AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83126


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83126.t1 MTR_1g030860 86.875 160 20 1 1 159 77 236 2.09e-81 240
MS.gene83126.t1 MTR_8g077030 35.897 156 93 2 7 159 374 525 1.03e-14 70.9
MS.gene83126.t1 MTR_6g012760 34.615 156 93 3 7 159 106 255 6.83e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83126.t1 AT2G43630 34.356 163 93 2 7 159 104 262 1.00e-24 96.7

Find 42 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATGCAATGGACAAATTTA+AGG 0.142467 1.2:-16254558 None:intergenic
TAAGCGTATCATGGTAAAAT+TGG 0.154842 1.2:-16253672 MS.gene83126:CDS
ATCTGGTGCCTTACTCTTTC+TGG 0.178984 1.2:-16254354 MS.gene83126:intron
CCAGGAGGAGGAGGAGAAGG+TGG 0.330061 1.2:-16254455 MS.gene83126:CDS
CCATGTTGGGGTATTCAAAA+TGG 0.340619 1.2:+16253589 None:intergenic
CAGGATCATACCACCATGTT+GGG 0.367552 1.2:+16253576 None:intergenic
CACCAGATGCGAAAATTGTT+TGG 0.372513 1.2:+16254369 None:intergenic
AATGCAATGGACAAATTTAA+GGG 0.386519 1.2:-16254557 None:intergenic
CCACCTTCTCCTCCTCCTCC+TGG 0.389125 1.2:+16254455 None:intergenic
TTACGGAAGCAAATTCAAAA+AGG 0.403502 1.2:-16254515 MS.gene83126:CDS
TTAAGGTTTATAGTTGGCTT+AGG 0.406725 1.2:-16253710 MS.gene83126:CDS
AGGAGGAGGAGGAGAAGGTG+GGG 0.423587 1.2:-16254453 MS.gene83126:CDS
GGTGGTGGTGGCAGTCCCAA+TGG 0.428342 1.2:-16254431 MS.gene83126:CDS
GGTGGCAGTCCCAATGGCCC+TGG 0.434107 1.2:-16254425 MS.gene83126:CDS
AAGCGTATCATGGTAAAATT+GGG 0.456666 1.2:-16253671 MS.gene83126:CDS
GGAGGAGGAGGAGAAGGTGG+GGG 0.460530 1.2:-16254452 MS.gene83126:CDS
TATTTAGACAATGGTGGAAG+TGG 0.463016 1.2:-16254488 MS.gene83126:CDS
CCACCAGGAGGAGGAGGAGA+AGG 0.467905 1.2:-16254458 MS.gene83126:CDS
AATCAATAGAAGAAGTATTA+CGG 0.472780 1.2:-16254532 MS.gene83126:CDS
TCAGATCCACCAGGGCCATT+GGG 0.486936 1.2:+16254416 None:intergenic
ATCAGATCCACCAGGGCCAT+TGG 0.489944 1.2:+16254415 None:intergenic
AGAGTGTACCAGAAAGAGTA+AGG 0.496063 1.2:+16254346 None:intergenic
GTAAAGCCACCAGGAGGAGG+AGG 0.507231 1.2:-16254464 MS.gene83126:CDS
CAGGAGGAGGAGGAGAAGGT+GGG 0.514543 1.2:-16254454 MS.gene83126:CDS
GGAGTAAAGCCACCAGGAGG+AGG 0.524624 1.2:-16254467 MS.gene83126:CDS
TCAGGATCATACCACCATGT+TGG 0.534231 1.2:+16253575 None:intergenic
GGCAGTCCCAATGGCCCTGG+TGG 0.545700 1.2:-16254422 MS.gene83126:CDS
TAGTCGAATTAAGCGTATCA+TGG 0.558198 1.2:-16253681 MS.gene83126:CDS
TAAAATTGGGAAAATTGTAC+CGG 0.563003 1.2:-16253658 MS.gene83126:CDS
GGTGAGTATTTAGACAATGG+TGG 0.570123 1.2:-16254494 MS.gene83126:CDS
ACTGCTGTACGAAAATCCTC+AGG 0.570351 1.2:+16253557 None:intergenic
CCTTCTCCTCCTCCTCCTGG+TGG 0.583094 1.2:+16254458 None:intergenic
TCTTCATCATCAGATCCACC+AGG 0.590752 1.2:+16254407 None:intergenic
AAAGGTGAGTATTTAGACAA+TGG 0.597947 1.2:-16254497 MS.gene83126:CDS
TATCTATCTCATCATGAGCA+AGG 0.607713 1.2:-16253759 MS.gene83126:CDS
AACATGGTGGTATGATCCTG+AGG 0.623546 1.2:-16253573 MS.gene83126:CDS
GGAAGTGGAGTAAAGCCACC+AGG 0.635749 1.2:-16254473 MS.gene83126:CDS
AGGATCATACCACCATGTTG+GGG 0.658146 1.2:+16253577 None:intergenic
TGTACCGGAGTACGAAACAG+AGG 0.680091 1.2:-16253643 MS.gene83126:CDS
GAATTACTATGCATCAGATG+CGG 0.684768 1.2:-16253531 MS.gene83126:CDS
CTTCATCATCAGATCCACCA+GGG 0.689052 1.2:+16254408 None:intergenic
AGTGGAGTAAAGCCACCAGG+AGG 0.725114 1.2:-16254470 MS.gene83126:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAATTTTGATGTCAAATT+TGG - chr1.2:16254123-16254142 MS.gene83126:intron 15.0%
!!! AATCAATAGAAGAAGTATTA+CGG - chr1.2:16253542-16253561 MS.gene83126:CDS 20.0%
!!! TAGTTAATGTTTCTGATTAA+TGG - chr1.2:16253889-16253908 MS.gene83126:intron 20.0%
!!! TCTAAATTTTGAAAACTCAA+AGG - chr1.2:16253828-16253847 MS.gene83126:intron 20.0%
! ATAATTTCTCTTCAGAAATG+AGG + chr1.2:16254093-16254112 None:intergenic 25.0%
! TAAAATTGGGAAAATTGTAC+CGG - chr1.2:16254416-16254435 MS.gene83126:CDS 25.0%
!!! GACTTTTTAAGGTTTATAGT+TGG - chr1.2:16254358-16254377 MS.gene83126:CDS 25.0%
!!! GCACAATTTTTGACTTTTTA+AGG - chr1.2:16254347-16254366 MS.gene83126:intron 25.0%
!!! TAACAGAGTTGATAGAATAT+AGG - chr1.2:16254062-16254081 MS.gene83126:intron 25.0%
AAAGGTGAGTATTTAGACAA+TGG - chr1.2:16253577-16253596 MS.gene83126:CDS 30.0%
AACTCTGTTAATCATGTCAA+GGG + chr1.2:16254052-16254071 None:intergenic 30.0%
AAGCGTATCATGGTAAAATT+GGG - chr1.2:16254403-16254422 MS.gene83126:CDS 30.0%
ACTCAAAGGAGAAAGTTAAA+TGG - chr1.2:16253842-16253861 MS.gene83126:intron 30.0%
GTTTGAGCATTAAGAAATAG+TGG + chr1.2:16254275-16254294 None:intergenic 30.0%
TAAGCGTATCATGGTAAAAT+TGG - chr1.2:16254402-16254421 MS.gene83126:CDS 30.0%
TCAAAAGTCTTCTCATATAC+CGG + chr1.2:16253989-16254008 None:intergenic 30.0%
TCATGATGAGATAGATATAC+TGG + chr1.2:16254312-16254331 None:intergenic 30.0%
TTACGGAAGCAAATTCAAAA+AGG - chr1.2:16253559-16253578 MS.gene83126:CDS 30.0%
! TTAAGGTTTATAGTTGGCTT+AGG - chr1.2:16254364-16254383 MS.gene83126:CDS 30.0%
CAACTCTGTTAATCATGTCA+AGG + chr1.2:16254053-16254072 None:intergenic 35.0%
CGGTATATGTGTAGTATATG+TGG - chr1.2:16253760-16253779 MS.gene83126:CDS 35.0%
GAATTACTATGCATCAGATG+CGG - chr1.2:16254543-16254562 MS.gene83126:CDS 35.0%
GGACAAGTAAATTACTCATC+TGG - chr1.2:16253781-16253800 MS.gene83126:intron 35.0%
TAGTCGAATTAAGCGTATCA+TGG - chr1.2:16254393-16254412 MS.gene83126:CDS 35.0%
TATCTATCTCATCATGAGCA+AGG - chr1.2:16254315-16254334 MS.gene83126:intron 35.0%
TTCTCTTCAGAAATGAGGAA+TGG + chr1.2:16254088-16254107 None:intergenic 35.0%
! AATCACTGATGAGCACTTTT+TGG - chr1.2:16254456-16254475 MS.gene83126:CDS 35.0%
! TATGAGAAGACTTTTGAGCA+TGG - chr1.2:16253992-16254011 MS.gene83126:intron 35.0%
! TATTTAGACAATGGTGGAAG+TGG - chr1.2:16253586-16253605 MS.gene83126:CDS 35.0%
!! AACTATAGCCCTTTTTAGCA+TGG + chr1.2:16253874-16253893 None:intergenic 35.0%
AAGTCTTCTCATATACCGGA+AGG + chr1.2:16253985-16254004 None:intergenic 40.0%
AGAGTGTACCAGAAAGAGTA+AGG + chr1.2:16253731-16253750 None:intergenic 40.0%
CACCAGATGCGAAAATTGTT+TGG + chr1.2:16253708-16253727 None:intergenic 40.0%
GCTAACTATTGAAGTCCTTC+CGG - chr1.2:16253967-16253986 MS.gene83126:intron 40.0%
GGAAGTAGCCATGCTAAAAA+GGG - chr1.2:16253863-16253882 MS.gene83126:intron 40.0%
GGTGAGTATTTAGACAATGG+TGG - chr1.2:16253580-16253599 MS.gene83126:CDS 40.0%
TGGAAGTAGCCATGCTAAAA+AGG - chr1.2:16253862-16253881 MS.gene83126:intron 40.0%
TGGTACACTCTCTTTCTCTT+CGG - chr1.2:16253740-16253759 MS.gene83126:CDS 40.0%
! CCATGTTGGGGTATTCAAAA+TGG + chr1.2:16254488-16254507 None:intergenic 40.0%
! CCATTTTGAATACCCCAACA+TGG - chr1.2:16254485-16254504 MS.gene83126:CDS 40.0%
! TTTTCCTCTGTTTCGTACTC+CGG + chr1.2:16254438-16254457 None:intergenic 40.0%
! TTTTGAATACCCCAACATGG+TGG - chr1.2:16254488-16254507 MS.gene83126:CDS 40.0%
!! TGATGAGCACTTTTTGGAGA+AGG - chr1.2:16254462-16254481 MS.gene83126:CDS 40.0%
AACATGGTGGTATGATCCTG+AGG - chr1.2:16254501-16254520 MS.gene83126:CDS 45.0%
ACTGCTGTACGAAAATCCTC+AGG + chr1.2:16254520-16254539 None:intergenic 45.0%
AGGATCATACCACCATGTTG+GGG + chr1.2:16254500-16254519 None:intergenic 45.0%
ATCTGGTGCCTTACTCTTTC+TGG - chr1.2:16253720-16253739 MS.gene83126:CDS 45.0%
CAGGATCATACCACCATGTT+GGG + chr1.2:16254501-16254520 None:intergenic 45.0%
CTTCATCATCAGATCCACCA+GGG + chr1.2:16253669-16253688 None:intergenic 45.0%
TCAGGATCATACCACCATGT+TGG + chr1.2:16254502-16254521 None:intergenic 45.0%
TCTTCATCATCAGATCCACC+AGG + chr1.2:16253670-16253689 None:intergenic 45.0%
! CGCCAAACAATTTTCGCATC+TGG - chr1.2:16253703-16253722 MS.gene83126:CDS 45.0%
TGTACCGGAGTACGAAACAG+AGG - chr1.2:16254431-16254450 MS.gene83126:CDS 50.0%
AGTGGAGTAAAGCCACCAGG+AGG - chr1.2:16253604-16253623 MS.gene83126:CDS 55.0%
ATCAGATCCACCAGGGCCAT+TGG + chr1.2:16253662-16253681 None:intergenic 55.0%
GGAAGTGGAGTAAAGCCACC+AGG - chr1.2:16253601-16253620 MS.gene83126:CDS 55.0%
TCAGATCCACCAGGGCCATT+GGG + chr1.2:16253661-16253680 None:intergenic 55.0%
AGGAGGAGGAGGAGAAGGTG+GGG - chr1.2:16253621-16253640 MS.gene83126:CDS 60.0%
CAGGAGGAGGAGGAGAAGGT+GGG - chr1.2:16253620-16253639 MS.gene83126:CDS 60.0%
GGAGTAAAGCCACCAGGAGG+AGG - chr1.2:16253607-16253626 MS.gene83126:CDS 60.0%
GTAAAGCCACCAGGAGGAGG+AGG - chr1.2:16253610-16253629 MS.gene83126:CDS 60.0%
CCACCAGGAGGAGGAGGAGA+AGG - chr1.2:16253616-16253635 MS.gene83126:CDS 65.0%
CCACCTTCTCCTCCTCCTCC+TGG + chr1.2:16253622-16253641 None:intergenic 65.0%
CCAGGAGGAGGAGGAGAAGG+TGG - chr1.2:16253619-16253638 MS.gene83126:CDS 65.0%
CCTTCTCCTCCTCCTCCTGG+TGG + chr1.2:16253619-16253638 None:intergenic 65.0%
GGAGGAGGAGGAGAAGGTGG+GGG - chr1.2:16253622-16253641 MS.gene83126:CDS 65.0%
!! GGTGGTGGTGGCAGTCCCAA+TGG - chr1.2:16253643-16253662 MS.gene83126:CDS 65.0%
GGAGGAGAAGGTGGGGGTGG+TGG - chr1.2:16253628-16253647 MS.gene83126:CDS 70.0%
GGAGGAGGAGAAGGTGGGGG+TGG - chr1.2:16253625-16253644 MS.gene83126:CDS 70.0%
GGCAGTCCCAATGGCCCTGG+TGG - chr1.2:16253652-16253671 MS.gene83126:CDS 70.0%
GGTGGCAGTCCCAATGGCCC+TGG - chr1.2:16253649-16253668 MS.gene83126:CDS 70.0%
! GGAGAAGGTGGGGGTGGTGG+TGG - chr1.2:16253631-16253650 MS.gene83126:CDS 70.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 16253523 16254573 16253523 ID=MS.gene83126
chr1.2 mRNA 16253523 16254573 16253523 ID=MS.gene83126.t1;Parent=MS.gene83126
chr1.2 exon 16254355 16254573 16254355 ID=MS.gene83126.t1.exon1;Parent=MS.gene83126.t1
chr1.2 CDS 16254355 16254573 16254355 ID=cds.MS.gene83126.t1;Parent=MS.gene83126.t1
chr1.2 exon 16253523 16253783 16253523 ID=MS.gene83126.t1.exon2;Parent=MS.gene83126.t1
chr1.2 CDS 16253523 16253783 16253523 ID=cds.MS.gene83126.t1;Parent=MS.gene83126.t1
Gene Sequence

>MS.gene83126

ATGGACAAATTTAAGGGACAATCAATAGAAGAAGTATTACGGAAGCAAATTCAAAAAGGTGAGTATTTAGACAATGGTGGAAGTGGAGTAAAGCCACCAGGAGGAGGAGGAGAAGGTGGGGGTGGTGGTGGCAGTCCCAATGGCCCTGGTGGATCTGATGATGAAGAAAGCTATGACACTCGCCAAACAATTTTCGCATCTGGTGCCTTACTCTTTCTGGTACACTCTCTTTCTCTTCGGTATATGTGTAGTATATGTGGACAAGTAAATTACTCATCTGGCATTCTTTATTTTGTGTGCAAAACTCTAAATTTTGAAAACTCAAAGGAGAAAGTTAAATGGAAGTAGCCATGCTAAAAAGGGCTATAGTTAATGTTTCTGATTAATGGAACAAAACTTTCGAATTAAACTGCATTAATAGAATTTAAATCACTGATAGTTGTAGCTAACTATTGAAGTCCTTCCGGTATATGAGAAGACTTTTGAGCATGGAATATGTTCTTATGCAAACTTATGATACGAGTTACCCTTGACATGATTAACAGAGTTGATAGAATATAGGCCATTCCTCATTTCTGAAGAGAAATTATTTTTATTCATAAAAATTTTGATGTCAAATTTGGTGTCTTTTGATTTTTGTGTGTATTATTCAAATATGCGTCTATTATAGAAAGATAAAAAGTAAGAGAGACATAGAAATGAATGCTTACAATGCAGTATATAGTGTATGAATTTTGATGTTCAAATAACCACTATTTCTTAATGCTCAAACTCTACATTATTTTTCCAGTATATCTATCTCATCATGAGCAAGGAGCTTCTAAGCACAATTTTTGACTTTTTAAGGTTTATAGTTGGCTTAGGTCAGACTAGTCGAATTAAGCGTATCATGGTAAAATTGGGAAAATTGTACCGGAGTACGAAACAGAGGAAAATCACTGATGAGCACTTTTTGGAGAAGGCCATTTTGAATACCCCAACATGGTGGTATGATCCTGAGGATTTTCGTACAGCAGTTAAGAATTACTATGCATCAGATGCGGACGAATAA

Protein sequence

>MS.gene83126.t1

MDKFKGQSIEEVLRKQIQKGEYLDNGGSGVKPPGGGGEGGGGGGSPNGPGGSDDEESYDTRQTIFASGALLFLYIYLIMSKELLSTIFDFLRFIVGLGQTSRIKRIMVKLGKLYRSTKQRKITDEHFLEKAILNTPTWWYDPEDFRTAVKNYYASDADE