AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21005


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21005.t1 MTR_1g084200 97.458 118 3 0 1 118 1 118 4.05e-82 238
MS.gene21005.t1 MTR_7g111900 68.254 126 37 1 1 123 1 126 7.57e-57 174
MS.gene21005.t1 MTR_7g103200 67.460 126 38 1 1 123 1 126 1.70e-56 172
MS.gene21005.t1 MTR_2g019480 49.533 107 54 0 11 117 15 121 1.21e-34 117
MS.gene21005.t1 MTR_2g046150 51.351 111 53 1 3 113 7 116 8.91e-34 115
MS.gene21005.t1 MTR_4g122930 53.636 110 49 1 1 110 1 108 3.33e-33 114
MS.gene21005.t1 MTR_2g091215 44.545 110 61 0 9 118 4 113 7.63e-21 82.0
MS.gene21005.t1 MTR_2g091210 39.048 105 64 0 14 118 9 113 8.97e-21 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21005.t1 AT1G09310 65.323 124 43 0 1 124 1 124 3.04e-56 173
MS.gene21005.t1 AT1G56580 62.992 127 43 1 1 123 1 127 5.21e-55 169
MS.gene21005.t1 AT1G30020 47.959 98 51 0 13 110 15 112 6.73e-31 108
MS.gene21005.t1 AT5G49600 46.512 129 59 2 3 121 1 129 1.82e-30 107
MS.gene21005.t1 AT4G24130 43.103 116 63 1 9 121 13 128 6.81e-30 105
MS.gene21005.t1 AT5G46230 41.818 110 62 1 9 116 11 120 2.17e-29 103

Find 30 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCATTGTATACTTCTGATT+TGG 0.290273 1.3:-59967743 None:intergenic
GTTGATAGGCTTGTGACTTA+TGG 0.360294 1.3:+59967925 MS.gene21005:CDS
TAGAAGAATGTGGATATCAT+AGG 0.386766 1.3:+59967845 MS.gene21005:CDS
ACAAGTTTGAGAAAGTTGAT+AGG 0.420157 1.3:+59967911 MS.gene21005:CDS
GGTAAGATCAAGAAGCTAAC+TGG 0.432806 1.3:+59967976 MS.gene21005:CDS
TATTTCCAAGAGAAGTTCCT+TGG 0.437882 1.3:-59967788 None:intergenic
AGGTGAAGGAGCTCTTGGTT+TGG 0.449978 1.3:+59968004 MS.gene21005:CDS
AAAGTCCAAGGAACTTCTCT+TGG 0.460248 1.3:+59967783 MS.gene21005:CDS
GAAGTTCCTTGGACTTTGCT+TGG 0.463735 1.3:-59967777 None:intergenic
CAGTTGATGGATCATCCAAC+TGG 0.469855 1.3:-59968044 None:intergenic
CAGTTGGATGATCCATCAAC+TGG 0.473384 1.3:+59968045 MS.gene21005:CDS
TTCAAAGGCAAAAGACCATT+GGG 0.479262 1.3:-59967817 None:intergenic
TGATTTGGCTCTGATTTCAT+CGG 0.482547 1.3:-59967728 None:intergenic
TGTGAAGGTGAAGGAGCTCT+TGG 0.487405 1.3:+59967999 MS.gene21005:CDS
CTTCAAAGGCAAAAGACCAT+TGG 0.510255 1.3:-59967818 None:intergenic
CATTCTTCTATGTCCTTCAA+AGG 0.523213 1.3:-59967832 None:intergenic
TGTGGATATCATAGGGAGAG+TGG 0.541120 1.3:+59967853 MS.gene21005:CDS
TCCACTTCATGAAATCCAGT+TGG 0.548501 1.3:+59968029 MS.gene21005:CDS
AGAAGAATGTGGATATCATA+GGG 0.557026 1.3:+59967846 MS.gene21005:CDS
TCCAACTGGATTTCATGAAG+TGG 0.564020 1.3:-59968030 None:intergenic
CAAGAAGCTAACTGGTGTGA+AGG 0.582814 1.3:+59967984 MS.gene21005:CDS
GCTAACTGGTGTGAAGGTGA+AGG 0.589102 1.3:+59967990 MS.gene21005:CDS
AAGGCAAAAGACCATTGGGT+AGG 0.596630 1.3:-59967813 None:intergenic
GCCAAATCAGAAGTATACAA+TGG 0.606422 1.3:+59967742 MS.gene21005:CDS
TGAAGTTACTGCCACTGTTG+AGG 0.621958 1.3:+59967951 MS.gene21005:CDS
TTGAAGGACATAGAAGAATG+TGG 0.627574 1.3:+59967835 MS.gene21005:CDS
GTTACTGCCACTGTTGAGGT+TGG 0.637022 1.3:+59967955 MS.gene21005:CDS
TTGGAAATAAACCTACCCAA+TGG 0.644432 1.3:+59967802 MS.gene21005:CDS
AATCTGCCAAGCAAAGTCCA+AGG 0.673023 1.3:+59967771 MS.gene21005:CDS
GATCTTACCAACCTCAACAG+TGG 0.683396 1.3:-59967962 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGATATAATTAATTTCATTT+GGG - chr1.3:59968530-59968549 None:intergenic 10.0%
!! ATTATAATTTCATATATTCT+TGG - chr1.3:59968411-59968430 None:intergenic 10.0%
!! GAGATATAATTAATTTCATT+TGG - chr1.3:59968531-59968550 None:intergenic 15.0%
!! TAATTCTACAAAAATAAACA+AGG + chr1.3:59968577-59968596 MS.gene21005:intron 15.0%
! ACATTCATTAGAAGAATGAA+AGG + chr1.3:59968619-59968638 MS.gene21005:intron 25.0%
! TCATTCTTCTAATGAATGTA+AGG - chr1.3:59968618-59968637 None:intergenic 25.0%
! TTTCATATACTTTCAGATTC+TGG - chr1.3:59968679-59968698 None:intergenic 25.0%
!! AACATAACATAAGTAGCTTT+TGG - chr1.3:59968283-59968302 None:intergenic 25.0%
!! GATGAGTTAAATAGCATTTT+AGG - chr1.3:59968761-59968780 None:intergenic 25.0%
!!! GTGCTTTTATTTATTTCGTT+AGG - chr1.3:59968553-59968572 None:intergenic 25.0%
!!! TGGTAATGGTAAATTATAAC+TGG - chr1.3:59968489-59968508 None:intergenic 25.0%
!!! TTAAATAGCATTTTAGGTTC+CGG - chr1.3:59968755-59968774 None:intergenic 25.0%
AACTGGTAAAATCACATTCA+GGG + chr1.3:59968062-59968081 MS.gene21005:intron 30.0%
AGAAGAATGTGGATATCATA+GGG + chr1.3:59967846-59967865 MS.gene21005:CDS 30.0%
TAGAAGAATGTGGATATCAT+AGG + chr1.3:59967845-59967864 MS.gene21005:CDS 30.0%
TCCATTGTATACTTCTGATT+TGG - chr1.3:59967746-59967765 None:intergenic 30.0%
! AATGTGATTTTACCAGTTGA+TGG - chr1.3:59968060-59968079 None:intergenic 30.0%
! ACAAGTTTGAGAAAGTTGAT+AGG + chr1.3:59967911-59967930 MS.gene21005:CDS 30.0%
! ATAACAAACAAGCATTGTTG+TGG - chr1.3:59968338-59968357 None:intergenic 30.0%
!! ATGCTTGTTTGTTATGTTTC+AGG + chr1.3:59968343-59968362 MS.gene21005:intron 30.0%
!! TGCTTGTTTGTTATGTTTCA+GGG + chr1.3:59968344-59968363 MS.gene21005:intron 30.0%
CAACTGGTAAAATCACATTC+AGG + chr1.3:59968061-59968080 MS.gene21005:intron 35.0%
CATTCTTCTATGTCCTTCAA+AGG - chr1.3:59967835-59967854 None:intergenic 35.0%
CCATTCCAAGATACTTAATC+CGG + chr1.3:59968733-59968752 MS.gene21005:intron 35.0%
CTTTGAACTTGTAGTTCTCA+TGG - chr1.3:59968835-59968854 None:intergenic 35.0%
CTTTGATTATGCTGCTCTTA+TGG + chr1.3:59968368-59968387 MS.gene21005:intron 35.0%
TATTTCCAAGAGAAGTTCCT+TGG - chr1.3:59967791-59967810 None:intergenic 35.0%
TGAGATAGAAGGAGAAGTTA+AGG + chr1.3:59968125-59968144 MS.gene21005:intron 35.0%
TGAGTATCATAGCATGGTAA+TGG - chr1.3:59968503-59968522 None:intergenic 35.0%
TTCAAAGGCAAAAGACCATT+GGG - chr1.3:59967820-59967839 None:intergenic 35.0%
TTGAAGGACATAGAAGAATG+TGG + chr1.3:59967835-59967854 MS.gene21005:CDS 35.0%
TTGGAAATAAACCTACCCAA+TGG + chr1.3:59967802-59967821 MS.gene21005:CDS 35.0%
! CGCAAACTTTCTGTATGTTT+TGG - chr1.3:59968879-59968898 None:intergenic 35.0%
! GCCAAATCAGAAGTATACAA+TGG + chr1.3:59967742-59967761 MS.gene21005:CDS 35.0%
! GTATCTTGGAATGGATTTGA+AGG - chr1.3:59968727-59968746 None:intergenic 35.0%
!! ATGAAATGCAAGTTCTGAGA+AGG + chr1.3:59968693-59968712 MS.gene21005:intron 35.0%
!! TGATTTGGCTCTGATTTCAT+CGG - chr1.3:59967731-59967750 None:intergenic 35.0%
AAAGTCCAAGGAACTTCTCT+TGG + chr1.3:59967783-59967802 MS.gene21005:CDS 40.0%
AGGAGAAGTTAAGGATGTGA+AGG + chr1.3:59968134-59968153 MS.gene21005:intron 40.0%
AGGTTCCGGATTAAGTATCT+TGG - chr1.3:59968741-59968760 None:intergenic 40.0%
ATCACATTCAGGGCTATTAC+AGG + chr1.3:59968072-59968091 MS.gene21005:intron 40.0%
CCGGATTAAGTATCTTGGAA+TGG - chr1.3:59968736-59968755 None:intergenic 40.0%
CTTCAAAGGCAAAAGACCAT+TGG - chr1.3:59967821-59967840 None:intergenic 40.0%
GAAAGCAGCAAGAGATTGAA+GGG - chr1.3:59968912-59968931 None:intergenic 40.0%
GGATCATGAGTATCATAGCA+TGG - chr1.3:59968509-59968528 None:intergenic 40.0%
GGTAAGATCAAGAAGCTAAC+TGG + chr1.3:59967976-59967995 MS.gene21005:CDS 40.0%
TCCAACTGGATTTCATGAAG+TGG - chr1.3:59968033-59968052 None:intergenic 40.0%
TCCACTTCATGAAATCCAGT+TGG + chr1.3:59968029-59968048 MS.gene21005:CDS 40.0%
TCCTTCTATCTCAAAAGCTG+AGG - chr1.3:59968118-59968137 None:intergenic 40.0%
TGAAAGCAGCAAGAGATTGA+AGG - chr1.3:59968913-59968932 None:intergenic 40.0%
! CAATGGTCTTTTGCCTTTGA+AGG + chr1.3:59967819-59967838 MS.gene21005:CDS 40.0%
! GTTGATAGGCTTGTGACTTA+TGG + chr1.3:59967925-59967944 MS.gene21005:CDS 40.0%
! TCTTGGAATGGATTTGAAGG+TGG - chr1.3:59968724-59968743 None:intergenic 40.0%
!! AATGCAAGTTCTGAGAAGGT+AGG + chr1.3:59968697-59968716 MS.gene21005:intron 40.0%
!!! ATAGGGAGAGTGGTTTTGTA+TGG + chr1.3:59967863-59967882 MS.gene21005:CDS 40.0%
AAGGCAAAAGACCATTGGGT+AGG - chr1.3:59967816-59967835 None:intergenic 45.0%
AATCTGCCAAGCAAAGTCCA+AGG + chr1.3:59967771-59967790 MS.gene21005:CDS 45.0%
CAAGAAGCTAACTGGTGTGA+AGG + chr1.3:59967984-59968003 MS.gene21005:CDS 45.0%
CAGTTGGATGATCCATCAAC+TGG + chr1.3:59968045-59968064 MS.gene21005:CDS 45.0%
CATTGTTGTGGAGCGAGAAA+CGG - chr1.3:59968326-59968345 None:intergenic 45.0%
CTATCTCAAAAGCTGAGGCT+GGG - chr1.3:59968113-59968132 None:intergenic 45.0%
GAAGTTCCTTGGACTTTGCT+TGG - chr1.3:59967780-59967799 None:intergenic 45.0%
GATCTTACCAACCTCAACAG+TGG - chr1.3:59967965-59967984 None:intergenic 45.0%
TCTATCTCAAAAGCTGAGGC+TGG - chr1.3:59968114-59968133 None:intergenic 45.0%
TGAAGTTACTGCCACTGTTG+AGG + chr1.3:59967951-59967970 MS.gene21005:CDS 45.0%
TGCTGCTGTTGAAGTCAAAG+AGG + chr1.3:59968206-59968225 MS.gene21005:intron 45.0%
TGTGGATATCATAGGGAGAG+TGG + chr1.3:59967853-59967872 MS.gene21005:CDS 45.0%
!! CAGTTGATGGATCATCCAAC+TGG - chr1.3:59968047-59968066 None:intergenic 45.0%
!!! GCCTCAGCTTTTGAGATAGA+AGG + chr1.3:59968114-59968133 MS.gene21005:intron 45.0%
AGGTGAAGGAGCTCTTGGTT+TGG + chr1.3:59968004-59968023 MS.gene21005:CDS 50.0%
GCTAACTGGTGTGAAGGTGA+AGG + chr1.3:59967990-59968009 MS.gene21005:CDS 50.0%
GTTACTGCCACTGTTGAGGT+TGG + chr1.3:59967955-59967974 MS.gene21005:CDS 50.0%
TGTGAAGGTGAAGGAGCTCT+TGG + chr1.3:59967999-59968018 MS.gene21005:CDS 50.0%
TTGTGGAGCGAGAAACGGAA+AGG - chr1.3:59968321-59968340 None:intergenic 50.0%
ACAGCAGCAGCAGTAGCAGC+AGG - chr1.3:59968195-59968214 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 59967715 59968965 59967715 ID=MS.gene21005
chr1.3 mRNA 59967715 59968965 59967715 ID=MS.gene21005.t1;Parent=MS.gene21005
chr1.3 exon 59967715 59968066 59967715 ID=MS.gene21005.t1.exon1;Parent=MS.gene21005.t1
chr1.3 CDS 59967715 59968066 59967715 ID=cds.MS.gene21005.t1;Parent=MS.gene21005.t1
chr1.3 exon 59968934 59968965 59968934 ID=MS.gene21005.t1.exon2;Parent=MS.gene21005.t1
chr1.3 CDS 59968934 59968965 59968934 ID=cds.MS.gene21005.t1;Parent=MS.gene21005.t1
Gene Sequence

>MS.gene21005

ATGAATCTTCTTACCGATGAAATCAGAGCCAAATCAGAAGTATACAATGGAGATGAAATCTGCCAAGCAAAGTCCAAGGAACTTCTCTTGGAAATAAACCTACCCAATGGTCTTTTGCCTTTGAAGGACATAGAAGAATGTGGATATCATAGGGAGAGTGGTTTTGTATGGCTTAAGCAGAAAGCAAGCTATACTCACAAGTTTGAGAAAGTTGATAGGCTTGTGACTTATGGTACTGAAGTTACTGCCACTGTTGAGGTTGGTAAGATCAAGAAGCTAACTGGTGTGAAGGTGAAGGAGCTCTTGGTTTGGCTTCCACTTCATGAAATCCAGTTGGATGATCCATCAACTGGTAAAATCACATTCAGGGCTATTACAGGACTTTTCAGAACATTCCCAGCCTCAGCTTTTGAGATAGAAGGAGAAGTTAAGGATGTGAAGGAAGAGAACAAAGATCAAGTCAAAGAAGCAGAAGCACCTGCTGCTACTGCTGCTGCTGTTGAAGTCAAAGAGGTCTAAATCTGATTCTGATTCTGAATTATGCTTATTGATTAAATAAGTTTATCCAAAAGCTACTTATGTTATGTTTCAGTACATTGTAATCCTTTCCGTTTCTCGCTCCACAACAATGCTTGTTTGTTATGTTTCAGGGTCTTTGATTATGCTGCTCTTATGGAGCAGTTTACTTGAAGACCAAGAATATATGAAATTATAATTATTTATCATGATGTATGATCAAAGATATTATTAAAGTTTCTCTTCAAAGATAATCCAGTTATAATTTACCATTACCATGCTATGATACTCATGATCCCAAATGAAATTAATTATATCTCCTAACGAAATAAATAAAAGCACACATTAATTCTACAAAAATAAACAAGGACACATTCTTTAAAACCTTACATTCATTAGAAGAATGAAAGGAATAATATGAAGTCACAAAATAAAACACAACAAACCAGAATCTGAAAGTATATGAAATGCAAGTTCTGAGAAGGTAGGTCCACCTTCAAATCCATTCCAAGATACTTAATCCGGAACCTAAAATGCTATTTAACTCATCAAGTTGAGTTGAAGTTTTAATACATGAAAACTACAAGTACAAAGCTTTGATCCATGAGAACTACAAGTTCAAAGTTGACTAGTAAAGAACTGAGTCCAAAACATACAGAAAGTTTGCGCATGTTTTTTCCCTTCAATCTCTTGCTGCTTTCAGCATTCAAAACTTCAACGCCTGAAATAGTTTAA

Protein sequence

>MS.gene21005.t1

MNLLTDEIRAKSEVYNGDEICQAKSKELLLEINLPNGLLPLKDIEECGYHRESGFVWLKQKASYTHKFEKVDRLVTYGTEVTATVEVGKIKKLTGVKVKELLVWLPLHEIQLDDPSTAFKTSTPEIV