AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041254.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041254.01.T01 MTR_7g107680 85.165 182 20 2 1 175 1 182 7.51e-100 285
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041254.01.T01 AT4G03150 52.121 165 74 2 10 169 17 181 4.68e-52 165

Find 55 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAGGGTTACTTTACATAAA+TGG 0.225005 7:-88833503 MsG0780041254.01.T01:CDS
ATTGGAAACCATTTCTTATA+AGG 0.243835 7:-88833352 MsG0780041254.01.T01:CDS
TGGAGCTATGAAATTCAATT+TGG 0.253108 7:+88833252 None:intergenic
GTTCAATCCGCTGCGCGATT+AGG 0.295554 7:+88833286 None:intergenic
ACTTGGTCGTCATCGGGTTC+TGG 0.304693 7:+88833910 None:intergenic
CAAAGAATTGTAGTAGGATT+TGG 0.316745 7:+88833425 None:intergenic
ATTAGGAATCAGAGTGGCTT+AGG 0.341814 7:-88833521 MsG0780041254.01.T01:intron
GAGGAGGAAGATGGCGTGAA+TGG 0.349139 7:+88834030 None:intergenic
AAAACTTTCCTTATAAGAAA+TGG 0.350129 7:+88833344 None:intergenic
ATCTCCACATTTGTTTCCTC+AGG 0.391134 7:+88833462 None:intergenic
GTTGAATATGGCACGTGAAT+TGG 0.399859 7:-88833370 MsG0780041254.01.T01:CDS
GTCTTCAACTTGGTCGTCAT+CGG 0.400136 7:+88833903 None:intergenic
TCGTCATCGGGTTCTGGAAA+TGG 0.412464 7:+88833916 None:intergenic
GTGAAGAGGAAGCGTGTTGA+TGG 0.422901 7:+88833980 None:intergenic
GGTGAGTGTAACGGAGGAAG+AGG 0.428456 7:+88834005 None:intergenic
GAAGAGGAGGAGGAGGAAGA+TGG 0.434780 7:+88834021 None:intergenic
GATAGAATGATGGGATGAAC+TGG 0.437699 7:+88834069 None:intergenic
AGGAGGAAGATGGCGTGAAT+GGG 0.452880 7:+88834031 None:intergenic
AACGGAGGAAGAGGAGGAGG+AGG 0.453024 7:+88834014 None:intergenic
TCTTCAACTTGGTCGTCATC+GGG 0.460270 7:+88833904 None:intergenic
TGTAACGGAGGAAGAGGAGG+AGG 0.463568 7:+88834011 None:intergenic
CCGTATCAAAGAATTGTAGT+AGG 0.472999 7:+88833419 None:intergenic
GAGTGTAACGGAGGAAGAGG+AGG 0.477450 7:+88834008 None:intergenic
CTACCTGGGAGGCATTGGTT+GGG 0.478796 7:+88833854 None:intergenic
AGTACCTACCTGGGAGGCAT+TGG 0.485202 7:+88833849 None:intergenic
GGAGCTATGAAATTCAATTT+GGG 0.488254 7:+88833253 None:intergenic
CGTGAATGGGATGATGCTGC+GGG 0.502866 7:+88834044 None:intergenic
GGTTACTTTACATAAATGGT+TGG 0.503773 7:-88833499 MsG0780041254.01.T01:CDS
GAAATGGATGAGAATGTGAA+TGG 0.509383 7:+88833932 None:intergenic
CCTACTACAATTCTTTGATA+CGG 0.511408 7:-88833419 MsG0780041254.01.T01:CDS
AAGAGGAAGCGTGTTGATGG+TGG 0.520055 7:+88833983 None:intergenic
TTAGGAATCAGAGTGGCTTA+GGG 0.523126 7:-88833520 MsG0780041254.01.T01:intron
GCGTGAATGGGATGATGCTG+CGG 0.532208 7:+88834043 None:intergenic
ACTCCCAACCAATGCCTCCC+AGG 0.538117 7:-88833857 MsG0780041254.01.T01:intron
CCTACCTGGGAGGCATTGGT+TGG 0.555347 7:+88833853 None:intergenic
GCTGCGGGGTGATAGAATGA+TGG 0.566571 7:+88834059 None:intergenic
GAACACGAAGGTCTTCAACT+TGG 0.573094 7:+88833893 None:intergenic
TGGTTGGGAGTAACCAATGA+GGG 0.597474 7:+88833869 None:intergenic
AGCCGCATTTGCAGATGTGT+AGG 0.600800 7:+88833314 None:intergenic
CTGTCCTGAGGAAACAAATG+TGG 0.608003 7:-88833466 MsG0780041254.01.T01:CDS
GCTATGAAATTCAATTTGGG+TGG 0.608458 7:+88833256 None:intergenic
CTGCGGGGTGATAGAATGAT+GGG 0.608917 7:+88834060 None:intergenic
AGAGGAAGCGTGTTGATGGT+GGG 0.631850 7:+88833984 None:intergenic
GGCCTACACATCTGCAAATG+CGG 0.641836 7:-88833316 MsG0780041254.01.T01:CDS
AAATGGATGAGAATGTGAAT+GGG 0.656050 7:+88833933 None:intergenic
TTGATGGTGGGTGAGTGTAA+CGG 0.662684 7:+88833996 None:intergenic
TTGGTTGGGAGTAACCAATG+AGG 0.663293 7:+88833868 None:intergenic
ACAATTATTAGGAATCAGAG+TGG 0.674841 7:-88833527 MsG0780041254.01.T01:intron
CTGTAAACCTAATCGCGCAG+CGG 0.684704 7:-88833293 MsG0780041254.01.T01:CDS
ATGGTGGGTGAGTGTAACGG+AGG 0.695017 7:+88833999 None:intergenic
GTTGGGAGTAACCAATGAGG+GGG 0.704622 7:+88833871 None:intergenic
GTGAATGGGATGATGCTGCG+GGG 0.706574 7:+88834045 None:intergenic
GGTTGGGAGTAACCAATGAG+GGG 0.709296 7:+88833870 None:intergenic
GGATGATGAATACTGTCCTG+AGG 0.725475 7:-88833478 MsG0780041254.01.T01:CDS
GAAAAGAGTACCTACCTGGG+AGG 0.727204 7:+88833843 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACTTTCCTTATAAGAAA+TGG + Chr7:88834015-88834034 None:intergenic 20.0%
!!! CTATTTTCTCAAATTATTAC+CGG + Chr7:88833677-88833696 None:intergenic 20.0%
!!! TTTTTCCATTCACAATTATT+AGG - Chr7:88833818-88833837 MsG0780041254.01.T01:intron 20.0%
! ATTGGAAACCATTTCTTATA+AGG - Chr7:88834004-88834023 MsG0780041254.01.T01:CDS 25.0%
! TGATTCCTAATAATTGTGAA+TGG + Chr7:88833826-88833845 None:intergenic 25.0%
!! CTTATAAGGAAAGTTTTCAT+GGG - Chr7:88834018-88834037 MsG0780041254.01.T01:CDS 25.0%
!! TCTTATAAGGAAAGTTTTCA+TGG - Chr7:88834017-88834036 MsG0780041254.01.T01:CDS 25.0%
!! TTAGGGTTACTTTACATAAA+TGG - Chr7:88833853-88833872 MsG0780041254.01.T01:intron 25.0%
!! TTATAAGGAAAGTTTTCATG+GGG - Chr7:88834019-88834038 MsG0780041254.01.T01:CDS 25.0%
!!! ACATGTTTCTAAAATTTGAG+AGG + Chr7:88833550-88833569 None:intergenic 25.0%
AAATGGATGAGAATGTGAAT+GGG + Chr7:88833426-88833445 None:intergenic 30.0%
ACAATTATTAGGAATCAGAG+TGG - Chr7:88833829-88833848 MsG0780041254.01.T01:intron 30.0%
CAAAGAATTGTAGTAGGATT+TGG + Chr7:88833934-88833953 None:intergenic 30.0%
CCTACTACAATTCTTTGATA+CGG - Chr7:88833937-88833956 MsG0780041254.01.T01:CDS 30.0%
! AGCTGACATTTTGTTGAATA+TGG - Chr7:88833974-88833993 MsG0780041254.01.T01:CDS 30.0%
! GGTTACTTTACATAAATGGT+TGG - Chr7:88833857-88833876 MsG0780041254.01.T01:intron 30.0%
!! TTTCTAAAATTTGAGAGGAG+TGG + Chr7:88833545-88833564 None:intergenic 30.0%
ATGAAGATATAAATGAGCGC+AGG + Chr7:88833788-88833807 None:intergenic 35.0%
CCGTATCAAAGAATTGTAGT+AGG + Chr7:88833940-88833959 None:intergenic 35.0%
GAAATGGATGAGAATGTGAA+TGG + Chr7:88833427-88833446 None:intergenic 35.0%
TACTTGTAGCTGAGATGTTA+AGG - Chr7:88833575-88833594 MsG0780041254.01.T01:intron 35.0%
! CGAGTAGTGTGAACAATTTT+TGG + Chr7:88833401-88833420 None:intergenic 35.0%
!! AATGAAAAGAGTACCTACCT+GGG + Chr7:88833519-88833538 None:intergenic 35.0%
!!! GTGAACAATTTTTGGTGAAG+AGG + Chr7:88833393-88833412 None:intergenic 35.0%
ATCTCCACATTTGTTTCCTC+AGG + Chr7:88833897-88833916 None:intergenic 40.0%
ATTAGGAATCAGAGTGGCTT+AGG - Chr7:88833835-88833854 MsG0780041254.01.T01:intron 40.0%
ATTTCAACACCGAAAGGACA+CGG + Chr7:88833730-88833749 None:intergenic 40.0%
CTAGTGATTTCAACACCGAA+AGG + Chr7:88833736-88833755 None:intergenic 40.0%
GTTGAAATCACTAGGTGGTT+AGG - Chr7:88833742-88833761 MsG0780041254.01.T01:intron 40.0%
GTTGAATATGGCACGTGAAT+TGG - Chr7:88833986-88834005 MsG0780041254.01.T01:CDS 40.0%
TTAGGAATCAGAGTGGCTTA+GGG - Chr7:88833836-88833855 MsG0780041254.01.T01:intron 40.0%
! GATAGAATGATGGGATGAAC+TGG + Chr7:88833290-88833309 None:intergenic 40.0%
!! CTTTCGGTGTTGAAATCACT+AGG - Chr7:88833734-88833753 MsG0780041254.01.T01:intron 40.0%
!! GAATGAAAAGAGTACCTACC+TGG + Chr7:88833520-88833539 None:intergenic 40.0%
CTGTCCTGAGGAAACAAATG+TGG - Chr7:88833890-88833909 MsG0780041254.01.T01:CDS 45.0%
GAACACGAAGGTCTTCAACT+TGG + Chr7:88833466-88833485 None:intergenic 45.0%
GGATGATGAATACTGTCCTG+AGG - Chr7:88833878-88833897 MsG0780041254.01.T01:CDS 45.0%
GTCTTCAACTTGGTCGTCAT+CGG + Chr7:88833456-88833475 None:intergenic 45.0%
TCTTCAACTTGGTCGTCATC+GGG + Chr7:88833455-88833474 None:intergenic 45.0%
TGGTTGGGAGTAACCAATGA+GGG + Chr7:88833490-88833509 None:intergenic 45.0%
TTGGTTGGGAGTAACCAATG+AGG + Chr7:88833491-88833510 None:intergenic 45.0%
! TAGTTAGCTTAGACCGACGA+CGG - Chr7:88833640-88833659 MsG0780041254.01.T01:intron 45.0%
!! TCGGTGTTGAAATCACTAGG+TGG - Chr7:88833737-88833756 MsG0780041254.01.T01:intron 45.0%
!! TTGATGGTGGGTGAGTGTAA+CGG + Chr7:88833363-88833382 None:intergenic 45.0%
AGCCGCATTTGCAGATGTGT+AGG + Chr7:88834045-88834064 None:intergenic 50.0%
AGGAGGAAGATGGCGTGAAT+GGG + Chr7:88833328-88833347 None:intergenic 50.0%
CACATGTGTCCGTGTCCTTT+CGG - Chr7:88833718-88833737 MsG0780041254.01.T01:intron 50.0%
CTGCGGGGTGATAGAATGAT+GGG + Chr7:88833299-88833318 None:intergenic 50.0%
CTGTAAACCTAATCGCGCAG+CGG - Chr7:88834063-88834082 MsG0780041254.01.T01:CDS 50.0%
GGCCTACACATCTGCAAATG+CGG - Chr7:88834040-88834059 MsG0780041254.01.T01:CDS 50.0%
GGTTGGGAGTAACCAATGAG+GGG + Chr7:88833489-88833508 None:intergenic 50.0%
GTTGGGAGTAACCAATGAGG+GGG + Chr7:88833488-88833507 None:intergenic 50.0%
! AAGAGGAAGCGTGTTGATGG+TGG + Chr7:88833376-88833395 None:intergenic 50.0%
! GTGAAGAGGAAGCGTGTTGA+TGG + Chr7:88833379-88833398 None:intergenic 50.0%
!! AGAGGAAGCGTGTTGATGGT+GGG + Chr7:88833375-88833394 None:intergenic 50.0%
!! GAAAAGAGTACCTACCTGGG+AGG + Chr7:88833516-88833535 None:intergenic 50.0%
!! TCGTCATCGGGTTCTGGAAA+TGG + Chr7:88833443-88833462 None:intergenic 50.0%
ACCAATGAGGGGGAACACGA+AGG + Chr7:88833478-88833497 None:intergenic 55.0%
ACCTTCGTGTTCCCCCTCAT+TGG - Chr7:88833474-88833493 MsG0780041254.01.T01:CDS 55.0%
ACTTGGTCGTCATCGGGTTC+TGG + Chr7:88833449-88833468 None:intergenic 55.0%
CGTGAATGGGATGATGCTGC+GGG + Chr7:88833315-88833334 None:intergenic 55.0%
GAAGAGGAGGAGGAGGAAGA+TGG + Chr7:88833338-88833357 None:intergenic 55.0%
GAGGAGGAAGATGGCGTGAA+TGG + Chr7:88833329-88833348 None:intergenic 55.0%
GCGTGAATGGGATGATGCTG+CGG + Chr7:88833316-88833335 None:intergenic 55.0%
GCTGCGGGGTGATAGAATGA+TGG + Chr7:88833300-88833319 None:intergenic 55.0%
GTGAATGGGATGATGCTGCG+GGG + Chr7:88833314-88833333 None:intergenic 55.0%
GTTCAATCCGCTGCGCGATT+AGG + Chr7:88834073-88834092 None:intergenic 55.0%
! AGTACCTACCTGGGAGGCAT+TGG + Chr7:88833510-88833529 None:intergenic 55.0%
! GAGTGTAACGGAGGAAGAGG+AGG + Chr7:88833351-88833370 None:intergenic 55.0%
! GGTGAGTGTAACGGAGGAAG+AGG + Chr7:88833354-88833373 None:intergenic 55.0%
! TGTAACGGAGGAAGAGGAGG+AGG + Chr7:88833348-88833367 None:intergenic 55.0%
!! ATGGTGGGTGAGTGTAACGG+AGG + Chr7:88833360-88833379 None:intergenic 55.0%
!! CTACCTGGGAGGCATTGGTT+GGG + Chr7:88833505-88833524 None:intergenic 55.0%
AACGGAGGAAGAGGAGGAGG+AGG + Chr7:88833345-88833364 None:intergenic 60.0%
ACTCCCAACCAATGCCTCCC+AGG - Chr7:88833499-88833518 MsG0780041254.01.T01:CDS 60.0%
CCAACCAATGCCTCCCAGGT+AGG - Chr7:88833503-88833522 MsG0780041254.01.T01:CDS 60.0%
GACGACGGACACGTCAACAC+CGG - Chr7:88833655-88833674 MsG0780041254.01.T01:intron 60.0%
!! CCTACCTGGGAGGCATTGGT+TGG + Chr7:88833506-88833525 None:intergenic 60.0%
!! GGTGTTGACGTGTCCGTCGT+CGG + Chr7:88833656-88833675 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 88833266 88834112 88833266 ID=MsG0780041254.01;Name=MsG0780041254.01
Chr7 mRNA 88833266 88834112 88833266 ID=MsG0780041254.01.T01;Parent=MsG0780041254.01;Name=MsG0780041254.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|0|0.5|2|0|175
Chr7 exon 88833858 88834112 88833858 ID=MsG0780041254.01.T01:exon:12247;Parent=MsG0780041254.01.T01
Chr7 exon 88833266 88833538 88833266 ID=MsG0780041254.01.T01:exon:12246;Parent=MsG0780041254.01.T01
Chr7 CDS 88833858 88834112 88833858 ID=MsG0780041254.01.T01:cds;Parent=MsG0780041254.01.T01
Chr7 CDS 88833266 88833538 88833266 ID=MsG0780041254.01.T01:cds;Parent=MsG0780041254.01.T01
Gene Sequence

>MsG0780041254.01.T01

ATGTCAAGCTTGAAATTGAATCCAGTTCATCCCATCATTCTATCACCCCGCAGCATCATCCCATTCACGCCATCTTCCTCCTCCTCCTCTTCCTCCGTTACACTCACCCACCATCAACACGCTTCCTCTTCACCAAAAATTGTTCACACTACTCGTACCCATTCACATTCTCATCCATTTCCAGAACCCGATGACGACCAAGTTGAAGACCTTCGTGTTCCCCCTCATTGGTTACTCCCAACCAATGCCTCCCAGGAATCAGAGTGGCTTAGGGTTACTTTACATAAATGGTTGGATGATGAATACTGTCCTGAGGAAACAAATGTGGAGATAAGCAGAGTTGCTGCCAAATCCTACTACAATTCTTTGATACGGAAACAAGCTGATTTAGCTGACATTTTGTTGAATATGGCACGTGAATTGGAAACCATTTCTTATAAGGAAAGTTTTCATGGGGCCTACACATCTGCAAATGCGGCTGTAAACCTAATCGCGCAGCGGATTGAACAATTCTGCCACCCAAATTGA

Protein sequence

>MsG0780041254.01.T01

MSSLKLNPVHPIILSPRSIIPFTPSSSSSSSSVTLTHHQHASSSPKIVHTTRTHSHSHPFPEPDDDQVEDLRVPPHWLLPTNASQESEWLRVTLHKWLDDEYCPEETNVEISRVAAKSYYNSLIRKQADLADILLNMARELETISYKESFHGAYTSANAAVNLIAQRIEQFCHPN*