Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77349.t1 | XP_013449135.1 | 86.9 | 145 | 18 | 1 | 1 | 145 | 106 | 249 | 5.40E-68 | 266.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77349.t1 | Q9SRM5 | 52.4 | 145 | 68 | 1 | 1 | 144 | 111 | 255 | 1.1e-42 | 174.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77349.t1 | A0A072U0G5 | 86.9 | 145 | 18 | 1 | 1 | 145 | 106 | 249 | 3.9e-68 | 266.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene77349 | -0.802085 | 6.63E-49 | -1.69E-46 |
| MS.gene049171 | MS.gene77349 | -0.845296 | 4.44E-59 | -1.69E-46 |
| MS.gene049217 | MS.gene77349 | -0.80398 | 2.69E-49 | -1.69E-46 |
| MS.gene049222 | MS.gene77349 | -0.809615 | 1.73E-50 | -1.69E-46 |
| MS.gene049285 | MS.gene77349 | -0.820919 | 5.32E-53 | -1.69E-46 |
| MS.gene049320 | MS.gene77349 | -0.824322 | 8.60E-54 | -1.69E-46 |
| MS.gene04932 | MS.gene77349 | 0.825129 | 5.55E-54 | -1.69E-46 |
| MS.gene049549 | MS.gene77349 | 0.835682 | 1.46E-56 | -1.69E-46 |
| MS.gene049609 | MS.gene77349 | -0.814633 | 1.39E-51 | -1.69E-46 |
| MS.gene049611 | MS.gene77349 | 0.853569 | 2.19E-61 | -1.69E-46 |
| MS.gene049633 | MS.gene77349 | -0.80808 | 3.69E-50 | -1.69E-46 |
| MS.gene049634 | MS.gene77349 | 0.832465 | 9.31E-56 | -1.69E-46 |
| MS.gene050163 | MS.gene77349 | -0.815608 | 8.47E-52 | -1.69E-46 |
| MS.gene050165 | MS.gene77349 | -0.817785 | 2.75E-52 | -1.69E-46 |
| MS.gene050170 | MS.gene77349 | -0.813312 | 2.73E-51 | -1.69E-46 |
| MS.gene050323 | MS.gene77349 | -0.809691 | 1.67E-50 | -1.69E-46 |
| MS.gene050333 | MS.gene77349 | -0.820159 | 7.95E-53 | -1.69E-46 |
| MS.gene050338 | MS.gene77349 | 0.823252 | 1.53E-53 | -1.69E-46 |
| MS.gene050381 | MS.gene77349 | 0.815699 | 8.09E-52 | -1.69E-46 |
| MS.gene050444 | MS.gene77349 | -0.832183 | 1.09E-55 | -1.69E-46 |
| MS.gene050496 | MS.gene77349 | -0.819183 | 1.33E-52 | -1.69E-46 |
| MS.gene050504 | MS.gene77349 | -0.83537 | 1.75E-56 | -1.69E-46 |
| MS.gene050577 | MS.gene77349 | -0.827433 | 1.57E-54 | -1.69E-46 |
| MS.gene050612 | MS.gene77349 | -0.848118 | 7.51E-60 | -1.69E-46 |
| MS.gene050647 | MS.gene77349 | 0.809497 | 1.84E-50 | -1.69E-46 |
| MS.gene050675 | MS.gene77349 | -0.83386 | 4.18E-56 | -1.69E-46 |
| MS.gene050699 | MS.gene77349 | -0.818396 | 2.00E-52 | -1.69E-46 |
| MS.gene050772 | MS.gene77349 | -0.828662 | 7.94E-55 | -1.69E-46 |
| MS.gene05079 | MS.gene77349 | 0.831873 | 1.30E-55 | -1.69E-46 |
| MS.gene050809 | MS.gene77349 | 0.815287 | 9.99E-52 | -1.69E-46 |
| MS.gene05080 | MS.gene77349 | -0.804437 | 2.16E-49 | -1.69E-46 |
| MS.gene050833 | MS.gene77349 | 0.808117 | 3.63E-50 | -1.69E-46 |
| MS.gene050868 | MS.gene77349 | -0.815761 | 7.83E-52 | -1.69E-46 |
| MS.gene050871 | MS.gene77349 | -0.815093 | 1.10E-51 | -1.69E-46 |
| MS.gene050904 | MS.gene77349 | -0.804271 | 2.34E-49 | -1.69E-46 |
| MS.gene050986 | MS.gene77349 | -0.80447 | 2.13E-49 | -1.69E-46 |
| MS.gene05104 | MS.gene77349 | 0.846518 | 2.07E-59 | -1.69E-46 |
| MS.gene051127 | MS.gene77349 | 0.815417 | 9.34E-52 | -1.69E-46 |
| MS.gene051131 | MS.gene77349 | 0.815926 | 7.20E-52 | -1.69E-46 |
| MS.gene051138 | MS.gene77349 | 0.840533 | 8.21E-58 | -1.69E-46 |
| MS.gene051317 | MS.gene77349 | -0.807358 | 5.26E-50 | -1.69E-46 |
| MS.gene051364 | MS.gene77349 | 0.826402 | 2.77E-54 | -1.69E-46 |
| MS.gene051366 | MS.gene77349 | 0.829323 | 5.49E-55 | -1.69E-46 |
| MS.gene051422 | MS.gene77349 | 0.813183 | 2.91E-51 | -1.69E-46 |
| MS.gene051651 | MS.gene77349 | 0.829785 | 4.24E-55 | -1.69E-46 |
| MS.gene051749 | MS.gene77349 | 0.819424 | 1.17E-52 | -1.69E-46 |
| MS.gene051810 | MS.gene77349 | -0.805823 | 1.11E-49 | -1.69E-46 |
| MS.gene051858 | MS.gene77349 | -0.81014 | 1.34E-50 | -1.69E-46 |
| MS.gene051874 | MS.gene77349 | -0.82846 | 8.88E-55 | -1.69E-46 |
| MS.gene051907 | MS.gene77349 | 0.80332 | 3.69E-49 | -1.69E-46 |
| MS.gene051973 | MS.gene77349 | 0.816183 | 6.31E-52 | -1.69E-46 |
| MS.gene052129 | MS.gene77349 | 0.842452 | 2.56E-58 | -1.69E-46 |
| MS.gene052164 | MS.gene77349 | -0.815995 | 6.95E-52 | -1.69E-46 |
| MS.gene052211 | MS.gene77349 | 0.802727 | 4.89E-49 | -1.69E-46 |
| MS.gene05240 | MS.gene77349 | 0.846543 | 2.03E-59 | -1.69E-46 |
| MS.gene05244 | MS.gene77349 | 0.820617 | 6.24E-53 | -1.69E-46 |
| MS.gene052606 | MS.gene77349 | 0.825487 | 4.56E-54 | -1.69E-46 |
| MS.gene052702 | MS.gene77349 | 0.816608 | 5.07E-52 | -1.69E-46 |
| MS.gene052805 | MS.gene77349 | 0.824136 | 9.50E-54 | -1.69E-46 |
| MS.gene052870 | MS.gene77349 | -0.801844 | 7.43E-49 | -1.69E-46 |
| MS.gene052872 | MS.gene77349 | -0.819272 | 1.27E-52 | -1.69E-46 |
| MS.gene053031 | MS.gene77349 | 0.8169 | 4.36E-52 | -1.69E-46 |
| MS.gene053033 | MS.gene77349 | 0.803663 | 3.13E-49 | -1.69E-46 |
| MS.gene053203 | MS.gene77349 | -0.810889 | 9.21E-51 | -1.69E-46 |
| MS.gene053420 | MS.gene77349 | 0.846318 | 2.34E-59 | -1.69E-46 |
| MS.gene053479 | MS.gene77349 | -0.826561 | 2.54E-54 | -1.69E-46 |
| MS.gene053824 | MS.gene77349 | -0.815481 | 9.04E-52 | -1.69E-46 |
| MS.gene053916 | MS.gene77349 | -0.810934 | 9.00E-51 | -1.69E-46 |
| MS.gene054181 | MS.gene77349 | 0.822511 | 2.28E-53 | -1.69E-46 |
| MS.gene054290 | MS.gene77349 | 0.810143 | 1.33E-50 | -1.69E-46 |
| MS.gene054642 | MS.gene77349 | -0.816582 | 5.13E-52 | -1.69E-46 |
| MS.gene054706 | MS.gene77349 | 0.839741 | 1.32E-57 | -1.69E-46 |
| MS.gene05470 | MS.gene77349 | -0.825862 | 3.72E-54 | -1.69E-46 |
| MS.gene05483 | MS.gene77349 | 0.822217 | 2.67E-53 | -1.69E-46 |
| MS.gene054884 | MS.gene77349 | 0.816848 | 4.48E-52 | -1.69E-46 |
| MS.gene054954 | MS.gene77349 | -0.816785 | 4.62E-52 | -1.69E-46 |
| MS.gene054959 | MS.gene77349 | 0.809045 | 2.30E-50 | -1.69E-46 |
| MS.gene055154 | MS.gene77349 | 0.82102 | 5.04E-53 | -1.69E-46 |
| MS.gene055158 | MS.gene77349 | 0.813869 | 2.06E-51 | -1.69E-46 |
| MS.gene055206 | MS.gene77349 | -0.807026 | 6.18E-50 | -1.69E-46 |
| MS.gene055362 | MS.gene77349 | -0.821175 | 4.64E-53 | -1.69E-46 |
| MS.gene055502 | MS.gene77349 | -0.841198 | 5.49E-58 | -1.69E-46 |
| MS.gene055577 | MS.gene77349 | -0.836556 | 8.73E-57 | -1.69E-46 |
| MS.gene055748 | MS.gene77349 | -0.829626 | 4.63E-55 | -1.69E-46 |
| MS.gene055750 | MS.gene77349 | -0.814999 | 1.16E-51 | -1.69E-46 |
| MS.gene055751 | MS.gene77349 | -0.830717 | 2.51E-55 | -1.69E-46 |
| MS.gene05589 | MS.gene77349 | -0.838135 | 3.44E-57 | -1.69E-46 |
| MS.gene056224 | MS.gene77349 | 0.831244 | 1.86E-55 | -1.69E-46 |
| MS.gene056359 | MS.gene77349 | 0.806247 | 9.02E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene77349 | -0.838066 | 3.58E-57 | -1.69E-46 |
| MS.gene056590 | MS.gene77349 | 0.846968 | 1.56E-59 | -1.69E-46 |
| MS.gene056591 | MS.gene77349 | 0.846421 | 2.20E-59 | -1.69E-46 |
| MS.gene056657 | MS.gene77349 | -0.821369 | 4.19E-53 | -1.69E-46 |
| MS.gene056688 | MS.gene77349 | 0.812199 | 4.78E-51 | -1.69E-46 |
| MS.gene056692 | MS.gene77349 | -0.842543 | 2.43E-58 | -1.69E-46 |
| MS.gene056694 | MS.gene77349 | 0.803905 | 2.79E-49 | -1.69E-46 |
| MS.gene056697 | MS.gene77349 | 0.839693 | 1.36E-57 | -1.69E-46 |
| MS.gene056748 | MS.gene77349 | 0.819675 | 1.03E-52 | -1.69E-46 |
| MS.gene056856 | MS.gene77349 | 0.891889 | 2.63E-74 | -1.69E-46 |
| MS.gene056857 | MS.gene77349 | 0.82278 | 1.97E-53 | -1.69E-46 |
| MS.gene056988 | MS.gene77349 | 0.80052 | 1.39E-48 | -1.69E-46 |
| MS.gene057261 | MS.gene77349 | 0.833933 | 4.01E-56 | -1.69E-46 |
| MS.gene057544 | MS.gene77349 | 0.833521 | 5.08E-56 | -1.69E-46 |
| MS.gene057547 | MS.gene77349 | 0.813931 | 1.99E-51 | -1.69E-46 |
| MS.gene057549 | MS.gene77349 | 0.809229 | 2.10E-50 | -1.69E-46 |
| MS.gene057658 | MS.gene77349 | -0.815671 | 8.20E-52 | -1.69E-46 |
| MS.gene057768 | MS.gene77349 | 0.835442 | 1.67E-56 | -1.69E-46 |
| MS.gene057831 | MS.gene77349 | -0.807694 | 4.46E-50 | -1.69E-46 |
| MS.gene058067 | MS.gene77349 | 0.806079 | 9.79E-50 | -1.69E-46 |
| MS.gene058068 | MS.gene77349 | 0.825391 | 4.81E-54 | -1.69E-46 |
| MS.gene058099 | MS.gene77349 | 0.8282 | 1.03E-54 | -1.69E-46 |
| MS.gene058158 | MS.gene77349 | 0.822253 | 2.62E-53 | -1.69E-46 |
| MS.gene058175 | MS.gene77349 | 0.804819 | 1.80E-49 | -1.69E-46 |
| MS.gene058334 | MS.gene77349 | 0.825101 | 5.63E-54 | -1.69E-46 |
| MS.gene058338 | MS.gene77349 | -0.808474 | 3.04E-50 | -1.69E-46 |
| MS.gene05837 | MS.gene77349 | -0.826165 | 3.15E-54 | -1.69E-46 |
| MS.gene058419 | MS.gene77349 | -0.837421 | 5.25E-57 | -1.69E-46 |
| MS.gene058544 | MS.gene77349 | -0.821341 | 4.25E-53 | -1.69E-46 |
| MS.gene058603 | MS.gene77349 | 0.83423 | 3.38E-56 | -1.69E-46 |
| MS.gene058723 | MS.gene77349 | -0.831904 | 1.28E-55 | -1.69E-46 |
| MS.gene058773 | MS.gene77349 | -0.813154 | 2.95E-51 | -1.69E-46 |
| MS.gene058838 | MS.gene77349 | -0.851383 | 9.20E-61 | -1.69E-46 |
| MS.gene059022 | MS.gene77349 | -0.832271 | 1.04E-55 | -1.69E-46 |
| MS.gene059023 | MS.gene77349 | -0.840132 | 1.05E-57 | -1.69E-46 |
| MS.gene059315 | MS.gene77349 | 0.818723 | 1.69E-52 | -1.69E-46 |
| MS.gene059677 | MS.gene77349 | -0.836443 | 9.33E-57 | -1.69E-46 |
| MS.gene059718 | MS.gene77349 | -0.817009 | 4.12E-52 | -1.69E-46 |
| MS.gene059865 | MS.gene77349 | -0.828412 | 9.12E-55 | -1.69E-46 |
| MS.gene059902 | MS.gene77349 | -0.809224 | 2.10E-50 | -1.69E-46 |
| MS.gene059953 | MS.gene77349 | 0.836474 | 9.16E-57 | -1.69E-46 |
| MS.gene059955 | MS.gene77349 | 0.837159 | 6.13E-57 | -1.69E-46 |
| MS.gene06000 | MS.gene77349 | -0.826132 | 3.21E-54 | -1.69E-46 |
| MS.gene060087 | MS.gene77349 | 0.810064 | 1.39E-50 | -1.69E-46 |
| MS.gene060405 | MS.gene77349 | 0.829722 | 4.39E-55 | -1.69E-46 |
| MS.gene060593 | MS.gene77349 | 0.804533 | 2.06E-49 | -1.69E-46 |
| MS.gene060762 | MS.gene77349 | -0.83811 | 3.49E-57 | -1.69E-46 |
| MS.gene06077 | MS.gene77349 | -0.824979 | 6.02E-54 | -1.69E-46 |
| MS.gene060823 | MS.gene77349 | 0.823772 | 1.16E-53 | -1.69E-46 |
| MS.gene06084 | MS.gene77349 | 0.808813 | 2.58E-50 | -1.69E-46 |
| MS.gene060982 | MS.gene77349 | -0.83048 | 2.87E-55 | -1.69E-46 |
| MS.gene061044 | MS.gene77349 | 0.814001 | 1.92E-51 | -1.69E-46 |
| MS.gene061150 | MS.gene77349 | -0.829688 | 4.48E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77349.t1 | MTR_7g068470 | 86.897 | 145 | 18 | 1 | 1 | 145 | 106 | 249 | 1.69e-90 | 263 |
| MS.gene77349.t1 | MTR_7g068460 | 84.828 | 145 | 22 | 0 | 1 | 145 | 137 | 281 | 1.29e-89 | 262 |
| MS.gene77349.t1 | MTR_7g068500 | 84.138 | 145 | 23 | 0 | 1 | 145 | 136 | 280 | 1.10e-88 | 259 |
| MS.gene77349.t1 | MTR_7g068470 | 87.234 | 141 | 17 | 1 | 5 | 145 | 106 | 245 | 2.01e-88 | 258 |
| MS.gene77349.t1 | MTR_7g068450 | 62.937 | 143 | 52 | 1 | 3 | 144 | 112 | 254 | 1.13e-63 | 195 |
| MS.gene77349.t1 | MTR_4g016510 | 62.069 | 145 | 54 | 1 | 1 | 144 | 111 | 255 | 1.23e-61 | 191 |
| MS.gene77349.t1 | MTR_7g068530 | 62.500 | 144 | 52 | 2 | 1 | 144 | 111 | 252 | 2.74e-60 | 186 |
| MS.gene77349.t1 | MTR_3g070460 | 51.034 | 145 | 70 | 1 | 1 | 144 | 111 | 255 | 1.34e-52 | 167 |
| MS.gene77349.t1 | MTR_7g068520 | 56.552 | 145 | 13 | 1 | 1 | 145 | 60 | 154 | 2.89e-48 | 152 |
| MS.gene77349.t1 | MTR_7g068550 | 51.389 | 144 | 69 | 1 | 1 | 144 | 111 | 253 | 5.75e-47 | 152 |
| MS.gene77349.t1 | MTR_4g016500 | 43.056 | 144 | 34 | 3 | 1 | 144 | 331 | 426 | 1.79e-27 | 105 |
| MS.gene77349.t1 | MTR_4g105680 | 29.921 | 127 | 79 | 3 | 1 | 121 | 135 | 257 | 5.62e-14 | 67.4 |
| MS.gene77349.t1 | MTR_2g015500 | 31.250 | 96 | 63 | 2 | 6 | 98 | 120 | 215 | 6.83e-14 | 66.6 |
| MS.gene77349.t1 | MTR_2g015510 | 30.208 | 96 | 64 | 2 | 6 | 98 | 119 | 214 | 1.48e-12 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77349.t1 | AT3G11210 | 52.414 | 145 | 68 | 1 | 1 | 144 | 111 | 255 | 6.82e-54 | 170 |
| MS.gene77349.t1 | AT2G38180 | 52.419 | 124 | 50 | 3 | 1 | 122 | 111 | 227 | 8.82e-41 | 138 |
| MS.gene77349.t1 | AT5G62930 | 29.921 | 127 | 74 | 5 | 8 | 128 | 56 | 173 | 1.97e-15 | 69.7 |
| MS.gene77349.t1 | AT5G45920 | 36.082 | 97 | 59 | 2 | 5 | 98 | 115 | 211 | 4.86e-15 | 69.7 |
| MS.gene77349.t1 | AT5G45920 | 36.082 | 97 | 59 | 2 | 5 | 98 | 115 | 211 | 4.86e-15 | 69.7 |
| MS.gene77349.t1 | AT5G62930 | 29.134 | 127 | 75 | 4 | 8 | 128 | 114 | 231 | 5.29e-15 | 69.7 |
| MS.gene77349.t1 | AT5G62930 | 29.134 | 127 | 75 | 4 | 8 | 128 | 140 | 257 | 6.77e-15 | 69.7 |
Find 30 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGTTAATGCCAGCTGAATT+TGG | 0.274855 | 7.2:+35300782 | MS.gene77349:CDS |
| AATGAGCTCAGTGATGAATT+TGG | 0.285385 | 7.2:+35300431 | MS.gene77349:intron |
| TATGATATGGTTTCTCTTGA+TGG | 0.317939 | 7.2:+35300818 | MS.gene77349:CDS |
| AGAAGATTCACCTTATGATA+TGG | 0.335446 | 7.2:+35300805 | MS.gene77349:CDS |
| CGATCAACGAGGCACAAATT+AGG | 0.337436 | 7.2:+35300308 | MS.gene77349:CDS |
| AATATATTCAGAAGCGTGTT+TGG | 0.360952 | 7.2:+35300484 | MS.gene77349:CDS |
| AGTGGGAACCAAGTCTATAT+TGG | 0.407643 | 7.2:+35300759 | MS.gene77349:CDS |
| CACTCTACATCTTGAGGAAA+AGG | 0.437938 | 7.2:-35300869 | None:intergenic |
| GTGTCGTGAGATGAAAATAA+AGG | 0.443191 | 7.2:+35300511 | MS.gene77349:CDS |
| ATGAGCTCAGTGATGAATTT+GGG | 0.445759 | 7.2:+35300432 | MS.gene77349:intron |
| CTAATTTGTGCCTCGTTGAT+CGG | 0.468483 | 7.2:-35300307 | None:intergenic |
| TCAAGAGAAACCATATCATA+AGG | 0.483799 | 7.2:-35300815 | None:intergenic |
| ATGATATGGTTTCTCTTGAT+GGG | 0.487292 | 7.2:+35300819 | MS.gene77349:CDS |
| CAATTCAGAAGAGAGATGAT+TGG | 0.494653 | 7.2:+35300552 | MS.gene77349:CDS |
| GAATCTTCTCCAAATTCAGC+TGG | 0.530268 | 7.2:-35300791 | None:intergenic |
| TAATCCGAGTCTTATGTGAC+AGG | 0.539331 | 7.2:-35300267 | None:intergenic |
| CAAGGCCACTCTACATCTTG+AGG | 0.564026 | 7.2:-35300875 | None:intergenic |
| AATTGCAGACCACATATCAA+TGG | 0.566695 | 7.2:-35300534 | None:intergenic |
| ATTAACTTCCAATATAGACT+TGG | 0.567442 | 7.2:-35300767 | None:intergenic |
| TGTCATAAAAGAAGCAGAGT+GGG | 0.567830 | 7.2:+35300742 | MS.gene77349:CDS |
| AAATAAAGGCCATTGATATG+TGG | 0.571676 | 7.2:+35300525 | MS.gene77349:CDS |
| GGAGTTCATCTATCAACTGA+GGG | 0.572805 | 7.2:+35300689 | MS.gene77349:CDS |
| ATTTGTGCCTCGTTGATCGG+AGG | 0.599779 | 7.2:-35300304 | None:intergenic |
| ATCTTGAGGAAAAGGCACAT+TGG | 0.602253 | 7.2:-35300861 | None:intergenic |
| GGGGAGTGAAATATTAACAA+AGG | 0.628715 | 7.2:+35300709 | MS.gene77349:CDS |
| AGAGCCTGTCACATAAGACT+CGG | 0.665508 | 7.2:+35300263 | None:intergenic |
| ATGTCATAAAAGAAGCAGAG+TGG | 0.667998 | 7.2:+35300741 | MS.gene77349:CDS |
| TGGAGTTCATCTATCAACTG+AGG | 0.669042 | 7.2:+35300688 | MS.gene77349:CDS |
| GAGTTCATCTATCAACTGAG+GGG | 0.728386 | 7.2:+35300690 | MS.gene77349:CDS |
| CAGTAGTCCTCCGATCAACG+AGG | 0.731081 | 7.2:+35300297 | MS.gene77349:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAGATTATACATTTTTTAA+GGG | - | chr7.2:35300357-35300376 | None:intergenic | 10.0% |
| !!! | AATTTAAAACATTTTAGTTT+AGG | - | chr7.2:35300620-35300639 | None:intergenic | 10.0% |
| !! | AGATATATCATAGAATAATT+AGG | - | chr7.2:35300393-35300412 | None:intergenic | 15.0% |
| !! | TCAATAATAATTTAATTACG+TGG | + | chr7.2:35300646-35300665 | MS.gene77349:intron | 15.0% |
| !!! | GAAAGATTATACATTTTTTA+AGG | - | chr7.2:35300358-35300377 | None:intergenic | 15.0% |
| ! | ATGTTTGTTTCATGTAAGTA+TGG | + | chr7.2:35300579-35300598 | MS.gene77349:intron | 25.0% |
| ! | ATTAACTTCCAATATAGACT+TGG | - | chr7.2:35300770-35300789 | None:intergenic | 25.0% |
| !! | GTTTGATTATTTCTTAGTGA+TGG | + | chr7.2:35300668-35300687 | MS.gene77349:intron | 25.0% |
| !!! | TACATTTTTTAAGGGAACAA+AGG | - | chr7.2:35300349-35300368 | None:intergenic | 25.0% |
| AAATAAAGGCCATTGATATG+TGG | + | chr7.2:35300525-35300544 | MS.gene77349:CDS | 30.0% | |
| AGAAGATTCACCTTATGATA+TGG | + | chr7.2:35300805-35300824 | MS.gene77349:CDS | 30.0% | |
| ATGATATGGTTTCTCTTGAT+GGG | + | chr7.2:35300819-35300838 | MS.gene77349:CDS | 30.0% | |
| TATGATATGGTTTCTCTTGA+TGG | + | chr7.2:35300818-35300837 | MS.gene77349:CDS | 30.0% | |
| TCAAGAGAAACCATATCATA+AGG | - | chr7.2:35300818-35300837 | None:intergenic | 30.0% | |
| ! | AATATATTCAGAAGCGTGTT+TGG | + | chr7.2:35300484-35300503 | MS.gene77349:CDS | 30.0% |
| AAGTTAATGCCAGCTGAATT+TGG | + | chr7.2:35300782-35300801 | MS.gene77349:CDS | 35.0% | |
| AATGAGCTCAGTGATGAATT+TGG | + | chr7.2:35300431-35300450 | MS.gene77349:intron | 35.0% | |
| AATTGCAGACCACATATCAA+TGG | - | chr7.2:35300537-35300556 | None:intergenic | 35.0% | |
| ATGAGCTCAGTGATGAATTT+GGG | + | chr7.2:35300432-35300451 | MS.gene77349:intron | 35.0% | |
| ATGTCATAAAAGAAGCAGAG+TGG | + | chr7.2:35300741-35300760 | MS.gene77349:CDS | 35.0% | |
| CAATTCAGAAGAGAGATGAT+TGG | + | chr7.2:35300552-35300571 | MS.gene77349:CDS | 35.0% | |
| GGGGAGTGAAATATTAACAA+AGG | + | chr7.2:35300709-35300728 | MS.gene77349:CDS | 35.0% | |
| GTGTCGTGAGATGAAAATAA+AGG | + | chr7.2:35300511-35300530 | MS.gene77349:CDS | 35.0% | |
| TGTCATAAAAGAAGCAGAGT+GGG | + | chr7.2:35300742-35300761 | MS.gene77349:CDS | 35.0% | |
| AGTGGGAACCAAGTCTATAT+TGG | + | chr7.2:35300759-35300778 | MS.gene77349:CDS | 40.0% | |
| CACTCTACATCTTGAGGAAA+AGG | - | chr7.2:35300872-35300891 | None:intergenic | 40.0% | |
| GAATCTTCTCCAAATTCAGC+TGG | - | chr7.2:35300794-35300813 | None:intergenic | 40.0% | |
| GAGTTCATCTATCAACTGAG+GGG | + | chr7.2:35300690-35300709 | MS.gene77349:CDS | 40.0% | |
| GGAGTTCATCTATCAACTGA+GGG | + | chr7.2:35300689-35300708 | MS.gene77349:CDS | 40.0% | |
| TGGAGTTCATCTATCAACTG+AGG | + | chr7.2:35300688-35300707 | MS.gene77349:CDS | 40.0% | |
| ! | ATCTTGAGGAAAAGGCACAT+TGG | - | chr7.2:35300864-35300883 | None:intergenic | 40.0% |
| ! | CTAATTTGTGCCTCGTTGAT+CGG | - | chr7.2:35300310-35300329 | None:intergenic | 40.0% |
| ! | CTTTTCCTCAAGATGTAGAG+TGG | + | chr7.2:35300870-35300889 | MS.gene77349:CDS | 40.0% |
| ! | TAATCCGAGTCTTATGTGAC+AGG | - | chr7.2:35300270-35300289 | None:intergenic | 40.0% |
| CGATCAACGAGGCACAAATT+AGG | + | chr7.2:35300308-35300327 | MS.gene77349:CDS | 45.0% | |
| ATTTGTGCCTCGTTGATCGG+AGG | - | chr7.2:35300307-35300326 | None:intergenic | 50.0% | |
| CAAGGCCACTCTACATCTTG+AGG | - | chr7.2:35300878-35300897 | None:intergenic | 50.0% | |
| CAGTAGTCCTCCGATCAACG+AGG | + | chr7.2:35300297-35300316 | MS.gene77349:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 35300265 | 35300898 | 35300265 | ID=MS.gene77349 |
| chr7.2 | mRNA | 35300265 | 35300898 | 35300265 | ID=MS.gene77349.t1;Parent=MS.gene77349 |
| chr7.2 | exon | 35300265 | 35300338 | 35300265 | ID=MS.gene77349.t1.exon1;Parent=MS.gene77349.t1 |
| chr7.2 | CDS | 35300265 | 35300338 | 35300265 | ID=cds.MS.gene77349.t1;Parent=MS.gene77349.t1 |
| chr7.2 | exon | 35300442 | 35300591 | 35300442 | ID=MS.gene77349.t1.exon2;Parent=MS.gene77349.t1 |
| chr7.2 | CDS | 35300442 | 35300591 | 35300442 | ID=cds.MS.gene77349.t1;Parent=MS.gene77349.t1 |
| chr7.2 | exon | 35300685 | 35300898 | 35300685 | ID=MS.gene77349.t1.exon3;Parent=MS.gene77349.t1 |
| chr7.2 | CDS | 35300685 | 35300898 | 35300685 | ID=cds.MS.gene77349.t1;Parent=MS.gene77349.t1 |
| Gene Sequence |
| Protein sequence |
