGACTAA+TGG | 0.466284 | 2_4:-15705856 | None:intergenic |
| TTCTCTTTGACAGAACCCTT+GGG | 0.470160 | 2_4:+15705402 | Msa0317750:CDS |
| AGGGAAAAGGTTGGTGATCC+GGG | 0.470972 | 2_4:+15704983 | Msa0317750:CDS |
| CAAACAGCTATTAATGACCT+TGG | 0.491295 | 2_4:-15705352 | None:intergenic |
| GAGTGTCAATTCTATCTCTC+CGG | 0.491542 | 2_4:-15706028 | None:intergenic |
| CTGCAGAGACTAATGGTTGA+AGG | 0.508368 | 2_4:-15705849 | None:intergenic |
| TCATAGTTGTGCAAGGGAAA+AGG | 0.522477 | 2_4:+15704970 | Msa0317750:CDS |
| CTTCCTTAATGTTTGTTCAC+CGG | 0.524733 | 2_4:-15705902 | None:intergenic |
| GATCACTGGTCTTGAAATCC+CGG | 0.525136 | 2_4:-15705001 | None:intergenic |
| GAATTTATGGAAGGGATTGG+TGG | 0.528275 | 2_4:-15704055 | None:intergenic |
| TGAGTATGAGAATTTATGGA+AGG | 0.534908 | 2_4:-15704064 | None:intergenic |
| CTATTAAGGAAGTAGAGTAA+AGG | 0.537305 | 2_4:+15706444 | Msa0317750:three_prime_UTR |
| AGGCTCCGTGAAGTAGAATC+CGG | 0.538501 | 2_4:+15706009 | Msa0317750:CDS |
| TCTCTCCGGATTCTACTTCA+CGG | 0.540740 | 2_4:-15706014 | None:intergenic |
| CTTTGACCACCTGTAGCTGC+AGG | 0.552730 | 2_4:+15704005 | Msa0317750:five_prime_UTR |
| CAATTAGAACTCATTAATCA+AGG | 0.553464 | 2_4:-15706392 | None:intergenic |
| TTAATGAGTTCTAATTGCAT+AGG | 0.556403 | 2_4:+15706398 | Msa0317750:three_prime_UTR |
| GAGAACAACATAAGGATCAC+TGG | 0.556443 | 2_4:-15705015 | None:intergenic |
| AAATTGCATTATAATATGCA+GGG | 0.557931 | 2_4:-15706187 | None:intergenic |
| TGAACAATTAGGGCAGCTAA+AGG | 0.572900 | 2_4:+15704947 | Msa0317750:CDS |
| GAGTATGAGAATTTATGGAA+GGG | 0.583980 | 2_4:-15704063 | None:intergenic |
| TTCTAATTGCATAGGTTTAG+GGG | 0.585355 | 2_4:+15706406 | Msa0317750:three_prime_UTR |
| CTACTTCAAAATAGTGCAGA+GGG | 0.588994 | 2_4:+15706070 | Msa0317750:exon |
| CTAAAGGTCATAGTTGTGCA+AGG | 0.591043 | 2_4:+15704963 | Msa0317750:CDS |
| CACCAAATTGAGAACTCCCA+AGG | 0.596444 | 2_4:-15705418 | None:intergenic |
| TTGGTGGAACGTGAAGAAGC+AGG | 0.607051 | 2_4:-15704039 | None:intergenic |
| CTGCAGCTACAGGTGGTCAA+AGG | 0.607125 | 2_4:-15704004 | None:intergenic |
| TAAAGGTCATAGTTGTGCAA+GGG | 0.629000 | 2_4:+15704964 | Msa0317750:CDS |
| CGGTGAACAAACATTAAGGA+AGG | 0.629985 | 2_4:+15705903 | Msa0317750:CDS |
| AGTTGTGCAAGGGAAAAGGT+TGG | 0.639430 | 2_4:+15704974 | Msa0317750:CDS |
| ACCAAATTGAGAACTCCCAA+GGG | 0.650902 | 2_4:-15705417 | None:intergenic |
| GCTACTTCAAAATAGTGCAG+AGG | 0.656916 | 2_4:+15706069 | Msa0317750:exon |
| GTAACAGACTGCAAAGACCA+AGG | 0.686957 | 2_4:+15705335 | Msa0317750:intron |
| TATCAATTGTGTTAACGGCG+CGG | 0.708048 | 2_4:+15705966 | Msa0317750:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTCTTTATTTTATTTAATG+TGG | + | chr2_4:15704287-15704306 | Msa0317750:intron | 10.0% |
| !!! | TTGATAATTCAATTAAATAA+TGG | - | chr2_4:15704356-15704375 | None:intergenic | 10.0% |
| !!! | ATTCAATTAAATAATGGATA+TGG | - | chr2_4:15704350-15704369 | None:intergenic | 15.0% |
| !!! | TATTATTTCATTTTCATCTT+AGG | + | chr2_4:15705065-15705084 | Msa0317750:intron | 15.0% |
| !!! | TATTTTTGTATTGACTATTA+AGG | + | chr2_4:15706430-15706449 | Msa0317750:three_prime_UTR | 15.0% |
| !! | TGATATTCTTCATTATTCAT+TGG | + | chr2_4:15704110-15704129 | Msa0317750:five_prime_UTR | 20.0% |
| !! | TTATTTAATGTGGATTTACT+TGG | + | chr2_4:15704297-15704316 | Msa0317750:intron | 20.0% |
| !!! | ATTGATATTTTCATTTGTGT+TGG | - | chr2_4:15704843-15704862 | None:intergenic | 20.0% |
| !!! | CGATTAATTATTTAACTTGT+TGG | + | chr2_4:15704908-15704927 | Msa0317750:intron | 20.0% |
| !!! | TAATTATTTAACTTGTTGGT+AGG | + | chr2_4:15704912-15704931 | Msa0317750:intron | 20.0% |
| !! | ATTATAATGAGTGAACAATT+AGG | + | chr2_4:15704936-15704955 | Msa0317750:exon | 20.0% |
| !! | TTATAATGAGTGAACAATTA+GGG | + | chr2_4:15704937-15704956 | Msa0317750:exon | 20.0% |
| !! | GAATGAATCATTTAATATTG+AGG | - | chr2_4:15705307-15705326 | None:intergenic | 20.0% |
| !! | AAATTGCATTATAATATGCA+GGG | - | chr2_4:15706190-15706209 | None:intergenic | 20.0% |
| !! | TAAATTGCATTATAATATGC+AGG | - | chr2_4:15706191-15706210 | None:intergenic | 20.0% |
| !! | AAATGAAAAGTTGTGATTTA+TGG | + | chr2_4:15706250-15706269 | Msa0317750:three_prime_UTR | 20.0% |
| ! | TGATTGAGTATGAGAATTTA+TGG | - | chr2_4:15704071-15704090 | None:intergenic | 25.0% |
| ! | TTTCTCTACTAGAATATTCA+TGG | + | chr2_4:15704539-15704558 | Msa0317750:intron | 25.0% |
| !! | TGATTTTCATGTAATTTTGC+TGG | + | chr2_4:15704600-15704619 | Msa0317750:intron | 25.0% |
| !!! | TGTTCTTTTTTGTCCTTTTT+GGG | + | chr2_4:15704654-15704673 | Msa0317750:intron | 25.0% |
| !! | ATTTATCTTTTGACCCAAAA+AGG | - | chr2_4:15704670-15704689 | None:intergenic | 25.0% |
| ! | GTTTGTTTATCTTCACATTA+AGG | + | chr2_4:15705087-15705106 | Msa0317750:intron | 25.0% |
| ! | TGGCATTGTAAATAAATACT+AGG | - | chr2_4:15705449-15705468 | None:intergenic | 25.0% |
| ! | ATTTACAATGCCATGTTTAT+TGG | + | chr2_4:15705456-15705475 | Msa0317750:intron | 25.0% |
| ! | CTCTAACACAAGAATAAATA+AGG | - | chr2_4:15705687-15705706 | None:intergenic | 25.0% |
| ! | AATGCAACTCAGTTATATAT+AGG | - | chr2_4:15705760-15705779 | None:intergenic | 25.0% |
| ! | CAATTAGAACTCATTAATCA+AGG | - | chr2_4:15706395-15706414 | None:intergenic | 25.0% |
| !! | TTAATGAGTTCTAATTGCAT+AGG | + | chr2_4:15706398-15706417 | Msa0317750:three_prime_UTR | 25.0% |
| GAGTATGAGAATTTATGGAA+GGG | - | chr2_4:15704066-15704085 | None:intergenic | 30.0% | |
| TGAGTATGAGAATTTATGGA+AGG | - | chr2_4:15704067-15704086 | None:intergenic | 30.0% | |
| TAATGTGGATTTACTTGGTT+AGG | + | chr2_4:15704302-15704321 | Msa0317750:intron | 30.0% | |
| !!! | CTGTTCTTTTTTGTCCTTTT+TGG | + | chr2_4:15704653-15704672 | Msa0317750:intron | 30.0% |
| AACCAAAAGCAGTAAACAAA+AGG | - | chr2_4:15704724-15704743 | None:intergenic | 30.0% | |
| ! | TGGTACAAATCTTTGAAAAC+AGG | - | chr2_4:15704823-15704842 | None:intergenic | 30.0% |
| TTGTTCACTCATATATTCAG+GGG | - | chr2_4:15704875-15704894 | None:intergenic | 30.0% | |
| GTTGTTCACTCATATATTCA+GGG | - | chr2_4:15704876-15704895 | None:intergenic | 30.0% | |
| AGTTGTTCACTCATATATTC+AGG | - | chr2_4:15704877-15704896 | None:intergenic | 30.0% | |
| GAATATATGAGTGAACAACT+AGG | + | chr2_4:15704877-15704896 | Msa0317750:intron | 30.0% | |
| CCTAATTTGAGAACAACATA+AGG | - | chr2_4:15705026-15705045 | None:intergenic | 30.0% | |
| CCTTATGTTGTTCTCAAATT+AGG | + | chr2_4:15705023-15705042 | Msa0317750:CDS | 30.0% | |
| TGTTCTCAAATTAGGAAATC+AGG | + | chr2_4:15705031-15705050 | Msa0317750:CDS | 30.0% | |
| CTTCACATTAAGGACATATT+TGG | + | chr2_4:15705097-15705116 | Msa0317750:intron | 30.0% | |
| ATTGCTAAAAAAACACCTGT+TGG | + | chr2_4:15705166-15705185 | Msa0317750:intron | 30.0% | |
| TGCATCTTCTAAGTTTAATG+TGG | - | chr2_4:15705244-15705263 | None:intergenic | 30.0% | |
| CATTAATAGCTGTTTGAATC+CGG | + | chr2_4:15705359-15705378 | Msa0317750:CDS | 30.0% | |
| GCAAAAAAGACCAATAAACA+TGG | - | chr2_4:15705469-15705488 | None:intergenic | 30.0% | |
| !!! | GTTTATTGGTCTTTTTTGCT+TGG | + | chr2_4:15705470-15705489 | Msa0317750:intron | 30.0% |
| GGTATCTACATGTTGTATAT+CGG | + | chr2_4:15705495-15705514 | Msa0317750:intron | 30.0% | |
| TTAATCTGTTTATCCGATCA+TGG | + | chr2_4:15705734-15705753 | Msa0317750:intron | 30.0% | |
| AGTTATATATAGGCCATGAT+CGG | - | chr2_4:15705750-15705769 | None:intergenic | 30.0% | |
| TTTGAAAGCTGATGACAAAA+TGG | + | chr2_4:15705810-15705829 | Msa0317750:CDS | 30.0% | |
| TTGAAAGCTGATGACAAAAT+GGG | + | chr2_4:15705811-15705830 | Msa0317750:CDS | 30.0% | |
| AGCAGTATCAATTGTGTTAA+CGG | + | chr2_4:15705961-15705980 | Msa0317750:CDS | 30.0% | |
| CAATTCTCTCTTATCTAATC+AGG | - | chr2_4:15706217-15706236 | None:intergenic | 30.0% | |
| ! | AGTTCTAATTGCATAGGTTT+AGG | + | chr2_4:15706404-15706423 | Msa0317750:three_prime_UTR | 30.0% |
| GTTCTAATTGCATAGGTTTA+GGG | + | chr2_4:15706405-15706424 | Msa0317750:three_prime_UTR | 30.0% | |
| TTCTAATTGCATAGGTTTAG+GGG | + | chr2_4:15706406-15706425 | Msa0317750:three_prime_UTR | 30.0% | |
| CTATTAAGGAAGTAGAGTAA+AGG | + | chr2_4:15706444-15706463 | Msa0317750:three_prime_UTR | 30.0% | |
| TGAGAATTTATGGAAGGGAT+TGG | - | chr2_4:15704061-15704080 | None:intergenic | 35.0% | |
| TAGTTCATCTCTGACATACT+TGG | + | chr2_4:15704234-15704253 | Msa0317750:intron | 35.0% | |
| !! | GTCCTTTTGTTTACTGCTTT+TGG | + | chr2_4:15704719-15704738 | Msa0317750:intron | 35.0% |
| AATCAATGCATTGCATAGAC+AGG | - | chr2_4:15704754-15704773 | None:intergenic | 35.0% | |
| !! | ATGCATTGATTCCTCTCAAA+AGG | + | chr2_4:15704763-15704782 | Msa0317750:intron | 35.0% |
| !! | TGCATTGATTCCTCTCAAAA+GGG | + | chr2_4:15704764-15704783 | Msa0317750:intron | 35.0% |
| ! | GAAACTATTTCCCTTTTGAG+AGG | - | chr2_4:15704777-15704796 | None:intergenic | 35.0% |
| ATGAATAATGTATGCCATGG+TGG | - | chr2_4:15704799-15704818 | None:intergenic | 35.0% | |
| GGAATGAATAATGTATGCCA+TGG | - | chr2_4:15704802-15704821 | None:intergenic | 35.0% | |
| TGTTCACTCATATATTCAGG+GGG | - | chr2_4:15704874-15704893 | None:intergenic | 35.0% | |
| TAAAGGTCATAGTTGTGCAA+GGG | + | chr2_4:15704964-15704983 | Msa0317750:CDS | 35.0% | |
| ATTGCTGATCAGAAACTGTT+TGG | + | chr2_4:15705136-15705155 | Msa0317750:intron | 35.0% | |
| TTGCTGATCAGAAACTGTTT+GGG | + | chr2_4:15705137-15705156 | Msa0317750:intron | 35.0% | |
| AATACACTTAACATGCCAAC+AGG | - | chr2_4:15705184-15705203 | None:intergenic | 35.0% | |
| CAAACAGCTATTAATGACCT+TGG | - | chr2_4:15705355-15705374 | None:intergenic | 35.0% | |
| !!! | ATTGGTCTTTTTTGCTTGGA+AGG | + | chr2_4:15705474-15705493 | Msa0317750:intron | 35.0% |
| !! | TAAGCACTGATGAGATTGTT+TGG | + | chr2_4:15705556-15705575 | Msa0317750:intron | 35.0% |
| !! | GCTGTTTTCATAAGCTATCA+TGG | - | chr2_4:15705646-15705665 | None:intergenic | 35.0% |
| !!! | CTGAGTTGCATTTTGACTAT+AGG | + | chr2_4:15705768-15705787 | Msa0317750:intron | 35.0% |
| !! | GACATTTTAAGAGTCTCTTC+CGG | + | chr2_4:15705883-15705902 | Msa0317750:CDS | 35.0% |
| CTTCCTTAATGTTTGTTCAC+CGG | - | chr2_4:15705905-15705924 | None:intergenic | 35.0% | |
| TTGTGCAGAATGTTTGGTTA+AGG | + | chr2_4:15705989-15706008 | Msa0317750:CDS | 35.0% | |
| !!! | CACTATTTTGAAGTAGCAAC+AGG | - | chr2_4:15706066-15706085 | None:intergenic | 35.0% |
| CTACTTCAAAATAGTGCAGA+GGG | + | chr2_4:15706070-15706089 | Msa0317750:exon | 35.0% | |
| GAATTTATGGAAGGGATTGG+TGG | - | chr2_4:15704058-15704077 | None:intergenic | 40.0% | |
| TTCATCTCTGACATACTTGG+TGG | + | chr2_4:15704237-15704256 | Msa0317750:intron | 40.0% | |
| AAGGGAAATAGTTTCCACCA+TGG | + | chr2_4:15704782-15704801 | Msa0317750:intron | 40.0% | |
| GTTCACTCATATATTCAGGG+GGG | - | chr2_4:15704873-15704892 | None:intergenic | 40.0% | |
| TGAACAATTAGGGCAGCTAA+AGG | + | chr2_4:15704947-15704966 | Msa0317750:CDS | 40.0% | |
| CTAAAGGTCATAGTTGTGCA+AGG | + | chr2_4:15704963-15704982 | Msa0317750:CDS | 40.0% | |
| TCATAGTTGTGCAAGGGAAA+AGG | + | chr2_4:15704970-15704989 | Msa0317750:CDS | 40.0% | |
| GAGAACAACATAAGGATCAC+TGG | - | chr2_4:15705018-15705037 | None:intergenic | 40.0% | |
| ATAGCTGTTTGAATCCGGTT+TGG | + | chr2_4:15705364-15705383 | Msa0317750:CDS | 40.0% | |
| TTCAGCTCTTCATTCCAAAC+CGG | - | chr2_4:15705381-15705400 | None:intergenic | 40.0% | |
| TTTCTCTTTGACAGAACCCT+TGG | + | chr2_4:15705401-15705420 | Msa0317750:CDS | 40.0% | |
| TTCTCTTTGACAGAACCCTT+GGG | + | chr2_4:15705402-15705421 | Msa0317750:CDS | 40.0% | |
| ACCAAATTGAGAACTCCCAA+GGG | - | chr2_4:15705420-15705439 | None:intergenic | 40.0% | |
| CTTCCGGTGAACAAACATTA+AGG | + | chr2_4:15705899-15705918 | Msa0317750:CDS | 40.0% | |
| CGGTGAACAAACATTAAGGA+AGG | + | chr2_4:15705903-15705922 | Msa0317750:CDS | 40.0% | |
| ! | ACAAGACAGTTTTCAGAGTC+AGG | - | chr2_4:15705934-15705953 | None:intergenic | 40.0% |
| TATCAATTGTGTTAACGGCG+CGG | + | chr2_4:15705966-15705985 | Msa0317750:CDS | 40.0% | |
| GAGTGTCAATTCTATCTCTC+CGG | - | chr2_4:15706031-15706050 | None:intergenic | 40.0% | |
| !!! | TTTTGAAGTAGCAACAGGTG+TGG | - | chr2_4:15706061-15706080 | None:intergenic | 40.0% |
| GCTACTTCAAAATAGTGCAG+AGG | + | chr2_4:15706069-15706088 | Msa0317750:exon | 40.0% | |
| GTTGTGATTTATGGCTTCTC+TGG | + | chr2_4:15706259-15706278 | Msa0317750:three_prime_UTR | 40.0% | |
| !!! | GTTTTTTCACCTGCAGCTAC+AGG | - | chr2_4:15704017-15704036 | None:intergenic | 45.0% |
| AGTTGTGCAAGGGAAAAGGT+TGG | + | chr2_4:15704974-15704993 | Msa0317750:CDS | 45.0% | |
| !! | AAGGGAAAAGGTTGGTGATC+CGG | + | chr2_4:15704982-15705001 | Msa0317750:CDS | 45.0% |
| !! | GATCACTGGTCTTGAAATCC+CGG | - | chr2_4:15705004-15705023 | None:intergenic | 45.0% |
| GTAACAGACTGCAAAGACCA+AGG | + | chr2_4:15705335-15705354 | Msa0317750:intron | 45.0% | |
| ACCCTTGGGAGTTCTCAATT+TGG | + | chr2_4:15705416-15705435 | Msa0317750:CDS | 45.0% | |
| CACCAAATTGAGAACTCCCA+AGG | - | chr2_4:15705421-15705440 | None:intergenic | 45.0% | |
| AAGCTATCATGGAGAGCTTG+TGG | - | chr2_4:15705635-15705654 | None:intergenic | 45.0% | |
| CTGCAGAGACTAATGGTTGA+AGG | - | chr2_4:15705852-15705871 | None:intergenic | 45.0% | |
| AATCGAGCTGCAGAGACTAA+TGG | - | chr2_4:15705859-15705878 | None:intergenic | 45.0% | |
| TCTCTCCGGATTCTACTTCA+CGG | - | chr2_4:15706017-15706036 | None:intergenic | 45.0% | |
| ! | TTTTCACCTGCAGCTACAGG+TGG | - | chr2_4:15704014-15704033 | None:intergenic | 50.0% |
| TTGGTGGAACGTGAAGAAGC+AGG | - | chr2_4:15704042-15704061 | None:intergenic | 50.0% | |
| !! | AGGGAAAAGGTTGGTGATCC+GGG | + | chr2_4:15704983-15705002 | Msa0317750:CDS | 50.0% |
| AGGCTCCGTGAAGTAGAATC+CGG | + | chr2_4:15706009-15706028 | Msa0317750:CDS | 50.0% | |
| CTGCAGCTACAGGTGGTCAA+AGG | - | chr2_4:15704007-15704026 | None:intergenic | 55.0% | |
| CTTTGACCACCTGTAGCTGC+AGG | + | chr2_4:15704005-15704024 | Msa0317750:five_prime_UTR | 55.0% | |
| GCGCGGTTGTGCAGAATGTT+TGG | + | chr2_4:15705983-15706002 | Msa0317750:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_4 | gene | 15703993 | 15706467 | 15703993 | ID=Msa0317750;Name=Msa0317750 |
| chr2_4 | mRNA | 15703993 | 15706467 | 15703993 | ID=Msa0317750-mRNA-1;Parent=Msa0317750;Name=Msa0317750-mRNA-1;_AED=0.12;_eAED=0.12;_QI=147|1|1|1|0.66|0.5|4|384|166 |
| chr2_4 | exon | 15703993 | 15704131 | 15703993 | ID=Msa0317750-mRNA-1:exon:5948;Parent=Msa0317750-mRNA-1 |
| chr2_4 | exon | 15704934 | 15705052 | 15704934 | ID=Msa0317750-mRNA-1:exon:5949;Parent=Msa0317750-mRNA-1 |
| chr2_4 | exon | 15705342 | 15705437 | 15705342 | ID=Msa0317750-mRNA-1:exon:5950;Parent=Msa0317750-mRNA-1 |
| chr2_4 | exon | 15705790 | 15706467 | 15705790 | ID=Msa0317750-mRNA-1:exon:5951;Parent=Msa0317750-mRNA-1 |
| chr2_4 | five_prime_UTR | 15703993 | 15704131 | 15703993 | ID=Msa0317750-mRNA-1:five_prime_utr;Parent=Msa0317750-mRNA-1 |
| chr2_4 | five_prime_UTR | 15704934 | 15704941 | 15704934 | ID=Msa0317750-mRNA-1:five_prime_utr;Parent=Msa0317750-mRNA-1 |
| chr2_4 | CDS | 15704942 | 15705052 | 15704942 | ID=Msa0317750-mRNA-1:cds;Parent=Msa0317750-mRNA-1 |
| chr2_4 | CDS | 15705342 | 15705437 | 15705342 | ID=Msa0317750-mRNA-1:cds;Parent=Msa0317750-mRNA-1 |
| chr2_4 | CDS | 15705790 | 15706083 | 15705790 | ID=Msa0317750-mRNA-1:cds;Parent=Msa0317750-mRNA-1 |
| chr2_4 | three_prime_UTR | 15706084 | 15706467 | 15706084 | ID=Msa0317750-mRNA-1:three_prime_utr;Parent=Msa0317750-mRNA-1 |
| Gene Sequence |
| Protein sequence |
