AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0457600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0457600 MtrunA17_Chr3g0092091 99.209 253 2 0 1 253 1 253 0.0 517
Msa0457600 MtrunA17_Chr8g0345631 50.000 244 112 5 15 250 25 266 3.22e-75 229
Msa0457600 MtrunA17_Chr2g0301081 51.724 203 92 2 56 252 65 267 4.58e-68 211
Msa0457600 MtrunA17_Chr3g0133901 50.725 207 94 4 52 250 57 263 3.16e-65 204
Msa0457600 MtrunA17_Chr2g0296081 93.103 87 6 0 167 253 19 105 1.65e-54 171
Msa0457600 MtrunA17_Chr5g0448411 42.279 272 111 11 18 249 7 272 2.51e-48 160
Msa0457600 MtrunA17_Chr5g0448421 40.727 275 127 10 2 249 9 274 8.44e-48 159
Msa0457600 MtrunA17_Chr6g0462031 39.381 226 106 7 38 249 30 238 6.16e-43 145
Msa0457600 MtrunA17_Chr6g0476641 38.614 202 100 5 63 251 91 281 8.88e-33 120
Msa0457600 MtrunA17_Chr1g0157481 37.736 212 100 8 63 250 125 328 1.80e-32 121
Msa0457600 MtrunA17_Chr2g0279001 37.619 210 95 7 53 233 57 259 6.02e-31 115
Msa0457600 MtrunA17_Chr6g0453141 36.041 197 102 5 62 243 65 252 1.97e-30 114
Msa0457600 MtrunA17_Chr6g0453151 36.041 197 102 5 62 243 132 319 3.74e-30 115
Msa0457600 MtrunA17_Chr6g0453181 35.533 197 103 5 62 243 65 252 5.24e-30 113
Msa0457600 MtrunA17_Chr4g0051941 35.714 196 102 5 63 243 67 253 6.83e-30 112
Msa0457600 MtrunA17_Chr4g0006181 33.071 254 88 6 56 250 115 345 1.03e-29 114
Msa0457600 MtrunA17_Chr6g0453161 36.041 197 102 5 62 243 67 254 1.19e-28 113
Msa0457600 MtrunA17_Chr6g0453161 35.533 197 103 5 62 243 332 519 1.45e-28 113
Msa0457600 MtrunA17_Chr5g0447531 34.826 201 97 6 63 243 66 252 1.05e-27 107
Msa0457600 MtrunA17_Chr2g0318521 37.500 200 99 6 62 244 62 252 5.61e-27 105
Msa0457600 MtrunA17_Chr3g0113311 33.476 233 73 8 56 229 60 269 3.71e-23 95.1
Msa0457600 MtrunA17_Chr6g0464511 33.871 186 87 6 62 243 46 199 9.63e-19 82.0
Msa0457600 MtrunA17_Chr6g0480171 86.486 37 5 0 217 253 29 65 1.60e-17 74.7
Msa0457600 MtrunA17_Chr6g0453171 48.649 74 31 2 178 244 13 86 5.80e-13 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0457600 AT3G47470.1 88.446 251 28 1 1 250 1 251 3.00e-166 459
Msa0457600 AT3G61470.1 52.703 222 96 3 38 250 34 255 1.63e-74 227
Msa0457600 AT1G45474.2 51.905 210 92 4 49 249 42 251 3.22e-68 211
Msa0457600 AT1G45474.1 51.905 210 92 4 49 249 42 251 3.22e-68 211
Msa0457600 AT1G19150.1 44.681 235 122 2 24 250 34 268 1.17e-66 208
Msa0457600 AT1G61520.3 44.978 229 93 9 48 249 48 270 2.74e-46 155
Msa0457600 AT1G61520.1 44.978 229 93 9 48 249 48 270 2.74e-46 155
Msa0457600 AT1G61520.2 46.154 208 79 9 69 249 14 215 2.50e-43 146
Msa0457600 AT3G54890.1 41.176 204 90 6 55 245 47 233 4.42e-42 144
Msa0457600 AT3G08940.2 31.359 287 115 8 23 250 22 285 4.67e-31 116
Msa0457600 AT2G05070.1 35.644 202 96 6 62 243 65 252 1.26e-30 115
Msa0457600 AT3G54890.2 35.000 200 74 4 55 245 47 199 1.27e-30 113
Msa0457600 AT2G05100.1 35.644 202 96 6 62 243 65 252 1.43e-30 114
Msa0457600 AT1G29930.1 35.567 194 108 4 62 243 66 254 2.03e-30 114
Msa0457600 AT1G29920.1 35.567 194 108 4 62 243 66 254 2.09e-30 114
Msa0457600 AT1G29910.1 35.567 194 108 4 62 243 66 254 2.09e-30 114
Msa0457600 AT2G34420.1 32.046 259 153 7 1 243 1 252 2.23e-30 114
Msa0457600 AT2G34430.1 32.900 231 134 6 27 243 30 253 2.32e-30 114
Msa0457600 AT1G76570.1 38.208 212 99 8 63 250 123 326 3.88e-30 115
Msa0457600 AT5G01530.1 32.567 261 94 6 49 250 51 288 7.53e-30 113
Msa0457600 AT3G27690.1 35.149 202 97 6 62 243 66 253 9.73e-30 112
Msa0457600 AT3G27690.2 35.149 202 97 6 62 243 98 285 1.71e-29 112
Msa0457600 AT4G10340.1 37.500 200 105 6 63 251 82 272 4.60e-29 111
Msa0457600 AT5G54270.1 38.308 201 98 6 60 243 61 252 8.97e-28 107
Msa0457600 AT1G15820.1 33.594 256 122 12 16 233 11 256 2.19e-27 106
Msa0457600 AT3G54890.4 37.143 175 80 6 55 216 47 204 7.19e-25 98.6
Msa0457600 AT5G28450.1 50.495 101 44 3 156 250 71 171 1.47e-22 91.3
Msa0457600 AT2G05100.2 33.862 189 91 6 62 230 65 239 4.20e-22 93.6
Msa0457600 AT2G40100.1 31.466 232 78 6 56 229 59 267 3.65e-21 90.1
Msa0457600 AT3G54890.3 38.739 111 59 3 55 156 47 157 1.69e-17 77.8

Find 127 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATTACAATTCTTTGAATT+TGG 0.102937 3_3:+52624838 Msa0457600:three_prime_UTR
CTTTCAAAATTCATCCATTC+AGG 0.215050 3_3:-52624711 None:intergenic
TTCCCTGCATCGTACCATTT+TGG 0.221678 3_3:-52623942 None:intergenic
TTTATTTGAGAGTGATCTTA+TGG 0.239666 3_3:-52625400 None:intergenic
TGGTAATTTAATGTAGAAAA+TGG 0.245998 3_3:-52625152 None:intergenic
ATTGGCAATGTTAGCATTCT+TGG 0.263254 3_3:+52624525 Msa0457600:CDS
CAAACCACATGGCCGGAAAA+TGG 0.281257 3_3:-52622817 None:intergenic
CCAATGTTGGTGAAGACTTC+AGG 0.283187 3_3:-52623906 None:intergenic
GTTCCTCTGTACATAGAAAT+AGG 0.308373 3_3:-52624758 None:intergenic
AGGTTCCTTACTGGTTCTTC+TGG 0.312510 3_3:+52622846 Msa0457600:CDS
TTGAAATCAAGGTTCCTTAC+TGG 0.312689 3_3:+52622837 Msa0457600:CDS
AACATAGGCTTTCATGTCAT+TGG 0.323632 3_3:-52624675 None:intergenic
GATCTTATGGAAACAACTTA+TGG 0.352510 3_3:-52625387 None:intergenic
GAATCATCAACGTTCCAAAA+TGG 0.354053 3_3:+52623928 Msa0457600:CDS
TGTATTGATATTAGTCTTCC+CGG 0.354614 3_3:+52623765 Msa0457600:intron
TGAACAGAGAAGTGGCTATT+AGG 0.355262 3_3:+52622874 Msa0457600:CDS
GGCCATGTGGTTTGAAATCA+AGG 0.359010 3_3:+52622826 Msa0457600:CDS
TGGGCTATGTTAGGTGTTGC+AGG 0.363791 3_3:+52623873 Msa0457600:CDS
AGGAGAGTGGTTGCCTGGTT+TGG 0.364447 3_3:+52622944 Msa0457600:CDS
GAGATATAGTAATGAGATAT+AGG 0.366128 3_3:-52625098 None:intergenic
TGAAACAGTTCTCTATCGTT+AGG 0.379493 3_3:+52624883 Msa0457600:three_prime_UTR
ACAAACCACTTCAAGTTCTC+TGG 0.381297 3_3:-52623825 None:intergenic
TCAAATGTTTGCAGGGAGAT+TGG 0.385168 3_3:+52624507 Msa0457600:intron
TTCTCTTTAGCTTCCAGAGT+TGG 0.397152 3_3:-52624158 None:intergenic
ATTTGAACCGTGACTTTCAA+TGG 0.407460 3_3:+52624987 Msa0457600:three_prime_UTR
TTGGCAATGTTAGCATTCTT+GGG 0.411192 3_3:+52624526 Msa0457600:CDS
CGATAGAGAACTGTTTCAAA+AGG 0.419396 3_3:-52624878 None:intergenic
GCTTCCCCTGGTTACCTCAC+TGG 0.420500 3_3:+52622966 Msa0457600:CDS
TTCCTTATTTCTAAACCCTC+TGG 0.427167 3_3:-52625460 None:intergenic
ATTGCTTGAAGATAGGATCT+TGG 0.434539 3_3:-52624073 None:intergenic
TTGCCTGGTTTGGCTTCCCC+TGG 0.440427 3_3:+52622954 Msa0457600:CDS
TTTGAACTTTGCACCAACTC+TGG 0.443047 3_3:+52624145 Msa0457600:CDS
TTTAAGGTTGAAGCTAAGAA+AGG 0.444868 3_3:+52622924 Msa0457600:CDS
GTCATTCAGCACAATGTGAC+AGG 0.452299 3_3:+52624553 Msa0457600:CDS
CAGCACAATGTGACAGGAAA+AGG 0.457752 3_3:+52624559 Msa0457600:CDS
CGGTTCAAATGCTCAGTCCT+AGG 0.458778 3_3:-52624974 None:intergenic
TAACCAGGGGAAGCCAAACC+AGG 0.461430 3_3:-52622957 None:intergenic
AAATTACCAACTTGGTTCAC+AGG 0.463934 3_3:+52625166 Msa0457600:three_prime_UTR
GAAACAGTTCTCTATCGTTA+GGG 0.464877 3_3:+52624884 Msa0457600:three_prime_UTR
CAATGTTGGTGAAGACTTCA+GGG 0.467740 3_3:-52623905 None:intergenic
ATCTTCTAATCTCGACGTAA+TGG 0.468668 3_3:-52624019 None:intergenic
GAACAATGGTGTTGTGCCAT+GGG 0.471293 3_3:-52624615 None:intergenic
AAGCTGTATTGCTTGAAGAT+AGG 0.474202 3_3:-52624080 None:intergenic
GTTCAAGCCGAGCTTGTGAA+TGG 0.475321 3_3:+52623846 Msa0457600:CDS
TACCTGCCAGTGAGGTAACC+AGG 0.476834 3_3:-52622972 None:intergenic
TGAACAATGGTGTTGTGCCA+TGG 0.478119 3_3:-52624616 None:intergenic
AAGAAAGGAGAGTGGTTGCC+TGG 0.478204 3_3:+52622939 Msa0457600:CDS
GGGTAACCAACCTCACCCTT+TGG 0.479854 3_3:-52624104 None:intergenic
GTTCCAAAATGGTACGATGC+AGG 0.482951 3_3:+52623939 Msa0457600:CDS
CCTGAAGTCTTCACCAACAT+TGG 0.483026 3_3:+52623906 Msa0457600:CDS
AATGGACGTTGGGCTATGTT+AGG 0.483699 3_3:+52623864 Msa0457600:CDS
AGACAATGGATTCGATCCAT+TGG 0.488323 3_3:+52623788 Msa0457600:CDS
AGAGTTGGTGCAAAGTTCAA+AGG 0.492262 3_3:-52624143 None:intergenic
CGAGCTTGTGAATGGACGTT+GGG 0.494207 3_3:+52623854 Msa0457600:CDS
GATAGAGAACTGTTTCAAAA+GGG 0.495143 3_3:-52624877 None:intergenic
GGATTACATTAGAATAACAT+AGG 0.495974 3_3:-52624690 None:intergenic
GACAATGGATTCGATCCATT+GGG 0.497505 3_3:+52623789 Msa0457600:CDS
TAATTTCAAGCAGACCTGCC+AGG 0.499619 3_3:+52625423 Msa0457600:three_prime_UTR
AAAGAGAAGGAAATTGCTAA+TGG 0.501630 3_3:+52624173 Msa0457600:CDS
AGCTTGCCAAAGGGTGAGGT+TGG 0.502406 3_3:+52624098 Msa0457600:CDS
AGTGGCCATGTTCAACTACT+AGG 0.503254 3_3:-52622772 None:intergenic
AAGTCACATTGGACCCTGGC+AGG 0.512175 3_3:-52625437 None:intergenic
TGGATCGAATCCATTGTCTC+CGG 0.512990 3_3:-52623784 None:intergenic
TTTGATCCTGTGAACCAAGT+TGG 0.514498 3_3:-52625172 None:intergenic
TCTACATTAAATTACCAACT+TGG 0.520354 3_3:+52625158 Msa0457600:three_prime_UTR
GAAGTGGCTATTAGGCCAAT+GGG 0.521329 3_3:+52622882 Msa0457600:CDS
CCCCTGGTTACCTCACTGGC+AGG 0.522153 3_3:+52622970 Msa0457600:CDS
GGATCTTGGTTAACACTACC+AGG 0.527012 3_3:-52624059 None:intergenic
AACGTTGATGATTCCAATGT+TGG 0.527016 3_3:-52623919 None:intergenic
TGCAGCACCTTTCAGACCCA+TGG 0.527064 3_3:+52624599 Msa0457600:CDS
ACCATTGTTCAAACACTAAG+TGG 0.529535 3_3:+52624628 Msa0457600:CDS
GAGTTGGTGCAAAGTTCAAA+GGG 0.532634 3_3:-52624142 None:intergenic
TATTCTAATGTAATCCTGAA+TGG 0.532739 3_3:+52624697 Msa0457600:three_prime_UTR
AAGGGTGAGGTTGGTTACCC+CGG 0.533098 3_3:+52624107 Msa0457600:CDS
TTGATTTCAAACCACATGGC+CGG 0.533466 3_3:-52622824 None:intergenic
GGATCCTCGGCTAGTCCCAA+TGG 0.534357 3_3:-52623804 None:intergenic
CTTCAAGTTCTCTGGATCCT+CGG 0.540375 3_3:-52623817 None:intergenic
AGAAGTGGCTATTAGGCCAA+TGG 0.543373 3_3:+52622881 Msa0457600:CDS
ATTAGTCTTCCCGGAGACAA+TGG 0.543395 3_3:+52623774 Msa0457600:intron
GGATCGAATCCATTGTCTCC+GGG 0.543577 3_3:-52623783 None:intergenic
TTCCAAAATGGTACGATGCA+GGG 0.551197 3_3:+52623940 Msa0457600:CDS
TGCTGCAAGAGGTTATCAAA+TGG 0.555368 3_3:-52624583 None:intergenic
ATACAGCTTGCCAAAGGGTG+AGG 0.558596 3_3:+52624094 Msa0457600:CDS
CTATGTACAGAGGAACTAAG+TGG 0.559926 3_3:+52624765 Msa0457600:three_prime_UTR
AAACCCTCTGGAAGTCACAT+TGG 0.563919 3_3:-52625448 None:intergenic
AAAATGGCTTTCTAAGTGGC+GGG 0.565209 3_3:-52624910 None:intergenic
CCATTGTTCAAACACTAAGT+GGG 0.567400 3_3:+52624629 Msa0457600:CDS
CAAGCAATACAGCTTGCCAA+AGG 0.568836 3_3:+52624088 Msa0457600:CDS
ATTACGTCGAGATTAGAAGA+TGG 0.573199 3_3:+52624021 Msa0457600:CDS
AAGCAATACAGCTTGCCAAA+GGG 0.573830 3_3:+52624089 Msa0457600:CDS
AATTTCAAGCAGACCTGCCA+GGG 0.581082 3_3:+52625424 Msa0457600:three_prime_UTR
CTCGAGACTTCCTCATGCCT+AGG 0.582653 3_3:+52624957 Msa0457600:three_prime_UTR
ACTAGGAATAATAGTAGTAG+TGG 0.582693 3_3:-52622755 None:intergenic
CTGGAAGTCACATTGGACCC+TGG 0.585836 3_3:-52625441 None:intergenic
ACTTACCAGAAGAACCAGTA+AGG 0.587465 3_3:-52622851 None:intergenic
TGTTGTGCCATGGGTCTGAA+AGG 0.587745 3_3:-52624606 None:intergenic
CCGAGCTTGTGAATGGACGT+TGG 0.595865 3_3:+52623853 Msa0457600:CDS
AACTCTGGAAGCTAAAGAGA+AGG 0.599977 3_3:+52624160 Msa0457600:CDS
CGATCCATTGGGACTAGCCG+AGG 0.601654 3_3:+52623800 Msa0457600:CDS
ACCTGCCAGTGAGGTAACCA+GGG 0.604553 3_3:-52622971 None:intergenic
CCAACGTCCATTCACAAGCT+CGG 0.607811 3_3:-52623853 None:intergenic
TGGTAAGTTGAACAGAGAAG+TGG 0.611762 3_3:+52622866 Msa0457600:CDS
GGGTCCAATGTGACTTCCAG+AGG 0.612084 3_3:+52625444 Msa0457600:three_prime_UTR
GGGCTATGTTAGGTGTTGCA+GGG 0.612177 3_3:+52623874 Msa0457600:CDS
GCAGAAGGTGAACAACCCAT+TGG 0.616203 3_3:-52622897 None:intergenic
CCCACTTAGTGTTTGAACAA+TGG 0.617047 3_3:-52624629 None:intergenic
AACCTTGATTTCAAACCACA+TGG 0.619985 3_3:-52622828 None:intergenic
CATTGTTCAAACACTAAGTG+GGG 0.620145 3_3:+52624630 Msa0457600:CDS
TTGAAGCTAAGAAAGGAGAG+TGG 0.624150 3_3:+52622931 Msa0457600:CDS
AGGATCCAGAGAACTTGAAG+TGG 0.633648 3_3:+52623820 Msa0457600:CDS
TGGCAAGACATTAAGAACCC+TGG 0.635088 3_3:+52624041 Msa0457600:CDS
CATGGCCACTGTTACTACAC+AGG 0.640157 3_3:+52622785 Msa0457600:exon
GATCCTATTTCTATGTACAG+AGG 0.648589 3_3:+52624755 Msa0457600:three_prime_UTR
GGTCTGAAAGGTGCTGCAAG+AGG 0.650437 3_3:-52624594 None:intergenic
GATTGACATACCTGCCAGTG+AGG 0.658865 3_3:-52622980 None:intergenic
TTATGAAAACAAATTGACAA+CGG 0.660029 3_3:+52625353 Msa0457600:three_prime_UTR
GGTCCAATGTGACTTCCAGA+GGG 0.660490 3_3:+52625445 Msa0457600:three_prime_UTR
TCAACCTTAAAAGAAGCAGA+AGG 0.663014 3_3:-52622912 None:intergenic
TTATTCCTAGTAGTTGAACA+TGG 0.665521 3_3:+52622767 Msa0457600:exon
AATGCTCAGTCCTAGGCATG+AGG 0.667162 3_3:-52624967 None:intergenic
GGTGAGGTTGGTTACCCCGG+TGG 0.669754 3_3:+52624110 Msa0457600:CDS
GATCTTGGTTAACACTACCA+GGG 0.677002 3_3:-52624058 None:intergenic
AGAAGCCTGTGTAGTAACAG+TGG 0.678873 3_3:-52622790 None:intergenic
ATCGTGGCCATTGAAAGTCA+CGG 0.691350 3_3:-52624994 None:intergenic
AGAGTCTACTGAGATAATCG+TGG 0.707102 3_3:-52625010 None:intergenic
ATTGTTCAAACACTAAGTGG+GGG 0.707392 3_3:+52624631 Msa0457600:CDS
CCTGCCAGTGAGGTAACCAG+GGG 0.743123 3_3:-52622970 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATTAGTATACTATTTTG+TGG + chr3_3:52623009-52623028 Msa0457600:intron 15.0%
!!! AGCTATTATTTTATTAGTAT+AGG + chr3_3:52623254-52623273 Msa0457600:intron 15.0%
!!! AATTTTAATCCTTATGAAAT+CGG - chr3_3:52624436-52624455 None:intergenic 15.0%
!!! CTAAAACTATTTTAGAATTT+AGG + chr3_3:52625042-52625061 Msa0457600:three_prime_UTR 15.0%
!!! AATGAAGATGAAAATGTTTT+AGG - chr3_3:52622706-52622725 None:intergenic 20.0%
!! AGTGACAAAGAAAAAAATAA+AGG - chr3_3:52622734-52622753 None:intergenic 20.0%
!! ATTAGTTATAGTTTGAAAAG+TGG + chr3_3:52623082-52623101 Msa0457600:intron 20.0%
!! TTGATTATGTTAATTAGATG+TGG - chr3_3:52624407-52624426 None:intergenic 20.0%
!! TAGTTTCATAATCAAAAAAC+AGG - chr3_3:52624467-52624486 None:intergenic 20.0%
!!! GTTTTTTGATTATGAAACTA+TGG + chr3_3:52624467-52624486 Msa0457600:intron 20.0%
!! CTATTACAATTCTTTGAATT+TGG + chr3_3:52624838-52624857 Msa0457600:three_prime_UTR 20.0%
!!! AACTATTTTAGAATTTAGGT+TGG + chr3_3:52625046-52625065 Msa0457600:three_prime_UTR 20.0%
!! TGGTAATTTAATGTAGAAAA+TGG - chr3_3:52625155-52625174 None:intergenic 20.0%
!!! TGTTTTCATAAACTCTTTTT+AGG - chr3_3:52625344-52625363 None:intergenic 20.0%
!! TTATGAAAACAAATTGACAA+CGG + chr3_3:52625353-52625372 Msa0457600:three_prime_UTR 20.0%
! TTGAATATTGATCTGTATGT+TGG - chr3_3:52623208-52623227 None:intergenic 25.0%
! ATCAAAGTCTTTGCTTAAAT+GGG + chr3_3:52624235-52624254 Msa0457600:intron 25.0%
! CTAAATTATCCCATCTAAAT+TGG - chr3_3:52624342-52624361 None:intergenic 25.0%
! GGATTACATTAGAATAACAT+AGG - chr3_3:52624693-52624712 None:intergenic 25.0%
! TATTCTAATGTAATCCTGAA+TGG + chr3_3:52624697-52624716 Msa0457600:three_prime_UTR 25.0%
!!! ATGAATTTTGAAAGACATCT+GGG + chr3_3:52624720-52624739 Msa0457600:three_prime_UTR 25.0%
! GAGATATAGTAATGAGATAT+AGG - chr3_3:52625101-52625120 None:intergenic 25.0%
! TCTACATTAAATTACCAACT+TGG + chr3_3:52625158-52625177 Msa0457600:three_prime_UTR 25.0%
! TTTATTTGAGAGTGATCTTA+TGG - chr3_3:52625403-52625422 None:intergenic 25.0%
ACTAGGAATAATAGTAGTAG+TGG - chr3_3:52622758-52622777 None:intergenic 30.0%
TTATTCCTAGTAGTTGAACA+TGG + chr3_3:52622767-52622786 Msa0457600:exon 30.0%
! TTTAAGGTTGAAGCTAAGAA+AGG + chr3_3:52622924-52622943 Msa0457600:CDS 30.0%
AGTATGACACTAAAACATGT+CGG + chr3_3:52623153-52623172 Msa0457600:intron 30.0%
TATGCATAAGCTCAACTTAA+GGG - chr3_3:52623235-52623254 None:intergenic 30.0%
TTTAAGTGTACACTTGATGA+AGG + chr3_3:52623668-52623687 Msa0457600:intron 30.0%
!! TGTATTGATATTAGTCTTCC+CGG + chr3_3:52623765-52623784 Msa0457600:intron 30.0%
AAAGAGAAGGAAATTGCTAA+TGG + chr3_3:52624173-52624192 Msa0457600:CDS 30.0%
CATCAAAGTCTTTGCTTAAA+TGG + chr3_3:52624234-52624253 Msa0457600:intron 30.0%
! CAAATTTGACCGATTTCATA+AGG + chr3_3:52624424-52624443 Msa0457600:intron 30.0%
TGTGTATTTCAAATGTTTGC+AGG + chr3_3:52624499-52624518 Msa0457600:intron 30.0%
GTGTATTTCAAATGTTTGCA+GGG + chr3_3:52624500-52624519 Msa0457600:intron 30.0%
CTTTCAAAATTCATCCATTC+AGG - chr3_3:52624714-52624733 None:intergenic 30.0%
!! GATGAATTTTGAAAGACATC+TGG + chr3_3:52624719-52624738 Msa0457600:three_prime_UTR 30.0%
GATAGAGAACTGTTTCAAAA+GGG - chr3_3:52624880-52624899 None:intergenic 30.0%
GATGAGATATGTTCCAAAAA+TGG - chr3_3:52624929-52624948 None:intergenic 30.0%
GATCTTATGGAAACAACTTA+TGG - chr3_3:52625390-52625409 None:intergenic 30.0%
AACCTTGATTTCAAACCACA+TGG - chr3_3:52622831-52622850 None:intergenic 35.0%
TTGAAATCAAGGTTCCTTAC+TGG + chr3_3:52622837-52622856 Msa0457600:CDS 35.0%
! TTCACCTTCTGCTTCTTTTA+AGG + chr3_3:52622908-52622927 Msa0457600:CDS 35.0%
TCAACCTTAAAAGAAGCAGA+AGG - chr3_3:52622915-52622934 None:intergenic 35.0%
ATGCATAAGCTCAACTTAAG+GGG - chr3_3:52623234-52623253 None:intergenic 35.0%
CTATGCATAAGCTCAACTTA+AGG - chr3_3:52623236-52623255 None:intergenic 35.0%
!! AACGTTGATGATTCCAATGT+TGG - chr3_3:52623922-52623941 None:intergenic 35.0%
GAATCATCAACGTTCCAAAA+TGG + chr3_3:52623928-52623947 Msa0457600:CDS 35.0%
ATCTTCTAATCTCGACGTAA+TGG - chr3_3:52624022-52624041 None:intergenic 35.0%
ATTACGTCGAGATTAGAAGA+TGG + chr3_3:52624021-52624040 Msa0457600:CDS 35.0%
ATTGCTTGAAGATAGGATCT+TGG - chr3_3:52624076-52624095 None:intergenic 35.0%
AAGCTGTATTGCTTGAAGAT+AGG - chr3_3:52624083-52624102 None:intergenic 35.0%
AAAGGGTTAAAAATTCCACC+GGG - chr3_3:52624128-52624147 None:intergenic 35.0%
CAAAGGGTTAAAAATTCCAC+CGG - chr3_3:52624129-52624148 None:intergenic 35.0%
AGACTTTGATGCAGTATTTC+AGG - chr3_3:52624225-52624244 None:intergenic 35.0%
ATCCCATCTAAATTGGTACT+GGG - chr3_3:52624335-52624354 None:intergenic 35.0%
TATCCCATCTAAATTGGTAC+TGG - chr3_3:52624336-52624355 None:intergenic 35.0%
ATTGGCAATGTTAGCATTCT+TGG + chr3_3:52624525-52624544 Msa0457600:CDS 35.0%
TTGGCAATGTTAGCATTCTT+GGG + chr3_3:52624526-52624545 Msa0457600:CDS 35.0%
ACCATTGTTCAAACACTAAG+TGG + chr3_3:52624628-52624647 Msa0457600:CDS 35.0%
CCATTGTTCAAACACTAAGT+GGG + chr3_3:52624629-52624648 Msa0457600:CDS 35.0%
CATTGTTCAAACACTAAGTG+GGG + chr3_3:52624630-52624649 Msa0457600:CDS 35.0%
ATTGTTCAAACACTAAGTGG+GGG + chr3_3:52624631-52624650 Msa0457600:CDS 35.0%
AACATAGGCTTTCATGTCAT+TGG - chr3_3:52624678-52624697 None:intergenic 35.0%
!! GATCCTATTTCTATGTACAG+AGG + chr3_3:52624755-52624774 Msa0457600:three_prime_UTR 35.0%
GTTCCTCTGTACATAGAAAT+AGG - chr3_3:52624761-52624780 None:intergenic 35.0%
CGATAGAGAACTGTTTCAAA+AGG - chr3_3:52624881-52624900 None:intergenic 35.0%
TGAAACAGTTCTCTATCGTT+AGG + chr3_3:52624883-52624902 Msa0457600:three_prime_UTR 35.0%
GAAACAGTTCTCTATCGTTA+GGG + chr3_3:52624884-52624903 Msa0457600:three_prime_UTR 35.0%
AAAAATGGCTTTCTAAGTGG+CGG - chr3_3:52624914-52624933 None:intergenic 35.0%
TCCAAAAATGGCTTTCTAAG+TGG - chr3_3:52624917-52624936 None:intergenic 35.0%
ATTTGAACCGTGACTTTCAA+TGG + chr3_3:52624987-52625006 Msa0457600:three_prime_UTR 35.0%
AAATTACCAACTTGGTTCAC+AGG + chr3_3:52625166-52625185 Msa0457600:three_prime_UTR 35.0%
TTGATTTCAAACCACATGGC+CGG - chr3_3:52622827-52622846 None:intergenic 40.0%
ACTTACCAGAAGAACCAGTA+AGG - chr3_3:52622854-52622873 None:intergenic 40.0%
! TGGTAAGTTGAACAGAGAAG+TGG + chr3_3:52622866-52622885 Msa0457600:CDS 40.0%
TGAACAGAGAAGTGGCTATT+AGG + chr3_3:52622874-52622893 Msa0457600:CDS 40.0%
TTGAAGCTAAGAAAGGAGAG+TGG + chr3_3:52622931-52622950 Msa0457600:CDS 40.0%
TGCATAAGCTCAACTTAAGG+GGG - chr3_3:52623233-52623252 None:intergenic 40.0%
! AGACAATGGATTCGATCCAT+TGG + chr3_3:52623788-52623807 Msa0457600:CDS 40.0%
! GACAATGGATTCGATCCATT+GGG + chr3_3:52623789-52623808 Msa0457600:CDS 40.0%
! ACAAACCACTTCAAGTTCTC+TGG - chr3_3:52623828-52623847 None:intergenic 40.0%
! CAATGTTGGTGAAGACTTCA+GGG - chr3_3:52623908-52623927 None:intergenic 40.0%
TTCCAAAATGGTACGATGCA+GGG + chr3_3:52623940-52623959 Msa0457600:CDS 40.0%
GATCTTGGTTAACACTACCA+GGG - chr3_3:52624061-52624080 None:intergenic 40.0%
AAGCAATACAGCTTGCCAAA+GGG + chr3_3:52624089-52624108 Msa0457600:CDS 40.0%
AAGGGTTAAAAATTCCACCG+GGG - chr3_3:52624127-52624146 None:intergenic 40.0%
!! GAGTTGGTGCAAAGTTCAAA+GGG - chr3_3:52624145-52624164 None:intergenic 40.0%
!! AGAGTTGGTGCAAAGTTCAA+AGG - chr3_3:52624146-52624165 None:intergenic 40.0%
TTTGAACTTTGCACCAACTC+TGG + chr3_3:52624145-52624164 Msa0457600:CDS 40.0%
! TTCTCTTTAGCTTCCAGAGT+TGG - chr3_3:52624161-52624180 None:intergenic 40.0%
! AACTCTGGAAGCTAAAGAGA+AGG + chr3_3:52624160-52624179 Msa0457600:CDS 40.0%
TCTTTGCTTAAATGGGACCT+GGG + chr3_3:52624242-52624261 Msa0457600:intron 40.0%
TTGTCCTGTTGGTATTGTCT+TGG + chr3_3:52624265-52624284 Msa0457600:intron 40.0%
TGTCCTGTTGGTATTGTCTT+GGG + chr3_3:52624266-52624285 Msa0457600:intron 40.0%
TTGGTATTGTCTTGGGATCT+GGG + chr3_3:52624273-52624292 Msa0457600:intron 40.0%
TCCCCAGTACCAATTTAGAT+GGG + chr3_3:52624330-52624349 Msa0457600:intron 40.0%
TCCCATCTAAATTGGTACTG+GGG - chr3_3:52624334-52624353 None:intergenic 40.0%
TCAAATGTTTGCAGGGAGAT+TGG + chr3_3:52624507-52624526 Msa0457600:intron 40.0%
TGCTGCAAGAGGTTATCAAA+TGG - chr3_3:52624586-52624605 None:intergenic 40.0%
CCCACTTAGTGTTTGAACAA+TGG - chr3_3:52624632-52624651 None:intergenic 40.0%
CTATGTACAGAGGAACTAAG+TGG + chr3_3:52624765-52624784 Msa0457600:three_prime_UTR 40.0%
AAAATGGCTTTCTAAGTGGC+GGG - chr3_3:52624913-52624932 None:intergenic 40.0%
!! GCCACTTAGAAAGCCATTTT+TGG + chr3_3:52624913-52624932 Msa0457600:three_prime_UTR 40.0%
AGAGTCTACTGAGATAATCG+TGG - chr3_3:52625013-52625032 None:intergenic 40.0%
TTTGATCCTGTGAACCAAGT+TGG - chr3_3:52625175-52625194 None:intergenic 40.0%
AGTGGCCATGTTCAACTACT+AGG - chr3_3:52622775-52622794 None:intergenic 45.0%
AGAAGCCTGTGTAGTAACAG+TGG - chr3_3:52622793-52622812 None:intergenic 45.0%
! GGCCATGTGGTTTGAAATCA+AGG + chr3_3:52622826-52622845 Msa0457600:CDS 45.0%
!! AGGTTCCTTACTGGTTCTTC+TGG + chr3_3:52622846-52622865 Msa0457600:CDS 45.0%
AGAAGTGGCTATTAGGCCAA+TGG + chr3_3:52622881-52622900 Msa0457600:CDS 45.0%
GAAGTGGCTATTAGGCCAAT+GGG + chr3_3:52622882-52622901 Msa0457600:CDS 45.0%
TGTCATACTCGCTACATCGA+TGG - chr3_3:52623142-52623161 None:intergenic 45.0%
!! ATTAGTCTTCCCGGAGACAA+TGG + chr3_3:52623774-52623793 Msa0457600:intron 45.0%
TGGATCGAATCCATTGTCTC+CGG - chr3_3:52623787-52623806 None:intergenic 45.0%
! CTTCAAGTTCTCTGGATCCT+CGG - chr3_3:52623820-52623839 None:intergenic 45.0%
AGGATCCAGAGAACTTGAAG+TGG + chr3_3:52623820-52623839 Msa0457600:CDS 45.0%
AATGGACGTTGGGCTATGTT+AGG + chr3_3:52623864-52623883 Msa0457600:CDS 45.0%
! CCAATGTTGGTGAAGACTTC+AGG - chr3_3:52623909-52623928 None:intergenic 45.0%
CCTGAAGTCTTCACCAACAT+TGG + chr3_3:52623906-52623925 Msa0457600:CDS 45.0%
GTTCCAAAATGGTACGATGC+AGG + chr3_3:52623939-52623958 Msa0457600:CDS 45.0%
! TTCCCTGCATCGTACCATTT+TGG - chr3_3:52623945-52623964 None:intergenic 45.0%
TGGCAAGACATTAAGAACCC+TGG + chr3_3:52624041-52624060 Msa0457600:CDS 45.0%
GGATCTTGGTTAACACTACC+AGG - chr3_3:52624062-52624081 None:intergenic 45.0%
CAAGCAATACAGCTTGCCAA+AGG + chr3_3:52624088-52624107 Msa0457600:CDS 45.0%
GTCTTTGCTTAAATGGGACC+TGG + chr3_3:52624241-52624260 Msa0457600:intron 45.0%
GATCCCAAGACAATACCAAC+AGG - chr3_3:52624272-52624291 None:intergenic 45.0%
GTTGGTATTGTCTTGGGATC+TGG + chr3_3:52624272-52624291 Msa0457600:intron 45.0%
CTCCCCAGTACCAATTTAGA+TGG + chr3_3:52624329-52624348 Msa0457600:intron 45.0%
GTCATTCAGCACAATGTGAC+AGG + chr3_3:52624553-52624572 Msa0457600:CDS 45.0%
CAGCACAATGTGACAGGAAA+AGG + chr3_3:52624559-52624578 Msa0457600:CDS 45.0%
!! GAACAATGGTGTTGTGCCAT+GGG - chr3_3:52624618-52624637 None:intergenic 45.0%
!! TGAACAATGGTGTTGTGCCA+TGG - chr3_3:52624619-52624638 None:intergenic 45.0%
ATCGTGGCCATTGAAAGTCA+CGG - chr3_3:52624997-52625016 None:intergenic 45.0%
TAATTTCAAGCAGACCTGCC+AGG + chr3_3:52625423-52625442 Msa0457600:three_prime_UTR 45.0%
AATTTCAAGCAGACCTGCCA+GGG + chr3_3:52625424-52625443 Msa0457600:three_prime_UTR 45.0%
! AAACCCTCTGGAAGTCACAT+TGG - chr3_3:52625451-52625470 None:intergenic 45.0%
CATGGCCACTGTTACTACAC+AGG + chr3_3:52622785-52622804 Msa0457600:exon 50.0%
!! AGGCTTCTGCAGCCATTTTC+CGG + chr3_3:52622805-52622824 Msa0457600:CDS 50.0%
CAAACCACATGGCCGGAAAA+TGG - chr3_3:52622820-52622839 None:intergenic 50.0%
GCAGAAGGTGAACAACCCAT+TGG - chr3_3:52622900-52622919 None:intergenic 50.0%
AAGAAAGGAGAGTGGTTGCC+TGG + chr3_3:52622939-52622958 Msa0457600:CDS 50.0%
GATTGACATACCTGCCAGTG+AGG - chr3_3:52622983-52623002 None:intergenic 50.0%
GGATCGAATCCATTGTCTCC+GGG - chr3_3:52623786-52623805 None:intergenic 50.0%
GTTCAAGCCGAGCTTGTGAA+TGG + chr3_3:52623846-52623865 Msa0457600:CDS 50.0%
CCAACGTCCATTCACAAGCT+CGG - chr3_3:52623856-52623875 None:intergenic 50.0%
CGAGCTTGTGAATGGACGTT+GGG + chr3_3:52623854-52623873 Msa0457600:CDS 50.0%
! TGGGCTATGTTAGGTGTTGC+AGG + chr3_3:52623873-52623892 Msa0457600:CDS 50.0%
! GGGCTATGTTAGGTGTTGCA+GGG + chr3_3:52623874-52623893 Msa0457600:CDS 50.0%
ATACAGCTTGCCAAAGGGTG+AGG + chr3_3:52624094-52624113 Msa0457600:CDS 50.0%
CAATACCAACAGGACAACCC+AGG - chr3_3:52624262-52624281 None:intergenic 50.0%
TCGAACCTGAGACCTTAAGG+AGG - chr3_3:52624307-52624326 None:intergenic 50.0%
GCATCGAACCTGAGACCTTA+AGG - chr3_3:52624310-52624329 None:intergenic 50.0%
TGTTGTGCCATGGGTCTGAA+AGG - chr3_3:52624609-52624628 None:intergenic 50.0%
AATGCTCAGTCCTAGGCATG+AGG - chr3_3:52624970-52624989 None:intergenic 50.0%
CGGTTCAAATGCTCAGTCCT+AGG - chr3_3:52624977-52624996 None:intergenic 50.0%
GGTCCAATGTGACTTCCAGA+GGG + chr3_3:52625445-52625464 Msa0457600:three_prime_UTR 50.0%
AGGAGAGTGGTTGCCTGGTT+TGG + chr3_3:52622944-52622963 Msa0457600:CDS 55.0%
TAACCAGGGGAAGCCAAACC+AGG - chr3_3:52622960-52622979 None:intergenic 55.0%
ACCTGCCAGTGAGGTAACCA+GGG - chr3_3:52622974-52622993 None:intergenic 55.0%
TACCTGCCAGTGAGGTAACC+AGG - chr3_3:52622975-52622994 None:intergenic 55.0%
CCGAGCTTGTGAATGGACGT+TGG + chr3_3:52623853-52623872 Msa0457600:CDS 55.0%
AGCTTGCCAAAGGGTGAGGT+TGG + chr3_3:52624098-52624117 Msa0457600:CDS 55.0%
GGGTAACCAACCTCACCCTT+TGG - chr3_3:52624107-52624126 None:intergenic 55.0%
! AAGGGTGAGGTTGGTTACCC+CGG + chr3_3:52624107-52624126 Msa0457600:CDS 55.0%
! TGGGAGTGTGCTCCTCCTTA+AGG + chr3_3:52624292-52624311 Msa0457600:intron 55.0%
GTGCTCCTCCTTAAGGTCTC+AGG + chr3_3:52624299-52624318 Msa0457600:intron 55.0%
!! GGTCTGAAAGGTGCTGCAAG+AGG - chr3_3:52624597-52624616 None:intergenic 55.0%
TGCAGCACCTTTCAGACCCA+TGG + chr3_3:52624599-52624618 Msa0457600:CDS 55.0%
CTCGAGACTTCCTCATGCCT+AGG + chr3_3:52624957-52624976 Msa0457600:three_prime_UTR 55.0%
AAGTCACATTGGACCCTGGC+AGG - chr3_3:52625440-52625459 None:intergenic 55.0%
CTGGAAGTCACATTGGACCC+TGG - chr3_3:52625444-52625463 None:intergenic 55.0%
GGGTCCAATGTGACTTCCAG+AGG + chr3_3:52625444-52625463 Msa0457600:three_prime_UTR 55.0%
!! GCAGCCATTTTCCGGCCATG+TGG + chr3_3:52622813-52622832 Msa0457600:CDS 60.0%
!! TTGCCTGGTTTGGCTTCCCC+TGG + chr3_3:52622954-52622973 Msa0457600:CDS 60.0%
GCTTCCCCTGGTTACCTCAC+TGG + chr3_3:52622966-52622985 Msa0457600:CDS 60.0%
CCTGCCAGTGAGGTAACCAG+GGG - chr3_3:52622973-52622992 None:intergenic 60.0%
!! CGATCCATTGGGACTAGCCG+AGG + chr3_3:52623800-52623819 Msa0457600:CDS 60.0%
! GGATCCTCGGCTAGTCCCAA+TGG - chr3_3:52623807-52623826 None:intergenic 60.0%
TGGGACCTGGGTTGTCCTGT+TGG + chr3_3:52624254-52624273 Msa0457600:intron 60.0%
CCCCTGGTTACCTCACTGGC+AGG + chr3_3:52622970-52622989 Msa0457600:CDS 65.0%
! GGTGAGGTTGGTTACCCCGG+TGG + chr3_3:52624110-52624129 Msa0457600:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 52622701 52625475 52622701 ID=Msa0457600;Name=Msa0457600
chr3_3 mRNA 52622701 52625475 52622701 ID=Msa0457600-mRNA-1;Parent=Msa0457600;Name=Msa0457600-mRNA-1;_AED=0.01;_eAED=0.01;_QI=85|1|1|1|1|1|3|815|253
chr3_3 exon 52622701 52622991 52622701 ID=Msa0457600-mRNA-1:exon:10136;Parent=Msa0457600-mRNA-1
chr3_3 exon 52623779 52624194 52623779 ID=Msa0457600-mRNA-1:exon:10137;Parent=Msa0457600-mRNA-1
chr3_3 exon 52624521 52625475 52624521 ID=Msa0457600-mRNA-1:exon:10138;Parent=Msa0457600-mRNA-1
chr3_3 five_prime_UTR 52622701 52622785 52622701 ID=Msa0457600-mRNA-1:five_prime_utr;Parent=Msa0457600-mRNA-1
chr3_3 CDS 52622786 52622991 52622786 ID=Msa0457600-mRNA-1:cds;Parent=Msa0457600-mRNA-1
chr3_3 CDS 52623779 52624194 52623779 ID=Msa0457600-mRNA-1:cds;Parent=Msa0457600-mRNA-1
chr3_3 CDS 52624521 52624660 52624521 ID=Msa0457600-mRNA-1:cds;Parent=Msa0457600-mRNA-1
chr3_3 three_prime_UTR 52624661 52625475 52624661 ID=Msa0457600-mRNA-1:three_prime_utr;Parent=Msa0457600-mRNA-1
Gene Sequence

>Msa0457600

ATGGCCACTGTTACTACACAGGCTTCTGCAGCCATTTTCCGGCCATGTGGTTTGAAATCAAGGTTCCTTACTGGTTCTTCTGGTAAGTTGAACAGAGAAGTGGCTATTAGGCCAATGGGTTGTTCACCTTCTGCTTCTTTTAAGGTTGAAGCTAAGAAAGGAGAGTGGTTGCCTGGTTTGGCTTCCCCTGGTTACCTCACTGGCAGTCTTCCCGGAGACAATGGATTCGATCCATTGGGACTAGCCGAGGATCCAGAGAACTTGAAGTGGTTTGTTCAAGCCGAGCTTGTGAATGGACGTTGGGCTATGTTAGGTGTTGCAGGGATGTTACTCCCTGAAGTCTTCACCAACATTGGAATCATCAACGTTCCAAAATGGTACGATGCAGGGAAAGAAGAATATTTTGCTTCATCTTCAACACTCTTTGTGATCGAGTTCATTTTGTTCCATTACGTCGAGATTAGAAGATGGCAAGACATTAAGAACCCTGGTAGTGTTAACCAAGATCCTATCTTCAAGCAATACAGCTTGCCAAAGGGTGAGGTTGGTTACCCCGGTGGAATTTTTAACCCTTTGAACTTTGCACCAACTCTGGAAGCTAAAGAGAAGGAAATTGCTAATGGGAGATTGGCAATGTTAGCATTCTTGGGATTTGTCATTCAGCACAATGTGACAGGAAAAGGACCATTTGATAACCTCTTGCAGCACCTTTCAGACCCATGGCACAACACCATTGTTCAAACACTAAGTGGGGGCAACTAA
Protein sequence

>Msa0457600

MATVTTQASAAIFRPCGLKSRFLTGSSGKLNREVAIRPMGCSPSASFKVEAKKGEWLPGLASPGYLTGSLPGDNGFDPLGLAEDPENLKWFVQAELVNGRWAMLGVAGMLLPEVFTNIGIINVPKWYDAGKEEYFASSSTLFVIEFILFHYVEIRRWQDIKNPGSVNQDPIFKQYSLPKGEVGYPGGIFNPLNFAPTLEAKEKEIANGRLAMLAFLGFVIQHNVTGKGPFDNLLQHLSDPWHNTIVQTLSGGN*