AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0081210

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0081210 MtrunA17_Chr1g0193561 98.540 137 2 0 1 137 1 137 2.48e-94 268
Msa0081210 MtrunA17_Chr4g0066341 97.810 137 2 1 1 137 1 136 1.35e-91 261
Msa0081210 MtrunA17_Chr1g0193621 91.489 141 7 2 1 137 1 140 1.32e-86 248
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0081210 AT5G03050.2 58.730 126 47 2 10 132 11 134 1.49e-42 137
Msa0081210 AT5G03050.1 58.730 126 47 2 10 132 11 134 1.49e-42 137

Find 43 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGAATACTTGGGCTGTTT+TGG 0.199624 1_2:+59724656 Msa0081210:three_prime_UTR
AATATCCAATTCATTTCTAT+TGG 0.230794 1_2:+59724685 Msa0081210:three_prime_UTR
ATCAGGGAAAACAATGTTTA+AGG 0.261813 1_2:+59724311 Msa0081210:CDS
ATTCAAAATTCATTCAGATT+TGG 0.295741 1_2:+59722839 Msa0081210:five_prime_UTR
TGCATAACTCTTGGAATACT+TGG 0.322190 1_2:+59724646 Msa0081210:three_prime_UTR
CCACGTTTGGTTAATCTTGT+TGG 0.333278 1_2:+59724769 Msa0081210:three_prime_UTR
GTATCTGAGCTCTTGGAATC+AGG 0.345471 1_2:+59724294 Msa0081210:CDS
ATATCCAATTCATTTCTATT+GGG 0.345862 1_2:+59724686 Msa0081210:three_prime_UTR
CCAAGTTGGGTCTGAAGAAA+TGG 0.369614 1_2:+59724121 Msa0081210:CDS
ACAGCCCAATAGAAATGAAT+TGG 0.384691 1_2:-59724690 None:intergenic
AGCATTAGCTTCCTCATTTG+AGG 0.397998 1_2:-59724541 None:intergenic
TAACCAATGTCAGCTCAAAA+AGG 0.399910 1_2:+59723880 Msa0081210:exon
GGTTAATCTTGTTGGAGGTA+TGG 0.415303 1_2:+59724777 Msa0081210:three_prime_UTR
TATTCATGAGTCACCACGTT+TGG 0.429549 1_2:+59724756 Msa0081210:three_prime_UTR
TTGAGATGAATTTACCGGTT+AGG 0.434359 1_2:-59722928 None:intergenic
CTTGTAGGTATCTGAGCTCT+TGG 0.437315 1_2:+59724287 Msa0081210:intron
CCATTTCTTCAGACCCAACT+TGG 0.441813 1_2:-59724121 None:intergenic
CTAGGTATATGCATAACTCT+TGG 0.455507 1_2:+59724637 Msa0081210:three_prime_UTR
CTGATTGTTGGAGTCAGAGA+AGG 0.467815 1_2:-59722901 None:intergenic
AAGCAGGATTCACAAGGAAC+AGG 0.472958 1_2:+59724473 Msa0081210:intron
CAACAATCAGAAAACCTAAC+CGG 0.491910 1_2:+59722914 Msa0081210:five_prime_UTR
GGTTTGAGACTAACCAAGTT+GGG 0.494830 1_2:+59724108 Msa0081210:CDS
TATCTGAGCTCTTGGAATCA+GGG 0.503680 1_2:+59724295 Msa0081210:CDS
GCATGGACAGTGGAGCTTCA+AGG 0.522029 1_2:+59723905 Msa0081210:CDS
CGTTTGGTTAATCTTGTTGG+AGG 0.523553 1_2:+59724772 Msa0081210:three_prime_UTR
TCAAGGAAACGTATGAAACC+TGG 0.524964 1_2:+59723922 Msa0081210:CDS
ATCGCAGATTGTCCTTGTAC+AGG 0.525946 1_2:+59724079 Msa0081210:CDS
CAAACCCTTCCTCCTGTACA+AGG 0.529759 1_2:-59724091 None:intergenic
ATTGTCCTTGTACAGGAGGA+AGG 0.538219 1_2:+59724086 Msa0081210:CDS
TTGTCCTTGTACAGGAGGAA+GGG 0.558464 1_2:+59724087 Msa0081210:CDS
TTAAAGAATTCAATAAACAT+TGG 0.560206 1_2:-59724863 None:intergenic
CAAGGAAACGTATGAAACCT+GGG 0.567520 1_2:+59723923 Msa0081210:CDS
AGTTGCCTCATGTCATGTCT+AGG 0.572968 1_2:+59724619 Msa0081210:three_prime_UTR
TGATTGTTGGAGTCAGAGAA+GGG 0.577575 1_2:-59722900 None:intergenic
GCATAACTCTTGGAATACTT+GGG 0.586646 1_2:+59724647 Msa0081210:three_prime_UTR
TCAAATAAGCAGGATTCACA+AGG 0.592512 1_2:+59724467 Msa0081210:intron
GGGCTGGCATTTCAGAAACA+AGG 0.596580 1_2:+59724840 Msa0081210:three_prime_UTR
GGGTTTGAGACTAACCAAGT+TGG 0.597415 1_2:+59724107 Msa0081210:CDS
TGCACTTGATGCCTCAAATG+AGG 0.607261 1_2:+59724530 Msa0081210:CDS
GCAGATTGTCCTTGTACAGG+AGG 0.643938 1_2:+59724082 Msa0081210:CDS
CCAACAAGATTAACCAAACG+TGG 0.656398 1_2:-59724769 None:intergenic
AAGGAAACGTATGAAACCTG+GGG 0.689052 1_2:+59723924 Msa0081210:CDS
ATATACCTAGACATGACATG+AGG 0.734630 1_2:-59724624 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCAATATCTGATTAATTTA+TGG + chr1_2:59722996-59723015 Msa0081210:intron 15.0%
!! ATTCATCAATTAAACAAAAA+CGG - chr1_2:59723302-59723321 None:intergenic 15.0%
!!! TTTTAGATTTTGAGTATTTT+TGG + chr1_2:59723356-59723375 Msa0081210:intron 15.0%
!! AAGTTTGAATTATAATTTCT+AGG - chr1_2:59723842-59723861 None:intergenic 15.0%
!! TTAAAGAATTCAATAAACAT+TGG - chr1_2:59724866-59724885 None:intergenic 15.0%
!! TTCAATATCTGATTAATTTA+TGG + chr1_2:59722996-59723015 Msa0081210:intron 15.0%
!! ATTCATCAATTAAACAAAAA+CGG - chr1_2:59723302-59723321 None:intergenic 15.0%
!!! TTTTAGATTTTGAGTATTTT+TGG + chr1_2:59723356-59723375 Msa0081210:intron 15.0%
!! AAGTTTGAATTATAATTTCT+AGG - chr1_2:59723842-59723861 None:intergenic 15.0%
!! TTAAAGAATTCAATAAACAT+TGG - chr1_2:59724866-59724885 None:intergenic 15.0%
!! ATTCAAAATTCATTCAGATT+TGG + chr1_2:59722839-59722858 Msa0081210:five_prime_UTR 20.0%
!! TTCATCAATTAAACAAAAAC+GGG - chr1_2:59723301-59723320 None:intergenic 20.0%
!! CAAATAAGTCAATATAAACA+GGG - chr1_2:59723477-59723496 None:intergenic 20.0%
!! TCAAATAAGTCAATATAAAC+AGG - chr1_2:59723478-59723497 None:intergenic 20.0%
!!! ATGTTTGAAATGTTTAATTG+TGG + chr1_2:59724260-59724279 Msa0081210:intron 20.0%
!! TTTAAACATTGAATGTTCAA+TGG - chr1_2:59724387-59724406 None:intergenic 20.0%
!!! TGTTAGTTTTTGACTTTTTA+TGG + chr1_2:59724427-59724446 Msa0081210:intron 20.0%
!! AATATCCAATTCATTTCTAT+TGG + chr1_2:59724685-59724704 Msa0081210:three_prime_UTR 20.0%
!! ATATCCAATTCATTTCTATT+GGG + chr1_2:59724686-59724705 Msa0081210:three_prime_UTR 20.0%
!! ATTCAAAATTCATTCAGATT+TGG + chr1_2:59722839-59722858 Msa0081210:five_prime_UTR 20.0%
!! TTCATCAATTAAACAAAAAC+GGG - chr1_2:59723301-59723320 None:intergenic 20.0%
!! CAAATAAGTCAATATAAACA+GGG - chr1_2:59723477-59723496 None:intergenic 20.0%
!! TCAAATAAGTCAATATAAAC+AGG - chr1_2:59723478-59723497 None:intergenic 20.0%
!!! ATGTTTGAAATGTTTAATTG+TGG + chr1_2:59724260-59724279 Msa0081210:intron 20.0%
!! TTTAAACATTGAATGTTCAA+TGG - chr1_2:59724387-59724406 None:intergenic 20.0%
!!! TGTTAGTTTTTGACTTTTTA+TGG + chr1_2:59724427-59724446 Msa0081210:intron 20.0%
!! AATATCCAATTCATTTCTAT+TGG + chr1_2:59724685-59724704 Msa0081210:three_prime_UTR 20.0%
!! ATATCCAATTCATTTCTATT+GGG + chr1_2:59724686-59724705 Msa0081210:three_prime_UTR 20.0%
! TATGATATAAGCATTCTGTT+TGG + chr1_2:59723093-59723112 Msa0081210:intron 25.0%
!! GATGAATTTTCTACATGTTT+TGG + chr1_2:59723315-59723334 Msa0081210:intron 25.0%
! AATTCACTTCAAACTTATAC+TGG - chr1_2:59723440-59723459 None:intergenic 25.0%
! AAGTTATTATGAAAGTAAGC+TGG - chr1_2:59723582-59723601 None:intergenic 25.0%
! CAAATAAGCAATTCCAAATA+GGG - chr1_2:59723644-59723663 None:intergenic 25.0%
! TCAAATAAGCAATTCCAAAT+AGG - chr1_2:59723645-59723664 None:intergenic 25.0%
!! CTTTTCTTTTCACAATAAAC+AGG + chr1_2:59724025-59724044 Msa0081210:intron 25.0%
! TTTAATTGTGGATTTCTTGT+AGG + chr1_2:59724272-59724291 Msa0081210:intron 25.0%
!!! CTTTTAACTTTCAAATAAGC+AGG + chr1_2:59724457-59724476 Msa0081210:intron 25.0%
! TATGATATAAGCATTCTGTT+TGG + chr1_2:59723093-59723112 Msa0081210:intron 25.0%
!! GATGAATTTTCTACATGTTT+TGG + chr1_2:59723315-59723334 Msa0081210:intron 25.0%
! AATTCACTTCAAACTTATAC+TGG - chr1_2:59723440-59723459 None:intergenic 25.0%
! AAGTTATTATGAAAGTAAGC+TGG - chr1_2:59723582-59723601 None:intergenic 25.0%
! CAAATAAGCAATTCCAAATA+GGG - chr1_2:59723644-59723663 None:intergenic 25.0%
! TCAAATAAGCAATTCCAAAT+AGG - chr1_2:59723645-59723664 None:intergenic 25.0%
!! CTTTTCTTTTCACAATAAAC+AGG + chr1_2:59724025-59724044 Msa0081210:intron 25.0%
! TTTAATTGTGGATTTCTTGT+AGG + chr1_2:59724272-59724291 Msa0081210:intron 25.0%
!!! CTTTTAACTTTCAAATAAGC+AGG + chr1_2:59724457-59724476 Msa0081210:intron 25.0%
!!! GCGCATTTCAAATTTTGATT+GGG + chr1_2:59722801-59722820 Msa0081210:five_prime_UTR 30.0%
!!! TTTCGATTTGGTTTTTGCAT+TGG + chr1_2:59723116-59723135 Msa0081210:intron 30.0%
! GATTCGAATCTTCAGATTTT+TGG + chr1_2:59723265-59723284 Msa0081210:intron 30.0%
! CTAACATAACTTTTGTGAGA+CGG + chr1_2:59723507-59723526 Msa0081210:intron 30.0%
CTATAAGCTGCAAATTTAAG+TGG + chr1_2:59723802-59723821 Msa0081210:intron 30.0%
!!! CAAACTTGTTTTATACCTGT+AGG + chr1_2:59723855-59723874 Msa0081210:intron 30.0%
ATGACGAGAAAAATGTTGAA+AGG - chr1_2:59723965-59723984 None:intergenic 30.0%
! AGATTGAGAATTTTACTCAG+AGG + chr1_2:59724171-59724190 Msa0081210:CDS 30.0%
! GATTGAGAATTTTACTCAGA+GGG + chr1_2:59724172-59724191 Msa0081210:CDS 30.0%
ATCAGGGAAAACAATGTTTA+AGG + chr1_2:59724311-59724330 Msa0081210:CDS 30.0%
!!! ATCTGTAGCATTTTAGTTTG+AGG + chr1_2:59724819-59724838 Msa0081210:three_prime_UTR 30.0%
!!! TCTGTAGCATTTTAGTTTGA+GGG + chr1_2:59724820-59724839 Msa0081210:three_prime_UTR 30.0%
!!! GCGCATTTCAAATTTTGATT+GGG + chr1_2:59722801-59722820 Msa0081210:five_prime_UTR 30.0%
!!! TTTCGATTTGGTTTTTGCAT+TGG + chr1_2:59723116-59723135 Msa0081210:intron 30.0%
! GATTCGAATCTTCAGATTTT+TGG + chr1_2:59723265-59723284 Msa0081210:intron 30.0%
! CTAACATAACTTTTGTGAGA+CGG + chr1_2:59723507-59723526 Msa0081210:intron 30.0%
CTATAAGCTGCAAATTTAAG+TGG + chr1_2:59723802-59723821 Msa0081210:intron 30.0%
!!! CAAACTTGTTTTATACCTGT+AGG + chr1_2:59723855-59723874 Msa0081210:intron 30.0%
ATGACGAGAAAAATGTTGAA+AGG - chr1_2:59723965-59723984 None:intergenic 30.0%
! AGATTGAGAATTTTACTCAG+AGG + chr1_2:59724171-59724190 Msa0081210:CDS 30.0%
! GATTGAGAATTTTACTCAGA+GGG + chr1_2:59724172-59724191 Msa0081210:CDS 30.0%
ATCAGGGAAAACAATGTTTA+AGG + chr1_2:59724311-59724330 Msa0081210:CDS 30.0%
!!! ATCTGTAGCATTTTAGTTTG+AGG + chr1_2:59724819-59724838 Msa0081210:three_prime_UTR 30.0%
!!! TCTGTAGCATTTTAGTTTGA+GGG + chr1_2:59724820-59724839 Msa0081210:three_prime_UTR 30.0%
!! CGCGCATTTCAAATTTTGAT+TGG + chr1_2:59722800-59722819 Msa0081210:five_prime_UTR 35.0%
CAACAATCAGAAAACCTAAC+CGG + chr1_2:59722914-59722933 Msa0081210:five_prime_UTR 35.0%
! TTGAGATGAATTTACCGGTT+AGG - chr1_2:59722931-59722950 None:intergenic 35.0%
AACGGTTGAGATGAATTTAC+CGG - chr1_2:59722936-59722955 None:intergenic 35.0%
AGAGCGAAGAAGATGAAAAA+CGG - chr1_2:59722954-59722973 None:intergenic 35.0%
! CATTCTGTTTGGTTTCGATT+TGG + chr1_2:59723104-59723123 Msa0081210:intron 35.0%
! ATAACTTTTGTGAGACGGTT+TGG + chr1_2:59723512-59723531 Msa0081210:intron 35.0%
! TAACTTTTGTGAGACGGTTT+GGG + chr1_2:59723513-59723532 Msa0081210:intron 35.0%
AAAACAGCTTAGTCCCTATT+TGG + chr1_2:59723628-59723647 Msa0081210:intron 35.0%
! GTTTTGTGAGACTATTTGAG+AGG + chr1_2:59723683-59723702 Msa0081210:intron 35.0%
TAACCAATGTCAGCTCAAAA+AGG + chr1_2:59723880-59723899 Msa0081210:exon 35.0%
TCAAATAAGCAGGATTCACA+AGG + chr1_2:59724467-59724486 Msa0081210:intron 35.0%
! ACAAGGAACAGGTTGAAAAA+TGG + chr1_2:59724484-59724503 Msa0081210:CDS 35.0%
ATATACCTAGACATGACATG+AGG - chr1_2:59724627-59724646 None:intergenic 35.0%
CTAGGTATATGCATAACTCT+TGG + chr1_2:59724637-59724656 Msa0081210:three_prime_UTR 35.0%
TGCATAACTCTTGGAATACT+TGG + chr1_2:59724646-59724665 Msa0081210:three_prime_UTR 35.0%
GCATAACTCTTGGAATACTT+GGG + chr1_2:59724647-59724666 Msa0081210:three_prime_UTR 35.0%
ACAGCCCAATAGAAATGAAT+TGG - chr1_2:59724693-59724712 None:intergenic 35.0%
!! CGCGCATTTCAAATTTTGAT+TGG + chr1_2:59722800-59722819 Msa0081210:five_prime_UTR 35.0%
CAACAATCAGAAAACCTAAC+CGG + chr1_2:59722914-59722933 Msa0081210:five_prime_UTR 35.0%
! TTGAGATGAATTTACCGGTT+AGG - chr1_2:59722931-59722950 None:intergenic 35.0%
AACGGTTGAGATGAATTTAC+CGG - chr1_2:59722936-59722955 None:intergenic 35.0%
AGAGCGAAGAAGATGAAAAA+CGG - chr1_2:59722954-59722973 None:intergenic 35.0%
! CATTCTGTTTGGTTTCGATT+TGG + chr1_2:59723104-59723123 Msa0081210:intron 35.0%
! ATAACTTTTGTGAGACGGTT+TGG + chr1_2:59723512-59723531 Msa0081210:intron 35.0%
! TAACTTTTGTGAGACGGTTT+GGG + chr1_2:59723513-59723532 Msa0081210:intron 35.0%
AAAACAGCTTAGTCCCTATT+TGG + chr1_2:59723628-59723647 Msa0081210:intron 35.0%
! GTTTTGTGAGACTATTTGAG+AGG + chr1_2:59723683-59723702 Msa0081210:intron 35.0%
TAACCAATGTCAGCTCAAAA+AGG + chr1_2:59723880-59723899 Msa0081210:exon 35.0%
TCAAATAAGCAGGATTCACA+AGG + chr1_2:59724467-59724486 Msa0081210:intron 35.0%
! ACAAGGAACAGGTTGAAAAA+TGG + chr1_2:59724484-59724503 Msa0081210:CDS 35.0%
ATATACCTAGACATGACATG+AGG - chr1_2:59724627-59724646 None:intergenic 35.0%
CTAGGTATATGCATAACTCT+TGG + chr1_2:59724637-59724656 Msa0081210:three_prime_UTR 35.0%
TGCATAACTCTTGGAATACT+TGG + chr1_2:59724646-59724665 Msa0081210:three_prime_UTR 35.0%
GCATAACTCTTGGAATACTT+GGG + chr1_2:59724647-59724666 Msa0081210:three_prime_UTR 35.0%
ACAGCCCAATAGAAATGAAT+TGG - chr1_2:59724693-59724712 None:intergenic 35.0%
TGATTGTTGGAGTCAGAGAA+GGG - chr1_2:59722903-59722922 None:intergenic 40.0%
! CGGTTAGGTTTTCTGATTGT+TGG - chr1_2:59722916-59722935 None:intergenic 40.0%
! TTGCTTGCTAATGCTTTGCT+AGG + chr1_2:59723242-59723261 Msa0081210:intron 40.0%
! TGCTTGCTAATGCTTTGCTA+GGG + chr1_2:59723243-59723262 Msa0081210:intron 40.0%
!! CTTCCTTTTTGAGCTGACAT+TGG - chr1_2:59723886-59723905 None:intergenic 40.0%
TCAAGGAAACGTATGAAACC+TGG + chr1_2:59723922-59723941 Msa0081210:CDS 40.0%
CAAGGAAACGTATGAAACCT+GGG + chr1_2:59723923-59723942 Msa0081210:CDS 40.0%
AAGGAAACGTATGAAACCTG+GGG + chr1_2:59723924-59723943 Msa0081210:CDS 40.0%
GCGAAAATAACAATTACCCC+AGG - chr1_2:59723943-59723962 None:intergenic 40.0%
! GGTTTGAGACTAACCAAGTT+GGG + chr1_2:59724108-59724127 Msa0081210:CDS 40.0%
TATCTGAGCTCTTGGAATCA+GGG + chr1_2:59724295-59724314 Msa0081210:CDS 40.0%
! AGCATTAGCTTCCTCATTTG+AGG - chr1_2:59724544-59724563 None:intergenic 40.0%
! TTGGAATACTTGGGCTGTTT+TGG + chr1_2:59724656-59724675 Msa0081210:three_prime_UTR 40.0%
TATTCATGAGTCACCACGTT+TGG + chr1_2:59724756-59724775 Msa0081210:three_prime_UTR 40.0%
CCAACAAGATTAACCAAACG+TGG - chr1_2:59724772-59724791 None:intergenic 40.0%
CCACGTTTGGTTAATCTTGT+TGG + chr1_2:59724769-59724788 Msa0081210:three_prime_UTR 40.0%
CGTTTGGTTAATCTTGTTGG+AGG + chr1_2:59724772-59724791 Msa0081210:three_prime_UTR 40.0%
GGTTAATCTTGTTGGAGGTA+TGG + chr1_2:59724777-59724796 Msa0081210:three_prime_UTR 40.0%
!!! TAGCATTTTAGTTTGAGGGC+TGG + chr1_2:59724824-59724843 Msa0081210:three_prime_UTR 40.0%
TGATTGTTGGAGTCAGAGAA+GGG - chr1_2:59722903-59722922 None:intergenic 40.0%
! CGGTTAGGTTTTCTGATTGT+TGG - chr1_2:59722916-59722935 None:intergenic 40.0%
! TTGCTTGCTAATGCTTTGCT+AGG + chr1_2:59723242-59723261 Msa0081210:intron 40.0%
! TGCTTGCTAATGCTTTGCTA+GGG + chr1_2:59723243-59723262 Msa0081210:intron 40.0%
!! CTTCCTTTTTGAGCTGACAT+TGG - chr1_2:59723886-59723905 None:intergenic 40.0%
TCAAGGAAACGTATGAAACC+TGG + chr1_2:59723922-59723941 Msa0081210:CDS 40.0%
CAAGGAAACGTATGAAACCT+GGG + chr1_2:59723923-59723942 Msa0081210:CDS 40.0%
AAGGAAACGTATGAAACCTG+GGG + chr1_2:59723924-59723943 Msa0081210:CDS 40.0%
GCGAAAATAACAATTACCCC+AGG - chr1_2:59723943-59723962 None:intergenic 40.0%
! GGTTTGAGACTAACCAAGTT+GGG + chr1_2:59724108-59724127 Msa0081210:CDS 40.0%
TATCTGAGCTCTTGGAATCA+GGG + chr1_2:59724295-59724314 Msa0081210:CDS 40.0%
! AGCATTAGCTTCCTCATTTG+AGG - chr1_2:59724544-59724563 None:intergenic 40.0%
! TTGGAATACTTGGGCTGTTT+TGG + chr1_2:59724656-59724675 Msa0081210:three_prime_UTR 40.0%
TATTCATGAGTCACCACGTT+TGG + chr1_2:59724756-59724775 Msa0081210:three_prime_UTR 40.0%
CCAACAAGATTAACCAAACG+TGG - chr1_2:59724772-59724791 None:intergenic 40.0%
CCACGTTTGGTTAATCTTGT+TGG + chr1_2:59724769-59724788 Msa0081210:three_prime_UTR 40.0%
CGTTTGGTTAATCTTGTTGG+AGG + chr1_2:59724772-59724791 Msa0081210:three_prime_UTR 40.0%
GGTTAATCTTGTTGGAGGTA+TGG + chr1_2:59724777-59724796 Msa0081210:three_prime_UTR 40.0%
!!! TAGCATTTTAGTTTGAGGGC+TGG + chr1_2:59724824-59724843 Msa0081210:three_prime_UTR 40.0%
CTGATTGTTGGAGTCAGAGA+AGG - chr1_2:59722904-59722923 None:intergenic 45.0%
! CTGACATTGGTTAAGCCTAC+AGG - chr1_2:59723873-59723892 None:intergenic 45.0%
GTCAGCTCAAAAAGGAAGCA+TGG + chr1_2:59723888-59723907 Msa0081210:CDS 45.0%
! CAAAAAGGAAGCATGGACAG+TGG + chr1_2:59723895-59723914 Msa0081210:CDS 45.0%
ATCGCAGATTGTCCTTGTAC+AGG + chr1_2:59724079-59724098 Msa0081210:CDS 45.0%
ATTGTCCTTGTACAGGAGGA+AGG + chr1_2:59724086-59724105 Msa0081210:CDS 45.0%
TTGTCCTTGTACAGGAGGAA+GGG + chr1_2:59724087-59724106 Msa0081210:CDS 45.0%
! GGGTTTGAGACTAACCAAGT+TGG + chr1_2:59724107-59724126 Msa0081210:CDS 45.0%
CCATTTCTTCAGACCCAACT+TGG - chr1_2:59724124-59724143 None:intergenic 45.0%
! CCAAGTTGGGTCTGAAGAAA+TGG + chr1_2:59724121-59724140 Msa0081210:CDS 45.0%
CTTGTAGGTATCTGAGCTCT+TGG + chr1_2:59724287-59724306 Msa0081210:intron 45.0%
GTATCTGAGCTCTTGGAATC+AGG + chr1_2:59724294-59724313 Msa0081210:CDS 45.0%
AAGCAGGATTCACAAGGAAC+AGG + chr1_2:59724473-59724492 Msa0081210:intron 45.0%
! GGAACAGGTTGAAAAATGGC+AGG + chr1_2:59724488-59724507 Msa0081210:CDS 45.0%
TGCACTTGATGCCTCAAATG+AGG + chr1_2:59724530-59724549 Msa0081210:CDS 45.0%
AGTTGCCTCATGTCATGTCT+AGG + chr1_2:59724619-59724638 Msa0081210:three_prime_UTR 45.0%
CTGATTGTTGGAGTCAGAGA+AGG - chr1_2:59722904-59722923 None:intergenic 45.0%
! CTGACATTGGTTAAGCCTAC+AGG - chr1_2:59723873-59723892 None:intergenic 45.0%
GTCAGCTCAAAAAGGAAGCA+TGG + chr1_2:59723888-59723907 Msa0081210:CDS 45.0%
! CAAAAAGGAAGCATGGACAG+TGG + chr1_2:59723895-59723914 Msa0081210:CDS 45.0%
ATCGCAGATTGTCCTTGTAC+AGG + chr1_2:59724079-59724098 Msa0081210:CDS 45.0%
ATTGTCCTTGTACAGGAGGA+AGG + chr1_2:59724086-59724105 Msa0081210:CDS 45.0%
TTGTCCTTGTACAGGAGGAA+GGG + chr1_2:59724087-59724106 Msa0081210:CDS 45.0%
! GGGTTTGAGACTAACCAAGT+TGG + chr1_2:59724107-59724126 Msa0081210:CDS 45.0%
CCATTTCTTCAGACCCAACT+TGG - chr1_2:59724124-59724143 None:intergenic 45.0%
! CCAAGTTGGGTCTGAAGAAA+TGG + chr1_2:59724121-59724140 Msa0081210:CDS 45.0%
CTTGTAGGTATCTGAGCTCT+TGG + chr1_2:59724287-59724306 Msa0081210:intron 45.0%
GTATCTGAGCTCTTGGAATC+AGG + chr1_2:59724294-59724313 Msa0081210:CDS 45.0%
AAGCAGGATTCACAAGGAAC+AGG + chr1_2:59724473-59724492 Msa0081210:intron 45.0%
! GGAACAGGTTGAAAAATGGC+AGG + chr1_2:59724488-59724507 Msa0081210:CDS 45.0%
TGCACTTGATGCCTCAAATG+AGG + chr1_2:59724530-59724549 Msa0081210:CDS 45.0%
AGTTGCCTCATGTCATGTCT+AGG + chr1_2:59724619-59724638 Msa0081210:three_prime_UTR 45.0%
GCAGATTGTCCTTGTACAGG+AGG + chr1_2:59724082-59724101 Msa0081210:CDS 50.0%
CAAACCCTTCCTCCTGTACA+AGG - chr1_2:59724094-59724113 None:intergenic 50.0%
! GGGCTGGCATTTCAGAAACA+AGG + chr1_2:59724840-59724859 Msa0081210:three_prime_UTR 50.0%
GCAGATTGTCCTTGTACAGG+AGG + chr1_2:59724082-59724101 Msa0081210:CDS 50.0%
CAAACCCTTCCTCCTGTACA+AGG - chr1_2:59724094-59724113 None:intergenic 50.0%
! GGGCTGGCATTTCAGAAACA+AGG + chr1_2:59724840-59724859 Msa0081210:three_prime_UTR 50.0%
GCATGGACAGTGGAGCTTCA+AGG + chr1_2:59723905-59723924 Msa0081210:CDS 55.0%
GCATGGACAGTGGAGCTTCA+AGG + chr1_2:59723905-59723924 Msa0081210:CDS 55.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 59722764 59724888 59722764 ID=Msa0081210;Name=Msa0081210
chr1_2 mRNA 59722764 59724888 59722764 ID=Msa0081210-mRNA-1;Parent=Msa0081210;Name=Msa0081210-mRNA-1;_AED=0.06;_eAED=0.06;_QI=181|1|1|1|0.75|0.6|5|277|137
chr1_2 exon 59722764 59722935 59722764 ID=Msa0081210-mRNA-1:exon:19371;Parent=Msa0081210-mRNA-1
chr1_2 exon 59723877 59723945 59723877 ID=Msa0081210-mRNA-1:exon:19372;Parent=Msa0081210-mRNA-1
chr1_2 exon 59724047 59724193 59724047 ID=Msa0081210-mRNA-1:exon:19373;Parent=Msa0081210-mRNA-1
chr1_2 exon 59724294 59724367 59724294 ID=Msa0081210-mRNA-1:exon:19374;Parent=Msa0081210-mRNA-1
chr1_2 exon 59724479 59724888 59724479 ID=Msa0081210-mRNA-1:exon:19375;Parent=Msa0081210-mRNA-1
chr1_2 five_prime_UTR 59722764 59722935 59722764 ID=Msa0081210-mRNA-1:five_prime_utr;Parent=Msa0081210-mRNA-1
chr1_2 five_prime_UTR 59723877 59723885 59723877 ID=Msa0081210-mRNA-1:five_prime_utr;Parent=Msa0081210-mRNA-1
chr1_2 CDS 59723886 59723945 59723886 ID=Msa0081210-mRNA-1:cds;Parent=Msa0081210-mRNA-1
chr1_2 CDS 59724047 59724193 59724047 ID=Msa0081210-mRNA-1:cds;Parent=Msa0081210-mRNA-1
chr1_2 CDS 59724294 59724367 59724294 ID=Msa0081210-mRNA-1:cds;Parent=Msa0081210-mRNA-1
chr1_2 CDS 59724479 59724611 59724479 ID=Msa0081210-mRNA-1:cds;Parent=Msa0081210-mRNA-1
chr1_2 three_prime_UTR 59724612 59724888 59724612 ID=Msa0081210-mRNA-1:three_prime_utr;Parent=Msa0081210-mRNA-1
Gene Sequence

>Msa0081210

ATGTCAGCTCAAAAAGGAAGCATGGACAGTGGAGCTTCAAGGAAACGTATGAAACCTGGGGTAGTACAAGAAAAGATTAGAGATGAAGCAGAATCGCAGATTGTCCTTGTACAGGAGGAAGGGTTTGAGACTAACCAAGTTGGGTCTGAAGAAATGGAGCTCAATATTTCTCTTGTTCTTGAGAAGATTGAGAATTTTACTCAGAGGGTATCTGAGCTCTTGGAATCAGGGAAAACAATGTTTAAGGAGCTATGCAATGAATTTGAAGAGAAACTGATTATGATTCACAAGGAACAGGTTGAAAAATGGCAGGAAGAGATCAAAGAACTGCGTGCACTTGATGCCTCAAATGAGGAAGCTAATGCTCTTCTGCAAAATGCACGATATGTACTTCAGCTAACTCGTAATGATTAG
Protein sequence

>Msa0081210

MSAQKGSMDSGASRKRMKPGVVQEKIRDEAESQIVLVQEEGFETNQVGSEEMELNISLVLEKIENFTQRVSELLESGKTMFKELCNEFEEKLIMIHKEQVEKWQEEIKELRALDASNEEANALLQNARYVLQLTRND*