AlfalfaGEDB | MS.gene89488

M. sativa cultivar XinJiangDaYe / MS.gene89488

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89488.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89488.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89488.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene89488	0.81171	6.11E-51	-1.69E-46
MS.gene049159	MS.gene89488	0.818952	1.50E-52	-1.69E-46
MS.gene049341	MS.gene89488	0.804508	2.09E-49	-1.69E-46
MS.gene049350	MS.gene89488	0.802864	4.58E-49	-1.69E-46
MS.gene049368	MS.gene89488	0.819243	1.29E-52	-1.69E-46
MS.gene049821	MS.gene89488	0.880751	4.24E-70	-1.69E-46
MS.gene050020	MS.gene89488	0.819417	1.17E-52	-1.69E-46
MS.gene050253	MS.gene89488	0.802348	5.86E-49	-1.69E-46
MS.gene050689	MS.gene89488	0.806883	6.63E-50	-1.69E-46
MS.gene050807	MS.gene89488	0.859602	3.69E-63	-1.69E-46
MS.gene050808	MS.gene89488	0.823606	1.27E-53	-1.69E-46
MS.gene050815	MS.gene89488	0.810005	1.43E-50	-1.69E-46
MS.gene050838	MS.gene89488	0.813741	2.19E-51	-1.69E-46
MS.gene050901	MS.gene89488	0.839524	1.51E-57	-1.69E-46
MS.gene051102	MS.gene89488	0.80396	2.72E-49	-1.69E-46
MS.gene051243	MS.gene89488	0.80377	2.97E-49	-1.69E-46
MS.gene051247	MS.gene89488	0.812294	4.56E-51	-1.69E-46
MS.gene051328	MS.gene89488	0.809507	1.83E-50	-1.69E-46
MS.gene05157	MS.gene89488	0.807662	4.53E-50	-1.69E-46
MS.gene051676	MS.gene89488	0.80421	2.41E-49	-1.69E-46
MS.gene051700	MS.gene89488	0.811494	6.81E-51	-1.69E-46
MS.gene051719	MS.gene89488	0.817344	3.46E-52	-1.69E-46
MS.gene051720	MS.gene89488	0.831903	1.28E-55	-1.69E-46
MS.gene051975	MS.gene89488	0.80407	2.58E-49	-1.69E-46
MS.gene052133	MS.gene89488	0.834116	3.61E-56	-1.69E-46
MS.gene052397	MS.gene89488	0.808772	2.63E-50	-1.69E-46
MS.gene052516	MS.gene89488	0.815284	1.00E-51	-1.69E-46
MS.gene052665	MS.gene89488	0.831702	1.44E-55	-1.69E-46
MS.gene053008	MS.gene89488	0.809351	1.98E-50	-1.69E-46
MS.gene053058	MS.gene89488	0.803602	3.22E-49	-1.69E-46
MS.gene053563	MS.gene89488	0.842503	2.48E-58	-1.69E-46
MS.gene053581	MS.gene89488	0.81944	1.16E-52	-1.69E-46
MS.gene05364	MS.gene89488	0.826017	3.42E-54	-1.69E-46
MS.gene05517	MS.gene89488	0.816745	4.72E-52	-1.69E-46
MS.gene055311	MS.gene89488	0.825365	4.88E-54	-1.69E-46
MS.gene055313	MS.gene89488	0.825022	5.88E-54	-1.69E-46
MS.gene055314	MS.gene89488	0.802948	4.40E-49	-1.69E-46
MS.gene055465	MS.gene89488	0.834927	2.26E-56	-1.69E-46
MS.gene055466	MS.gene89488	0.829033	6.46E-55	-1.69E-46
MS.gene055468	MS.gene89488	0.823848	1.11E-53	-1.69E-46
MS.gene055469	MS.gene89488	0.829548	4.84E-55	-1.69E-46
MS.gene055513	MS.gene89488	0.813558	2.41E-51	-1.69E-46
MS.gene055623	MS.gene89488	0.812454	4.21E-51	-1.69E-46
MS.gene055926	MS.gene89488	0.814441	1.54E-51	-1.69E-46
MS.gene056005	MS.gene89488	0.819787	9.67E-53	-1.69E-46
MS.gene05604	MS.gene89488	0.815636	8.35E-52	-1.69E-46
MS.gene056215	MS.gene89488	0.818374	2.03E-52	-1.69E-46
MS.gene056308	MS.gene89488	0.829022	6.49E-55	-1.69E-46
MS.gene056760	MS.gene89488	0.815173	1.06E-51	-1.69E-46
MS.gene057693	MS.gene89488	0.815355	9.64E-52	-1.69E-46
MS.gene057915	MS.gene89488	0.810482	1.13E-50	-1.69E-46
MS.gene05847	MS.gene89488	0.805399	1.36E-49	-1.69E-46
MS.gene058639	MS.gene89488	0.803923	2.76E-49	-1.69E-46
MS.gene058852	MS.gene89488	0.812084	5.06E-51	-1.69E-46
MS.gene059171	MS.gene89488	0.801436	9.01E-49	-1.69E-46
MS.gene059725	MS.gene89488	0.833964	3.94E-56	-1.69E-46
MS.gene05978	MS.gene89488	0.808018	3.81E-50	-1.69E-46
MS.gene059842	MS.gene89488	0.800813	1.21E-48	-1.69E-46
MS.gene060365	MS.gene89488	0.801821	7.51E-49	-1.69E-46
MS.gene060429	MS.gene89488	0.803067	4.16E-49	-1.69E-46
MS.gene060587	MS.gene89488	0.814299	1.65E-51	-1.69E-46
MS.gene060803	MS.gene89488	0.812503	4.10E-51	-1.69E-46
MS.gene060998	MS.gene89488	0.800084	1.70E-48	-1.69E-46
MS.gene061006	MS.gene89488	0.804985	1.66E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene04452	MS.gene89488	PPI
MS.gene89488	MS.gene018578	PPI
MS.gene64650	MS.gene89488	PPI
MS.gene049705	MS.gene89488	PPI
MS.gene035787	MS.gene89488	PPI
MS.gene89488	MS.gene018580	PPI
MS.gene89488	MS.gene004124	PPI
MS.gene09871	MS.gene89488	PPI
MS.gene04576	MS.gene89488	PPI
MS.gene89487	MS.gene89488	PPI
MS.gene016624	MS.gene89488	PPI
MS.gene04392	MS.gene89488	PPI
MS.gene89488	MS.gene64650	PPI
MS.gene30920	MS.gene89488	PPI
MS.gene08053	MS.gene89488	PPI
MS.gene061872	MS.gene89488	PPI
MS.gene89488	MS.gene061874	PPI
MS.gene04068	MS.gene89488	PPI
MS.gene070241	MS.gene89488	PPI
MS.gene04434	MS.gene89488	PPI
MS.gene022946	MS.gene89488	PPI
MS.gene018580	MS.gene89488	PPI
MS.gene89488	MS.gene004121	PPI
MS.gene04064	MS.gene89488	PPI
MS.gene061876	MS.gene89488	PPI
MS.gene05619	MS.gene89488	PPI
MS.gene05608	MS.gene89488	PPI
MS.gene022932	MS.gene89488	PPI
MS.gene012391	MS.gene89488	PPI
MS.gene88681	MS.gene89488	PPI
MS.gene070244	MS.gene89488	PPI
MS.gene035897	MS.gene89488	PPI
MS.gene64920	MS.gene89488	PPI
MS.gene060463	MS.gene89488	PPI
MS.gene070907	MS.gene89488	PPI
MS.gene60093	MS.gene89488	PPI
MS.gene004124	MS.gene89488	PPI
MS.gene018578	MS.gene89488	PPI
MS.gene31196	MS.gene89488	PPI
MS.gene010219	MS.gene89488	PPI
MS.gene060465	MS.gene89488	PPI
MS.gene061867	MS.gene89488	PPI
MS.gene08121	MS.gene89488	PPI
MS.gene89488	MS.gene68545	PPI
MS.gene89488	MS.gene016624	PPI
MS.gene035718	MS.gene89488	PPI
MS.gene035896	MS.gene89488	PPI
MS.gene008276	MS.gene89488	PPI
MS.gene009027	MS.gene89488	PPI
MS.gene09873	MS.gene89488	PPI
MS.gene78578	MS.gene89488	PPI
MS.gene035721	MS.gene89488	PPI
MS.gene04052	MS.gene89488	PPI
MS.gene08122	MS.gene89488	PPI
MS.gene048705	MS.gene89488	PPI
MS.gene05623	MS.gene89488	PPI
MS.gene043967	MS.gene89488	PPI
MS.gene84536	MS.gene89488	PPI
MS.gene04972	MS.gene89488	PPI
MS.gene028629	MS.gene89488	PPI
MS.gene82546	MS.gene89488	PPI
MS.gene73136	MS.gene89488	PPI
MS.gene61017	MS.gene89488	PPI
MS.gene04433	MS.gene89488	PPI
MS.gene89488	MS.gene049705	PPI
MS.gene04067	MS.gene89488	PPI
MS.gene71215	MS.gene89488	PPI
MS.gene061874	MS.gene89488	PPI
MS.gene061866	MS.gene89488	PPI
MS.gene061870	MS.gene89488	PPI
MS.gene004121	MS.gene89488	PPI
MS.gene035719	MS.gene89488	PPI
MS.gene58854	MS.gene89488	PPI
MS.gene66225	MS.gene89488	PPI
MS.gene70955	MS.gene89488	PPI
MS.gene071136	MS.gene89488	PPI
MS.gene040383	MS.gene89488	PPI
MS.gene070242	MS.gene89488	PPI
MS.gene072493	MS.gene89488	PPI
MS.gene035720	MS.gene89488	PPI
MS.gene012390	MS.gene89488	PPI
MS.gene048746	MS.gene89488	PPI
MS.gene010220	MS.gene89488	PPI
MS.gene89488	MS.gene60093	PPI
MS.gene79195	MS.gene89488	PPI
MS.gene88680	MS.gene89488	PPI
MS.gene68545	MS.gene89488	PPI
MS.gene056215	MS.gene89488	PPI
MS.gene061869	MS.gene89488	PPI
MS.gene064557	MS.gene89488	PPI
MS.gene049821	MS.gene89488	PPI
MS.gene62520	MS.gene89488	PPI
MS.gene038469	MS.gene89488	PPI
MS.gene89488	MS.gene04972	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89488.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89488.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89488.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89488.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89488.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89488.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89488.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89488.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89488.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89488.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89488.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89488.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89488.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89488.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene89488.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene89488.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89488.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene89488.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene89488.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene89488.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene89488.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene89488.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene89488.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene89488.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene89488.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene89488.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene89488.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 27 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAAGAACCCTTTATGGTTT+TGG	0.212027	5.4:-49488546	MS.gene89488:CDS
AGGCAAGGAAGAACCCTTTA+TGG	0.295802	5.4:-49488552	MS.gene89488:CDS
AGGCAAAGGAGGAAAGGGTC+TGG	0.443290	5.4:-49488817	MS.gene89488:CDS
TCGCAAAACGGTGACTGCTA+TGG	0.458269	5.4:-49488595	MS.gene89488:CDS
GAGACGACGAATCGCAGGTT+TGG	0.461583	5.4:+49488737	None:intergenic
ATCTTGCAATCAACAATGTC+AGG	0.473641	5.4:-49488843	None:intergenic
GGAAAGGGTCTGGGAAAGGG+TGG	0.488132	5.4:-49488807	MS.gene89488:CDS
GGCAAAGGAGGAAAGGGTCT+GGG	0.493919	5.4:-49488816	MS.gene89488:CDS
GGAGGAAAGGGTCTGGGAAA+GGG	0.501688	5.4:-49488810	MS.gene89488:CDS
AGGAGGAAAGGGTCTGGGAA+AGG	0.504074	5.4:-49488811	MS.gene89488:CDS
AGGAAGAGGCAAAGGAGGAA+AGG	0.518412	5.4:-49488823	MS.gene89488:CDS
GTTCTGAGGGATAACATTCA+AGG	0.519054	5.4:-49488765	MS.gene89488:CDS
GGAAGAGGCAAAGGAGGAAA+GGG	0.527098	5.4:-49488822	MS.gene89488:CDS
CTGCGATTCGTCGTCTCGCA+AGG	0.531517	5.4:-49488731	MS.gene89488:CDS
CTTGCGAGACGACGAATCGC+AGG	0.555283	5.4:+49488732	None:intergenic
TGAACATGCTCGTCGCAAAA+CGG	0.556730	5.4:-49488607	MS.gene89488:CDS
CAATCAACAATGTCAGGAAG+AGG	0.577531	5.4:-49488837	None:intergenic
AGCGTCATCGCAAAGTTCTG+AGG	0.579604	5.4:-49488779	MS.gene89488:CDS
ACAATGTCAGGAAGAGGCAA+AGG	0.598135	5.4:-49488831	MS.gene89488:CDS
GTTTATGCACTCAAGAGGCA+AGG	0.602465	5.4:-49488567	MS.gene89488:CDS
ATGTCAGGAAGAGGCAAAGG+AGG	0.604597	5.4:-49488828	MS.gene89488:CDS
ATTCGTCGTCTCGCAAGGCG+TGG	0.608390	5.4:-49488726	MS.gene89488:CDS
GCGTCATCGCAAAGTTCTGA+GGG	0.612354	5.4:-49488778	MS.gene89488:CDS
ATGTTGTTTATGCACTCAAG+AGG	0.622074	5.4:-49488572	MS.gene89488:CDS
CGTCGTCTCGCAAGGCGTGG+TGG	0.632134	5.4:-49488723	MS.gene89488:CDS
GGTGGTGTGAAGCGTATCAG+TGG	0.661880	5.4:-49488705	MS.gene89488:CDS
CTGATCTATGAAGAGACTCG+CGG	0.699307	5.4:-49488681	MS.gene89488:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATGTTGTTTATGCACTCAAG+AGG	-	chr5.4:49488795-49488814	MS.gene89488:CDS	35.0%
!	GAAGAACCCTTTATGGTTTT+GGG	-	chr5.4:49488822-49488841	MS.gene89488:CDS	35.0%
!!	AAGAACCCTTTATGGTTTTG+GGG	-	chr5.4:49488823-49488842	MS.gene89488:CDS	35.0%
	CTAACCCCCAAAACCATAAA+GGG	+	chr5.4:49488831-49488850	None:intergenic	40.0%
!	GGAAGAACCCTTTATGGTTT+TGG	-	chr5.4:49488821-49488840	MS.gene89488:CDS	40.0%
!!	GTTCTGAGGGATAACATTCA+AGG	-	chr5.4:49488602-49488621	MS.gene89488:CDS	40.0%
!!!	AGAACCCTTTATGGTTTTGG+GGG	-	chr5.4:49488824-49488843	MS.gene89488:CDS	40.0%
	AGGCAAGGAAGAACCCTTTA+TGG	-	chr5.4:49488815-49488834	MS.gene89488:CDS	45.0%
	CTGATCTATGAAGAGACTCG+CGG	-	chr5.4:49488686-49488705	MS.gene89488:CDS	45.0%
	GTTTATGCACTCAAGAGGCA+AGG	-	chr5.4:49488800-49488819	MS.gene89488:CDS	45.0%
	TGAACATGCTCGTCGCAAAA+CGG	-	chr5.4:49488760-49488779	MS.gene89488:CDS	45.0%
	AGCGTCATCGCAAAGTTCTG+AGG	-	chr5.4:49488588-49488607	MS.gene89488:CDS	50.0%
	AGGAAGAGGCAAAGGAGGAA+AGG	-	chr5.4:49488544-49488563	MS.gene89488:CDS	50.0%
	ATGTCAGGAAGAGGCAAAGG+AGG	-	chr5.4:49488539-49488558	MS.gene89488:CDS	50.0%
	GGAAGAGGCAAAGGAGGAAA+GGG	-	chr5.4:49488545-49488564	MS.gene89488:CDS	50.0%
	TCGCAAAACGGTGACTGCTA+TGG	-	chr5.4:49488772-49488791	MS.gene89488:CDS	50.0%
!	GCGTCATCGCAAAGTTCTGA+GGG	-	chr5.4:49488589-49488608	MS.gene89488:CDS	50.0%
	AGGAGGAAAGGGTCTGGGAA+AGG	-	chr5.4:49488556-49488575	MS.gene89488:CDS	55.0%
	AGGCAAAGGAGGAAAGGGTC+TGG	-	chr5.4:49488550-49488569	MS.gene89488:CDS	55.0%
	GAGACGACGAATCGCAGGTT+TGG	+	chr5.4:49488633-49488652	None:intergenic	55.0%
	GGAGGAAAGGGTCTGGGAAA+GGG	-	chr5.4:49488557-49488576	MS.gene89488:CDS	55.0%
	GGCAAAGGAGGAAAGGGTCT+GGG	-	chr5.4:49488551-49488570	MS.gene89488:CDS	55.0%
	GGTGGTGTGAAGCGTATCAG+TGG	-	chr5.4:49488662-49488681	MS.gene89488:CDS	55.0%
	ATTCGTCGTCTCGCAAGGCG+TGG	-	chr5.4:49488641-49488660	MS.gene89488:CDS	60.0%
	CTGCGATTCGTCGTCTCGCA+AGG	-	chr5.4:49488636-49488655	MS.gene89488:CDS	60.0%
	CTTGCGAGACGACGAATCGC+AGG	+	chr5.4:49488638-49488657	None:intergenic	60.0%
	GGAAAGGGTCTGGGAAAGGG+TGG	-	chr5.4:49488560-49488579	MS.gene89488:CDS	60.0%
	CGTCGTCTCGCAAGGCGTGG+TGG	-	chr5.4:49488644-49488663	MS.gene89488:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	49488539	49488850	49488539	ID=MS.gene89488
chr5.4	mRNA	49488539	49488850	49488539	ID=MS.gene89488.t1;Parent=MS.gene89488
chr5.4	exon	49488539	49488850	49488539	ID=MS.gene89488.t1.exon1;Parent=MS.gene89488.t1
chr5.4	CDS	49488539	49488850	49488539	ID=cds.MS.gene89488.t1;Parent=MS.gene89488.t1

Gene Sequence	>MS.gene89488 ATGTCAGGAAGAGGCAAAGGAGGAAAGGGTCTGGGAAAGGGTGGTGCAAAGCGTCATCGCAAAGTTCTGAGGGATAACATTCAAGGCATCACCAAACCTGCGATTCGTCGTCTCGCAAGGCGTGGTGGTGTGAAGCGTATCAGTGGTCTGATCTATGAAGAGACTCGCGGTGTTCTCAAGATATTTCTTGAGAATGTTATTCGCGATGCTGTAACTTACACTGAACATGCTCGTCGCAAAACGGTGACTGCTATGGATGTTGTTTATGCACTCAAGAGGCAAGGAAGAACCCTTTATGGTTTTGGGGGTTAG
Protein sequence	>MS.gene89488.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG