AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32652


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32652.t1 MTR_3g106820 98.780 164 2 0 1 164 46 209 4.24e-113 320
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32652.t1 AT4G32260 78.431 153 31 1 11 161 66 218 6.95e-70 211

Find 34 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAGAAGCTCTTGCAAATT+TGG 0.138611 3.1:-84638308 MS.gene32652:CDS
TTCCAACTCCTTCACTTCTT+CGG 0.354892 3.1:+84638462 None:intergenic
AGAAATTCAACGACTATGAT+TGG 0.371223 3.1:+84638592 None:intergenic
CACCCCTCTCGGAACCTTCA+TGG 0.382299 3.1:-84638536 MS.gene32652:CDS
GAACCTTGTTAACAATATCT+TGG 0.384952 3.1:+84638230 None:intergenic
TTGAGATATCAACGAGAAAC+AGG 0.396683 3.1:+84638205 None:intergenic
TGCAAATTTGGAAAAGCAAA+AGG 0.407369 3.1:-84638296 MS.gene32652:CDS
CGCAGCTCTCAAAACTGCAT+TGG 0.476740 3.1:+84638432 None:intergenic
GAAATTCAACGACTATGATT+GGG 0.478683 3.1:+84638593 None:intergenic
CGCGTCACGATTGTCCATGA+AGG 0.481050 3.1:+84638522 None:intergenic
CTTCACTTCTTCGGATGTGT+TGG 0.483273 3.1:+84638471 None:intergenic
AAACTCTACTTCACCCCTCT+CGG 0.522559 3.1:-84638547 MS.gene32652:CDS
AGAGAGGGAAGTTGCTGCTA+GGG 0.528221 3.1:+84638681 None:intergenic
AAGAGAGGGAAGTTGCTGCT+AGG 0.534616 3.1:+84638680 None:intergenic
TAGCCAAGATATTGTTAACA+AGG 0.566254 3.1:-84638233 MS.gene32652:CDS
AGCGAGGGAAGGTGGTGCGA+AGG 0.566516 3.1:+84638654 None:intergenic
ATCCGAAGAAGTGAAGGAGT+TGG 0.580997 3.1:-84638464 MS.gene32652:CDS
TGCGCGTGCTGAAATTGCGG+TGG 0.591536 3.1:-84638413 MS.gene32652:CDS
AAGCGAAGATTGCGGAAGGT+AGG 0.592242 3.1:-84638351 MS.gene32652:CDS
TGGTGCGAAGGCGAAAGAGA+GGG 0.595311 3.1:+84638666 None:intergenic
CGACTATGATTGGGAGAGTG+AGG 0.595912 3.1:+84638602 None:intergenic
CAACACATCCGAAGAAGTGA+AGG 0.596455 3.1:-84638470 MS.gene32652:CDS
AGCTGCGCGTGCTGAAATTG+CGG 0.598150 3.1:-84638416 MS.gene32652:CDS
GTTTGTCGAGAGCGAACATG+AGG 0.598866 3.1:+84638566 None:intergenic
GTGGTGCGAAGGCGAAAGAG+AGG 0.606516 3.1:+84638665 None:intergenic
GTCCATGAAGGTTCCGAGAG+GGG 0.611279 3.1:+84638534 None:intergenic
TGTCCATGAAGGTTCCGAGA+GGG 0.615991 3.1:+84638533 None:intergenic
AATCGTGACGCGGAAATCAG+AGG 0.616507 3.1:-84638511 MS.gene32652:CDS
GATTGCGGAAGGTAGGAAGA+AGG 0.621119 3.1:-84638344 MS.gene32652:CDS
GTTGAAGCGAAGATTGCGGA+AGG 0.664257 3.1:-84638355 MS.gene32652:CDS
GAGGTTGAAATCGAAGAGTG+CGG 0.686862 3.1:+84638621 None:intergenic
TGAAGTTGAAGCGAAGATTG+CGG 0.692066 3.1:-84638359 MS.gene32652:CDS
CTTCATGGACAATCGTGACG+CGG 0.701097 3.1:-84638521 MS.gene32652:CDS
TTGTCCATGAAGGTTCCGAG+AGG 0.735316 3.1:+84638532 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGAAATTCAACGACTATGAT+TGG + chr3.1:84638309-84638328 None:intergenic 30.0%
GAAATTCAACGACTATGATT+GGG + chr3.1:84638308-84638327 None:intergenic 30.0%
GAACCTTGTTAACAATATCT+TGG + chr3.1:84638671-84638690 None:intergenic 30.0%
TAGCCAAGATATTGTTAACA+AGG - chr3.1:84638665-84638684 MS.gene32652:CDS 30.0%
TGCAAATTTGGAAAAGCAAA+AGG - chr3.1:84638602-84638621 MS.gene32652:CDS 30.0%
GCAAGAAGCTCTTGCAAATT+TGG - chr3.1:84638590-84638609 MS.gene32652:CDS 40.0%
TGAAGTTGAAGCGAAGATTG+CGG - chr3.1:84638539-84638558 MS.gene32652:CDS 40.0%
TTCCAACTCCTTCACTTCTT+CGG + chr3.1:84638439-84638458 None:intergenic 40.0%
AAACTCTACTTCACCCCTCT+CGG - chr3.1:84638351-84638370 MS.gene32652:CDS 45.0%
ATCCGAAGAAGTGAAGGAGT+TGG - chr3.1:84638434-84638453 MS.gene32652:CDS 45.0%
CAACACATCCGAAGAAGTGA+AGG - chr3.1:84638428-84638447 MS.gene32652:CDS 45.0%
CTTCACTTCTTCGGATGTGT+TGG + chr3.1:84638430-84638449 None:intergenic 45.0%
GAGGTTGAAATCGAAGAGTG+CGG + chr3.1:84638280-84638299 None:intergenic 45.0%
AAGAGAGGGAAGTTGCTGCT+AGG + chr3.1:84638221-84638240 None:intergenic 50.0%
AAGCGAAGATTGCGGAAGGT+AGG - chr3.1:84638547-84638566 MS.gene32652:CDS 50.0%
AATCGTGACGCGGAAATCAG+AGG - chr3.1:84638387-84638406 MS.gene32652:CDS 50.0%
AGAGAGGGAAGTTGCTGCTA+GGG + chr3.1:84638220-84638239 None:intergenic 50.0%
CGACTATGATTGGGAGAGTG+AGG + chr3.1:84638299-84638318 None:intergenic 50.0%
CGCAGCTCTCAAAACTGCAT+TGG + chr3.1:84638469-84638488 None:intergenic 50.0%
CTTCATGGACAATCGTGACG+CGG - chr3.1:84638377-84638396 MS.gene32652:CDS 50.0%
GATTGCGGAAGGTAGGAAGA+AGG - chr3.1:84638554-84638573 MS.gene32652:CDS 50.0%
GTTGAAGCGAAGATTGCGGA+AGG - chr3.1:84638543-84638562 MS.gene32652:CDS 50.0%
GTTTGTCGAGAGCGAACATG+AGG + chr3.1:84638335-84638354 None:intergenic 50.0%
TGTCCATGAAGGTTCCGAGA+GGG + chr3.1:84638368-84638387 None:intergenic 50.0%
TTGTCCATGAAGGTTCCGAG+AGG + chr3.1:84638369-84638388 None:intergenic 50.0%
! TTTTCGAAAGCGAGGGAAGG+TGG + chr3.1:84638255-84638274 None:intergenic 50.0%
!!! GCTTTTTCGAAAGCGAGGGA+AGG + chr3.1:84638258-84638277 None:intergenic 50.0%
!!! TGCGGCTTTTTCGAAAGCGA+GGG + chr3.1:84638262-84638281 None:intergenic 50.0%
AGCTGCGCGTGCTGAAATTG+CGG - chr3.1:84638482-84638501 MS.gene32652:CDS 55.0%
CGCGTCACGATTGTCCATGA+AGG + chr3.1:84638379-84638398 None:intergenic 55.0%
GTCCATGAAGGTTCCGAGAG+GGG + chr3.1:84638367-84638386 None:intergenic 55.0%
TGGTGCGAAGGCGAAAGAGA+GGG + chr3.1:84638235-84638254 None:intergenic 55.0%
!!! GTGCGGCTTTTTCGAAAGCG+AGG + chr3.1:84638263-84638282 None:intergenic 55.0%
CACCCCTCTCGGAACCTTCA+TGG - chr3.1:84638362-84638381 MS.gene32652:CDS 60.0%
GTGGTGCGAAGGCGAAAGAG+AGG + chr3.1:84638236-84638255 None:intergenic 60.0%
TGCGCGTGCTGAAATTGCGG+TGG - chr3.1:84638485-84638504 MS.gene32652:CDS 60.0%
AGCGAGGGAAGGTGGTGCGA+AGG + chr3.1:84638247-84638266 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 84638213 84638707 84638213 ID=MS.gene32652
chr3.1 mRNA 84638213 84638707 84638213 ID=MS.gene32652.t1;Parent=MS.gene32652
chr3.1 exon 84638213 84638707 84638213 ID=MS.gene32652.t1.exon1;Parent=MS.gene32652.t1
chr3.1 CDS 84638213 84638707 84638213 ID=cds.MS.gene32652.t1;Parent=MS.gene32652.t1
Gene Sequence

>MS.gene32652

ATGACCCTAGCAGCAACTTCCCTCTCTTTCGCCTTCGCACCACCTTCCCTCGCTTTCGAAAAAGCCGCACTCTTCGATTTCAACCTCACTCTCCCAATCATAGTCGTTGAATTTCTCTTCCTCATGTTCGCTCTCGACAAACTCTACTTCACCCCTCTCGGAACCTTCATGGACAATCGTGACGCGGAAATCAGAGGCAAACTCAACAGCGTCACCAACACATCCGAAGAAGTGAAGGAGTTGGAAGAACAAGCCAATGCAGTTTTGAGAGCTGCGCGTGCTGAAATTGCGGTGGCGCTGAATCAGATGAAGAAAGAGACTCAAGCTGAAGTTGAAGCGAAGATTGCGGAAGGTAGGAAGAAGGTTGATGCTGAATTGCAAGAAGCTCTTGCAAATTTGGAAAAGCAAAAGGAAGAAACTGTTAAAGCTCTTGATTCTCAGATTGCTGCTCTTAGCCAAGATATTGTTAACAAGGTTCTTCCTGTTTCTCGTTGA

Protein sequence

>MS.gene32652.t1

MTLAATSLSFAFAPPSLAFEKAALFDFNLTLPIIVVEFLFLMFALDKLYFTPLGTFMDNRDAEIRGKLNSVTNTSEEVKELEEQANAVLRAARAEIAVALNQMKKETQAEVEAKIAEGRKKVDAELQEALANLEKQKEETVKALDSQIAALSQDIVNKVLPVSR