AlfalfaGEDB | Msa0630830

M. sativa cultivar zhongmu-4 / Msa0630830

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0630830	AES88520.1	98.551	69	1	0	1	69	1	69	6.95e-42	145

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0008410	Msa0630830	0.844808	6.009369e-59	-8.615850e-47
Msa0017510	Msa0630830	0.806617	7.539603e-50	-8.615850e-47
Msa0064350	Msa0630830	0.852268	5.161384e-61	-8.615850e-47
Msa0072370	Msa0630830	0.811403	7.126216e-51	-8.615850e-47
Msa0113970	Msa0630830	0.805669	1.194156e-49	-8.615850e-47
Msa0120040	Msa0630830	-0.803377	3.589063e-49	-8.615850e-47
Msa0626010	Msa0630830	0.813198	2.890097e-51	-8.615850e-47
Msa0630830	Msa0641190	0.808191	3.496305e-50	-8.615850e-47
Msa0630830	Msa0663900	0.827532	1.485200e-54	-8.615850e-47
Msa0630830	Msa0667050	0.806788	6.938725e-50	-8.615850e-47
Msa0630830	Msa0692840	0.828615	8.148573e-55	-8.615850e-47
Msa0630830	Msa0703230	0.808187	3.502836e-50	-8.615850e-47
Msa0630830	Msa0709500	0.805824	1.107710e-49	-8.615850e-47
Msa0630830	Msa0724830	0.802872	4.565502e-49	-8.615850e-47
Msa0630830	Msa0750880	0.811841	5.720967e-51	-8.615850e-47
Msa0630830	Msa0761940	0.808635	2.811482e-50	-8.615850e-47
Msa0630830	Msa0765380	0.803089	4.117639e-49	-8.615850e-47
Msa0630830	Msa0790850	0.800528	1.381683e-48	-8.615850e-47
Msa0630830	Msa0869100	0.814198	1.740701e-51	-8.615850e-47
Msa0630830	Msa0880140	0.804592	2.006474e-49	-8.615850e-47
Msa0630830	Msa0902760	0.807111	5.928593e-50	-8.615850e-47
Msa0630830	Msa0933230	0.804861	1.762655e-49	-8.615850e-47
Msa0630830	Msa0933310	0.803991	2.676022e-49	-8.615850e-47
Msa0630830	Msa0967370	0.809604	1.743053e-50	-8.615850e-47
Msa0630830	Msa0968590	0.813039	3.131042e-51	-8.615850e-47
Msa0630830	Msa1013040	0.821992	3.005762e-53	-8.615850e-47
Msa0630830	Msa1013400	0.812650	3.810035e-51	-8.615850e-47
Msa0630830	Msa1013770	0.808172	3.528833e-50	-8.615850e-47
Msa0630830	Msa1093530	0.805937	1.048656e-49	-8.615850e-47
Msa0630830	Msa1093840	0.832359	9.887899e-56	-8.615850e-47
Msa0630830	Msa1100720	0.815733	7.947543e-52	-8.615850e-47
Msa0630830	Msa1125760	0.803660	3.134936e-49	-8.615850e-47
Msa0630830	Msa1126770	0.804295	2.313044e-49	-8.615850e-47
Msa0630830	Msa1127890	0.813689	2.253953e-51	-8.615850e-47
Msa0630830	Msa1165870	0.818881	1.555006e-52	-8.615850e-47
Msa0630830	Msa1171360	0.803873	2.831884e-49	-8.615850e-47
Msa0630830	Msa1172670	0.817944	2.535051e-52	-8.615850e-47
Msa0630830	Msa1187840	0.803932	2.753591e-49	-8.615850e-47
Msa0630830	Msa1207270	0.803795	2.939835e-49	-8.615850e-47
Msa0630830	Msa1214890	0.819825	9.477221e-53	-8.615850e-47
Msa0630830	Msa1218600	0.839333	1.687383e-57	-8.615850e-47
Msa0630830	Msa1221060	0.818254	2.157566e-52	-8.615850e-47
Msa0630830	Msa1227400	0.824911	6.245486e-54	-8.615850e-47
Msa0630830	Msa1249240	0.812743	3.635073e-51	-8.615850e-47
Msa0630830	Msa1251160	0.828758	7.524833e-55	-8.615850e-47
Msa0630830	Msa1254510	0.810189	1.304360e-50	-8.615850e-47
Msa0630830	Msa1283170	0.818675	1.731892e-52	-8.615850e-47
Msa0630830	Msa1294480	0.811641	6.325419e-51	-8.615850e-47
Msa0630830	Msa1295490	-0.804110	2.528656e-49	-8.615850e-47
Msa0630830	Msa1303240	0.807488	4.933471e-50	-8.615850e-47
Msa0630830	Msa1303250	0.838083	3.548850e-57	-8.615850e-47
Msa0630830	Msa1305710	0.811780	5.900482e-51	-8.615850e-47
Msa0630830	Msa1307950	0.803065	4.163690e-49	-8.615850e-47
Msa0630830	Msa1331910	0.835451	1.665301e-56	-8.615850e-47
Msa0630830	Msa1342900	0.801559	8.502571e-49	-8.615850e-47
Msa0630830	Msa1353060	0.805463	1.318997e-49	-8.615850e-47
Msa0630830	Msa1361400	0.805730	1.159449e-49	-8.615850e-47
Msa0630830	Msa1361410	0.820561	6.427024e-53	-8.615850e-47
Msa0630830	Msa1365150	0.822261	2.603933e-53	-8.615850e-47
Msa0630830	Msa1367880	0.834937	2.244558e-56	-8.615850e-47
Msa0630830	Msa1371640	0.810446	1.148079e-50	-8.615850e-47
Msa0630830	Msa1374550	0.803819	2.906451e-49	-8.615850e-47
Msa0630830	Msa1385670	0.804924	1.710062e-49	-8.615850e-47
Msa0630830	Msa1457430	0.803088	4.119469e-49	-8.615850e-47
Msa0140300	Msa0630830	0.810529	1.101837e-50	-8.615850e-47
Msa0142950	Msa0630830	0.801489	8.790941e-49	-8.615850e-47
Msa0148100	Msa0630830	0.813780	2.152619e-51	-8.615850e-47
Msa0157100	Msa0630830	0.840021	1.117432e-57	-8.615850e-47
Msa0157290	Msa0630830	0.806683	7.303535e-50	-8.615850e-47
Msa0186890	Msa0630830	0.805549	1.265240e-49	-8.615850e-47
Msa0197930	Msa0630830	0.817725	2.840763e-52	-8.615850e-47
Msa0205850	Msa0630830	0.817176	3.777967e-52	-8.615850e-47
Msa0216240	Msa0630830	0.816878	4.407509e-52	-8.615850e-47
Msa0233350	Msa0630830	0.803856	2.854355e-49	-8.615850e-47
Msa0372660	Msa0630830	0.806169	9.374346e-50	-8.615850e-47
Msa0387730	Msa0630830	0.810075	1.380605e-50	-8.615850e-47
Msa0426330	Msa0630830	0.803142	4.013519e-49	-8.615850e-47
Msa0449830	Msa0630830	0.804303	2.304594e-49	-8.615850e-47
Msa0270320	Msa0630830	0.802793	4.738816e-49	-8.615850e-47
Msa0287650	Msa0630830	0.815822	7.591804e-52	-8.615850e-47
Msa0300120	Msa0630830	0.805991	1.021876e-49	-8.615850e-47
Msa0311700	Msa0630830	0.823363	1.442030e-53	-8.615850e-47
Msa0317280	Msa0630830	0.805847	1.095260e-49	-8.615850e-47
Msa0317340	Msa0630830	0.826996	1.995847e-54	-8.615850e-47
Msa0333920	Msa0630830	0.834688	2.593615e-56	-8.615850e-47
Msa0334040	Msa0630830	0.811554	6.606316e-51	-8.615850e-47
Msa0484780	Msa0630830	0.808773	2.626873e-50	-8.615850e-47
Msa0507680	Msa0630830	0.801098	1.057014e-48	-8.615850e-47
Msa0527550	Msa0630830	0.820124	8.093183e-53	-8.615850e-47
Msa0541860	Msa0630830	0.806222	9.135617e-50	-8.615850e-47
Msa0552810	Msa0630830	0.833904	4.078888e-56	-8.615850e-47
Msa0560470	Msa0630830	0.807023	6.187648e-50	-8.615850e-47
Msa0577220	Msa0630830	0.822807	1.944480e-53	-8.615850e-47
Msa0588140	Msa0630830	0.804671	1.931452e-49	-8.615850e-47
Msa0590800	Msa0630830	0.804393	2.207621e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0630830	MtrunA17_Chr4g0027941	98.551	69	1	0	1	69	1	69	1.20e-45	140

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0630830	AT5G22875.3	81.159	69	13	1	69	1	69	3.50e-37	119
Msa0630830	AT5G22875.1	81.159	69	13	1	69	1	69	3.50e-37	119
Msa0630830	AT5G22875.2	81.159	69	13	1	69	1	69	3.50e-37	119

Find 25 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAAGAAGAAGTGAATGAATT+TGG	0.210447	4_3:+13373285	Msa0630830:exon
CCAAAGAGCAAAATCTTCTA+TGG	0.221326	4_3:+13372871	None:intergenic
CGAGGACCTTATATTAATAC+TGG	0.230807	4_3:+13373229	Msa0630830:CDS
GATCTCACTTTCAAAAGTTA+TGG	0.306933	4_3:+13373507	Msa0630830:three_prime_UTR
GTATATATGTTAAATGAGTT+TGG	0.322119	4_3:+13373485	Msa0630830:three_prime_UTR
GGCTTTGTTGGATCAATTAC+CGG	0.331164	4_3:-13373184	None:intergenic
TTCTTATAAGTTTCATGATC+AGG	0.354887	4_3:-13373268	None:intergenic
TGTGGTGAAAGTGGCTTTGT+TGG	0.403611	4_3:-13373196	None:intergenic
AATACTGGGTCACGTGATAT+TGG	0.406093	4_3:+13373244	Msa0630830:CDS
CGTGACCCAGTATTAATATA+AGG	0.410120	4_3:-13373235	None:intergenic
ACTTTCAAAAGTTATGGAAA+TGG	0.417663	4_3:+13373513	Msa0630830:three_prime_UTR
TGATTAATCTGTAATCTGTA+TGG	0.437504	4_3:+13373396	Msa0630830:three_prime_UTR
TCACCACAAGCTACATTTCG+AGG	0.438997	4_3:+13373211	Msa0630830:CDS
CGAAATTTAGTAGTTGCTGC+TGG	0.448263	4_3:+13373005	Msa0630830:CDS
TTTGTTTCAAAGTTACGTTC+TGG	0.451732	4_3:-13372900	None:intergenic
TTAACTTACGCCATGTAGAA+AGG	0.474118	4_3:-13373062	None:intergenic
TTGCAACTAACTTAGAGTAT+AGG	0.489919	4_3:+13373369	Msa0630830:three_prime_UTR
ACATGAACTTGATCACACTT+AGG	0.491682	4_3:-13373309	None:intergenic
GTTATGGAAATGGCTCTGTA+TGG	0.507143	4_3:+13373523	Msa0630830:three_prime_UTR
CATGAACTTGATCACACTTA+GGG	0.520447	4_3:-13373308	None:intergenic
CAAAGAGCAAAATCTTCTAT+GGG	0.542683	4_3:+13372872	None:intergenic
CAACTAACTTAGAGTATAGG+TGG	0.580151	4_3:+13373372	Msa0630830:three_prime_UTR
GAGGACCTTATATTAATACT+GGG	0.603505	4_3:+13373230	Msa0630830:CDS
AATGTAGCTTGTGGTGAAAG+TGG	0.617393	4_3:-13373205	None:intergenic
GGTCCTCGAAATGTAGCTTG+TGG	0.650550	4_3:-13373214	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTGATAATTTTCATTTT+TGG	+	chr4_3:13373139-13373158	Msa0630830:intron	10.0%
!!!	TTTTGATAATTTTCATTTTT+GGG	+	chr4_3:13373140-13373159	Msa0630830:intron	10.0%
!!	AACACTCAAAAAATCAATTT+AGG	+	chr4_3:13372950-13372969	Msa0630830:five_prime_UTR	20.0%
!!	ACACTCAAAAAATCAATTTA+GGG	+	chr4_3:13372951-13372970	Msa0630830:five_prime_UTR	20.0%
!!	GTATATATGTTAAATGAGTT+TGG	+	chr4_3:13373485-13373504	Msa0630830:three_prime_UTR	20.0%
!	TTCTTATAAGTTTCATGATC+AGG	-	chr4_3:13373271-13373290	None:intergenic	25.0%
!	TAAGAAGAAGTGAATGAATT+TGG	+	chr4_3:13373285-13373304	Msa0630830:exon	25.0%
!!	CAAGTTCATGTTTTCTTTAT+TGG	+	chr4_3:13373321-13373340	Msa0630830:three_prime_UTR	25.0%
!	TGATTAATCTGTAATCTGTA+TGG	+	chr4_3:13373396-13373415	Msa0630830:three_prime_UTR	25.0%
!	ACTTTCAAAAGTTATGGAAA+TGG	+	chr4_3:13373513-13373532	Msa0630830:three_prime_UTR	25.0%
	TTTGTTTCAAAGTTACGTTC+TGG	-	chr4_3:13372903-13372922	None:intergenic	30.0%
	GAGGACCTTATATTAATACT+GGG	+	chr4_3:13373230-13373249	Msa0630830:CDS	30.0%
	TTGCAACTAACTTAGAGTAT+AGG	+	chr4_3:13373369-13373388	Msa0630830:three_prime_UTR	30.0%
	GATCTCACTTTCAAAAGTTA+TGG	+	chr4_3:13373507-13373526	Msa0630830:three_prime_UTR	30.0%
!	AATGGCTTTTCCTTTCTACA+TGG	+	chr4_3:13373052-13373071	Msa0630830:CDS	35.0%
	TTAACTTACGCCATGTAGAA+AGG	-	chr4_3:13373065-13373084	None:intergenic	35.0%
!!!	TTTTAGTTCGTCGAAGAAAC+CGG	+	chr4_3:13373165-13373184	Msa0630830:intron	35.0%
	CGAGGACCTTATATTAATAC+TGG	+	chr4_3:13373229-13373248	Msa0630830:CDS	35.0%
	CGTGACCCAGTATTAATATA+AGG	-	chr4_3:13373238-13373257	None:intergenic	35.0%
	CATGAACTTGATCACACTTA+GGG	-	chr4_3:13373311-13373330	None:intergenic	35.0%
	ACATGAACTTGATCACACTT+AGG	-	chr4_3:13373312-13373331	None:intergenic	35.0%
	CAACTAACTTAGAGTATAGG+TGG	+	chr4_3:13373372-13373391	Msa0630830:three_prime_UTR	35.0%
	CGAAATTTAGTAGTTGCTGC+TGG	+	chr4_3:13373005-13373024	Msa0630830:CDS	40.0%
!	TTACATGCTTTTGCTTCAGC+AGG	+	chr4_3:13373029-13373048	Msa0630830:CDS	40.0%
!!	GGCTTTGTTGGATCAATTAC+CGG	-	chr4_3:13373187-13373206	None:intergenic	40.0%
!	AATGTAGCTTGTGGTGAAAG+TGG	-	chr4_3:13373208-13373227	None:intergenic	40.0%
	AATACTGGGTCACGTGATAT+TGG	+	chr4_3:13373244-13373263	Msa0630830:CDS	40.0%
!	GTTATGGAAATGGCTCTGTA+TGG	+	chr4_3:13373523-13373542	Msa0630830:three_prime_UTR	40.0%
!	TGCTTTTGCTTCAGCAGGAA+TGG	+	chr4_3:13373034-13373053	Msa0630830:CDS	45.0%
!!	TGTGGTGAAAGTGGCTTTGT+TGG	-	chr4_3:13373199-13373218	None:intergenic	45.0%
	TCACCACAAGCTACATTTCG+AGG	+	chr4_3:13373211-13373230	Msa0630830:CDS	45.0%
	GGTCCTCGAAATGTAGCTTG+TGG	-	chr4_3:13373217-13373236	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	13372873	13373611	13372873	ID=Msa0630830;Name=Msa0630830
chr4_3	mRNA	13372873	13373611	13372873	ID=Msa0630830-mRNA-1;Parent=Msa0630830;Name=Msa0630830-mRNA-1;_AED=0.20;_eAED=0.20;_QI=120\|1\|1\|1\|1\|1\|2\|314\|69
chr4_3	exon	13372873	13373075	13372873	ID=Msa0630830-mRNA-1:exon:3768;Parent=Msa0630830-mRNA-1
chr4_3	exon	13373171	13373611	13373171	ID=Msa0630830-mRNA-1:exon:3769;Parent=Msa0630830-mRNA-1
chr4_3	five_prime_UTR	13372873	13372992	13372873	ID=Msa0630830-mRNA-1:five_prime_utr;Parent=Msa0630830-mRNA-1
chr4_3	CDS	13372993	13373075	13372993	ID=Msa0630830-mRNA-1:cds;Parent=Msa0630830-mRNA-1
chr4_3	CDS	13373171	13373297	13373171	ID=Msa0630830-mRNA-1:cds;Parent=Msa0630830-mRNA-1
chr4_3	three_prime_UTR	13373298	13373611	13373298	ID=Msa0630830-mRNA-1:three_prime_utr;Parent=Msa0630830-mRNA-1

Gene Sequence	>Msa0630830 ATGTCACGAAGACGAAATTTAGTAGTTGCTGCTGGATTACATGCTTTTGCTTCAGCAGGAATGGCTTTTCCTTTCTACATGGCTTCGTCGAAGAAACCGGTAATTGATCCAACAAAGCCACTTTCACCACAAGCTACATTTCGAGGACCTTATATTAATACTGGGTCACGTGATATTGGTCCTGATCATGAAACTTATAAGAAGAAGTGA
Protein sequence	>Msa0630830 MSRRRNLVVAAGLHAFASAGMAFPFYMASSKKPVIDPTKPLSPQATFRGPYINTGSRDIGPDHETYKKK*