AlfalfaGEDB | Msa0336630

M. sativa cultivar zhongmu-4 / Msa0336630

KEGG: -
GO: -
PFAM: PF02298.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0336630	XP_003597041.1	94.298	228	13	0	1	228	1	228	4.37e-145	414

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0336630	sp\|O82081\|UCC1_ARATH	49.254	134	64	4	1	131	1	133	7.22e-42	145

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0004580	Msa0336630	0.833359	5.581366e-56	-8.615850e-47
Msa0024280	Msa0336630	0.808200	3.480529e-50	-8.615850e-47
Msa0024290	Msa0336630	0.815881	7.365151e-52	-8.615850e-47
Msa0043120	Msa0336630	0.809163	2.167492e-50	-8.615850e-47
Msa0048950	Msa0336630	0.843617	1.254923e-58	-8.615850e-47
Msa0059880	Msa0336630	0.828970	6.684581e-55	-8.615850e-47
Msa0061600	Msa0336630	0.810937	8.992732e-51	-8.615850e-47
Msa0072030	Msa0336630	0.848114	7.534891e-60	-8.615850e-47
Msa0073970	Msa0336630	0.804237	2.378632e-49	-8.615850e-47
Msa0074620	Msa0336630	0.816745	4.719912e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0336630	MtrunA17_Chr2g0323021	94.298	228	13	0	1	228	1	228	4.02e-149	414
Msa0336630	MtrunA17_Chr6g0481491	42.017	119	68	1	4	122	7	124	1.57e-34	122
Msa0336630	MtrunA17_Chr8g0335571	44.444	117	60	3	11	123	8	123	2.53e-30	111
Msa0336630	MtrunA17_Chr8g0335531	45.299	117	59	3	11	123	8	123	1.13e-29	109
Msa0336630	MtrunA17_Chr1g0196801	46.392	97	51	1	22	118	20	115	2.00e-29	108
Msa0336630	MtrunA17_Chr6g0459441	47.059	102	52	2	18	118	19	119	1.03e-27	103
Msa0336630	MtrunA17_Chr3g0132431	42.857	133	68	5	12	141	12	139	2.57e-27	103
Msa0336630	MtrunA17_Chr3g0132441	45.918	98	52	1	21	118	22	118	2.59e-25	98.2
Msa0336630	MtrunA17_Chr8g0380901	40.000	115	64	4	6	119	9	119	1.78e-24	94.0
Msa0336630	MtrunA17_Chr3g0132701	42.975	121	65	4	1	118	1	120	3.41e-24	95.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0336630	AT2G32300.1	49.254	134	64	4	1	131	1	133	7.34e-43	145
Msa0336630	AT2G32300.2	49.254	134	64	4	1	131	31	163	3.09e-42	145
Msa0336630	AT3G27200.1	38.136	118	70	3	6	121	6	122	1.85e-29	108
Msa0336630	AT5G07475.1	36.842	152	86	3	8	150	13	163	4.30e-28	105
Msa0336630	AT3G60270.1	49.474	95	48	0	29	123	29	123	5.68e-28	105
Msa0336630	AT5G53870.2	34.225	187	106	5	7	177	12	197	5.03e-26	103
Msa0336630	AT5G53870.1	34.225	187	106	5	7	177	12	197	7.73e-26	103
Msa0336630	AT2G25060.1	38.849	139	74	5	15	142	21	159	3.55e-25	97.8
Msa0336630	AT2G31050.1	39.844	128	69	3	12	131	10	137	1.09e-24	97.1
Msa0336630	AT2G26720.1	37.903	124	70	2	10	126	8	131	3.76e-24	95.9

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Find 62 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTCAAACCTTCAATCAT+GGG	0.565295	2_4:+56040499	Msa0336630:CDS
AACAAAGGACTTGGCGCTTC+AGG	0.262695	2_4:-56041052	None:intergenic
AACAATGTGATTTGTAGCCA+TGG	0.542196	2_4:-56040450	None:intergenic
AACTCAGTGGATGGAATCAT+TGG	0.412975	2_4:-56041106	None:intergenic
AATAGTGTTGAGTTTAGTAG+CGG	0.615409	2_4:+56041216	Msa0336630:CDS
AATTGGATTTGTTTGCTGGC+AGG	0.504490	2_4:-56040792	None:intergenic
ACAACATTCACAGGATGTAT+CGG	0.591172	2_4:+56041153	Msa0336630:CDS
ACAATAGGACATTGCAGCCA+AGG	0.536238	2_4:+56040884	Msa0336630:CDS
ACATTGCAGCCAAGGAATGA+AGG	0.543328	2_4:+56040892	Msa0336630:CDS
ACCATTCCTCTCACATCAAC+AGG	0.409662	2_4:+56040839	Msa0336630:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACTCAAACCTTCAATCAT+GGG	+	chr2_4:56040499-56040518	Msa0336630:CDS	30.0%
TACTTCATTTGTGGAACAAT+AGG	+	chr2_4:56040869-56040888	Msa0336630:CDS	30.0%
AAACTCAAACCTTCAATCAT+GGG	+	chr2_4:56040499-56040518	Msa0336630:CDS	30.0%
TACTTCATTTGTGGAACAAT+AGG	+	chr2_4:56040869-56040888	Msa0336630:CDS	30.0%
TGTGGCTATGCTAATCAAAT+TGG	+	chr2_4:56040427-56040446	Msa0336630:CDS	35.0%
TATGCTAATCAAATTGGCCA+TGG	+	chr2_4:56040433-56040452	Msa0336630:CDS	35.0%
ATGGCTACAAATCACATTGT+TGG	+	chr2_4:56040452-56040471	Msa0336630:CDS	35.0%
CAAACTCAAACCTTCAATCA+TGG	+	chr2_4:56040498-56040517	Msa0336630:CDS	35.0%
TTGTAACTTCAACCACATCA+TGG	-	chr2_4:56040757-56040776	None:intergenic	35.0%
CCATCATTGTAGGATTGAAT+TGG	-	chr2_4:56040812-56040831	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	56040392	56041258	56040392	ID=Msa0336630;Name=Msa0336630
chr2_4	mRNA	56040392	56041258	56040392	ID=Msa0336630-mRNA-1;Parent=Msa0336630;Name=Msa0336630-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0\|0\|0\|1\|1\|1\|2\|0\|229
chr2_4	exon	56040392	56040557	56040392	ID=Msa0336630-mRNA-1:exon:17062;Parent=Msa0336630-mRNA-1
chr2_4	exon	56040735	56041258	56040735	ID=Msa0336630-mRNA-1:exon:17063;Parent=Msa0336630-mRNA-1
chr2_4	CDS	56040392	56040557	56040392	ID=Msa0336630-mRNA-1:cds;Parent=Msa0336630-mRNA-1
chr2_4	CDS	56040735	56041258	56040735	ID=Msa0336630-mRNA-1:cds;Parent=Msa0336630-mRNA-1

Gene Sequence

>Msa0336630

ATGGGTGTACTTGAAATCATTCTAAGAATAACTTTTGTGGCTATGCTAATCAAATTGGCCATGGCTACAAATCACATTGTTGGAGGACCAAATGGTGGGTGGGATACAAACTCAAACCTTCAATCATGGGCATCTTCCCAGCAATTTTCAGTGGGAGACAATCTTATTTTCCAGTATCCACCAAACCATGATGTGGTTGAAGTTACAAAAGCAGACTATGACTCCTGCCAGCAAACAAATCCAATTCAATCCTACAATGATGGTGCCACAACCATTCCTCTCACATCAACAGGCAAAAGATACTTCATTTGTGGAACAATAGGACATTGCAGCCAAGGAATGAAGGTTGAAATCGACACACTTGCTGCTCAAGTTTCTCCTGCCTCACCAGTAGCAGCAGAACCTTCAATAGCAGACTCTCCAATGATTTCCATCATTCCCTCTGCAGCACCTGCAGAATCTACTGTTTCATCTGCAGAATCTCCTGAAGCGCCAAGTCCTTTGTTTGAAACACAAGCAGAAAGTCCTACACTCTCTCCAATGATTCCATCCACTGAGTTTCTAGCTCCTTCCTCTCCTATAGCACAACATTCACAGGATGTATCGGCCTCATCGACCGAGAAAGGAAATCTACAAGCCTCCATTTCAATAGTGTTGAGTTTAGTAGCGGTGTTTATGACCTTCTTCTAA

Protein sequence

>Msa0336630

MGVLEIILRITFVAMLIKLAMATNHIVGGPNGGWDTNSNLQSWASSQQFSVGDNLIFQYPPNHDVVEVTKADYDSCQQTNPIQSYNDGATTIPLTSTGKRYFICGTIGHCSQGMKVEIDTLAAQVSPASPVAAEPSIADSPMISIIPSAAPAESTVSSAESPEAPSPLFETQAESPTLSPMIPSTEFLAPSSPIAQHSQDVSASSTEKGNLQASISIVLSLVAVFMTFF*