Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68873.t1 | XP_003610489.2 | 98.9 | 187 | 2 | 0 | 1 | 187 | 582 | 768 | 1.10E-84 | 322.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68873.t1 | O48802 | 71.1 | 173 | 43 | 2 | 1 | 171 | 584 | 751 | 6.6e-51 | 201.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68873.t1 | G7JKF9 | 98.9 | 187 | 2 | 0 | 1 | 187 | 582 | 768 | 7.7e-85 | 322.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene68873 | 0.804841 | 1.78E-49 | -1.69E-46 |
| MS.gene049230 | MS.gene68873 | 0.832034 | 1.19E-55 | -1.69E-46 |
| MS.gene049297 | MS.gene68873 | 0.834034 | 3.79E-56 | -1.69E-46 |
| MS.gene049378 | MS.gene68873 | 0.865528 | 5.53E-65 | -1.69E-46 |
| MS.gene049756 | MS.gene68873 | 0.812306 | 4.53E-51 | -1.69E-46 |
| MS.gene049758 | MS.gene68873 | 0.850445 | 1.69E-60 | -1.69E-46 |
| MS.gene04984 | MS.gene68873 | 0.827682 | 1.37E-54 | -1.69E-46 |
| MS.gene049910 | MS.gene68873 | 0.817531 | 3.14E-52 | -1.69E-46 |
| MS.gene049915 | MS.gene68873 | 0.822037 | 2.93E-53 | -1.69E-46 |
| MS.gene050226 | MS.gene68873 | 0.872529 | 2.96E-67 | -1.69E-46 |
| MS.gene050227 | MS.gene68873 | 0.883071 | 6.12E-71 | -1.69E-46 |
| MS.gene050228 | MS.gene68873 | 0.878457 | 2.76E-69 | -1.69E-46 |
| MS.gene050303 | MS.gene68873 | 0.854181 | 1.46E-61 | -1.69E-46 |
| MS.gene050330 | MS.gene68873 | -0.818032 | 2.42E-52 | -1.69E-46 |
| MS.gene050438 | MS.gene68873 | 0.815553 | 8.72E-52 | -1.69E-46 |
| MS.gene050442 | MS.gene68873 | 0.845041 | 5.20E-59 | -1.69E-46 |
| MS.gene050558 | MS.gene68873 | 0.802243 | 6.15E-49 | -1.69E-46 |
| MS.gene050623 | MS.gene68873 | -0.892131 | 2.11E-74 | -1.69E-46 |
| MS.gene050642 | MS.gene68873 | 0.887373 | 1.51E-72 | -1.69E-46 |
| MS.gene050719 | MS.gene68873 | 0.85253 | 4.35E-61 | -1.69E-46 |
| MS.gene050721 | MS.gene68873 | 0.850669 | 1.46E-60 | -1.69E-46 |
| MS.gene050724 | MS.gene68873 | 0.844525 | 7.16E-59 | -1.69E-46 |
| MS.gene050750 | MS.gene68873 | 0.829537 | 4.87E-55 | -1.69E-46 |
| MS.gene050762 | MS.gene68873 | 0.821363 | 4.20E-53 | -1.69E-46 |
| MS.gene050763 | MS.gene68873 | 0.822729 | 2.03E-53 | -1.69E-46 |
| MS.gene050815 | MS.gene68873 | 0.809889 | 1.51E-50 | -1.69E-46 |
| MS.gene050820 | MS.gene68873 | 0.829791 | 4.23E-55 | -1.69E-46 |
| MS.gene050824 | MS.gene68873 | 0.823104 | 1.66E-53 | -1.69E-46 |
| MS.gene050953 | MS.gene68873 | 0.828726 | 7.66E-55 | -1.69E-46 |
| MS.gene051088 | MS.gene68873 | 0.811195 | 7.91E-51 | -1.69E-46 |
| MS.gene051243 | MS.gene68873 | 0.809916 | 1.49E-50 | -1.69E-46 |
| MS.gene051257 | MS.gene68873 | 0.810648 | 1.04E-50 | -1.69E-46 |
| MS.gene051349 | MS.gene68873 | 0.809517 | 1.82E-50 | -1.69E-46 |
| MS.gene051535 | MS.gene68873 | 0.804523 | 2.07E-49 | -1.69E-46 |
| MS.gene051633 | MS.gene68873 | 0.800952 | 1.13E-48 | -1.69E-46 |
| MS.gene051662 | MS.gene68873 | 0.82638 | 2.80E-54 | -1.69E-46 |
| MS.gene051663 | MS.gene68873 | 0.817956 | 2.52E-52 | -1.69E-46 |
| MS.gene051757 | MS.gene68873 | 0.80375 | 3.00E-49 | -1.69E-46 |
| MS.gene051860 | MS.gene68873 | 0.845562 | 3.76E-59 | -1.69E-46 |
| MS.gene051983 | MS.gene68873 | 0.86639 | 2.95E-65 | -1.69E-46 |
| MS.gene052012 | MS.gene68873 | 0.810024 | 1.42E-50 | -1.69E-46 |
| MS.gene052077 | MS.gene68873 | 0.829465 | 5.07E-55 | -1.69E-46 |
| MS.gene052176 | MS.gene68873 | 0.87887 | 1.98E-69 | -1.69E-46 |
| MS.gene052302 | MS.gene68873 | 0.855497 | 6.06E-62 | -1.69E-46 |
| MS.gene052309 | MS.gene68873 | -0.849717 | 2.70E-60 | -1.69E-46 |
| MS.gene052376 | MS.gene68873 | 0.869024 | 4.22E-66 | -1.69E-46 |
| MS.gene052389 | MS.gene68873 | 0.827949 | 1.18E-54 | -1.69E-46 |
| MS.gene052600 | MS.gene68873 | 0.819647 | 1.04E-52 | -1.69E-46 |
| MS.gene052856 | MS.gene68873 | 0.82807 | 1.10E-54 | -1.69E-46 |
| MS.gene052858 | MS.gene68873 | 0.824349 | 8.47E-54 | -1.69E-46 |
| MS.gene052860 | MS.gene68873 | 0.821204 | 4.57E-53 | -1.69E-46 |
| MS.gene053216 | MS.gene68873 | 0.814727 | 1.33E-51 | -1.69E-46 |
| MS.gene053836 | MS.gene68873 | 0.873984 | 9.63E-68 | -1.69E-46 |
| MS.gene054184 | MS.gene68873 | 0.810597 | 1.07E-50 | -1.69E-46 |
| MS.gene054185 | MS.gene68873 | 0.819591 | 1.07E-52 | -1.69E-46 |
| MS.gene054379 | MS.gene68873 | 0.823879 | 1.09E-53 | -1.69E-46 |
| MS.gene054405 | MS.gene68873 | 0.816233 | 6.15E-52 | -1.69E-46 |
| MS.gene054468 | MS.gene68873 | 0.806857 | 6.71E-50 | -1.69E-46 |
| MS.gene054472 | MS.gene68873 | 0.802081 | 6.64E-49 | -1.69E-46 |
| MS.gene054473 | MS.gene68873 | 0.804193 | 2.43E-49 | -1.69E-46 |
| MS.gene054542 | MS.gene68873 | 0.804703 | 1.90E-49 | -1.69E-46 |
| MS.gene054658 | MS.gene68873 | 0.800654 | 1.30E-48 | -1.69E-46 |
| MS.gene054667 | MS.gene68873 | 0.812709 | 3.70E-51 | -1.69E-46 |
| MS.gene054930 | MS.gene68873 | 0.85983 | 3.15E-63 | -1.69E-46 |
| MS.gene054942 | MS.gene68873 | 0.848054 | 7.82E-60 | -1.69E-46 |
| MS.gene054983 | MS.gene68873 | 0.845632 | 3.60E-59 | -1.69E-46 |
| MS.gene05510 | MS.gene68873 | 0.81402 | 1.91E-51 | -1.69E-46 |
| MS.gene055422 | MS.gene68873 | 0.806306 | 8.77E-50 | -1.69E-46 |
| MS.gene055428 | MS.gene68873 | 0.801833 | 7.47E-49 | -1.69E-46 |
| MS.gene055441 | MS.gene68873 | 0.800456 | 1.43E-48 | -1.69E-46 |
| MS.gene055701 | MS.gene68873 | 0.804958 | 1.68E-49 | -1.69E-46 |
| MS.gene055816 | MS.gene68873 | -0.805649 | 1.21E-49 | -1.69E-46 |
| MS.gene056069 | MS.gene68873 | -0.819925 | 8.99E-53 | -1.69E-46 |
| MS.gene056087 | MS.gene68873 | 0.815018 | 1.15E-51 | -1.69E-46 |
| MS.gene056400 | MS.gene68873 | 0.827397 | 1.60E-54 | -1.69E-46 |
| MS.gene056506 | MS.gene68873 | 0.826034 | 3.39E-54 | -1.69E-46 |
| MS.gene056552 | MS.gene68873 | 0.808122 | 3.62E-50 | -1.69E-46 |
| MS.gene056631 | MS.gene68873 | 0.802676 | 5.01E-49 | -1.69E-46 |
| MS.gene056875 | MS.gene68873 | 0.82071 | 5.94E-53 | -1.69E-46 |
| MS.gene056979 | MS.gene68873 | 0.854647 | 1.07E-61 | -1.69E-46 |
| MS.gene057060 | MS.gene68873 | 0.801556 | 8.52E-49 | -1.69E-46 |
| MS.gene057079 | MS.gene68873 | 0.813701 | 2.24E-51 | -1.69E-46 |
| MS.gene057121 | MS.gene68873 | 0.82061 | 6.26E-53 | -1.69E-46 |
| MS.gene057140 | MS.gene68873 | 0.800453 | 1.43E-48 | -1.69E-46 |
| MS.gene057260 | MS.gene68873 | 0.827862 | 1.24E-54 | -1.69E-46 |
| MS.gene057439 | MS.gene68873 | 0.805208 | 1.49E-49 | -1.69E-46 |
| MS.gene05761 | MS.gene68873 | 0.806532 | 7.86E-50 | -1.69E-46 |
| MS.gene057967 | MS.gene68873 | 0.825923 | 3.60E-54 | -1.69E-46 |
| MS.gene058002 | MS.gene68873 | 0.806059 | 9.89E-50 | -1.69E-46 |
| MS.gene058084 | MS.gene68873 | 0.842874 | 1.98E-58 | -1.69E-46 |
| MS.gene058139 | MS.gene68873 | 0.823124 | 1.64E-53 | -1.69E-46 |
| MS.gene058142 | MS.gene68873 | 0.808959 | 2.40E-50 | -1.69E-46 |
| MS.gene058146 | MS.gene68873 | 0.818482 | 1.92E-52 | -1.69E-46 |
| MS.gene058168 | MS.gene68873 | 0.811195 | 7.90E-51 | -1.69E-46 |
| MS.gene058182 | MS.gene68873 | 0.839432 | 1.59E-57 | -1.69E-46 |
| MS.gene058238 | MS.gene68873 | 0.809183 | 2.15E-50 | -1.69E-46 |
| MS.gene058399 | MS.gene68873 | 0.832985 | 6.91E-56 | -1.69E-46 |
| MS.gene058435 | MS.gene68873 | 0.806973 | 6.34E-50 | -1.69E-46 |
| MS.gene058549 | MS.gene68873 | 0.824828 | 6.53E-54 | -1.69E-46 |
| MS.gene058710 | MS.gene68873 | 0.821803 | 3.32E-53 | -1.69E-46 |
| MS.gene058749 | MS.gene68873 | 0.839329 | 1.69E-57 | -1.69E-46 |
| MS.gene058833 | MS.gene68873 | 0.80983 | 1.56E-50 | -1.69E-46 |
| MS.gene058906 | MS.gene68873 | 0.814959 | 1.18E-51 | -1.69E-46 |
| MS.gene059124 | MS.gene68873 | 0.811054 | 8.48E-51 | -1.69E-46 |
| MS.gene059249 | MS.gene68873 | 0.860889 | 1.51E-63 | -1.69E-46 |
| MS.gene05954 | MS.gene68873 | 0.813289 | 2.76E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene68873 | 0.818915 | 1.53E-52 | -1.69E-46 |
| MS.gene05979 | MS.gene68873 | 0.820549 | 6.47E-53 | -1.69E-46 |
| MS.gene059830 | MS.gene68873 | 0.802896 | 4.51E-49 | -1.69E-46 |
| MS.gene05984 | MS.gene68873 | 0.857615 | 1.45E-62 | -1.69E-46 |
| MS.gene059899 | MS.gene68873 | 0.814769 | 1.30E-51 | -1.69E-46 |
| MS.gene060084 | MS.gene68873 | 0.823573 | 1.29E-53 | -1.69E-46 |
| MS.gene060165 | MS.gene68873 | 0.820731 | 5.88E-53 | -1.69E-46 |
| MS.gene060297 | MS.gene68873 | 0.834633 | 2.68E-56 | -1.69E-46 |
| MS.gene060404 | MS.gene68873 | 0.800975 | 1.12E-48 | -1.69E-46 |
| MS.gene060476 | MS.gene68873 | 0.815471 | 9.09E-52 | -1.69E-46 |
| MS.gene060572 | MS.gene68873 | 0.835995 | 1.21E-56 | -1.69E-46 |
| MS.gene060586 | MS.gene68873 | 0.806563 | 7.74E-50 | -1.69E-46 |
| MS.gene060619 | MS.gene68873 | 0.82473 | 6.89E-54 | -1.69E-46 |
| MS.gene060683 | MS.gene68873 | 0.820579 | 6.37E-53 | -1.69E-46 |
| MS.gene060685 | MS.gene68873 | 0.812571 | 3.96E-51 | -1.69E-46 |
| MS.gene060802 | MS.gene68873 | 0.82154 | 3.83E-53 | -1.69E-46 |
| MS.gene060803 | MS.gene68873 | 0.845204 | 4.70E-59 | -1.69E-46 |
| MS.gene060907 | MS.gene68873 | 0.818999 | 1.46E-52 | -1.69E-46 |
| MS.gene060998 | MS.gene68873 | 0.812421 | 4.27E-51 | -1.69E-46 |
| MS.gene061040 | MS.gene68873 | -0.847956 | 8.33E-60 | -1.69E-46 |
| MS.gene061049 | MS.gene68873 | 0.802561 | 5.29E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68873.t1 | MTR_4g132780 | 98.930 | 187 | 2 | 0 | 1 | 187 | 582 | 768 | 3.08e-127 | 375 |
| MS.gene68873.t1 | MTR_2g025130 | 46.199 | 171 | 45 | 4 | 1 | 171 | 326 | 449 | 7.68e-37 | 133 |
| MS.gene68873.t1 | MTR_2g451540 | 40.000 | 160 | 67 | 2 | 2 | 158 | 538 | 671 | 7.97e-30 | 115 |
| MS.gene68873.t1 | MTR_1g012840 | 42.038 | 157 | 78 | 2 | 2 | 158 | 515 | 658 | 4.72e-29 | 113 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68873.t1 | AT1G62390 | 67.630 | 173 | 49 | 2 | 1 | 171 | 584 | 751 | 4.14e-67 | 218 |
| MS.gene68873.t1 | AT2G25290 | 38.854 | 157 | 83 | 2 | 2 | 158 | 543 | 686 | 1.19e-27 | 109 |
| MS.gene68873.t1 | AT2G25290 | 38.854 | 157 | 83 | 2 | 2 | 158 | 543 | 686 | 1.19e-27 | 109 |
| MS.gene68873.t1 | AT2G25290 | 38.854 | 157 | 83 | 2 | 2 | 158 | 543 | 686 | 1.19e-27 | 109 |
| MS.gene68873.t1 | AT2G25290 | 38.854 | 157 | 83 | 2 | 2 | 158 | 543 | 686 | 1.19e-27 | 109 |
| MS.gene68873.t1 | AT4G32070 | 38.217 | 157 | 84 | 2 | 2 | 158 | 580 | 723 | 6.94e-26 | 103 |
| MS.gene68873.t1 | AT4G32070 | 38.217 | 157 | 84 | 2 | 2 | 158 | 580 | 723 | 6.94e-26 | 103 |
| MS.gene68873.t1 | AT5G20360 | 27.607 | 163 | 101 | 2 | 2 | 164 | 602 | 747 | 1.44e-16 | 77.4 |
| MS.gene68873.t1 | AT5G20360 | 27.607 | 163 | 101 | 2 | 2 | 164 | 612 | 757 | 1.57e-16 | 77.0 |
| MS.gene68873.t1 | AT5G20360 | 27.607 | 163 | 101 | 2 | 2 | 164 | 612 | 757 | 1.57e-16 | 77.0 |
| MS.gene68873.t1 | AT5G20360 | 28.221 | 163 | 100 | 2 | 2 | 164 | 552 | 697 | 1.67e-16 | 77.0 |
| MS.gene68873.t1 | AT5G20360 | 28.221 | 163 | 100 | 2 | 2 | 164 | 552 | 697 | 1.67e-16 | 77.0 |
| MS.gene68873.t1 | AT5G20360 | 28.221 | 163 | 100 | 2 | 2 | 164 | 522 | 667 | 1.69e-16 | 77.0 |
| MS.gene68873.t1 | AT5G20360 | 28.221 | 163 | 100 | 2 | 2 | 164 | 522 | 667 | 1.69e-16 | 77.0 |
Find 49 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGAGAAGAAGAAAGAAAC+AGG | 0.268964 | 8.4:+1539484 | MS.gene68873:CDS |
| TCCCAAGTGGAATGCAAATT+AGG | 0.293568 | 8.4:+1539638 | MS.gene68873:CDS |
| TCTTCTTTCTTTGTTGCAGT+TGG | 0.304943 | 8.4:-1539458 | None:intergenic |
| CTCTAGCTCATCGGTCTTGT+TGG | 0.326163 | 8.4:-1539810 | None:intergenic |
| GTCTTCTGTCGTTGGAGGTC+AGG | 0.334086 | 8.4:+1539526 | MS.gene68873:CDS |
| TGCAAAAGACCAATGGAGTT+TGG | 0.335622 | 8.4:-1539297 | None:intergenic |
| ACAGAACGCTTTAAGCTTGC+AGG | 0.338073 | 8.4:+1539695 | MS.gene68873:CDS |
| CTTCTTTCTTTGTTGCAGTT+GGG | 0.350219 | 8.4:-1539457 | None:intergenic |
| TCATTTATCTCATCCTTGAT+TGG | 0.361253 | 8.4:-1539846 | None:intergenic |
| TACAAAAGGTGATGATAAAA+AGG | 0.419380 | 8.4:+1539772 | MS.gene68873:CDS |
| CCGTTGGATCCCATGTTGAC+AGG | 0.437619 | 8.4:-1539337 | None:intergenic |
| TCTAGCTCATCGGTCTTGTT+GGG | 0.448987 | 8.4:-1539809 | None:intergenic |
| TCTTGTTGGGAAGTGGACTT+TGG | 0.460672 | 8.4:-1539796 | None:intergenic |
| TTCCTAATTTGCATTCCACT+TGG | 0.481107 | 8.4:-1539640 | None:intergenic |
| TACTGATATGTGGGAGAAGT+TGG | 0.484531 | 8.4:+1539406 | MS.gene68873:CDS |
| TCTTCTGTCGTTGGAGGTCA+GGG | 0.496390 | 8.4:+1539527 | MS.gene68873:CDS |
| AGCGTTCTGTTGCAGCATCC+AGG | 0.499132 | 8.4:-1539682 | None:intergenic |
| GGCGAGTCTTCTGTCGTTGG+AGG | 0.503882 | 8.4:+1539521 | MS.gene68873:CDS |
| TTGAAATGGCCAAACTCCAT+TGG | 0.504188 | 8.4:+1539288 | None:intergenic |
| AACAGTTGAAGAGTTTCCGT+TGG | 0.508342 | 8.4:-1539353 | None:intergenic |
| ATCAACAAGGAGGCCAATCA+AGG | 0.509255 | 8.4:+1539833 | MS.gene68873:CDS |
| TCATCGGTCTTGTTGGGAAG+TGG | 0.515199 | 8.4:-1539803 | None:intergenic |
| TCCTAATTTGCATTCCACTT+GGG | 0.517971 | 8.4:-1539639 | None:intergenic |
| GCAAATTAGGAATGGATGGT+TGG | 0.521832 | 8.4:+1539651 | MS.gene68873:CDS |
| AGCTCAAACGGTGATACAAA+AGG | 0.525012 | 8.4:+1539758 | MS.gene68873:CDS |
| ACAGAGGGCAAAGGAGCTAA+AGG | 0.535384 | 8.4:+1539433 | MS.gene68873:CDS |
| TGAAATCTGCTACTGATATG+TGG | 0.535440 | 8.4:+1539396 | MS.gene68873:CDS |
| AACGACAGAAGACTCGCCTT+CGG | 0.538485 | 8.4:-1539516 | None:intergenic |
| TGATATGTGGGAGAAGTTGG+AGG | 0.542485 | 8.4:+1539409 | MS.gene68873:CDS |
| CTTGTTGATCTCTAGCTCAT+CGG | 0.544542 | 8.4:-1539819 | None:intergenic |
| CTAAAGAATCACAGCTCAAA+CGG | 0.544765 | 8.4:+1539746 | MS.gene68873:CDS |
| TAGCAATTGCAAAAGACCAA+TGG | 0.553654 | 8.4:-1539304 | None:intergenic |
| GAAGGCGAGTCTTCTGTCGT+TGG | 0.569947 | 8.4:+1539518 | MS.gene68873:CDS |
| TAAGCTTGCAGGAGCTTCTG+AGG | 0.573931 | 8.4:+1539706 | MS.gene68873:CDS |
| AGAAGATAGACCTGTCAACA+TGG | 0.574294 | 8.4:+1539327 | MS.gene68873:CDS |
| TTGAGAAGAAGAAAGAAACA+GGG | 0.574394 | 8.4:+1539485 | MS.gene68873:CDS |
| AGTGGAATGCAAATTAGGAA+TGG | 0.579074 | 8.4:+1539643 | MS.gene68873:CDS |
| GAAATCTGCTACTGATATGT+GGG | 0.582332 | 8.4:+1539397 | MS.gene68873:CDS |
| GGAGAAGTTGGAGGAACAGA+GGG | 0.591263 | 8.4:+1539418 | MS.gene68873:CDS |
| GTTGGAGGAACAGAGGGCAA+AGG | 0.601122 | 8.4:+1539424 | MS.gene68873:CDS |
| GAATGCAAATTAGGAATGGA+TGG | 0.608405 | 8.4:+1539647 | MS.gene68873:CDS |
| TCAACTGTTTAACAGTGCAG+AGG | 0.632330 | 8.4:+1539367 | MS.gene68873:CDS |
| CCTGTCAACATGGGATCCAA+CGG | 0.635538 | 8.4:+1539337 | MS.gene68873:CDS |
| AGAAGCTGCTGAGCAAGCAG+TGG | 0.642416 | 8.4:+1539565 | MS.gene68873:CDS |
| CGATGAGCTAGAGATCAACA+AGG | 0.643060 | 8.4:+1539820 | MS.gene68873:CDS |
| GAAGATAGACCTGTCAACAT+GGG | 0.646417 | 8.4:+1539328 | MS.gene68873:CDS |
| GGGAGAAGTTGGAGGAACAG+AGG | 0.662089 | 8.4:+1539417 | MS.gene68873:CDS |
| AAACAGGGAAGTGCTACCGA+AGG | 0.674314 | 8.4:+1539500 | MS.gene68873:CDS |
| TGAGCTAGAGATCAACAAGG+AGG | 0.759563 | 8.4:+1539823 | MS.gene68873:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATCACAAATTCATCTTTTTT+GGG | + | chr8.4:1539595-1539614 | MS.gene68873:CDS | 20.0% |
| ! | TACAAAAGGTGATGATAAAA+AGG | + | chr8.4:1539772-1539791 | MS.gene68873:CDS | 25.0% |
| !!! | GATCACAAATTCATCTTTTT+TGG | + | chr8.4:1539594-1539613 | MS.gene68873:CDS | 25.0% |
| !!! | TCACAAATTCATCTTTTTTG+GGG | + | chr8.4:1539596-1539615 | MS.gene68873:CDS | 25.0% |
| TTGAGAAGAAGAAAGAAACA+GGG | + | chr8.4:1539485-1539504 | MS.gene68873:CDS | 30.0% | |
| AGTGGAATGCAAATTAGGAA+TGG | + | chr8.4:1539643-1539662 | MS.gene68873:CDS | 35.0% | |
| CTAAAGAATCACAGCTCAAA+CGG | + | chr8.4:1539746-1539765 | MS.gene68873:CDS | 35.0% | |
| CTTCTTTCTTTGTTGCAGTT+GGG | - | chr8.4:1539460-1539479 | None:intergenic | 35.0% | |
| GAAATCTGCTACTGATATGT+GGG | + | chr8.4:1539397-1539416 | MS.gene68873:CDS | 35.0% | |
| GAATGCAAATTAGGAATGGA+TGG | + | chr8.4:1539647-1539666 | MS.gene68873:CDS | 35.0% | |
| GTTGAGAAGAAGAAAGAAAC+AGG | + | chr8.4:1539484-1539503 | MS.gene68873:CDS | 35.0% | |
| TAGCAATTGCAAAAGACCAA+TGG | - | chr8.4:1539307-1539326 | None:intergenic | 35.0% | |
| TCTTCTTTCTTTGTTGCAGT+TGG | - | chr8.4:1539461-1539480 | None:intergenic | 35.0% | |
| TGAAATCTGCTACTGATATG+TGG | + | chr8.4:1539396-1539415 | MS.gene68873:CDS | 35.0% | |
| ! | TCCTAATTTGCATTCCACTT+GGG | - | chr8.4:1539642-1539661 | None:intergenic | 35.0% |
| ! | TTCCTAATTTGCATTCCACT+TGG | - | chr8.4:1539643-1539662 | None:intergenic | 35.0% |
| !!! | TTCTGAGGCAGATATTTTGA+TGG | + | chr8.4:1539721-1539740 | MS.gene68873:CDS | 35.0% |
| AACAGTTGAAGAGTTTCCGT+TGG | - | chr8.4:1539356-1539375 | None:intergenic | 40.0% | |
| AGAAGATAGACCTGTCAACA+TGG | + | chr8.4:1539327-1539346 | MS.gene68873:CDS | 40.0% | |
| AGCTCAAACGGTGATACAAA+AGG | + | chr8.4:1539758-1539777 | MS.gene68873:CDS | 40.0% | |
| GAAGATAGACCTGTCAACAT+GGG | + | chr8.4:1539328-1539347 | MS.gene68873:CDS | 40.0% | |
| GCAAATTAGGAATGGATGGT+TGG | + | chr8.4:1539651-1539670 | MS.gene68873:CDS | 40.0% | |
| TACTGATATGTGGGAGAAGT+TGG | + | chr8.4:1539406-1539425 | MS.gene68873:CDS | 40.0% | |
| TCAACTGTTTAACAGTGCAG+AGG | + | chr8.4:1539367-1539386 | MS.gene68873:CDS | 40.0% | |
| TCCCAAGTGGAATGCAAATT+AGG | + | chr8.4:1539638-1539657 | MS.gene68873:CDS | 40.0% | |
| TGCAAAAGACCAATGGAGTT+TGG | - | chr8.4:1539300-1539319 | None:intergenic | 40.0% | |
| ! | CTTGTTGATCTCTAGCTCAT+CGG | - | chr8.4:1539822-1539841 | None:intergenic | 40.0% |
| ! | GCTTTTCGAAAAATCCCAAG+TGG | + | chr8.4:1539625-1539644 | MS.gene68873:CDS | 40.0% |
| ATCAACAAGGAGGCCAATCA+AGG | + | chr8.4:1539833-1539852 | MS.gene68873:CDS | 45.0% | |
| CGATGAGCTAGAGATCAACA+AGG | + | chr8.4:1539820-1539839 | MS.gene68873:CDS | 45.0% | |
| GGATGGTTGGAAGAAAAACC+TGG | + | chr8.4:1539664-1539683 | MS.gene68873:CDS | 45.0% | |
| TGAGCTAGAGATCAACAAGG+AGG | + | chr8.4:1539823-1539842 | MS.gene68873:CDS | 45.0% | |
| TGATATGTGGGAGAAGTTGG+AGG | + | chr8.4:1539409-1539428 | MS.gene68873:CDS | 45.0% | |
| ! | ACAGAACGCTTTAAGCTTGC+AGG | + | chr8.4:1539695-1539714 | MS.gene68873:CDS | 45.0% |
| ! | TCTTGTTGGGAAGTGGACTT+TGG | - | chr8.4:1539799-1539818 | None:intergenic | 45.0% |
| !! | TCTAGCTCATCGGTCTTGTT+GGG | - | chr8.4:1539812-1539831 | None:intergenic | 45.0% |
| AACGACAGAAGACTCGCCTT+CGG | - | chr8.4:1539519-1539538 | None:intergenic | 50.0% | |
| ACAGAGGGCAAAGGAGCTAA+AGG | + | chr8.4:1539433-1539452 | MS.gene68873:CDS | 50.0% | |
| CCTGTCAACATGGGATCCAA+CGG | + | chr8.4:1539337-1539356 | MS.gene68873:CDS | 50.0% | |
| GGAGAAGTTGGAGGAACAGA+GGG | + | chr8.4:1539418-1539437 | MS.gene68873:CDS | 50.0% | |
| TAAGCTTGCAGGAGCTTCTG+AGG | + | chr8.4:1539706-1539725 | MS.gene68873:CDS | 50.0% | |
| TCTTCTGTCGTTGGAGGTCA+GGG | + | chr8.4:1539527-1539546 | MS.gene68873:CDS | 50.0% | |
| ! | CTCTAGCTCATCGGTCTTGT+TGG | - | chr8.4:1539813-1539832 | None:intergenic | 50.0% |
| !! | AAACAGGGAAGTGCTACCGA+AGG | + | chr8.4:1539500-1539519 | MS.gene68873:CDS | 50.0% |
| !! | TCATCGGTCTTGTTGGGAAG+TGG | - | chr8.4:1539806-1539825 | None:intergenic | 50.0% |
| AGAAGCTGCTGAGCAAGCAG+TGG | + | chr8.4:1539565-1539584 | MS.gene68873:CDS | 55.0% | |
| AGCGTTCTGTTGCAGCATCC+AGG | - | chr8.4:1539685-1539704 | None:intergenic | 55.0% | |
| CCGTTGGATCCCATGTTGAC+AGG | - | chr8.4:1539340-1539359 | None:intergenic | 55.0% | |
| GAAGGCGAGTCTTCTGTCGT+TGG | + | chr8.4:1539518-1539537 | MS.gene68873:CDS | 55.0% | |
| GGGAGAAGTTGGAGGAACAG+AGG | + | chr8.4:1539417-1539436 | MS.gene68873:CDS | 55.0% | |
| GTCTTCTGTCGTTGGAGGTC+AGG | + | chr8.4:1539526-1539545 | MS.gene68873:CDS | 55.0% | |
| GTTGGAGGAACAGAGGGCAA+AGG | + | chr8.4:1539424-1539443 | MS.gene68873:CDS | 55.0% | |
| GGCGAGTCTTCTGTCGTTGG+AGG | + | chr8.4:1539521-1539540 | MS.gene68873:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 1539293 | 1539859 | 1539293 | ID=MS.gene68873 |
| chr8.4 | mRNA | 1539293 | 1539859 | 1539293 | ID=MS.gene68873.t1;Parent=MS.gene68873 |
| chr8.4 | exon | 1539293 | 1539859 | 1539293 | ID=MS.gene68873.t1.exon1;Parent=MS.gene68873.t1 |
| chr8.4 | CDS | 1539293 | 1539859 | 1539293 | ID=cds.MS.gene68873.t1;Parent=MS.gene68873.t1 |
| Gene Sequence |
| Protein sequence |
