AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07302

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07302.t1 MTR_1g086940 95.812 191 6 1 1 191 1 189 2.83e-131 371
MS.gene07302.t1 MTR_7g109610 74.332 187 40 4 10 191 3 186 1.65e-88 262
MS.gene07302.t1 MTR_7g082980 43.363 113 51 2 80 191 57 157 7.95e-26 101
MS.gene07302.t1 MTR_4g075100 49.398 83 42 0 109 191 41 123 1.22e-23 94.4
MS.gene07302.t1 MTR_5g026840 49.367 79 40 0 118 196 93 171 3.55e-20 86.3
MS.gene07302.t1 MTR_4g034690 36.792 106 59 1 86 191 3 100 5.76e-19 80.9
MS.gene07302.t1 MTR_4g069800 48.684 76 39 0 116 191 102 177 6.35e-18 79.3
MS.gene07302.t1 MTR_4g069800 48.684 76 39 0 116 191 102 177 9.77e-18 79.3
MS.gene07302.t1 MTR_4g102000 30.556 180 92 6 11 190 61 207 1.54e-16 76.6
MS.gene07302.t1 MTR_4g069800 48.571 70 36 0 122 191 76 145 2.07e-16 75.1
MS.gene07302.t1 MTR_0002s0730 32.432 111 67 1 86 196 3 105 1.82e-14 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07302.t1 AT1G02560 72.396 192 52 1 1 191 1 192 7.55e-93 273
MS.gene07302.t1 AT5G23140 51.136 88 43 0 109 196 44 131 2.62e-25 99.0
MS.gene07302.t1 AT1G66670 53.947 76 35 0 116 191 88 163 3.38e-23 94.7
MS.gene07302.t1 AT5G45390 49.398 83 42 0 114 196 80 162 5.99e-21 88.2
MS.gene07302.t1 ATCG00670 38.679 106 57 2 86 191 3 100 1.04e-20 85.9
MS.gene07302.t1 AT1G12410 41.558 77 45 0 114 190 90 166 7.76e-17 77.0
MS.gene07302.t1 AT1G11750 33.333 177 105 5 20 191 6 174 7.41e-16 74.3
MS.gene07302.t1 AT1G11750 31.771 192 106 5 20 191 6 192 2.01e-14 70.5

Find 58 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGTGTATGGTGATCAATTT+TGG 0.210761 1.2:-65322981 MS.gene07302:CDS
GTGTTCTTCTAGGGAGGATT+TGG 0.266446 1.2:-65322852 MS.gene07302:intron
GGAGTTCACGTCAGGGTATT+TGG 0.314629 1.2:-65322948 MS.gene07302:CDS
AACGACGGACAAACCTTGTT+TGG 0.358156 1.2:+65322550 None:intergenic
GATCAACAGCATCAAGATAA+AGG 0.360915 1.2:+65322572 None:intergenic
TGTTCTTCTAGGGAGGATTT+GGG 0.362409 1.2:-65322851 MS.gene07302:intron
TGTGCATAGGTAGGAAAATA+AGG 0.368409 1.2:+65322815 None:intergenic
CCTTCGAACTCTTTGCTCTC+CGG 0.369539 1.2:-65323714 MS.gene07302:CDS
GGAAACGCTCCTGCACCATT+GGG 0.370261 1.2:+65322777 None:intergenic
TGGAAACGCTCCTGCACCAT+TGG 0.372062 1.2:+65322776 None:intergenic
CTTCGAACTCTTTGCTCTCC+GGG 0.395033 1.2:-65323713 MS.gene07302:intron
ACCAGCTGTAACTGATCCTC+CGG 0.426032 1.2:+65322363 None:intergenic
ACCCGAGAGGAGTTCACGTC+AGG 0.435644 1.2:-65322956 MS.gene07302:CDS
ATCATATAACCACACGTGAT+TGG 0.439063 1.2:+65321704 None:intergenic
AGGAGAGTTGAGTGTAAGAG+AGG 0.441042 1.2:+65323788 None:intergenic
GTTGAGTGTAAGAGAGGAAG+AGG 0.452445 1.2:+65323794 None:intergenic
ATCACCATACACAGCTTTCA+CGG 0.458968 1.2:+65322990 None:intergenic
TTGAGTGTAAGAGAGGAAGA+GGG 0.464850 1.2:+65323795 None:intergenic
GTCAGGGTATTTGGTCCATA+AGG 0.486578 1.2:-65322939 MS.gene07302:intron
CTGTGATTTGCAGGAAATTG+AGG 0.490449 1.2:-65323050 MS.gene07302:intron
ACCCTGACGTGAACTCCTCT+CGG 0.491382 1.2:+65322954 None:intergenic
TGGCCCTTGTCCTTGTGCAT+AGG 0.495346 1.2:+65322802 None:intergenic
CACTGTCTGTGTTGGATTGG+CGG 0.498037 1.2:-65322165 MS.gene07302:CDS
GTGAGAGTGAGAAGAAGAAC+CGG 0.507930 1.2:+65323749 None:intergenic
AAACGCTCCTGCACCATTGG+GGG 0.517765 1.2:+65322779 None:intergenic
GTCCACTGTCTGTGTTGGAT+TGG 0.520930 1.2:-65322168 MS.gene07302:CDS
TGGAGCAGTTGACGATGATA+TGG 0.521009 1.2:-65322617 MS.gene07302:CDS
ACACAGACAGTGGACACATC+AGG 0.523363 1.2:+65322176 None:intergenic
GAAACGCTCCTGCACCATTG+GGG 0.523580 1.2:+65322778 None:intergenic
TCTGTGTTGGATTGGCGGCT+AGG 0.523757 1.2:-65322160 MS.gene07302:intron
GATGTGTCCACTGTCTGTGT+TGG 0.530369 1.2:-65322173 MS.gene07302:CDS
TGAGTGTAAGAGAGGAAGAG+GGG 0.531079 1.2:+65323796 None:intergenic
AGAAGAAGAACCGGTGGTGG+GGG 0.535745 1.2:+65323758 None:intergenic
CCCGGAGGATCAGTTACAGC+TGG 0.538507 1.2:-65322364 MS.gene07302:intron
CCTACCTATGCACAAGGACA+AGG 0.541580 1.2:-65322806 MS.gene07302:CDS
GTGAGAAGAAGAACCGGTGG+TGG 0.542322 1.2:+65323755 None:intergenic
GTCATGTATGTAAATTCTCC+CGG 0.542326 1.2:-65322382 MS.gene07302:CDS
AAAGTTGACTTATAACACTC+TGG 0.552761 1.2:+65322756 None:intergenic
GAGTGTAAGAGAGGAAGAGG+GGG 0.557336 1.2:+65323797 None:intergenic
GTAGGAAAATAAGGCGAAGA+CGG 0.567411 1.2:+65322824 None:intergenic
CCCTGACGTGAACTCCTCTC+GGG 0.567797 1.2:+65322955 None:intergenic
TGAGAAGAAGAACCGGTGGT+GGG 0.577299 1.2:+65323756 None:intergenic
TCTGCCGTGAAAGCTGTGTA+TGG 0.585170 1.2:-65322994 MS.gene07302:CDS
CCTTGTCCTTGTGCATAGGT+AGG 0.585374 1.2:+65322806 None:intergenic
GAGAAGAAGAACCGGTGGTG+GGG 0.592581 1.2:+65323757 None:intergenic
TAGAGAATAATCCGTTGTGG+TGG 0.606980 1.2:-65322637 MS.gene07302:CDS
TGATGCTGTTGATCCAAACA+AGG 0.607848 1.2:-65322563 MS.gene07302:intron
CCCGAGAGGAGTTCACGTCA+GGG 0.610211 1.2:-65322955 MS.gene07302:CDS
CCGGAGAGCAAAGAGTTCGA+AGG 0.611003 1.2:+65323714 None:intergenic
CCAGCTGTAACTGATCCTCC+GGG 0.618930 1.2:+65322364 None:intergenic
CGCCAATCCAACACAGACAG+TGG 0.626024 1.2:+65322166 None:intergenic
TGGCAATATTTGATACAATG+AGG 0.631433 1.2:-65322208 MS.gene07302:CDS
ATTTAGAGAATAATCCGTTG+TGG 0.633851 1.2:-65322640 MS.gene07302:intron
CTACCTATGCACAAGGACAA+GGG 0.671421 1.2:-65322805 MS.gene07302:CDS
CGTCAACTGCTCCACCACAA+CGG 0.680926 1.2:+65322626 None:intergenic
TAGTCTCAACCAATCACGTG+TGG 0.699122 1.2:-65321713 None:intergenic
ATGTATGTAAATTCTCCCGG+AGG 0.710686 1.2:-65322379 MS.gene07302:CDS
AGAGTGAGAAGAAGAACCGG+TGG 0.741682 1.2:+65323752 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATTATCTAAAAAAAGAT+AGG + chr1.2:65323590-65323609 None:intergenic 10.0%
!!! ATGAACAAAATTTAAAATAT+AGG - chr1.2:65323758-65323777 MS.gene07302:CDS 10.0%
!! ATATTATGATAAATTTGAAC+TGG - chr1.2:65322171-65322190 MS.gene07302:CDS 15.0%
!! TAAAATTGAGTTAAAAGAAA+AGG + chr1.2:65321918-65321937 None:intergenic 15.0%
!!! TTATATTTTACTTCTACTAA+GGG - chr1.2:65322448-65322467 MS.gene07302:intron 15.0%
!!! TTTATATTTTACTTCTACTA+AGG - chr1.2:65322447-65322466 MS.gene07302:intron 15.0%
!! AAACATAGAAAATGACAAAA+GGG + chr1.2:65323667-65323686 None:intergenic 20.0%
!! AAATAGCATAGAATATTCTA+CGG + chr1.2:65322427-65322446 None:intergenic 20.0%
!! AATAGATTATCAATAATCGA+AGG + chr1.2:65322868-65322887 None:intergenic 20.0%
!! AGTAAAAAATCCTTACATAT+TGG + chr1.2:65322804-65322823 None:intergenic 20.0%
!! ATAAAAATAAGAACTTTACC+AGG + chr1.2:65321944-65321963 None:intergenic 20.0%
!! CTACTAGCAAAATTTAAAAA+GGG + chr1.2:65322079-65322098 None:intergenic 20.0%
!! TAAAAAAAGATAGGCTTAAT+AGG + chr1.2:65323581-65323600 None:intergenic 20.0%
!! TAATATTTCATTCTTCTTGT+GGG - chr1.2:65323396-65323415 MS.gene07302:intron 20.0%
!! TACTAGCAAAATTTAAAAAG+GGG + chr1.2:65322078-65322097 None:intergenic 20.0%
!!! AATTTAAAATATAGGATACC+AGG - chr1.2:65323766-65323785 MS.gene07302:CDS 20.0%
!!! CTTTTTAAATTTTGCTAGTA+GGG - chr1.2:65322078-65322097 MS.gene07302:intron 20.0%
!!! TTTAACTCAATTTTAATTCC+TGG - chr1.2:65321923-65321942 MS.gene07302:intron 20.0%
!!! TTTACTTGTTTCTTTGTTTT+GGG - chr1.2:65322030-65322049 MS.gene07302:intron 20.0%
! AACATAGAAAATGACAAAAG+GGG + chr1.2:65323666-65323685 None:intergenic 25.0%
! AATAGCATAGAATATTCTAC+GGG + chr1.2:65322426-65322445 None:intergenic 25.0%
! ATTACATGTAGTCTAAACAT+AGG - chr1.2:65323242-65323261 MS.gene07302:intron 25.0%
! CCTACTAGCAAAATTTAAAA+AGG + chr1.2:65322080-65322099 None:intergenic 25.0%
! GAAACATAGAAAATGACAAA+AGG + chr1.2:65323668-65323687 None:intergenic 25.0%
! GAAAGAAAAATATACATACC+CGG + chr1.2:65321841-65321860 None:intergenic 25.0%
! GGATCACTTTATATAGATAA+AGG + chr1.2:65323041-65323060 None:intergenic 25.0%
! GTAATATTTCATTCTTCTTG+TGG - chr1.2:65323395-65323414 MS.gene07302:intron 25.0%
! TTGAAATTGAAGAAAAGACA+AGG + chr1.2:65321863-65321882 None:intergenic 25.0%
! TTGAGACAAATGAATAATCT+GGG - chr1.2:65322201-65322220 MS.gene07302:CDS 25.0%
! TTGAGTTAAAAGAAAAGGTT+AGG + chr1.2:65321913-65321932 None:intergenic 25.0%
! TTGATATATGTGTTCTTCTA+GGG - chr1.2:65322672-65322691 MS.gene07302:intron 25.0%
! TTTGATCAACTAAAAAACGA+CGG + chr1.2:65323001-65323020 None:intergenic 25.0%
! TTTGTGTGTACATGAAATTT+AGG + chr1.2:65323482-65323501 None:intergenic 25.0%
!! CCTTTTTAAATTTTGCTAGT+AGG - chr1.2:65322077-65322096 MS.gene07302:intron 25.0%
!!! ATGTAAGGATTTTTTACTCT+TGG - chr1.2:65322806-65322825 MS.gene07302:CDS 25.0%
!!! CTTTCTTTGTTTTTGTTCTT+AGG - chr1.2:65323300-65323319 MS.gene07302:intron 25.0%
!!! GTTTACTTGTTTCTTTGTTT+TGG - chr1.2:65322029-65322048 MS.gene07302:intron 25.0%
!!! TTTCTTTGTTTTGGGTTATT+AGG - chr1.2:65322038-65322057 MS.gene07302:intron 25.0%
!!! TTTGTTTTTGTTCTTAGGTA+TGG - chr1.2:65323305-65323324 MS.gene07302:intron 25.0%
!!! TTTTTTTCCTTCCAATTTGT+GGG - chr1.2:65321987-65322006 MS.gene07302:intron 25.0%
!!! TTTTTTTTCCTTCCAATTTG+TGG - chr1.2:65321986-65322005 MS.gene07302:intron 25.0%
AAAACTCAATACCCACAAAT+TGG + chr1.2:65322001-65322020 None:intergenic 30.0%
AAAGGTTAGGAATAAGTTCA+AGG + chr1.2:65321900-65321919 None:intergenic 30.0%
AAGGTTAGGAATAAGTTCAA+GGG + chr1.2:65321899-65321918 None:intergenic 30.0%
ACAAGCAAAAATCTACCTTA+TGG + chr1.2:65322612-65322631 None:intergenic 30.0%
ACTAGAGCTAATTAATCACT+TGG + chr1.2:65322273-65322292 None:intergenic 30.0%
ATTTAGAGAATAATCCGTTG+TGG - chr1.2:65322893-65322912 MS.gene07302:intron 30.0%
CTTGAGACAAATGAATAATC+TGG - chr1.2:65322200-65322219 MS.gene07302:CDS 30.0%
CTTGATATATGTGTTCTTCT+AGG - chr1.2:65322671-65322690 MS.gene07302:intron 30.0%
TCTTATCTACTATGTTGAAC+AGG - chr1.2:65323123-65323142 MS.gene07302:intron 30.0%
TGGCAATATTTGATACAATG+AGG - chr1.2:65323325-65323344 MS.gene07302:intron 30.0%
! AAAGTTGACTTATAACACTC+TGG + chr1.2:65322780-65322799 None:intergenic 30.0%
! AAATTTTGCTAGTAGGGTTT+GGG - chr1.2:65322084-65322103 MS.gene07302:intron 30.0%
! GTCAACTTTTCCAATATGTA+AGG - chr1.2:65322791-65322810 MS.gene07302:CDS 30.0%
! TAAATTTTGCTAGTAGGGTT+TGG - chr1.2:65322083-65322102 MS.gene07302:intron 30.0%
! TGTGTATGGTGATCAATTTT+GGG - chr1.2:65322553-65322572 MS.gene07302:intron 30.0%
!! CAAACACATGCATTTTGATA+TGG - chr1.2:65323083-65323102 MS.gene07302:intron 30.0%
!! GTGATTAATTAGCTCTAGTT+AGG - chr1.2:65322274-65322293 MS.gene07302:intron 30.0%
!!! TTTTTGTTTTGCTGAGTTCT+AGG - chr1.2:65323514-65323533 MS.gene07302:intron 30.0%
ACCACTCACTATCAGTAAAA+GGG - chr1.2:65323445-65323464 MS.gene07302:intron 35.0%
ACCAGAAAGTAAGTAAAGTC+TGG + chr1.2:65322352-65322371 None:intergenic 35.0%
ATAGAATATTCTACGGGAAG+GGG + chr1.2:65322420-65322439 None:intergenic 35.0%
CATAGAATATTCTACGGGAA+GGG + chr1.2:65322421-65322440 None:intergenic 35.0%
GACAAATGAATAATCTGGGT+TGG - chr1.2:65322205-65322224 MS.gene07302:CDS 35.0%
GATCAACAGCATCAAGATAA+AGG + chr1.2:65322964-65322983 None:intergenic 35.0%
GTCATGTATGTAAATTCTCC+CGG - chr1.2:65323151-65323170 MS.gene07302:intron 35.0%
TACCACTCACTATCAGTAAA+AGG - chr1.2:65323444-65323463 MS.gene07302:intron 35.0%
TGTGCATAGGTAGGAAAATA+AGG + chr1.2:65322721-65322740 None:intergenic 35.0%
TTTCATAGAATACTAGCGAC+AGG + chr1.2:65323690-65323709 None:intergenic 35.0%
! ATATATGTGTTCTTCTAGGG+AGG - chr1.2:65322675-65322694 MS.gene07302:intron 35.0%
! CTGTGTATGGTGATCAATTT+TGG - chr1.2:65322552-65322571 MS.gene07302:intron 35.0%
! GAGAATTTGAGTTCAAATCG+TGG - chr1.2:65322310-65322329 MS.gene07302:intron 35.0%
! GTGTATGGTGATCAATTTTG+GGG - chr1.2:65322554-65322573 MS.gene07302:intron 35.0%
! TATTTTCCTACCTATGCACA+AGG - chr1.2:65322721-65322740 MS.gene07302:intron 35.0%
! TGACATCTCTAGAAATGCAA+AGG + chr1.2:65323062-65323081 None:intergenic 35.0%
!! TTTTAGTTGATCAAAGCTCG+TGG - chr1.2:65323006-65323025 MS.gene07302:CDS 35.0%
ATCACCATACACAGCTTTCA+CGG + chr1.2:65322546-65322565 None:intergenic 40.0%
ATGAAACCCCGACATAGATA+GGG - chr1.2:65323704-65323723 MS.gene07302:intron 40.0%
ATGTATGTAAATTCTCCCGG+AGG - chr1.2:65323154-65323173 MS.gene07302:intron 40.0%
ATTTCCTGCAAATCACAGCA+TGG + chr1.2:65322481-65322500 None:intergenic 40.0%
CTCAATACCCACAAATTGGA+AGG + chr1.2:65321997-65322016 None:intergenic 40.0%
GCATAGAATATTCTACGGGA+AGG + chr1.2:65322422-65322441 None:intergenic 40.0%
GCCAGACTTTACTTACTTTC+TGG - chr1.2:65322348-65322367 MS.gene07302:intron 40.0%
GTAGGAAAATAAGGCGAAGA+CGG + chr1.2:65322712-65322731 None:intergenic 40.0%
TAGAATATTCTACGGGAAGG+GGG + chr1.2:65322419-65322438 None:intergenic 40.0%
TAGAGAATAATCCGTTGTGG+TGG - chr1.2:65322896-65322915 MS.gene07302:intron 40.0%
TATGAAACCCCGACATAGAT+AGG - chr1.2:65323703-65323722 MS.gene07302:intron 40.0%
TCTCAAGTCTCAACAAATCC+TGG + chr1.2:65323787-65323806 None:intergenic 40.0%
TGATGCTGTTGATCCAAACA+AGG - chr1.2:65322970-65322989 MS.gene07302:CDS 40.0%
TTGAGACTTGAGATCATGAG+AGG - chr1.2:65323794-65323813 MS.gene07302:CDS 40.0%
TTGAGTGTAAGAGAGGAAGA+GGG + chr1.2:65321741-65321760 None:intergenic 40.0%
! AAAACCATGCTGTGATTTGC+AGG - chr1.2:65322474-65322493 MS.gene07302:intron 40.0%
! CTGTGATTTGCAGGAAATTG+AGG - chr1.2:65322483-65322502 MS.gene07302:intron 40.0%
! TGTTCTTCTAGGGAGGATTT+GGG - chr1.2:65322682-65322701 MS.gene07302:intron 40.0%
!! ACCCTTTTACTGATAGTGAG+TGG + chr1.2:65323449-65323468 None:intergenic 40.0%
AACGACGGACAAACCTTGTT+TGG + chr1.2:65322986-65323005 None:intergenic 45.0%
AATAGGCAGTGTGCAAGTGA+GGG + chr1.2:65323564-65323583 None:intergenic 45.0%
AGGAGAGTTGAGTGTAAGAG+AGG + chr1.2:65321748-65321767 None:intergenic 45.0%
CTACCTATGCACAAGGACAA+GGG - chr1.2:65322728-65322747 MS.gene07302:intron 45.0%
CTGTATCCCCCTATCTATGT+CGG + chr1.2:65323715-65323734 None:intergenic 45.0%
GGTTGAACTCCAAATTACCG+AGG - chr1.2:65322369-65322388 MS.gene07302:CDS 45.0%
GTGAGAGTGAGAAGAAGAAC+CGG + chr1.2:65321787-65321806 None:intergenic 45.0%
GTTGAGTGTAAGAGAGGAAG+AGG + chr1.2:65321742-65321761 None:intergenic 45.0%
TAATAGGCAGTGTGCAAGTG+AGG + chr1.2:65323565-65323584 None:intergenic 45.0%
TGAAACCCCGACATAGATAG+GGG - chr1.2:65323705-65323724 MS.gene07302:intron 45.0%
TGAGTGTAAGAGAGGAAGAG+GGG + chr1.2:65321740-65321759 None:intergenic 45.0%
TGGAGCAGTTGACGATGATA+TGG - chr1.2:65322916-65322935 MS.gene07302:intron 45.0%
TGTATCCCCCTATCTATGTC+GGG + chr1.2:65323714-65323733 None:intergenic 45.0%
! AGCTTTCACGGCAGATTTTC+GGG + chr1.2:65322534-65322553 None:intergenic 45.0%
! CAGCTTTCACGGCAGATTTT+CGG + chr1.2:65322535-65322554 None:intergenic 45.0%
! GACAAAAGGGGTGTGTATGA+GGG + chr1.2:65323654-65323673 None:intergenic 45.0%
! GTCAGGGTATTTGGTCCATA+AGG - chr1.2:65322594-65322613 MS.gene07302:CDS 45.0%
! GTGTTCTTCTAGGGAGGATT+TGG - chr1.2:65322681-65322700 MS.gene07302:intron 45.0%
! TGACAAAAGGGGTGTGTATG+AGG + chr1.2:65323655-65323674 None:intergenic 45.0%
ACACAGACAGTGGACACATC+AGG + chr1.2:65323360-65323379 None:intergenic 50.0%
ACCAGCTGTAACTGATCCTC+CGG + chr1.2:65323173-65323192 None:intergenic 50.0%
AGAGTGAGAAGAAGAACCGG+TGG + chr1.2:65321784-65321803 None:intergenic 50.0%
ATTACCGAGGTTTCCTCCCT+CGG - chr1.2:65322382-65322401 MS.gene07302:CDS 50.0%
CCTACCTATGCACAAGGACA+AGG - chr1.2:65322727-65322746 MS.gene07302:intron 50.0%
CCTTCGAACTCTTTGCTCTC+CGG - chr1.2:65321819-65321838 MS.gene07302:intron 50.0%
CCTTGTCCTTGTGCATAGGT+AGG + chr1.2:65322730-65322749 None:intergenic 50.0%
CGACATAGATAGGGGGATAC+AGG - chr1.2:65323713-65323732 MS.gene07302:intron 50.0%
CTTCGAACTCTTTGCTCTCC+GGG - chr1.2:65321820-65321839 MS.gene07302:intron 50.0%
GAAACCCCGACATAGATAGG+GGG - chr1.2:65323706-65323725 MS.gene07302:intron 50.0%
GAGTGTAAGAGAGGAAGAGG+GGG + chr1.2:65321739-65321758 None:intergenic 50.0%
GATGTGTCCACTGTCTGTGT+TGG - chr1.2:65323360-65323379 MS.gene07302:intron 50.0%
GGGAGGAAACCTCGGTAATT+TGG + chr1.2:65322381-65322400 None:intergenic 50.0%
GTATCCCCCTATCTATGTCG+GGG + chr1.2:65323713-65323732 None:intergenic 50.0%
GTCCACTGTCTGTGTTGGAT+TGG - chr1.2:65323365-65323384 MS.gene07302:intron 50.0%
TCTGCCGTGAAAGCTGTGTA+TGG - chr1.2:65322539-65322558 MS.gene07302:intron 50.0%
TGAGAAGAAGAACCGGTGGT+GGG + chr1.2:65321780-65321799 None:intergenic 50.0%
! CACTGTCTGTGTTGGATTGG+CGG - chr1.2:65323368-65323387 MS.gene07302:intron 50.0%
! GGAGTTCACGTCAGGGTATT+TGG - chr1.2:65322585-65322604 MS.gene07302:CDS 50.0%
!! ATCAATTTTGGGGACCCGAG+AGG - chr1.2:65322564-65322583 MS.gene07302:CDS 50.0%
AAACGCTCCTGCACCATTGG+GGG + chr1.2:65322757-65322776 None:intergenic 55.0%
ACCCTGACGTGAACTCCTCT+CGG + chr1.2:65322582-65322601 None:intergenic 55.0%
CCAGCTGTAACTGATCCTCC+GGG + chr1.2:65323172-65323191 None:intergenic 55.0%
CCGGAGAGCAAAGAGTTCGA+AGG + chr1.2:65321822-65321841 None:intergenic 55.0%
CGCCAATCCAACACAGACAG+TGG + chr1.2:65323370-65323389 None:intergenic 55.0%
CGTCAACTGCTCCACCACAA+CGG + chr1.2:65322910-65322929 None:intergenic 55.0%
GAAACGCTCCTGCACCATTG+GGG + chr1.2:65322758-65322777 None:intergenic 55.0%
GAGAAGAAGAACCGGTGGTG+GGG + chr1.2:65321779-65321798 None:intergenic 55.0%
GGAAACGCTCCTGCACCATT+GGG + chr1.2:65322759-65322778 None:intergenic 55.0%
GTGAGAAGAAGAACCGGTGG+TGG + chr1.2:65321781-65321800 None:intergenic 55.0%
TGGAAACGCTCCTGCACCAT+TGG + chr1.2:65322760-65322779 None:intergenic 55.0%
TGGCCCTTGTCCTTGTGCAT+AGG + chr1.2:65322734-65322753 None:intergenic 55.0%
TTACCGAGGTTTCCTCCCTC+GGG - chr1.2:65322383-65322402 MS.gene07302:CDS 55.0%
! AGAAGAAGAACCGGTGGTGG+GGG + chr1.2:65321778-65321797 None:intergenic 55.0%
!! TCTGTGTTGGATTGGCGGCT+AGG - chr1.2:65323373-65323392 MS.gene07302:intron 55.0%
ACCCGAGAGGAGTTCACGTC+AGG - chr1.2:65322577-65322596 MS.gene07302:CDS 60.0%
CCCGAGAGGAGTTCACGTCA+GGG - chr1.2:65322578-65322597 MS.gene07302:CDS 60.0%
CCCGGAGGATCAGTTACAGC+TGG - chr1.2:65323169-65323188 MS.gene07302:intron 60.0%
CCCTGACGTGAACTCCTCTC+GGG + chr1.2:65322581-65322600 None:intergenic 60.0%
CCTCTTCTTTCCCCCACCAC+CGG - chr1.2:65321765-65321784 MS.gene07302:intron 60.0%
TACCGAGGTTTCCTCCCTCG+GGG - chr1.2:65322384-65322403 MS.gene07302:CDS 60.0%
AGGACAAGGGCCACCCCCAA+TGG - chr1.2:65322741-65322760 MS.gene07302:intron 65.0%
CTACGGGAAGGGGGTCCTTC+AGG + chr1.2:65322410-65322429 None:intergenic 65.0%
TTTCCTCCCTCGGGGCCTGA+AGG - chr1.2:65322392-65322411 MS.gene07302:intron 65.0%
!! CCGGTGGTGGGGGAAAGAAG+AGG + chr1.2:65321768-65321787 None:intergenic 65.0%
AGGGCCACCCCCAATGGTGC+AGG - chr1.2:65322747-65322766 MS.gene07302:intron 70.0%
CGCTCCTGCACCATTGGGGG+TGG + chr1.2:65322754-65322773 None:intergenic 70.0%
GGCCCCGAGGGAGGAAACCT+CGG + chr1.2:65322389-65322408 None:intergenic 70.0%
AGGGGGTCCTTCAGGCCCCG+AGG + chr1.2:65322402-65322421 None:intergenic 75.0%
GGGGGTCCTTCAGGCCCCGA+GGG + chr1.2:65322401-65322420 None:intergenic 75.0%
GGTCCTTCAGGCCCCGAGGG+AGG + chr1.2:65322398-65322417 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 65321714 65323841 65321714 ID=MS.gene07302
chr1.2 mRNA 65321714 65323841 65321714 ID=MS.gene07302.t1;Parent=MS.gene07302
chr1.2 exon 65323714 65323841 65323714 ID=MS.gene07302.t1.exon1;Parent=MS.gene07302.t1
chr1.2 CDS 65323714 65323841 65323714 ID=cds.MS.gene07302.t1;Parent=MS.gene07302.t1
chr1.2 exon 65322940 65323059 65322940 ID=MS.gene07302.t1.exon2;Parent=MS.gene07302.t1
chr1.2 CDS 65322940 65323059 65322940 ID=cds.MS.gene07302.t1;Parent=MS.gene07302.t1
chr1.2 exon 65322748 65322862 65322748 ID=MS.gene07302.t1.exon3;Parent=MS.gene07302.t1
chr1.2 CDS 65322748 65322862 65322748 ID=cds.MS.gene07302.t1;Parent=MS.gene07302.t1
chr1.2 exon 65322564 65322656 65322564 ID=MS.gene07302.t1.exon4;Parent=MS.gene07302.t1
chr1.2 CDS 65322564 65322656 65322564 ID=cds.MS.gene07302.t1;Parent=MS.gene07302.t1
chr1.2 exon 65322365 65322410 65322365 ID=MS.gene07302.t1.exon5;Parent=MS.gene07302.t1
chr1.2 CDS 65322365 65322410 65322365 ID=cds.MS.gene07302.t1;Parent=MS.gene07302.t1
chr1.2 exon 65322161 65322233 65322161 ID=MS.gene07302.t1.exon6;Parent=MS.gene07302.t1
chr1.2 CDS 65322161 65322233 65322161 ID=cds.MS.gene07302.t1;Parent=MS.gene07302.t1
chr1.2 exon 65321714 65321741 65321714 ID=MS.gene07302.t1.exon7;Parent=MS.gene07302.t1
chr1.2 CDS 65321714 65321741 65321714 ID=cds.MS.gene07302.t1;Parent=MS.gene07302.t1
Gene Sequence

>MS.gene07302

ATGTCTCATTCTTCTCTTCTCACCCCCTCTTCCTCTCTTACACTCAACTCTCCTCTTCTTTCCCCCACCACCGGTTCTTCTTCTCACTCTCACAATCTTTCTCTTCCTTCGAACTCTTTGCTCTCCGGGTATGTATATTTTTCTTTCCTTGTCTTTTCTTCAATTTCAATTTTTTATAACAACCCTTGAACTTATTCCTAACCTTTTCTTTTAACTCAATTTTAATTCCTGGTAAAGTTCTTATTTTTATTTCATATCTATAATTTCGAGATTTTTTTTTCCTTCCAATTTGTGGGTATTGAGTTTTTGTTGTTTGTTTACTTGTTTCTTTGTTTTGGGTTATTAGGTTCATACAAAATTGCCCCTTTTTAAATTTTGCTAGTAGGGTTTGGGTGAAATTGATTTTTTTTGAAATTCATTCTTGTGTTGTATTGTATCAAACTGAAAAATGATGCAAATATTATGATAAATTTGAACTGGCTTATTCTTGAGACAAATGAATAATCTGGGTTGGTGTAAAAGTCTTTCAATTTTTATTAGTTTAAGCTCAAATTAACCAAGTGATTAATTAGCTCTAGTTAGGACAAATCTTTTATGAGAATTTGAGTTCAAATCGTGGTTAAAACAATTATTTGCCAGACTTTACTTACTTTCTGGTTGAACTCCAAATTACCGAGGTTTCCTCCCTCGGGGCCTGAAGGACCCCCTTCCCGTAGAATATTCTATGCTATTTTTTATATTTTACTTCTACTAAGGGAATAAAACCATGCTGTGATTTGCAGGAAATTGAGGAAATCTGTAGAATTTAGAAAGATTACCCGAAAATCTGCCGTGAAAGCTGTGTATGGTGATCAATTTTGGGGACCCGAGAGGAGTTCACGTCAGGGTATTTGGTCCATAAGGTAGATTTTTGCTTGTTAATTTGTTTAATCTGTTAACTTTAGTCTTATTTATTGACTTGATATATGTGTTCTTCTAGGGAGGATTTGGGAGTTCCGTCTTCGCCTTATTTTCCTACCTATGCACAAGGACAAGGGCCACCCCCAATGGTGCAGGAGCGTTTCCAGAGTGTTATAAGTCAACTTTTCCAATATGTAAGGATTTTTTACTCTTGGCGTTGCTTAATGTTGAGTGTTTAGTTTATGTTTAGTCCTTCGATTATTGATAATCTATTTTGTTATTTAGAGAATAATCCGTTGTGGTGGAGCAGTTGACGATGATATGGCAAATATCATAGTTTCTCAGCTCCTTTATCTTGATGCTGTTGATCCAAACAAGGTTTGTCCGTCGTTTTTTAGTTGATCAAAGCTCGTGGTTATGTATACCTTTATCTATATAAAGTGATCCTTTGCATTTCTAGAGATGTCATCAAACACATGCATTTTGATATGGCTATGATTTTGAGTTTATCTTATCTACTATGTTGAACAGGATATTGTCATGTATGTAAATTCTCCCGGAGGATCAGTTACAGCTGGTAATAATTTTCTTATGCAACTATCTTGCACTTGTTTGTATATTTATTTTAATTACATGTAGTCTAAACATAGGCTAAGAAAATATCATTTCATTCAAAAAGTAATACACTTTCTTTGTTTTTGTTCTTAGGTATGGCAATATTTGATACAATGAGGCATATCAGACCTGATGTGTCCACTGTCTGTGTTGGATTGGCGGCTAGGTAATATTTCATTCTTCTTGTGGGTAATAATACATAATTGCTGATAAGTTACCACTCACTATCAGTAAAAGGGTAAAAGTCTATCCTAAATTTCATGTACACACAAAAAACTTATTCACTTTTTGTTTTGCTGAGTTCTAGGAGAATTTAAGAAGATGAATTAAAACCCTCACTTGCACACTGCCTATTAAGCCTATCTTTTTTTAGATAATTATTTCTTATCTATTTGTGTTTATTTAATTAGTGTGATTGATCGCCCTCATACACACCCCTTTTGTCATTTTCTATGTTTCCTGTCGCTAGTATTCTATGAAACCCCGACATAGATAGGGGGATACAGGACATTGACACAGACACATCGATATGAACAAAATTTAAAATATAGGATACCAGGATTTGTTGAGACTTGAGATCATGAGAGGAGTTAGTCTCAACCAATCACGTGTG
Protein sequence

>MS.gene07302.t1

MSHSSLLTPSSSLTLNSPLLSPTTGSSSHSHNLSLPSNSLLSGKLRKSVEFRKITRKSAVKAVYGDQFWGPERSSRQGIWSIREDLGVPSSPYFPTYAQGQGPPPMVQERFQSVISQLFQYRIIRCGGAVDDDMANIIVSQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIRPDVSTVCVGLAARGVSLNQSRV