Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180006218.01.T01 | KEH44538.1 | 76.847 | 203 | 24 | 4 | 1 | 203 | 1 | 180 | 9.60E-98 | 298 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180006218.01.T01 | Q9MAT6 | 44.706 | 170 | 63 | 7 | 1 | 170 | 1 | 139 | 7.99E-32 | 123 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180006218.01.T01 | A0A072VSX7 | 76.847 | 203 | 24 | 4 | 1 | 203 | 1 | 180 | 4.59e-98 | 298 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0180006218.01.T01 | TR | ARID |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048068.01 | MsG0180006218.01 | 0.800455 | 1.429579e-48 | 6.659283e-46 |
| MsG0080048777.01 | MsG0180006218.01 | 0.800867 | 1.178203e-48 | 5.546150e-46 |
| MsG0080048780.01 | MsG0180006218.01 | 0.813723 | 2.215095e-51 | 1.457817e-48 |
| MsG0080048801.01 | MsG0180006218.01 | 0.830794 | 2.402176e-55 | 2.557582e-52 |
| MsG0080048845.01 | MsG0180006218.01 | 0.803172 | 3.957575e-49 | 1.975248e-46 |
| MsG0080049029.01 | MsG0180006218.01 | 0.856490 | 3.105072e-62 | 7.425108e-59 |
| MsG0080049039.01 | MsG0180006218.01 | 0.810362 | 1.196828e-50 | 7.199875e-48 |
| MsG0080049040.01 | MsG0180006218.01 | 0.849152 | 3.884067e-60 | 7.296929e-57 |
| MsG0080049054.01 | MsG0180006218.01 | 0.804948 | 1.690559e-49 | 8.832562e-47 |
| MsG0080049068.01 | MsG0180006218.01 | 0.819704 | 1.010014e-52 | 7.830309e-50 |
| MsG0180000246.01 | MsG0180006218.01 | 0.803263 | 3.788882e-49 | 1.895452e-46 |
| MsG0180000638.01 | MsG0180006218.01 | 0.800767 | 1.234926e-48 | 5.798170e-46 |
| MsG0180000670.01 | MsG0180006218.01 | 0.826915 | 2.087472e-54 | 1.984782e-51 |
| MsG0180000671.01 | MsG0180006218.01 | 0.817850 | 2.663055e-52 | 1.961422e-49 |
| MsG0180000677.01 | MsG0180006218.01 | 0.824453 | 8.005169e-54 | 7.093344e-51 |
| MsG0180000792.01 | MsG0180006218.01 | 0.819843 | 9.389202e-53 | 7.307503e-50 |
| MsG0180000843.01 | MsG0180006218.01 | 0.801817 | 7.527233e-49 | 3.629438e-46 |
| MsG0180000989.01 | MsG0180006218.01 | 0.839896 | 1.204284e-57 | 1.686748e-54 |
| MsG0180001085.01 | MsG0180006218.01 | 0.857958 | 1.144708e-62 | 2.875017e-59 |
| MsG0180001175.01 | MsG0180006218.01 | 0.833533 | 5.050972e-56 | 5.833301e-53 |
| MsG0180001270.01 | MsG0180006218.01 | 0.831376 | 1.729041e-55 | 1.872892e-52 |
| MsG0180001371.01 | MsG0180006218.01 | 0.801958 | 7.041266e-49 | 3.407183e-46 |
| MsG0180001929.01 | MsG0180006218.01 | 0.822892 | 1.857664e-53 | 1.575057e-50 |
| MsG0180002671.01 | MsG0180006218.01 | 0.802577 | 5.252572e-49 | 2.582020e-46 |
| MsG0180003316.01 | MsG0180006218.01 | 0.808091 | 3.672368e-50 | 2.080701e-47 |
| MsG0180003325.01 | MsG0180006218.01 | 0.803077 | 4.140953e-49 | 2.061609e-46 |
| MsG0180003531.01 | MsG0180006218.01 | 0.820331 | 7.257135e-53 | 5.725035e-50 |
| MsG0180004103.01 | MsG0180006218.01 | 0.813535 | 2.436449e-51 | 1.595371e-48 |
| MsG0180004448.01 | MsG0180006218.01 | 0.837281 | 5.701759e-57 | 7.370375e-54 |
| MsG0180004518.01 | MsG0180006218.01 | 0.812058 | 5.130748e-51 | 3.228754e-48 |
| MsG0180004733.01 | MsG0180006218.01 | 0.837186 | 6.028917e-57 | 7.771022e-54 |
| MsG0180004735.01 | MsG0180006218.01 | 0.822804 | 1.947618e-53 | 1.647188e-50 |
| MsG0180004934.01 | MsG0180006218.01 | 0.816034 | 6.806844e-52 | 4.770425e-49 |
| MsG0180005276.01 | MsG0180006218.01 | 0.823999 | 1.023262e-53 | 8.950651e-51 |
| MsG0180005349.01 | MsG0180006218.01 | 0.832646 | 8.396135e-56 | 9.442888e-53 |
| MsG0180005418.01 | MsG0180006218.01 | 0.814869 | 1.236823e-51 | 8.395610e-49 |
| MsG0180005492.01 | MsG0180006218.01 | 0.841563 | 4.405591e-58 | 6.498867e-55 |
| MsG0180005961.01 | MsG0180006218.01 | 0.804130 | 2.503520e-49 | 1.280640e-46 |
| MsG0180006136.01 | MsG0180006218.01 | 0.846557 | 2.015573e-59 | 3.482633e-56 |
| MsG0180006176.01 | MsG0180006218.01 | 0.802158 | 6.406428e-49 | 3.115885e-46 |
| MsG0180006200.01 | MsG0180006218.01 | 0.809041 | 2.301645e-50 | 1.337095e-47 |
| MsG0180006218.01 | MsG0280006718.01 | 0.814290 | 1.661396e-51 | 1.110339e-48 |
| MsG0180006218.01 | MsG0280006805.01 | 0.808926 | 2.436161e-50 | 1.410835e-47 |
| MsG0180006218.01 | MsG0280007502.01 | 0.820750 | 5.816665e-53 | 4.642392e-50 |
| MsG0180006218.01 | MsG0280007814.01 | 0.803145 | 4.007886e-49 | 1.998920e-46 |
| MsG0180006218.01 | MsG0280007831.01 | 0.826369 | 2.817123e-54 | 2.636904e-51 |
| MsG0180006218.01 | MsG0280007852.01 | 0.802430 | 5.631732e-49 | 2.758223e-46 |
| MsG0180006218.01 | MsG0280008223.01 | 0.845139 | 4.893378e-59 | 8.076164e-56 |
| MsG0180006218.01 | MsG0280008388.01 | 0.807239 | 5.570298e-50 | 3.086603e-47 |
| MsG0180006218.01 | MsG0280008451.01 | 0.803651 | 3.149620e-49 | 1.591372e-46 |
| MsG0180006218.01 | MsG0280008591.01 | 0.804088 | 2.554636e-49 | 1.305414e-46 |
| MsG0180006218.01 | MsG0280008922.01 | 0.862724 | 4.138531e-64 | 1.224251e-60 |
| MsG0180006218.01 | MsG0280008942.01 | 0.800359 | 1.495721e-48 | 6.950709e-46 |
| MsG0180006218.01 | MsG0280009756.01 | 0.815761 | 7.832275e-52 | 5.448339e-49 |
| MsG0180006218.01 | MsG0280009853.01 | 0.803285 | 3.749436e-49 | 1.876736e-46 |
| MsG0180006218.01 | MsG0280010353.01 | 0.838949 | 2.121530e-57 | 2.886102e-54 |
| MsG0180006218.01 | MsG0280010376.01 | 0.837040 | 6.569931e-57 | 8.431153e-54 |
| MsG0180006218.01 | MsG0280010926.01 | 0.806804 | 6.884406e-50 | 3.771944e-47 |
| MsG0180006218.01 | MsG0280011411.01 | 0.816195 | 6.267161e-52 | 4.411529e-49 |
| MsG0180006218.01 | MsG0380013472.01 | 0.840190 | 1.009621e-57 | 1.427177e-54 |
| MsG0180006218.01 | MsG0380014588.01 | 0.826745 | 2.292124e-54 | 2.168430e-51 |
| MsG0180006218.01 | MsG0380015356.01 | 0.815079 | 1.110944e-51 | 7.584190e-49 |
| MsG0180006218.01 | MsG0380015547.01 | 0.845269 | 4.511933e-59 | 7.478790e-56 |
| MsG0180006218.01 | MsG0380015603.01 | 0.824003 | 1.021380e-53 | 8.935083e-51 |
| MsG0180006218.01 | MsG0380016057.01 | 0.829211 | 5.843964e-55 | 5.941948e-52 |
| MsG0180006218.01 | MsG0380016058.01 | 0.840380 | 9.003163e-58 | 1.280187e-54 |
| MsG0180006218.01 | MsG0380016194.01 | 0.822639 | 2.127358e-53 | 1.790934e-50 |
| MsG0180006218.01 | MsG0380016284.01 | 0.815731 | 7.955374e-52 | 5.529477e-49 |
| MsG0180006218.01 | MsG0380017703.01 | 0.818096 | 2.342350e-52 | 1.736765e-49 |
| MsG0180006218.01 | MsG0380017728.01 | 0.809900 | 1.505380e-50 | 8.947293e-48 |
| MsG0180006218.01 | MsG0380018061.01 | 0.830740 | 2.477600e-55 | 2.633626e-52 |
| MsG0180006218.01 | MsG0380018069.01 | 0.846402 | 2.222076e-59 | 3.820305e-56 |
| MsG0180006218.01 | MsG0480018107.01 | 0.813915 | 2.009511e-51 | 1.329367e-48 |
| MsG0180006218.01 | MsG0480018592.01 | 0.836153 | 1.105444e-56 | 1.381277e-53 |
| MsG0180006218.01 | MsG0480018644.01 | 0.837632 | 4.634034e-57 | 6.056257e-54 |
| MsG0180006218.01 | MsG0480019501.01 | 0.822166 | 2.740045e-53 | 2.275572e-50 |
| MsG0180006218.01 | MsG0480019751.01 | 0.800174 | 1.631281e-48 | 7.544729e-46 |
| MsG0180006218.01 | MsG0480019956.01 | 0.822265 | 2.599024e-53 | 2.164673e-50 |
| MsG0180006218.01 | MsG0480019971.01 | 0.826942 | 2.055908e-54 | 1.956297e-51 |
| MsG0180006218.01 | MsG0480020151.01 | 0.817194 | 3.741191e-52 | 2.706303e-49 |
| MsG0180006218.01 | MsG0480020350.01 | 0.807134 | 5.863229e-50 | 3.239897e-47 |
| MsG0180006218.01 | MsG0480020690.01 | 0.820910 | 5.344025e-53 | 4.284418e-50 |
| MsG0180006218.01 | MsG0480020753.01 | 0.806501 | 7.978297e-50 | 4.337340e-47 |
| MsG0180006218.01 | MsG0480021168.01 | 0.838109 | 3.495758e-57 | 4.635271e-54 |
| MsG0180006218.01 | MsG0480021248.01 | 0.826193 | 3.101919e-54 | 2.888904e-51 |
| MsG0180006218.01 | MsG0480021294.01 | 0.803094 | 4.108108e-49 | 2.046116e-46 |
| MsG0180006218.01 | MsG0480021783.01 | 0.834676 | 2.611737e-56 | 3.121269e-53 |
| MsG0180006218.01 | MsG0480021803.01 | 0.802053 | 6.733559e-49 | 3.266228e-46 |
| MsG0180006218.01 | MsG0480021817.01 | 0.824483 | 7.876642e-54 | 6.985794e-51 |
| MsG0180006218.01 | MsG0480022130.01 | 0.807420 | 5.100399e-50 | 2.839517e-47 |
| MsG0180006218.01 | MsG0480022149.01 | 0.807845 | 4.142332e-50 | 2.331731e-47 |
| MsG0180006218.01 | MsG0480022260.01 | 0.846681 | 1.864299e-59 | 3.234489e-56 |
| MsG0180006218.01 | MsG0480022646.01 | 0.837650 | 4.587395e-57 | 5.998521e-54 |
| MsG0180006218.01 | MsG0480022647.01 | 0.828551 | 8.444955e-55 | 8.421659e-52 |
| MsG0180006218.01 | MsG0480022994.01 | 0.804893 | 1.735892e-49 | 9.057090e-47 |
| MsG0180006218.01 | MsG0480023216.01 | 0.809979 | 1.447410e-50 | 8.620842e-48 |
| MsG0180006218.01 | MsG0480023314.01 | 0.833215 | 6.062740e-56 | 6.935667e-53 |
| MsG0180006218.01 | MsG0480023351.01 | 0.802427 | 5.639975e-49 | 2.762101e-46 |
| MsG0180006218.01 | MsG0480023367.01 | 0.843279 | 1.545080e-58 | 2.405400e-55 |
| MsG0180006218.01 | MsG0480023389.01 | 0.818643 | 1.760737e-52 | 1.325421e-49 |
| MsG0180006218.01 | MsG0480023444.01 | 0.826534 | 2.573354e-54 | 2.419866e-51 |
| MsG0180006218.01 | MsG0480023845.01 | 0.821051 | 4.959776e-53 | 3.992217e-50 |
| MsG0180006218.01 | MsG0480023989.01 | 0.802186 | 6.321880e-49 | 3.076873e-46 |
| MsG0180006218.01 | MsG0480023990.01 | 0.860891 | 1.505783e-63 | 4.180337e-60 |
| MsG0180006218.01 | MsG0480023991.01 | 0.859027 | 5.491837e-63 | 1.429918e-59 |
| MsG0180006218.01 | MsG0480023992.01 | 0.852732 | 3.805564e-61 | 8.028000e-58 |
| MsG0180006218.01 | MsG0580024285.01 | 0.822354 | 2.477722e-53 | 2.068991e-50 |
| MsG0180006218.01 | MsG0580024679.01 | 0.813616 | 2.339125e-51 | 1.534981e-48 |
| MsG0180006218.01 | MsG0580024873.01 | 0.823521 | 1.324385e-53 | 1.143052e-50 |
| MsG0180006218.01 | MsG0580025502.01 | 0.823076 | 1.682853e-53 | 1.434367e-50 |
| MsG0180006218.01 | MsG0580025906.01 | 0.809513 | 1.822912e-50 | 1.072421e-47 |
| MsG0180006218.01 | MsG0580027082.01 | 0.804543 | 2.053901e-49 | 1.061841e-46 |
| MsG0180006218.01 | MsG0580027394.01 | 0.816950 | 4.245503e-52 | 3.051036e-49 |
| MsG0180006218.01 | MsG0580027979.01 | 0.805172 | 1.517711e-49 | 7.974848e-47 |
| MsG0180006218.01 | MsG0580028024.01 | 0.813203 | 2.881924e-51 | 1.869930e-48 |
| MsG0180006218.01 | MsG0580028033.01 | 0.806303 | 8.782904e-50 | 4.750707e-47 |
| MsG0180006218.01 | MsG0580028306.01 | 0.825841 | 3.762534e-54 | 3.469268e-51 |
| MsG0180006218.01 | MsG0580029605.01 | 0.805641 | 1.210125e-49 | 6.435771e-47 |
| MsG0180006218.01 | MsG0580029862.01 | 0.832520 | 9.019032e-56 | 1.010523e-52 |
| MsG0180006218.01 | MsG0680030464.01 | 0.831454 | 1.653825e-55 | 1.795638e-52 |
| MsG0180006218.01 | MsG0680030514.01 | 0.806407 | 8.350811e-50 | 4.528888e-47 |
| MsG0180006218.01 | MsG0680030516.01 | 0.838961 | 2.106707e-57 | 2.866935e-54 |
| MsG0180006218.01 | MsG0680030627.01 | 0.813873 | 2.053662e-51 | 1.357040e-48 |
| MsG0180006218.01 | MsG0680030858.01 | 0.845453 | 4.024129e-59 | 6.709094e-56 |
| MsG0180006218.01 | MsG0680030957.01 | 0.805320 | 1.413034e-49 | 7.453062e-47 |
| MsG0180006218.01 | MsG0680032006.01 | 0.827756 | 1.312335e-54 | 1.278639e-51 |
| MsG0180006218.01 | MsG0680032057.01 | 0.803873 | 2.832238e-49 | 1.439333e-46 |
| MsG0180006218.01 | MsG0680032238.01 | 0.815711 | 8.035889e-52 | 5.582238e-49 |
| MsG0180006218.01 | MsG0680032850.01 | 0.801027 | 1.092652e-48 | 5.164719e-46 |
| MsG0180006218.01 | MsG0680033377.01 | 0.822963 | 1.788044e-53 | 1.519063e-50 |
| MsG0180006218.01 | MsG0680035731.01 | 0.839694 | 1.359444e-57 | 1.892059e-54 |
| MsG0180006218.01 | MsG0680035791.01 | 0.800812 | 1.209303e-48 | 5.684231e-46 |
| MsG0180006218.01 | MsG0780036792.01 | 0.811102 | 8.280446e-51 | 5.080463e-48 |
| MsG0180006218.01 | MsG0780037184.01 | 0.806283 | 8.869192e-50 | 4.794939e-47 |
| MsG0180006218.01 | MsG0780037363.01 | 0.800417 | 1.455395e-48 | 6.773319e-46 |
| MsG0180006218.01 | MsG0780037662.01 | 0.836719 | 7.936612e-57 | 1.008645e-53 |
| MsG0180006218.01 | MsG0780038056.01 | 0.810221 | 1.284148e-50 | 7.696122e-48 |
| MsG0180006218.01 | MsG0780038616.01 | 0.808141 | 3.582758e-50 | 2.032652e-47 |
| MsG0180006218.01 | MsG0780038784.01 | 0.814402 | 1.569446e-51 | 1.052088e-48 |
| MsG0180006218.01 | MsG0780039161.01 | 0.804565 | 2.032398e-49 | 1.051333e-46 |
| MsG0180006218.01 | MsG0780039675.01 | 0.810105 | 1.359655e-50 | 8.124881e-48 |
| MsG0180006218.01 | MsG0780039746.01 | 0.822190 | 2.704275e-53 | 2.247377e-50 |
| MsG0180006218.01 | MsG0780039853.01 | 0.845276 | 4.492507e-59 | 7.448297e-56 |
| MsG0180006218.01 | MsG0780039988.01 | 0.819909 | 9.068058e-53 | 7.070682e-50 |
| MsG0180006218.01 | MsG0780040484.01 | 0.822419 | 2.392447e-53 | 2.001530e-50 |
| MsG0180006218.01 | MsG0780041138.01 | 0.816768 | 4.664013e-52 | 3.334835e-49 |
| MsG0180006218.01 | MsG0780041174.01 | 0.845902 | 3.039317e-59 | 5.141966e-56 |
| MsG0180006218.01 | MsG0780041302.01 | 0.809300 | 2.025821e-50 | 1.185069e-47 |
| MsG0180006218.01 | MsG0880041931.01 | 0.830094 | 3.564587e-55 | 3.718167e-52 |
| MsG0180006218.01 | MsG0880043277.01 | 0.839706 | 1.349734e-57 | 1.879268e-54 |
| MsG0180006218.01 | MsG0880043278.01 | 0.852142 | 5.606313e-61 | 1.160451e-57 |
| MsG0180006218.01 | MsG0880043290.01 | 0.817117 | 3.894257e-52 | 2.811099e-49 |
| MsG0180006218.01 | MsG0880044392.01 | 0.824212 | 9.122244e-54 | 8.028159e-51 |
| MsG0180006218.01 | MsG0880044414.01 | 0.806812 | 6.856928e-50 | 3.757778e-47 |
| MsG0180006218.01 | MsG0880044487.01 | 0.828381 | 9.281763e-55 | 9.210600e-52 |
| MsG0180006218.01 | MsG0880045101.01 | 0.839578 | 1.456911e-57 | 2.020552e-54 |
| MsG0180006218.01 | MsG0880045361.01 | 0.820670 | 6.067321e-53 | 4.831407e-50 |
| MsG0180006218.01 | MsG0880046054.01 | 0.832209 | 1.076970e-55 | 1.195564e-52 |
| MsG0180006218.01 | MsG0880046267.01 | 0.800610 | 1.329385e-48 | 6.217010e-46 |
| MsG0180006218.01 | MsG0880046382.01 | 0.851665 | 7.652224e-61 | 1.560135e-57 |
| MsG0180006218.01 | MsG0880046764.01 | 0.810436 | 1.153650e-50 | 6.953431e-48 |
| MsG0180006218.01 | MsG0880046765.01 | 0.830154 | 3.445529e-55 | 3.600570e-52 |
| MsG0180006218.01 | MsG0880046791.01 | 0.810349 | 1.204951e-50 | 7.246147e-48 |
| MsG0180006218.01 | MsG0880047451.01 | 0.812103 | 5.016864e-51 | 3.161082e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180006218.01.T01 | MTR_1g116180 | 76.847 | 203 | 24 | 4 | 1 | 203 | 1 | 180 | 1.16e-101 | 298 |
| MsG0180006218.01.T01 | MTR_1g116180 | 76.847 | 203 | 24 | 4 | 1 | 203 | 1 | 180 | 1.81e-101 | 300 |
| MsG0180006218.01.T01 | MTR_7g005970 | 52.000 | 200 | 70 | 8 | 1 | 198 | 1 | 176 | 1.52e-55 | 182 |
| MsG0180006218.01.T01 | MTR_3g114920 | 40.123 | 162 | 73 | 5 | 24 | 185 | 20 | 157 | 8.99e-25 | 99.8 |
| MsG0180006218.01.T01 | MTR_3g009890 | 41.732 | 127 | 51 | 4 | 24 | 150 | 29 | 132 | 2.30e-21 | 89.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180006218.01.T01 | AT1G04880 | 44.706 | 170 | 63 | 7 | 1 | 170 | 1 | 139 | 8.15e-33 | 123 |
| MsG0180006218.01.T01 | AT1G76110 | 38.562 | 153 | 64 | 5 | 2 | 154 | 12 | 134 | 7.45e-23 | 94.7 |
| MsG0180006218.01.T01 | AT3G13350 | 40.800 | 125 | 47 | 4 | 29 | 151 | 34 | 133 | 1.46e-18 | 82.8 |
| MsG0180006218.01.T01 | AT1G55650 | 39.344 | 122 | 51 | 4 | 31 | 152 | 30 | 128 | 3.02e-18 | 82.0 |
Find 48 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGTTAGTAGTAATCTTATT+TGG | 0.190865 | 1:+101514052 | None:intergenic |
| TTATTGCAGGATTCTGTTAT+TGG | 0.209862 | 1:-101514890 | MsG0180006218.01.T01:intron |
| TATTGCAGGATTCTGTTATT+GGG | 0.284914 | 1:-101514889 | MsG0180006218.01.T01:intron |
| GAAGCATTTGTAGCCGTTGA+TGG | 0.327945 | 1:+101514394 | None:intergenic |
| AATAATAGATTTGTTCATAA+TGG | 0.330253 | 1:+101514335 | None:intergenic |
| GTATGTGAAAACTGCATCTT+TGG | 0.346993 | 1:+101514160 | None:intergenic |
| TAGTGATACAGTACCTTATC+TGG | 0.358483 | 1:-101514482 | MsG0180006218.01.T01:CDS |
| GGTTAGTAGTAATCTTATTT+GGG | 0.367147 | 1:+101514053 | None:intergenic |
| GCTCAAAGCTGTCTCTTTCA+TGG | 0.381404 | 1:+101515199 | None:intergenic |
| TGCATCTTTGGAGCAGGAGC+TGG | 0.387029 | 1:+101514172 | None:intergenic |
| AAAGCAAAGCAAAGACCTTC+AGG | 0.394236 | 1:+101514082 | None:intergenic |
| AATTCGTAGTTTATAAATCC+AGG | 0.415540 | 1:+101515059 | None:intergenic |
| GCTGTCTCTTTCATGGGTAA+TGG | 0.461532 | 1:+101515206 | None:intergenic |
| CAAGCTCTTCATACTTAGCT+TGG | 0.463066 | 1:-101515123 | MsG0180006218.01.T01:CDS |
| GAACATACCGGAAGTTGTAT+TGG | 0.464656 | 1:+101514294 | None:intergenic |
| AACTGGACCAATACAACTTC+CGG | 0.475197 | 1:-101514301 | MsG0180006218.01.T01:intron |
| CAAGACAGATTAACCAGATA+AGG | 0.480174 | 1:+101514469 | None:intergenic |
| TTCACCTATGGCAACATATG+AGG | 0.483062 | 1:-101515162 | MsG0180006218.01.T01:CDS |
| TACGAATTAGATATTGTACT+TGG | 0.490323 | 1:-101515044 | MsG0180006218.01.T01:intron |
| CAAGCTAAGTATGAAGAGCT+TGG | 0.497014 | 1:+101515124 | None:intergenic |
| CCAAGTTCATGTAGGCTCCC+TGG | 0.498106 | 1:-101515077 | MsG0180006218.01.T01:CDS |
| CTTTGGAGCAGGAGCTGGGA+TGG | 0.499860 | 1:+101514177 | None:intergenic |
| TAGTAATCTTATTTGGGCTG+AGG | 0.502978 | 1:+101514059 | None:intergenic |
| TTTAACTGTTGAAAGACAGC+TGG | 0.504737 | 1:+101514121 | None:intergenic |
| CCAGGGAGCCTACATGAACT+TGG | 0.508162 | 1:+101515077 | None:intergenic |
| TTTGTTGAAGTGACTTCTCG+TGG | 0.510774 | 1:-101514840 | MsG0180006218.01.T01:CDS |
| AAGCTAAGTATGAAGAGCTT+GGG | 0.516105 | 1:+101515125 | None:intergenic |
| GCATCTTTGGAGCAGGAGCT+GGG | 0.522935 | 1:+101514173 | None:intergenic |
| TGGTGAATATCCTTCACCTA+TGG | 0.528306 | 1:-101515174 | MsG0180006218.01.T01:CDS |
| GAAAACTGCATCTTTGGAGC+AGG | 0.538241 | 1:+101514166 | None:intergenic |
| CTCAAAGCTGTCTCTTTCAT+GGG | 0.539064 | 1:+101515200 | None:intergenic |
| ATTGCAGGATTCTGTTATTG+GGG | 0.539834 | 1:-101514888 | MsG0180006218.01.T01:intron |
| TTGAAGTGACTTCTCGTGGA+GGG | 0.541093 | 1:-101514836 | MsG0180006218.01.T01:intron |
| GTTAATTCTGCTCCGCCTGA+AGG | 0.550930 | 1:-101514097 | MsG0180006218.01.T01:CDS |
| TCATATGTTGCCATAGGTGA+AGG | 0.568033 | 1:+101515164 | None:intergenic |
| ATTCGTAGTTTATAAATCCA+GGG | 0.574160 | 1:+101515060 | None:intergenic |
| AAAGAGACAGCTTTGAGCCA+TGG | 0.588397 | 1:-101515194 | MsG0180006218.01.T01:CDS |
| GGAGAAGCTCCACACTCTCA+TGG | 0.590958 | 1:-101515102 | MsG0180006218.01.T01:CDS |
| AAGACAGCTGGTTGAACTTG+TGG | 0.608568 | 1:+101514133 | None:intergenic |
| TCATGGCACCAAGTTCATGT+AGG | 0.612383 | 1:-101515085 | MsG0180006218.01.T01:CDS |
| ACCTCCTCATATGTTGCCAT+AGG | 0.625420 | 1:+101515158 | None:intergenic |
| ACCTATGGCAACATATGAGG+AGG | 0.630539 | 1:-101515159 | MsG0180006218.01.T01:CDS |
| GTTGAAGTGACTTCTCGTGG+AGG | 0.634997 | 1:-101514837 | MsG0180006218.01.T01:CDS |
| AACTTGGTGCCATGAGAGTG+TGG | 0.647399 | 1:+101515093 | None:intergenic |
| GAGAAGTCACTTCAACAAAG+AGG | 0.662036 | 1:+101514844 | None:intergenic |
| GCAAAGCAAAGACCTTCAGG+CGG | 0.704080 | 1:+101514085 | None:intergenic |
| TTGCAGGATTCTGTTATTGG+GGG | 0.704677 | 1:-101514887 | MsG0180006218.01.T01:intron |
| TAGGTGAAGGATATTCACCA+TGG | 0.732815 | 1:+101515177 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATAATAGATTTGTTCATAA+TGG | + | Chr1:101514952-101514971 | None:intergenic | 15.0% |
| !!! | TGATTATAATTTCATTTTGT+TGG | - | Chr1:101514488-101514507 | MsG0180006218.01.T01:intron | 15.0% |
| !!! | TTACTTTTATTTTAAGATGT+GGG | - | Chr1:101514264-101514283 | MsG0180006218.01.T01:intron | 15.0% |
| !!! | TTTACTTTTATTTTAAGATG+TGG | - | Chr1:101514263-101514282 | MsG0180006218.01.T01:intron | 15.0% |
| !! | GATCATTAAAGATAGAAAAA+TGG | - | Chr1:101514840-101514859 | MsG0180006218.01.T01:CDS | 20.0% |
| !! | TATAAATTGGAAATTCACTA+TGG | - | Chr1:101515060-101515079 | MsG0180006218.01.T01:CDS | 20.0% |
| !! | TTACATATTCAAACAATGTT+TGG | + | Chr1:101514536-101514555 | None:intergenic | 20.0% |
| !! | TTCCAATTTATACATTAGAA+GGG | + | Chr1:101515052-101515071 | None:intergenic | 20.0% |
| !! | TTTCCAATTTATACATTAGA+AGG | + | Chr1:101515053-101515072 | None:intergenic | 20.0% |
| !!! | TATCTTTTGAATCATAACTT+TGG | - | Chr1:101514757-101514776 | MsG0180006218.01.T01:intron | 20.0% |
| ! | AATTCGTAGTTTATAAATCC+AGG | + | Chr1:101514228-101514247 | None:intergenic | 25.0% |
| ! | ATTCGTAGTTTATAAATCCA+GGG | + | Chr1:101514227-101514246 | None:intergenic | 25.0% |
| ! | TACGAATTAGATATTGTACT+TGG | - | Chr1:101514240-101514259 | MsG0180006218.01.T01:intron | 25.0% |
| ! | TCCAATTTATACATTAGAAG+GGG | + | Chr1:101515051-101515070 | None:intergenic | 25.0% |
| ! | TGCAAAGTTATACAAGTAAA+TGG | + | Chr1:101514352-101514371 | None:intergenic | 25.0% |
| ! | TGGTTAGTAGTAATCTTATT+TGG | + | Chr1:101515235-101515254 | None:intergenic | 25.0% |
| ! | TTGCAATTACAAAAAACCTT+TGG | - | Chr1:101514367-101514386 | MsG0180006218.01.T01:CDS | 25.0% |
| !! | ACTGATAATGTCCAAAATAT+AGG | - | Chr1:101514557-101514576 | MsG0180006218.01.T01:intron | 25.0% |
| !! | AGTTTTCAATTTTCCATCAA+CGG | - | Chr1:101514877-101514896 | MsG0180006218.01.T01:CDS | 25.0% |
| !! | GGTTAGTAGTAATCTTATTT+GGG | + | Chr1:101515234-101515253 | None:intergenic | 25.0% |
| ATCCTGCAATAAAATTCCAA+AGG | + | Chr1:101514386-101514405 | None:intergenic | 30.0% | |
| TATTGCAGGATTCTGTTATT+GGG | - | Chr1:101514395-101514414 | MsG0180006218.01.T01:CDS | 30.0% | |
| TTATTGCAGGATTCTGTTAT+TGG | - | Chr1:101514394-101514413 | MsG0180006218.01.T01:CDS | 30.0% | |
| ! | ATTTTGTTGGTATGAATGCA+TGG | - | Chr1:101514501-101514520 | MsG0180006218.01.T01:intron | 30.0% |
| !!! | AACCTTTGGAATTTTATTGC+AGG | - | Chr1:101514381-101514400 | MsG0180006218.01.T01:CDS | 30.0% |
| !!! | ATTATTTTAAAGCTCGCAAC+TGG | - | Chr1:101514966-101514985 | MsG0180006218.01.T01:intron | 30.0% |
| !!! | TTGGTTTTTTCCTACATGTT+TGG | - | Chr1:101514776-101514795 | MsG0180006218.01.T01:intron | 30.0% |
| AAGCTAAGTATGAAGAGCTT+GGG | + | Chr1:101514162-101514181 | None:intergenic | 35.0% | |
| ATCACTACAACCAAACATGT+AGG | + | Chr1:101514789-101514808 | None:intergenic | 35.0% | |
| ATTCAAACAATGTTTGGCGT+CGG | + | Chr1:101514530-101514549 | None:intergenic | 35.0% | |
| ATTGCAGGATTCTGTTATTG+GGG | - | Chr1:101514396-101514415 | MsG0180006218.01.T01:CDS | 35.0% | |
| CAAGACAGATTAACCAGATA+AGG | + | Chr1:101514818-101514837 | None:intergenic | 35.0% | |
| GCCCCTTCTAATGTATAAAT+TGG | - | Chr1:101515047-101515066 | MsG0180006218.01.T01:CDS | 35.0% | |
| GTATGTGAAAACTGCATCTT+TGG | + | Chr1:101515127-101515146 | None:intergenic | 35.0% | |
| TAGTGATACAGTACCTTATC+TGG | - | Chr1:101514802-101514821 | MsG0180006218.01.T01:intron | 35.0% | |
| TGTAGTTCCAATGAACATAC+CGG | + | Chr1:101515005-101515024 | None:intergenic | 35.0% | |
| TTTAACTGTTGAAAGACAGC+TGG | + | Chr1:101515166-101515185 | None:intergenic | 35.0% | |
| ! | TAGTAATCTTATTTGGGCTG+AGG | + | Chr1:101515228-101515247 | None:intergenic | 35.0% |
| !! | TTGCTGTTTGTACTAATGTC+AGG | - | Chr1:101515025-101515044 | MsG0180006218.01.T01:intron | 35.0% |
| AAAGCAAAGCAAAGACCTTC+AGG | + | Chr1:101515205-101515224 | None:intergenic | 40.0% | |
| AACTGGACCAATACAACTTC+CGG | - | Chr1:101514983-101515002 | MsG0180006218.01.T01:intron | 40.0% | |
| ACAACTTCCGGTATGTTCAT+TGG | - | Chr1:101514995-101515014 | MsG0180006218.01.T01:intron | 40.0% | |
| CAAGCTAAGTATGAAGAGCT+TGG | + | Chr1:101514163-101514182 | None:intergenic | 40.0% | |
| CAAGCTCTTCATACTTAGCT+TGG | - | Chr1:101514161-101514180 | MsG0180006218.01.T01:CDS | 40.0% | |
| CTCAAAGCTGTCTCTTTCAT+GGG | + | Chr1:101514087-101514106 | None:intergenic | 40.0% | |
| GAACATACCGGAAGTTGTAT+TGG | + | Chr1:101514993-101515012 | None:intergenic | 40.0% | |
| GAGAAGTCACTTCAACAAAG+AGG | + | Chr1:101514443-101514462 | None:intergenic | 40.0% | |
| GTCCAAAATATAGGACACTG+AGG | - | Chr1:101514566-101514585 | MsG0180006218.01.T01:intron | 40.0% | |
| TAGGTGAAGGATATTCACCA+TGG | + | Chr1:101514110-101514129 | None:intergenic | 40.0% | |
| TCATATGTTGCCATAGGTGA+AGG | + | Chr1:101514123-101514142 | None:intergenic | 40.0% | |
| TGGTGAATATCCTTCACCTA+TGG | - | Chr1:101514110-101514129 | MsG0180006218.01.T01:CDS | 40.0% | |
| TTCACCTATGGCAACATATG+AGG | - | Chr1:101514122-101514141 | MsG0180006218.01.T01:CDS | 40.0% | |
| TTGCAGGATTCTGTTATTGG+GGG | - | Chr1:101514397-101514416 | MsG0180006218.01.T01:CDS | 40.0% | |
| TTTGTTGAAGTGACTTCTCG+TGG | - | Chr1:101514444-101514463 | MsG0180006218.01.T01:CDS | 40.0% | |
| ! | CAAATGCTTCTTTTGTGCTG+CGG | - | Chr1:101514903-101514922 | MsG0180006218.01.T01:intron | 40.0% |
| AAGACAGCTGGTTGAACTTG+TGG | + | Chr1:101515154-101515173 | None:intergenic | 45.0% | |
| ACCTATGGCAACATATGAGG+AGG | - | Chr1:101514125-101514144 | MsG0180006218.01.T01:CDS | 45.0% | |
| ACCTCCTCATATGTTGCCAT+AGG | + | Chr1:101514129-101514148 | None:intergenic | 45.0% | |
| AGGATTCTGTTATTGGGGGA+AGG | - | Chr1:101514401-101514420 | MsG0180006218.01.T01:CDS | 45.0% | |
| GCTCAAAGCTGTCTCTTTCA+TGG | + | Chr1:101514088-101514107 | None:intergenic | 45.0% | |
| GCTGTCTCTTTCATGGGTAA+TGG | + | Chr1:101514081-101514100 | None:intergenic | 45.0% | |
| GGATTCTGTTATTGGGGGAA+GGG | - | Chr1:101514402-101514421 | MsG0180006218.01.T01:CDS | 45.0% | |
| TCATGGCACCAAGTTCATGT+AGG | - | Chr1:101514199-101514218 | MsG0180006218.01.T01:CDS | 45.0% | |
| TTGAAGTGACTTCTCGTGGA+GGG | - | Chr1:101514448-101514467 | MsG0180006218.01.T01:CDS | 45.0% | |
| ! | CGCCTCAGTGTCCTATATTT+TGG | + | Chr1:101514571-101514590 | None:intergenic | 45.0% |
| ! | GAAGCATTTGTAGCCGTTGA+TGG | + | Chr1:101514893-101514912 | None:intergenic | 45.0% |
| !! | AAAGAGACAGCTTTGAGCCA+TGG | - | Chr1:101514090-101514109 | MsG0180006218.01.T01:CDS | 45.0% |
| !! | GAAAACTGCATCTTTGGAGC+AGG | + | Chr1:101515121-101515140 | None:intergenic | 45.0% |
| CTGTTATTGGGGGAAGGGAT+TGG | - | Chr1:101514407-101514426 | MsG0180006218.01.T01:CDS | 50.0% | |
| GCAAAGCAAAGACCTTCAGG+CGG | + | Chr1:101515202-101515221 | None:intergenic | 50.0% | |
| GTTAATTCTGCTCCGCCTGA+AGG | - | Chr1:101515187-101515206 | MsG0180006218.01.T01:CDS | 50.0% | |
| GTTGAAGTGACTTCTCGTGG+AGG | - | Chr1:101514447-101514466 | MsG0180006218.01.T01:CDS | 50.0% | |
| !! | AACTTGGTGCCATGAGAGTG+TGG | + | Chr1:101514194-101514213 | None:intergenic | 50.0% |
| CCAAGTTCATGTAGGCTCCC+TGG | - | Chr1:101514207-101514226 | MsG0180006218.01.T01:intron | 55.0% | |
| CCAGGGAGCCTACATGAACT+TGG | + | Chr1:101514210-101514229 | None:intergenic | 55.0% | |
| GGAGAAGCTCCACACTCTCA+TGG | - | Chr1:101514182-101514201 | MsG0180006218.01.T01:CDS | 55.0% | |
| !! | GCATCTTTGGAGCAGGAGCT+GGG | + | Chr1:101515114-101515133 | None:intergenic | 55.0% |
| !! | TGCATCTTTGGAGCAGGAGC+TGG | + | Chr1:101515115-101515134 | None:intergenic | 55.0% |
| !! | CTTTGGAGCAGGAGCTGGGA+TGG | + | Chr1:101515110-101515129 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 101514048 | 101515258 | 101514048 | ID=MsG0180006218.01;Name=MsG0180006218.01 |
| Chr1 | mRNA | 101514048 | 101515258 | 101514048 | ID=MsG0180006218.01.T01;Parent=MsG0180006218.01;Name=MsG0180006218.01.T01;_AED=0.53;_eAED=0.53;_QI=0|0.33|0|1|0.66|0.75|4|0|217 |
| Chr1 | exon | 101515045 | 101515258 | 101515045 | ID=MsG0180006218.01.T01:exon:15092;Parent=MsG0180006218.01.T01 |
| Chr1 | exon | 101514837 | 101514903 | 101514837 | ID=MsG0180006218.01.T01:exon:15091;Parent=MsG0180006218.01.T01 |
| Chr1 | exon | 101514302 | 101514501 | 101514302 | ID=MsG0180006218.01.T01:exon:15090;Parent=MsG0180006218.01.T01 |
| Chr1 | exon | 101514048 | 101514220 | 101514048 | ID=MsG0180006218.01.T01:exon:15089;Parent=MsG0180006218.01.T01 |
| Chr1 | CDS | 101515045 | 101515258 | 101515045 | ID=MsG0180006218.01.T01:cds;Parent=MsG0180006218.01.T01 |
| Chr1 | CDS | 101514837 | 101514903 | 101514837 | ID=MsG0180006218.01.T01:cds;Parent=MsG0180006218.01.T01 |
| Chr1 | CDS | 101514302 | 101514501 | 101514302 | ID=MsG0180006218.01.T01:cds;Parent=MsG0180006218.01.T01 |
| Chr1 | CDS | 101514048 | 101514220 | 101514048 | ID=MsG0180006218.01.T01:cds;Parent=MsG0180006218.01.T01 |
| Gene Sequence |
| Protein sequence |
