Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22752.t1 | XP_003626612.1 | 81.2 | 186 | 28 | 3 | 1 | 185 | 1 | 180 | 9.20E-73 | 283.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22752.t1 | Q8LCA1 | 56.8 | 183 | 62 | 4 | 5 | 182 | 2 | 172 | 4.0e-48 | 192.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22752.t1 | Q2HTB6 | 81.2 | 186 | 28 | 3 | 1 | 185 | 1 | 180 | 6.7e-73 | 283.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene22752 | 0.802751 | 4.83E-49 | -1.69E-46 |
| MS.gene049171 | MS.gene22752 | 0.853425 | 2.41E-61 | -1.69E-46 |
| MS.gene049222 | MS.gene22752 | 0.840658 | 7.61E-58 | -1.69E-46 |
| MS.gene049232 | MS.gene22752 | 0.802828 | 4.66E-49 | -1.69E-46 |
| MS.gene049285 | MS.gene22752 | 0.85566 | 5.44E-62 | -1.69E-46 |
| MS.gene049320 | MS.gene22752 | 0.863625 | 2.18E-64 | -1.69E-46 |
| MS.gene049609 | MS.gene22752 | 0.876632 | 1.20E-68 | -1.69E-46 |
| MS.gene049615 | MS.gene22752 | 0.844988 | 5.37E-59 | -1.69E-46 |
| MS.gene049633 | MS.gene22752 | 0.807503 | 4.90E-50 | -1.69E-46 |
| MS.gene049805 | MS.gene22752 | 0.825709 | 4.04E-54 | -1.69E-46 |
| MS.gene05010 | MS.gene22752 | 0.852352 | 4.88E-61 | -1.69E-46 |
| MS.gene050163 | MS.gene22752 | 0.807982 | 3.87E-50 | -1.69E-46 |
| MS.gene050170 | MS.gene22752 | 0.801786 | 7.64E-49 | -1.69E-46 |
| MS.gene050504 | MS.gene22752 | 0.835067 | 2.08E-56 | -1.69E-46 |
| MS.gene050518 | MS.gene22752 | 0.85507 | 8.07E-62 | -1.69E-46 |
| MS.gene050538 | MS.gene22752 | 0.81247 | 4.17E-51 | -1.69E-46 |
| MS.gene050577 | MS.gene22752 | 0.833459 | 5.27E-56 | -1.69E-46 |
| MS.gene050593 | MS.gene22752 | 0.891753 | 2.98E-74 | -1.69E-46 |
| MS.gene050612 | MS.gene22752 | 0.850133 | 2.07E-60 | -1.69E-46 |
| MS.gene050675 | MS.gene22752 | 0.845167 | 4.81E-59 | -1.69E-46 |
| MS.gene050699 | MS.gene22752 | 0.896109 | 5.06E-76 | -1.69E-46 |
| MS.gene050747 | MS.gene22752 | 0.859121 | 5.15E-63 | -1.69E-46 |
| MS.gene050772 | MS.gene22752 | 0.883128 | 5.83E-71 | -1.69E-46 |
| MS.gene05080 | MS.gene22752 | 0.827764 | 1.31E-54 | -1.69E-46 |
| MS.gene050950 | MS.gene22752 | 0.861446 | 1.02E-63 | -1.69E-46 |
| MS.gene050986 | MS.gene22752 | 0.858859 | 6.17E-63 | -1.69E-46 |
| MS.gene05105 | MS.gene22752 | 0.801969 | 7.01E-49 | -1.69E-46 |
| MS.gene051317 | MS.gene22752 | 0.80542 | 1.35E-49 | -1.69E-46 |
| MS.gene051387 | MS.gene22752 | 0.857701 | 1.36E-62 | -1.69E-46 |
| MS.gene051551 | MS.gene22752 | 0.845357 | 4.27E-59 | -1.69E-46 |
| MS.gene051666 | MS.gene22752 | 0.816013 | 6.88E-52 | -1.69E-46 |
| MS.gene051838 | MS.gene22752 | 0.802062 | 6.70E-49 | -1.69E-46 |
| MS.gene052271 | MS.gene22752 | 0.810217 | 1.29E-50 | -1.69E-46 |
| MS.gene052501 | MS.gene22752 | 0.807241 | 5.57E-50 | -1.69E-46 |
| MS.gene052503 | MS.gene22752 | 0.824291 | 8.74E-54 | -1.69E-46 |
| MS.gene052504 | MS.gene22752 | 0.831336 | 1.77E-55 | -1.69E-46 |
| MS.gene052505 | MS.gene22752 | 0.80343 | 3.50E-49 | -1.69E-46 |
| MS.gene052506 | MS.gene22752 | 0.851756 | 7.21E-61 | -1.69E-46 |
| MS.gene052510 | MS.gene22752 | 0.801004 | 1.10E-48 | -1.69E-46 |
| MS.gene052615 | MS.gene22752 | 0.841803 | 3.81E-58 | -1.69E-46 |
| MS.gene052820 | MS.gene22752 | 0.81725 | 3.64E-52 | -1.69E-46 |
| MS.gene052822 | MS.gene22752 | 0.843412 | 1.42E-58 | -1.69E-46 |
| MS.gene052823 | MS.gene22752 | 0.817991 | 2.47E-52 | -1.69E-46 |
| MS.gene052825 | MS.gene22752 | 0.815119 | 1.09E-51 | -1.69E-46 |
| MS.gene052966 | MS.gene22752 | 0.804032 | 2.62E-49 | -1.69E-46 |
| MS.gene053104 | MS.gene22752 | 0.800063 | 1.72E-48 | -1.69E-46 |
| MS.gene053107 | MS.gene22752 | 0.82624 | 3.02E-54 | -1.69E-46 |
| MS.gene053203 | MS.gene22752 | 0.882258 | 1.21E-70 | -1.69E-46 |
| MS.gene053207 | MS.gene22752 | 0.874476 | 6.56E-68 | -1.69E-46 |
| MS.gene053213 | MS.gene22752 | 0.888422 | 5.99E-73 | -1.69E-46 |
| MS.gene053286 | MS.gene22752 | 0.884098 | 2.56E-71 | -1.69E-46 |
| MS.gene053436 | MS.gene22752 | 0.87081 | 1.10E-66 | -1.69E-46 |
| MS.gene053479 | MS.gene22752 | 0.824058 | 9.92E-54 | -1.69E-46 |
| MS.gene053574 | MS.gene22752 | 0.873251 | 1.70E-67 | -1.69E-46 |
| MS.gene053579 | MS.gene22752 | 0.814088 | 1.84E-51 | -1.69E-46 |
| MS.gene053626 | MS.gene22752 | 0.811148 | 8.09E-51 | -1.69E-46 |
| MS.gene053632 | MS.gene22752 | 0.808424 | 3.12E-50 | -1.69E-46 |
| MS.gene053824 | MS.gene22752 | 0.803388 | 3.57E-49 | -1.69E-46 |
| MS.gene054408 | MS.gene22752 | 0.803669 | 3.12E-49 | -1.69E-46 |
| MS.gene054595 | MS.gene22752 | 0.844496 | 7.29E-59 | -1.69E-46 |
| MS.gene054600 | MS.gene22752 | 0.854946 | 8.77E-62 | -1.69E-46 |
| MS.gene054642 | MS.gene22752 | 0.860545 | 1.92E-63 | -1.69E-46 |
| MS.gene054927 | MS.gene22752 | 0.843113 | 1.71E-58 | -1.69E-46 |
| MS.gene054954 | MS.gene22752 | 0.839345 | 1.67E-57 | -1.69E-46 |
| MS.gene055206 | MS.gene22752 | 0.833474 | 5.22E-56 | -1.69E-46 |
| MS.gene055224 | MS.gene22752 | 0.813274 | 2.78E-51 | -1.69E-46 |
| MS.gene055289 | MS.gene22752 | 0.81911 | 1.38E-52 | -1.69E-46 |
| MS.gene055362 | MS.gene22752 | 0.865801 | 4.54E-65 | -1.69E-46 |
| MS.gene055502 | MS.gene22752 | 0.840201 | 1.00E-57 | -1.69E-46 |
| MS.gene055577 | MS.gene22752 | 0.845876 | 3.09E-59 | -1.69E-46 |
| MS.gene055647 | MS.gene22752 | 0.85541 | 6.43E-62 | -1.69E-46 |
| MS.gene055722 | MS.gene22752 | 0.849959 | 2.31E-60 | -1.69E-46 |
| MS.gene05592 | MS.gene22752 | 0.844221 | 8.65E-59 | -1.69E-46 |
| MS.gene055941 | MS.gene22752 | 0.814919 | 1.21E-51 | -1.69E-46 |
| MS.gene056040 | MS.gene22752 | 0.831006 | 2.13E-55 | -1.69E-46 |
| MS.gene056083 | MS.gene22752 | 0.828828 | 7.24E-55 | -1.69E-46 |
| MS.gene056374 | MS.gene22752 | 0.856782 | 2.55E-62 | -1.69E-46 |
| MS.gene056504 | MS.gene22752 | 0.807405 | 5.14E-50 | -1.69E-46 |
| MS.gene056510 | MS.gene22752 | 0.837459 | 5.13E-57 | -1.69E-46 |
| MS.gene056610 | MS.gene22752 | 0.801703 | 7.94E-49 | -1.69E-46 |
| MS.gene056657 | MS.gene22752 | 0.845321 | 4.37E-59 | -1.69E-46 |
| MS.gene056692 | MS.gene22752 | 0.802785 | 4.76E-49 | -1.69E-46 |
| MS.gene056877 | MS.gene22752 | 0.811562 | 6.58E-51 | -1.69E-46 |
| MS.gene057011 | MS.gene22752 | 0.825084 | 5.69E-54 | -1.69E-46 |
| MS.gene057076 | MS.gene22752 | 0.801619 | 8.27E-49 | -1.69E-46 |
| MS.gene057129 | MS.gene22752 | 0.808367 | 3.21E-50 | -1.69E-46 |
| MS.gene057196 | MS.gene22752 | 0.812857 | 3.43E-51 | -1.69E-46 |
| MS.gene057331 | MS.gene22752 | 0.909674 | 4.39E-82 | -1.69E-46 |
| MS.gene057332 | MS.gene22752 | 0.821534 | 3.84E-53 | -1.69E-46 |
| MS.gene057416 | MS.gene22752 | 0.89513 | 1.28E-75 | -1.69E-46 |
| MS.gene057658 | MS.gene22752 | 0.804974 | 1.67E-49 | -1.69E-46 |
| MS.gene057831 | MS.gene22752 | 0.823913 | 1.07E-53 | -1.69E-46 |
| MS.gene058019 | MS.gene22752 | 0.83853 | 2.72E-57 | -1.69E-46 |
| MS.gene058323 | MS.gene22752 | 0.830239 | 3.28E-55 | -1.69E-46 |
| MS.gene05837 | MS.gene22752 | 0.860866 | 1.53E-63 | -1.69E-46 |
| MS.gene058419 | MS.gene22752 | 0.86817 | 7.96E-66 | -1.69E-46 |
| MS.gene058544 | MS.gene22752 | 0.874313 | 7.45E-68 | -1.69E-46 |
| MS.gene058773 | MS.gene22752 | 0.841541 | 4.46E-58 | -1.69E-46 |
| MS.gene058838 | MS.gene22752 | 0.818079 | 2.36E-52 | -1.69E-46 |
| MS.gene05883 | MS.gene22752 | 0.841014 | 6.14E-58 | -1.69E-46 |
| MS.gene059022 | MS.gene22752 | 0.838891 | 2.20E-57 | -1.69E-46 |
| MS.gene059023 | MS.gene22752 | 0.847605 | 1.04E-59 | -1.69E-46 |
| MS.gene05934 | MS.gene22752 | 0.802002 | 6.90E-49 | -1.69E-46 |
| MS.gene059677 | MS.gene22752 | 0.842721 | 2.17E-58 | -1.69E-46 |
| MS.gene059718 | MS.gene22752 | 0.853319 | 2.58E-61 | -1.69E-46 |
| MS.gene059756 | MS.gene22752 | 0.851336 | 9.49E-61 | -1.69E-46 |
| MS.gene059865 | MS.gene22752 | 0.817747 | 2.81E-52 | -1.69E-46 |
| MS.gene059960 | MS.gene22752 | 0.80496 | 1.68E-49 | -1.69E-46 |
| MS.gene060193 | MS.gene22752 | 0.800552 | 1.37E-48 | -1.69E-46 |
| MS.gene060403 | MS.gene22752 | 0.813862 | 2.06E-51 | -1.69E-46 |
| MS.gene060482 | MS.gene22752 | 0.813935 | 1.99E-51 | -1.69E-46 |
| MS.gene06077 | MS.gene22752 | 0.842459 | 2.55E-58 | -1.69E-46 |
| MS.gene060804 | MS.gene22752 | 0.802555 | 5.31E-49 | -1.69E-46 |
| MS.gene060835 | MS.gene22752 | 0.836761 | 7.74E-57 | -1.69E-46 |
| MS.gene06092 | MS.gene22752 | 0.892867 | 1.07E-74 | -1.69E-46 |
| MS.gene060982 | MS.gene22752 | 0.820923 | 5.31E-53 | -1.69E-46 |
| MS.gene061150 | MS.gene22752 | 0.860209 | 2.42E-63 | -1.69E-46 |
| MS.gene061200 | MS.gene22752 | 0.808021 | 3.80E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene38715 | MS.gene22752 | PPI |
| MS.gene012982 | MS.gene22752 | PPI |
| MS.gene98588 | MS.gene22752 | PPI |
| MS.gene48506 | MS.gene22752 | PPI |
| MS.gene044778 | MS.gene22752 | PPI |
| MS.gene040618 | MS.gene22752 | PPI |
| MS.gene88194 | MS.gene22752 | PPI |
| MS.gene66718 | MS.gene22752 | PPI |
| MS.gene039151 | MS.gene22752 | PPI |
| MS.gene054642 | MS.gene22752 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22752.t1 | MTR_7g118290 | 90.698 | 172 | 9 | 3 | 15 | 185 | 15 | 180 | 5.15e-109 | 309 |
| MS.gene22752.t1 | MTR_7g118290 | 78.488 | 172 | 9 | 4 | 15 | 185 | 15 | 159 | 3.09e-87 | 253 |
| MS.gene22752.t1 | MTR_7g118290 | 79.710 | 138 | 16 | 5 | 15 | 151 | 15 | 141 | 2.38e-67 | 203 |
| MS.gene22752.t1 | MTR_8g024180 | 56.962 | 158 | 52 | 3 | 25 | 182 | 27 | 168 | 9.60e-50 | 159 |
| MS.gene22752.t1 | MTR_4g013200 | 35.172 | 145 | 86 | 1 | 38 | 182 | 9 | 145 | 3.55e-25 | 95.5 |
| MS.gene22752.t1 | MTR_7g116795 | 31.410 | 156 | 89 | 3 | 29 | 184 | 24 | 161 | 4.73e-21 | 85.1 |
| MS.gene22752.t1 | MTR_5g032440 | 28.696 | 115 | 81 | 1 | 69 | 182 | 67 | 181 | 8.36e-15 | 69.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22752.t1 | AT2G46820 | 62.585 | 147 | 46 | 2 | 36 | 182 | 35 | 172 | 1.51e-56 | 176 |
| MS.gene22752.t1 | AT2G46820 | 62.585 | 147 | 46 | 2 | 36 | 182 | 35 | 172 | 1.51e-56 | 176 |
| MS.gene22752.t1 | AT1G52220 | 47.525 | 101 | 52 | 1 | 82 | 182 | 56 | 155 | 8.49e-27 | 99.8 |
| MS.gene22752.t1 | AT1G52220 | 47.525 | 101 | 51 | 2 | 82 | 182 | 56 | 154 | 4.87e-25 | 95.5 |
| MS.gene22752.t1 | AT4G01150 | 32.919 | 161 | 87 | 4 | 25 | 182 | 20 | 162 | 5.29e-23 | 90.5 |
| MS.gene22752.t1 | AT4G38100 | 35.789 | 95 | 58 | 1 | 91 | 185 | 101 | 192 | 1.08e-14 | 69.3 |
| MS.gene22752.t1 | AT4G38100 | 35.789 | 95 | 58 | 1 | 91 | 185 | 61 | 152 | 1.78e-14 | 67.8 |
| MS.gene22752.t1 | AT1G52220 | 46.479 | 71 | 37 | 1 | 82 | 152 | 56 | 125 | 1.22e-13 | 65.5 |
| MS.gene22752.t1 | AT1G52220 | 52.000 | 50 | 24 | 0 | 133 | 182 | 77 | 126 | 2.00e-12 | 61.6 |
Find 56 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTATAGTCTTTAGAAATTC+TGG | 0.176189 | 7.3:+360743 | None:intergenic |
| GATAGGCTTCCTTTAATTCC+TGG | 0.266011 | 7.3:-360453 | MS.gene22752:CDS |
| TCTTCATTCACTCTTATTGA+TGG | 0.283095 | 7.3:-361074 | MS.gene22752:CDS |
| ACAGAAAGCGGTGGTCTTCC+AGG | 0.290167 | 7.3:+360965 | None:intergenic |
| CTCTGTTTCTGTTGTGGTAC+TGG | 0.316852 | 7.3:+360826 | None:intergenic |
| GTTGCATTATGGGGCTCAGC+TGG | 0.318460 | 7.3:-360572 | MS.gene22752:CDS |
| TCAAGAACCCCAGGAATTAA+AGG | 0.320013 | 7.3:+360444 | None:intergenic |
| TAGCAGGTACGGTTGCATTA+TGG | 0.322048 | 7.3:-360583 | MS.gene22752:CDS |
| AGCAGGTACGGTTGCATTAT+GGG | 0.357874 | 7.3:-360582 | MS.gene22752:CDS |
| ATAGGCTTCCTTTAATTCCT+GGG | 0.369347 | 7.3:-360452 | MS.gene22752:CDS |
| AAGAGTGAATGAAGAGAAAG+TGG | 0.378344 | 7.3:+361082 | None:intergenic |
| TGGTCTTCCAGGGACGAGTA+TGG | 0.381671 | 7.3:+360976 | None:intergenic |
| GCTTTGATACGCAAAATAAA+AGG | 0.394589 | 7.3:-360181 | MS.gene22752:CDS |
| TTGCATTATGGGGCTCAGCT+GGG | 0.412672 | 7.3:-360571 | MS.gene22752:CDS |
| GAAAGTGGAAGATGAGGCTG+AGG | 0.422883 | 7.3:+361097 | None:intergenic |
| CTCGTAGGCATTGGCTACAC+TGG | 0.433498 | 7.3:-360420 | MS.gene22752:CDS |
| TGATGAAACAGGCTATTGAT+AGG | 0.459015 | 7.3:-360470 | MS.gene22752:intron |
| ACCGATCTGGCTTGAAAACT+AGG | 0.468356 | 7.3:+360282 | None:intergenic |
| TGGAGAAGCAGAGTGTCTGC+AGG | 0.469794 | 7.3:+361043 | None:intergenic |
| TGCTCTAGCTGTAGCAGGTA+CGG | 0.487789 | 7.3:-360594 | MS.gene22752:CDS |
| TGCATTATGGGGCTCAGCTG+GGG | 0.492851 | 7.3:-360570 | MS.gene22752:CDS |
| TGGGACTGAACAGGTGGAGG+GGG | 0.502807 | 7.3:+360996 | None:intergenic |
| ATCTGCCTCTGTTTCTGTTG+TGG | 0.505718 | 7.3:+360820 | None:intergenic |
| TTCAGTCCCATACTCGTCCC+TGG | 0.506421 | 7.3:-360983 | MS.gene22752:CDS |
| GTTCTTGAACTCGTAGGCAT+TGG | 0.511626 | 7.3:-360429 | MS.gene22752:CDS |
| GGACGAGTATGGGACTGAAC+AGG | 0.517757 | 7.3:+360987 | None:intergenic |
| TGCTCGCAATGTTATGTCCA+TGG | 0.518500 | 7.3:-360852 | MS.gene22752:CDS |
| AGTGGAAGATGAGGCTGAGG+AGG | 0.528576 | 7.3:+361100 | None:intergenic |
| GTGAGTGCTCTAGCTGTAGC+AGG | 0.528606 | 7.3:-360599 | MS.gene22752:CDS |
| GGCACTGTTAAAGACATCAC+TGG | 0.529095 | 7.3:-360160 | MS.gene22752:CDS |
| GGTCTTCCAGGGACGAGTAT+GGG | 0.539747 | 7.3:+360977 | None:intergenic |
| AGAATCAGTGACAGTAGTAC+TGG | 0.542464 | 7.3:+360766 | None:intergenic |
| AAGAGCATAGCATACCGATC+TGG | 0.544761 | 7.3:+360269 | None:intergenic |
| AGCTGAGGCAACCTCTGTTG+TGG | 0.548680 | 7.3:+360793 | None:intergenic |
| GTATGGGACTGAACAGGTGG+AGG | 0.553903 | 7.3:+360993 | None:intergenic |
| TAGGCTTCCTTTAATTCCTG+GGG | 0.554551 | 7.3:-360451 | MS.gene22752:CDS |
| TCGTAGGCATTGGCTACACT+GGG | 0.562769 | 7.3:-360419 | MS.gene22752:CDS |
| TATGGGACTGAACAGGTGGA+GGG | 0.576384 | 7.3:+360994 | None:intergenic |
| CAGAAAGCGGTGGTCTTCCA+GGG | 0.585352 | 7.3:+360966 | None:intergenic |
| TGTATTTGACTTACAGAAAG+CGG | 0.587004 | 7.3:+360953 | None:intergenic |
| TGTTGTGGTACTGGAAGCCA+TGG | 0.596653 | 7.3:+360835 | None:intergenic |
| CGAGTATGGGACTGAACAGG+TGG | 0.598195 | 7.3:+360990 | None:intergenic |
| ATGGGACTGAACAGGTGGAG+GGG | 0.599012 | 7.3:+360995 | None:intergenic |
| GCAGGTACGGTTGCATTATG+GGG | 0.601282 | 7.3:-360581 | MS.gene22752:CDS |
| TGAAGAGAAAGTGGAAGATG+AGG | 0.604075 | 7.3:+361091 | None:intergenic |
| GTTGGAAGGCTCACGCATTG+TGG | 0.611890 | 7.3:+361023 | None:intergenic |
| CCTGGGGTTCTTGAACTCGT+AGG | 0.612235 | 7.3:-360435 | MS.gene22752:CDS |
| CCTACGAGTTCAAGAACCCC+AGG | 0.617642 | 7.3:+360435 | None:intergenic |
| GGAGAAGCAGAGTGTCTGCA+GGG | 0.624844 | 7.3:+361044 | None:intergenic |
| AGTAGTACTGGTGTCAGCTG+AGG | 0.645500 | 7.3:+360778 | None:intergenic |
| GCACTGTTAAAGACATCACT+GGG | 0.652022 | 7.3:-360159 | MS.gene22752:CDS |
| GGCAGATGCAGCCACAACAG+AGG | 0.654208 | 7.3:-360804 | MS.gene22752:CDS |
| CAGTACCACAACAGAAACAG+AGG | 0.682552 | 7.3:-360825 | MS.gene22752:CDS |
| ATTTGACTTACAGAAAGCGG+TGG | 0.698122 | 7.3:+360956 | None:intergenic |
| GAAGCAGAGTGTCTGCAGGG+AGG | 0.716854 | 7.3:+361047 | None:intergenic |
| CGTAGGCATTGGCTACACTG+GGG | 0.765097 | 7.3:-360418 | MS.gene22752:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATATATATTATATGCAAAA+AGG | + | chr7.3:360727-360746 | None:intergenic | 10.0% |
| !! | AATTAATAATATATAATTGC+AGG | - | chr7.3:361044-361063 | MS.gene22752:CDS | 10.0% |
| !! | ATTAATAATATATAATTGCA+GGG | - | chr7.3:361045-361064 | MS.gene22752:CDS | 10.0% |
| !! | AATAATCTGTTTCCATAATT+TGG | - | chr7.3:361009-361028 | MS.gene22752:CDS | 20.0% |
| !! | ATATAATTGAATTTGTGTGT+TGG | - | chr7.3:360353-360372 | MS.gene22752:intron | 20.0% |
| !! | TATATTATATGCAAAAAGGA+AGG | + | chr7.3:360723-360742 | None:intergenic | 20.0% |
| ! | AATAATATATAATTGCAGGG+AGG | - | chr7.3:361048-361067 | MS.gene22752:CDS | 25.0% |
| ! | CAATCTATTGAACCAAATTA+TGG | + | chr7.3:361024-361043 | None:intergenic | 25.0% |
| !! | TGTATAGTCTTTAGAAATTC+TGG | + | chr7.3:360511-360530 | None:intergenic | 25.0% |
| ATAGTATATGTGTGTTGAGT+TGG | - | chr7.3:360584-360603 | MS.gene22752:CDS | 30.0% | |
| GATTGGATGTAATGTATAGT+GGG | - | chr7.3:360606-360625 | MS.gene22752:CDS | 30.0% | |
| GCTTTGATACGCAAAATAAA+AGG | - | chr7.3:361070-361089 | MS.gene22752:CDS | 30.0% | |
| TCTTCATTCACTCTTATTGA+TGG | - | chr7.3:360177-360196 | MS.gene22752:CDS | 30.0% | |
| ! | TGTATTTGACTTACAGAAAG+CGG | + | chr7.3:360301-360320 | None:intergenic | 30.0% |
| AAGAGTGAATGAAGAGAAAG+TGG | + | chr7.3:360172-360191 | None:intergenic | 35.0% | |
| ATAGGCTTCCTTTAATTCCT+GGG | - | chr7.3:360799-360818 | MS.gene22752:CDS | 35.0% | |
| ATCTGCAGAAGATACAAAGT+AGG | + | chr7.3:360865-360884 | None:intergenic | 35.0% | |
| GGATTGGATGTAATGTATAG+TGG | - | chr7.3:360605-360624 | MS.gene22752:CDS | 35.0% | |
| TGATGAAACAGGCTATTGAT+AGG | - | chr7.3:360781-360800 | MS.gene22752:CDS | 35.0% | |
| !! | ATATGTGTGTTGAGTTGGAT+TGG | - | chr7.3:360589-360608 | MS.gene22752:CDS | 35.0% |
| AGAATCAGTGACAGTAGTAC+TGG | + | chr7.3:360488-360507 | None:intergenic | 40.0% | |
| GATAGGCTTCCTTTAATTCC+TGG | - | chr7.3:360798-360817 | MS.gene22752:CDS | 40.0% | |
| GCACTGTTAAAGACATCACT+GGG | - | chr7.3:361092-361111 | MS.gene22752:CDS | 40.0% | |
| TAGGCTTCCTTTAATTCCTG+GGG | - | chr7.3:360800-360819 | MS.gene22752:CDS | 40.0% | |
| TCAAGAACCCCAGGAATTAA+AGG | + | chr7.3:360810-360829 | None:intergenic | 40.0% | |
| TCGTATCTTCTTCTTTGCAG+TGG | - | chr7.3:360932-360951 | MS.gene22752:intron | 40.0% | |
| TGAAGAGAAAGTGGAAGATG+AGG | + | chr7.3:360163-360182 | None:intergenic | 40.0% | |
| ! | ATTTGACTTACAGAAAGCGG+TGG | + | chr7.3:360298-360317 | None:intergenic | 40.0% |
| !! | ACCTAGTTTTCAAGCCAGAT+CGG | - | chr7.3:360968-360987 | MS.gene22752:CDS | 40.0% |
| AGCAGGTACGGTTGCATTAT+GGG | - | chr7.3:360669-360688 | MS.gene22752:intron | 45.0% | |
| CAGTACCACAACAGAAACAG+AGG | - | chr7.3:360426-360445 | MS.gene22752:CDS | 45.0% | |
| CTCACTGCATCTGATGAAAC+AGG | - | chr7.3:360770-360789 | MS.gene22752:CDS | 45.0% | |
| GGCACTGTTAAAGACATCAC+TGG | - | chr7.3:361091-361110 | MS.gene22752:CDS | 45.0% | |
| GTTCTTGAACTCGTAGGCAT+TGG | - | chr7.3:360822-360841 | MS.gene22752:CDS | 45.0% | |
| TAGCAGGTACGGTTGCATTA+TGG | - | chr7.3:360668-360687 | MS.gene22752:intron | 45.0% | |
| TGCTCGCAATGTTATGTCCA+TGG | - | chr7.3:360399-360418 | MS.gene22752:intron | 45.0% | |
| ! | AAGAGCATAGCATACCGATC+TGG | + | chr7.3:360985-361004 | None:intergenic | 45.0% |
| ! | ACCGATCTGGCTTGAAAACT+AGG | + | chr7.3:360972-360991 | None:intergenic | 45.0% |
| ! | GTTGGTTGATTCACTCAGGT+CGG | - | chr7.3:360371-360390 | MS.gene22752:intron | 45.0% |
| !! | ATCTGCCTCTGTTTCTGTTG+TGG | + | chr7.3:360434-360453 | None:intergenic | 45.0% |
| !! | CTCTGTTTCTGTTGTGGTAC+TGG | + | chr7.3:360428-360447 | None:intergenic | 45.0% |
| !! | GTGTGTTGGTTGATTCACTC+AGG | - | chr7.3:360367-360386 | MS.gene22752:intron | 45.0% |
| GAAAGTGGAAGATGAGGCTG+AGG | + | chr7.3:360157-360176 | None:intergenic | 50.0% | |
| GCAGGTACGGTTGCATTATG+GGG | - | chr7.3:360670-360689 | MS.gene22752:intron | 50.0% | |
| TATGGGACTGAACAGGTGGA+GGG | + | chr7.3:360260-360279 | None:intergenic | 50.0% | |
| TGCTCTAGCTGTAGCAGGTA+CGG | - | chr7.3:360657-360676 | MS.gene22752:intron | 50.0% | |
| TTGCATTATGGGGCTCAGCT+GGG | - | chr7.3:360680-360699 | MS.gene22752:intron | 50.0% | |
| ! | TGTTGTGGTACTGGAAGCCA+TGG | + | chr7.3:360419-360438 | None:intergenic | 50.0% |
| !! | AGTAGTACTGGTGTCAGCTG+AGG | + | chr7.3:360476-360495 | None:intergenic | 50.0% |
| !! | TCGTAGGCATTGGCTACACT+GGG | - | chr7.3:360832-360851 | MS.gene22752:CDS | 50.0% |
| ACAGAAAGCGGTGGTCTTCC+AGG | + | chr7.3:360289-360308 | None:intergenic | 55.0% | |
| AGCTGAGGCAACCTCTGTTG+TGG | + | chr7.3:360461-360480 | None:intergenic | 55.0% | |
| AGTGGAAGATGAGGCTGAGG+AGG | + | chr7.3:360154-360173 | None:intergenic | 55.0% | |
| ATGGGACTGAACAGGTGGAG+GGG | + | chr7.3:360259-360278 | None:intergenic | 55.0% | |
| CAGAAAGCGGTGGTCTTCCA+GGG | + | chr7.3:360288-360307 | None:intergenic | 55.0% | |
| CCTACGAGTTCAAGAACCCC+AGG | + | chr7.3:360819-360838 | None:intergenic | 55.0% | |
| CCTGGGGTTCTTGAACTCGT+AGG | - | chr7.3:360816-360835 | MS.gene22752:CDS | 55.0% | |
| CGAGTATGGGACTGAACAGG+TGG | + | chr7.3:360264-360283 | None:intergenic | 55.0% | |
| GGACGAGTATGGGACTGAAC+AGG | + | chr7.3:360267-360286 | None:intergenic | 55.0% | |
| GGTCTTCCAGGGACGAGTAT+GGG | + | chr7.3:360277-360296 | None:intergenic | 55.0% | |
| GTATGGGACTGAACAGGTGG+AGG | + | chr7.3:360261-360280 | None:intergenic | 55.0% | |
| GTGAGTGCTCTAGCTGTAGC+AGG | - | chr7.3:360652-360671 | MS.gene22752:intron | 55.0% | |
| GTTGCATTATGGGGCTCAGC+TGG | - | chr7.3:360679-360698 | MS.gene22752:intron | 55.0% | |
| GTTGGAAGGCTCACGCATTG+TGG | + | chr7.3:360231-360250 | None:intergenic | 55.0% | |
| TGCATTATGGGGCTCAGCTG+GGG | - | chr7.3:360681-360700 | MS.gene22752:intron | 55.0% | |
| TGGTCTTCCAGGGACGAGTA+TGG | + | chr7.3:360278-360297 | None:intergenic | 55.0% | |
| TTCAGTCCCATACTCGTCCC+TGG | - | chr7.3:360268-360287 | MS.gene22752:intron | 55.0% | |
| ! | GGAGAAGCAGAGTGTCTGCA+GGG | + | chr7.3:360210-360229 | None:intergenic | 55.0% |
| ! | TGGAGAAGCAGAGTGTCTGC+AGG | + | chr7.3:360211-360230 | None:intergenic | 55.0% |
| !! | CGTAGGCATTGGCTACACTG+GGG | - | chr7.3:360833-360852 | MS.gene22752:CDS | 55.0% |
| !! | CTCGTAGGCATTGGCTACAC+TGG | - | chr7.3:360831-360850 | MS.gene22752:CDS | 55.0% |
| ACAGGTGGAGGGGGAAGAGT+TGG | + | chr7.3:360249-360268 | None:intergenic | 60.0% | |
| GGCAGATGCAGCCACAACAG+AGG | - | chr7.3:360447-360466 | MS.gene22752:CDS | 60.0% | |
| GTGGAGGGGGAAGAGTTGGA+AGG | + | chr7.3:360245-360264 | None:intergenic | 60.0% | |
| TGGGACTGAACAGGTGGAGG+GGG | + | chr7.3:360258-360277 | None:intergenic | 60.0% | |
| ! | GAAGCAGAGTGTCTGCAGGG+AGG | + | chr7.3:360207-360226 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 360147 | 361126 | 360147 | ID=MS.gene22752 |
| chr7.3 | mRNA | 360147 | 361126 | 360147 | ID=MS.gene22752.t1;Parent=MS.gene22752 |
| chr7.3 | exon | 360967 | 361126 | 360967 | ID=MS.gene22752.t1.exon1;Parent=MS.gene22752.t1 |
| chr7.3 | CDS | 360967 | 361126 | 360967 | ID=cds.MS.gene22752.t1;Parent=MS.gene22752.t1 |
| chr7.3 | exon | 360736 | 360884 | 360736 | ID=MS.gene22752.t1.exon2;Parent=MS.gene22752.t1 |
| chr7.3 | CDS | 360736 | 360884 | 360736 | ID=cds.MS.gene22752.t1;Parent=MS.gene22752.t1 |
| chr7.3 | exon | 360562 | 360648 | 360562 | ID=MS.gene22752.t1.exon3;Parent=MS.gene22752.t1 |
| chr7.3 | CDS | 360562 | 360648 | 360562 | ID=cds.MS.gene22752.t1;Parent=MS.gene22752.t1 |
| chr7.3 | exon | 360419 | 360481 | 360419 | ID=MS.gene22752.t1.exon4;Parent=MS.gene22752.t1 |
| chr7.3 | CDS | 360419 | 360481 | 360419 | ID=cds.MS.gene22752.t1;Parent=MS.gene22752.t1 |
| chr7.3 | exon | 360284 | 360321 | 360284 | ID=MS.gene22752.t1.exon5;Parent=MS.gene22752.t1 |
| chr7.3 | CDS | 360284 | 360321 | 360284 | ID=cds.MS.gene22752.t1;Parent=MS.gene22752.t1 |
| chr7.3 | exon | 360147 | 360207 | 360147 | ID=MS.gene22752.t1.exon6;Parent=MS.gene22752.t1 |
| chr7.3 | CDS | 360147 | 360207 | 360147 | ID=cds.MS.gene22752.t1;Parent=MS.gene22752.t1 |
| Gene Sequence |
| Protein sequence |
