AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0648410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0648410 MtrunA17_Chr4g0039791 94.444 72 4 0 50 121 490 561 8.53e-41 142
Msa0648410 MtrunA17_Chr5g0409771 72.941 85 23 0 37 121 471 555 5.23e-34 123
Msa0648410 MtrunA17_Chr5g0409791 86.154 65 9 0 57 121 504 568 1.13e-31 117
Msa0648410 MtrunA17_Chr1g0157401 57.971 69 29 0 53 121 492 560 2.80e-21 87.8
Msa0648410 MtrunA17_Chr1g0197391 57.143 77 29 1 49 121 494 570 3.20e-20 84.7
Msa0648410 MtrunA17_Chr3g0116251 59.016 61 25 0 53 113 488 548 9.65e-20 83.2
Msa0648410 MtrunA17_Chr3g0116271 59.016 61 25 0 53 113 490 550 1.11e-19 83.2
Msa0648410 MtrunA17_Chr1g0180551 52.308 65 31 0 49 113 549 613 1.04e-17 77.4
Msa0648410 MtrunA17_Chr1g0186071 52.459 61 29 0 53 113 488 548 8.49e-17 75.1
Msa0648410 MtrunA17_Chr4g0072561 41.176 85 50 0 37 121 464 548 2.37e-16 73.6
Msa0648410 MtrunA17_Chr4g0000461 50.000 68 33 1 53 119 473 540 2.75e-15 70.5
Msa0648410 MtrunA17_Chr1g0180541 50.769 65 32 0 49 113 476 540 3.25e-15 70.5
Msa0648410 MtrunA17_Chr2g0334131 45.833 72 38 1 49 119 211 282 7.05e-15 68.6
Msa0648410 MtrunA17_Chr5g0395431 41.379 87 51 0 33 119 499 585 2.77e-14 67.8
Msa0648410 MtrunA17_Chr2g0327341 42.029 69 40 0 53 121 471 539 3.39e-14 67.4
Msa0648410 MtrunA17_Chr4g0028411 44.262 61 34 0 53 113 474 534 3.43e-14 67.4
Msa0648410 MtrunA17_Chr3g0102411 46.377 69 37 0 53 121 473 541 2.13e-13 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0648410 AT4G19010.1 51.020 98 42 2 25 121 470 562 5.02e-22 90.1
Msa0648410 AT1G20510.1 57.377 61 26 0 53 113 476 536 2.56e-19 82.4
Msa0648410 AT5G63380.1 57.377 61 26 0 53 113 490 550 2.96e-19 82.4
Msa0648410 AT1G51680.1 46.067 89 44 1 33 121 477 561 7.29e-18 78.6
Msa0648410 AT1G20480.1 50.725 69 34 0 53 121 497 565 7.66e-18 78.2
Msa0648410 AT3G21240.1 42.697 89 47 1 33 121 470 554 3.31e-17 76.6
Msa0648410 AT1G20500.1 52.174 69 33 0 53 121 482 550 3.54e-17 76.3
Msa0648410 AT5G38120.1 54.098 61 28 0 53 113 482 542 7.40e-17 75.5
Msa0648410 AT1G65060.1 54.839 62 28 0 53 114 496 557 9.62e-16 72.4
Msa0648410 AT4G05160.1 39.326 89 54 0 33 121 456 544 2.55e-15 71.2
Msa0648410 AT3G21230.1 45.070 71 39 0 49 119 496 566 3.28e-13 65.1
Msa0648410 AT1G62940.1 50.000 62 31 0 53 114 472 533 8.41e-12 61.2

Find 204 sgRNAs with CRISPR-Local

Find 317 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTTCTGCTTTACACAAAA+TGG 0.138296 4_3:+54884399 Msa0648410:five_prime_UTR
GCTGGTATATTGTATTCTTC+TGG 0.174054 4_3:+54884763 Msa0648410:five_prime_UTR
ACAGGATAGTAAATTGAATT+TGG 0.180607 4_3:-54884457 None:intergenic
CTCTTGGCTGTTCTGTATTT+AGG 0.185255 4_3:+54884484 Msa0648410:five_prime_UTR
ATATGACCAATAATGAATTT+TGG 0.185466 4_3:+54883703 Msa0648410:five_prime_UTR
ACTTGTCGGTTGTGCCTTAA+AGG 0.192922 4_3:-54883454 None:intergenic
GCCCTTATTGATTCTTCATC+TGG 0.217618 4_3:+54884331 Msa0648410:five_prime_UTR
TAATCATAATGTTGGGGATT+TGG 0.219662 4_3:-54883923 None:intergenic
GGAGTTTGATTAAAAGAGTT+TGG 0.228239 4_3:-54883618 None:intergenic
TGTTGGGGATTTGGAATATT+TGG 0.231225 4_3:-54883914 None:intergenic
TATCAAATACAAGGGATTTC+AGG 0.243292 4_3:+54886648 Msa0648410:five_prime_UTR
GTTGAGTGATCATGTTGTTT+TGG 0.245364 4_3:-54884177 None:intergenic
CGCCATGTATTGTCTTTCTT+TGG 0.245550 4_3:-54887350 None:intergenic
TTTCATGAATTATAGGATAT+TGG 0.247502 4_3:+54888078 Msa0648410:three_prime_UTR
TAATATCCAAAATTCATTAT+TGG 0.251066 4_3:-54883709 None:intergenic
TTGACATTGACGAGGTTATC+AGG 0.253484 4_3:+54884998 Msa0648410:five_prime_UTR
GATTAAGTGCAATATATTAT+AGG 0.261460 4_3:+54883544 Msa0648410:five_prime_UTR
CTTCATCTTCGGCACTGATA+TGG 0.264781 4_3:-54886973 None:intergenic
AGACAAAAGAATACAATTTC+AGG 0.266973 4_3:+54888279 Msa0648410:three_prime_UTR
ACGGCTGCATCGACTATTTC+AGG 0.272026 4_3:-54886820 None:intergenic
AAATTTCCAACCCTATTCTT+AGG 0.276628 4_3:+54883855 Msa0648410:five_prime_UTR
TAAAATATAACTCTTTCTTT+AGG 0.278247 4_3:+54883641 Msa0648410:five_prime_UTR
AGTGCCGAAGATGAAGAAAT+TGG 0.290280 4_3:+54886980 Msa0648410:CDS
TCATAGAAATCTAATTTCTA+TGG 0.293867 4_3:+54884819 Msa0648410:five_prime_UTR
TTTCACAACTATTCGTCAAT+AGG 0.295826 4_3:+54885833 Msa0648410:five_prime_UTR
GGGTCAGTTGGAAGGTTTCT+AGG 0.297530 4_3:-54884256 None:intergenic
ATGAAGGCAAAGGGTGTTAA+TGG 0.303488 4_3:+54885084 Msa0648410:five_prime_UTR
AATCTTCTAAAGTGCCTCTT+TGG 0.304882 4_3:+54886914 Msa0648410:CDS
TGGAAATTTCTGTACGCATT+TGG 0.315063 4_3:-54883841 None:intergenic
AATATTTGGTGTGAAGAAAA+AGG 0.315910 4_3:-54883900 None:intergenic
TTAATACGTTAGTCTTCTTC+AGG 0.327390 4_3:+54887805 Msa0648410:three_prime_UTR
CAGGCAGCTGCGGTGAGCTT+TGG 0.328932 4_3:+54885918 Msa0648410:five_prime_UTR
AATGTTAATATGTACCTTTA+AGG 0.329026 4_3:+54883440 Msa0648410:five_prime_UTR
CAAAGCTCACCGCAGCTGCC+TGG 0.332403 4_3:-54885917 None:intergenic
TTGCCAATGTTTCATGTGTA+TGG 0.342668 4_3:+54884913 Msa0648410:five_prime_UTR
AGTCCACCAGTTTCATGTTA+TGG 0.343233 4_3:-54887378 None:intergenic
GGAACGTAATAGCTACGTTT+AGG 0.343445 4_3:-54883951 None:intergenic
AATGTGCAGAATAGAAATTT+TGG 0.344789 4_3:-54887477 None:intergenic
CATTTAACTCATTAACTTGT+CGG 0.346300 4_3:-54883468 None:intergenic
CTGGTATATTGTATTCTTCT+GGG 0.346881 4_3:+54884764 Msa0648410:five_prime_UTR
ACCCAGATGAAGAATCAATA+AGG 0.350199 4_3:-54884333 None:intergenic
GGTTATAAAGATTGAAGCTT+TGG 0.356356 4_3:-54884070 None:intergenic
AGCAGTGCCTGAGTTGCTTT+CGG 0.356544 4_3:-54887277 None:intergenic
AGTCATTGGTTTACAATACT+TGG 0.360941 4_3:+54887969 Msa0648410:three_prime_UTR
AAGGGTGAAATTGTCTTTGT+TGG 0.360994 4_3:-54883881 None:intergenic
GGGACTATAATCATAATGTT+GGG 0.361470 4_3:-54883930 None:intergenic
ACATCTGAATTATGAGTCAT+TGG 0.365621 4_3:+54887955 Msa0648410:three_prime_UTR
TTCCCCAATTTCTTCATCTT+CGG 0.365786 4_3:-54886984 None:intergenic
TTCTTACCTCTCATAATAGA+AGG 0.374837 4_3:-54885950 None:intergenic
TTCACAACTATTCGTCAATA+GGG 0.376437 4_3:+54885834 Msa0648410:five_prime_UTR
CACTAATAACAGAATCATTC+AGG 0.378618 4_3:+54887866 Msa0648410:three_prime_UTR
ACCTGTATAAAATCAACGTT+AGG 0.379226 4_3:-54885192 None:intergenic
TGTTGGTGAAATTCGTAAAC+AGG 0.387052 4_3:+54884540 Msa0648410:five_prime_UTR
TTCACTTGTAATCTTTGATA+AGG 0.390882 4_3:+54887769 Msa0648410:three_prime_UTR
AAAGCTCACCGCAGCTGCCT+GGG 0.391998 4_3:-54885916 None:intergenic
TTACTATCCTGTTATACTCT+TGG 0.392695 4_3:+54884468 Msa0648410:five_prime_UTR
AGAACAGCCAAGAGTATAAC+AGG 0.393213 4_3:-54884475 None:intergenic
GAGTTTGATTAAAAGAGTTT+GGG 0.395162 4_3:-54883617 None:intergenic
GAACGTAATAGCTACGTTTA+GGG 0.395890 4_3:-54883950 None:intergenic
ACCATAAGTCTCATGTAGAT+TGG 0.396872 4_3:+54888202 Msa0648410:three_prime_UTR
GTGCCGAAGATGAAGAAATT+GGG 0.397388 4_3:+54886981 Msa0648410:CDS
AGGGTGAAATTGTCTTTGTT+GGG 0.398587 4_3:-54883880 None:intergenic
CCCAATCTACATGAGACTTA+TGG 0.402140 4_3:-54888203 None:intergenic
ACCTTCCGGACCTTCTTGTA+TGG 0.403187 4_3:-54887209 None:intergenic
TGCTGCGGGATTATTGTCGT+TGG 0.410245 4_3:+54884948 Msa0648410:five_prime_UTR
TGTTGTTCCACCGATGTTGT+CGG 0.411176 4_3:+54885053 Msa0648410:five_prime_UTR
AAGGAAACAATGTCAAGAAA+AGG 0.413052 4_3:-54884277 None:intergenic
CCCTTATTGATTCTTCATCT+GGG 0.414327 4_3:+54884332 Msa0648410:five_prime_UTR
TGCTTGAGTTCTAAACTCTA+AGG 0.416696 4_3:+54887296 Msa0648410:CDS
TCAGGATGGATATTTACATT+TGG 0.418131 4_3:+54886606 Msa0648410:five_prime_UTR
TTTGTTGGGATCCTAAGAAT+AGG 0.418373 4_3:-54883866 None:intergenic
GTCAAGAAACTAGAGCTATT+AGG 0.418468 4_3:+54884607 Msa0648410:five_prime_UTR
TCAAAGGGTAGTTATTAAAC+AGG 0.419883 4_3:+54884732 Msa0648410:five_prime_UTR
ATTAAACAAAGCCAAGGAAA+AGG 0.423333 4_3:-54883760 None:intergenic
CTATTCGTCAATAGGGTTGT+TGG 0.424322 4_3:+54885841 Msa0648410:five_prime_UTR
AGAGGCACTTTAGAAGATTA+CGG 0.425783 4_3:-54886910 None:intergenic
GACAACATCGGTGGAACAAC+AGG 0.427365 4_3:-54885051 None:intergenic
TTGTTGGGATCCTAAGAATA+GGG 0.428757 4_3:-54883865 None:intergenic
TACGCATTTGGCTCTCAAGT+TGG 0.428976 4_3:-54883829 None:intergenic
TGTTTAATAACTACCCTTTG+AGG 0.430394 4_3:-54884730 None:intergenic
GGTTTATCGCTCTTTGCTGC+GGG 0.430597 4_3:+54884934 Msa0648410:five_prime_UTR
ACGCATTTGGCTCTCAAGTT+GGG 0.434343 4_3:-54883828 None:intergenic
TAACAACTTTGAGTAAGAAA+TGG 0.436153 4_3:-54884359 None:intergenic
TTTGTTGAAAGTGATGGATT+CGG 0.436910 4_3:-54888038 None:intergenic
AGGGACTATAATCATAATGT+TGG 0.443278 4_3:-54883931 None:intergenic
CAGAAAATGAGAAGGATTTG+AGG 0.445513 4_3:+54884656 Msa0648410:five_prime_UTR
CTCAACACCCTCACCCTCAT+TGG 0.448612 4_3:+54884194 Msa0648410:five_prime_UTR
TGCGGTGAGCTTTGGTTGAG+AGG 0.454034 4_3:+54885926 Msa0648410:five_prime_UTR
GCTTCTGCTTTACACAAAAT+GGG 0.455667 4_3:+54884400 Msa0648410:five_prime_UTR
GGGTTACCATAACATGAAAC+TGG 0.457748 4_3:+54887372 Msa0648410:three_prime_UTR
GCTGCGGGATTATTGTCGTT+GGG 0.458574 4_3:+54884949 Msa0648410:five_prime_UTR
TTGTATTCTTCTGGGACTAC+AGG 0.459563 4_3:+54884772 Msa0648410:five_prime_UTR
CCATAAGTCTCATGTAGATT+GGG 0.460455 4_3:+54888203 Msa0648410:three_prime_UTR
CCCAGATGAAGAATCAATAA+GGG 0.467167 4_3:-54884332 None:intergenic
ATAACTGACTAAAGGTGTAC+AGG 0.470744 4_3:-54887578 None:intergenic
ATTGAAAAGTATGACACAAC+AGG 0.473687 4_3:-54887712 None:intergenic
TAAATTTGATTTGCCTCAAA+GGG 0.485047 4_3:+54884717 Msa0648410:five_prime_UTR
GGTGCAGCGCCTTTGAGCAT+AGG 0.486009 4_3:+54885141 Msa0648410:five_prime_UTR
GTAAATTTGATTTGCCTCAA+AGG 0.487083 4_3:+54884716 Msa0648410:five_prime_UTR
AACAGCTAGAAACACACTCT+TGG 0.487245 4_3:-54884890 None:intergenic
GCATTTGTTGTCAAGAAGGT+TGG 0.487321 4_3:+54887016 Msa0648410:CDS
ACATAATCGACCACATGCTT+CGG 0.487322 4_3:-54887052 None:intergenic
ATTTCACCAACACTACTAAG+AGG 0.489624 4_3:-54884529 None:intergenic
CGGAGACCTTGGTATCTTGT+CGG 0.489928 4_3:-54887240 None:intergenic
AGTGGCATTTGTTGTCAAGA+AGG 0.496940 4_3:+54887012 Msa0648410:CDS
GTACAGGGCAGAGTAATTGA+TGG 0.498066 4_3:-54887562 None:intergenic
TTGAGCATAGGAGTTATTAG+TGG 0.499006 4_3:+54885153 Msa0648410:five_prime_UTR
AACACAGCTTCTAGATCAGC+AGG 0.500308 4_3:-54886787 None:intergenic
CACAAAATGGGTGTTTCACA+AGG 0.502968 4_3:+54884412 Msa0648410:five_prime_UTR
TTGAATCCTCTTAGTAGTGT+TGG 0.505325 4_3:+54884523 Msa0648410:five_prime_UTR
ATCTTCCCAGTCGGAGACCT+TGG 0.507080 4_3:-54887251 None:intergenic
TGGTTTATCGCTCTTTGCTG+CGG 0.507976 4_3:+54884933 Msa0648410:five_prime_UTR
ATTTCTCTAGTGCATTTACA+AGG 0.509552 4_3:-54887611 None:intergenic
TGTCGGTGAAGAACACCTTC+CGG 0.510625 4_3:-54887223 None:intergenic
TTCTTGACAACAAATGCCAC+TGG 0.510888 4_3:-54887010 None:intergenic
TAAGTTGCAGAGTTTGAGAC+AGG 0.511530 4_3:+54885110 Msa0648410:five_prime_UTR
TTGCTCCATACAAGAAGGTC+CGG 0.515213 4_3:+54887204 Msa0648410:CDS
CGGAGGCAAAAGTGGTAGAC+TGG 0.515561 4_3:+54885873 Msa0648410:five_prime_UTR
AAAGATGGTTGGCTACATAC+AGG 0.515947 4_3:+54886565 Msa0648410:five_prime_UTR
GCGGTGAGCTTTGGTTGAGA+GGG 0.517348 4_3:+54885927 Msa0648410:five_prime_UTR
TTAAACAAAGCCAAGGAAAA+GGG 0.518391 4_3:-54883759 None:intergenic
AGGAAACAATGTCAAGAAAA+GGG 0.521407 4_3:-54884276 None:intergenic
TTTGTCTTTATCATGTGTGC+GGG 0.521901 4_3:+54883785 Msa0648410:five_prime_UTR
AAGCTCACCGCAGCTGCCTG+GGG 0.523366 4_3:-54885915 None:intergenic
TGGGATCCTAAGAATAGGGT+TGG 0.527873 4_3:-54883861 None:intergenic
ATGTCAAGAAAAGGGTCAGT+TGG 0.533509 4_3:-54884268 None:intergenic
AAACCATACACATGAAACAT+TGG 0.533547 4_3:-54884916 None:intergenic
TCCAAACACGGAGGCAAAAG+TGG 0.534472 4_3:+54885865 Msa0648410:five_prime_UTR
GAAGCCATGGATTTGACTAA+AGG 0.537715 4_3:-54884382 None:intergenic
ATATCAACAATTGATAAAGA+TGG 0.537763 4_3:+54886550 Msa0648410:five_prime_UTR
ATCATCAATAACTGATCAAG+TGG 0.539714 4_3:+54883307 Msa0648410:five_prime_UTR
GTTACCTTTAGTCAAATCCA+TGG 0.539728 4_3:+54884378 Msa0648410:five_prime_UTR
AGATCCTCCGAAAGCAACTC+AGG 0.540928 4_3:+54887270 Msa0648410:CDS
AAAGTGGTAGACTGGAACAA+TGG 0.541163 4_3:+54885881 Msa0648410:five_prime_UTR
CTCAGGATACTTGAATAATG+AGG 0.541417 4_3:+54886519 Msa0648410:intron
CACATCTCATTATGTTTGAG+AGG 0.543063 4_3:+54888121 Msa0648410:three_prime_UTR
TTCATATACTTACGCTGTAA+CGG 0.543086 4_3:-54886839 None:intergenic
CTACATGAGACTTATGGTAA+TGG 0.544122 4_3:-54888197 None:intergenic
CAAGAAAAGGGTCAGTTGGA+AGG 0.544290 4_3:-54884264 None:intergenic
AACGAATTTCAAGAAAGTCA+TGG 0.548135 4_3:+54883374 Msa0648410:five_prime_UTR
CTCTTACTATAACTGACTAA+AGG 0.548149 4_3:-54887586 None:intergenic
AATCAACGTTAGGGAAAGCA+TGG 0.550452 4_3:-54885182 None:intergenic
GAAAGACATTATCAAATACA+AGG 0.550958 4_3:+54886639 Msa0648410:five_prime_UTR
GGTCGATTATGTTGCTGAGC+AGG 0.550976 4_3:+54887063 Msa0648410:CDS
GTGGAAATGGGGATATGAAA+GGG 0.551077 4_3:-54883972 None:intergenic
GGGACTACAGGTGTGAGTAA+AGG 0.553349 4_3:+54884784 Msa0648410:five_prime_UTR
TTATAGGATATTGGATCAGA+GGG 0.554300 4_3:+54888087 Msa0648410:three_prime_UTR
TTTGTGTAAAGCAGAAGCCA+TGG 0.560434 4_3:-54884395 None:intergenic
CCTGTATAAAATCAACGTTA+GGG 0.562718 4_3:-54885191 None:intergenic
CTTGAAACAATCAATTCTGA+GGG 0.563240 4_3:+54887927 Msa0648410:three_prime_UTR
TGACATTGACGAGGTTATCA+GGG 0.564262 4_3:+54884999 Msa0648410:five_prime_UTR
ACACTACTAAGAGGATTCAA+TGG 0.565007 4_3:-54884520 None:intergenic
TACCAAAGAAAGACAATACA+TGG 0.567298 4_3:+54887348 Msa0648410:three_prime_UTR
ACTTGAAACAATCAATTCTG+AGG 0.568297 4_3:+54887926 Msa0648410:three_prime_UTR
CAACAATTGATAAAGATGGT+TGG 0.569594 4_3:+54886554 Msa0648410:five_prime_UTR
GTGGTGTGAAGTGTTGAATG+TGG 0.569778 4_3:-54884091 None:intergenic
GGCATTGACAATGAAGGCAA+AGG 0.569984 4_3:+54885074 Msa0648410:five_prime_UTR
GCATTGACAATGAAGGCAAA+GGG 0.570042 4_3:+54885075 Msa0648410:five_prime_UTR
ATTTGATAATGTCTTTCAAG+CGG 0.579224 4_3:-54886633 None:intergenic
ATTATAGGATATTGGATCAG+AGG 0.579489 4_3:+54888086 Msa0648410:three_prime_UTR
ATTGTCAATGCCGACAACAT+CGG 0.579863 4_3:-54885063 None:intergenic
GGACTATAATCATAATGTTG+GGG 0.580599 4_3:-54883929 None:intergenic
TGTGGAAATGGGGATATGAA+AGG 0.582479 4_3:-54883973 None:intergenic
GTTCAAATTAGCAAAGGTGC+AGG 0.584639 4_3:+54888015 Msa0648410:three_prime_UTR
AAAGAAAGACAATACATGGC+GGG 0.585051 4_3:+54887352 Msa0648410:three_prime_UTR
TGCTGTGCCAGAAAATGAGA+AGG 0.586565 4_3:+54884648 Msa0648410:five_prime_UTR
AGGCTGTGGCTTGAGTGTGA+AGG 0.586939 4_3:-54884005 None:intergenic
AGTTTGAGACAGGTCTCTTG+CGG 0.592614 4_3:+54885120 Msa0648410:five_prime_UTR
GATGAATACCAATGAGGGTG+AGG 0.592922 4_3:-54884202 None:intergenic
GTTGTCGGCATTGACAATGA+AGG 0.595731 4_3:+54885068 Msa0648410:five_prime_UTR
AATACTTGGAAAATTTCTGT+CGG 0.596557 4_3:+54887983 Msa0648410:three_prime_UTR
GATCTACTGTCATTGTAATG+AGG 0.600626 4_3:+54884971 Msa0648410:five_prime_UTR
TGCCGAAGATGAAGAAATTG+GGG 0.602629 4_3:+54886982 Msa0648410:CDS
AAGATACCAAGGTCTCCGAC+TGG 0.604082 4_3:+54887245 Msa0648410:CDS
CTAATAACTCCTATGCTCAA+AGG 0.604731 4_3:-54885150 None:intergenic
ATTAAACAGGAAGATACTGC+TGG 0.608838 4_3:+54884745 Msa0648410:five_prime_UTR
CGTAAACAGGTTCAAGAGTG+TGG 0.611754 4_3:+54884553 Msa0648410:five_prime_UTR
AGGGTTGTTGGCTCCAAACA+CGG 0.614077 4_3:+54885853 Msa0648410:five_prime_UTR
AAAGACATTATCAAATACAA+GGG 0.615550 4_3:+54886640 Msa0648410:five_prime_UTR
GTCAATGCCGACAACATCGG+TGG 0.615987 4_3:-54885060 None:intergenic
TTTCGGAGGATCTTCCCAGT+CGG 0.617027 4_3:-54887260 None:intergenic
AGAGGGCCTTCTATTATGAG+AGG 0.618979 4_3:+54885944 Msa0648410:five_prime_UTR
AGATACCAAGGTCTCCGACT+GGG 0.620788 4_3:+54887246 Msa0648410:CDS
GCAGGTTGCTCCATACAAGA+AGG 0.622353 4_3:+54887199 Msa0648410:intron
TCGACTGCAGTGGGAACACG+TGG 0.624008 4_3:+54885794 Msa0648410:five_prime_UTR
AACTGCGTTCAAATTAGCAA+AGG 0.630302 4_3:+54888009 Msa0648410:three_prime_UTR
TAACTGACTAAAGGTGTACA+GGG 0.630429 4_3:-54887577 None:intergenic
GCACTTTAGAAGATTACGGA+TGG 0.635858 4_3:-54886906 None:intergenic
TTACCATAACATGAAACTGG+TGG 0.637363 4_3:+54887375 Msa0648410:three_prime_UTR
AGCTCACCGCAGCTGCCTGG+GGG 0.641341 4_3:-54885914 None:intergenic
AGGGCTAAAACCCCATCATG+AGG 0.644178 4_3:-54884313 None:intergenic
AGTGCCTGAGTTGCTTTCGG+AGG 0.646420 4_3:-54887274 None:intergenic
ATGACTGAGTCGACTGCAGT+GGG 0.649133 4_3:+54885785 Msa0648410:five_prime_UTR
TTATAATCAAATGTCCAAAG+AGG 0.651413 4_3:-54886928 None:intergenic
TCCATACAAGAAGGTCCGGA+AGG 0.659172 4_3:+54887208 Msa0648410:CDS
TGTACTTTCTCCGAAGCATG+TGG 0.668913 4_3:+54887042 Msa0648410:CDS
AATGACTGAGTCGACTGCAG+TGG 0.680151 4_3:+54885784 Msa0648410:five_prime_UTR
AAGAAACTAGAGCTATTAGG+TGG 0.680245 4_3:+54884610 Msa0648410:five_prime_UTR
GAGGAAATTTGACATTGACG+AGG 0.683873 4_3:+54884990 Msa0648410:five_prime_UTR
ATGAATACCAATGAGGGTGA+GGG 0.691317 4_3:-54884201 None:intergenic
AGAAATTGGGGAAATTCCAG+TGG 0.696561 4_3:+54886994 Msa0648410:CDS
TCTTCACCGACAAGATACCA+AGG 0.749835 4_3:+54887234 Msa0648410:CDS
CAAAGAAAGACAATACATGG+CGG 0.750110 4_3:+54887351 Msa0648410:three_prime_UTR
GTTGTTGGCTCCAAACACGG+AGG 0.795141 4_3:+54885856 Msa0648410:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAGAAAAAAAATAAAATAA+AGG - chr4_3:54887177-54887196 None:intergenic 10.0%
!!! TTATTTTATTTTTTTTCTTC+TGG + chr4_3:54887177-54887196 Msa0648410:intron 10.0%
!! TAAAAATTAAATTCAATGCA+CGG - chr4_3:54883514-54883533 None:intergenic 15.0%
!!! TAAAGAAAGAGTTATATTTT+AGG - chr4_3:54883642-54883661 None:intergenic 15.0%
!! TAAAATATAACTCTTTCTTT+AGG + chr4_3:54883641-54883660 Msa0648410:five_prime_UTR 15.0%
!!! ATATTTTTGTGTGTAAAAAA+AGG - chr4_3:54883687-54883706 None:intergenic 15.0%
!! TAATATCCAAAATTCATTAT+TGG - chr4_3:54883712-54883731 None:intergenic 15.0%
!! AAATAGTGAAATAGAAAAAA+GGG - chr4_3:54884045-54884064 None:intergenic 15.0%
!! AAAATAGTGAAATAGAAAAA+AGG - chr4_3:54884046-54884065 None:intergenic 15.0%
!!! TTATTTTTCTTTGTTATTGT+TGG + chr4_3:54885297-54885316 Msa0648410:intron 15.0%
!!! TTATTAACATTTTACTGATA+AGG - chr4_3:54886280-54886299 None:intergenic 15.0%
!!! GTTTGATTAACAATTTTAAT+TGG - chr4_3:54886485-54886504 None:intergenic 15.0%
!!! GTAATATTTTCATGAATTAT+AGG + chr4_3:54888071-54888090 Msa0648410:three_prime_UTR 15.0%
!! AACAATAACTAAAAACTCTT+TGG + chr4_3:54883415-54883434 Msa0648410:five_prime_UTR 20.0%
!! ACAATAACTAAAAACTCTTT+GGG + chr4_3:54883416-54883435 Msa0648410:five_prime_UTR 20.0%
!! AATGTTAATATGTACCTTTA+AGG + chr4_3:54883440-54883459 Msa0648410:five_prime_UTR 20.0%
!! GATTAAGTGCAATATATTAT+AGG + chr4_3:54883544-54883563 Msa0648410:five_prime_UTR 20.0%
!!! ATATGACCAATAATGAATTT+TGG + chr4_3:54883703-54883722 Msa0648410:five_prime_UTR 20.0%
!!! AGTTTTTTTAACGTTCTTTA+TGG + chr4_3:54884694-54884713 Msa0648410:five_prime_UTR 20.0%
!! TCATAGAAATCTAATTTCTA+TGG + chr4_3:54884819-54884838 Msa0648410:five_prime_UTR 20.0%
!! TTTAATAGCAACTGTTATAT+AGG + chr4_3:54885378-54885397 Msa0648410:intron 20.0%
!! TTAATAGCAACTGTTATATA+GGG + chr4_3:54885379-54885398 Msa0648410:intron 20.0%
!!! CAAATAAAGTTCAACTTTTA+TGG - chr4_3:54885696-54885715 None:intergenic 20.0%
!! AGATATTAAAAAGTGAAACA+CGG + chr4_3:54885991-54886010 Msa0648410:intron 20.0%
!! TATAGAGCAAATATAGTAAA+GGG - chr4_3:54886107-54886126 None:intergenic 20.0%
!! ATATAGAGCAAATATAGTAA+AGG - chr4_3:54886108-54886127 None:intergenic 20.0%
!! ATATCAACAATTGATAAAGA+TGG + chr4_3:54886550-54886569 Msa0648410:five_prime_UTR 20.0%
!!! AGATATTGTTTATTTTGATC+AGG + chr4_3:54886588-54886607 Msa0648410:five_prime_UTR 20.0%
!! AAAGACATTATCAAATACAA+GGG + chr4_3:54886640-54886659 Msa0648410:five_prime_UTR 20.0%
!!! ATCATTTGTTTTGATTACTT+GGG - chr4_3:54887842-54887861 None:intergenic 20.0%
!!! TATCATTTGTTTTGATTACT+TGG - chr4_3:54887843-54887862 None:intergenic 20.0%
!! TTTCATGAATTATAGGATAT+TGG + chr4_3:54888078-54888097 Msa0648410:three_prime_UTR 20.0%
!! CTAAATACCTCTAAAAAAAG+TGG + chr4_3:54883335-54883354 Msa0648410:five_prime_UTR 25.0%
!!! CATTTAACTCATTAACTTGT+CGG - chr4_3:54883471-54883490 None:intergenic 25.0%
! GAGTTTGATTAAAAGAGTTT+GGG - chr4_3:54883620-54883639 None:intergenic 25.0%
! TTTGTGTGTAAAAAAAGGTT+TGG - chr4_3:54883682-54883701 None:intergenic 25.0%
! CAAAAAATTAAACAAAGCCA+AGG - chr4_3:54883769-54883788 None:intergenic 25.0%
!!! ATATTTGGTGTGAAGAAAAA+GGG - chr4_3:54883902-54883921 None:intergenic 25.0%
!!! AATATTTGGTGTGAAGAAAA+AGG - chr4_3:54883903-54883922 None:intergenic 25.0%
!! GATTTTCAGAAAAAAGAGAA+CGG - chr4_3:54884121-54884140 None:intergenic 25.0%
!!! CTCTTTTTTCTGAAAATCAA+TGG + chr4_3:54884123-54884142 Msa0648410:five_prime_UTR 25.0%
!! TTTTTGATGAATACCAATGA+GGG - chr4_3:54884210-54884229 None:intergenic 25.0%
! TAACAACTTTGAGTAAGAAA+TGG - chr4_3:54884362-54884381 None:intergenic 25.0%
! ACAGGATAGTAAATTGAATT+TGG - chr4_3:54884460-54884479 None:intergenic 25.0%
!!! TAAATTTGATTTGCCTCAAA+GGG + chr4_3:54884717-54884736 Msa0648410:five_prime_UTR 25.0%
! ATTAAGGCTATTCTATTCAA+AGG - chr4_3:54885224-54885243 None:intergenic 25.0%
! TTACTTGCATTCTCTAATTA+AGG - chr4_3:54885240-54885259 None:intergenic 25.0%
! AATAAATTGTTTAGCCAAAC+AGG - chr4_3:54885405-54885424 None:intergenic 25.0%
! AAATAAGCTGAAAAGAACTA+AGG + chr4_3:54885555-54885574 Msa0648410:intron 25.0%
!! TGTAAGATTGTTTCCTTTTA+GGG + chr4_3:54885755-54885774 Msa0648410:intron 25.0%
! ATATAAAGGTCTTGATTCAA+TGG + chr4_3:54886019-54886038 Msa0648410:intron 25.0%
! ATAGAGCAAATATAGTAAAG+GGG - chr4_3:54886106-54886125 None:intergenic 25.0%
!! ATTTGTTAACGCTTAACTAA+AGG + chr4_3:54886126-54886145 Msa0648410:intron 25.0%
! AACTAACATGATATTTCTCA+AGG - chr4_3:54886159-54886178 None:intergenic 25.0%
! AAACTGAAGTAAATTTCTCT+TGG + chr4_3:54886367-54886386 Msa0648410:intron 25.0%
!!! ATTGTTTATTTTGATCAGGA+TGG + chr4_3:54886592-54886611 Msa0648410:five_prime_UTR 25.0%
!!! ATTTGATAATGTCTTTCAAG+CGG - chr4_3:54886636-54886655 None:intergenic 25.0%
! GAAAGACATTATCAAATACA+AGG + chr4_3:54886639-54886658 Msa0648410:five_prime_UTR 25.0%
! TTATAATCAAATGTCCAAAG+AGG - chr4_3:54886931-54886950 None:intergenic 25.0%
!!! TTTATTTTTTTTCTTCTGGC+AGG + chr4_3:54887181-54887200 Msa0648410:intron 25.0%
!! TCATGAGTACATCTTTTTAA+AGG + chr4_3:54887422-54887441 Msa0648410:three_prime_UTR 25.0%
!! AATGTGCAGAATAGAAATTT+TGG - chr4_3:54887480-54887499 None:intergenic 25.0%
! AATACTTGGAAAATTTCTGT+CGG + chr4_3:54887983-54888002 Msa0648410:three_prime_UTR 25.0%
! ACTTTCAACAAAAAGACATT+TGG + chr4_3:54888049-54888068 Msa0648410:three_prime_UTR 25.0%
!!! TAACATGATTTTAGCTAAGA+AGG + chr4_3:54888161-54888180 Msa0648410:three_prime_UTR 25.0%
!! AATTGTATTCTTTTGTCTTC+TGG - chr4_3:54888277-54888296 None:intergenic 25.0%
ATCATCAATAACTGATCAAG+TGG + chr4_3:54883307-54883326 Msa0648410:five_prime_UTR 30.0%
!!! AGACTAACCACTTTTTTTAG+AGG - chr4_3:54883345-54883364 None:intergenic 30.0%
AACGAATTTCAAGAAAGTCA+TGG + chr4_3:54883374-54883393 Msa0648410:five_prime_UTR 30.0%
GGAGTTTGATTAAAAGAGTT+TGG - chr4_3:54883621-54883640 None:intergenic 30.0%
! ATATTTTATGCCCTTTTCCT+TGG + chr4_3:54883749-54883768 Msa0648410:five_prime_UTR 30.0%
TTAAACAAAGCCAAGGAAAA+GGG - chr4_3:54883762-54883781 None:intergenic 30.0%
ATTAAACAAAGCCAAGGAAA+AGG - chr4_3:54883763-54883782 None:intergenic 30.0%
! TTTTGTCTTTATCATGTGTG+CGG + chr4_3:54883784-54883803 Msa0648410:five_prime_UTR 30.0%
AAATTTCCAACCCTATTCTT+AGG + chr4_3:54883855-54883874 Msa0648410:five_prime_UTR 30.0%
TAATCATAATGTTGGGGATT+TGG - chr4_3:54883926-54883945 None:intergenic 30.0%
GGACTATAATCATAATGTTG+GGG - chr4_3:54883932-54883951 None:intergenic 30.0%
GGGACTATAATCATAATGTT+GGG - chr4_3:54883933-54883952 None:intergenic 30.0%
AGGGACTATAATCATAATGT+TGG - chr4_3:54883934-54883953 None:intergenic 30.0%
GGTTATAAAGATTGAAGCTT+TGG - chr4_3:54884073-54884092 None:intergenic 30.0%
!!! TTGAGTGATCATGTTGTTTT+GGG - chr4_3:54884179-54884198 None:intergenic 30.0%
! GTTTTTGATGAATACCAATG+AGG - chr4_3:54884211-54884230 None:intergenic 30.0%
CATTGGTATTCATCAAAAAC+TGG + chr4_3:54884211-54884230 Msa0648410:five_prime_UTR 30.0%
AGGAAACAATGTCAAGAAAA+GGG - chr4_3:54884279-54884298 None:intergenic 30.0%
AAGGAAACAATGTCAAGAAA+AGG - chr4_3:54884280-54884299 None:intergenic 30.0%
ATGAGGATGTGAAAAAATGA+AGG - chr4_3:54884299-54884318 None:intergenic 30.0%
!!! ATTTTTTCACATCCTCATGA+TGG + chr4_3:54884301-54884320 Msa0648410:five_prime_UTR 30.0%
!!! TTTTTTCACATCCTCATGAT+GGG + chr4_3:54884302-54884321 Msa0648410:five_prime_UTR 30.0%
TTACTATCCTGTTATACTCT+TGG + chr4_3:54884468-54884487 Msa0648410:five_prime_UTR 30.0%
!! TCTAGTTTCTTGACATTTTC+AGG - chr4_3:54884601-54884620 None:intergenic 30.0%
!! GTAAATTTGATTTGCCTCAA+AGG + chr4_3:54884716-54884735 Msa0648410:five_prime_UTR 30.0%
TGTTTAATAACTACCCTTTG+AGG - chr4_3:54884733-54884752 None:intergenic 30.0%
TCAAAGGGTAGTTATTAAAC+AGG + chr4_3:54884732-54884751 Msa0648410:five_prime_UTR 30.0%
CTGGTATATTGTATTCTTCT+GGG + chr4_3:54884764-54884783 Msa0648410:five_prime_UTR 30.0%
AAACCATACACATGAAACAT+TGG - chr4_3:54884919-54884938 None:intergenic 30.0%
CCTGTATAAAATCAACGTTA+GGG - chr4_3:54885194-54885213 None:intergenic 30.0%
ACCTGTATAAAATCAACGTT+AGG - chr4_3:54885195-54885214 None:intergenic 30.0%
TCAAGCAATCATATAAACGA+TGG - chr4_3:54885267-54885286 None:intergenic 30.0%
! CTGTAAGATTGTTTCCTTTT+AGG + chr4_3:54885754-54885773 Msa0648410:intron 30.0%
!!! ATTGTTTCCTTTTAGGGTTA+TGG + chr4_3:54885761-54885780 Msa0648410:intron 30.0%
TTTCACAACTATTCGTCAAT+AGG + chr4_3:54885833-54885852 Msa0648410:five_prime_UTR 30.0%
TTCACAACTATTCGTCAATA+GGG + chr4_3:54885834-54885853 Msa0648410:five_prime_UTR 30.0%
TTCTTACCTCTCATAATAGA+AGG - chr4_3:54885953-54885972 None:intergenic 30.0%
GAAACACGGTAGTAATATAA+AGG + chr4_3:54886005-54886024 Msa0648410:intron 30.0%
AACTCTTTCCTTTAATTCTC+AGG + chr4_3:54886502-54886521 Msa0648410:intron 30.0%
TCAAGTATCCTGAGAATTAA+AGG - chr4_3:54886513-54886532 None:intergenic 30.0%
CAACAATTGATAAAGATGGT+TGG + chr4_3:54886554-54886573 Msa0648410:five_prime_UTR 30.0%
TCAGGATGGATATTTACATT+TGG + chr4_3:54886606-54886625 Msa0648410:five_prime_UTR 30.0%
TATCAAATACAAGGGATTTC+AGG + chr4_3:54886648-54886667 Msa0648410:five_prime_UTR 30.0%
!! GATTTCAGGTATCTACTTTT+AGG + chr4_3:54886662-54886681 Msa0648410:intron 30.0%
TTCATATACTTACGCTGTAA+CGG - chr4_3:54886842-54886861 None:intergenic 30.0%
GAAAAATGACGTAACATTTG+AGG - chr4_3:54886954-54886973 None:intergenic 30.0%
TACCAAAGAAAGACAATACA+TGG + chr4_3:54887348-54887367 Msa0648410:three_prime_UTR 30.0%
TAGAAAGTTCAAAAACCAGA+AGG + chr4_3:54887531-54887550 Msa0648410:three_prime_UTR 30.0%
!!! TTGATGGTTTTTTTTCCTTC+TGG - chr4_3:54887549-54887568 None:intergenic 30.0%
CTCTTACTATAACTGACTAA+AGG - chr4_3:54887589-54887608 None:intergenic 30.0%
ATTTCTCTAGTGCATTTACA+AGG - chr4_3:54887614-54887633 None:intergenic 30.0%
! ATTGAAAAGTATGACACAAC+AGG - chr4_3:54887715-54887734 None:intergenic 30.0%
! TGTCATACTTTTCAATTCAG+CGG + chr4_3:54887719-54887738 Msa0648410:three_prime_UTR 30.0%
! AATTCAGCGGTTAAGATTTT+TGG + chr4_3:54887732-54887751 Msa0648410:three_prime_UTR 30.0%
!! TTAATACGTTAGTCTTCTTC+AGG + chr4_3:54887805-54887824 Msa0648410:three_prime_UTR 30.0%
CACTAATAACAGAATCATTC+AGG + chr4_3:54887866-54887885 Msa0648410:three_prime_UTR 30.0%
GCAGTGTAATAAAAAGAACA+AGG + chr4_3:54887894-54887913 Msa0648410:three_prime_UTR 30.0%
ACTTGAAACAATCAATTCTG+AGG + chr4_3:54887926-54887945 Msa0648410:three_prime_UTR 30.0%
CTTGAAACAATCAATTCTGA+GGG + chr4_3:54887927-54887946 Msa0648410:three_prime_UTR 30.0%
ACATCTGAATTATGAGTCAT+TGG + chr4_3:54887955-54887974 Msa0648410:three_prime_UTR 30.0%
!! AGTCATTGGTTTACAATACT+TGG + chr4_3:54887969-54887988 Msa0648410:three_prime_UTR 30.0%
!! TTTGTTGAAAGTGATGGATT+CGG - chr4_3:54888041-54888060 None:intergenic 30.0%
! TGTCTTTTTGTTGAAAGTGA+TGG - chr4_3:54888047-54888066 None:intergenic 30.0%
ATTATAGGATATTGGATCAG+AGG + chr4_3:54888086-54888105 Msa0648410:three_prime_UTR 30.0%
TTATAGGATATTGGATCAGA+GGG + chr4_3:54888087-54888106 Msa0648410:three_prime_UTR 30.0%
TTTGTCTTTATCATGTGTGC+GGG + chr4_3:54883785-54883804 Msa0648410:five_prime_UTR 35.0%
TGGAAATTTCTGTACGCATT+TGG - chr4_3:54883844-54883863 None:intergenic 35.0%
TTGTTGGGATCCTAAGAATA+GGG - chr4_3:54883868-54883887 None:intergenic 35.0%
TTTGTTGGGATCCTAAGAAT+AGG - chr4_3:54883869-54883888 None:intergenic 35.0%
AGGGTGAAATTGTCTTTGTT+GGG - chr4_3:54883883-54883902 None:intergenic 35.0%
AAGGGTGAAATTGTCTTTGT+TGG - chr4_3:54883884-54883903 None:intergenic 35.0%
! TGTTGGGGATTTGGAATATT+TGG - chr4_3:54883917-54883936 None:intergenic 35.0%
GAACGTAATAGCTACGTTTA+GGG - chr4_3:54883953-54883972 None:intergenic 35.0%
! GTTTTGTGTTTGTGGAAATG+GGG - chr4_3:54883986-54884005 None:intergenic 35.0%
!! GGTTTTGTGTTTGTGGAAAT+GGG - chr4_3:54883987-54884006 None:intergenic 35.0%
!! AGGTTTTGTGTTTGTGGAAA+TGG - chr4_3:54883988-54884007 None:intergenic 35.0%
!!! AAAGGGTGTTGTGTTTTTTG+AGG - chr4_3:54884028-54884047 None:intergenic 35.0%
! GAAAAAAGAGAACGGTTTTG+TGG - chr4_3:54884113-54884132 None:intergenic 35.0%
!! GTTGAGTGATCATGTTGTTT+TGG - chr4_3:54884180-54884199 None:intergenic 35.0%
! TTTTTCACATCCTCATGATG+GGG + chr4_3:54884303-54884322 Msa0648410:five_prime_UTR 35.0%
CCCAGATGAAGAATCAATAA+GGG - chr4_3:54884335-54884354 None:intergenic 35.0%
!! CCCTTATTGATTCTTCATCT+GGG + chr4_3:54884332-54884351 Msa0648410:five_prime_UTR 35.0%
ACCCAGATGAAGAATCAATA+AGG - chr4_3:54884336-54884355 None:intergenic 35.0%
GTTACCTTTAGTCAAATCCA+TGG + chr4_3:54884378-54884397 Msa0648410:five_prime_UTR 35.0%
GCTTCTGCTTTACACAAAAT+GGG + chr4_3:54884400-54884419 Msa0648410:five_prime_UTR 35.0%
ACACTACTAAGAGGATTCAA+TGG - chr4_3:54884523-54884542 None:intergenic 35.0%
TTGAATCCTCTTAGTAGTGT+TGG + chr4_3:54884523-54884542 Msa0648410:five_prime_UTR 35.0%
ATTTCACCAACACTACTAAG+AGG - chr4_3:54884532-54884551 None:intergenic 35.0%
! TGTTGGTGAAATTCGTAAAC+AGG + chr4_3:54884540-54884559 Msa0648410:five_prime_UTR 35.0%
GTCAAGAAACTAGAGCTATT+AGG + chr4_3:54884607-54884626 Msa0648410:five_prime_UTR 35.0%
AAGAAACTAGAGCTATTAGG+TGG + chr4_3:54884610-54884629 Msa0648410:five_prime_UTR 35.0%
!! TTTTCTGGCACAGCAATAAT+GGG - chr4_3:54884643-54884662 None:intergenic 35.0%
!! ATTTTCTGGCACAGCAATAA+TGG - chr4_3:54884644-54884663 None:intergenic 35.0%
! CTCAAATCCTTCTCATTTTC+TGG - chr4_3:54884658-54884677 None:intergenic 35.0%
CAGAAAATGAGAAGGATTTG+AGG + chr4_3:54884656-54884675 Msa0648410:five_prime_UTR 35.0%
ATTAAACAGGAAGATACTGC+TGG + chr4_3:54884745-54884764 Msa0648410:five_prime_UTR 35.0%
GCTGGTATATTGTATTCTTC+TGG + chr4_3:54884763-54884782 Msa0648410:five_prime_UTR 35.0%
TTGCCAATGTTTCATGTGTA+TGG + chr4_3:54884913-54884932 Msa0648410:five_prime_UTR 35.0%
GATCTACTGTCATTGTAATG+AGG + chr4_3:54884971-54884990 Msa0648410:five_prime_UTR 35.0%
CTAATAACTCCTATGCTCAA+AGG - chr4_3:54885153-54885172 None:intergenic 35.0%
TTGAGCATAGGAGTTATTAG+TGG + chr4_3:54885153-54885172 Msa0648410:five_prime_UTR 35.0%
!!! CCCTAACGTTGATTTTATAC+AGG + chr4_3:54885191-54885210 Msa0648410:five_prime_UTR 35.0%
!! GTCGTTTTCATAAGCTATCT+TGG + chr4_3:54885521-54885540 Msa0648410:intron 35.0%
! TAAGTTTTGCGAGACTGTTT+GGG - chr4_3:54885611-54885630 None:intergenic 35.0%
! ATAAGTTTTGCGAGACTGTT+TGG - chr4_3:54885612-54885631 None:intergenic 35.0%
AAACAGTCTCGCAAAACTTA+TGG + chr4_3:54885611-54885630 Msa0648410:intron 35.0%
TCACATCCACAACAAATTCT+AGG + chr4_3:54885670-54885689 Msa0648410:intron 35.0%
!! TTATGGCCTAGAATTTGTTG+TGG - chr4_3:54885679-54885698 None:intergenic 35.0%
AGTCATTCCATAACCCTAAA+AGG - chr4_3:54885771-54885790 None:intergenic 35.0%
AGCAAATATAGTAAAGGGGA+GGG - chr4_3:54886102-54886121 None:intergenic 35.0%
ATGATATTTCTCAAGGACAC+AGG - chr4_3:54886152-54886171 None:intergenic 35.0%
ATAGCGAACAACAACAATCA+AGG + chr4_3:54886324-54886343 Msa0648410:intron 35.0%
!!! AATCAAGGCCTTTTTTCACT+CGG + chr4_3:54886339-54886358 Msa0648410:intron 35.0%
! AGAAAAAAGAAGGTACAGGT+AGG - chr4_3:54886444-54886463 None:intergenic 35.0%
! GACAAGAAAAAAGAAGGTAC+AGG - chr4_3:54886448-54886467 None:intergenic 35.0%
CAGCAAGACAAGAAAAAAGA+AGG - chr4_3:54886454-54886473 None:intergenic 35.0%
CTCAGGATACTTGAATAATG+AGG + chr4_3:54886519-54886538 Msa0648410:intron 35.0%
! GCTGCATTTTCTATAGATTG+CGG - chr4_3:54886887-54886906 None:intergenic 35.0%
!! AGAGGCACTTTAGAAGATTA+CGG - chr4_3:54886913-54886932 None:intergenic 35.0%
!! AATCTTCTAAAGTGCCTCTT+TGG + chr4_3:54886914-54886933 Msa0648410:CDS 35.0%
TTCCCCAATTTCTTCATCTT+CGG - chr4_3:54886987-54887006 None:intergenic 35.0%
!! TGCTTGAGTTCTAAACTCTA+AGG + chr4_3:54887296-54887315 Msa0648410:CDS 35.0%
CAAAGAAAGACAATACATGG+CGG + chr4_3:54887351-54887370 Msa0648410:three_prime_UTR 35.0%
AAAGAAAGACAATACATGGC+GGG + chr4_3:54887352-54887371 Msa0648410:three_prime_UTR 35.0%
TTACCATAACATGAAACTGG+TGG + chr4_3:54887375-54887394 Msa0648410:three_prime_UTR 35.0%
! TAACTGACTAAAGGTGTACA+GGG - chr4_3:54887580-54887599 None:intergenic 35.0%
! ATAACTGACTAAAGGTGTAC+AGG - chr4_3:54887581-54887600 None:intergenic 35.0%
!!! TTAAGATTTTTGGTGGTCGA+AGG + chr4_3:54887742-54887761 Msa0648410:three_prime_UTR 35.0%
AACTGCGTTCAAATTAGCAA+AGG + chr4_3:54888009-54888028 Msa0648410:three_prime_UTR 35.0%
CACATCTCATTATGTTTGAG+AGG + chr4_3:54888121-54888140 Msa0648410:three_prime_UTR 35.0%
CTACATGAGACTTATGGTAA+TGG - chr4_3:54888200-54888219 None:intergenic 35.0%
ACCATAAGTCTCATGTAGAT+TGG + chr4_3:54888202-54888221 Msa0648410:three_prime_UTR 35.0%
CCATAAGTCTCATGTAGATT+GGG + chr4_3:54888203-54888222 Msa0648410:three_prime_UTR 35.0%
GGAACGTAATAGCTACGTTT+AGG - chr4_3:54883954-54883973 None:intergenic 40.0%
GTGGAAATGGGGATATGAAA+GGG - chr4_3:54883975-54883994 None:intergenic 40.0%
TGTGGAAATGGGGATATGAA+AGG - chr4_3:54883976-54883995 None:intergenic 40.0%
!! GTGTGAAGGTTTTGTGTTTG+TGG - chr4_3:54883994-54884013 None:intergenic 40.0%
!!! TGTTGTGTTTTTTGAGGCTG+TGG - chr4_3:54884022-54884041 None:intergenic 40.0%
ATGAATACCAATGAGGGTGA+GGG - chr4_3:54884204-54884223 None:intergenic 40.0%
ATGTCAAGAAAAGGGTCAGT+TGG - chr4_3:54884271-54884290 None:intergenic 40.0%
!! GCCCTTATTGATTCTTCATC+TGG + chr4_3:54884331-54884350 Msa0648410:five_prime_UTR 40.0%
GAAGCCATGGATTTGACTAA+AGG - chr4_3:54884385-54884404 None:intergenic 40.0%
TTTGTGTAAAGCAGAAGCCA+TGG - chr4_3:54884398-54884417 None:intergenic 40.0%
GGCTTCTGCTTTACACAAAA+TGG + chr4_3:54884399-54884418 Msa0648410:five_prime_UTR 40.0%
CACAAAATGGGTGTTTCACA+AGG + chr4_3:54884412-54884431 Msa0648410:five_prime_UTR 40.0%
! AGAACAGCCAAGAGTATAAC+AGG - chr4_3:54884478-54884497 None:intergenic 40.0%
CTCTTGGCTGTTCTGTATTT+AGG + chr4_3:54884484-54884503 Msa0648410:five_prime_UTR 40.0%
!! TTGTATTCTTCTGGGACTAC+AGG + chr4_3:54884772-54884791 Msa0648410:five_prime_UTR 40.0%
AACAGCTAGAAACACACTCT+TGG - chr4_3:54884893-54884912 None:intergenic 40.0%
GAGGAAATTTGACATTGACG+AGG + chr4_3:54884990-54885009 Msa0648410:five_prime_UTR 40.0%
TTGACATTGACGAGGTTATC+AGG + chr4_3:54884998-54885017 Msa0648410:five_prime_UTR 40.0%
TGACATTGACGAGGTTATCA+GGG + chr4_3:54884999-54885018 Msa0648410:five_prime_UTR 40.0%
ATTGTCAATGCCGACAACAT+CGG - chr4_3:54885066-54885085 None:intergenic 40.0%
! GCATTGACAATGAAGGCAAA+GGG + chr4_3:54885075-54885094 Msa0648410:five_prime_UTR 40.0%
!! ATGAAGGCAAAGGGTGTTAA+TGG + chr4_3:54885084-54885103 Msa0648410:five_prime_UTR 40.0%
! TAAGTTGCAGAGTTTGAGAC+AGG + chr4_3:54885110-54885129 Msa0648410:five_prime_UTR 40.0%
AATCAACGTTAGGGAAAGCA+TGG - chr4_3:54885185-54885204 None:intergenic 40.0%
ACTGTTATATAGGGCCTGTT+TGG + chr4_3:54885388-54885407 Msa0648410:intron 40.0%
GTTGTGGATGTGAACATCAA+TGG - chr4_3:54885663-54885682 None:intergenic 40.0%
CTATTCGTCAATAGGGTTGT+TGG + chr4_3:54885841-54885860 Msa0648410:five_prime_UTR 40.0%
AAAGTGGTAGACTGGAACAA+TGG + chr4_3:54885881-54885900 Msa0648410:five_prime_UTR 40.0%
!!! AATGGTACTTTTTTACCCCC+AGG + chr4_3:54885899-54885918 Msa0648410:five_prime_UTR 40.0%
GAGCAAATATAGTAAAGGGG+AGG - chr4_3:54886103-54886122 None:intergenic 40.0%
! TTTTCCCACCGAGTGAAAAA+AGG - chr4_3:54886350-54886369 None:intergenic 40.0%
TACAGGTAGGCTAAGGAATA+AGG - chr4_3:54886431-54886450 None:intergenic 40.0%
! AAAGATGGTTGGCTACATAC+AGG + chr4_3:54886565-54886584 Msa0648410:five_prime_UTR 40.0%
! GCACTTTAGAAGATTACGGA+TGG - chr4_3:54886909-54886928 None:intergenic 40.0%
! AGTGCCGAAGATGAAGAAAT+TGG + chr4_3:54886980-54886999 Msa0648410:CDS 40.0%
GTGCCGAAGATGAAGAAATT+GGG + chr4_3:54886981-54887000 Msa0648410:CDS 40.0%
TGCCGAAGATGAAGAAATTG+GGG + chr4_3:54886982-54887001 Msa0648410:CDS 40.0%
AGAAATTGGGGAAATTCCAG+TGG + chr4_3:54886994-54887013 Msa0648410:CDS 40.0%
TTCTTGACAACAAATGCCAC+TGG - chr4_3:54887013-54887032 None:intergenic 40.0%
!! AGTGGCATTTGTTGTCAAGA+AGG + chr4_3:54887012-54887031 Msa0648410:CDS 40.0%
!! GCATTTGTTGTCAAGAAGGT+TGG + chr4_3:54887016-54887035 Msa0648410:CDS 40.0%
ACATAATCGACCACATGCTT+CGG - chr4_3:54887055-54887074 None:intergenic 40.0%
! GAGCAGGTTCTTCTTTTGAT+TGG + chr4_3:54887079-54887098 Msa0648410:intron 40.0%
CGCCATGTATTGTCTTTCTT+TGG - chr4_3:54887353-54887372 None:intergenic 40.0%
GGGTTACCATAACATGAAAC+TGG + chr4_3:54887372-54887391 Msa0648410:three_prime_UTR 40.0%
AGTCCACCAGTTTCATGTTA+TGG - chr4_3:54887381-54887400 None:intergenic 40.0%
!! TCAGCGGTTAAGATTTTTGG+TGG + chr4_3:54887735-54887754 Msa0648410:three_prime_UTR 40.0%
! GTTCAAATTAGCAAAGGTGC+AGG + chr4_3:54888015-54888034 Msa0648410:three_prime_UTR 40.0%
CCCAATCTACATGAGACTTA+TGG - chr4_3:54888206-54888225 None:intergenic 40.0%
! ACTTGTCGGTTGTGCCTTAA+AGG - chr4_3:54883457-54883476 None:intergenic 45.0%
!! ACGCATTTGGCTCTCAAGTT+GGG - chr4_3:54883831-54883850 None:intergenic 45.0%
!! TACGCATTTGGCTCTCAAGT+TGG - chr4_3:54883832-54883851 None:intergenic 45.0%
TGGGATCCTAAGAATAGGGT+TGG - chr4_3:54883864-54883883 None:intergenic 45.0%
!! GTGGTGTGAAGTGTTGAATG+TGG - chr4_3:54884094-54884113 None:intergenic 45.0%
GATGAATACCAATGAGGGTG+AGG - chr4_3:54884205-54884224 None:intergenic 45.0%
CAAGAAAAGGGTCAGTTGGA+AGG - chr4_3:54884267-54884286 None:intergenic 45.0%
CGTAAACAGGTTCAAGAGTG+TGG + chr4_3:54884553-54884572 Msa0648410:five_prime_UTR 45.0%
TGCTGTGCCAGAAAATGAGA+AGG + chr4_3:54884648-54884667 Msa0648410:five_prime_UTR 45.0%
TGGTTTATCGCTCTTTGCTG+CGG + chr4_3:54884933-54884952 Msa0648410:five_prime_UTR 45.0%
TGTTGTTCCACCGATGTTGT+CGG + chr4_3:54885053-54885072 Msa0648410:five_prime_UTR 45.0%
!! GTTGTCGGCATTGACAATGA+AGG + chr4_3:54885068-54885087 Msa0648410:five_prime_UTR 45.0%
!! GGCATTGACAATGAAGGCAA+AGG + chr4_3:54885074-54885093 Msa0648410:five_prime_UTR 45.0%
! AGTTTGAGACAGGTCTCTTG+CGG + chr4_3:54885120-54885139 Msa0648410:five_prime_UTR 45.0%
AGAGGGCCTTCTATTATGAG+AGG + chr4_3:54885944-54885963 Msa0648410:five_prime_UTR 45.0%
!! AAAGGTTTGAGTTGCTGCAG+CGG - chr4_3:54886068-54886087 None:intergenic 45.0%
GGGAGGGAGTCTTGATATAA+AGG - chr4_3:54886086-54886105 None:intergenic 45.0%
!!! AAGGCCTTTTTTCACTCGGT+GGG + chr4_3:54886343-54886362 Msa0648410:intron 45.0%
! AAGAAGGTACAGGTAGGCTA+AGG - chr4_3:54886438-54886457 None:intergenic 45.0%
AACACAGCTTCTAGATCAGC+AGG - chr4_3:54886790-54886809 None:intergenic 45.0%
!! CTTCATCTTCGGCACTGATA+TGG - chr4_3:54886976-54886995 None:intergenic 45.0%
TGTACTTTCTCCGAAGCATG+TGG + chr4_3:54887042-54887061 Msa0648410:CDS 45.0%
TTGCTCCATACAAGAAGGTC+CGG + chr4_3:54887204-54887223 Msa0648410:CDS 45.0%
TCTTCACCGACAAGATACCA+AGG + chr4_3:54887234-54887253 Msa0648410:CDS 45.0%
GTACAGGGCAGAGTAATTGA+TGG - chr4_3:54887565-54887584 None:intergenic 45.0%
!! AAGAAAAAAAATAAAATAAA+GGG - chr4_3:54887176-54887195 None:intergenic 5.0%
GGGTCAGTTGGAAGGTTTCT+AGG - chr4_3:54884259-54884278 None:intergenic 50.0%
AGGGCTAAAACCCCATCATG+AGG - chr4_3:54884316-54884335 None:intergenic 50.0%
GGGACTACAGGTGTGAGTAA+AGG + chr4_3:54884784-54884803 Msa0648410:five_prime_UTR 50.0%
GGTTTATCGCTCTTTGCTGC+GGG + chr4_3:54884934-54884953 Msa0648410:five_prime_UTR 50.0%
TGCTGCGGGATTATTGTCGT+TGG + chr4_3:54884948-54884967 Msa0648410:five_prime_UTR 50.0%
GCTGCGGGATTATTGTCGTT+GGG + chr4_3:54884949-54884968 Msa0648410:five_prime_UTR 50.0%
GACAACATCGGTGGAACAAC+AGG - chr4_3:54885054-54885073 None:intergenic 50.0%
AATGACTGAGTCGACTGCAG+TGG + chr4_3:54885784-54885803 Msa0648410:five_prime_UTR 50.0%
ATGACTGAGTCGACTGCAGT+GGG + chr4_3:54885785-54885804 Msa0648410:five_prime_UTR 50.0%
!! AGGGTTGTTGGCTCCAAACA+CGG + chr4_3:54885853-54885872 Msa0648410:five_prime_UTR 50.0%
TCCAAACACGGAGGCAAAAG+TGG + chr4_3:54885865-54885884 Msa0648410:five_prime_UTR 50.0%
!! ACCACTTTTGCCTCCGTGTT+TGG - chr4_3:54885869-54885888 None:intergenic 50.0%
!!! CAAGGCCTTTTTTCACTCGG+TGG + chr4_3:54886342-54886361 Msa0648410:intron 50.0%
!! ACGGCTGCATCGACTATTTC+AGG - chr4_3:54886823-54886842 None:intergenic 50.0%
GGTCGATTATGTTGCTGAGC+AGG + chr4_3:54887063-54887082 Msa0648410:CDS 50.0%
GCAGGTTGCTCCATACAAGA+AGG + chr4_3:54887199-54887218 Msa0648410:intron 50.0%
TCCATACAAGAAGGTCCGGA+AGG + chr4_3:54887208-54887227 Msa0648410:CDS 50.0%
ACCTTCCGGACCTTCTTGTA+TGG - chr4_3:54887212-54887231 None:intergenic 50.0%
TGTCGGTGAAGAACACCTTC+CGG - chr4_3:54887226-54887245 None:intergenic 50.0%
CGGAGACCTTGGTATCTTGT+CGG - chr4_3:54887243-54887262 None:intergenic 50.0%
AAGATACCAAGGTCTCCGAC+TGG + chr4_3:54887245-54887264 Msa0648410:CDS 50.0%
AGATACCAAGGTCTCCGACT+GGG + chr4_3:54887246-54887265 Msa0648410:CDS 50.0%
TTTCGGAGGATCTTCCCAGT+CGG - chr4_3:54887263-54887282 None:intergenic 50.0%
AGATCCTCCGAAAGCAACTC+AGG + chr4_3:54887270-54887289 Msa0648410:CDS 50.0%
! AGCAGTGCCTGAGTTGCTTT+CGG - chr4_3:54887280-54887299 None:intergenic 50.0%
AGGCTGTGGCTTGAGTGTGA+AGG - chr4_3:54884008-54884027 None:intergenic 55.0%
CTCAACACCCTCACCCTCAT+TGG + chr4_3:54884194-54884213 Msa0648410:five_prime_UTR 55.0%
GTCAATGCCGACAACATCGG+TGG - chr4_3:54885063-54885082 None:intergenic 55.0%
!! GTTGTTGGCTCCAAACACGG+AGG + chr4_3:54885856-54885875 Msa0648410:five_prime_UTR 55.0%
CGGAGGCAAAAGTGGTAGAC+TGG + chr4_3:54885873-54885892 Msa0648410:five_prime_UTR 55.0%
! TTTTTACCCCCAGGCAGCTG+CGG + chr4_3:54885908-54885927 Msa0648410:five_prime_UTR 55.0%
! TGCGGTGAGCTTTGGTTGAG+AGG + chr4_3:54885926-54885945 Msa0648410:five_prime_UTR 55.0%
! GCGGTGAGCTTTGGTTGAGA+GGG + chr4_3:54885927-54885946 Msa0648410:five_prime_UTR 55.0%
CAGCGGTGTGACTAACGTTC+AGG - chr4_3:54886051-54886070 None:intergenic 55.0%
ATCTTCCCAGTCGGAGACCT+TGG - chr4_3:54887254-54887273 None:intergenic 55.0%
! AGTGCCTGAGTTGCTTTCGG+AGG - chr4_3:54887277-54887296 None:intergenic 55.0%
GGTGCAGCGCCTTTGAGCAT+AGG + chr4_3:54885141-54885160 Msa0648410:five_prime_UTR 60.0%
TCGACTGCAGTGGGAACACG+TGG + chr4_3:54885794-54885813 Msa0648410:five_prime_UTR 60.0%
AAAGCTCACCGCAGCTGCCT+GGG - chr4_3:54885919-54885938 None:intergenic 60.0%
AAGCTCACCGCAGCTGCCTG+GGG - chr4_3:54885918-54885937 None:intergenic 65.0%
CAAAGCTCACCGCAGCTGCC+TGG - chr4_3:54885920-54885939 None:intergenic 65.0%
CAGGCAGCTGCGGTGAGCTT+TGG + chr4_3:54885918-54885937 Msa0648410:five_prime_UTR 65.0%
AGCTCACCGCAGCTGCCTGG+GGG - chr4_3:54885917-54885936 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 54883285 54888300 54883285 ID=Msa0648410;Name=Msa0648410
chr4_3 mRNA 54883285 54888300 54883285 ID=Msa0648410-mRNA-1;Parent=Msa0648410;Name=Msa0648410-mRNA-1;_AED=0.03;_eAED=0.03;_QI=2316|1|1|1|1|0.8|5|984|121
chr4_3 exon 54883285 54885212 54883285 ID=Msa0648410-mRNA-1:exon:13888;Parent=Msa0648410-mRNA-1
chr4_3 exon 54885776 54885965 54885776 ID=Msa0648410-mRNA-1:exon:13889;Parent=Msa0648410-mRNA-1
chr4_3 exon 54886524 54886669 54886524 ID=Msa0648410-mRNA-1:exon:13890;Parent=Msa0648410-mRNA-1
chr4_3 exon 54886781 54887084 54886781 ID=Msa0648410-mRNA-1:exon:13891;Parent=Msa0648410-mRNA-1
chr4_3 exon 54887203 54888300 54887203 ID=Msa0648410-mRNA-1:exon:13892;Parent=Msa0648410-mRNA-1
chr4_3 five_prime_UTR 54883285 54885212 54883285 ID=Msa0648410-mRNA-1:five_prime_utr;Parent=Msa0648410-mRNA-1
chr4_3 five_prime_UTR 54885776 54885965 54885776 ID=Msa0648410-mRNA-1:five_prime_utr;Parent=Msa0648410-mRNA-1
chr4_3 five_prime_UTR 54886524 54886669 54886524 ID=Msa0648410-mRNA-1:five_prime_utr;Parent=Msa0648410-mRNA-1
chr4_3 five_prime_UTR 54886781 54886832 54886781 ID=Msa0648410-mRNA-1:five_prime_utr;Parent=Msa0648410-mRNA-1
chr4_3 CDS 54886833 54887084 54886833 ID=Msa0648410-mRNA-1:cds;Parent=Msa0648410-mRNA-1
chr4_3 CDS 54887203 54887316 54887203 ID=Msa0648410-mRNA-1:cds;Parent=Msa0648410-mRNA-1
chr4_3 three_prime_UTR 54887317 54888300 54887317 ID=Msa0648410-mRNA-1:three_prime_utr;Parent=Msa0648410-mRNA-1
Gene Sequence

>Msa0648410

ATGCAGCCGTTACAGCGTAAGTATATGAACACTTCATTTATGTCTTCGCTTCCGCAATCTATAGAAAATGCAGCCATCCGTAATCTTCTAAAGTGCCTCTTTGGACATTTGATTATAACCTCAAATGTTACGTCATTTTTCCATATCAGTGCCGAAGATGAAGAAATTGGGGAAATTCCAGTGGCATTTGTTGTCAAGAAGGTTGGAAGTGTACTTTCTCCGAAGCATGTGGTCGATTATGTTGCTGAGCAGGTTGCTCCATACAAGAAGGTCCGGAAGGTGTTCTTCACCGACAAGATACCAAGGTCTCCGACTGGGAAGATCCTCCGAAAGCAACTCAGGCACTGCTTGAGTTCTAAACTCTAA
Protein sequence

>Msa0648410

MQPLQRKYMNTSFMSSLPQSIENAAIRNLLKCLFGHLIITSNVTSFFHISAEDEEIGEIPVAFVVKKVGSVLSPKHVVDYVAEQVAPYKKVRKVFFTDKIPRSPTGKILRKQLRHCLSSKL*