Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1402000 | XP_003591211.1 | 100.000 | 165 | 0 | 0 | 1 | 165 | 1 | 165 | 2.33e-119 | 343 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1402000 | sp|Q8LGG8|USPAL_ARATH | 31.944 | 144 | 93 | 4 | 24 | 164 | 30 | 171 | 1.54e-16 | 75.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1402000 | G7I9H1 | 100.000 | 165 | 0 | 0 | 1 | 165 | 1 | 165 | 1.11e-119 | 343 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0032040 | Msa1402000 | -0.802848 | 4.616459e-49 | -8.615850e-47 |
| Msa0039270 | Msa1402000 | 0.818102 | 2.335724e-52 | -8.615850e-47 |
| Msa0043840 | Msa1402000 | 0.809597 | 1.748748e-50 | -8.615850e-47 |
| Msa0064960 | Msa1402000 | 0.809160 | 2.170286e-50 | -8.615850e-47 |
| Msa0080290 | Msa1402000 | 0.863814 | 1.902646e-64 | -8.615850e-47 |
| Msa0080360 | Msa1402000 | 0.867429 | 1.377489e-65 | -8.615850e-47 |
| Msa0090500 | Msa1402000 | 0.810501 | 1.117160e-50 | -8.615850e-47 |
| Msa1373920 | Msa1402000 | -0.801231 | 9.925839e-49 | -8.615850e-47 |
| Msa1379250 | Msa1402000 | 0.828169 | 1.044129e-54 | -8.615850e-47 |
| Msa1384980 | Msa1402000 | -0.817901 | 2.592259e-52 | -8.615850e-47 |
| Msa1400940 | Msa1402000 | 0.838873 | 2.220544e-57 | -8.615850e-47 |
| Msa1402000 | Msa1407210 | 0.809982 | 1.445526e-50 | -8.615850e-47 |
| Msa1402000 | Msa1409060 | 0.836529 | 8.872705e-57 | -8.615850e-47 |
| Msa1402000 | Msa1458370 | 0.803614 | 3.204759e-49 | -8.615850e-47 |
| Msa1402000 | Msa1463500 | -0.800407 | 1.462328e-48 | -8.615850e-47 |
| Msa0648410 | Msa1402000 | -0.807335 | 5.314715e-50 | -8.615850e-47 |
| Msa0690920 | Msa1402000 | -0.804096 | 2.544764e-49 | -8.615850e-47 |
| Msa0709820 | Msa1402000 | 0.852957 | 3.281867e-61 | -8.615850e-47 |
| Msa0712440 | Msa1402000 | -0.811863 | 5.657931e-51 | -8.615850e-47 |
| Msa0715260 | Msa1402000 | 0.800238 | 1.583115e-48 | -8.615850e-47 |
| Msa0716600 | Msa1402000 | 0.810189 | 1.304742e-50 | -8.615850e-47 |
| Msa0735340 | Msa1402000 | 0.826715 | 2.329644e-54 | -8.615850e-47 |
| Msa0747500 | Msa1402000 | 0.836575 | 8.634672e-57 | -8.615850e-47 |
| Msa0757990 | Msa1402000 | 0.803005 | 4.285869e-49 | -8.615850e-47 |
| Msa0758000 | Msa1402000 | 0.802193 | 6.299708e-49 | -8.615850e-47 |
| Msa0777210 | Msa1402000 | 0.811928 | 5.476125e-51 | -8.615850e-47 |
| Msa0795330 | Msa1402000 | 0.809298 | 2.027588e-50 | -8.615850e-47 |
| Msa0814350 | Msa1402000 | 0.826927 | 2.073578e-54 | -8.615850e-47 |
| Msa0830830 | Msa1402000 | -0.814618 | 1.405613e-51 | -8.615850e-47 |
| Msa0128340 | Msa1402000 | 0.819624 | 1.053347e-52 | -8.615850e-47 |
| Msa0128740 | Msa1402000 | 0.818539 | 1.859511e-52 | -8.615850e-47 |
| Msa0129270 | Msa1402000 | 0.833317 | 5.716044e-56 | -8.615850e-47 |
| Msa0131800 | Msa1402000 | 0.889292 | 2.759814e-73 | -8.615850e-47 |
| Msa0137970 | Msa1402000 | 0.811838 | 5.731145e-51 | -8.615850e-47 |
| Msa0154750 | Msa1402000 | 0.810694 | 1.014923e-50 | -8.615850e-47 |
| Msa0173260 | Msa1402000 | 0.944348 | 2.298185e-103 | -8.615850e-47 |
| Msa0192890 | Msa1402000 | 0.814658 | 1.377027e-51 | -8.615850e-47 |
| Msa0223050 | Msa1402000 | 0.842908 | 1.939906e-58 | -8.615850e-47 |
| Msa0235020 | Msa1402000 | 0.806526 | 7.881657e-50 | -8.615850e-47 |
| Msa0238420 | Msa1402000 | 0.800908 | 1.155959e-48 | -8.615850e-47 |
| Msa1251500 | Msa1402000 | 0.805858 | 1.089760e-49 | -8.615850e-47 |
| Msa1257170 | Msa1402000 | 0.828704 | 7.754515e-55 | -8.615850e-47 |
| Msa1258600 | Msa1402000 | 0.835347 | 1.768811e-56 | -8.615850e-47 |
| Msa1265500 | Msa1402000 | 0.820397 | 7.008246e-53 | -8.615850e-47 |
| Msa1266710 | Msa1402000 | 0.812922 | 3.322894e-51 | -8.615850e-47 |
| Msa1298440 | Msa1402000 | 0.830473 | 2.878889e-55 | -8.615850e-47 |
| Msa1302120 | Msa1402000 | 0.825797 | 3.853483e-54 | -8.615850e-47 |
| Msa1335500 | Msa1402000 | 0.814675 | 1.365579e-51 | -8.615850e-47 |
| Msa0375500 | Msa1402000 | 0.833000 | 6.856627e-56 | -8.615850e-47 |
| Msa0375550 | Msa1402000 | 0.846924 | 1.599429e-59 | -8.615850e-47 |
| Msa0384220 | Msa1402000 | 0.822326 | 2.515454e-53 | -8.615850e-47 |
| Msa0421100 | Msa1402000 | -0.803308 | 3.708835e-49 | -8.615850e-47 |
| Msa0424240 | Msa1402000 | 0.830258 | 3.249865e-55 | -8.615850e-47 |
| Msa0424300 | Msa1402000 | 0.835743 | 1.404900e-56 | -8.615850e-47 |
| Msa0427850 | Msa1402000 | 0.815309 | 9.873557e-52 | -8.615850e-47 |
| Msa0434890 | Msa1402000 | 0.843653 | 1.227898e-58 | -8.615850e-47 |
| Msa0465420 | Msa1402000 | 0.811425 | 7.047891e-51 | -8.615850e-47 |
| Msa0471210 | Msa1402000 | 0.847586 | 1.053299e-59 | -8.615850e-47 |
| Msa0474080 | Msa1402000 | 0.815624 | 8.402516e-52 | -8.615850e-47 |
| Msa0476820 | Msa1402000 | -0.804755 | 1.854844e-49 | -8.615850e-47 |
| Msa0478400 | Msa1402000 | 0.830762 | 2.446886e-55 | -8.615850e-47 |
| Msa0482360 | Msa1402000 | 0.818493 | 1.904316e-52 | -8.615850e-47 |
| Msa0980490 | Msa1402000 | 0.802741 | 4.859421e-49 | -8.615850e-47 |
| Msa0996940 | Msa1402000 | 0.825107 | 5.614793e-54 | -8.615850e-47 |
| Msa1046220 | Msa1402000 | 0.831313 | 1.791688e-55 | -8.615850e-47 |
| Msa0261490 | Msa1402000 | 0.821608 | 3.688232e-53 | -8.615850e-47 |
| Msa0264220 | Msa1402000 | 0.815754 | 7.861471e-52 | -8.615850e-47 |
| Msa0273420 | Msa1402000 | 0.807570 | 4.738685e-50 | -8.615850e-47 |
| Msa0275570 | Msa1402000 | 0.807374 | 5.215513e-50 | -8.615850e-47 |
| Msa0302220 | Msa1402000 | 0.826219 | 3.059090e-54 | -8.615850e-47 |
| Msa0311130 | Msa1402000 | -0.802134 | 6.480186e-49 | -8.615850e-47 |
| Msa0313680 | Msa1402000 | 0.807709 | 4.428300e-50 | -8.615850e-47 |
| Msa0339540 | Msa1402000 | 0.823386 | 1.424140e-53 | -8.615850e-47 |
| Msa1171630 | Msa1402000 | 0.834464 | 2.953089e-56 | -8.615850e-47 |
| Msa1171720 | Msa1402000 | 0.800054 | 1.725530e-48 | -8.615850e-47 |
| Msa1207000 | Msa1402000 | 0.825990 | 3.468136e-54 | -8.615850e-47 |
| Msa1207090 | Msa1402000 | 0.821041 | 4.984261e-53 | -8.615850e-47 |
| Msa1219780 | Msa1402000 | 0.807026 | 6.179744e-50 | -8.615850e-47 |
| Msa1221170 | Msa1402000 | 0.824656 | 7.170761e-54 | -8.615850e-47 |
| Msa0511280 | Msa1402000 | 0.803106 | 4.082844e-49 | -8.615850e-47 |
| Msa0515930 | Msa1402000 | 0.836606 | 8.481801e-57 | -8.615850e-47 |
| Msa0854860 | Msa1402000 | 0.810110 | 1.356655e-50 | -8.615850e-47 |
| Msa0878410 | Msa1402000 | 0.819673 | 1.026544e-52 | -8.615850e-47 |
| Msa0896990 | Msa1402000 | 0.830168 | 3.418004e-55 | -8.615850e-47 |
| Msa0904940 | Msa1402000 | 0.800776 | 1.229515e-48 | -8.615850e-47 |
| Msa0911490 | Msa1402000 | -0.804563 | 2.034572e-49 | -8.615850e-47 |
| Msa0931370 | Msa1402000 | 0.806969 | 6.353935e-50 | -8.615850e-47 |
| Msa0941520 | Msa1402000 | 0.822527 | 2.258790e-53 | -8.615850e-47 |
| Msa0962260 | Msa1402000 | 0.820108 | 8.165173e-53 | -8.615850e-47 |
| Msa0978530 | Msa1402000 | 0.823946 | 1.053377e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1402000 | MtrunA17_Chr1g0192651 | 100.000 | 165 | 0 | 0 | 1 | 165 | 1 | 165 | 2.14e-123 | 343 |
| Msa1402000 | MtrunA17_Chr1g0194801 | 53.247 | 154 | 66 | 3 | 9 | 157 | 6 | 158 | 3.20e-56 | 174 |
| Msa1402000 | MtrunA17_Chr1g0176311 | 40.741 | 162 | 83 | 3 | 10 | 158 | 23 | 184 | 1.98e-34 | 119 |
| Msa1402000 | MtrunA17_Chr1g0176321 | 38.690 | 168 | 84 | 4 | 10 | 158 | 4 | 171 | 8.67e-33 | 114 |
| Msa1402000 | MtrunA17_Chr1g0195661 | 40.132 | 152 | 88 | 2 | 11 | 159 | 14 | 165 | 2.24e-32 | 113 |
| Msa1402000 | MtrunA17_Chr5g0427291 | 37.037 | 162 | 84 | 5 | 11 | 155 | 10 | 170 | 3.03e-30 | 108 |
| Msa1402000 | MtrunA17_Chr7g0269301 | 40.123 | 162 | 90 | 4 | 1 | 158 | 1 | 159 | 3.91e-27 | 100 |
| Msa1402000 | MtrunA17_Chr2g0323291 | 30.539 | 167 | 102 | 4 | 9 | 163 | 25 | 189 | 1.09e-21 | 87.4 |
| Msa1402000 | MtrunA17_Chr5g0433951 | 33.962 | 159 | 89 | 5 | 13 | 160 | 9 | 162 | 6.64e-19 | 79.0 |
| Msa1402000 | MtrunA17_Chr4g0001641 | 52.381 | 63 | 30 | 0 | 93 | 155 | 3 | 65 | 1.65e-18 | 75.1 |
| Msa1402000 | MtrunA17_Chr2g0294421 | 31.250 | 160 | 94 | 4 | 7 | 155 | 3 | 157 | 1.22e-17 | 75.5 |
| Msa1402000 | MtrunA17_Chr6g0450171 | 27.485 | 171 | 110 | 5 | 6 | 164 | 3 | 171 | 3.27e-17 | 74.7 |
| Msa1402000 | MtrunA17_Chr4g0008801 | 34.194 | 155 | 92 | 4 | 9 | 155 | 5 | 157 | 1.69e-16 | 72.4 |
| Msa1402000 | MtrunA17_Chr4g0032261 | 31.902 | 163 | 101 | 4 | 7 | 161 | 3 | 163 | 3.74e-14 | 66.2 |
| Msa1402000 | MtrunA17_Chr7g0240701 | 31.613 | 155 | 96 | 4 | 9 | 155 | 5 | 157 | 1.25e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1402000 | AT3G62550.1 | 50.993 | 151 | 71 | 1 | 9 | 156 | 7 | 157 | 5.83e-52 | 163 |
| Msa1402000 | AT3G11930.2 | 38.922 | 167 | 88 | 4 | 9 | 161 | 33 | 199 | 1.87e-34 | 120 |
| Msa1402000 | AT3G11930.1 | 39.157 | 166 | 88 | 4 | 9 | 161 | 33 | 198 | 2.11e-34 | 120 |
| Msa1402000 | AT3G11930.4 | 38.690 | 168 | 88 | 5 | 9 | 161 | 33 | 200 | 1.02e-33 | 118 |
| Msa1402000 | AT3G58450.1 | 38.650 | 163 | 88 | 4 | 11 | 161 | 32 | 194 | 7.92e-32 | 113 |
| Msa1402000 | AT3G58450.2 | 39.375 | 160 | 84 | 3 | 11 | 161 | 32 | 187 | 4.05e-31 | 111 |
| Msa1402000 | AT1G09740.2 | 37.654 | 162 | 91 | 3 | 11 | 162 | 10 | 171 | 1.25e-30 | 109 |
| Msa1402000 | AT1G09740.1 | 37.654 | 162 | 91 | 3 | 11 | 162 | 10 | 171 | 1.25e-30 | 109 |
| Msa1402000 | AT2G47710.1 | 36.875 | 160 | 92 | 4 | 5 | 158 | 3 | 159 | 6.59e-30 | 107 |
| Msa1402000 | AT3G11930.3 | 33.679 | 193 | 88 | 5 | 9 | 161 | 33 | 225 | 3.62e-28 | 104 |
| Msa1402000 | AT1G68300.1 | 36.242 | 149 | 92 | 3 | 9 | 155 | 10 | 157 | 5.46e-27 | 99.8 |
| Msa1402000 | AT3G17020.1 | 36.025 | 161 | 84 | 6 | 8 | 155 | 6 | 160 | 3.01e-20 | 82.4 |
| Msa1402000 | AT1G11360.2 | 29.586 | 169 | 104 | 5 | 9 | 165 | 38 | 203 | 2.38e-18 | 79.3 |
| Msa1402000 | AT1G11360.1 | 29.586 | 169 | 104 | 5 | 9 | 165 | 38 | 203 | 2.38e-18 | 79.3 |
| Msa1402000 | AT1G11360.4 | 29.586 | 169 | 104 | 5 | 9 | 165 | 38 | 203 | 2.38e-18 | 79.3 |
| Msa1402000 | AT1G11360.3 | 29.586 | 169 | 104 | 5 | 9 | 165 | 38 | 203 | 2.38e-18 | 79.3 |
| Msa1402000 | AT3G01520.1 | 31.944 | 144 | 93 | 4 | 24 | 164 | 30 | 171 | 1.57e-17 | 75.9 |
| Msa1402000 | AT5G14680.1 | 29.940 | 167 | 103 | 5 | 10 | 164 | 7 | 171 | 8.98e-17 | 73.9 |
| Msa1402000 | AT3G03270.2 | 30.818 | 159 | 94 | 5 | 8 | 155 | 4 | 157 | 2.04e-15 | 69.7 |
| Msa1402000 | AT3G53990.1 | 28.302 | 159 | 96 | 4 | 9 | 155 | 5 | 157 | 3.19e-12 | 61.6 |
| Msa1402000 | AT3G25930.1 | 29.012 | 162 | 87 | 8 | 9 | 156 | 2 | 149 | 3.24e-11 | 58.5 |
Find 55 sgRNAs with CRISPR-Local
Find 148 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCCTCCAGTGGTTGATTT+TGG | 0.145041 | tig0022800:-28434 | None:intergenic |
| CACATGGTGCCTCAAGAATT+TGG | 0.151045 | tig0022800:+27738 | Msa1402000:CDS |
| ATATATTGTGCTTCAAATTA+TGG | 0.208500 | tig0022800:-27541 | None:intergenic |
| TTTCAAATATTAAATGATAA+AGG | 0.289136 | tig0022800:+28594 | Msa1402000:three_prime_UTR |
| GTTGTTTATTCTGCCTTTGA+TGG | 0.302678 | tig0022800:+27814 | Msa1402000:CDS |
| TTTCACACTGCTTGATAATT+CGG | 0.344578 | tig0022800:-27642 | None:intergenic |
| GGCTTCTTCACAATCAAAAC+AGG | 0.346785 | tig0022800:-28413 | None:intergenic |
| ACAAGTTGCTGATTGTGTTT+TGG | 0.353665 | tig0022800:+27974 | Msa1402000:CDS |
| GGGTGTTGATATCTTGGTTA+TGG | 0.361677 | tig0022800:+28194 | Msa1402000:CDS |
| GAGGAGGATAAGGTGATCTT+TGG | 0.363644 | tig0022800:-27773 | None:intergenic |
| TTGCTATGAACATGTCCTTT+GGG | 0.364433 | tig0022800:+28535 | Msa1402000:three_prime_UTR |
| ATTGCTATGAACATGTCCTT+TGG | 0.383983 | tig0022800:+28534 | Msa1402000:three_prime_UTR |
| GCGTGTCTTGTTCCTTCATA+AGG | 0.398759 | tig0022800:+28562 | Msa1402000:three_prime_UTR |
| AAAAGATATATTCCTTATGA+AGG | 0.407539 | tig0022800:-28574 | None:intergenic |
| GACATCATTACAGACTATCT+TGG | 0.412460 | tig0022800:-28003 | None:intergenic |
| GGTGTTGATATCTTGGTTAT+GGG | 0.433422 | tig0022800:+28195 | Msa1402000:CDS |
| AAACACAATCAGCAACTTGT+TGG | 0.448947 | tig0022800:-27971 | None:intergenic |
| CCGTTATGTCAGAAGAGAAT+AGG | 0.454640 | tig0022800:-27929 | None:intergenic |
| CCCTAGGGATGTAATCTGTC+AGG | 0.458933 | tig0022800:+28158 | Msa1402000:CDS |
| TATTCTGCCTTTGATGGCAC+AGG | 0.479279 | tig0022800:+27820 | Msa1402000:CDS |
| TTGAATGGCAGGGCTTTCCT+TGG | 0.488782 | tig0022800:+28353 | Msa1402000:intron |
| ATCTTAATTGTCTCCTCCAG+TGG | 0.492223 | tig0022800:-28444 | None:intergenic |
| TTGTTGGCTATACTTCTCCA+TGG | 0.495342 | tig0022800:-27955 | None:intergenic |
| GCTTAACATAGAGGAGGATA+AGG | 0.496830 | tig0022800:-27783 | None:intergenic |
| ATGGCTGGTATAACAGAAAA+CGG | 0.497259 | tig0022800:+27664 | Msa1402000:CDS |
| AATTATCAAGCAGTGTGAAA+TGG | 0.497338 | tig0022800:+27645 | Msa1402000:exon |
| GTGGAGACGAGGATAGAGAA+TGG | 0.517274 | tig0022800:+28132 | Msa1402000:CDS |
| GTTATGGGAAGCCACGGCTA+TGG | 0.518096 | tig0022800:+28210 | Msa1402000:CDS |
| GGATAGAGAATGGTGACCCT+AGG | 0.527492 | tig0022800:+28142 | Msa1402000:CDS |
| CCTATTCTCTTCTGACATAA+CGG | 0.542181 | tig0022800:+27929 | Msa1402000:CDS |
| ATCAAGCAGTGTGAAATGGC+TGG | 0.546216 | tig0022800:+27649 | Msa1402000:exon |
| ATCTTGGTTATGGGAAGCCA+CGG | 0.562584 | tig0022800:+28204 | Msa1402000:CDS |
| AGAAAACGGAAGAAGAATCA+TGG | 0.569970 | tig0022800:+27678 | Msa1402000:CDS |
| CACGAGGAGGCTTAACATAG+AGG | 0.577003 | tig0022800:-27792 | None:intergenic |
| AGTGGTTGCTCACACTTCCA+AGG | 0.579050 | tig0022800:-28370 | None:intergenic |
| TTAATTACCTGTGCCATCAA+AGG | 0.579397 | tig0022800:-27827 | None:intergenic |
| GATAGAGAATGGTGACCCTA+GGG | 0.580628 | tig0022800:+28143 | Msa1402000:CDS |
| AAGAAGCCAAAATCAACCAC+TGG | 0.584975 | tig0022800:+28428 | Msa1402000:CDS |
| GCCTGACAGATTACATCCCT+AGG | 0.585000 | tig0022800:-28159 | None:intergenic |
| ATCATGGTAGCTATTGATGA+AGG | 0.586206 | tig0022800:+27694 | Msa1402000:CDS |
| TTCTGACATAACGGCAACCA+TGG | 0.586507 | tig0022800:+27938 | Msa1402000:CDS |
| ACAAGACACGCACAACCCAA+AGG | 0.602143 | tig0022800:-28550 | None:intergenic |
| ATTGTGCTTCAAATTATGGT+CGG | 0.617713 | tig0022800:-27537 | None:intergenic |
| TCGGTTTGAGATAGATCACA+AGG | 0.631463 | tig0022800:-27623 | None:intergenic |
| ACGGCTATGGTGTCATCAAG+AGG | 0.638531 | tig0022800:+28223 | Msa1402000:CDS |
| AAGCCAAAATCAACCACTGG+AGG | 0.644827 | tig0022800:+28431 | Msa1402000:CDS |
| GCTTCTTCACAATCAAAACA+GGG | 0.652447 | tig0022800:-28412 | None:intergenic |
| ATATGGTTTAGGTGGAGACG+AGG | 0.658827 | tig0022800:+28121 | Msa1402000:intron |
| CCTGACAGATTACATCCCTA+GGG | 0.659449 | tig0022800:-28158 | None:intergenic |
| AGAGCATTTATGCTCTCACA+TGG | 0.669047 | tig0022800:+27722 | Msa1402000:CDS |
| TCTTGATGACACCATAGCCG+TGG | 0.679887 | tig0022800:-28221 | None:intergenic |
| ACTTCACATTCTGTGCACAG+TGG | 0.689187 | tig0022800:-28388 | None:intergenic |
| GCAGAATAAACAACACGAGG+AGG | 0.692643 | tig0022800:-27805 | None:intergenic |
| GAGGAGGCTTAACATAGAGG+AGG | 0.704073 | tig0022800:-27789 | None:intergenic |
| AAGGCAGAATAAACAACACG+AGG | 0.733599 | tig0022800:-27808 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTCAAATATTAAATGATAA+AGG | + | tig0022800:28594-28613 | Msa1402000:three_prime_UTR | 10.0% |
| !! | TTTCAAATATTAAATGATAA+AGG | + | tig0022800:28594-28613 | Msa1402000:three_prime_UTR | 10.0% |
| !!! | ATATATGTATACTTACATTT+TGG | - | tig0022800:28028-28047 | None:intergenic | 15.0% |
| !! | ATTAACTATAGATAAAAATG+AGG | - | tig0022800:28081-28100 | None:intergenic | 15.0% |
| !! | TTAATAATTAATCTGTGATA+TGG | + | tig0022800:28104-28123 | Msa1402000:intron | 15.0% |
| !! | TGTGAATTGTAAATTTAATT+TGG | + | tig0022800:28315-28334 | Msa1402000:intron | 15.0% |
| !! | GAAAAGAAAAAAACAAAATA+AGG | - | tig0022800:28485-28504 | None:intergenic | 15.0% |
| !!! | ATATATGTATACTTACATTT+TGG | - | tig0022800:28028-28047 | None:intergenic | 15.0% |
| !! | ATTAACTATAGATAAAAATG+AGG | - | tig0022800:28081-28100 | None:intergenic | 15.0% |
| !! | TTAATAATTAATCTGTGATA+TGG | + | tig0022800:28104-28123 | Msa1402000:intron | 15.0% |
| !! | TGTGAATTGTAAATTTAATT+TGG | + | tig0022800:28315-28334 | Msa1402000:intron | 15.0% |
| !! | GAAAAGAAAAAAACAAAATA+AGG | - | tig0022800:28485-28504 | None:intergenic | 15.0% |
| !! | ATATATTGTGCTTCAAATTA+TGG | - | tig0022800:27544-27563 | None:intergenic | 20.0% |
| !!! | TTGTAAATTTAATTTGGTTC+TGG | + | tig0022800:28321-28340 | Msa1402000:intron | 20.0% |
| !! | AAAAGATATATTCCTTATGA+AGG | - | tig0022800:28577-28596 | None:intergenic | 20.0% |
| !! | ATATATTGTGCTTCAAATTA+TGG | - | tig0022800:27544-27563 | None:intergenic | 20.0% |
| !!! | TTGTAAATTTAATTTGGTTC+TGG | + | tig0022800:28321-28340 | Msa1402000:intron | 20.0% |
| !! | AAAAGATATATTCCTTATGA+AGG | - | tig0022800:28577-28596 | None:intergenic | 20.0% |
| ! | TTTGAAAAACCAAATTCTTG+AGG | - | tig0022800:27750-27769 | None:intergenic | 25.0% |
| ! | GTATCATATATACATGTTTC+AGG | + | tig0022800:27903-27922 | Msa1402000:intron | 25.0% |
| ! | ACTATAGATAAAAATGAGGA+GGG | - | tig0022800:28077-28096 | None:intergenic | 25.0% |
| ! | AACTATAGATAAAAATGAGG+AGG | - | tig0022800:28078-28097 | None:intergenic | 25.0% |
| ! | ATTAATCTGTGATATGGTTT+AGG | + | tig0022800:28110-28129 | Msa1402000:intron | 25.0% |
| !! | ATTTTTGTTAGAATGAGCTA+AGG | + | tig0022800:28291-28310 | Msa1402000:intron | 25.0% |
| ! | TTTGAAAAACCAAATTCTTG+AGG | - | tig0022800:27750-27769 | None:intergenic | 25.0% |
| ! | GTATCATATATACATGTTTC+AGG | + | tig0022800:27903-27922 | Msa1402000:intron | 25.0% |
| ! | ACTATAGATAAAAATGAGGA+GGG | - | tig0022800:28077-28096 | None:intergenic | 25.0% |
| ! | AACTATAGATAAAAATGAGG+AGG | - | tig0022800:28078-28097 | None:intergenic | 25.0% |
| ! | ATTAATCTGTGATATGGTTT+AGG | + | tig0022800:28110-28129 | Msa1402000:intron | 25.0% |
| !! | ATTTTTGTTAGAATGAGCTA+AGG | + | tig0022800:28291-28310 | Msa1402000:intron | 25.0% |
| ATTGTGCTTCAAATTATGGT+CGG | - | tig0022800:27540-27559 | None:intergenic | 30.0% | |
| ! | TTTCACACTGCTTGATAATT+CGG | - | tig0022800:27645-27664 | None:intergenic | 30.0% |
| AATTATCAAGCAGTGTGAAA+TGG | + | tig0022800:27645-27664 | Msa1402000:exon | 30.0% | |
| !! | AAAAATGGGTGTTGATATCT+TGG | + | tig0022800:28188-28207 | Msa1402000:CDS | 30.0% |
| ATTGTGCTTCAAATTATGGT+CGG | - | tig0022800:27540-27559 | None:intergenic | 30.0% | |
| ! | TTTCACACTGCTTGATAATT+CGG | - | tig0022800:27645-27664 | None:intergenic | 30.0% |
| AATTATCAAGCAGTGTGAAA+TGG | + | tig0022800:27645-27664 | Msa1402000:exon | 30.0% | |
| !! | AAAAATGGGTGTTGATATCT+TGG | + | tig0022800:28188-28207 | Msa1402000:CDS | 30.0% |
| ATGGCTGGTATAACAGAAAA+CGG | + | tig0022800:27664-27683 | Msa1402000:CDS | 35.0% | |
| AGAAAACGGAAGAAGAATCA+TGG | + | tig0022800:27678-27697 | Msa1402000:CDS | 35.0% | |
| ATCATGGTAGCTATTGATGA+AGG | + | tig0022800:27694-27713 | Msa1402000:CDS | 35.0% | |
| GTTGTTTATTCTGCCTTTGA+TGG | + | tig0022800:27814-27833 | Msa1402000:CDS | 35.0% | |
| TTAATTACCTGTGCCATCAA+AGG | - | tig0022800:27830-27849 | None:intergenic | 35.0% | |
| CCTATTCTCTTCTGACATAA+CGG | + | tig0022800:27929-27948 | Msa1402000:CDS | 35.0% | |
| AAACACAATCAGCAACTTGT+TGG | - | tig0022800:27974-27993 | None:intergenic | 35.0% | |
| !! | ACAAGTTGCTGATTGTGTTT+TGG | + | tig0022800:27974-27993 | Msa1402000:CDS | 35.0% |
| GACATCATTACAGACTATCT+TGG | - | tig0022800:28006-28025 | None:intergenic | 35.0% | |
| AATCTGTGATATGGTTTAGG+TGG | + | tig0022800:28113-28132 | Msa1402000:intron | 35.0% | |
| TGTCAGGCTGTTCAAAAAAT+GGG | + | tig0022800:28174-28193 | Msa1402000:CDS | 35.0% | |
| !! | GGTGTTGATATCTTGGTTAT+GGG | + | tig0022800:28195-28214 | Msa1402000:CDS | 35.0% |
| ! | TTCTGGCTGTTATTGTTGAA+TGG | + | tig0022800:28338-28357 | Msa1402000:intron | 35.0% |
| GCTTCTTCACAATCAAAACA+GGG | - | tig0022800:28415-28434 | None:intergenic | 35.0% | |
| ATTGCTATGAACATGTCCTT+TGG | + | tig0022800:28534-28553 | Msa1402000:three_prime_UTR | 35.0% | |
| TTGCTATGAACATGTCCTTT+GGG | + | tig0022800:28535-28554 | Msa1402000:three_prime_UTR | 35.0% | |
| ATGGCTGGTATAACAGAAAA+CGG | + | tig0022800:27664-27683 | Msa1402000:CDS | 35.0% | |
| AGAAAACGGAAGAAGAATCA+TGG | + | tig0022800:27678-27697 | Msa1402000:CDS | 35.0% | |
| ATCATGGTAGCTATTGATGA+AGG | + | tig0022800:27694-27713 | Msa1402000:CDS | 35.0% | |
| GTTGTTTATTCTGCCTTTGA+TGG | + | tig0022800:27814-27833 | Msa1402000:CDS | 35.0% | |
| TTAATTACCTGTGCCATCAA+AGG | - | tig0022800:27830-27849 | None:intergenic | 35.0% | |
| CCTATTCTCTTCTGACATAA+CGG | + | tig0022800:27929-27948 | Msa1402000:CDS | 35.0% | |
| AAACACAATCAGCAACTTGT+TGG | - | tig0022800:27974-27993 | None:intergenic | 35.0% | |
| !! | ACAAGTTGCTGATTGTGTTT+TGG | + | tig0022800:27974-27993 | Msa1402000:CDS | 35.0% |
| GACATCATTACAGACTATCT+TGG | - | tig0022800:28006-28025 | None:intergenic | 35.0% | |
| AATCTGTGATATGGTTTAGG+TGG | + | tig0022800:28113-28132 | Msa1402000:intron | 35.0% | |
| TGTCAGGCTGTTCAAAAAAT+GGG | + | tig0022800:28174-28193 | Msa1402000:CDS | 35.0% | |
| !! | GGTGTTGATATCTTGGTTAT+GGG | + | tig0022800:28195-28214 | Msa1402000:CDS | 35.0% |
| ! | TTCTGGCTGTTATTGTTGAA+TGG | + | tig0022800:28338-28357 | Msa1402000:intron | 35.0% |
| GCTTCTTCACAATCAAAACA+GGG | - | tig0022800:28415-28434 | None:intergenic | 35.0% | |
| ATTGCTATGAACATGTCCTT+TGG | + | tig0022800:28534-28553 | Msa1402000:three_prime_UTR | 35.0% | |
| TTGCTATGAACATGTCCTTT+GGG | + | tig0022800:28535-28554 | Msa1402000:three_prime_UTR | 35.0% | |
| ! | TCGGTTTGAGATAGATCACA+AGG | - | tig0022800:27626-27645 | None:intergenic | 40.0% |
| ! | AGAGCATTTATGCTCTCACA+TGG | + | tig0022800:27722-27741 | Msa1402000:CDS | 40.0% |
| GCTTAACATAGAGGAGGATA+AGG | - | tig0022800:27786-27805 | None:intergenic | 40.0% | |
| AAGGCAGAATAAACAACACG+AGG | - | tig0022800:27811-27830 | None:intergenic | 40.0% | |
| CCGTTATGTCAGAAGAGAAT+AGG | - | tig0022800:27932-27951 | None:intergenic | 40.0% | |
| ! | TTGTTGGCTATACTTCTCCA+TGG | - | tig0022800:27958-27977 | None:intergenic | 40.0% |
| CTGTCAGGCTGTTCAAAAAA+TGG | + | tig0022800:28173-28192 | Msa1402000:CDS | 40.0% | |
| !! | GGGTGTTGATATCTTGGTTA+TGG | + | tig0022800:28194-28213 | Msa1402000:CDS | 40.0% |
| GCTGTTATTGTTGAATGGCA+GGG | + | tig0022800:28343-28362 | Msa1402000:intron | 40.0% | |
| GGCTTCTTCACAATCAAAAC+AGG | - | tig0022800:28416-28435 | None:intergenic | 40.0% | |
| AAGAAGCCAAAATCAACCAC+TGG | + | tig0022800:28428-28447 | Msa1402000:CDS | 40.0% | |
| ATCTTAATTGTCTCCTCCAG+TGG | - | tig0022800:28447-28466 | None:intergenic | 40.0% | |
| ! | TCGGTTTGAGATAGATCACA+AGG | - | tig0022800:27626-27645 | None:intergenic | 40.0% |
| ! | AGAGCATTTATGCTCTCACA+TGG | + | tig0022800:27722-27741 | Msa1402000:CDS | 40.0% |
| GCTTAACATAGAGGAGGATA+AGG | - | tig0022800:27786-27805 | None:intergenic | 40.0% | |
| AAGGCAGAATAAACAACACG+AGG | - | tig0022800:27811-27830 | None:intergenic | 40.0% | |
| CCGTTATGTCAGAAGAGAAT+AGG | - | tig0022800:27932-27951 | None:intergenic | 40.0% | |
| ! | TTGTTGGCTATACTTCTCCA+TGG | - | tig0022800:27958-27977 | None:intergenic | 40.0% |
| CTGTCAGGCTGTTCAAAAAA+TGG | + | tig0022800:28173-28192 | Msa1402000:CDS | 40.0% | |
| !! | GGGTGTTGATATCTTGGTTA+TGG | + | tig0022800:28194-28213 | Msa1402000:CDS | 40.0% |
| GCTGTTATTGTTGAATGGCA+GGG | + | tig0022800:28343-28362 | Msa1402000:intron | 40.0% | |
| GGCTTCTTCACAATCAAAAC+AGG | - | tig0022800:28416-28435 | None:intergenic | 40.0% | |
| AAGAAGCCAAAATCAACCAC+TGG | + | tig0022800:28428-28447 | Msa1402000:CDS | 40.0% | |
| ATCTTAATTGTCTCCTCCAG+TGG | - | tig0022800:28447-28466 | None:intergenic | 40.0% | |
| ATCAAGCAGTGTGAAATGGC+TGG | + | tig0022800:27649-27668 | Msa1402000:exon | 45.0% | |
| CACATGGTGCCTCAAGAATT+TGG | + | tig0022800:27738-27757 | Msa1402000:CDS | 45.0% | |
| GAGGAGGATAAGGTGATCTT+TGG | - | tig0022800:27776-27795 | None:intergenic | 45.0% | |
| GCAGAATAAACAACACGAGG+AGG | - | tig0022800:27808-27827 | None:intergenic | 45.0% | |
| ! | TATTCTGCCTTTGATGGCAC+AGG | + | tig0022800:27820-27839 | Msa1402000:CDS | 45.0% |
| TTCTGACATAACGGCAACCA+TGG | + | tig0022800:27938-27957 | Msa1402000:CDS | 45.0% | |
| ATATGGTTTAGGTGGAGACG+AGG | + | tig0022800:28121-28140 | Msa1402000:intron | 45.0% | |
| GATAGAGAATGGTGACCCTA+GGG | + | tig0022800:28143-28162 | Msa1402000:CDS | 45.0% | |
| CCTGACAGATTACATCCCTA+GGG | - | tig0022800:28161-28180 | None:intergenic | 45.0% | |
| ATCTTGGTTATGGGAAGCCA+CGG | + | tig0022800:28204-28223 | Msa1402000:CDS | 45.0% | |
| GGCTGTTATTGTTGAATGGC+AGG | + | tig0022800:28342-28361 | Msa1402000:intron | 45.0% | |
| ACTTCACATTCTGTGCACAG+TGG | - | tig0022800:28391-28410 | None:intergenic | 45.0% | |
| AAGCCAAAATCAACCACTGG+AGG | + | tig0022800:28431-28450 | Msa1402000:CDS | 45.0% | |
| !! | TCTCCTCCAGTGGTTGATTT+TGG | - | tig0022800:28437-28456 | None:intergenic | 45.0% |
| ! | GCGTGTCTTGTTCCTTCATA+AGG | + | tig0022800:28562-28581 | Msa1402000:three_prime_UTR | 45.0% |
| ATCAAGCAGTGTGAAATGGC+TGG | + | tig0022800:27649-27668 | Msa1402000:exon | 45.0% | |
| CACATGGTGCCTCAAGAATT+TGG | + | tig0022800:27738-27757 | Msa1402000:CDS | 45.0% | |
| GAGGAGGATAAGGTGATCTT+TGG | - | tig0022800:27776-27795 | None:intergenic | 45.0% | |
| GCAGAATAAACAACACGAGG+AGG | - | tig0022800:27808-27827 | None:intergenic | 45.0% | |
| ! | TATTCTGCCTTTGATGGCAC+AGG | + | tig0022800:27820-27839 | Msa1402000:CDS | 45.0% |
| TTCTGACATAACGGCAACCA+TGG | + | tig0022800:27938-27957 | Msa1402000:CDS | 45.0% | |
| ATATGGTTTAGGTGGAGACG+AGG | + | tig0022800:28121-28140 | Msa1402000:intron | 45.0% | |
| GATAGAGAATGGTGACCCTA+GGG | + | tig0022800:28143-28162 | Msa1402000:CDS | 45.0% | |
| CCTGACAGATTACATCCCTA+GGG | - | tig0022800:28161-28180 | None:intergenic | 45.0% | |
| ATCTTGGTTATGGGAAGCCA+CGG | + | tig0022800:28204-28223 | Msa1402000:CDS | 45.0% | |
| GGCTGTTATTGTTGAATGGC+AGG | + | tig0022800:28342-28361 | Msa1402000:intron | 45.0% | |
| ACTTCACATTCTGTGCACAG+TGG | - | tig0022800:28391-28410 | None:intergenic | 45.0% | |
| AAGCCAAAATCAACCACTGG+AGG | + | tig0022800:28431-28450 | Msa1402000:CDS | 45.0% | |
| !! | TCTCCTCCAGTGGTTGATTT+TGG | - | tig0022800:28437-28456 | None:intergenic | 45.0% |
| ! | GCGTGTCTTGTTCCTTCATA+AGG | + | tig0022800:28562-28581 | Msa1402000:three_prime_UTR | 45.0% |
| GAGGAGGCTTAACATAGAGG+AGG | - | tig0022800:27792-27811 | None:intergenic | 50.0% | |
| CACGAGGAGGCTTAACATAG+AGG | - | tig0022800:27795-27814 | None:intergenic | 50.0% | |
| GTGGAGACGAGGATAGAGAA+TGG | + | tig0022800:28132-28151 | Msa1402000:CDS | 50.0% | |
| GGATAGAGAATGGTGACCCT+AGG | + | tig0022800:28142-28161 | Msa1402000:CDS | 50.0% | |
| CCCTAGGGATGTAATCTGTC+AGG | + | tig0022800:28158-28177 | Msa1402000:CDS | 50.0% | |
| GCCTGACAGATTACATCCCT+AGG | - | tig0022800:28162-28181 | None:intergenic | 50.0% | |
| ! | TCTTGATGACACCATAGCCG+TGG | - | tig0022800:28224-28243 | None:intergenic | 50.0% |
| !! | ACGGCTATGGTGTCATCAAG+AGG | + | tig0022800:28223-28242 | Msa1402000:CDS | 50.0% |
| TTGAATGGCAGGGCTTTCCT+TGG | + | tig0022800:28353-28372 | Msa1402000:intron | 50.0% | |
| AGTGGTTGCTCACACTTCCA+AGG | - | tig0022800:28373-28392 | None:intergenic | 50.0% | |
| ACAAGACACGCACAACCCAA+AGG | - | tig0022800:28553-28572 | None:intergenic | 50.0% | |
| GAGGAGGCTTAACATAGAGG+AGG | - | tig0022800:27792-27811 | None:intergenic | 50.0% | |
| CACGAGGAGGCTTAACATAG+AGG | - | tig0022800:27795-27814 | None:intergenic | 50.0% | |
| GTGGAGACGAGGATAGAGAA+TGG | + | tig0022800:28132-28151 | Msa1402000:CDS | 50.0% | |
| GGATAGAGAATGGTGACCCT+AGG | + | tig0022800:28142-28161 | Msa1402000:CDS | 50.0% | |
| CCCTAGGGATGTAATCTGTC+AGG | + | tig0022800:28158-28177 | Msa1402000:CDS | 50.0% | |
| GCCTGACAGATTACATCCCT+AGG | - | tig0022800:28162-28181 | None:intergenic | 50.0% | |
| ! | TCTTGATGACACCATAGCCG+TGG | - | tig0022800:28224-28243 | None:intergenic | 50.0% |
| !! | ACGGCTATGGTGTCATCAAG+AGG | + | tig0022800:28223-28242 | Msa1402000:CDS | 50.0% |
| TTGAATGGCAGGGCTTTCCT+TGG | + | tig0022800:28353-28372 | Msa1402000:intron | 50.0% | |
| AGTGGTTGCTCACACTTCCA+AGG | - | tig0022800:28373-28392 | None:intergenic | 50.0% | |
| ACAAGACACGCACAACCCAA+AGG | - | tig0022800:28553-28572 | None:intergenic | 50.0% | |
| GTTATGGGAAGCCACGGCTA+TGG | + | tig0022800:28210-28229 | Msa1402000:CDS | 55.0% | |
| GTTATGGGAAGCCACGGCTA+TGG | + | tig0022800:28210-28229 | Msa1402000:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0022800 | gene | 27517 | 28621 | 27517 | ID=Msa1402000;Name=Msa1402000 |
| tig0022800 | mRNA | 27517 | 28621 | 27517 | ID=Msa1402000-mRNA-1;Parent=Msa1402000;Name=Msa1402000-mRNA-1;_AED=0.08;_eAED=0.08;_QI=147|1|1|1|1|1|4|158|165 |
| tig0022800 | exon | 27517 | 27841 | 27517 | ID=Msa1402000-mRNA-1:exon:18686;Parent=Msa1402000-mRNA-1 |
| tig0022800 | exon | 27925 | 28031 | 27925 | ID=Msa1402000-mRNA-1:exon:18687;Parent=Msa1402000-mRNA-1 |
| tig0022800 | exon | 28132 | 28244 | 28132 | ID=Msa1402000-mRNA-1:exon:18688;Parent=Msa1402000-mRNA-1 |
| tig0022800 | exon | 28364 | 28621 | 28364 | ID=Msa1402000-mRNA-1:exon:18689;Parent=Msa1402000-mRNA-1 |
| tig0022800 | five_prime_UTR | 27517 | 27663 | 27517 | ID=Msa1402000-mRNA-1:five_prime_utr;Parent=Msa1402000-mRNA-1 |
| tig0022800 | CDS | 27664 | 27841 | 27664 | ID=Msa1402000-mRNA-1:cds;Parent=Msa1402000-mRNA-1 |
| tig0022800 | CDS | 27925 | 28031 | 27925 | ID=Msa1402000-mRNA-1:cds;Parent=Msa1402000-mRNA-1 |
| tig0022800 | CDS | 28132 | 28244 | 28132 | ID=Msa1402000-mRNA-1:cds;Parent=Msa1402000-mRNA-1 |
| tig0022800 | CDS | 28364 | 28463 | 28364 | ID=Msa1402000-mRNA-1:cds;Parent=Msa1402000-mRNA-1 |
| tig0022800 | three_prime_UTR | 28464 | 28621 | 28464 | ID=Msa1402000-mRNA-1:three_prime_utr;Parent=Msa1402000-mRNA-1 |
| Gene Sequence |
| Protein sequence |
