AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0829210

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0829210 MtrunA17_Chr3g0137521 98.400 125 2 0 1 125 1 125 5.96e-79 234
Msa0829210 MtrunA17_Chr3g0137521 74.615 130 31 2 127 255 1 129 1.81e-54 172
Msa0829210 MtrunA17_Chr3g0137531 95.420 131 3 2 127 256 1 129 5.08e-62 191
Msa0829210 MtrunA17_Chr3g0137531 72.441 127 30 2 1 125 1 124 3.16e-42 140
Msa0829210 MtrunA17_Chr1g0149481 56.627 166 34 4 127 256 1 164 6.49e-48 156
Msa0829210 MtrunA17_Chr1g0149481 57.862 159 31 3 1 124 1 158 3.12e-47 154
Msa0829210 MtrunA17_Chr1g0149491 60.784 153 35 3 127 256 1 151 3.45e-43 144
Msa0829210 MtrunA17_Chr1g0149491 59.589 146 36 2 1 124 1 145 9.83e-42 140
Msa0829210 MtrunA17_Chr1g0149471 79.762 84 16 1 173 256 128 210 1.48e-39 136
Msa0829210 MtrunA17_Chr1g0149471 78.481 79 16 1 46 124 127 204 1.27e-34 123
Msa0829210 MtrunA17_Chr4g0060391 50.820 122 56 2 135 256 6 123 2.74e-31 112
Msa0829210 MtrunA17_Chr4g0060391 52.137 117 51 2 8 124 6 117 8.91e-31 110
Msa0829210 MtrunA17_Chr2g0290521 61.538 104 37 3 21 122 18 120 4.34e-31 112
Msa0829210 MtrunA17_Chr2g0290521 47.619 126 61 3 130 255 6 126 8.24e-27 100
Msa0829210 MtrunA17_Chr4g0056371 51.639 122 53 3 8 124 6 126 1.26e-29 108
Msa0829210 MtrunA17_Chr4g0056371 63.095 84 30 1 173 256 50 132 2.24e-29 107
Msa0829210 MtrunA17_Chr4g0056411 40.588 170 58 6 127 255 1 168 2.49e-27 103
Msa0829210 MtrunA17_Chr4g0056411 64.198 81 27 2 45 124 84 163 5.45e-26 100
Msa0829210 MtrunA17_Chr4g0056391 56.818 88 36 2 170 256 57 143 5.97e-27 101
Msa0829210 MtrunA17_Chr4g0056391 63.415 82 28 2 44 124 57 137 6.23e-27 101
Msa0829210 MtrunA17_Chr4g0056401 57.647 85 34 2 173 256 51 134 7.24e-26 98.6
Msa0829210 MtrunA17_Chr4g0056401 60.000 80 30 2 46 124 50 128 3.64e-25 96.7
Msa0829210 MtrunA17_Chr5g0399081 47.619 84 41 2 173 255 95 176 2.78e-21 87.8
Msa0829210 MtrunA17_Chr5g0399081 43.902 82 43 2 44 124 92 171 2.93e-18 80.1
Msa0829210 MtrunA17_Chr4g0056381 57.143 84 33 3 173 255 105 186 6.77e-21 87.0
Msa0829210 MtrunA17_Chr4g0056381 57.500 80 31 3 47 125 105 182 7.16e-19 81.6
Msa0829210 MtrunA17_Chr5g0399091 46.429 84 42 2 173 255 88 169 1.19e-20 86.3
Msa0829210 MtrunA17_Chr5g0399091 42.683 82 44 2 44 124 85 164 1.11e-17 78.2
Msa0829210 MtrunA17_Chr2g0290571 48.750 80 38 2 176 255 122 198 2.63e-15 72.4
Msa0829210 MtrunA17_Chr2g0290571 45.333 75 38 2 50 124 122 193 4.53e-13 66.2
Msa0829210 MtrunA17_Chr3g0120411 46.512 86 45 1 170 255 251 335 2.20e-14 71.6
Msa0829210 MtrunA17_Chr3g0120411 43.210 81 45 1 44 124 251 330 7.86e-11 61.2
Msa0829210 MtrunA17_Chr2g0290561 46.250 80 40 1 176 255 118 194 4.89e-14 68.9
Msa0829210 MtrunA17_Chr2g0290561 43.421 76 40 1 50 125 118 190 2.70e-13 66.6
Msa0829210 MtrunA17_Chr2g0290531 41.772 79 40 3 180 256 186 260 3.42e-11 62.0
Msa0829210 MtrunA17_Chr2g0290531 47.945 73 32 3 54 124 186 254 3.88e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0829210 AT2G45180.1 69.048 84 25 1 173 256 52 134 3.10e-32 115
Msa0829210 AT2G45180.1 74.074 81 20 1 44 124 49 128 1.52e-31 113
Msa0829210 AT4G12490.1 60.000 95 36 2 163 256 89 182 5.04e-30 111
Msa0829210 AT4G12490.1 41.243 177 50 4 1 124 1 176 1.62e-27 104
Msa0829210 AT4G12500.1 38.764 178 60 4 127 256 1 177 1.38e-29 110
Msa0829210 AT4G12500.1 64.634 82 27 2 44 124 91 171 5.77e-27 103
Msa0829210 AT4G12480.1 60.920 87 32 2 171 256 83 168 4.57e-29 108
Msa0829210 AT4G12480.1 63.415 82 28 2 44 124 82 162 2.68e-26 101
Msa0829210 AT4G12470.1 58.889 90 35 2 168 256 73 161 5.09e-29 108
Msa0829210 AT4G12470.1 60.000 85 32 2 42 125 73 156 6.37e-26 100
Msa0829210 AT5G46900.1 64.835 91 28 3 166 256 41 127 3.03e-28 105
Msa0829210 AT5G46900.1 70.238 84 21 3 41 124 42 121 2.37e-26 100
Msa0829210 AT1G62510.1 61.176 85 31 2 172 255 66 149 1.61e-27 103
Msa0829210 AT1G62510.1 67.500 80 24 2 46 124 66 144 2.57e-27 103
Msa0829210 AT5G46890.1 51.587 126 52 5 135 256 7 127 7.59e-27 101
Msa0829210 AT5G46890.1 54.762 126 50 5 1 124 1 121 3.71e-26 99.8
Msa0829210 AT4G00165.1 54.545 99 41 3 29 124 26 123 8.87e-27 101
Msa0829210 AT4G00165.1 47.664 107 51 3 153 255 23 128 2.40e-24 94.7
Msa0829210 AT4G00165.2 54.545 99 41 3 29 124 26 123 8.87e-27 101
Msa0829210 AT4G00165.2 47.664 107 51 3 153 255 23 128 2.40e-24 94.7
Msa0829210 AT1G12090.1 62.195 82 29 2 175 255 57 137 1.58e-26 100
Msa0829210 AT1G12090.1 66.667 78 24 2 49 125 57 133 9.86e-26 98.6
Msa0829210 AT4G12520.1 52.893 121 53 3 137 256 12 129 2.33e-26 100
Msa0829210 AT4G12520.1 53.968 126 53 5 1 124 1 123 9.89e-26 98.6
Msa0829210 AT4G12510.1 52.893 121 53 3 137 256 12 129 2.33e-26 100
Msa0829210 AT4G12510.1 53.968 126 53 5 1 124 1 123 9.89e-26 98.6
Msa0829210 AT4G22460.1 54.639 97 44 0 25 121 28 124 8.47e-26 99.0
Msa0829210 AT4G22460.1 48.571 105 49 2 154 254 28 131 2.44e-23 92.4
Msa0829210 AT1G12100.1 44.628 121 61 4 7 124 9 126 2.14e-25 97.8
Msa0829210 AT1G12100.1 41.129 124 70 3 135 256 10 132 4.60e-24 94.4
Msa0829210 AT4G12550.1 50.000 92 44 2 166 256 21 111 2.63e-24 94.4
Msa0829210 AT4G12550.1 56.790 81 33 2 45 124 26 105 8.20e-24 92.8
Msa0829210 AT4G12530.1 50.000 86 42 1 170 255 31 115 1.87e-21 87.0
Msa0829210 AT4G12530.1 45.679 81 43 1 44 124 31 110 5.14e-17 75.1
Msa0829210 AT4G12545.1 50.000 92 41 3 166 256 21 108 2.65e-21 86.3
Msa0829210 AT4G12545.1 56.790 81 30 3 45 124 26 102 1.56e-20 84.3
Msa0829210 AT3G22142.1 52.381 84 37 3 173 255 1397 1478 2.90e-19 87.4
Msa0829210 AT3G22142.1 56.944 72 27 3 47 116 1397 1466 5.30e-17 80.9
Msa0829210 AT2G10940.2 55.422 83 34 3 44 125 205 285 1.77e-17 80.5
Msa0829210 AT2G10940.2 47.727 88 43 3 170 256 205 290 2.89e-17 79.7
Msa0829210 AT2G10940.1 55.422 83 34 3 44 125 205 285 1.77e-17 80.5
Msa0829210 AT2G10940.1 47.727 88 43 3 170 256 205 290 2.89e-17 79.7
Msa0829210 AT3G22120.2 51.190 84 39 2 173 255 267 349 2.03e-17 80.9
Msa0829210 AT3G22120.2 49.367 79 38 2 47 124 267 344 1.93e-14 72.4
Msa0829210 AT3G22120.1 51.190 84 39 2 173 255 250 332 3.80e-17 80.1
Msa0829210 AT3G22120.1 49.367 79 38 2 47 124 250 327 3.05e-14 71.6
Msa0829210 AT4G15160.2 49.412 85 40 3 173 255 99 182 1.14e-15 73.6
Msa0829210 AT4G15160.2 48.750 80 39 2 47 125 99 177 7.57e-15 71.2
Msa0829210 AT4G15160.1 49.412 85 40 3 173 255 181 264 2.72e-15 73.9
Msa0829210 AT4G15160.1 48.750 80 39 2 47 125 181 259 2.14e-14 71.6
Msa0829210 AT4G22490.1 38.043 92 53 3 165 255 28 116 9.42e-14 66.6
Msa0829210 AT4G22490.1 31.818 88 57 2 37 124 27 111 8.25e-11 58.5
Msa0829210 AT1G62500.1 42.529 87 47 3 170 255 209 293 1.46e-13 69.3
Msa0829210 AT1G62500.1 43.056 72 39 2 47 117 212 282 9.41e-12 64.3
Msa0829210 AT4G22470.1 22.749 211 153 4 45 255 174 374 5.33e-12 65.5

Find 82 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTAACTTTAAGGTATCTT+TGG 0.166346 5_4:-2139043 None:intergenic
TAAATGTTGTGCATTGATTA+AGG 0.202840 5_4:+2136198 Msa0829210:CDS
AGCACAAACCCCTAACTTTA+AGG 0.205248 5_4:-2139052 None:intergenic
ATCTTTGGGGCAGTGACCTT+GGG 0.214560 5_4:-2139028 None:intergenic
AGCACAAACCCCTAGCTTTA+AGG 0.218235 5_4:-2136125 None:intergenic
TTGTCTTTGCACTGCAATTA+AGG 0.231170 5_4:+2139173 Msa0829210:CDS
AATCAAAGGTTTGGCTGATT+TGG 0.237754 5_4:+2139140 Msa0829210:CDS
GGGTTGGTTAATGTTGTTAT+TGG 0.240421 5_4:+2136157 Msa0829210:CDS
TCCTTAGGGCATTGACCTTT+GGG 0.253417 5_4:-2136100 None:intergenic
ATCCTTAGGGCATTGACCTT+TGG 0.263717 5_4:-2136101 None:intergenic
TAAAATGGTGGCAAAGAATT+TGG 0.290687 5_4:-2138914 None:intergenic
CCTAACTTTAAGGTATCTTT+GGG 0.293316 5_4:-2139042 None:intergenic
GAAGGTGTTGGCTTTGGCTT+TGG 0.296299 5_4:-2139003 None:intergenic
GATTAAGGGTTTGGCTGATT+TGG 0.309202 5_4:+2136213 Msa0829210:CDS
AAGAGAAAGCACTAAAATTA+AGG 0.315935 5_4:-2135996 None:intergenic
TGGGGTGAAGGTGTTGGCTT+TGG 0.321997 5_4:-2139009 None:intergenic
CCCAAAGATACCTTAAAGTT+AGG 0.331524 5_4:+2139042 Msa0829210:CDS
GTTGGCTTTGGCTTTGGCTT+TGG 0.335137 5_4:-2138997 None:intergenic
TTGTCTTTGCACTGCACTTA+AGG 0.338630 5_4:+2136246 Msa0829210:CDS
CCTAGCTTTAAGGTATCCTT+AGG 0.341849 5_4:-2136115 None:intergenic
AAATGTTGTGCATTGATTAA+GGG 0.358491 5_4:+2136199 Msa0829210:CDS
TATCTTTGGGGCAGTGACCT+TGG 0.380498 5_4:-2139029 None:intergenic
CCAAAGATACCTTAAAGTTA+GGG 0.385359 5_4:+2139043 Msa0829210:CDS
AGGATCCAGGTGAGGGTGAT+AGG 0.388310 5_4:-2139225 None:intergenic
GGGCAGTGACCTTGGGGTGA+AGG 0.389809 5_4:-2139021 None:intergenic
AAGGAGAGAGAGTACTAAAA+TGG 0.390388 5_4:-2138929 None:intergenic
ATCACCCTCACCTGGATCCT+TGG 0.406696 5_4:+2139228 Msa0829210:CDS
GTTGTTGGGAATCCTGCTTC+AGG 0.413980 5_4:+2139099 Msa0829210:CDS
GAAGGTGTTGGCTTTGGTGA+TGG 0.414445 5_4:-2136055 None:intergenic
TTTGGCTTTGGCTTTGGTGA+TGG 0.416452 5_4:-2138991 None:intergenic
CTCAACATTTACTGAGGCTA+AGG 0.420857 5_4:+2138957 Msa0829210:CDS
CCTTTGGGTGAAGGTGGAAC+AGG 0.427685 5_4:-2136085 None:intergenic
GTTATTGGAAACCCTCCTTC+AGG 0.428430 5_4:+2136172 Msa0829210:CDS
TTGTGCATTGATTAAGGGTT+TGG 0.429971 5_4:+2136204 Msa0829210:CDS
AGGAGCACGCTGAGCGTGAT+AGG 0.432511 5_4:-2136298 None:intergenic
GGGCATTGACCTTTGGGTGA+AGG 0.436950 5_4:-2136094 None:intergenic
AATTAAGGCAGAGAATTTGT+TGG 0.438270 5_4:-2135981 None:intergenic
TTGACAAGCACCAAGGATCC+AGG 0.457767 5_4:-2139238 None:intergenic
GCAGGTGAAGGTGTTGGCTT+TGG 0.458002 5_4:-2136061 None:intergenic
GACTGGTTAATGTTGTTGTT+GGG 0.463209 5_4:+2139085 Msa0829210:CDS
TAAGGGTTTGGCTGATTTGG+AGG 0.464987 5_4:+2136216 Msa0829210:CDS
TTGTGCAATAATCAAAGGTT+TGG 0.466120 5_4:+2139131 Msa0829210:CDS
GGACTGGTTAATGTTGTTGT+TGG 0.480624 5_4:+2139084 Msa0829210:CDS
GGTGAAGGTGGAACAGGAGC+AGG 0.481606 5_4:-2136079 None:intergenic
GCACTTAAGGCTAATGTGCT+TGG 0.487972 5_4:+2136259 Msa0829210:CDS
TGACCTTGGGGTGAAGGTGT+TGG 0.502306 5_4:-2139015 None:intergenic
CCTAAGGATACCTTAAAGCT+AGG 0.505679 5_4:+2136115 Msa0829210:CDS
CTAAGGATACCTTAAAGCTA+GGG 0.512185 5_4:+2136116 Msa0829210:CDS
GGTGTTGGCTTTGGTGATGG+TGG 0.522065 5_4:-2136052 None:intergenic
AATTGTTGTGCAATAATCAA+AGG 0.523157 5_4:+2139126 Msa0829210:CDS
GCAATTAAGGCCAATGTGCT+CGG 0.533371 5_4:+2139186 Msa0829210:CDS
CATTGACCTTTGGGTGAAGG+TGG 0.538821 5_4:-2136091 None:intergenic
TCTTTGGGGCAGTGACCTTG+GGG 0.545751 5_4:-2139027 None:intergenic
CCTTAGGCACATTGAAAACC+AGG 0.555069 5_4:-2139273 None:intergenic
CAAAGATACCTTAAAGTTAG+GGG 0.560461 5_4:+2139044 Msa0829210:CDS
ACATTGGAAACCAGGAGGGA+CGG 0.567925 5_4:-2136338 None:intergenic
CCTGTTCCACCTTCACCCAA+AGG 0.572122 5_4:+2136085 Msa0829210:CDS
CTAGCTTTAAGGTATCCTTA+GGG 0.574702 5_4:-2136114 None:intergenic
AACATTTACTACCTGAAGGA+GGG 0.575828 5_4:-2136183 None:intergenic
ACAGGAGCAGGTGAAGGTGT+TGG 0.576951 5_4:-2136067 None:intergenic
TGATGGTGGACAATTAGCTT+CGG 0.580942 5_4:-2136038 None:intergenic
ACCCAAAGGTCAATGCCCTA+AGG 0.586732 5_4:+2136099 Msa0829210:CDS
ATGTACCTATCACCCTCACC+TGG 0.591606 5_4:+2139220 Msa0829210:CDS
CAGTAAATGTTGAGTAAGCA+AGG 0.592270 5_4:-2138948 None:intergenic
GGTGGAACAGGAGCAGGTGA+AGG 0.595926 5_4:-2136073 None:intergenic
AGCTTCGGTGAATGTTGAGT+AGG 0.595958 5_4:-2136023 None:intergenic
TCAACATTTACTGAGGCTAA+GGG 0.596026 5_4:+2138958 Msa0829210:CDS
CAAGTTTATTCCGAGCACAT+TGG 0.596849 5_4:-2139196 None:intergenic
GGTGGCAAAGAATTTGGTGT+TGG 0.599992 5_4:-2138908 None:intergenic
CAACAATTACTACCTGAAGC+AGG 0.602543 5_4:-2139111 None:intergenic
CAACATTTACTACCTGAAGG+AGG 0.605436 5_4:-2136184 None:intergenic
AGCACCAAGGATCCAGGTGA+GGG 0.612333 5_4:-2139232 None:intergenic
AAGCCAACACCTTCACCCCA+AGG 0.614009 5_4:+2139012 Msa0829210:CDS
TGCTTACTCAACATTTACTG+AGG 0.622636 5_4:+2138951 Msa0829210:CDS
GCACAACATTTACTACCTGA+AGG 0.630292 5_4:-2136187 None:intergenic
GAGAGAGAGTACTAAAATGG+TGG 0.630559 5_4:-2138926 None:intergenic
AAGCACCAAGGATCCAGGTG+AGG 0.645068 5_4:-2139233 None:intergenic
TAAGGATACCTTAAAGCTAG+GGG 0.646982 5_4:+2136117 Msa0829210:CDS
AGGGACATTGGAAACCAGGA+GGG 0.669232 5_4:-2136342 None:intergenic
AGGCACATTGAAAACCAGGA+GGG 0.674973 5_4:-2139269 None:intergenic
CTAACTTTAAGGTATCTTTG+GGG 0.708820 5_4:-2139041 None:intergenic
TAGGCACATTGAAAACCAGG+AGG 0.727521 5_4:-2139270 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TACGTTTTATTAATTTATAT+GGG - chr5_4:2136535-2136554 None:intergenic 10.0%
!! AAATGAATATAAGATAATTT+AGG - chr5_4:2136741-2136760 None:intergenic 10.0%
!!! TGATTTTTAATTAATTGATT+TGG + chr5_4:2137709-2137728 Msa0829210:intron 10.0%
!!! TTTTCGTTTTTTTTTTTTTT+TGG - chr5_4:2137997-2138016 None:intergenic 10.0%
!!! TGTAACATTTTTTATGTTAT+CGG + chr5_4:2136476-2136495 Msa0829210:intron 15.0%
!!! GTACGTTTTATTAATTTATA+TGG - chr5_4:2136536-2136555 None:intergenic 15.0%
!!! GCAAAACTTTTTTTTATTAT+TGG - chr5_4:2136570-2136589 None:intergenic 15.0%
!!! AATAAAAAAAAGTTTTGCAA+AGG + chr5_4:2136572-2136591 Msa0829210:intron 15.0%
!!! GTTTTTTTTGCTTATAATTT+GGG - chr5_4:2136845-2136864 None:intergenic 15.0%
!!! AGTTTTTTTTGCTTATAATT+TGG - chr5_4:2136846-2136865 None:intergenic 15.0%
!!! AACTTTTTTGCTTATAATAT+GGG - chr5_4:2136881-2136900 None:intergenic 15.0%
!!! AAACTTTTTTGCTTATAATA+TGG - chr5_4:2136882-2136901 None:intergenic 15.0%
!!! TTTTTTCTTATAATTTAGGA+TGG + chr5_4:2137120-2137139 Msa0829210:intron 15.0%
!!! CTTAATTATTTTGCTTTTTT+TGG - chr5_4:2137260-2137279 None:intergenic 15.0%
!! CAAAAAAAGCAAAATAATTA+AGG + chr5_4:2137258-2137277 Msa0829210:intron 15.0%
!! TAAAATAATAGATAGTTATG+TGG + chr5_4:2137289-2137308 Msa0829210:intron 15.0%
!!! ATTTTTAGAAATAGATGTTA+TGG - chr5_4:2137555-2137574 None:intergenic 15.0%
!!! ATTTGGTAAAATATTGATTT+TGG + chr5_4:2137726-2137745 Msa0829210:intron 15.0%
!! TTAAGTATAAAAATCATGTT+TGG + chr5_4:2137819-2137838 Msa0829210:intron 15.0%
!!! TTTTTTTTTTTTTGGTTACA+AGG - chr5_4:2137989-2138008 None:intergenic 15.0%
!!! AAAAATGAGTTGTAATATTT+TGG - chr5_4:2138704-2138723 None:intergenic 15.0%
!!! TTTTGGTCAAACTAATAATT+TGG + chr5_4:2136703-2136722 Msa0829210:intron 20.0%
!! GATGTAAAAACAATTTGTAT+TGG - chr5_4:2137017-2137036 None:intergenic 20.0%
!! TTTCTATGAAACTTATTTCA+AGG + chr5_4:2137037-2137056 Msa0829210:intron 20.0%
!! ATGAACTTCTAATGATAAAA+CGG - chr5_4:2138365-2138384 None:intergenic 20.0%
!! ATATCATCTCATAGATAAAA+TGG + chr5_4:2138626-2138645 Msa0829210:intron 20.0%
!! AGATAATAACAAATGAGAAA+AGG + chr5_4:2138659-2138678 Msa0829210:intron 20.0%
!!! ATTGCTACATTTAATTTGTA+AGG + chr5_4:2138866-2138885 Msa0829210:intron 20.0%
!!! AAGAGAAAGCACTAAAATTA+AGG - chr5_4:2135999-2136018 None:intergenic 25.0%
!!! TAAATGTTGTGCATTGATTA+AGG + chr5_4:2136198-2136217 Msa0829210:CDS 25.0%
!!! AAATGTTGTGCATTGATTAA+GGG + chr5_4:2136199-2136218 Msa0829210:CDS 25.0%
!! AAAAAAGTTTTGCAAAGGAA+TGG + chr5_4:2136577-2136596 Msa0829210:intron 25.0%
!! TCAAAAAATCATCTTTTCCA+AGG + chr5_4:2136930-2136949 Msa0829210:intron 25.0%
!! AATTGCATTTAATTTCTCCT+TGG - chr5_4:2136950-2136969 None:intergenic 25.0%
!! TTTCTTATAATTTAGGATGG+AGG + chr5_4:2137123-2137142 Msa0829210:intron 25.0%
!! TTCTTATAATTTAGGATGGA+GGG + chr5_4:2137124-2137143 Msa0829210:intron 25.0%
!! TTATGTGGTCTTTCTTTTAA+GGG + chr5_4:2137304-2137323 Msa0829210:intron 25.0%
! TTATTAAAAAGCTTCTGAGT+TGG - chr5_4:2137395-2137414 None:intergenic 25.0%
! AGTTTCCCATATCAAATTTA+AGG + chr5_4:2137477-2137496 Msa0829210:intron 25.0%
! GTTTCCCATATCAAATTTAA+GGG + chr5_4:2137478-2137497 Msa0829210:intron 25.0%
! CATACCCTTAAATTTGATAT+GGG - chr5_4:2137485-2137504 None:intergenic 25.0%
! ACATACCCTTAAATTTGATA+TGG - chr5_4:2137486-2137505 None:intergenic 25.0%
!! TCAAATTTAAGGGTATGTTT+AGG + chr5_4:2137488-2137507 Msa0829210:intron 25.0%
! TTTAGGTAAGTTTGATTGAT+TGG - chr5_4:2137610-2137629 None:intergenic 25.0%
!!! TCCTCTTTTAAAATTGATTG+CGG - chr5_4:2137634-2137653 None:intergenic 25.0%
! AAAGTTAGTTAAAAGTGAAC+TGG + chr5_4:2137752-2137771 Msa0829210:intron 25.0%
!!! AGTATATTTTAATTTCACCG+TGG - chr5_4:2138044-2138063 None:intergenic 25.0%
!! ATTGCATGATTTTGTATGTT+TGG - chr5_4:2138160-2138179 None:intergenic 25.0%
! TTTGAATTTGAATCCTTAGA+GGG - chr5_4:2138278-2138297 None:intergenic 25.0%
!!! AAGCCAATACAATTGATTTT+TGG + chr5_4:2138497-2138516 Msa0829210:intron 25.0%
!!! AGCCAATACAATTGATTTTT+GGG + chr5_4:2138498-2138517 Msa0829210:intron 25.0%
!!! ATTTGTGTTGCCTTTAAAAT+AGG + chr5_4:2138811-2138830 Msa0829210:intron 25.0%
!!! AATGAGTGAACCTATTTTAA+AGG - chr5_4:2138824-2138843 None:intergenic 25.0%
! AATTGTTGTGCAATAATCAA+AGG + chr5_4:2139126-2139145 Msa0829210:CDS 25.0%
AATTAAGGCAGAGAATTTGT+TGG - chr5_4:2135984-2136003 None:intergenic 30.0%
TAAAACAATGACAATGACAG+TGG - chr5_4:2136624-2136643 None:intergenic 30.0%
! GTAGATTTCTTAACGTGTTT+TGG + chr5_4:2136686-2136705 Msa0829210:intron 30.0%
TTTGCTTATAATATGGGACA+AGG - chr5_4:2136875-2136894 None:intergenic 30.0%
! GTTATGTGGTCTTTCTTTTA+AGG + chr5_4:2137303-2137322 Msa0829210:intron 30.0%
!! ATGTTTAGGTTGATGGTATA+AGG + chr5_4:2137502-2137521 Msa0829210:intron 30.0%
TTAAAATTGATTGCGGCTTT+AGG - chr5_4:2137627-2137646 None:intergenic 30.0%
AAAAGTGAAATGTGTAGCTT+TGG - chr5_4:2137848-2137867 None:intergenic 30.0%
!! AAGTTGTAGTTTGTAGCTTT+TGG + chr5_4:2138082-2138101 Msa0829210:intron 30.0%
!! AGTTGTAGTTTGTAGCTTTT+GGG + chr5_4:2138083-2138102 Msa0829210:intron 30.0%
!! TTTTGGGAAAACACTGAAAA+TGG + chr5_4:2138099-2138118 Msa0829210:intron 30.0%
TTCCGCATGAGAAAAAAATA+TGG + chr5_4:2138181-2138200 Msa0829210:intron 30.0%
TCCGCATGAGAAAAAAATAT+GGG + chr5_4:2138182-2138201 Msa0829210:intron 30.0%
!!! TCCCATATTTTTTTCTCATG+CGG - chr5_4:2138186-2138205 None:intergenic 30.0%
GTTTGAATTTGAATCCTTAG+AGG - chr5_4:2138279-2138298 None:intergenic 30.0%
!!! AGTTTTGGATTTGAAAGTCA+CGG - chr5_4:2138340-2138359 None:intergenic 30.0%
! AATGATAAAACGGCAAGTTT+TGG - chr5_4:2138355-2138374 None:intergenic 30.0%
!! ATTGTATTGGCTTGATCAAA+CGG - chr5_4:2138490-2138509 None:intergenic 30.0%
CACCCAAAAATCAATTGTAT+TGG - chr5_4:2138503-2138522 None:intergenic 30.0%
CTGTAAAAGAACATGTTTGT+TGG - chr5_4:2138532-2138551 None:intergenic 30.0%
TCATCTCATAGATAAAATGG+TGG + chr5_4:2138629-2138648 Msa0829210:intron 30.0%
CATCTCATAGATAAAATGGT+GGG + chr5_4:2138630-2138649 Msa0829210:intron 30.0%
TTCCACTCAATTAGATTTCT+AGG + chr5_4:2138776-2138795 Msa0829210:intron 30.0%
AACCTAGAAATCTAATTGAG+TGG - chr5_4:2138781-2138800 None:intergenic 30.0%
TAAAGGCAACACAAATTGTA+AGG - chr5_4:2138807-2138826 None:intergenic 30.0%
AAATGTAGCAATATGTGTGT+TGG - chr5_4:2138858-2138877 None:intergenic 30.0%
TGTAAGGTAAACTATAGTCA+TGG + chr5_4:2138882-2138901 Msa0829210:intron 30.0%
TAAAATGGTGGCAAAGAATT+TGG - chr5_4:2138917-2138936 None:intergenic 30.0%
!! CTAACTTTAAGGTATCTTTG+GGG - chr5_4:2139044-2139063 None:intergenic 30.0%
!! CCTAACTTTAAGGTATCTTT+GGG - chr5_4:2139045-2139064 None:intergenic 30.0%
CCAAAGATACCTTAAAGTTA+GGG + chr5_4:2139043-2139062 Msa0829210:CDS 30.0%
CAAAGATACCTTAAAGTTAG+GGG + chr5_4:2139044-2139063 Msa0829210:CDS 30.0%
TTGTGCAATAATCAAAGGTT+TGG + chr5_4:2139131-2139150 Msa0829210:CDS 30.0%
!! CTAGCTTTAAGGTATCCTTA+GGG - chr5_4:2136117-2136136 None:intergenic 35.0%
CTAAGGATACCTTAAAGCTA+GGG + chr5_4:2136116-2136135 Msa0829210:CDS 35.0%
TAAGGATACCTTAAAGCTAG+GGG + chr5_4:2136117-2136136 Msa0829210:CDS 35.0%
!! GGGTTGGTTAATGTTGTTAT+TGG + chr5_4:2136157-2136176 Msa0829210:CDS 35.0%
AACATTTACTACCTGAAGGA+GGG - chr5_4:2136186-2136205 None:intergenic 35.0%
!! TTGTGCATTGATTAAGGGTT+TGG + chr5_4:2136204-2136223 Msa0829210:CDS 35.0%
CAACCTAATTGGAATCCTTA+GGG - chr5_4:2136364-2136383 None:intergenic 35.0%
ACAACCTAATTGGAATCCTT+AGG - chr5_4:2136365-2136384 None:intergenic 35.0%
ACAATGAGACACAACCTAAT+TGG - chr5_4:2136375-2136394 None:intergenic 35.0%
! TGCTTATAATTTGGGACAGA+GGG - chr5_4:2136837-2136856 None:intergenic 35.0%
! TTGCTTATAATTTGGGACAG+AGG - chr5_4:2136838-2136857 None:intergenic 35.0%
! TCCTGTTTTACTAAGTAGCT+TGG + chr5_4:2137196-2137215 Msa0829210:intron 35.0%
TCCAAGCTACTTAGTAAAAC+AGG - chr5_4:2137200-2137219 None:intergenic 35.0%
! AAAAAGCTTCTGAGTTGGAT+TGG - chr5_4:2137390-2137409 None:intergenic 35.0%
!! TAAGGGTATGTTTAGGTTGA+TGG + chr5_4:2137495-2137514 Msa0829210:intron 35.0%
!! TTTAGGTTGATGGTATAAGG+TGG + chr5_4:2137505-2137524 Msa0829210:intron 35.0%
GAAATAGAGCTGAGTGAAAT+AGG - chr5_4:2137586-2137605 None:intergenic 35.0%
!! GCCGCAATCAATTTTAAAAG+AGG + chr5_4:2137630-2137649 Msa0829210:intron 35.0%
AAAGTGAACTGGACTATGTT+TGG + chr5_4:2137763-2137782 Msa0829210:intron 35.0%
TGGAAACAAACATGCATCTA+AGG - chr5_4:2138024-2138043 None:intergenic 35.0%
TAGATGCATGTTTGTTTCCA+CGG + chr5_4:2138024-2138043 Msa0829210:intron 35.0%
GAAAACACTGAAAATGGTTG+TGG + chr5_4:2138105-2138124 Msa0829210:intron 35.0%
!! ATATGGGACATCGATTTCTA+TGG + chr5_4:2138198-2138217 Msa0829210:intron 35.0%
AATGCAAAGAAAACCCTCTA+AGG + chr5_4:2138262-2138281 Msa0829210:intron 35.0%
! AGAAGTTTTCTCCACTTTCA+TGG - chr5_4:2138308-2138327 None:intergenic 35.0%
!! TCATAGATAAAATGGTGGGT+GGG + chr5_4:2138634-2138653 Msa0829210:intron 35.0%
! TTTGTGATAACGTTTCCAGT+CGG - chr5_4:2138747-2138766 None:intergenic 35.0%
!! AAGGAGAGAGAGTACTAAAA+TGG - chr5_4:2138932-2138951 None:intergenic 35.0%
CAGTAAATGTTGAGTAAGCA+AGG - chr5_4:2138951-2138970 None:intergenic 35.0%
TGCTTACTCAACATTTACTG+AGG + chr5_4:2138951-2138970 Msa0829210:CDS 35.0%
TCAACATTTACTGAGGCTAA+GGG + chr5_4:2138958-2138977 Msa0829210:CDS 35.0%
CCCAAAGATACCTTAAAGTT+AGG + chr5_4:2139042-2139061 Msa0829210:CDS 35.0%
!! CCCTAACTTTAAGGTATCTT+TGG - chr5_4:2139046-2139065 None:intergenic 35.0%
! GACTGGTTAATGTTGTTGTT+GGG + chr5_4:2139085-2139104 Msa0829210:CDS 35.0%
AATCAAAGGTTTGGCTGATT+TGG + chr5_4:2139140-2139159 Msa0829210:CDS 35.0%
TTGTCTTTGCACTGCAATTA+AGG + chr5_4:2139173-2139192 Msa0829210:CDS 35.0%
!!! TTGTTTTTTGACAAGCACCA+AGG - chr5_4:2139248-2139267 None:intergenic 35.0%
!! TGATGGTGGACAATTAGCTT+CGG - chr5_4:2136041-2136060 None:intergenic 40.0%
!! CCTAGCTTTAAGGTATCCTT+AGG - chr5_4:2136118-2136137 None:intergenic 40.0%
CCTAAGGATACCTTAAAGCT+AGG + chr5_4:2136115-2136134 Msa0829210:CDS 40.0%
!! GGGTTTGTGCTGATCTTTTA+GGG + chr5_4:2136137-2136156 Msa0829210:CDS 40.0%
!!! TTGTGCTGATCTTTTAGGGT+TGG + chr5_4:2136141-2136160 Msa0829210:CDS 40.0%
CAACATTTACTACCTGAAGG+AGG - chr5_4:2136187-2136206 None:intergenic 40.0%
GCACAACATTTACTACCTGA+AGG - chr5_4:2136190-2136209 None:intergenic 40.0%
GATTAAGGGTTTGGCTGATT+TGG + chr5_4:2136213-2136232 Msa0829210:CDS 40.0%
TTGTCTTTGCACTGCACTTA+AGG + chr5_4:2136246-2136265 Msa0829210:CDS 40.0%
!!! CGGTTTTTTGACATGCACTA+AGG - chr5_4:2136321-2136340 None:intergenic 40.0%
ATTGGAATCCTTAGGGACAT+TGG - chr5_4:2136357-2136376 None:intergenic 40.0%
TGTCCCTAAGGATTCCAATT+AGG + chr5_4:2136358-2136377 Msa0829210:intron 40.0%
!! TCTTTCTTTTAAGGGCGAAG+TGG + chr5_4:2137312-2137331 Msa0829210:intron 40.0%
ATTGTTGAAGCCAAAACACG+CGG - chr5_4:2137681-2137700 None:intergenic 40.0%
! CTCATAGATAAAATGGTGGG+TGG + chr5_4:2138633-2138652 Msa0829210:intron 40.0%
ACAAATGAGAAAAGGCCCAT+AGG + chr5_4:2138667-2138686 Msa0829210:intron 40.0%
TTGGATGATACAGAGCCTAT+GGG - chr5_4:2138685-2138704 None:intergenic 40.0%
TTTGGATGATACAGAGCCTA+TGG - chr5_4:2138686-2138705 None:intergenic 40.0%
!! AAGCTTACAAAAGTACCGAC+TGG + chr5_4:2138729-2138748 Msa0829210:intron 40.0%
!! CAATATGTGTGTTGGTTGAG+AGG - chr5_4:2138850-2138869 None:intergenic 40.0%
!! GAGAGAGAGTACTAAAATGG+TGG - chr5_4:2138929-2138948 None:intergenic 40.0%
CTCAACATTTACTGAGGCTA+AGG + chr5_4:2138957-2138976 Msa0829210:CDS 40.0%
AGCACAAACCCCTAACTTTA+AGG - chr5_4:2139055-2139074 None:intergenic 40.0%
!!! TTGTGCTGATGTTTTAGGAC+TGG + chr5_4:2139068-2139087 Msa0829210:CDS 40.0%
!! GGACTGGTTAATGTTGTTGT+TGG + chr5_4:2139084-2139103 Msa0829210:CDS 40.0%
CAACAATTACTACCTGAAGC+AGG - chr5_4:2139114-2139133 None:intergenic 40.0%
CAAGTTTATTCCGAGCACAT+TGG - chr5_4:2139199-2139218 None:intergenic 40.0%
TGTCAAAAAACAATCCCTCC+TGG + chr5_4:2139255-2139274 Msa0829210:CDS 40.0%
AGCTTCGGTGAATGTTGAGT+AGG - chr5_4:2136026-2136045 None:intergenic 45.0%
!! TCCTTAGGGCATTGACCTTT+GGG - chr5_4:2136103-2136122 None:intergenic 45.0%
!! ATCCTTAGGGCATTGACCTT+TGG - chr5_4:2136104-2136123 None:intergenic 45.0%
!! AGCACAAACCCCTAGCTTTA+AGG - chr5_4:2136128-2136147 None:intergenic 45.0%
!! GGGGTTTGTGCTGATCTTTT+AGG + chr5_4:2136136-2136155 Msa0829210:CDS 45.0%
GTTATTGGAAACCCTCCTTC+AGG + chr5_4:2136172-2136191 Msa0829210:CDS 45.0%
!! TAAGGGTTTGGCTGATTTGG+AGG + chr5_4:2136216-2136235 Msa0829210:CDS 45.0%
GCACTTAAGGCTAATGTGCT+TGG + chr5_4:2136259-2136278 Msa0829210:CDS 45.0%
TGCTGAACGCACGTAACATT+TGG - chr5_4:2136804-2136823 None:intergenic 45.0%
! CAAACACTAACCGCGTGTTT+TGG + chr5_4:2137668-2137687 Msa0829210:intron 45.0%
!! TTCGCTTAGCTTGCAAGTCA+CGG - chr5_4:2138134-2138153 None:intergenic 45.0%
TTCTCCACTTTCATGGCGTT+GGG - chr5_4:2138301-2138320 None:intergenic 45.0%
TTTCTCCACTTTCATGGCGT+TGG - chr5_4:2138302-2138321 None:intergenic 45.0%
!! GGTGGCAAAGAATTTGGTGT+TGG - chr5_4:2138911-2138930 None:intergenic 45.0%
!! TTTGGCTTTGGCTTTGGTGA+TGG - chr5_4:2138994-2139013 None:intergenic 45.0%
!! GGGGTTTGTGCTGATGTTTT+AGG + chr5_4:2139063-2139082 Msa0829210:CDS 45.0%
GCAATTAAGGCCAATGTGCT+CGG + chr5_4:2139186-2139205 Msa0829210:CDS 45.0%
AGGCACATTGAAAACCAGGA+GGG - chr5_4:2139272-2139291 None:intergenic 45.0%
TAGGCACATTGAAAACCAGG+AGG - chr5_4:2139273-2139292 None:intergenic 45.0%
!!! ATTTTTTTTTCTTATAATTT+AGG + chr5_4:2137116-2137135 Msa0829210:intron 5.0%
!! GAAGGTGTTGGCTTTGGTGA+TGG - chr5_4:2136058-2136077 None:intergenic 50.0%
CATTGACCTTTGGGTGAAGG+TGG - chr5_4:2136094-2136113 None:intergenic 50.0%
ACCCAAAGGTCAATGCCCTA+AGG + chr5_4:2136099-2136118 Msa0829210:CDS 50.0%
TGTCAAAAAACCGTCCCTCC+TGG + chr5_4:2136328-2136347 Msa0829210:CDS 50.0%
ACATTGGAAACCAGGAGGGA+CGG - chr5_4:2136341-2136360 None:intergenic 50.0%
AGGGACATTGGAAACCAGGA+GGG - chr5_4:2136345-2136364 None:intergenic 50.0%
TAGGGACATTGGAAACCAGG+AGG - chr5_4:2136346-2136365 None:intergenic 50.0%
CCTTAGGGACATTGGAAACC+AGG - chr5_4:2136349-2136368 None:intergenic 50.0%
CCTGGTTTCCAATGTCCCTA+AGG + chr5_4:2136346-2136365 Msa0829210:intron 50.0%
TTAAGGGCGAAGTGGTAGCA+CGG + chr5_4:2137320-2137339 Msa0829210:intron 50.0%
!! TCGCTTAGCTTGCAAGTCAC+GGG - chr5_4:2138133-2138152 None:intergenic 50.0%
CAAACCCAACGCCATGAAAG+TGG + chr5_4:2138294-2138313 Msa0829210:intron 50.0%
!! GTTGGCTTTGGCTTTGGCTT+TGG - chr5_4:2139000-2139019 None:intergenic 50.0%
!! GAAGGTGTTGGCTTTGGCTT+TGG - chr5_4:2139006-2139025 None:intergenic 50.0%
!! ATCTTTGGGGCAGTGACCTT+GGG - chr5_4:2139031-2139050 None:intergenic 50.0%
!! TATCTTTGGGGCAGTGACCT+TGG - chr5_4:2139032-2139051 None:intergenic 50.0%
GTTGTTGGGAATCCTGCTTC+AGG + chr5_4:2139099-2139118 Msa0829210:CDS 50.0%
ATGTACCTATCACCCTCACC+TGG + chr5_4:2139220-2139239 Msa0829210:CDS 50.0%
TTGACAAGCACCAAGGATCC+AGG - chr5_4:2139241-2139260 None:intergenic 50.0%
!! GGTGTTGGCTTTGGTGATGG+TGG - chr5_4:2136055-2136074 None:intergenic 55.0%
!! GCAGGTGAAGGTGTTGGCTT+TGG - chr5_4:2136064-2136083 None:intergenic 55.0%
! ACAGGAGCAGGTGAAGGTGT+TGG - chr5_4:2136070-2136089 None:intergenic 55.0%
CCTTTGGGTGAAGGTGGAAC+AGG - chr5_4:2136088-2136107 None:intergenic 55.0%
CCTGTTCCACCTTCACCCAA+AGG + chr5_4:2136085-2136104 Msa0829210:CDS 55.0%
!! GGGCATTGACCTTTGGGTGA+AGG - chr5_4:2136097-2136116 None:intergenic 55.0%
!! TGGGGTGAAGGTGTTGGCTT+TGG - chr5_4:2139012-2139031 None:intergenic 55.0%
AAGCCAACACCTTCACCCCA+AGG + chr5_4:2139012-2139031 Msa0829210:CDS 55.0%
! TGACCTTGGGGTGAAGGTGT+TGG - chr5_4:2139018-2139037 None:intergenic 55.0%
!! TCTTTGGGGCAGTGACCTTG+GGG - chr5_4:2139030-2139049 None:intergenic 55.0%
AGGATCCAGGTGAGGGTGAT+AGG - chr5_4:2139228-2139247 None:intergenic 55.0%
ATCACCCTCACCTGGATCCT+TGG + chr5_4:2139228-2139247 Msa0829210:CDS 55.0%
AGCACCAAGGATCCAGGTGA+GGG - chr5_4:2139235-2139254 None:intergenic 55.0%
AAGCACCAAGGATCCAGGTG+AGG - chr5_4:2139236-2139255 None:intergenic 55.0%
GGTGGAACAGGAGCAGGTGA+AGG - chr5_4:2136076-2136095 None:intergenic 60.0%
GGTGAAGGTGGAACAGGAGC+AGG - chr5_4:2136082-2136101 None:intergenic 60.0%
! AGGAGCACGCTGAGCGTGAT+AGG - chr5_4:2136301-2136320 None:intergenic 60.0%
GGGCAGTGACCTTGGGGTGA+AGG - chr5_4:2139024-2139043 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 2135974 2139293 2135974 ID=Msa0829210;Name=Msa0829210
chr5_4 mRNA 2135974 2139293 2135974 ID=Msa0829210-mRNA-1;Parent=Msa0829210;Name=Msa0829210-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|2|0|256
chr5_4 exon 2135974 2136349 2135974 ID=Msa0829210-mRNA-1:exon:645;Parent=Msa0829210-mRNA-1
chr5_4 exon 2138899 2139293 2138899 ID=Msa0829210-mRNA-1:exon:646;Parent=Msa0829210-mRNA-1
chr5_4 CDS 2135974 2136349 2135974 ID=Msa0829210-mRNA-1:cds;Parent=Msa0829210-mRNA-1
chr5_4 CDS 2138899 2139293 2138899 ID=Msa0829210-mRNA-1:cds;Parent=Msa0829210-mRNA-1
Gene Sequence

>Msa0829210

ATGGCTTCCAACAAATTCTCTGCCTTAATTTTAGTGCTTTCTCTTCTTGCCTACTCAACATTCACCGAAGCTAATTGTCCACCATCACCAAAGCCAACACCTTCACCTGCTCCTGTTCCACCTTCACCCAAAGGTCAATGCCCTAAGGATACCTTAAAGCTAGGGGTTTGTGCTGATCTTTTAGGGTTGGTTAATGTTGTTATTGGAAACCCTCCTTCAGGTAGTAAATGTTGTGCATTGATTAAGGGTTTGGCTGATTTGGAGGCTGCACTTTGTCTTTGCACTGCACTTAAGGCTAATGTGCTTGGAATCAACTTGAACGTGCCTATCACGCTCAGCGTGCTCCTTAGTGCATGTCAAAAAACCGTCCCTCCTGTCATGGCTTCCAACACCAAATTCTTTGCCACCATTTTAGTACTCTCTCTCCTTGCTTACTCAACATTTACTGAGGCTAAGGGTTCATGCTCACCATCACCAAAGCCAAAGCCAAAGCCAACACCTTCACCCCAAGGTCACTGCCCCAAAGATACCTTAAAGTTAGGGGTTTGTGCTGATGTTTTAGGACTGGTTAATGTTGTTGTTGGGAATCCTGCTTCAGGTAGTAATTGTTGTGCAATAATCAAAGGTTTGGCTGATTTGGATGCTGCACTTTGTCTTTGCACTGCAATTAAGGCCAATGTGCTCGGAATAAACTTGAATGTACCTATCACCCTCACCTGGATCCTTGGTGCTTGTCAAAAAACAATCCCTCCTGGTTTTCAATGTGCCTAA
Protein sequence

>Msa0829210

MASNKFSALILVLSLLAYSTFTEANCPPSPKPTPSPAPVPPSPKGQCPKDTLKLGVCADLLGLVNVVIGNPPSGSKCCALIKGLADLEAALCLCTALKANVLGINLNVPITLSVLLSACQKTVPPVMASNTKFFATILVLSLLAYSTFTEAKGSCSPSPKPKPKPTPSPQGHCPKDTLKLGVCADVLGLVNVVVGNPASGSNCCAIIKGLADLDAALCLCTAIKANVLGINLNVPITLTWILGACQKTIPPGFQCA*