AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0443920


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0443920 MtrunA17_Chr3g0109561 97.059 68 2 0 6 73 15 82 1.42e-42 132
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0443920 AT4G21192.1 71.642 67 19 0 5 71 14 80 3.59e-32 106
Msa0443920 AT4G21192.2 71.642 67 19 0 5 71 14 80 3.59e-32 106

Find 28 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATTTGATTTAACATATT+TGG 0.039226 3_3:+16140522 Msa0443920:three_prime_UTR
AAACATGTTCCTTCATTATC+AGG 0.186242 3_3:-16140662 None:intergenic
AAATTTGATTTAACATATTT+GGG 0.189242 3_3:+16140523 Msa0443920:three_prime_UTR
GTGCCTAAATACTAGAAATT+TGG 0.228840 3_3:-16140463 None:intergenic
CATCCTATTGCAAAATTCTT+TGG 0.235808 3_3:+16139913 Msa0443920:CDS
TACAGAATTGAAAATAAAAT+TGG 0.247760 3_3:+16139942 Msa0443920:CDS
CGACCAAATTTCTAGTATTT+AGG 0.248948 3_3:+16140460 Msa0443920:three_prime_UTR
TCAGATACCTATCATATAAT+AGG 0.331996 3_3:+16140394 Msa0443920:three_prime_UTR
GTGTGTGCAAGCACTATAAT+TGG 0.371757 3_3:+16140563 Msa0443920:three_prime_UTR
ACATCATCCTATTATATGAT+AGG 0.400607 3_3:-16140401 None:intergenic
CAGCAGTCAATAGAAATAGA+AGG 0.433741 3_3:+16140190 Msa0443920:exon
TTTATTATTATTAGATTAGA+GGG 0.456234 3_3:-16140501 None:intergenic
AAAGCCATTATACAAAACTA+AGG 0.469743 3_3:-16140333 None:intergenic
CGAGCCATTGTACAAAACTA+AGG 0.498044 3_3:-16140260 None:intergenic
AACATGTTCCTTCATTATCA+GGG 0.501968 3_3:-16140661 None:intergenic
GCATACTTTAATTCATGTTG+TGG 0.514552 3_3:+16139848 None:intergenic
TGACACGAGTTAGTCTTGTA+TGG 0.542802 3_3:-16140594 None:intergenic
GTGATTATACAAGACTAGTT+CGG 0.549058 3_3:-16140438 None:intergenic
ACAGAAAGCTGTGAAGAGAA+AGG 0.562696 3_3:+16140100 Msa0443920:intron
TGAAAAGAGCAAAGAACTCA+AGG 0.575643 3_3:+16140130 Msa0443920:CDS
GATACCACCCCTGATAATGA+AGG 0.582672 3_3:+16140653 Msa0443920:three_prime_UTR
TGTTCCTTCATTATCAGGGG+TGG 0.590108 3_3:-16140657 None:intergenic
TATTATTATTAGATTAGAGG+GGG 0.596285 3_3:-16140499 None:intergenic
AATTTGATTTAACATATTTG+GGG 0.605428 3_3:+16140524 Msa0443920:three_prime_UTR
TTATTATTATTAGATTAGAG+GGG 0.611399 3_3:-16140500 None:intergenic
ACATGTTCCTTCATTATCAG+GGG 0.637022 3_3:-16140660 None:intergenic
GTGGTTAATGCAGATTATCG+AGG 0.641500 3_3:+16139867 Msa0443920:CDS
GGAGTTTCAGAAGTGTCATG+TGG 0.660393 3_3:+16139888 Msa0443920:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATTATTATTAGATTAGA+GGG - chr3_3:16140504-16140523 None:intergenic 10.0%
!!! TTTTATTATTATTAGATTAG+AGG - chr3_3:16140505-16140524 None:intergenic 10.0%
!!! AAAATTTGATTTAACATATT+TGG + chr3_3:16140522-16140541 Msa0443920:three_prime_UTR 10.0%
!!! AAATTTGATTTAACATATTT+GGG + chr3_3:16140523-16140542 Msa0443920:three_prime_UTR 10.0%
!! TTTATTATTATTAGATTAGA+GGG - chr3_3:16140504-16140523 None:intergenic 10.0%
!!! TTTTATTATTATTAGATTAG+AGG - chr3_3:16140505-16140524 None:intergenic 10.0%
!!! AAAATTTGATTTAACATATT+TGG + chr3_3:16140522-16140541 Msa0443920:three_prime_UTR 10.0%
!!! AAATTTGATTTAACATATTT+GGG + chr3_3:16140523-16140542 Msa0443920:three_prime_UTR 10.0%
!! TACAGAATTGAAAATAAAAT+TGG + chr3_3:16139942-16139961 Msa0443920:CDS 15.0%
!! GAACTTTAACATAAAAAATA+AGG - chr3_3:16140021-16140040 None:intergenic 15.0%
!! TTATTATTATTAGATTAGAG+GGG - chr3_3:16140503-16140522 None:intergenic 15.0%
!!! AATTTGATTTAACATATTTG+GGG + chr3_3:16140524-16140543 Msa0443920:three_prime_UTR 15.0%
!! TACAGAATTGAAAATAAAAT+TGG + chr3_3:16139942-16139961 Msa0443920:CDS 15.0%
!! GAACTTTAACATAAAAAATA+AGG - chr3_3:16140021-16140040 None:intergenic 15.0%
!! TTATTATTATTAGATTAGAG+GGG - chr3_3:16140503-16140522 None:intergenic 15.0%
!!! AATTTGATTTAACATATTTG+GGG + chr3_3:16140524-16140543 Msa0443920:three_prime_UTR 15.0%
!! ATGGAAAAAATTGATACATA+TGG - chr3_3:16140299-16140318 None:intergenic 20.0%
!! TATTATTATTAGATTAGAGG+GGG - chr3_3:16140502-16140521 None:intergenic 20.0%
!! ATGGAAAAAATTGATACATA+TGG - chr3_3:16140299-16140318 None:intergenic 20.0%
!! TATTATTATTAGATTAGAGG+GGG - chr3_3:16140502-16140521 None:intergenic 20.0%
!! TAAGGATTGTATTTTGCAAA+TGG - chr3_3:16140318-16140337 None:intergenic 25.0%
!!! CAATCCTTAGTTTTGTATAA+TGG + chr3_3:16140329-16140348 Msa0443920:three_prime_UTR 25.0%
! AAAGCCATTATACAAAACTA+AGG - chr3_3:16140336-16140355 None:intergenic 25.0%
! TCAGATACCTATCATATAAT+AGG + chr3_3:16140394-16140413 Msa0443920:three_prime_UTR 25.0%
! ACATCATCCTATTATATGAT+AGG - chr3_3:16140404-16140423 None:intergenic 25.0%
!! TAAGGATTGTATTTTGCAAA+TGG - chr3_3:16140318-16140337 None:intergenic 25.0%
!!! CAATCCTTAGTTTTGTATAA+TGG + chr3_3:16140329-16140348 Msa0443920:three_prime_UTR 25.0%
! AAAGCCATTATACAAAACTA+AGG - chr3_3:16140336-16140355 None:intergenic 25.0%
! TCAGATACCTATCATATAAT+AGG + chr3_3:16140394-16140413 Msa0443920:three_prime_UTR 25.0%
! ACATCATCCTATTATATGAT+AGG - chr3_3:16140404-16140423 None:intergenic 25.0%
CATCCTATTGCAAAATTCTT+TGG + chr3_3:16139913-16139932 Msa0443920:CDS 30.0%
AGATGAAAATTACTTACCAC+AGG - chr3_3:16140070-16140089 None:intergenic 30.0%
! TTCTTTGCTCTTTTCAAAGT+TGG - chr3_3:16140126-16140145 None:intergenic 30.0%
!!! CAATCCTTAGTTTTGTACAA+TGG + chr3_3:16140256-16140275 Msa0443920:three_prime_UTR 30.0%
! GTGATTATACAAGACTAGTT+CGG - chr3_3:16140441-16140460 None:intergenic 30.0%
!! CGACCAAATTTCTAGTATTT+AGG + chr3_3:16140460-16140479 Msa0443920:three_prime_UTR 30.0%
GTGCCTAAATACTAGAAATT+TGG - chr3_3:16140466-16140485 None:intergenic 30.0%
AACATGTTCCTTCATTATCA+GGG - chr3_3:16140664-16140683 None:intergenic 30.0%
AAACATGTTCCTTCATTATC+AGG - chr3_3:16140665-16140684 None:intergenic 30.0%
CATCCTATTGCAAAATTCTT+TGG + chr3_3:16139913-16139932 Msa0443920:CDS 30.0%
AGATGAAAATTACTTACCAC+AGG - chr3_3:16140070-16140089 None:intergenic 30.0%
! TTCTTTGCTCTTTTCAAAGT+TGG - chr3_3:16140126-16140145 None:intergenic 30.0%
!!! CAATCCTTAGTTTTGTACAA+TGG + chr3_3:16140256-16140275 Msa0443920:three_prime_UTR 30.0%
! GTGATTATACAAGACTAGTT+CGG - chr3_3:16140441-16140460 None:intergenic 30.0%
!! CGACCAAATTTCTAGTATTT+AGG + chr3_3:16140460-16140479 Msa0443920:three_prime_UTR 30.0%
GTGCCTAAATACTAGAAATT+TGG - chr3_3:16140466-16140485 None:intergenic 30.0%
AACATGTTCCTTCATTATCA+GGG - chr3_3:16140664-16140683 None:intergenic 30.0%
AAACATGTTCCTTCATTATC+AGG - chr3_3:16140665-16140684 None:intergenic 30.0%
! TCGCCAAAGAATTTTGCAAT+AGG - chr3_3:16139919-16139938 None:intergenic 35.0%
!! CTTTGCTTTGAAGTTTGATC+TGG + chr3_3:16140040-16140059 Msa0443920:intron 35.0%
TGAAAAGAGCAAAGAACTCA+AGG + chr3_3:16140130-16140149 Msa0443920:CDS 35.0%
! AGGAACAATTGCGAGTTTTA+AGG + chr3_3:16140150-16140169 Msa0443920:CDS 35.0%
CAGCAGTCAATAGAAATAGA+AGG + chr3_3:16140190-16140209 Msa0443920:exon 35.0%
ACATGTTCCTTCATTATCAG+GGG - chr3_3:16140663-16140682 None:intergenic 35.0%
! TCGCCAAAGAATTTTGCAAT+AGG - chr3_3:16139919-16139938 None:intergenic 35.0%
!! CTTTGCTTTGAAGTTTGATC+TGG + chr3_3:16140040-16140059 Msa0443920:intron 35.0%
TGAAAAGAGCAAAGAACTCA+AGG + chr3_3:16140130-16140149 Msa0443920:CDS 35.0%
! AGGAACAATTGCGAGTTTTA+AGG + chr3_3:16140150-16140169 Msa0443920:CDS 35.0%
CAGCAGTCAATAGAAATAGA+AGG + chr3_3:16140190-16140209 Msa0443920:exon 35.0%
ACATGTTCCTTCATTATCAG+GGG - chr3_3:16140663-16140682 None:intergenic 35.0%
GTGGTTAATGCAGATTATCG+AGG + chr3_3:16139867-16139886 Msa0443920:CDS 40.0%
!!! AGTTTGATCTGGTTTTCCTG+TGG + chr3_3:16140051-16140070 Msa0443920:intron 40.0%
TTCTCTTCACAGCTTTCTGT+TGG - chr3_3:16140100-16140119 None:intergenic 40.0%
ACAGAAAGCTGTGAAGAGAA+AGG + chr3_3:16140100-16140119 Msa0443920:intron 40.0%
CGAGCCATTGTACAAAACTA+AGG - chr3_3:16140263-16140282 None:intergenic 40.0%
GTGTGTGCAAGCACTATAAT+TGG + chr3_3:16140563-16140582 Msa0443920:three_prime_UTR 40.0%
!! TGACACGAGTTAGTCTTGTA+TGG - chr3_3:16140597-16140616 None:intergenic 40.0%
GTGGTTAATGCAGATTATCG+AGG + chr3_3:16139867-16139886 Msa0443920:CDS 40.0%
!!! AGTTTGATCTGGTTTTCCTG+TGG + chr3_3:16140051-16140070 Msa0443920:intron 40.0%
TTCTCTTCACAGCTTTCTGT+TGG - chr3_3:16140100-16140119 None:intergenic 40.0%
ACAGAAAGCTGTGAAGAGAA+AGG + chr3_3:16140100-16140119 Msa0443920:intron 40.0%
CGAGCCATTGTACAAAACTA+AGG - chr3_3:16140263-16140282 None:intergenic 40.0%
GTGTGTGCAAGCACTATAAT+TGG + chr3_3:16140563-16140582 Msa0443920:three_prime_UTR 40.0%
!! TGACACGAGTTAGTCTTGTA+TGG - chr3_3:16140597-16140616 None:intergenic 40.0%
!! GGAGTTTCAGAAGTGTCATG+TGG + chr3_3:16139888-16139907 Msa0443920:CDS 45.0%
! GATACCACCCCTGATAATGA+AGG + chr3_3:16140653-16140672 Msa0443920:three_prime_UTR 45.0%
TGTTCCTTCATTATCAGGGG+TGG - chr3_3:16140660-16140679 None:intergenic 45.0%
!! GGAGTTTCAGAAGTGTCATG+TGG + chr3_3:16139888-16139907 Msa0443920:CDS 45.0%
! GATACCACCCCTGATAATGA+AGG + chr3_3:16140653-16140672 Msa0443920:three_prime_UTR 45.0%
TGTTCCTTCATTATCAGGGG+TGG - chr3_3:16140660-16140679 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 16139862 16140688 16139862 ID=Msa0443920;Name=Msa0443920
chr3_3 mRNA 16139862 16140688 16139862 ID=Msa0443920-mRNA-1;Parent=Msa0443920;Name=Msa0443920-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|1|0.5|1|1|1|2|486|73
chr3_3 exon 16139862 16139984 16139862 ID=Msa0443920-mRNA-1:exon:3409;Parent=Msa0443920-mRNA-1
chr3_3 exon 16140104 16140688 16140104 ID=Msa0443920-mRNA-1:exon:3410;Parent=Msa0443920-mRNA-1
chr3_3 CDS 16139862 16139984 16139862 ID=Msa0443920-mRNA-1:cds;Parent=Msa0443920-mRNA-1
chr3_3 CDS 16140104 16140202 16140104 ID=Msa0443920-mRNA-1:cds;Parent=Msa0443920-mRNA-1
chr3_3 three_prime_UTR 16140203 16140688 16140203 ID=Msa0443920-mRNA-1:three_prime_utr;Parent=Msa0443920-mRNA-1
Gene Sequence

>Msa0443920

ATGTTGTGGTTAATGCAGATTATCGAGGAGTTTCAGAAGTGTCATGTGGAACATCCTATTGCAAAATTCTTTGGCGAATGTACAGAATTGAAAATAAAATTGGATCGCTGCTTCAGAGCAGAAAAAGCTGTGAAGAGAAAGGCCAACTTTGAAAAGAGCAAAGAACTCAAGGAACAATTGCGAGTTTTAAGGAAAGAAAATGCTGCAAGCAGCAGTCAATAG

Protein sequence

>Msa0443920

MLWLMQIIEEFQKCHVEHPIAKFFGECTELKIKLDRCFRAEKAVKRKANFEKSKELKEQLRVLRKENAASSSQ*