Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20813.t1 | XP_003591392.1 | 98.6 | 141 | 2 | 0 | 1 | 141 | 49 | 189 | 1.10E-73 | 285.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20813.t1 | Q9S834 | 85.0 | 140 | 21 | 0 | 2 | 141 | 53 | 192 | 3.9e-64 | 245.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20813.t1 | G7ICX2 | 98.6 | 141 | 2 | 0 | 1 | 141 | 49 | 189 | 7.9e-74 | 285.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene20813 | 0.877402 | 6.47E-69 | -1.69E-46 |
| MS.gene049217 | MS.gene20813 | 0.809173 | 2.16E-50 | -1.69E-46 |
| MS.gene04921 | MS.gene20813 | -0.801056 | 1.08E-48 | -1.69E-46 |
| MS.gene049222 | MS.gene20813 | 0.874035 | 9.25E-68 | -1.69E-46 |
| MS.gene049232 | MS.gene20813 | 0.855854 | 4.77E-62 | -1.69E-46 |
| MS.gene049257 | MS.gene20813 | 0.816263 | 6.05E-52 | -1.69E-46 |
| MS.gene049285 | MS.gene20813 | 0.853896 | 1.76E-61 | -1.69E-46 |
| MS.gene049320 | MS.gene20813 | 0.834034 | 3.78E-56 | -1.69E-46 |
| MS.gene049609 | MS.gene20813 | 0.813386 | 2.63E-51 | -1.69E-46 |
| MS.gene049627 | MS.gene20813 | 0.820724 | 5.90E-53 | -1.69E-46 |
| MS.gene049633 | MS.gene20813 | 0.87754 | 5.79E-69 | -1.69E-46 |
| MS.gene049865 | MS.gene20813 | 0.85549 | 6.09E-62 | -1.69E-46 |
| MS.gene05010 | MS.gene20813 | 0.847192 | 1.35E-59 | -1.69E-46 |
| MS.gene050163 | MS.gene20813 | 0.832112 | 1.14E-55 | -1.69E-46 |
| MS.gene050165 | MS.gene20813 | 0.8418 | 3.82E-58 | -1.69E-46 |
| MS.gene050170 | MS.gene20813 | 0.918052 | 2.51E-86 | -1.69E-46 |
| MS.gene050323 | MS.gene20813 | 0.876536 | 1.29E-68 | -1.69E-46 |
| MS.gene050333 | MS.gene20813 | 0.80887 | 2.50E-50 | -1.69E-46 |
| MS.gene050338 | MS.gene20813 | -0.806129 | 9.55E-50 | -1.69E-46 |
| MS.gene050403 | MS.gene20813 | 0.823457 | 1.37E-53 | -1.69E-46 |
| MS.gene050444 | MS.gene20813 | 0.820832 | 5.57E-53 | -1.69E-46 |
| MS.gene050496 | MS.gene20813 | 0.889349 | 2.62E-73 | -1.69E-46 |
| MS.gene050504 | MS.gene20813 | 0.882834 | 7.47E-71 | -1.69E-46 |
| MS.gene050518 | MS.gene20813 | 0.864452 | 1.20E-64 | -1.69E-46 |
| MS.gene050538 | MS.gene20813 | 0.801234 | 9.92E-49 | -1.69E-46 |
| MS.gene050577 | MS.gene20813 | 0.891332 | 4.38E-74 | -1.69E-46 |
| MS.gene050612 | MS.gene20813 | 0.873018 | 2.03E-67 | -1.69E-46 |
| MS.gene050647 | MS.gene20813 | -0.824744 | 6.84E-54 | -1.69E-46 |
| MS.gene050675 | MS.gene20813 | 0.852065 | 5.90E-61 | -1.69E-46 |
| MS.gene050697 | MS.gene20813 | 0.807652 | 4.55E-50 | -1.69E-46 |
| MS.gene050699 | MS.gene20813 | 0.846654 | 1.90E-59 | -1.69E-46 |
| MS.gene050747 | MS.gene20813 | 0.84533 | 4.34E-59 | -1.69E-46 |
| MS.gene050772 | MS.gene20813 | 0.822572 | 2.21E-53 | -1.69E-46 |
| MS.gene05080 | MS.gene20813 | 0.850912 | 1.25E-60 | -1.69E-46 |
| MS.gene05081 | MS.gene20813 | 0.833201 | 6.11E-56 | -1.69E-46 |
| MS.gene050868 | MS.gene20813 | 0.896699 | 2.87E-76 | -1.69E-46 |
| MS.gene050871 | MS.gene20813 | 0.894262 | 2.91E-75 | -1.69E-46 |
| MS.gene050876 | MS.gene20813 | 0.857962 | 1.14E-62 | -1.69E-46 |
| MS.gene050904 | MS.gene20813 | 0.943162 | 1.98E-102 | -1.69E-46 |
| MS.gene050950 | MS.gene20813 | 0.845213 | 4.67E-59 | -1.69E-46 |
| MS.gene050986 | MS.gene20813 | 0.862358 | 5.36E-64 | -1.69E-46 |
| MS.gene051138 | MS.gene20813 | -0.82807 | 1.10E-54 | -1.69E-46 |
| MS.gene051268 | MS.gene20813 | 0.85981 | 3.20E-63 | -1.69E-46 |
| MS.gene051317 | MS.gene20813 | 0.901044 | 3.99E-78 | -1.69E-46 |
| MS.gene051551 | MS.gene20813 | 0.822674 | 2.09E-53 | -1.69E-46 |
| MS.gene051651 | MS.gene20813 | -0.819638 | 1.05E-52 | -1.69E-46 |
| MS.gene051734 | MS.gene20813 | 0.810123 | 1.35E-50 | -1.69E-46 |
| MS.gene051838 | MS.gene20813 | 0.84897 | 4.37E-60 | -1.69E-46 |
| MS.gene051874 | MS.gene20813 | 0.810173 | 1.31E-50 | -1.69E-46 |
| MS.gene051941 | MS.gene20813 | 0.836241 | 1.05E-56 | -1.69E-46 |
| MS.gene052066 | MS.gene20813 | 0.816092 | 6.61E-52 | -1.69E-46 |
| MS.gene052129 | MS.gene20813 | -0.810433 | 1.16E-50 | -1.69E-46 |
| MS.gene052164 | MS.gene20813 | 0.855338 | 6.75E-62 | -1.69E-46 |
| MS.gene052482 | MS.gene20813 | 0.88282 | 7.56E-71 | -1.69E-46 |
| MS.gene052486 | MS.gene20813 | 0.891118 | 5.32E-74 | -1.69E-46 |
| MS.gene052494 | MS.gene20813 | -0.841645 | 4.19E-58 | -1.69E-46 |
| MS.gene052503 | MS.gene20813 | 0.842831 | 2.03E-58 | -1.69E-46 |
| MS.gene052506 | MS.gene20813 | 0.811926 | 5.48E-51 | -1.69E-46 |
| MS.gene052606 | MS.gene20813 | -0.84973 | 2.68E-60 | -1.69E-46 |
| MS.gene052822 | MS.gene20813 | 0.814683 | 1.36E-51 | -1.69E-46 |
| MS.gene052823 | MS.gene20813 | 0.845034 | 5.22E-59 | -1.69E-46 |
| MS.gene052825 | MS.gene20813 | 0.846383 | 2.25E-59 | -1.69E-46 |
| MS.gene052870 | MS.gene20813 | 0.809176 | 2.15E-50 | -1.69E-46 |
| MS.gene052872 | MS.gene20813 | 0.822938 | 1.81E-53 | -1.69E-46 |
| MS.gene052966 | MS.gene20813 | 0.873464 | 1.44E-67 | -1.69E-46 |
| MS.gene053097 | MS.gene20813 | -0.838953 | 2.12E-57 | -1.69E-46 |
| MS.gene053203 | MS.gene20813 | 0.878703 | 2.27E-69 | -1.69E-46 |
| MS.gene053213 | MS.gene20813 | 0.809679 | 1.68E-50 | -1.69E-46 |
| MS.gene053284 | MS.gene20813 | 0.803819 | 2.91E-49 | -1.69E-46 |
| MS.gene053286 | MS.gene20813 | 0.812227 | 4.71E-51 | -1.69E-46 |
| MS.gene053420 | MS.gene20813 | -0.830144 | 3.47E-55 | -1.69E-46 |
| MS.gene053421 | MS.gene20813 | -0.820952 | 5.23E-53 | -1.69E-46 |
| MS.gene053436 | MS.gene20813 | 0.807168 | 5.77E-50 | -1.69E-46 |
| MS.gene053479 | MS.gene20813 | 0.866917 | 2.01E-65 | -1.69E-46 |
| MS.gene053480 | MS.gene20813 | 0.850901 | 1.26E-60 | -1.69E-46 |
| MS.gene053574 | MS.gene20813 | 0.826939 | 2.06E-54 | -1.69E-46 |
| MS.gene053626 | MS.gene20813 | 0.812573 | 3.96E-51 | -1.69E-46 |
| MS.gene053633 | MS.gene20813 | 0.816616 | 5.05E-52 | -1.69E-46 |
| MS.gene053824 | MS.gene20813 | 0.870285 | 1.64E-66 | -1.69E-46 |
| MS.gene054344 | MS.gene20813 | 0.850733 | 1.40E-60 | -1.69E-46 |
| MS.gene054408 | MS.gene20813 | 0.837166 | 6.10E-57 | -1.69E-46 |
| MS.gene054595 | MS.gene20813 | 0.853605 | 2.14E-61 | -1.69E-46 |
| MS.gene054600 | MS.gene20813 | 0.836739 | 7.84E-57 | -1.69E-46 |
| MS.gene054642 | MS.gene20813 | 0.81116 | 8.04E-51 | -1.69E-46 |
| MS.gene054706 | MS.gene20813 | -0.831725 | 1.42E-55 | -1.69E-46 |
| MS.gene05470 | MS.gene20813 | 0.906706 | 1.11E-80 | -1.69E-46 |
| MS.gene054851 | MS.gene20813 | -0.82324 | 1.54E-53 | -1.69E-46 |
| MS.gene054884 | MS.gene20813 | -0.819275 | 1.26E-52 | -1.69E-46 |
| MS.gene054954 | MS.gene20813 | 0.866991 | 1.90E-65 | -1.69E-46 |
| MS.gene055158 | MS.gene20813 | -0.846841 | 1.69E-59 | -1.69E-46 |
| MS.gene055202 | MS.gene20813 | -0.801331 | 9.47E-49 | -1.69E-46 |
| MS.gene055206 | MS.gene20813 | 0.807652 | 4.55E-50 | -1.69E-46 |
| MS.gene055224 | MS.gene20813 | 0.817716 | 2.85E-52 | -1.69E-46 |
| MS.gene055362 | MS.gene20813 | 0.842545 | 2.42E-58 | -1.69E-46 |
| MS.gene055502 | MS.gene20813 | 0.865518 | 5.57E-65 | -1.69E-46 |
| MS.gene055577 | MS.gene20813 | 0.882902 | 7.05E-71 | -1.69E-46 |
| MS.gene055741 | MS.gene20813 | 0.808321 | 3.28E-50 | -1.69E-46 |
| MS.gene055748 | MS.gene20813 | 0.897049 | 2.05E-76 | -1.69E-46 |
| MS.gene055750 | MS.gene20813 | 0.881182 | 2.97E-70 | -1.69E-46 |
| MS.gene055751 | MS.gene20813 | 0.900521 | 6.74E-78 | -1.69E-46 |
| MS.gene055776 | MS.gene20813 | 0.822171 | 2.73E-53 | -1.69E-46 |
| MS.gene05589 | MS.gene20813 | 0.922017 | 1.70E-88 | -1.69E-46 |
| MS.gene055941 | MS.gene20813 | 0.833491 | 5.17E-56 | -1.69E-46 |
| MS.gene056040 | MS.gene20813 | 0.838716 | 2.44E-57 | -1.69E-46 |
| MS.gene056166 | MS.gene20813 | 0.828458 | 8.89E-55 | -1.69E-46 |
| MS.gene056198 | MS.gene20813 | -0.800612 | 1.33E-48 | -1.69E-46 |
| MS.gene056266 | MS.gene20813 | 0.831196 | 1.91E-55 | -1.69E-46 |
| MS.gene056283 | MS.gene20813 | 0.827291 | 1.70E-54 | -1.69E-46 |
| MS.gene056387 | MS.gene20813 | 0.865829 | 4.45E-65 | -1.69E-46 |
| MS.gene056409 | MS.gene20813 | 0.802978 | 4.34E-49 | -1.69E-46 |
| MS.gene056446 | MS.gene20813 | 0.816572 | 5.16E-52 | -1.69E-46 |
| MS.gene056450 | MS.gene20813 | 0.828922 | 6.87E-55 | -1.69E-46 |
| MS.gene056504 | MS.gene20813 | 0.907319 | 5.76E-81 | -1.69E-46 |
| MS.gene056510 | MS.gene20813 | 0.869124 | 3.92E-66 | -1.69E-46 |
| MS.gene056549 | MS.gene20813 | 0.810777 | 9.74E-51 | -1.69E-46 |
| MS.gene056583 | MS.gene20813 | -0.81752 | 3.16E-52 | -1.69E-46 |
| MS.gene056610 | MS.gene20813 | 0.813643 | 2.31E-51 | -1.69E-46 |
| MS.gene056618 | MS.gene20813 | -0.832016 | 1.20E-55 | -1.69E-46 |
| MS.gene056657 | MS.gene20813 | 0.827639 | 1.40E-54 | -1.69E-46 |
| MS.gene056692 | MS.gene20813 | 0.871668 | 5.74E-67 | -1.69E-46 |
| MS.gene056694 | MS.gene20813 | -0.838203 | 3.31E-57 | -1.69E-46 |
| MS.gene056856 | MS.gene20813 | -0.807997 | 3.84E-50 | -1.69E-46 |
| MS.gene057019 | MS.gene20813 | 0.814081 | 1.85E-51 | -1.69E-46 |
| MS.gene057332 | MS.gene20813 | 0.822339 | 2.50E-53 | -1.69E-46 |
| MS.gene057658 | MS.gene20813 | 0.849824 | 2.52E-60 | -1.69E-46 |
| MS.gene057768 | MS.gene20813 | -0.840817 | 6.92E-58 | -1.69E-46 |
| MS.gene057831 | MS.gene20813 | 0.882416 | 1.06E-70 | -1.69E-46 |
| MS.gene057857 | MS.gene20813 | 0.812737 | 3.65E-51 | -1.69E-46 |
| MS.gene057872 | MS.gene20813 | 0.828862 | 7.10E-55 | -1.69E-46 |
| MS.gene05787 | MS.gene20813 | 0.853816 | 1.86E-61 | -1.69E-46 |
| MS.gene058157 | MS.gene20813 | 0.837041 | 6.57E-57 | -1.69E-46 |
| MS.gene058171 | MS.gene20813 | -0.842561 | 2.40E-58 | -1.69E-46 |
| MS.gene058173 | MS.gene20813 | -0.840972 | 6.30E-58 | -1.69E-46 |
| MS.gene058175 | MS.gene20813 | -0.845548 | 3.79E-59 | -1.69E-46 |
| MS.gene058334 | MS.gene20813 | -0.876946 | 9.31E-69 | -1.69E-46 |
| MS.gene058335 | MS.gene20813 | 0.807137 | 5.85E-50 | -1.69E-46 |
| MS.gene058338 | MS.gene20813 | 0.828648 | 8.00E-55 | -1.69E-46 |
| MS.gene05837 | MS.gene20813 | 0.88795 | 9.09E-73 | -1.69E-46 |
| MS.gene058418 | MS.gene20813 | -0.820434 | 6.87E-53 | -1.69E-46 |
| MS.gene058419 | MS.gene20813 | 0.848004 | 8.08E-60 | -1.69E-46 |
| MS.gene058544 | MS.gene20813 | 0.846697 | 1.85E-59 | -1.69E-46 |
| MS.gene058773 | MS.gene20813 | 0.845588 | 3.70E-59 | -1.69E-46 |
| MS.gene058838 | MS.gene20813 | 0.858251 | 9.37E-63 | -1.69E-46 |
| MS.gene059022 | MS.gene20813 | 0.853943 | 1.71E-61 | -1.69E-46 |
| MS.gene059023 | MS.gene20813 | 0.905217 | 5.42E-80 | -1.69E-46 |
| MS.gene059079 | MS.gene20813 | 0.803235 | 3.84E-49 | -1.69E-46 |
| MS.gene05912 | MS.gene20813 | 0.869218 | 3.65E-66 | -1.69E-46 |
| MS.gene05914 | MS.gene20813 | 0.850247 | 1.92E-60 | -1.69E-46 |
| MS.gene059181 | MS.gene20813 | 0.81092 | 9.07E-51 | -1.69E-46 |
| MS.gene059303 | MS.gene20813 | 0.813428 | 2.57E-51 | -1.69E-46 |
| MS.gene05934 | MS.gene20813 | 0.812765 | 3.60E-51 | -1.69E-46 |
| MS.gene059526 | MS.gene20813 | -0.85185 | 6.78E-61 | -1.69E-46 |
| MS.gene05960 | MS.gene20813 | -0.822885 | 1.86E-53 | -1.69E-46 |
| MS.gene059667 | MS.gene20813 | 0.843286 | 1.54E-58 | -1.69E-46 |
| MS.gene059677 | MS.gene20813 | 0.857673 | 1.39E-62 | -1.69E-46 |
| MS.gene059718 | MS.gene20813 | 0.83831 | 3.10E-57 | -1.69E-46 |
| MS.gene059756 | MS.gene20813 | 0.852922 | 3.36E-61 | -1.69E-46 |
| MS.gene059801 | MS.gene20813 | 0.839826 | 1.26E-57 | -1.69E-46 |
| MS.gene059837 | MS.gene20813 | 0.861865 | 7.60E-64 | -1.69E-46 |
| MS.gene059865 | MS.gene20813 | 0.878814 | 2.07E-69 | -1.69E-46 |
| MS.gene05996 | MS.gene20813 | 0.814546 | 1.46E-51 | -1.69E-46 |
| MS.gene06000 | MS.gene20813 | 0.917816 | 3.35E-86 | -1.69E-46 |
| MS.gene060187 | MS.gene20813 | -0.816429 | 5.55E-52 | -1.69E-46 |
| MS.gene06027 | MS.gene20813 | 0.817871 | 2.63E-52 | -1.69E-46 |
| MS.gene06042 | MS.gene20813 | -0.814555 | 1.45E-51 | -1.69E-46 |
| MS.gene060593 | MS.gene20813 | -0.828915 | 6.89E-55 | -1.69E-46 |
| MS.gene060595 | MS.gene20813 | -0.856626 | 2.83E-62 | -1.69E-46 |
| MS.gene060703 | MS.gene20813 | 0.843748 | 1.16E-58 | -1.69E-46 |
| MS.gene060762 | MS.gene20813 | 0.895689 | 7.55E-76 | -1.69E-46 |
| MS.gene060778 | MS.gene20813 | 0.877265 | 7.22E-69 | -1.69E-46 |
| MS.gene06077 | MS.gene20813 | 0.897272 | 1.65E-76 | -1.69E-46 |
| MS.gene060823 | MS.gene20813 | -0.806664 | 7.37E-50 | -1.69E-46 |
| MS.gene06092 | MS.gene20813 | 0.82879 | 7.39E-55 | -1.69E-46 |
| MS.gene060982 | MS.gene20813 | 0.909736 | 4.09E-82 | -1.69E-46 |
| MS.gene061044 | MS.gene20813 | -0.860859 | 1.54E-63 | -1.69E-46 |
| MS.gene061150 | MS.gene20813 | 0.839657 | 1.39E-57 | -1.69E-46 |
| MS.gene061231 | MS.gene20813 | 0.833983 | 3.90E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGTGTATGGTGATCAATTT+TGG | 0.210761 | 1.2:-65384366 | MS.gene20813:CDS |
| GTGTTCTTCTAGGGAGGATT+TGG | 0.266446 | 1.2:-65384237 | MS.gene20813:intron |
| GGAGTTCACGTCAGGGTATT+TGG | 0.314629 | 1.2:-65384333 | MS.gene20813:CDS |
| AACGACGGACAAACCTTGTT+TGG | 0.358156 | 1.2:+65383935 | None:intergenic |
| GATCAACAGCATCAAGATAA+AGG | 0.360915 | 1.2:+65383957 | None:intergenic |
| TGTTCTTCTAGGGAGGATTT+GGG | 0.362409 | 1.2:-65384236 | MS.gene20813:intron |
| TGTGCATAGGTAGGAAAATA+AGG | 0.368409 | 1.2:+65384200 | None:intergenic |
| GGAAACGCTCCTGCACCATT+GGG | 0.370261 | 1.2:+65384162 | None:intergenic |
| TGGAAACGCTCCTGCACCAT+TGG | 0.372062 | 1.2:+65384161 | None:intergenic |
| ACCAGCTGTAACTGATCCTC+CGG | 0.426032 | 1.2:+65383748 | None:intergenic |
| ACCCGAGAGGAGTTCACGTC+AGG | 0.435644 | 1.2:-65384341 | MS.gene20813:CDS |
| ATCACCATACACAGCTTTCA+CGG | 0.458968 | 1.2:+65384375 | None:intergenic |
| GTCAGGGTATTTGGTCCATA+AGG | 0.486578 | 1.2:-65384324 | MS.gene20813:intron |
| ACCCTGACGTGAACTCCTCT+CGG | 0.491382 | 1.2:+65384339 | None:intergenic |
| TGGCCCTTGTCCTTGTGCAT+AGG | 0.495346 | 1.2:+65384187 | None:intergenic |
| CACTGTCTGTGTTGGATTGG+CGG | 0.498037 | 1.2:-65383550 | MS.gene20813:CDS |
| AAACGCTCCTGCACCATTGG+GGG | 0.517765 | 1.2:+65384164 | None:intergenic |
| GTCCACTGTCTGTGTTGGAT+TGG | 0.520930 | 1.2:-65383553 | MS.gene20813:CDS |
| TGGAGCAGTTGACGATGATA+TGG | 0.521009 | 1.2:-65384002 | MS.gene20813:CDS |
| ACACAGACAGTGGACACATC+AGG | 0.523363 | 1.2:+65383561 | None:intergenic |
| GAAACGCTCCTGCACCATTG+GGG | 0.523580 | 1.2:+65384163 | None:intergenic |
| TCTGTGTTGGATTGGCGGCT+AGG | 0.523757 | 1.2:-65383545 | MS.gene20813:CDS |
| GATGTGTCCACTGTCTGTGT+TGG | 0.530369 | 1.2:-65383558 | MS.gene20813:CDS |
| CCCGGAGGATCAGTTACAGC+TGG | 0.538507 | 1.2:-65383749 | MS.gene20813:intron |
| CCTACCTATGCACAAGGACA+AGG | 0.541580 | 1.2:-65384191 | MS.gene20813:CDS |
| GTCATGTATGTAAATTCTCC+CGG | 0.542326 | 1.2:-65383767 | MS.gene20813:CDS |
| AAAGTTGACTTATAACACTC+TGG | 0.552761 | 1.2:+65384141 | None:intergenic |
| GTAGGAAAATAAGGCGAAGA+CGG | 0.567411 | 1.2:+65384209 | None:intergenic |
| CCCTGACGTGAACTCCTCTC+GGG | 0.567797 | 1.2:+65384340 | None:intergenic |
| TCTGCCGTGAAAGCTGTGTA+TGG | 0.585170 | 1.2:-65384379 | MS.gene20813:CDS |
| CCTTGTCCTTGTGCATAGGT+AGG | 0.585374 | 1.2:+65384191 | None:intergenic |
| TAGAGAATAATCCGTTGTGG+TGG | 0.606980 | 1.2:-65384022 | MS.gene20813:CDS |
| TGATGCTGTTGATCCAAACA+AGG | 0.607848 | 1.2:-65383948 | MS.gene20813:intron |
| CCCGAGAGGAGTTCACGTCA+GGG | 0.610211 | 1.2:-65384340 | MS.gene20813:CDS |
| CCAGCTGTAACTGATCCTCC+GGG | 0.618930 | 1.2:+65383749 | None:intergenic |
| CGCCAATCCAACACAGACAG+TGG | 0.626024 | 1.2:+65383551 | None:intergenic |
| TGGCAATATTTGATACAATG+AGG | 0.631433 | 1.2:-65383593 | MS.gene20813:CDS |
| ATTTAGAGAATAATCCGTTG+TGG | 0.633851 | 1.2:-65384025 | MS.gene20813:intron |
| CTACCTATGCACAAGGACAA+GGG | 0.671421 | 1.2:-65384190 | MS.gene20813:CDS |
| CGTCAACTGCTCCACCACAA+CGG | 0.680926 | 1.2:+65384011 | None:intergenic |
| ATGTATGTAAATTCTCCCGG+AGG | 0.710686 | 1.2:-65383764 | MS.gene20813:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAGATTATCAATAATCGA+AGG | + | chr1.2:65383894-65383913 | None:intergenic | 20.0% |
| !! | AGTAAAAAATCCTTACATAT+TGG | + | chr1.2:65383830-65383849 | None:intergenic | 20.0% |
| ! | ATTACATGTAGTCTAAACAT+AGG | - | chr1.2:65384268-65384287 | MS.gene20813:intron | 25.0% |
| ! | GGATCACTTTATATAGATAA+AGG | + | chr1.2:65384067-65384086 | None:intergenic | 25.0% |
| ! | TTGATATATGTGTTCTTCTA+GGG | - | chr1.2:65383698-65383717 | MS.gene20813:intron | 25.0% |
| ! | TTTGATCAACTAAAAAACGA+CGG | + | chr1.2:65384027-65384046 | None:intergenic | 25.0% |
| !!! | ATGTAAGGATTTTTTACTCT+TGG | - | chr1.2:65383832-65383851 | MS.gene20813:intron | 25.0% |
| !!! | CTTTCTTTGTTTTTGTTCTT+AGG | - | chr1.2:65384326-65384345 | MS.gene20813:CDS | 25.0% |
| !!! | TTTGTTTTTGTTCTTAGGTA+TGG | - | chr1.2:65384331-65384350 | MS.gene20813:CDS | 25.0% |
| ACAAGCAAAAATCTACCTTA+TGG | + | chr1.2:65383638-65383657 | None:intergenic | 30.0% | |
| ATTTAGAGAATAATCCGTTG+TGG | - | chr1.2:65383919-65383938 | MS.gene20813:intron | 30.0% | |
| CTTGATATATGTGTTCTTCT+AGG | - | chr1.2:65383697-65383716 | MS.gene20813:intron | 30.0% | |
| TCTTATCTACTATGTTGAAC+AGG | - | chr1.2:65384149-65384168 | MS.gene20813:CDS | 30.0% | |
| TGGCAATATTTGATACAATG+AGG | - | chr1.2:65384351-65384370 | MS.gene20813:CDS | 30.0% | |
| ! | AAAGTTGACTTATAACACTC+TGG | + | chr1.2:65383806-65383825 | None:intergenic | 30.0% |
| ! | GTCAACTTTTCCAATATGTA+AGG | - | chr1.2:65383817-65383836 | MS.gene20813:intron | 30.0% |
| ! | TGTGTATGGTGATCAATTTT+GGG | - | chr1.2:65383579-65383598 | MS.gene20813:CDS | 30.0% |
| !! | CAAACACATGCATTTTGATA+TGG | - | chr1.2:65384109-65384128 | MS.gene20813:intron | 30.0% |
| GATCAACAGCATCAAGATAA+AGG | + | chr1.2:65383990-65384009 | None:intergenic | 35.0% | |
| GTCATGTATGTAAATTCTCC+CGG | - | chr1.2:65384177-65384196 | MS.gene20813:CDS | 35.0% | |
| TGTGCATAGGTAGGAAAATA+AGG | + | chr1.2:65383747-65383766 | None:intergenic | 35.0% | |
| ! | ATATATGTGTTCTTCTAGGG+AGG | - | chr1.2:65383701-65383720 | MS.gene20813:intron | 35.0% |
| ! | CTGTGTATGGTGATCAATTT+TGG | - | chr1.2:65383578-65383597 | MS.gene20813:CDS | 35.0% |
| ! | GTGTATGGTGATCAATTTTG+GGG | - | chr1.2:65383580-65383599 | MS.gene20813:CDS | 35.0% |
| ! | TATTTTCCTACCTATGCACA+AGG | - | chr1.2:65383747-65383766 | MS.gene20813:intron | 35.0% |
| ! | TGACATCTCTAGAAATGCAA+AGG | + | chr1.2:65384088-65384107 | None:intergenic | 35.0% |
| !! | TTTTAGTTGATCAAAGCTCG+TGG | - | chr1.2:65384032-65384051 | MS.gene20813:intron | 35.0% |
| ATCACCATACACAGCTTTCA+CGG | + | chr1.2:65383572-65383591 | None:intergenic | 40.0% | |
| ATGTATGTAAATTCTCCCGG+AGG | - | chr1.2:65384180-65384199 | MS.gene20813:CDS | 40.0% | |
| GTAGGAAAATAAGGCGAAGA+CGG | + | chr1.2:65383738-65383757 | None:intergenic | 40.0% | |
| TAGAGAATAATCCGTTGTGG+TGG | - | chr1.2:65383922-65383941 | MS.gene20813:intron | 40.0% | |
| TGATGCTGTTGATCCAAACA+AGG | - | chr1.2:65383996-65384015 | MS.gene20813:CDS | 40.0% | |
| ! | TGTTCTTCTAGGGAGGATTT+GGG | - | chr1.2:65383708-65383727 | MS.gene20813:intron | 40.0% |
| AACGACGGACAAACCTTGTT+TGG | + | chr1.2:65384012-65384031 | None:intergenic | 45.0% | |
| CTACCTATGCACAAGGACAA+GGG | - | chr1.2:65383754-65383773 | MS.gene20813:CDS | 45.0% | |
| TGGAGCAGTTGACGATGATA+TGG | - | chr1.2:65383942-65383961 | MS.gene20813:intron | 45.0% | |
| ! | AGCTTTCACGGCAGATTTTC+GGG | + | chr1.2:65383560-65383579 | None:intergenic | 45.0% |
| ! | CAGCTTTCACGGCAGATTTT+CGG | + | chr1.2:65383561-65383580 | None:intergenic | 45.0% |
| ! | GTCAGGGTATTTGGTCCATA+AGG | - | chr1.2:65383620-65383639 | MS.gene20813:intron | 45.0% |
| ! | GTGTTCTTCTAGGGAGGATT+TGG | - | chr1.2:65383707-65383726 | MS.gene20813:intron | 45.0% |
| ACACAGACAGTGGACACATC+AGG | + | chr1.2:65384386-65384405 | None:intergenic | 50.0% | |
| ACCAGCTGTAACTGATCCTC+CGG | + | chr1.2:65384199-65384218 | None:intergenic | 50.0% | |
| CCTACCTATGCACAAGGACA+AGG | - | chr1.2:65383753-65383772 | MS.gene20813:CDS | 50.0% | |
| CCTTGTCCTTGTGCATAGGT+AGG | + | chr1.2:65383756-65383775 | None:intergenic | 50.0% | |
| GATGTGTCCACTGTCTGTGT+TGG | - | chr1.2:65384386-65384405 | MS.gene20813:CDS | 50.0% | |
| GTCCACTGTCTGTGTTGGAT+TGG | - | chr1.2:65384391-65384410 | MS.gene20813:CDS | 50.0% | |
| TCTGCCGTGAAAGCTGTGTA+TGG | - | chr1.2:65383565-65383584 | MS.gene20813:CDS | 50.0% | |
| ! | CACTGTCTGTGTTGGATTGG+CGG | - | chr1.2:65384394-65384413 | MS.gene20813:CDS | 50.0% |
| ! | GGAGTTCACGTCAGGGTATT+TGG | - | chr1.2:65383611-65383630 | MS.gene20813:intron | 50.0% |
| !! | ATCAATTTTGGGGACCCGAG+AGG | - | chr1.2:65383590-65383609 | MS.gene20813:CDS | 50.0% |
| AAACGCTCCTGCACCATTGG+GGG | + | chr1.2:65383783-65383802 | None:intergenic | 55.0% | |
| ACCCTGACGTGAACTCCTCT+CGG | + | chr1.2:65383608-65383627 | None:intergenic | 55.0% | |
| CCAGCTGTAACTGATCCTCC+GGG | + | chr1.2:65384198-65384217 | None:intergenic | 55.0% | |
| CGCCAATCCAACACAGACAG+TGG | + | chr1.2:65384396-65384415 | None:intergenic | 55.0% | |
| CGTCAACTGCTCCACCACAA+CGG | + | chr1.2:65383936-65383955 | None:intergenic | 55.0% | |
| GAAACGCTCCTGCACCATTG+GGG | + | chr1.2:65383784-65383803 | None:intergenic | 55.0% | |
| GGAAACGCTCCTGCACCATT+GGG | + | chr1.2:65383785-65383804 | None:intergenic | 55.0% | |
| TGGAAACGCTCCTGCACCAT+TGG | + | chr1.2:65383786-65383805 | None:intergenic | 55.0% | |
| TGGCCCTTGTCCTTGTGCAT+AGG | + | chr1.2:65383760-65383779 | None:intergenic | 55.0% | |
| !! | TCTGTGTTGGATTGGCGGCT+AGG | - | chr1.2:65384399-65384418 | MS.gene20813:CDS | 55.0% |
| ACCCGAGAGGAGTTCACGTC+AGG | - | chr1.2:65383603-65383622 | MS.gene20813:intron | 60.0% | |
| CCCGAGAGGAGTTCACGTCA+GGG | - | chr1.2:65383604-65383623 | MS.gene20813:intron | 60.0% | |
| CCCGGAGGATCAGTTACAGC+TGG | - | chr1.2:65384195-65384214 | MS.gene20813:CDS | 60.0% | |
| CCCTGACGTGAACTCCTCTC+GGG | + | chr1.2:65383607-65383626 | None:intergenic | 60.0% | |
| AGGACAAGGGCCACCCCCAA+TGG | - | chr1.2:65383767-65383786 | MS.gene20813:CDS | 65.0% | |
| AGGGCCACCCCCAATGGTGC+AGG | - | chr1.2:65383773-65383792 | MS.gene20813:CDS | 70.0% | |
| CGCTCCTGCACCATTGGGGG+TGG | + | chr1.2:65383780-65383799 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 65383542 | 65384424 | 65383542 | ID=MS.gene20813 |
| chr1.2 | mRNA | 65383542 | 65384424 | 65383542 | ID=MS.gene20813.t1;Parent=MS.gene20813 |
| chr1.2 | exon | 65384325 | 65384424 | 65384325 | ID=MS.gene20813.t1.exon1;Parent=MS.gene20813.t1 |
| chr1.2 | CDS | 65384325 | 65384424 | 65384325 | ID=cds.MS.gene20813.t1;Parent=MS.gene20813.t1 |
| chr1.2 | exon | 65384133 | 65384247 | 65384133 | ID=MS.gene20813.t1.exon2;Parent=MS.gene20813.t1 |
| chr1.2 | CDS | 65384133 | 65384247 | 65384133 | ID=cds.MS.gene20813.t1;Parent=MS.gene20813.t1 |
| chr1.2 | exon | 65383949 | 65384041 | 65383949 | ID=MS.gene20813.t1.exon3;Parent=MS.gene20813.t1 |
| chr1.2 | CDS | 65383949 | 65384041 | 65383949 | ID=cds.MS.gene20813.t1;Parent=MS.gene20813.t1 |
| chr1.2 | exon | 65383750 | 65383795 | 65383750 | ID=MS.gene20813.t1.exon4;Parent=MS.gene20813.t1 |
| chr1.2 | CDS | 65383750 | 65383795 | 65383750 | ID=cds.MS.gene20813.t1;Parent=MS.gene20813.t1 |
| chr1.2 | exon | 65383542 | 65383618 | 65383542 | ID=MS.gene20813.t1.exon5;Parent=MS.gene20813.t1 |
| chr1.2 | CDS | 65383542 | 65383618 | 65383542 | ID=cds.MS.gene20813.t1;Parent=MS.gene20813.t1 |
| Gene Sequence |
| Protein sequence |
