AlfalfaGEDB | Msa0686670

M. sativa cultivar zhongmu-4 / Msa0686670

KEGG: K18660
GO: GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0008150 GO:0008152 GO:0008610 GO:0009056 GO:0009058 GO:0009987 GO:0016020 GO:0016053 GO:0016054 GO:0016405 GO:0016874 GO:0016877 GO:0016878 GO:0019752 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043649 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044282 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046395 GO:0071704 GO:0072330 GO:0090409 GO:0090410 GO:1901575 GO:1901576
PFAM: PF00501.31

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0686670	KEH28803.1	95.332	557	16	2	1	547	1	557	0.0	1088

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0686670	sp\|M4IS90\|CCL8_HUMLU	61.139	597	159	5	23	547	10	605	0.0	701

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0686670	A0A072UI68	95.332	557	16	2	1	547	1	557	0.0	1088

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001550	Msa0686670	-0.803273	3.771721e-49	-8.615850e-47
Msa0027020	Msa0686670	-0.816877	4.407761e-52	-8.615850e-47
Msa0074860	Msa0686670	-0.810172	1.315389e-50	-8.615850e-47
Msa0075990	Msa0686670	-0.809753	1.619375e-50	-8.615850e-47
Msa0087550	Msa0686670	0.826535	2.572168e-54	-8.615850e-47
Msa0093360	Msa0686670	-0.801041	1.085583e-48	-8.615850e-47
Msa0112550	Msa0686670	-0.827035	1.953816e-54	-8.615850e-47
Msa0134270	Msa0686670	0.820885	5.413755e-53	-8.615850e-47
Msa0158890	Msa0686670	-0.804163	2.464378e-49	-8.615850e-47
Msa0167160	Msa0686670	-0.818456	1.942240e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0686670	MtrunA17_Chr4g0004841	96.764	309	6	1	243	547	32	340	0.0	617
Msa0686670	MtrunA17_Chr4g0004101	81.957	327	42	4	234	547	9	331	0.0	545
Msa0686670	MtrunA17_Chr4g0004831	91.373	255	16	1	1	249	1	255	1.33e-156	448
Msa0686670	MtrunA17_Chr4g0004091	81.592	201	36	1	49	249	61	260	4.28e-111	332
Msa0686670	MtrunA17_Chr6g0471921	26.199	542	328	18	46	546	4	514	1.11e-38	148
Msa0686670	MtrunA17_Chr4g0028411	28.099	363	205	12	216	539	189	534	1.84e-29	122
Msa0686670	MtrunA17_Chr1g0180541	25.316	395	223	14	191	539	172	540	9.65e-27	114
Msa0686670	MtrunA17_Chr5g0409791	26.862	376	213	12	215	546	210	567	1.54e-26	113
Msa0686670	MtrunA17_Chr4g0039791	26.720	378	211	12	215	546	203	560	7.41e-26	111
Msa0686670	MtrunA17_Chr1g0157401	29.255	376	211	16	211	546	199	559	7.44e-26	111

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0686670	AT3G16170.1	58.638	602	177	8	6	547	19	608	0.0	672
Msa0686670	AT3G48990.1	27.699	491	282	16	89	537	45	504	5.01e-42	158
Msa0686670	AT4G19010.1	27.467	375	208	13	216	545	205	560	1.56e-30	125
Msa0686670	AT4G05160.1	26.703	367	217	12	210	539	185	536	8.13e-28	117
Msa0686670	AT1G20500.1	28.997	369	218	12	211	546	192	549	2.25e-27	116
Msa0686670	AT1G20510.1	28.032	371	219	14	211	545	184	542	2.97e-27	116
Msa0686670	AT1G20480.1	26.954	371	222	14	211	546	208	564	5.08e-26	112
Msa0686670	AT1G68270.1	27.809	356	215	11	212	542	183	521	3.32e-24	106
Msa0686670	AT1G65060.1	23.729	531	302	17	74	544	74	561	6.03e-24	106
Msa0686670	AT5G36880.6	28.084	381	214	19	214	547	301	668	2.61e-23	104

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Find 139 sgRNAs with CRISPR-Local

Find 629 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCATTACCCGGAGTGC+AGG	0.524416	4_4:+51239478	Msa0686670:CDS
AAAACCTAGGGAAATCTTGG+TGG	0.558124	4_4:+51236973	Msa0686670:intron
AAAGAATATGGAGAAATTGT+TGG	0.453282	4_4:+51242320	Msa0686670:CDS
AAATCATAACAATGTTCAAA+TGG	0.462971	4_4:-51244330	None:intergenic
AACAAATTCAGCAGAGGGTT+TGG	0.342190	4_4:-51237016	None:intergenic
AACAGGTTGAGGAAGTGCAG+AGG	0.703474	4_4:-51239252	None:intergenic
AACGACTTATTGGCAACAGG+TGG	0.637281	4_4:-51237570	None:intergenic
AACTCTTCTAGGCTTAGAGC+CGG	0.531743	4_4:-51242398	None:intergenic
AAGATATCTAATCTCTTATG+TGG	0.526973	4_4:+51236026	Msa0686670:CDS
AAGCAAGACCTTGCATCCCA+TGG	0.541759	4_4:+51235939	Msa0686670:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCAAAAAAATTCCCGAGAAA+CGG	-	chr4_4:51234786-51234805	None:intergenic	30.0%
GGTTATCTGACAAATTGTTT+AGG	-	chr4_4:51234844-51234863	None:intergenic	30.0%
CTGTATTCATATAAGCTACA+AGG	+	chr4_4:51234942-51234961	Msa0686670:intron	30.0%
TATACGAAATTTCACAACTG+TGG	+	chr4_4:51235190-51235209	Msa0686670:intron	30.0%
ATGTTAAAAATTGAAGCCCA+AGG	-	chr4_4:51235516-51235535	None:intergenic	30.0%
ATAACGATAAAAAGGTCACT+TGG	+	chr4_4:51235534-51235553	Msa0686670:intron	30.0%
ACATTATAGTATACAGCTGT+AGG	-	chr4_4:51235623-51235642	None:intergenic	30.0%
TTATGCTTAATGAGCAATCA+CGG	-	chr4_4:51235806-51235825	None:intergenic	30.0%
CATTAAGCATAATGTAAGCA+TGG	+	chr4_4:51235814-51235833	Msa0686670:intron	30.0%
ATCTCCATCTTCTACATTTA+TGG	+	chr4_4:51235896-51235915	Msa0686670:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_4	gene	51234558	51244679	51234558	ID=Msa0686670;Name=Msa0686670
chr4_4	mRNA	51234558	51244679	51234558	ID=Msa0686670-mRNA-1;Parent=Msa0686670;Name=Msa0686670-mRNA-1;_AED=0.16;_eAED=0.16;_QI=0\|0.78\|0.86\|1\|0.85\|0.8\|15\|355\|547
chr4_4	exon	51234558	51234684	51234558	ID=Msa0686670-mRNA-1:exon:13195;Parent=Msa0686670-mRNA-1
chr4_4	exon	51235895	51236064	51235895	ID=Msa0686670-mRNA-1:exon:13196;Parent=Msa0686670-mRNA-1
chr4_4	exon	51236982	51237134	51236982	ID=Msa0686670-mRNA-1:exon:13197;Parent=Msa0686670-mRNA-1
chr4_4	exon	51237483	51237672	51237483	ID=Msa0686670-mRNA-1:exon:13198;Parent=Msa0686670-mRNA-1
chr4_4	exon	51237781	51237869	51237781	ID=Msa0686670-mRNA-1:exon:13199;Parent=Msa0686670-mRNA-1
chr4_4	exon	51238534	51238641	51238534	ID=Msa0686670-mRNA-1:exon:13200;Parent=Msa0686670-mRNA-1
chr4_4	exon	51238905	51239003	51238905	ID=Msa0686670-mRNA-1:exon:13201;Parent=Msa0686670-mRNA-1
chr4_4	exon	51239242	51239334	51239242	ID=Msa0686670-mRNA-1:exon:13202;Parent=Msa0686670-mRNA-1
chr4_4	exon	51239422	51239499	51239422	ID=Msa0686670-mRNA-1:exon:13203;Parent=Msa0686670-mRNA-1
chr4_4	exon	51239728	51239832	51239728	ID=Msa0686670-mRNA-1:exon:13204;Parent=Msa0686670-mRNA-1
chr4_4	exon	51241638	51241728	51241638	ID=Msa0686670-mRNA-1:exon:13205;Parent=Msa0686670-mRNA-1
chr4_4	exon	51242087	51242148	51242087	ID=Msa0686670-mRNA-1:exon:13206;Parent=Msa0686670-mRNA-1
chr4_4	exon	51242278	51242457	51242278	ID=Msa0686670-mRNA-1:exon:13207;Parent=Msa0686670-mRNA-1
chr4_4	exon	51243515	51243568	51243515	ID=Msa0686670-mRNA-1:exon:13208;Parent=Msa0686670-mRNA-1
chr4_4	exon	51244280	51244679	51244280	ID=Msa0686670-mRNA-1:exon:13209;Parent=Msa0686670-mRNA-1
chr4_4	CDS	51234558	51234684	51234558	ID=Msa0686670-mRNA-1:cds;Parent=Msa0686670-mRNA-1
chr4_4	CDS	51235895	51236064	51235895	ID=Msa0686670-mRNA-1:cds;Parent=Msa0686670-mRNA-1
chr4_4	CDS	51236982	51237134	51236982	ID=Msa0686670-mRNA-1:cds;Parent=Msa0686670-mRNA-1
chr4_4	CDS	51237483	51237672	51237483	ID=Msa0686670-mRNA-1:cds;Parent=Msa0686670-mRNA-1
chr4_4	CDS	51237781	51237869	51237781	ID=Msa0686670-mRNA-1:cds;Parent=Msa0686670-mRNA-1
chr4_4	CDS	51238534	51238641	51238534	ID=Msa0686670-mRNA-1:cds;Parent=Msa0686670-mRNA-1
chr4_4	CDS	51238905	51239003	51238905	ID=Msa0686670-mRNA-1:cds;Parent=Msa0686670-mRNA-1
chr4_4	CDS	51239242	51239334	51239242	ID=Msa0686670-mRNA-1:cds;Parent=Msa0686670-mRNA-1
chr4_4	CDS	51239422	51239499	51239422	ID=Msa0686670-mRNA-1:cds;Parent=Msa0686670-mRNA-1
chr4_4	CDS	51239728	51239832	51239728	ID=Msa0686670-mRNA-1:cds;Parent=Msa0686670-mRNA-1
chr4_4	CDS	51241638	51241728	51241638	ID=Msa0686670-mRNA-1:cds;Parent=Msa0686670-mRNA-1
chr4_4	CDS	51242087	51242148	51242087	ID=Msa0686670-mRNA-1:cds;Parent=Msa0686670-mRNA-1
chr4_4	CDS	51242278	51242457	51242278	ID=Msa0686670-mRNA-1:cds;Parent=Msa0686670-mRNA-1
chr4_4	CDS	51243515	51243568	51243515	ID=Msa0686670-mRNA-1:cds;Parent=Msa0686670-mRNA-1
chr4_4	CDS	51244280	51244324	51244280	ID=Msa0686670-mRNA-1:cds;Parent=Msa0686670-mRNA-1
chr4_4	three_prime_UTR	51244325	51244679	51244325	ID=Msa0686670-mRNA-1:three_prime_utr;Parent=Msa0686670-mRNA-1

Gene Sequence

>Msa0686670

ATGAGAAACTCATTTAGGACTTTTCACAAACACTTTACTTTCATTTCTTTTCGTAAACTTCCTCACTCTTTTGGTTACGTAACTTCTTCTTCTTCTACGCATCCAACCTTCTCTTTCTCTCAATTTCCATCTCCATCTTCTACATTTATGGAGGTACTCAAAGCAATTGCGAAGCAAGACCTTGCATCCCATGGAAATGTTGCTATCAGAGCTGATCAGAAGAGTTATAGTTACATGCAAATTATTTCTTCGGCGCAGAAGATATCTAATCTCTTATGTGGAAGTAATGTCAAAGCAGGAAATCTTGGTGGAGCTCGAATAGGAATTGTAGCCAAACCCTCTGCTGAATTTGTTGCTGCTGTACTTGGGACTTGGCTTAGTGGTGGTGTCGCTGTTCCACTTGCAGTCAGCTATCCAGAAGTAGAGCTCCTGTATGTGATGAATAATTCGGACACGTCTGCAATACTGAGTACTGAAGATCATAGTGAAGTAATGCAAAACATTGCCAGTAAAACCTCTTCTCAATTTTTCCATCTTCCACCTGTTGCCAATAAGTCGTTAGAGAAGAGCAGAGATGAACATTCACAGAATGGAAAAATTGACGCAGACAGAATTTTCCTGGAAACCATTGAAAGATCAAGTCAAGATCCGGCACTCATTTTGTACACTAGCGGAACAACAGGTAAACCTAAGGGAGTTGTTCATACACACGGAAGCATCATTGCTCAGGTTGAGTTTTTGCCAAAATTTAGTGTCAGCGGAATTTGGAAGAGATGGCGTGAGTCATATCCAACTGAAGGATCTAAGGCCGATGATGCTATAACTGTATTCACTGGAGTTCCAACTATGTATGCCCGATTAATACAAGGTTACCGTGCAATGGATCCTGAGCTACAAGCTGTGTCAGCATCTGCTGCAAGGAACTTGCGTCTAATGATGTGTGGGTCCTCTGCACTTCCTCAACCTGTTATGCAAGAATGGGAAGCTATCACTGGCCATCGTTTATTGGAGCGATATGGCATGACGGAATTTGTCATGGCATTGTCAAATCCATTAAAAGGTGAGCGTAAGGCAGGCACAGTTGGAAAACCATTACCCGGAGTGCAGATCAAGATTCTTGCAGATGAGGAGCGTGGGAATGAAAAAAATGAAGCCGGCGAGCTTTGCGTTAAAAGCCCATCATTGTTTAAAGAATATTGGAAACTTCCAGAGGTAACTAAGGAATCATTTACGGAAGAAGGTTTCTTTAAGACCGGAGATGCAGTTACAACAGATAAAGATGGATATTACATCATTCTAGGACGTACTAGTGCGGACATCATCAAGGCCGGTGGCTATAAGCTGTCTGCATTAGAAATCGAATCACATCCAGCTGTCTCAGAATGTTGTGTCTTGGGCTTACCACACAAAGAATATGGAGAAATTGTTGGTGCAATTATCGTGCCAGAAGCTGATGTTAAACGAAAGCGAGATGAAGAGTCAAAGCCGGCTCTAAGCCTAGAAGAGTTATCCACTTGGGCCAAAGATAAACTTGCACCTTACAAGATACCAACTCGTCTGATCCTTTGGGACTCACTCCCTCGCAATGCAATGGGAAAGGTAAGTAAAAAAGAGCTGAAGAAGATCCTAGCTTCAGAACAGTAA

Protein sequence

>Msa0686670

MRNSFRTFHKHFTFISFRKLPHSFGYVTSSSSTHPTFSFSQFPSPSSTFMEVLKAIAKQDLASHGNVAIRADQKSYSYMQIISSAQKISNLLCGSNVKAGNLGGARIGIVAKPSAEFVAAVLGTWLSGGVAVPLAVSYPEVELLYVMNNSDTSAILSTEDHSEVMQNIASKTSSQFFHLPPVANKSLEKSRDEHSQNGKIDADRIFLETIERSSQDPALILYTSGTTGKPKGVVHTHGSIIAQVEFLPKFSVSGIWKRWRESYPTEGSKADDAITVFTGVPTMYARLIQGYRAMDPELQAVSASAARNLRLMMCGSSALPQPVMQEWEAITGHRLLERYGMTEFVMALSNPLKGERKAGTVGKPLPGVQIKILADEERGNEKNEAGELCVKSPSLFKEYWKLPEVTKESFTEEGFFKTGDAVTTDKDGYYIILGRTSADIIKAGGYKLSALEIESHPAVSECCVLGLPHKEYGEIVGAIIVPEADVKRKRDEESKPALSLEELSTWAKDKLAPYKIPTRLILWDSLPRNAMGKVSKKELKKILASEQ*