AlfalfaGEDB | Msa0424240

M. sativa cultivar zhongmu-4 / Msa0424240

KEGG: K18857
GO: -
PFAM: PF08240.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0424240	XP_003602129.1	98.864	352	4	0	1	352	1	352	0.0	721

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0424240	sp\|P12886\|ADH1_PEA	97.159	352	10	0	1	352	1	352	0.0	713

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0424240	A0A072V0U2	98.295	352	6	0	1	352	1	352	0.0	721

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0012800	Msa0424240	0.827948	1.179785e-54	-8.615850e-47
Msa0014960	Msa0424240	-0.801073	1.069168e-48	-8.615850e-47
Msa0031650	Msa0424240	0.835844	1.324257e-56	-8.615850e-47
Msa0036550	Msa0424240	0.867570	1.242034e-65	-8.615850e-47
Msa0036580	Msa0424240	0.867364	1.445139e-65	-8.615850e-47
Msa0038010	Msa0424240	0.807665	4.524691e-50	-8.615850e-47
Msa0039270	Msa0424240	0.825030	5.853682e-54	-8.615850e-47
Msa0039380	Msa0424240	0.808000	3.840391e-50	-8.615850e-47
Msa0043840	Msa0424240	0.832109	1.140315e-55	-8.615850e-47
Msa0046430	Msa0424240	0.807807	4.219762e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0424240	MtrunA17_Chr3g0125961	98.295	352	6	0	1	352	1	352	0.0	721
Msa0424240	MtrunA17_Chr3g0125911	98.864	352	4	0	1	352	1	352	0.0	721
Msa0424240	MtrunA17_Chr3g0125941	97.734	353	7	1	1	352	1	353	0.0	707
Msa0424240	MtrunA17_Chr7g0262421	59.829	351	141	0	2	352	3	353	2.18e-158	449
Msa0424240	MtrunA17_Chr1g0183131	58.166	349	146	0	4	352	3	351	7.81e-154	437
Msa0424240	MtrunA17_Chr6g0478081	50.278	360	174	3	2	360	3	358	7.95e-130	377
Msa0424240	MtrunA17_Chr7g0242291	49.020	357	176	5	1	352	12	367	2.16e-114	338
Msa0424240	MtrunA17_Chr5g0440521	44.258	357	196	3	7	360	16	372	2.18e-108	322
Msa0424240	MtrunA17_Chr3g0132351	48.066	362	181	5	5	360	12	372	5.92e-106	316
Msa0424240	MtrunA17_Chr3g0118441	42.179	358	204	3	6	360	10	367	3.40e-98	296

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0424240	AT1G77120.1	83.714	350	57	0	3	352	2	351	0.0	620
Msa0424240	AT5G43940.1	59.599	349	141	0	4	352	3	351	4.66e-157	446
Msa0424240	AT5G43940.2	57.341	361	142	1	4	352	3	363	8.99e-153	436
Msa0424240	AT5G24760.1	51.397	358	169	3	7	363	12	365	1.37e-135	392
Msa0424240	AT1G64710.1	50.559	358	171	3	3	354	2	359	3.75e-131	380
Msa0424240	AT1G32780.1	50.136	369	173	3	1	358	1	369	1.08e-130	380
Msa0424240	AT5G24760.2	50.000	340	165	3	25	363	1	336	2.25e-124	362
Msa0424240	AT5G24760.3	50.000	340	165	3	25	363	1	336	2.25e-124	362
Msa0424240	AT1G64710.2	49.405	336	164	3	25	354	1	336	1.57e-119	350
Msa0424240	AT5G42250.1	46.398	347	183	3	9	352	17	363	1.09e-107	321

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Find 131 sgRNAs with CRISPR-Local

Find 502 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGATAGCAACAGATGAAC+CGG	0.416086	3_2:-74447015	None:intergenic
AAAGTTTGTATTCTCAGCTG+TGG	0.560671	3_2:+74446575	Msa0424240:CDS
AACATCAGTATGACAAAGGG+AGG	0.627239	3_2:-74445556	None:intergenic
AACATTAGGAAGATCAGTGC+GGG	0.517924	3_2:-74447799	None:intergenic
AAGAACATAGTCCCCTAGTA+GGG	0.588537	3_2:-74448096	None:intergenic
AAGATAGCAACAGATGAACC+GGG	0.667433	3_2:-74447014	None:intergenic
AATAAACTCCCACAACGACA+AGG	0.658527	3_2:+74444239	None:intergenic
AATGTTGCTAAGCCGAAACC+CGG	0.606869	3_2:+74446996	Msa0424240:CDS
ACAAATTCAATAATCAGAAA+TGG	0.420697	3_2:-74448166	None:intergenic
ACAGGCAGGGCATGATCCCC+TGG	0.483648	3_2:-74446323	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAAAACAATAAATGTTGCC+TGG	+	chr3_2:74444607-74444626	Msa0424240:intron	30.0%
ATGCAAAAGTGACACATATT+TGG	+	chr3_2:74444670-74444689	Msa0424240:intron	30.0%
CCCAAAATTTATAAAGGAAG+AGG	+	chr3_2:74444918-74444937	Msa0424240:intron	30.0%
AGAAAGAAACACAATCCAAA+TGG	-	chr3_2:74445378-74445397	None:intergenic	30.0%
AGCTGCAGATATAAAATGAT+CGG	-	chr3_2:74445442-74445461	None:intergenic	30.0%
GTAAACATCAGTATGACAAA+GGG	-	chr3_2:74445562-74445581	None:intergenic	30.0%
AGTAAACATCAGTATGACAA+AGG	-	chr3_2:74445563-74445582	None:intergenic	30.0%
TCATACTGATGTTTACTTCT+GGG	+	chr3_2:74445566-74445585	Msa0424240:CDS	30.0%
TTATTCTTTCCTTCTGTTTC+AGG	+	chr3_2:74445832-74445851	Msa0424240:intron	30.0%
TATTCTTTCCTTCTGTTTCA+GGG	+	chr3_2:74445833-74445852	Msa0424240:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	74444242	74448353	74444242	ID=Msa0424240;Name=Msa0424240
chr3_2	mRNA	74444242	74448353	74444242	ID=Msa0424240-mRNA-1;Parent=Msa0424240;Name=Msa0424240-mRNA-1;_AED=0.09;_eAED=0.09;_QI=212\|1\|1\|1\|0.9\|0.90\|11\|328\|365
chr3_2	exon	74444242	74444392	74444242	ID=Msa0424240-mRNA-1:exon:18730;Parent=Msa0424240-mRNA-1
chr3_2	exon	74445202	74445299	74445202	ID=Msa0424240-mRNA-1:exon:18731;Parent=Msa0424240-mRNA-1
chr3_2	exon	74445460	74445596	74445460	ID=Msa0424240-mRNA-1:exon:18732;Parent=Msa0424240-mRNA-1
chr3_2	exon	74445854	74445900	74445854	ID=Msa0424240-mRNA-1:exon:18733;Parent=Msa0424240-mRNA-1
chr3_2	exon	74446283	74446608	74446283	ID=Msa0424240-mRNA-1:exon:18734;Parent=Msa0424240-mRNA-1
chr3_2	exon	74446979	74447061	74446979	ID=Msa0424240-mRNA-1:exon:18735;Parent=Msa0424240-mRNA-1
chr3_2	exon	74447150	74447225	74447150	ID=Msa0424240-mRNA-1:exon:18736;Parent=Msa0424240-mRNA-1
chr3_2	exon	74447316	74447377	74447316	ID=Msa0424240-mRNA-1:exon:18737;Parent=Msa0424240-mRNA-1
chr3_2	exon	74447496	74447591	74447496	ID=Msa0424240-mRNA-1:exon:18738;Parent=Msa0424240-mRNA-1
chr3_2	exon	74447681	74447842	74447681	ID=Msa0424240-mRNA-1:exon:18739;Parent=Msa0424240-mRNA-1
chr3_2	exon	74447954	74448353	74447954	ID=Msa0424240-mRNA-1:exon:18740;Parent=Msa0424240-mRNA-1
chr3_2	five_prime_UTR	74444242	74444392	74444242	ID=Msa0424240-mRNA-1:five_prime_utr;Parent=Msa0424240-mRNA-1
chr3_2	five_prime_UTR	74445202	74445262	74445202	ID=Msa0424240-mRNA-1:five_prime_utr;Parent=Msa0424240-mRNA-1
chr3_2	CDS	74445263	74445299	74445263	ID=Msa0424240-mRNA-1:cds;Parent=Msa0424240-mRNA-1
chr3_2	CDS	74445460	74445596	74445460	ID=Msa0424240-mRNA-1:cds;Parent=Msa0424240-mRNA-1
chr3_2	CDS	74445854	74445900	74445854	ID=Msa0424240-mRNA-1:cds;Parent=Msa0424240-mRNA-1
chr3_2	CDS	74446283	74446608	74446283	ID=Msa0424240-mRNA-1:cds;Parent=Msa0424240-mRNA-1
chr3_2	CDS	74446979	74447061	74446979	ID=Msa0424240-mRNA-1:cds;Parent=Msa0424240-mRNA-1
chr3_2	CDS	74447150	74447225	74447150	ID=Msa0424240-mRNA-1:cds;Parent=Msa0424240-mRNA-1
chr3_2	CDS	74447316	74447377	74447316	ID=Msa0424240-mRNA-1:cds;Parent=Msa0424240-mRNA-1
chr3_2	CDS	74447496	74447591	74447496	ID=Msa0424240-mRNA-1:cds;Parent=Msa0424240-mRNA-1
chr3_2	CDS	74447681	74447842	74447681	ID=Msa0424240-mRNA-1:cds;Parent=Msa0424240-mRNA-1
chr3_2	CDS	74447954	74448025	74447954	ID=Msa0424240-mRNA-1:cds;Parent=Msa0424240-mRNA-1
chr3_2	three_prime_UTR	74448026	74448353	74448026	ID=Msa0424240-mRNA-1:three_prime_utr;Parent=Msa0424240-mRNA-1

Gene Sequence

>Msa0424240

ATGTCGAACACTGTTGGTCAGGTCATCAAGTGCAGAGCTGCGGTTGCATGGGAGGCGGGGAAGCCGCTGGTGATGGAGGAAGTGGAGGTGGCGCCACCACAAGCCGGTGAAGTCCGTCTCAAGATACTCTTCACCTCCCTTTGTCATACTGATGTTTACTTCTGGGAAGCTAAGGGTCAAACTCCATTGTTTCCTCGTATTTTTGGTCATGAAGCTGGAGGGATTGTGGAGAGCGTAGGCGAGGGTGTGACACATCTAAAACCAGGGGATCATGCCCTGCCTGTATTCACAGGGGAGTGTGGGGACTGTCCACATTGTAAGTCAGAGGAGAGCAACATGTGTGATCTTCTTAGGATCAACACTGACAGGGGTGTCATGATCAATGACAATCAGTCAAGATTCTCTATTAAGGGACAGCCCATTCATCATTTCGTCGGCACATCGACATTCAGTGAATATACTGTTGTTCATGCTGGATGTGTTGCAAAGATCAACCCTGATGCACCACTTGACAAAGTTTGTATTCTCAGCTGTGGAATATGCACAGGTCTTGGTGCTACCATCAATGTTGCTAAGCCGAAACCCGGTTCATCTGTTGCTATCTTTGGACTTGGAGCTGTTGGCCTTGCTGCTGCTGAAGGAGCAAGGATATCTGGTGCATCAAGAATCATTGGAGTCGATTTAGTTTCTAGCCGATTTGAATTAGCTAAGAAGTTTGGGGTAAATGAGTTTGTGAACCCAAAAGATCATGACAAACCCGTACAACAGGTAATTGCTGAAATGACAAATGGAGGTGTGGATCGTGCTGTTGAATGTACTGGGAGCATCCAGGCCATGATCTCAGCTTTCGAATGTGTCCACGATGGTTGGGGTGTTGCTGTACTTGTTGGAGTTCCAAACAAAGATGATGCATTCAAAACTCACCCTATGAATTTGTTGAATGAAAGGACTCTTAAGGGTACCTTTTATGGTAACTACAAACCCCGCACTGATCTTCCTAATGTTGTAGAGAAGTACATGAAAGGGGAGCTGGAACTTGAGAAATTCATCACTCAATTCCATTTTCAGAGATTAACAAAGCTTTTGATTACATGCTGA

Protein sequence

>Msa0424240

MSNTVGQVIKCRAAVAWEAGKPLVMEEVEVAPPQAGEVRLKILFTSLCHTDVYFWEAKGQTPLFPRIFGHEAGGIVESVGEGVTHLKPGDHALPVFTGECGDCPHCKSEESNMCDLLRINTDRGVMINDNQSRFSIKGQPIHHFVGTSTFSEYTVVHAGCVAKINPDAPLDKVCILSCGICTGLGATINVAKPKPGSSVAIFGLGAVGLAAAEGARISGASRIIGVDLVSSRFELAKKFGVNEFVNPKDHDKPVQQVIAEMTNGGVDRAVECTGSIQAMISAFECVHDGWGVAVLVGVPNKDDAFKTHPMNLLNERTLKGTFYGNYKPRTDLPNVVEKYMKGELELEKFITQFHFQRLTKLLITC*